CN106719746A - Plant of Lagerstroemia graft union growth regulator and preparation method thereof - Google Patents
Plant of Lagerstroemia graft union growth regulator and preparation method thereof Download PDFInfo
- Publication number
- CN106719746A CN106719746A CN201710023600.3A CN201710023600A CN106719746A CN 106719746 A CN106719746 A CN 106719746A CN 201710023600 A CN201710023600 A CN 201710023600A CN 106719746 A CN106719746 A CN 106719746A
- Authority
- CN
- China
- Prior art keywords
- parts
- plant
- lagerstroemia
- growth regulator
- graft union
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/18—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, directly attached to a heterocyclic or cycloaliphatic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
Abstract
The invention discloses a kind of plant of Lagerstroemia graft union growth regulator and preparation method thereof, it is made up of 1.50~3.50 parts of polyvinyl alcohol, 0.10~0.30 part of 6 benzyladenine, 0.05~0.10 part of gibberellin, 0.04~0.06 part of methyl α-naphthyl acetate, 2.50~4.50 parts of carbendazim, 0.20~0.30 part of pyridoxol, 3~5 parts of sucrose, 100~120 parts of absolute ethyl alcohol, 20~25 parts of distilled water.Plant graft wound healing can be promoted, graft survival rate and grafting quality is improved, with low production cost, preparation is simple, consumption is few, effect is good, remarkable in economical benefits effect.
Description
Technical field
The invention belongs to plant growth regulator technical field, be related to a kind of plant of Lagerstroemia graft union growth regulator and its
With Preparation Method.
Background technology
Grafting is a kind of breeding side that improvement seeds are reached using the advantageous feature of stock and the merit of scion variety
Method.In recent years, with the fast development of garden craft, grafting is more and more universal, and grafting method has been presented variation, diversification.
Can not only be promoted by grafting the winter resistance of plant, drought resistance, the ability of disease and insect resistance and ahead of time flowers bloom, fruit tree result
And function and the effect of improvement fruit quality.Meanwhile, on ornamental plant plastic arts, many can also be produced by grafting
Newly, plant very, ingeniously, beautiful pricks scape image, improves artistry, appreciative and the sight of gardens product, meets consumers in general
Diversified demand.
The factor of grafting survival and grafting quality is influenceed in production practices in addition to anvil fringe affinity, also with the nutrition of anvil fringe
The factors such as situation, moisture, sterilization are relevant.In order to ensure the survival rate of grafting, people apply to grafting using various chemical substances
To promote grafting wound to heal as early as possible, there is certain effect.
At present, plant consolidant used in production application, such as《A kind of preparation method of plant graft consolidant》(patent
Number：ZL201210096796.6, publication date：On August 15th, 2012), it is applied to around graft union when using, reach promotion
Grafting wound healing improves the effect of the survival rate of grafting.But due to the reasons such as weather and human users, general graft survival rate
Only 65% or so, survival rate is not high.Meanwhile, this plant consolidant is to shortening the plant graft cycle, promoting plant to increase
The aspect effect such as thicker is not obvious.《Tree wound healing protective agent and preparation method thereof》(the patent No.：
ZL201110202358.9, publication date：On 2 1st, 2012), trees, fruit tree are applied to when using and cut saw kerf, wound and corruption
In rotten sick scar, reach sterilization, controlling disease, promote wound healing, effect is obvious.But the trees consolidant is in plant graft
On do not carry out Practical experiments, if can improve grafting trees survival rate and grafting quality needs further inspection.
Therefore, current plant consolidant has following defect：1) sterilization, sterilized, antiseptic effect are poor, and wound is invaded by germ
Dye is serious, and healing time is more long；2) moisture and nutrient loss or insufficient supply in grafting procedures, unfavorable to plant growth；3) transfer
Interface wound face can not heal comprehensively, easily cause pest and disease damage to invade and cause the second subinfection, cause partial decomposition dry and decayed, and shadow
Ring tree body attractive in appearance；4) because failing timely and effectively to promote plant metabolism, accelerating cell division, shortening cell division after grafting
In the cycle, to cause plant graft excessive cycle, to increase thicker ability too slow；5) use of consolidant uses smearing mode, this
Plant mode practical operation difficulty big, grafting can be caused slow, have a strong impact on grafting efficiency.
Found by retrieving, used when there is no a grafting specifically designed for plant of Lagerstroemia in current existing consolidant
Healing growth regulator, and existing consolidant is difficult to fully meet the requirement of plant of Lagerstroemia grafting.Therefore, exploitation one kind is applied to
The healing growth regulator used during plant of Lagerstroemia grafting is to improve plant of Lagerstroemia seeds to transfer Jie survival rates and grafting quality urgently
The problem that need to be solved.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of plant of Lagerstroemia graft union growth regulator and its
Preparation method, substantially increases and transfers Jie survival rates and grafting quality.
The technical solution adopted in the present invention is, a kind of plant of Lagerstroemia graft union growth regulator, according to quality parts ratio,
It is formulated by following raw material：1.50~3.50 parts of polyvinyl alcohol, 0.10~0.30 part of 6-benzyladenine, gibberellin 0.05~
0.10 part, 0.04~0.06 part of methyl α-naphthyl acetate, 2.50~4.50 parts of carbendazim, 0.20~0.30 part of pyridoxol, 3~5 parts of sucrose, nothing
100~120 parts of water-ethanol, 20~25 parts of distilled water.
Further, according to quality parts ratio, it is formulated by following raw material：2.5 parts of polyvinyl alcohol, 6-benzyladenine
0.2 part, 0.08 part of gibberellin, 0.05 part of methyl α-naphthyl acetate, 3.5 parts of carbendazim, 0.25 part of pyridoxol, 4 parts of sucrose, absolute ethyl alcohol 110
Part, 22 parts of distilled water.
Another technical scheme for being taken of the present invention is：A kind of preparation method of plant of Lagerstroemia graft union growth regulator,
Specifically follow the steps below：
Step 1, according to quality parts ratio, weighs：1.50~3.50 parts of polyvinyl alcohol, 6-benzyladenine 0.10~0.30
Part, 0.05~0.10 part of gibberellin, 0.04~0.06 part of methyl α-naphthyl acetate, 2.50~4.50 parts of carbendazim, pyridoxol 0.20~0.30
Part, 3~5 parts of sucrose, 100~120 parts of absolute ethyl alcohol, 20~25 parts of distilled water,
Step 2, in 1 normal atmosphere pressure, polyvinyl alcohol is slowly added into distilled water, stirring while adding, profit
Heated with steam, until polyvinyl alcohol is completely dissolved, obtain poly-vinyl alcohol solution；
Step 3, gibberellin, 6-benzyladenine, pyridoxol are well mixed with sucrose, are slowly added under stirring
Absolute ethyl alcohol, mixes, and after material is all dissolved, adds carbendazim and methyl α-naphthyl acetate continues to stir 20min~30min,
Mixed liquor is made, is then poured into poly-vinyl alcohol solution, be sufficiently mixed to dissolve and stir and be mixed, that is, be obtained.
Further, in the step 2, the control of mixer rotating speed is in 150~180r/min during stirring.
Further, in the step 2, steam heating and temperature control is at 85~95 DEG C.
Further, in the step 3, the control of mixer rotating speed is in 120~150r/min during stirring.
Compared with prior art, the present invention has advantages below：
(1) when the present invention is applied to plant of Lagerstroemia grafting, with sterilization, sterilized, extra-nutrition and reduction moisture evaporation
Etc. function, make sterilization, anti-corrosion, nutrition, moisture while in place, and then reach promotion plant graft wound healing, raising is transferred
It is connected into motility rate and grafting quality；
(2) when the present invention is applied to plant of Lagerstroemia grafting, plant metabolism can be effectively facilitated, accelerates cell point
Split, stimulate hyperplasia, shorten CDC, the wound face of plant of Lagerstroemia graft union is quickly healed comprehensively,
And can interrogate and increase thicker fastly, shorten healing cycle；
(3) when the present invention is applied to plant of Lagerstroemia grafting, its graft survival rate up to more than 95.3%, do sth. in advance by healing stage
2~3 months (4~5 months phases of normal healing), 59.3% is improve compared with without any growth stimulator survival rate, increase 66.7
~80.0%, thicker 88.3~112.0%；Compared with plant consolidant (CN102630681B) is applied, graft survival rate is carried
It is high by 39.0%, increase 57.3~66.0%, thicker 69.7~88.3%.
(4) present invention has low production cost, preparation is simple, consumption is few, effect is good, remarkable in economical benefits effect.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
The present invention provides a kind of plant of Lagerstroemia graft union growth regulator, according to quality parts ratio, is prepared by following raw material
Form：1.50~3.50 parts of polyvinyl alcohol, 0.10~0.30 part of 6-benzyladenine (6-BA), gibberellin (GA3) 0.05~
0.10 part, 0.04~0.06 part of methyl α-naphthyl acetate (NAA), 2.50~4.50 parts of carbendazim, 0.20~0.30 part of B6 (pyridoxol), sucrose
3~5 parts, 100~120 parts of absolute ethyl alcohol, 20~25 parts of distilled water.
Wherein, polyvinyl alcohol, Main Function is in wound to form very thin biomembrane and increase the osmosis of medicine, can
Prevent rain drop erosion, nutrient loss, improve sterilization, disease-resistant, anti-exposed xylem wood corruption ability, be a kind of conventional security into
Film.
The concentration of the poly-vinyl alcohol solution in long dose of this healing power-assisted is just have preferable adherence and film, favorably
In rain drop erosion, nutrient loss is prevented, the abilities such as sterilization, disease-resistant, anti-corruption are improved, strengthen the function of other materials.If dense
Height is spent, though its adherence is improved, film sexual function is poor, be unfavorable for the infiltration of material, influence the healing effect of plant；
If concentration is too low, its adherence and film are all reduced, and the healing effect of plant is worse.
6~benzyladenine (6-BA) is a plant growth regulators, is divided with hyperplasia, acceleration cell is stimulated
Split, promote plant rapidly to increase thicker grade and act on.
Gibberellin (GA3), white crystal is insoluble in water, is dissolved in ethanol.As plant growth regulator, with most strong
Activity, can accelerate cambial cell to divide, promote callus quickly heal, and can stratification, shoot and leaf growth and ahead of time open
Spend solid effect.
Methyl α-naphthyl acetate (NAA), can be effectively promoted graft union cell division, promote the differentiation of undifferentiated tissue, callus induction group
Hair-weaving is given birth to, and promotes the effect such as wound healing.
6-benzyladenine (6-BA), gibberellin (GA3) and methyl α-naphthyl acetate (NAA) as plant growth substance or auxin, its
Action principle is similar, and all with amphicheirality, during less than plant optimum concentration, its concentration facilitation to plant growth higher is more
Substantially；During higher than plant optimum concentration, its concentration facilitation to plant growth higher is not all that low concentration promotes substantially
High concentration suppresses.As can be seen here, suitable 6-benzyladenine (6-BA), gibberellin (GA3) and methyl α-naphthyl acetate (NAA) solution concentration
Play very important effect to plant growth, and 6-benzyladenine (6-BA), gibberellin (GA3) in long dose of this healing power-assisted
The requirement of this standard is right up to methyl α-naphthyl acetate (NAA) solution concentration, promotes the growth of plant.
Carbendazim is a kind of biocide mildewcide, can cut off microorganisms spreading, graft union is prevented by microorganism infection, while right
Graft union plays sterilization and anticorrosion, promotes the effect of wound healing, but with carbendazim concentration rising to the restraint of plant growth
Strengthen.Therefore, add the carbendazim of this paper contents more suitable in long dose of the power-assisted that heals.
B6 (pyridoxol), white or slightly yellow crystal powder is dissolved in water, ethanol, acetone, being rich in nutrition value,
Played an important role in growing process, growth, cell division, the process into flower and aging of plant can be influenceed.B6 (pyrroles
Tremble alcohol) be culture medium main constituents, the height of its concentration plays very important influence to plant growth.If B6
(pyridoxol) concentration is higher, suppresses the absorption of Plant To Nutrient liquid；If B6 (pyridoxol) concentration is relatively low, plant absorption nutrient solution mistake
It is few, all can not effectively stimulate cell division and promote Effects on Plant Growth.If B6 (pyridoxol) concentration is in suitable level
(0.1%~0.3%), is conducive to the absorption of Plant To Nutrient material and utilizes, so as to more effectively promote the growth of plant.
Sucrose belongs to nutritional agents, can accelerate Callus formation suberin, while can be provided enough for neonatal cell
Nutrient, promote callus quickly heal.Sucrose concentration is relevant with the differentiation of xylem and bast, in the callus group of plant
When knitting culture, if sucrose concentration is relatively low in culture medium, by induced synthesis xylem；If sucrose concentration is higher, bast will be formed；
If sucrose concentration is formed in medium level (proportioning i.e. herein), induction xylem and bast simultaneously, and centre has
Forming layer, may advantageously facilitate plants wound and quickly heals.Sucrose also maintains the work of osmotic pressure in addition to the carbon source as culture
With.
A kind of preparation method of plant of Lagerstroemia graft union growth regulator, specifically follows the steps below：
Step 1, according to quality parts ratio, weighs：1.50~3.50 parts of polyvinyl alcohol, 6-benzyladenine (6-BA) 0.10
~0.30 part, 0.05~0.10 part of gibberellin (GA3), 0.04~0.06 part of methyl α-naphthyl acetate (NAA), 2.50~4.50 parts of carbendazim,
0.20~0.30 part of B6 (pyridoxol), 3~5 parts of sucrose, 100~120 parts of absolute ethyl alcohol, 20~25 parts of distilled water.
Step 2, in 1 normal atmosphere pressure, polyvinyl alcohol is slowly added into distilled water, stirring while adding, is stirred
The control of machine rotating speed is mixed in 150~180r/min, is heated using steam, temperature control is at 85~95 DEG C, until polyvinyl alcohol is complete
Dissolving, obtains poly-vinyl alcohol solution；
Step 3, gibberellin (GA3), 6-benzyladenine (6-BA), B6 (pyridoxol) are well mixed with sucrose, are being stirred
Mix and be slowly added to absolute ethyl alcohol under state, mix, mixer is stirred under the rotating speed of 120~150r/min, makes material complete
After portion's dissolving, add carbendazim and methyl α-naphthyl acetate (NAA) continues to stir 20min~30min, be made mixed liquor, then pour into poly-
In glycohol solution, it is sufficiently mixed to dissolve and stir and is mixed, be i.e. finished product plant of Lagerstroemia graft union growth regulator.
Step 4, by the bottling of finished product plant of Lagerstroemia graft union growth regulator, packaging.
Wherein, in whole preparation process, polyvinyl alcohol can only be dissolved in water, it is impossible to be dissolved in absolute ethyl alcohol；And sucrose,
B6 (pyridoxol) and methyl α-naphthyl acetate (NAA) are easily soluble in water and absolute ethyl alcohol；Carbendazim, gibberellin (GA3), 6-benzyladenine
(6-BA), is insoluble in water, dissolves in absolute ethyl alcohol.
The present invention is used for plant of Lagerstroemia because of the tree body wound caused by the clip produced by grafting, saw kerf and band girdle mouth,
Using can effectively protect wound after product of the present invention, prevent infection process, supplement the nutrients and reduce moisture evaporation, promote to plant
Thing metabolism, acceleration cell division, stimulation hyperplasia, shortening CDC, make the wound of plant of Lagerstroemia graft union
Hinder face quickly can heal comprehensively, and can interrogate and increase thicker fastly, shorten healing cycle, improve graft survival rate and grafting matter
Amount.When being applied to plant of Lagerstroemia grafting, with functions such as sterilization, sterilized, extra-nutrition and reduction moisture evaporations, meanwhile, this
Invention has low production cost, preparation is simple, consumption is few, effect is good, remarkable in economical benefits.
Embodiment 1
A kind of plant of Lagerstroemia graft union growth regulator, the composition of raw material is as follows with proportioning in technical scheme：Polyvinyl alcohol
1.50 grams, 0.1 gram of 6-benzyladenine (6-BA), 0.05 gram of gibberellin (GA3), 0.04 gram of methyl α-naphthyl acetate (NAA), carbendazim 2.5
Gram, 0.2 gram of B6 (pyridoxol), 3 grams of sucrose, 100 grams of absolute ethyl alcohol, 20 grams of distilled water.
A kind of preparation method of plant of Lagerstroemia graft union growth regulator, comprises the following steps that：In 1 standard atmospheric pressure
Under, polyvinyl alcohol is slowly added into distilled water, stirring while adding, mixer rotating speed is controlled in 150r/min, using steaming
Vapour is heated, and temperature control is at 85 DEG C, until polyvinyl alcohol is completely dissolved, obtains poly-vinyl alcohol solution.By gibberellin (GA3), 6-
Benzyladenine (6-BA), B6 (pyridoxol) are well mixed with sucrose, and absolute ethyl alcohol is slowly added under stirring, and mixing is stirred
Mix, mixer is stirred under the rotating speed of 120r/min, make material all dissolving after, add carbendazim and methyl α-naphthyl acetate (NAA) after
Continuous stirring 20min, is made mixed liquor.Then poly-vinyl alcohol solution is poured into, is sufficiently mixed to dissolve and stir and is mixed, be i.e. finished product
Plant of Lagerstroemia graft union growth regulator.
In order to verify the effect of present invention healing growth regulator, selection is with flourishing arrow as scion, 300 rivers Guizhou Province crape myrtle is brill wood
Grafting is carried out, wherein 100 graftings apply present invention healing growth regulator as experimental group 1, another 100 graftings are healed using plant
Used as experimental group 2, last 100 graftings do not apply any growth stimulator as sky for agent (using the patented technology of CN102630681B)
White control group, specific practice is：
Mid-March, when temperature on average is 20 DEG C or so, air relative temperature carries out Grafting Experiment when being 75%.It is right first
The blade of sterilization of experimental group 1 is pruned the crust and a little forming layer of grafting twig junction, it is desirable to which bevel is smooth；Use
The plant of Lagerstroemia graft union growth regulator that small sprayer will be prepared is sprayed to be cut on mouthful face, then wound is docked, and is pricked with cloth
Fasten.Experimental group 2 is pruned the crust and a little forming layer of grafting twig junction with the blade of sterilization, makes bevel smooth flat
It is whole, then wound is docked, the plant consolidant (CN102630681B) for preparing is coated, then fixation is tightened with cloth.Blank pair
Pruned the crust and a little forming layer of grafting twig junction with the blade of sterilization according to group, it is desirable to which bevel is smooth, then will wound
Mouth docking, fixation is tightened with cloth.
Result of the test is shown in Table 1
As seen from the above table, using present invention healing growth regulator, 30 days or so rivers Guizhou Province crape myrtle grafting incisional wound face is healed substantially
Close, the substantially all healing in crape myrtle grafting incisional wound face of 58 days or so rivers Guizhou Province, it is substantially all compared with experimental group 2 and blank control group to heal
Conjunction has been respectively shortened 7 days and 77 days；The graft survival rate of experimental group 1 will be transferred up to 95% apparently higher than experimental group 2 and blank control group
Work is connected into, 42% and 59% has been respectively increased；After grafting survival and wound heal completely, the graft union wound face light of experimental group 1
It is sliding, attractive in appearance, and it is thicker to be rised appreciably compared with experimental group 2 and blank control group grafting twig, and 50~60% and 60 are increased respectively
~75%, thicker 65~80% and 80~100%.Due to visible, river Guizhou Province crape myrtle can be accelerated by present invention healing growth regulator
The healing of wound, shortens the grafting cycle, improves graft survival rate, and can rapidly increase thicker, has compared with control group obvious
Growth vigor.
Embodiment 2
A kind of plant of Lagerstroemia graft union growth regulator, the composition of raw material is as follows with proportioning in technical scheme：Polyvinyl alcohol
2.50 grams, 0.2 gram of 6-benzyladenine (6-BA), 0.08 gram of gibberellin (GA3), 0.05 gram of methyl α-naphthyl acetate (NAA), carbendazim 3.50
Gram, 0.25 gram of B6 (pyridoxol), 4 grams of sucrose, 110 grams of absolute ethyl alcohol, 22 grams of distilled water.
A kind of preparation method of plant of Lagerstroemia graft union growth regulator, comprises the following steps that：In 1 standard atmospheric pressure
Under, polyvinyl alcohol is slowly added into distilled water, stirring while adding, mixer rotating speed is controlled in 180r/min, using steaming
Vapour is heated, and temperature control is at 95 DEG C, until polyvinyl alcohol is completely dissolved, obtains poly-vinyl alcohol solution.By gibberellin (GA3), 6-
Benzyladenine (6-BA), B6 (pyridoxol) are well mixed with sucrose, and absolute ethyl alcohol is slowly added under stirring, and mixing is stirred
Mix, mixer is stirred under the rotating speed of 150r/min, make material all dissolving after, add carbendazim and methyl α-naphthyl acetate (NAA) after
Continuous stirring 30min, is made mixed liquor.Then poly-vinyl alcohol solution is poured into, is sufficiently mixed to dissolve and stir and is mixed, be i.e. finished product
Plant of Lagerstroemia graft union growth regulator.
In order to verify the effect of present invention healing growth regulator, selection is with flourishing arrow as scion, 300 crape myrtles are carried out to bore wood
Grafting, wherein 100 graftings apply present invention healing growth regulator as experimental group 1, plant consolidant is applied in another 100 graftings
(CN102630681B) as experimental group 2, last 100 graftings do not apply any growth stimulator as blank control group,
Specific practice is：
Mid-March, when temperature on average is 20 DEG C or so, air relative temperature carries out Grafting Experiment when being 75%.It is right first
The blade of sterilization of experimental group 1 is pruned the crust and a little forming layer of grafting twig junction, it is desirable to which bevel is smooth；Use
The plant of Lagerstroemia graft union growth regulator that small sprayer will be prepared is sprayed to be cut on mouthful face, then wound is docked, and is pricked with cloth
Fasten.Experimental group 2 is pruned the crust and a little forming layer of grafting twig junction with the blade of sterilization, makes bevel smooth flat
It is whole, then wound is docked, the plant consolidant (CN102630681B) for preparing is coated, then fixation is tightened with cloth.Blank pair
Pruned the crust and a little forming layer of grafting twig junction with the blade of sterilization according to group, it is desirable to which bevel is smooth, then will wound
Mouth docking, fixation is tightened with cloth.
Result of the test is shown in Table 2
As seen from the above table, using present invention healing growth regulator, crape myrtle grafting incisional wound face is healed substantially within 25 days or so, and 52
It or so, the substantially all healing in crape myrtle grafting incisional wound face, healing substantially all compared with experimental group 2 and blank control group is contracted respectively
It is short 18 days and 88 days；The graft survival rate of experimental group 1 up to 98%, apparently higher than experimental group 2 and blank control group grafting survival,
It has been respectively increased 43% and 61%；After grafting survival and wound heal completely, the graft union wound face of experimental group 1 is smooth, attractive in appearance,
And it is thicker to be rised appreciably compared with experimental group 2 and blank control group grafting twig, 60~70% and 70~85% are increased respectively, become
Thick 77~96% and 90~120%.Due to visible, the healing of crape myrtle wound, contracting can be accelerated by present invention healing growth regulator
In the short grafting cycle, graft survival rate is improved, and can rapidly increase thicker, there is obvious growth vigor compared with control group.
Embodiment 3
A kind of plant of Lagerstroemia graft union growth regulator, the composition of raw material is as follows with proportioning in technical scheme：Polyvinyl alcohol
3.5 grams, 0.3 gram of 6~benzyladenine (6-BA), 0.10 gram of gibberellin (GA3), 0.06 gram of methyl α-naphthyl acetate (NAA), carbendazim 4.50
Gram, 0.30 gram of B6 (pyridoxol), 5 grams of sucrose, 120 grams of absolute ethyl alcohol, 25 grams of distilled water.
A kind of preparation method of plant of Lagerstroemia graft union growth regulator, comprises the following steps that：In 1 standard atmospheric pressure
Under, polyvinyl alcohol is slowly added into distilled water, stirring while adding, mixer rotating speed is controlled in 170r/min, using steaming
Vapour is heated, and temperature control is at 90 DEG C, until polyvinyl alcohol is completely dissolved, obtains poly-vinyl alcohol solution.By gibberellin (GA3), 6-
Benzyladenine (6-BA), B6 (pyridoxol) are well mixed with sucrose, and absolute ethyl alcohol is slowly added under stirring, and mixing is stirred
Mix, mixer is stirred under the rotating speed of 130r/min, make material all dissolving after, add carbendazim and methyl α-naphthyl acetate (NAA) after
Continuous stirring 25min, is made mixed liquor.Then poly-vinyl alcohol solution is poured into, is sufficiently mixed to dissolve and stir and is mixed, be i.e. finished product
Plant of Lagerstroemia graft union growth regulator.
In order to verify the effect of present invention healing growth regulator, selection is with flourishing arrow as scion, 300 Fujian crape myrtles are brill wood
Grafting is carried out, wherein 100 graftings apply present invention healing growth regulator as experimental group 1, another 100 graftings are healed using plant
Used as experimental group 2, last 100 graftings do not apply any growth stimulator as blank control group, specifically for agent (CN102630681B)
Way is：
Mid-March, when temperature on average is 20 DEG C or so, air relative temperature carries out Grafting Experiment when being 75%.It is right first
The blade of sterilization of experimental group 1 is pruned the crust and a little forming layer of grafting twig junction, it is desirable to which bevel is smooth；Use
The plant of Lagerstroemia graft union growth regulator that small sprayer will be prepared is sprayed to be cut on mouthful face, then wound is docked, and is pricked with cloth
Fasten.Experimental group 2 is pruned the crust and a little forming layer of grafting twig junction with the blade of sterilization, makes bevel smooth flat
It is whole, then wound is docked, the plant consolidant (CN102630681B) for preparing is coated, then fixation is tightened with cloth.Blank pair
Pruned the crust and a little forming layer of grafting twig junction with the blade of sterilization according to group, it is desirable to which bevel is smooth, then will wound
Mouth docking, fixation is tightened with cloth.
Result of the test is shown in Table 3
As seen from the above table, using present invention healing growth regulator, 31 days or so Fujian crape myrtle grafting incisional wound face is healed substantially
Close, 57 days or so, the Fujian substantially all healing in crape myrtle grafting incisional wound face was substantially all compared with experimental group 2 and blank control group
Healing has been respectively shortened 12 days and 83 days；The graft survival rate of experimental group 1, be apparently higher than experimental group 2 and blank up to 93%
Group grafting survival, has been respectively increased 32% and 58%；After grafting survival and wound heal completely, the graft union wound of experimental group 1
Face is smooth, attractive in appearance, and it is thicker to be rised appreciably compared with experimental group 2 and blank control group grafting twig, and 57~68% are increased respectively
With 65~80%, thicker 67~89% and 90~116%.Due to visible, Fujian can be accelerated by present invention healing growth regulator
The healing of crape myrtle wound, shortens the grafting cycle, improves graft survival rate, and can rapidly increase thicker, has compared with control group bright
Aobvious growth vigor.
By above-described embodiment as can be seen that plant of Lagerstroemia of the present invention heal growth regulator reasonable mixture ratio of components, to purple
Common vetch platymiscium grafting incisional wound face has good healing effect, with obvious growth vigor.Its graft survival rate reaches
More than 95.3%, healing stage has done sth. in advance 2~3 months (4~5 months phases of normal healing), compared with without any growth stimulator survival rate
59.3% is improve, 66.7~80.0% are increased, thicker 88.3~112.0%；With administration plant consolidant
(CN102630681B) compare, graft survival rate improves 39.0%, increase 57.3~66.0%, thicker 69.7~
88.3%.
Presently preferred embodiments of the present invention is the foregoing is only, is not intended to limit the scope of the present invention.Although
General explanation, specific embodiment and experiment are above used, the present invention is described in detail, but in base of the present invention
On plinth, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore, not inclined
The these modifications or improvements on the basis of spirit of the invention, belong to the scope of protection of present invention.
Claims (6)
1. a kind of plant of Lagerstroemia graft union growth regulator, it is characterised in that according to quality parts ratio, prepared by following raw material and
Into：1.50~3.50 parts of polyvinyl alcohol, 0.10~0.30 part of 6-benzyladenine, 0.05~0.10 part of gibberellin, methyl α-naphthyl acetate
0.04~0.06 part, 2.50~4.50 parts of carbendazim, 0.20~0.30 part of pyridoxol, 3~5 parts of sucrose, absolute ethyl alcohol 100~
120 parts, 20~25 parts of distilled water.
2. plant of Lagerstroemia graft union growth regulator according to claim 1, it is characterised in that according to quality parts ratio,
It is formulated by following raw material：2.5 parts of polyvinyl alcohol, 0.2 part of 6-benzyladenine, 0.08 part of gibberellin, 0.05 part of methyl α-naphthyl acetate,
3.5 parts of carbendazim, 0.25 part of pyridoxol, 4 parts of sucrose, 110 parts of absolute ethyl alcohol, 22 parts of distilled water.
3. a kind of preparation method of plant of Lagerstroemia graft union growth regulator, it is characterised in that specifically follow the steps below：
Step 1, according to quality parts ratio, weighs：1.50~3.50 parts of polyvinyl alcohol, 0.10~0.30 part of 6-benzyladenine,
0.05~0.10 part of gibberellin, 0.04~0.06 part of methyl α-naphthyl acetate, 2.50~4.50 parts of carbendazim, 0.20~0.30 part of pyridoxol,
3~5 parts of sucrose, 100~120 parts of absolute ethyl alcohol, 20~25 parts of distilled water,
Step 2, in 1 normal atmosphere pressure, polyvinyl alcohol is slowly added into distilled water, stirring while adding, using steaming
Vapour is heated, until polyvinyl alcohol is completely dissolved, obtains poly-vinyl alcohol solution；
Step 3, gibberellin, 6-benzyladenine, pyridoxol are well mixed with sucrose, are slowly added under stirring anhydrous
Ethanol, mixes, and after material is all dissolved, adds carbendazim and methyl α-naphthyl acetate continues to stir 20min~30min, is made
Mixed liquor, then pours into poly-vinyl alcohol solution, is sufficiently mixed to dissolve and stir and is mixed, that is, be obtained.
4. the preparation method of plant of Lagerstroemia graft union growth regulator according to claim 3, it is characterised in that the step
In rapid 2, the control of mixer rotating speed is in 150~180r/min during stirring.
5. the preparation method of plant of Lagerstroemia graft union growth regulator according to claim 3, it is characterised in that the step
In rapid 2, steam heating and temperature control is at 85~95 DEG C.
6. the preparation method of plant of Lagerstroemia graft union growth regulator according to claim 3, it is characterised in that the step
In rapid 3, the control of mixer rotating speed is in 120~150r/min during stirring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710023600.3A CN106719746A (en) | 2017-01-13 | 2017-01-13 | Plant of Lagerstroemia graft union growth regulator and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710023600.3A CN106719746A (en) | 2017-01-13 | 2017-01-13 | Plant of Lagerstroemia graft union growth regulator and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106719746A true CN106719746A (en) | 2017-05-31 |
Family
ID=58948118
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710023600.3A Pending CN106719746A (en) | 2017-01-13 | 2017-01-13 | Plant of Lagerstroemia graft union growth regulator and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106719746A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593137A (en) * | 2017-11-14 | 2018-01-19 | 万绿生态园林股份有限公司 | The method that crape myrtle cottage breeding is carried out using old stake |
| CN109362374A (en) * | 2018-11-09 | 2019-02-22 | 江苏省中国科学院植物研究所 | A method of improving color red poplar autumnbudding survival rate |
| CN111109287A (en) * | 2019-12-11 | 2020-05-08 | 山西省林业科学研究院 | Gleditsia sinensis grafting healing growth substance, preparation method and application |
| CN113892355A (en) * | 2021-09-17 | 2022-01-07 | 云南省农业科学院热带亚热带经济作物研究所 | Auxiliary agent for coffee grafting and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630681A (en) * | 2012-04-02 | 2012-08-15 | 何寒 | Preparation of plant graft healing agent and using method thereof |
| CN103518748A (en) * | 2013-09-24 | 2014-01-22 | 湖南紫薇投资集团有限公司 | Lagerstroemia indica tied-scene branch healing growth substance |
| CN104115661A (en) * | 2014-07-21 | 2014-10-29 | 江苏绿苑园林建设有限公司 | American lagerstroemia indica red rocket branch cutting propagation method |
| CN104521579A (en) * | 2014-12-30 | 2015-04-22 | 大连岭前农业专业合作社 | Cherry dense and dwarf planting method |
-
2017
- 2017-01-13 CN CN201710023600.3A patent/CN106719746A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630681A (en) * | 2012-04-02 | 2012-08-15 | 何寒 | Preparation of plant graft healing agent and using method thereof |
| CN103518748A (en) * | 2013-09-24 | 2014-01-22 | 湖南紫薇投资集团有限公司 | Lagerstroemia indica tied-scene branch healing growth substance |
| CN104115661A (en) * | 2014-07-21 | 2014-10-29 | 江苏绿苑园林建设有限公司 | American lagerstroemia indica red rocket branch cutting propagation method |
| CN104521579A (en) * | 2014-12-30 | 2015-04-22 | 大连岭前农业专业合作社 | Cherry dense and dwarf planting method |
Non-Patent Citations (2)
| Title |
|---|
| 于也: "提高嫁接成活率有新法", 《河北林业》 * |
| 张美勇 等: "《核桃安全生产技术指南》", 31 January 2012, 中国农业出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107593137A (en) * | 2017-11-14 | 2018-01-19 | 万绿生态园林股份有限公司 | The method that crape myrtle cottage breeding is carried out using old stake |
| CN109362374A (en) * | 2018-11-09 | 2019-02-22 | 江苏省中国科学院植物研究所 | A method of improving color red poplar autumnbudding survival rate |
| CN111109287A (en) * | 2019-12-11 | 2020-05-08 | 山西省林业科学研究院 | Gleditsia sinensis grafting healing growth substance, preparation method and application |
| CN111109287B (en) * | 2019-12-11 | 2021-08-27 | 山西省林业和草原科学研究院 | Gleditsia sinensis grafting healing growth substance, preparation method and application |
| CN113892355A (en) * | 2021-09-17 | 2022-01-07 | 云南省农业科学院热带亚热带经济作物研究所 | Auxiliary agent for coffee grafting and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2901661C (en) | High-energy composite peptide selenoprotein nutrient solution, preparation method and applications thereof | |
| CN106719746A (en) | Plant of Lagerstroemia graft union growth regulator and preparation method thereof | |
| CN110526770A (en) | A kind of tree grafting growth root-growing agent | |
| CN107258343A (en) | A kind of Chinese jujube high-efficiency engrafting and cultivating method | |
| AU2020102215A4 (en) | Foliar fertilizer rich in selenium (se) and calcium (ca) for rice plant and preparation method and use thereof | |
| CN106747927A (en) | A kind of popular ornamental trees and shrubs nutrient solution growth regulator and preparation method thereof | |
| CN102960149A (en) | Taxus chinensis cuttage cultivation method | |
| WO2021063007A1 (en) | Plaster used for repairing tree wound and preparation method and use thereof | |
| CN106069321A (en) | A kind of Fructus actinidiae chinensis cuttage and seedling culture method | |
| CN105850501A (en) | Method of planting Poria cocos using pine tree stumps | |
| CN104396968A (en) | Bark regenerant and preparation method and application thereof | |
| CN107098767A (en) | A kind of flower landscape composite cultivation nutrient solution and preparation method thereof | |
| CN106212184A (en) | A kind of method for transplanting of Fructus Vaccinii Bracteati seedling | |
| CN106631488A (en) | Composite fertilizer for potted anthurium | |
| CN106748362A (en) | A kind of method for planting dendrobium candidum in the tree | |
| CN106417350A (en) | Wound repairing agent for landscape plants | |
| CN109721419A (en) | A kind of preparation and method of administration of the dedicated selenium-rich nutritive fertilizer of radix tetrastigme | |
| CN107047080A (en) | A kind of grafting and breeding method of gold leaf Chinese catalpa | |
| Yoshida et al. | Relation between the behaviour of nitrogen and the nicotine synthesis in tobacco plant | |
| CN106831248A (en) | A kind of nursery soil of maple | |
| CN106305058A (en) | Transplanting method of cherry seedlings | |
| CN105296391A (en) | Preparation for accelerating stripping of pepper seeds from fruits and use method thereof | |
| CN111264223A (en) | Method for improving rooting rate of plants difficult to root | |
| CN104761357A (en) | Raspberry drop irrigation fertilizer | |
| CN110117205A (en) | A kind of rich germanium containing nutrient solution and its preparation method and application improving rapeseed Ge content |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |