WO2021063007A1 - Plaster used for repairing tree wound and preparation method and use thereof - Google Patents

Abstract

A preparation method for a plaster used for repairing a tree wound and a use method. The preparation method comprises: (1) first pre-dissolving gibberellin with ethanol to prepare a 10 mg/ml solution; (2) preparing 20-40 g/L of cyclodextrin, 15-30 g/L of sodium carboxymethyl cellulose, and 30-50 g/L of purple sweet potato powder, and fully and uniformly mixing the three components; (3) adding the gibberellin ethanol solution obtained in step (1) with 1-5 mg/L of gibberellin into clean tap water; (4) sequentially adding 100 mg/L of cytokinin, 0.5-1 g/L of carbendazim, and 3-5 g/L of potassium sorbate into step (3), and fully and uniformly stirring to obtain a mixed solution; (5) adding the mixed powder obtained in step (2) into the mixed solution obtained in step (4), and uniformly stirring until the mixture is sufficiently dissolved to obtain the plaster. The use method for the plaster comprises: the prepared plaster is separately packed with "a toothpaste tube" having a capacity of 200-500 g; a cap is unscrewed when the plaster is used, and the plaster is squeezed out and applied on a new wound of the trunk; then, the plaster is wrapped with a preservative film for moisture preservation; the plaster is generally applied once on a wound of less than 3 cm; the plaster can be applied repeatedly on a large wound which is incompletely healed within one month. The plaster can shorten wound healing time.

Description

A plaster for repairing tree wounds and its preparation method and application Technical field

The invention belongs to the technical field of plant cultivation environmental condition adjustment, and relates to the preparation and application of a plant wound repair plaster, which is applied to the repair of branch and stem damage of fruit trees and green seedlings, accelerates the speed of plant healing, and reduces the degree of damage to trees.

Background technique

During the growth of the tree, it will suffer mechanical damage from various aspects, such as pruning, bruising, moth-eaten, etc., and the damage formed on the branches of the tree. The lighter ones will cause poor plant growth, produce tree tumors, and affect the quality of timber trees. , Reduce the yield and product quality of fruit trees, the more serious ones will cause the trees to die. Trees such as locust trees, willows and other trees will form protruding tree bumps on the trunk after the bark of trees such as locust trees and willows are damaged. The tree bumps are ugly in shape and hard in texture, which will cause plants to grow distorted and destroy their original wood structure, making it difficult to process into timber. . After the platanus tree trunk is injured, if it cannot heal quickly, the xylem will be exposed, and the damaged part will begin to decay, forming a hollow trunk. When peach trees, grapes and other fruit trees are injured, they secrete mucus, which loses nutrients in the tree and reduces yield.

Gibberellin is a widely existing plant hormone. The chemical structure belongs to diterpenoid acids, derived from the four-ring skeleton. There are at least 38 kinds of gibberellins, which are used in agricultural production to stimulate the growth of leaves and buds and increase yields.

Cytokinin (CTK) is a plant hormone isolated or artificially synthesized from corn or other plants. Generally produced in plant roots, it is a kind of substance that promotes cytokinesis and promotes the differentiation and growth of various tissues. The natural cytokinins in plants include zeatin (ZT), dihydrozeatin, isopentenyl adenine, zeatin nucleoside, and isopentenyl adenosine. The main part of their synthesis in the body is the root tip. In addition to kinetin, synthetic cytokinins also include 6-benzylaminopurine (6-BA) and so on.

Carbendazim is also known as Mianweiling and Benzimidazole No. 44. Carbendazim is a broad-spectrum fungicide, which has the effect of preventing and curing diseases caused by fungi (such as deuteromycetes and polyascus) in many crops.

Potassium sorbate is a food preservative with broad-spectrum bactericidal properties, and because it is easily available at relatively low prices, it has become the most preferred food preservative of many food companies.

Cyclodextrin complexes exist in nature and can also be artificially synthesized. In industry, many dyes use cyclodextrin as the matrix; and many medicinal plants with medical effects, such as aloe, contain cyclodextrin complexes. For example, the cyclodextrin complex in the gel of aloe vera has anti-inflammatory, anti-swelling, analgesic, anti-itch and anti-bacterial effects, and can be used as a natural healing medicine. Often used as a thickener and excipient.

Sodium carboxymethyl cellulose is the most widely used and most used cellulose thickener and excipient in the world today.

technical problem

After the trunk of a tree is traumatized, under normal circumstances, just leave it alone and do nothing. Sometimes a simple method is adopted. The trunk of some trees is wrapped with a film, and some are painted on the wound surface to protect it, but The healing rate is slower. At present, there is no public report about the special plaster that promotes the repair of tree wounds.

Technical solutions

In view of the shortcomings of the prior art, the present invention provides a plant wound repair plaster that is pollution-free, non-polluting, and biosafety, which is easy to use, and accelerates the speed of repair and healing of the wound.

A plaster for repairing tree wounds, which is characterized in that it comprises the following steps: (1) Gibberellin is pre-dissolved with ethanol and prepared into a 10mg/mL solution for use; (2) Cyclodextrin 20-40g/ L. Sodium carboxymethyl cellulose 15-30g/L, purple potato powder 30-50g/L. Mix the three powders thoroughly, and set aside; (3) According to the ratio of gibberellin 1-5mg/L, the steps ( 1) Add the obtained gibberellin ethanol solution to clean tap water; (4) Add the three in order according to the ratio of cytokinin 100mg/L, carbendazim 0.5-1g/L, and potassium sorbate 3-5g/L In step (3), stir thoroughly to obtain a mixed solution; (5) add the mixed powder obtained in (2) to the mixed solution obtained in step (4), stir evenly until fully dissolved, and obtain a thick and uniform plaster product.

A plaster for repairing tree wounds, which is characterized in that it comprises the following steps: (1) Gibberellin is pre-dissolved in ethanol and prepared into a 10mg/mL solution for use; (2) 30g/L cyclodextrin is prepared, Sodium carboxymethyl cellulose 20g/L and purple potato powder 40g/L. Mix the three powders thoroughly, and set aside; (3) According to the ratio of gibberellin 3mg/L, the gibberellin ethanol obtained in step (1) The solution is added to clean tap water; (4) According to the ratio of cytokinin 100mg/L, carbendazim 0.8g/L, and potassium sorbate 4g/L, add the three in step (3) in turn, and stir them evenly. Obtain a mixed solution; (5) Add the mixed powder obtained in (2) to the mixed solution obtained in step (4), stir evenly to fully dissolve, and obtain a thick and uniform plaster product.

A preparation method of plaster for repairing tree wounds, which is characterized by: (1) gibberellin is pre-dissolved in ethanol and prepared into a 10mg/mL solution for use; (2) cyclodextrin 20-40g/L is prepared , Carboxymethyl cellulose sodium 15-30g/L, purple potato powder 30-50g/L, mix the three powders thoroughly, and set aside; (3) According to the ratio of gibberellin 1-5mg/L, step (1 ) The obtained gibberellin ethanol solution is added to clean tap water; (4) The three are sequentially added to the step according to the ratio of cytokinin 100mg/L, carbendazim 0.5-1g/L, and potassium sorbate 3-5g/L (3) and stir well to obtain a mixed solution; (5) add the mixed powder obtained in (2) to the mixed solution obtained in step (4), stir evenly to fully dissolve, and obtain a thick and uniform plaster product .

The preparation method of plaster for repairing tree wounds is characterized by: (1) gibberellin is pre-dissolved in ethanol and prepared into a 10mg/mL solution for use; (2) 30g/L cyclodextrin and carboxymethyl are prepared Cellulose sodium 20g/L, purple potato powder 40g/L, mix the three powders thoroughly, and set aside; (3) Add the gibberellin ethanol solution obtained in step (1) to the cleaned gibberellin at a ratio of 3 mg/L gibberellin (4) According to the ratio of 100mg/L of cytokinin, 0.8g/L of carbendazim, and 4g/L of potassium sorbate, add the three in step (3), and stir well to obtain a mixture Solution; (5) Add the mixed powder obtained in (2) to the mixed solution obtained in step (4), stir evenly to fully dissolve, and obtain a thick and uniform plaster product.

An application of plaster for repairing tree wounds, which is characterized in that the prepared plaster is divided into 200-500g capacity "toothpaste tubes", and the cap is unscrewed during use, and then squeezed out to apply to the new wound on the trunk. Wrap it with plastic wrap and keep it moisturized. If the wound surface is within 3cm, apply it once; if the wound area is larger and does not fully heal within one month, apply it again. The effect is best in spring, summer, and autumn in the middle of the year.

Beneficial effect

The trunk wound repair plaster of the present invention can significantly shorten the wound healing time, and can shorten the wound healing time by more than 35%.

Embodiments of the present invention

The formula of trauma plaster:

water

Gibberellin 1-5mg/L

Cytokinin 100mg/L

Carbendazim 0.5-1g/L

Potassium Sorbate 3-5g/L

Cyclodextrin 20-40g/L

Sodium carboxymethyl cellulose 15-30g/L

Purple sweet potato flour 30-50g/L

Production method: (1) Gibberellin is pre-dissolved in ethanol and prepared as a 10mg/mL solution for later use; (2) According to cyclodextrin 20-40g/L, sodium carboxymethyl cellulose 15-30g/L, purple The ratio of 30-50g/L of potato flour is calculated for the dosage of the three, weighed separately, the powders of the three are fully mixed, and set aside; (3) According to the ratio of gibberellin 1-5mg/L, the amount obtained from (1) Add the gibberellin ethanol solution to clean tap water; (4) Add the three in order according to the ratio of cytokinin 100mg/L, carbendazim 0.5-1g/L, and potassium sorbate 3-5g/L, and fully Stir uniformly to obtain a mixed solution; (5) Add the mixed powder obtained in (3) to the mixed solution obtained in step (4), stir evenly until fully dissolved, and obtain a thick and uniform plaster product.

Packaging and use:

The prepared plaster is divided into 200-500g capacity "toothpaste tubes". Unscrew the lid when using, squeeze out and apply to the new wound of the trunk, and then wrap the plaster with plastic wrap for moisturizing. If the wound surface is within 3cm, generally apply once. Yes; if the wound area is larger and does not heal completely within one month, you can apply again. The best results are in spring, summer and autumn in the middle of the year.

The water used in the present invention can be clean tap water; the gibberellins and cytokinins are plant hormones, which can promote cell division and growth, and are the main components of the plaster to promote wound healing; carbendazim is a bactericide, which can promote cell division and growth. Kill bacteria and molds in wounds and prevent the wounds from rot; potassium sorbate is a bacteriostatic agent, which can prevent the plaster from deteriorating during storage and use, that is, it has an antiseptic effect; cyclodextrin, carboxymethyl cellulose Sodium has strong water absorption, increases the viscosity of the plaster, plays a role in water retention, and helps the plaster adhere to the surface of the wound of the trunk; in addition to increasing the viscosity, the purple potato powder also makes the plaster appear red, which serves as a marking function. . Except for water, other formula ingredients are commercially available.

Invention effect

The trunk wound repair plaster of the present invention can significantly shorten the wound healing time, and can shorten the wound healing time by more than 35%.

Comparative test: select 5-year-old apple trees, 8-year-old jujube trees, and 6-year-old paulownia trees. The number of samples is 30. The trunk bark girdling test was carried out in mid-April. The width of the girdling was 3cm. After girdling, the plaster of the present invention was used. Apply the wound to the wound and wrap it with plastic wrap. The control group was only wrapped with plastic wrap after girdling. Record the number of days required for the girdling wound to heal. The specific data are shown in Table 1 below.

Table 1. Comparison test results of wound healing of tree branches and stems

species	Treatment group/day	Control group/day	Compared with the control shortening ratio/%
apple	27±1.5	42±1.2	35.71
date	29±2.2	48±2.5	39.58
Fatong	23±1.8	36±2.1	36.11

Example

The application of the present invention will be further illustrated by the following examples, but it is not limited thereto.

Example 1

(1) Gibberellin is pre-dissolved in ethanol and prepared into a 10mg/mL solution for later use; (2) According to cyclodextrin 20-40g/L, carboxymethyl cellulose sodium 15-30g/L, purple potato powder 30 -50g/L ratio to calculate the dosage of the three, weigh them separately, mix the powders of the three thoroughly, and set aside; (3) According to the ratio of gibberellin 1-5mg/L, the gibberellin obtained in (1) Add the ethanol solution to clean tap water; (4) Add the three in sequence at the ratios of cytokinin 100mg/L, carbendazim 0.5-1g/L, and potassium sorbate 3-5g/L, and stir well. Obtain a mixed solution; (5) Add the mixed powder obtained in (3) to the mixed solution obtained in step (4), stir evenly to fully dissolve, and obtain a thick and uniform plaster product.

Example 2

(1) Pre-dissolve gibberellin with ethanol and prepare a 10mg/mL solution for later use; (2) Prepare cyclodextrin 30g/L, carboxymethyl cellulose sodium 20g/L, purple potato powder 40g/L (3) Add the gibberellin ethanol solution obtained in step (1) to clean tap water according to the ratio of gibberellin 3mg/L; (4) according to cytokinin 100mg/L, The ratio of carbendazim 0.8g/L and potassium sorbate 4g/L are added to step (3) in sequence, and stirred well to obtain a mixed solution; (5) add the mixed powder obtained in (2) to (4) In the mixed solution obtained in step 1), stir evenly until it is fully dissolved to obtain a thick and uniform plaster product.

Claims (5)

1. A plaster for repairing tree wounds, which is characterized in that it comprises the following steps:

   (1) Gibberellin is pre-dissolved in ethanol and prepared as a 10mg/mL solution for later use;

   (2) Prepare cyclodextrin 20-40g/L, sodium carboxymethyl cellulose 15-30g/L, and purple sweet potato powder 30-50g/L. Mix the three powders thoroughly, and set aside;

   (3) Add the gibberellin ethanol solution obtained in step (1) into clean tap water according to the ratio of gibberellin 1-5mg/L;

   (4) According to the ratio of 100mg/L of cytokinin, 0.5-1g/L of carbendazim, and 3-5g/L of potassium sorbate, add the three in step (3), and stir well to obtain a mixed solution. ；

   (5) Add the mixed powder obtained in (2) to the mixed solution obtained in step (4), stir evenly to fully dissolve, and obtain a thick and uniform plaster product.
2. The plaster for repairing tree wounds according to claim 1, characterized in that it comprises the following steps:

   (1) Gibberellin is pre-dissolved in ethanol and prepared as a 10mg/mL solution for later use;

   (2) Prepare 30g/L of cyclodextrin, 20g/L of sodium carboxymethylcellulose, 40g/L of purple potato powder, mix the three powders thoroughly, and set aside;

   (3) Add the gibberellin ethanol solution obtained in step (1) to clean tap water according to the ratio of gibberellin 3mg/L;

   (4) According to the ratio of cytokinin 100mg/L, carbendazim 0.8g/L, and potassium sorbate 4g/L, add the three in step (3) in sequence, and stir well to obtain a mixed solution;

   (5) Add the mixed powder obtained in (2) to the mixed solution obtained in step (4), stir evenly to fully dissolve, and obtain a thick and uniform plaster product.
3. A preparation method of plaster for repairing tree wounds according to claim 1 or 2, characterized in that:

   (1) Gibberellin is pre-dissolved in ethanol and prepared as a 10mg/mL solution for later use;

   (2) Prepare cyclodextrin 20-40g/L, sodium carboxymethyl cellulose 15-30g/L, and purple sweet potato powder 30-50g/L. Mix the three powders thoroughly, and set aside;

   (3) Add the gibberellin ethanol solution obtained in step (1) into clean tap water according to the ratio of gibberellin 1-5mg/L;

   (4) According to the ratio of 100mg/L of cytokinin, 0.5-1g/L of carbendazim, and 3-5g/L of potassium sorbate, add the three in step (3), and stir well to obtain a mixed solution. ；

   (5) Add the mixed powder obtained in (2) to the mixed solution obtained in step (4), stir evenly to fully dissolve, and obtain a thick and uniform plaster product.
4. The preparation method of plaster for repairing tree wounds according to claim 3, characterized in that:

   (1) Gibberellin is pre-dissolved in ethanol and prepared as a 10mg/mL solution for later use;

   (2) Prepare 30g/L of cyclodextrin, 20g/L of sodium carboxymethylcellulose, 40g/L of purple potato powder, mix the three powders thoroughly, and set aside;

   (3) Add the gibberellin ethanol solution obtained in step (1) to clean tap water according to the ratio of gibberellin 3mg/L;

   (4) According to the ratio of cytokinin 100mg/L, carbendazim 0.8g/L, and potassium sorbate 4g/L, add the three in step (3) in sequence, and stir well to obtain a mixed solution;

   (5) Add the mixed powder obtained in (2) to the mixed solution obtained in step (4), stir evenly to fully dissolve, and obtain a thick and uniform plaster product.
5. An application of a plaster for repairing tree wounds according to rights 1 to 4, which is characterized in that the prepared plaster is divided into a "toothpaste tube" with a capacity of 200-500g, and the lid is unscrewed and squeezed out during use. Apply to the new wound of the tree trunk, and wrap the plaster with a plastic wrap for moisturizing. If the wound is within 3cm, apply it once; if the wound area is larger and does not heal completely within one month, apply again, in the spring and summer of one year. , Autumn three seasons have the best effect.