CN108294051A - A kind of low temperature season evergreen tree callus cladding material and its processing method - Google Patents
A kind of low temperature season evergreen tree callus cladding material and its processing method Download PDFInfo
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- CN108294051A CN108294051A CN201810125218.8A CN201810125218A CN108294051A CN 108294051 A CN108294051 A CN 108294051A CN 201810125218 A CN201810125218 A CN 201810125218A CN 108294051 A CN108294051 A CN 108294051A
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- callus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G13/02—Protective coverings for plants; Coverings for the ground; Devices for laying-out or removing coverings
- A01G13/0237—Devices for protecting a specific part of a plant, e.g. roots, trunk or fruits
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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Abstract
The invention belongs to garden afforestation technology fields, and in particular to a kind of low temperature season evergreen tree callus cladding material and its processing method.The callus cladding material soaks cladding gauze by callus agent solution and obtains, and callus agent solution is made of following raw material：Seaweeds, brassin, vitamin C, potassium sorbate, potassium iodide, chitosan oligosaccharide citrate, plant hormone lysate, bacteria remover and glucose solution.Wherein, plant hormone lysate is the ethanol solution of gibberellin, heteroauxin, the basic element of cell division, contains streptomysin, thiophanate methyl and difenoconazole in bacteria remover, callus processing procedure includes debridement, wrapping and warming processing.Callus cladding material and callus processing method break branch wound face with very outstanding healing effect for the large size of winter low temperature weather aiphyllium.
Description
Technical field
The invention belongs to garden afforestation technology fields, and in particular to a kind of low temperature season evergreen tree callus coat material and
Its processing method.
Background technology
In urban afforestation field, the green tree species that northern area generally uses is cold-resistant defoliation seeds, and southern area
Aiphyllium can be selected to improve Afforestation Landscape effect.The cold tolerance of deciduous tree is stronger, in winter when leaf wither and fall after, tree
Body is more preferable to the bearing capacity of snowfall, the problem of accumulated snow be collapsed trees will not occur.And in southern area, since weather is relatively warm
It is warm, and snowfall intensity is weaker, therefore will not be gone wrong using aiphyllium.
But some variations, south also has occurred in taking place frequently with global warming and EI Nino phenomenon, southern and northern weather
Side also will appear the larger snowfall of intensity in area winter.Such as the ground winter such as Anhui, Jiangsu, once bust heavy snow, accumulated snow will be in urban districts
The evergreen nursery stock be collapseds such as a large amount of cinnamomum camphora, wide yulans are broken, and cause huge economic loss.Currently, hindering for the nursery stock being pressed down
Mouth processing gardens department is generally carried out by the way of wound curing agent smearing, and this medicament and processing method are opposite in warm season
It is useful, but in winter use when medicament be easy to freeze, cannot effectively penetrate into inside plant tissues, callus effect is poor, and
And it is also not good enough for the Wound treatment effect of large area, callus processing is bad to may result in trees wound sense after temperature gos up
Dye is dead, causes the economic loss of bigger.
Invention content
In view of the above problems, the purpose of the present invention is to provide a kind of low temperature season evergreen tree with callus coat material and its
Processing method, the medicament and processing method during the cold season under still can play good trees callus effect, and can be with
Wound surface treatment suitable for larger area.
A kind of low temperature season evergreen tree callus cladding material, the preparation method of callus cladding material are as follows：
(1) prepare following raw material according to mass fraction：5-6 parts of seaweeds, 10-13 parts of brassin, -5 parts of Catergen, mountain
4-8 parts of potassium sorbate, 1-3 parts of potassium iodide, 3-4 parts of chitosan oligosaccharide citrate, 0.1-0.3 parts of plant hormone lysate, bacteria remover 2-4
Part, 50-60 parts of glucose solution.
(2) seaweeds, brassin, vitamin C, potassium sorbate, potassium iodide, chitosan oligosaccharide citrate, bacteria remover are distinguished
It is added in glucose solution, is configured to callus agent solution, is stirred under 30-35 DEG C of bath temperature after the completion of wound curing agent solution allocation
It mixes and handles 5-10min, it is spare.
(3) hospital gauze is taken, it is 2.5-3cm that hospital gauze, which is laminated into nature thickness, then uses gauze surrounding
Sterile line encapsulation, is made cladding gauze, cladding gauze is immersed in callus agent solution, gauze to be covered takes out after soaking completely
It drains, sprays plant hormone lysate in cladding gauze surface after draining, the amount of spraying is 0.3-0.6g/100c ㎡.
(4) after spray on plants hormone lysate, required callus cladding material is obtained, callus cladding material is close with medical aseptic bag
Envelope preserves.
Preferably, the plant hormone lysate in step (1) is that gibberellin, heteroauxin, the ethyl alcohol of the basic element of cell division are molten
The concentration of liquid, three is respectively 200ppm, 150ppm and 200ppm.
Preferably, streptomysin, thiophanate methyl and difenoconazole are contained in the bacteria remover in step (1), three's contains
Amount is than being 4:3:3.
Preferably, a concentration of 5% of the glucose solution in step (1).
Preferably, the pH value of the callus agent solution in step (2) is 6.3-6.5.
Preferably, it is preserved under room temperature state after callus cladding material packaging in step (4), the term of validity is 12-15 months.
A kind of callus processing method using above-mentioned callus cladding material, including following processing step：The disconnected branch of trees is cut
Remove, wound surface handled, with clear water by wound irrigation it is clean after, wound bark is scraped off with sharp sword, expose bast
Portion, cutting bast keep wound flat smooth, then will be coated on wound after the 5% glucose solution wetting of callus cladding material
Discharge surface keeps exposed bast completely to be covered by callus cladding material, then fixes callus packet with medical bandage winding wound
Cover material, finally wound trunk is completely wound with gas permeability tarpaulin or straw rope carry out it is warming, using can be by bandage after three months
It is removed with cladding material.
A kind of low temperature season evergreen tree provided by the invention coats material and its processing method with callus, with prior art phase
Than haing the following advantages：
Contain streptomysin, thiophanate methyl and difenoconazole three in the callus agent solution used in type callus cladding material
Kind degerming component, can prevent trauma surface infestation after use, a variety of by seaweeds, brassin, vitamin C and potassium sorbate etc.
Substance promotes Wound healing, wherein uses the plant accurately configured to swash in the plant hormone lysate used in callus cladding material
Element promotes the wound tissue fast-growth of trees in cold season, further promotes using plant hormone coordinate plant growth
Wound healing.
The various composition of type callus cladding material is dissolved with glucose solution, on the one hand can be reduced to plant cell
Stimulation, be conducive to plant tissue and cell and absorb, on the other hand since glucose solution freezing point is relatively low, medicament can be made to have
Better cryoprotective effects prevent the main component of wound curing agent is frozen cannot be efficiently absorbed, it is difficult to play a role.Use cladding
Carrier of the gauze as medicament, on the one hand absorbs medicament convenient for plant, on the other hand can reduce the damage to the surface of a wound,
And the surface of a wound can be protected, moreover it is possible to provide cold-resistant warming effect for the surface of a wound, promote cell division and Wound healing.
The use of type callus cladding material and processing method need to remove on the bark of wound area, then directly by callus
Cladding material is coated on plant phloem tissue, this makes can quickly being contacted with plant living body cell for callus agent solution, from
And growth and the differentiation mechanism of plant cell are directly adjusted, fast onset callus effect.After having handled, callus cladding material and bandage
Straw rope or gas permeability tarpaulin are also wound outside, ensure to improve heat insulation effect while surface of a wound gas permeability.
Specific implementation mode
The specific implementation mode of the present invention is described in detail below.It should be understood that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to restrict the invention.
Embodiment 1
A kind of low temperature season evergreen tree callus cladding material, the preparation method of callus cladding material are as follows：
(1) prepare following raw material according to mass fraction：5 parts of seaweeds, 10 parts of brassin, Catergen part, potassium sorbate 4
Part, 1 part of potassium iodide, 3 parts of chitosan oligosaccharide citrate, 0.1 part of plant hormone lysate, 2 parts of bacteria remover, 50 parts of glucose solution.
(2) seaweeds, brassin, vitamin C, potassium sorbate, potassium iodide, chitosan oligosaccharide citrate, bacteria remover are distinguished
It is added in glucose solution, is configured to callus agent solution, is stirred under 30-35 DEG C of bath temperature after the completion of wound curing agent solution allocation
It mixes and handles 5min, it is spare.
(3) hospital gauze is taken, it is 2.5cm that hospital gauze, which is laminated into nature thickness, then by gauze surrounding nothing
Bacterium line encapsulates, and cladding gauze is made, and cladding gauze is immersed in callus agent solution, and gauze to be covered takes out drip after soaking completely
It is dry, plant hormone lysate is sprayed in cladding gauze surface after draining, the amount of spraying is 0.3g/100c ㎡.
(4) after spray on plants hormone lysate, required callus cladding material is obtained, callus cladding material is close with medical aseptic bag
Envelope preserves.
Wherein, the plant hormone lysate in step (1) is the ethanol solution of gibberellin, heteroauxin, the basic element of cell division,
The concentration of three is respectively 200ppm, 150ppm and 200ppm；Contain streptomysin, thiophanate methyl and phenyl ether methyl cyclic in bacteria remover
The content ratio of azoles, three is 4:3:3；A concentration of the 5% of glucose solution.；The pH value of callus agent solution in step (2) is
6.3.It is preserved under room temperature state after callus cladding material packaging in step (4), the term of validity is 12 months.
A kind of callus processing method using above-mentioned callus cladding material, including following processing step：The disconnected branch of trees is cut
Remove, wound surface handled, with clear water by wound irrigation it is clean after, wound bark is scraped off with sharp sword, expose bast
Portion, cutting bast keep wound flat smooth, then will be coated on wound after the 5% glucose solution wetting of callus cladding material
Discharge surface keeps exposed bast completely to be covered by callus cladding material, then fixes callus packet with medical bandage winding wound
Cover material, finally wound trunk is completely wound with gas permeability tarpaulin or straw rope carry out it is warming, using can be by bandage after three months
It is removed with cladding material.
Embodiment 2
A kind of low temperature season evergreen tree callus cladding material, the preparation method of callus cladding material are as follows：
(1) prepare following raw material according to mass fraction：6 parts of seaweeds, 13 parts of brassin, 5 parts of vitamin C, potassium sorbate 8
Part, 3 parts of potassium iodide, 4 parts of chitosan oligosaccharide citrate, 0.3 part of plant hormone lysate, 4 parts of bacteria remover, 60 parts of glucose solution.
(2) seaweeds, brassin, vitamin C, potassium sorbate, potassium iodide, chitosan oligosaccharide citrate, bacteria remover are distinguished
It is added in glucose solution, is configured to callus agent solution, is stirred under 35 DEG C of bath temperatures after the completion of wound curing agent solution allocation
10min is handled, it is spare.
(3) hospital gauze is taken, it is 3cm that hospital gauze, which is laminated into nature thickness, then by gauze surrounding with sterile
Line encapsulates, and cladding gauze is made, and cladding gauze is immersed in callus agent solution, and gauze to be covered takes out drip after soaking completely
It is dry, plant hormone lysate is sprayed in cladding gauze surface after draining, the amount of spraying is 0.6g/100c ㎡.
(4) after spray on plants hormone lysate, required callus cladding material is obtained, callus cladding material is close with medical aseptic bag
Envelope preserves.
Wherein, the plant hormone lysate in step (1) is the ethanol solution of gibberellin, heteroauxin, the basic element of cell division,
The concentration of three is respectively 200ppm, 150ppm and 200ppm；Contain streptomysin, thiophanate methyl and phenyl ether methyl cyclic in bacteria remover
The content ratio of azoles, three is 4:3:3；A concentration of the 5% of glucose solution.；The pH value of callus agent solution in step (2) is
6.5.It is preserved under room temperature state after callus cladding material packaging in step (4), the term of validity is 15 months.
A kind of callus processing method using above-mentioned callus cladding material, including following processing step：The disconnected branch of trees is cut
Remove, wound surface handled, with clear water by wound irrigation it is clean after, wound bark is scraped off with sharp sword, expose bast
Portion, cutting bast keep wound flat smooth, then will be coated on wound after the 5% glucose solution wetting of callus cladding material
Discharge surface keeps exposed bast completely to be covered by callus cladding material, then fixes callus packet with medical bandage winding wound
Cover material, finally wound trunk is completely wound with gas permeability tarpaulin or straw rope carry out it is warming, using can be by bandage after three months
It is removed with cladding material.
Embodiment 3
A kind of low temperature season evergreen tree callus cladding material, the preparation method of callus cladding material are as follows：
(1) prepare following raw material according to mass fraction：5.5 parts of seaweeds, 12 parts of brassin, 3.5 parts of vitamin C, sorb
6 parts of sour potassium, 2 parts of potassium iodide, 3.5 parts of chitosan oligosaccharide citrate, 0.2 part of plant hormone lysate, 3 parts of bacteria remover, glucose is molten
55 parts of liquid.
(2) seaweeds, brassin, vitamin C, potassium sorbate, potassium iodide, chitosan oligosaccharide citrate, bacteria remover are distinguished
It is added in glucose solution, is configured to callus agent solution, is stirred under 33 DEG C of bath temperatures after the completion of wound curing agent solution allocation
7min is handled, it is spare.
(3) hospital gauze is taken, it is 2.8cm that hospital gauze, which is laminated into nature thickness, then by gauze surrounding nothing
Bacterium line encapsulates, and cladding gauze is made, and cladding gauze is immersed in callus agent solution, and gauze to be covered takes out drip after soaking completely
It is dry, plant hormone lysate is sprayed in cladding gauze surface after draining, the amount of spraying is 0.5g/100c ㎡.
(4) after spray on plants hormone lysate, required callus cladding material is obtained, callus cladding material is close with medical aseptic bag
Envelope preserves.
Wherein, the plant hormone lysate in step (1) is the ethanol solution of gibberellin, heteroauxin, the basic element of cell division,
The concentration of three is respectively 200ppm, 150ppm and 200ppm；Contain streptomysin, thiophanate methyl and phenyl ether methyl cyclic in bacteria remover
The content ratio of azoles, three is 4:3:3；A concentration of the 5% of glucose solution.；The pH value of callus agent solution in step (2) is
6.4.It is preserved under room temperature state after callus cladding material packaging in step (4), the term of validity is 14 months.
A kind of callus processing method using above-mentioned callus cladding material, including following processing step：The disconnected branch of trees is cut
Remove, wound surface handled, with clear water by wound irrigation it is clean after, wound bark is scraped off with sharp sword, expose bast
Portion, cutting bast keep wound flat smooth, then will be coated on wound after the 5% glucose solution wetting of callus cladding material
Discharge surface keeps exposed bast completely to be covered by callus cladding material, then fixes callus packet with medical bandage winding wound
Cover material, finally wound trunk is completely wound with gas permeability tarpaulin or straw rope carry out it is warming, using can be by bandage after three months
It is removed with cladding material.
Embodiment 4
A kind of low temperature season evergreen tree callus cladding material, the preparation method of callus cladding material are as follows：
(1) prepare following raw material according to mass fraction：5 parts of seaweeds, 13 parts of brassin, 3 parts of vitamin C, potassium sorbate 7
Part, 1 part of potassium iodide, 4 parts of chitosan oligosaccharide citrate, 0.1 part of plant hormone lysate, 3 parts of bacteria remover, 55 parts of glucose solution.
(2) seaweeds, brassin, vitamin C, potassium sorbate, potassium iodide, chitosan oligosaccharide citrate, bacteria remover are distinguished
It is added in glucose solution, is configured to callus agent solution, is stirred under 34 DEG C of bath temperatures after the completion of wound curing agent solution allocation
6min is handled, it is spare.
(3) hospital gauze is taken, it is 2.5cm that hospital gauze, which is laminated into nature thickness, then by gauze surrounding nothing
Bacterium line encapsulates, and cladding gauze is made, and cladding gauze is immersed in callus agent solution, and gauze to be covered takes out drip after soaking completely
It is dry, plant hormone lysate is sprayed in cladding gauze surface after draining, the amount of spraying is 0.4g/100c ㎡.
(4) after spray on plants hormone lysate, required callus cladding material is obtained, callus cladding material is close with medical aseptic bag
Envelope preserves.
Wherein, the plant hormone lysate in step (1) is the ethanol solution of gibberellin, heteroauxin, the basic element of cell division,
The concentration of three is respectively 200ppm, 150ppm and 200ppm；Contain streptomysin, thiophanate methyl and phenyl ether methyl cyclic in bacteria remover
The content ratio of azoles, three is 4:3:3；A concentration of the 5% of glucose solution.；The pH value of callus agent solution in step (2) is
6.3.It is preserved under room temperature state after callus cladding material packaging in step (4), the term of validity is 12 months.
A kind of callus processing method using above-mentioned callus cladding material, including following processing step：The disconnected branch of trees is cut
Remove, wound surface handled, with clear water by wound irrigation it is clean after, wound bark is scraped off with sharp sword, expose bast
Portion, cutting bast keep wound flat smooth, then will be coated on wound after the 5% glucose solution wetting of callus cladding material
Discharge surface keeps exposed bast completely to be covered by callus cladding material, then fixes callus packet with medical bandage winding wound
Cover material, finally wound trunk is completely wound with gas permeability tarpaulin or straw rope carry out it is warming, using can be by bandage after three months
It is removed with cladding material.
Performance test
By experimental verification, the callus cladding material and callus processing method of the present embodiment are in processing cinnamomum camphora, wide yulan, osmanthus
When the aiphylliums winter major trauma faces such as tree, great Ye banyans, Wound healing rate reaches 100%, and callus effect is very outstanding, should be more
After wound cladding material is highly suitable for winter snow disaster, the callus of the wounds such as disconnected branch of aiphyllium uses.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited in above-mentioned
Particular implementation, those skilled in the art can make various deformations or amendments within the scope of the claims, this not shadow
Ring the substantive content of the present invention.
Claims (7)
- Expect 1. a kind of low temperature season evergreen tree is coated with callus, it is characterised in that：The preparation method of the callus cladding material is such as Under：（1）Prepare following raw material according to mass fraction：5-6 parts of seaweeds, 10-13 parts of brassin, 2-5 parts of vitamin C, sorb Sour potassium 4-8 parts, 1-3 parts of potassium iodide, 3-4 parts of chitosan oligosaccharide citrate, 0.1-0.3 parts of plant hormone lysate, bacteria remover 2-4 Part, 50-60 parts of glucose solution；（2）Seaweeds, brassin, vitamin C, potassium sorbate, potassium iodide, chitosan oligosaccharide citrate, bacteria remover are separately added into Into glucose solution, it is configured to callus agent solution, wound curing agent solution allocation is after the completion under 30-35 DEG C of bath temperature at stirring 5-10min is managed, it is spare；（3）Hospital gauze is taken, it is 2.5-3cm that hospital gauze, which is laminated into nature thickness, then by gauze surrounding with sterile Line encapsulates, and cladding gauze is made, and cladding gauze is immersed in callus agent solution, and gauze to be covered takes out drip after soaking completely It is dry, plant hormone lysate is sprayed in cladding gauze surface after draining, the amount of spraying is 0.3-0.6g/100c ㎡；（4）After spray on plants hormone lysate, required callus cladding material is obtained, callus cladding material is sealed with medical aseptic bag and is protected It deposits.
- 2. low temperature season evergreen tree is coated with callus and is expected according to claim 1, it is characterised in that：Step（1）The plant Object hormone lysate is the ethanol solution of gibberellin, heteroauxin, the basic element of cell division, and the concentration of three is respectively 200ppm, 150ppm and 200ppm.
- 3. low temperature season evergreen tree is coated with callus and is expected according to claim 1, it is characterised in that：Step（1）It is described to remove Contain streptomysin, thiophanate methyl and difenoconazole in microbial inoculum, the content ratio of three is 4:3:3.
- 4. low temperature season evergreen tree is coated with callus and is expected according to claim 1, it is characterised in that：Step（1）The Portugal A concentration of the 5% of grape sugar juice.
- 5. low temperature season evergreen tree is coated with callus and is expected according to claim 1, it is characterised in that：Step（2）Described The pH value of callus agent solution is 6.3-6.5.
- 6. low temperature season evergreen tree is coated with callus and is expected according to claim 1, it is characterised in that：Step（4）It is described to be cured It is preserved under room temperature state after wound cladding material packaging, the term of validity is 12-15 months.
- 7. a kind of callus processing method using callus cladding material described in claim 1, it is characterised in that：Including handling step as follows Suddenly：The disconnected branches of trees is amputated, wound surface is handled, with clear water by wound irrigation it is clean after, wound bark is used Sharp sword scrapes off, and exposes bast, and cutting bast keeps wound flat smooth, then that callus cladding material is molten with 5% glucose It is coated on wound surface after liquid wetting, keeps exposed bast completely to be covered by callus cladding material, is then twined with medical bandage Callus cladding material is fixed around wound, finally wound trunk is completely wound with gas permeability tarpaulin or straw rope and carries out warming, use Bandage and cladding material can be removed after three months.
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| CN201810125218.8A CN108294051A (en) | 2018-02-08 | 2018-02-08 | A kind of low temperature season evergreen tree callus cladding material and its processing method |
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| CN201810125218.8A CN108294051A (en) | 2018-02-08 | 2018-02-08 | A kind of low temperature season evergreen tree callus cladding material and its processing method |
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| WO2021063007A1 (en) * | 2019-09-30 | 2021-04-08 | 枣庄学院 | Plaster used for repairing tree wound and preparation method and use thereof |
| CN113575151A (en) * | 2021-07-28 | 2021-11-02 | 东北林业大学 | Poplar cuttage stable rooting method |
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