CN102217485B - Transplanting method for peanut tissue cultured seedlings and nutrient soil - Google Patents
Transplanting method for peanut tissue cultured seedlings and nutrient soil Download PDFInfo
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- CN102217485B CN102217485B CN 201110132317 CN201110132317A CN102217485B CN 102217485 B CN102217485 B CN 102217485B CN 201110132317 CN201110132317 CN 201110132317 CN 201110132317 A CN201110132317 A CN 201110132317A CN 102217485 B CN102217485 B CN 102217485B
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Abstract
The invention discloses a transplanting method for peanut tissue cultured seedlings and nutrient soil. The transplanting method comprises the steps of seedling adaptation before transplanting, preparation of transplanting nutrient soil, selection of transplanting containers, reasonable transplanting, maintenance management after transplanting and the like. Meanwhile, the invention also provides the nutrient soil for transplanting the peanut tissue cultured seedlings. By using the transplanting method for the peanut tissue cultured seedlings and the nutrient soil, the transplanting survival rate can reach over 95 percent, the transplanted peanut plants are robust and can normally flower and fruit, and average 7 to 13 fruits are grown in each plant. The bottleneck problem of low survival rate of the peanut tissue cultured seedlings is solved, and a foundation is laid for the development of peanut tissue culture.
Description
Technical field
The transplanting method that the present invention relates to a kind of peanut tissue culture seedlings reaches the Nutrition Soil that is used for the peanut tissue culture transplantation of seedlings, belongs to peanut tissue culture technique field.
Background technology
Peanut is the important oil crop of China, economic crops and food crop, and the peanut area is planted more than 6,500 ten thousand mu throughout the year by China, and average yield per mu is about 225 kilograms, and gross output is about 1,400 ten thousand tons.China's peanut is in breeding, cultivation, the research of plant protection and processing and other fields all is in the leading level in the world, but because the hereditary basis of peanut is narrow, resistance is poor, the insect pest of susceptible Various Diseases, cause the rooting rate of peanut tissue culture regrowth low, namely enable to take root, its transplanting survival rate is also very low, especially the peanut regrowth with merit that obtains by technology such as transgenosis and screening mutants, can not guarantee to transplant with high-servival rate, seriously limited the development of the cultivation of peanut tissue and transgenic technology, so the peanut tissue is cultivated and transgenic technology is the global problem that the Peanut Research worker faces all the time.
Summary of the invention
In view of the deficiencies in the prior art, first purpose of the present invention is by traditional peanut tissue culture technique is improved, thereby provide a kind of transplanting method of peanut tissue culture seedlings, to overcome low, the high difficult problem of group training cost of peanut tissue culture seedlings transplanting survival rate in the prior art.
First purpose of the present invention is achieved through the following technical solutions:
A kind of transplanting method of peanut tissue culture seedlings is characterized in that: may further comprise the steps successively:
The peanut tissue culture seedlings that (1) will be grown in the blake bottle is put into the land for growing field crops, removes the blake bottle sealing, adds 2-3mm sterile water highly at medium, sterile water of replacing in 2 days, at room temperature hardening 6-7 days;
(2) in Nutrition Soil, dig a dark 7-10cm, the aperture of wide 7-10cm, take out from blake bottle with the group training seedling of tweezers after with hardening, plant in aperture, cover Nutrition Soil, gently water permeable, and keep humidity more than 90%, wherein said Nutrition Soil is prepared from as follows: with vegetable garden soil, humus soil, ash, sieved river sand by 1: 1: 1: 1 mass ratio mixes rear high pressure steam sterilization, then 50% carbendazol wettable powder that adds 0.01% mass ratio, mix, in the flexible plastic nutritive cube of the 11cm * 11cm that packs into～15cm * 15cm;
(3) training of the group after transplanting seedling is placed in the shade, humidity in the maintenance canopy is at 90-100%, after the transplanting front 7 days, to keep humidity and temperature, transplant after 7 days with the sterile water processing of spraying every morning, spraying in per 2 days 1 time, and 1 500-700 carbendazim solution doubly of spraying, after 20 days, remove shade net.
The transplanting method of above-mentioned a kind of peanut tissue culture seedlings in the wherein said step (2), after taking out from blake bottle with the group training seedling of tweezers after with hardening, all rinses the agar of root adhesion with sterile water.
Second purpose of the present invention is to provide a kind of Nutrition Soil for the peanut tissue culture transplantation of seedlings, and concrete technical scheme is as follows:
A kind of Nutrition Soil for the peanut tissue culture transplantation of seedlings, it is characterized in that: be prepared from as follows: with vegetable garden soil, humus soil, ash, the river sand that sieves by 1: 1: 1: 1 mass ratio mixes rear high pressure steam sterilization, then add 50% carbendazol wettable powder of 0.01% mass ratio, mix.
Above-mentioned a kind of Nutrition Soil for the peanut tissue culture transplantation of seedlings is loaded into the flexible plastic nutritive cube of 11cm * 11cm～15cm * 15cm.
Compared with prior art, the present invention has following advantage and significant progressive: the transplanting method that utilizes peanut tissue culture seedlings provided by the invention, the transplanting survival rate of peanut tissue culture seedlings is brought up to more than 95%, peanut plant is produced vigorous after transplanting, can blossom and bear fruit normally, average every strain is 7-13 (specifically referring to specific embodiment 1-2) as a result.This shows, the invention solves the problem that restriction peanut tissue is cultivated transplanting survival rate, obtained significant economic benefit and social benefit.
Embodiment
Below be specific embodiments of the invention, technical scheme of the present invention is done further the description, but protection scope of the present invention is not limited to these embodiment.Every do not deviate from the change of the present invention design or be equal to substitute include within protection scope of the present invention.
The transplanting of embodiment 1 peanut tissue culture seedlings
Before the peanut tissue culture transplantation of seedlings, flower in the blake bottle is educated No. 23 group training seedlings carry out hardening, blake bottle is taken the land for growing field crops from culturing room to be placed, then the blake bottle sealing is removed, when removing sealing, add the 2mm sterile water at medium, to keep humidity and temperature, sterile water was changed once in 2 days, and at room temperature hardening is 6 days.In hardening with vegetable garden soil, humus soil, ash, the river sand that sieves according to mass ratio 1: 1: 1: 1 ratio mixes, disinfection in high-pressure sterilizing pot, then add 50% carbendazol wettable powder according to 0.01% mass ratio and mix, in the flexible plastic nutritive cube of the 11cm * 11cm that then Nutrition Soil packed into.At first will organize the training seedling during transplanting and take out from blake bottle, and will operate carefully with tweezers when getting, and be sure not root system is damaged, the agar that then root is adhered to all rinses with sterile water.During transplanting Nutrition Soil is dug a dark 7cm, the aperture of wide 7cm, and then plant planted down allows the root spread apart, and prevents from injuring seedling.Cover gently Nutrition Soil, with thin and delicate root system and the Gen Mao of antisitic defect test-tube plantlet.Transplant altogether 100 strain flowers and educate group training seedling No. 23.Gently water permeablely after the transplanting, again seedling is moved in the environment of high humidity, guarantee that space humidity remains on more than 90%.Group after transplanting training seedling is placed in the shade, keeps humidity in the canopy at 90-100%, transplants front 7 days, and every morning is with sterile water processings of spraying, with the maintenance humidity and temperature.Transplant after 7 days, spraying in per 2 days 1 time, and 500 times the carbendazim solution of spraying 1 time after 20 days, remove shade net.97 strain groups training seedling is at large field normal growth, and transplanting survival rate reaches 97%.After gathering in the crops autumn, 11.6 of average every strain results.
The transplanting of embodiment 2 peanut tissue culture seedlings
Before the peanut tissue culture transplantation of seedlings, flower in the blake bottle is educated No. 30 group training seedlings carry out hardening, blake bottle is taken the land for growing field crops from culturing room to be placed, then the blake bottle sealing is removed, when removing sealing, add the 3mm sterile water at medium, to keep humidity and temperature, sterile water was changed once in 2 days, and at room temperature hardening is 7 days.In hardening with vegetable garden soil, humus soil, ash, the river sand that sieves according to mass ratio 1: 1: 1: 1 ratio mixes, disinfection in high-pressure sterilizing pot, then add 50% carbendazol wettable powder according to 0.01% mass ratio and mix, in the flexible plastic nutritive cube of the 15cm * 15cm that then Nutrition Soil packed into.At first will organize the training seedling during transplanting and take out from blake bottle, and will operate carefully with tweezers when getting, and be sure not root system is damaged, the agar that then root is adhered to all rinses with sterile water.During transplanting Nutrition Soil is dug a dark 10cm, the aperture of wide 10cm, and then plant planted down allows the root spread apart, and prevents from injuring seedling.Cover gently Nutrition Soil, with thin and delicate root system and the Gen Mao of antisitic defect test-tube plantlet.Transplant altogether 260 strain flowers and educate group training seedling No. 30.Gently water permeablely after the transplanting, again seedling is moved in the environment of high humidity, guarantee that space humidity remains on more than 90%.Group after transplanting training seedling is placed in the shade, keeps humidity in the canopy at 90-100%, transplants front 7 days, and every morning is with sterile water processings of spraying, with the maintenance humidity and temperature.Transplant after 7 days, spraying in per 2 days 1 time, and 700 times the carbendazim solution of spraying 1 time after 20 days, remove shade net.256 strain groups training seedling is at large field normal growth, and transplanting survival rate reaches 98.5%.After gathering in the crops autumn, 9.3 of average every strain results.
Claims (1)
1. the transplanting method of a peanut tissue culture seedlings is characterized in that: may further comprise the steps successively:
The peanut tissue culture seedlings that (1) will be grown in the blake bottle is put into the land for growing field crops, removes the blake bottle sealing, adds 2-3mm sterile water highly at medium, sterile water of replacing in 2 days, at room temperature hardening 6-7 days;
(2) in Nutrition Soil, dig a dark 7-10cm, the aperture of wide 7-10cm, take out from blake bottle with the group training seedling of tweezers after with hardening, plant in aperture, cover Nutrition Soil, gently water permeable, and keep humidity more than 90%, wherein said Nutrition Soil is prepared from as follows: with vegetable garden soil, humus soil, ash, the river sand that sieves mixes rear high pressure steam sterilization by the mass ratio of 1:1:1:1, then 50% carbendazol wettable powder that adds 0.01% mass ratio, mix, in the flexible plastic nutritive cube of the 11cm * 11cm that packs into～15cm * 15cm;
(3) training of the group after transplanting seedling is placed in the shade, humidity in the maintenance canopy is at 90-100%, after the transplanting front 7 days, to keep humidity and temperature, transplant after 7 days with the sterile water processing of spraying every morning, spraying in per 2 days 1 time, and 1 500-700 carbendazim solution doubly of spraying, after 20 days, remove shade net;
After taking out from blake bottle with the group training seedling of tweezers after with hardening in the described step (2), with sterile water the agar of root adhesion is all rinsed.
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| CN 201110132317 CN102217485B (en) | 2011-05-20 | 2011-05-20 | Transplanting method for peanut tissue cultured seedlings and nutrient soil |
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| CN 201110132317 CN102217485B (en) | 2011-05-20 | 2011-05-20 | Transplanting method for peanut tissue cultured seedlings and nutrient soil |
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| CN102217485A CN102217485A (en) | 2011-10-19 |
| CN102217485B true CN102217485B (en) | 2013-04-24 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630561B (en) * | 2012-03-22 | 2014-05-14 | 山东省花生研究所 | Method for transplanting peanut tissue culture seedlings |
| CN102577874B (en) * | 2012-03-23 | 2013-07-31 | 山东省花生研究所 | Method for transplanting transgenic peanut seedlings |
| CN102599058A (en) * | 2012-03-23 | 2012-07-25 | 山东省花生研究所 | Domestication method of peanut transgenic seedlings in bottles |
| CN104335775A (en) * | 2013-07-29 | 2015-02-11 | 林天国 | Seedling transplantation type planting method of peanut |
| CN103814736A (en) * | 2014-03-07 | 2014-05-28 | 安徽理工大学 | Cuckoo greenhouse seedling thinning transplantation method |
| CN103858771B (en) * | 2014-04-02 | 2015-09-30 | 山东省农业科学院生物技术研究中心 | A kind of transplanting method of corn gene plantlet in vitro |
| CN104151070A (en) * | 2014-07-31 | 2014-11-19 | 和县秀山美地农业科技有限公司 | Peanut culture substrate for preventing pest damage |
| CN105850461A (en) * | 2016-04-25 | 2016-08-17 | 吉林师范大学 | Transplanting method for purple sweet potato seedlings |
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| US6378247B1 (en) * | 2000-01-19 | 2002-04-30 | Tokyo University Of Agriculture | Method for afforestation of sands and the like |
| CN1951177A (en) * | 2006-09-26 | 2007-04-25 | 徐坤 | A seedling environment training method for ginger tissue cultured test-tube seedling |
| CN101165015A (en) * | 2006-10-17 | 2008-04-23 | 巩本楷 | Method for preparing flower culturing soil |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6378247B1 (en) * | 2000-01-19 | 2002-04-30 | Tokyo University Of Agriculture | Method for afforestation of sands and the like |
| CN1951177A (en) * | 2006-09-26 | 2007-04-25 | 徐坤 | A seedling environment training method for ginger tissue cultured test-tube seedling |
| CN101165015A (en) * | 2006-10-17 | 2008-04-23 | 巩本楷 | Method for preparing flower culturing soil |
Non-Patent Citations (2)
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| 吴中军等.不同移栽基质对生姜试管苗生长的影响.《西南大学学报(自然科学版)》.2010,第32卷(第04期), * |
| 张慧琴等.树莓试管苗移栽适应性试验.《浙江农业科学》.2002,(第03期), * |
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Inventor after: Yang Qingli Inventor after: Wang Jijun Inventor after: Yu Shanlin Inventor after: Zhu Feng Inventor after: Chen Mingna Inventor after: Chi Xiaoyuan Inventor after: Yang Zhen Inventor after: Pan Lijuan Inventor after: Chen Na Inventor before: Yang Qingli Inventor before: Yu Shanlin Inventor before: Zhu Feng Inventor before: Chen Mingna Inventor before: Chi Xiaoyuan Inventor before: Yang Zhen Inventor before: Pan Lijuan Inventor before: Chen Na |
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