CN109392935B - Smearing preparation for promoting wound healing of trunk of poplar and willow and preparation method and application thereof - Google Patents
Smearing preparation for promoting wound healing of trunk of poplar and willow and preparation method and application thereof Download PDFInfo
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- CN109392935B CN109392935B CN201811553290.7A CN201811553290A CN109392935B CN 109392935 B CN109392935 B CN 109392935B CN 201811553290 A CN201811553290 A CN 201811553290A CN 109392935 B CN109392935 B CN 109392935B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
- A01N47/30—Derivatives containing the group >N—CO—N aryl or >N—CS—N—aryl
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/08—Amines; Quaternary ammonium compounds containing oxygen or sulfur
- A01N33/10—Amines; Quaternary ammonium compounds containing oxygen or sulfur having at least one oxygen or sulfur atom directly attached to an aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N39/00—Biocides, pest repellants or attractants, or plant growth regulators containing aryloxy- or arylthio-aliphatic or cycloaliphatic compounds, containing the group or, e.g. phenoxyethylamine, phenylthio-acetonitrile, phenoxyacetone
- A01N39/02—Aryloxy-carboxylic acids; Derivatives thereof
- A01N39/04—Aryloxy-acetic acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
Abstract
The invention relates to a smearing preparation for promoting wound healing of trunk parts of poplar and willow, which comprises the following raw materials in 1000 parts by weight: 0.002-5 parts of plant growth regulator, 11-125 parts of high-molecular film forming agent, 0.1-10 parts of tackifier, 1-50 parts of inert carrier, 0.1-30 parts of antibacterial substance, 0.1-1 part of coloring indicator and the balance of water; the high-molecular film forming agent comprises 1-20 parts of polyvinyl alcohol, 5-50 parts of sodium carboxymethylcellulose, 5-50 parts of chitosan and 0-5 parts of polyethylene glycol; the medicine is nontoxic and harmless to cambium cells of the poplar and can accelerate the wound healing speed of trunk parts of the poplar; the application of the prepared smearing preparation in the healing treatment of the wounds of the trunk parts of the poplar and willow has the advantages of broad spectrum sterilization, low toxicity, no residue, safety, reliability, wound protection and the like.
Description
Technical Field
The invention relates to the technical field of tree wound treatment medicaments, in particular to a smearing preparation for promoting wound healing of trunk parts of poplar and willow as well as a preparation method and application thereof.
Background
The poplar and willow have the advantages of rich varieties, wide ecological adaptability, strong stress resistance and vitality, fast growth speed, easy propagation, high tree body, beautiful tree shape, large shading area, good effect and the like, are widely used as backbone tree species for urban greening, farmland shelter forest construction, four-side greening, road and river bank greening, and have the planting number of more than 50 hundred million female plants. Flying-cotton pollution generated by female salix populi is always a focus and hot problem of social attention for many years, and the treatment of the flying-cotton of salix populi also becomes an important measure for building ecological civilization and enhancing the welfare of the people.
At present, the main method for treating the flying catkin of the poplar and willow is to inject a flower inhibiting number I on a trunk, which inevitably forms a wound on trunk parts of the poplar and willow, creates extremely favorable conditions for invasion of rot and canker of the poplar and willow, further causes canker of the trunk parts and canker of barks, and leads to death of the trees when the trunk parts and the barks are serious. Thus, the wounds resulting from trunk injections need to be dealt with in a timely manner.
At present, most of the high-molecular film-forming agents contained in commercially available wound dressings are polyacrylamide, acrylic ester, waterborne polyurethane, modified epoxy resin and the like, and the film-forming agents have certain toxicity on cambium cells of the poplar and willow, so that the direct contact with the dressings and the death of adjacent cambium cells are caused, the generation of calluses on the bark cambium of the poplar and willow is delayed, the wounds on trunk parts of the poplar and willow cannot heal rapidly, and the healthy growth of trees is influenced.
In addition, the smearing preparation in the prior art only depends on chemical agents to heal the wound, and nutrients required by wound healing are not considered in combination with the growth of plant tissues, so that the wound healing speed is slower.
Disclosure of Invention
Aiming at the defects in the prior art, the first purpose of the invention is to provide a smearing preparation for promoting the wound healing of the trunk of the poplar and willow, which is nontoxic and harmless to cambium cells of the poplar and willow and can accelerate the wound healing of the trunk of the poplar and willow.
The first purpose of the invention is realized by the following technical scheme:
a smearing preparation for promoting wound healing of trunk parts of poplar and willow comprises the following raw materials in 1000 parts by weight: 0.002-5 parts of plant growth regulator, 11-125 parts of macromolecular film-forming agent, 0.1-10 parts of tackifier, 1-50 parts of inert carrier, 0.1-30 parts of antibacterial substance, 0.1-1 part of coloring indicator and the balance of water; the macromolecular film-forming agent comprises 1 to 20 parts of polyvinyl alcohol, 5 to 50 parts of sodium carboxymethyl cellulose, 5 to 50 parts of chitosan and 0 to 5 parts of polyethylene glycol.
By adopting the technical scheme, Carboxymethyl Cellulose or sodium Carboxymethyl Cellulose (CMC) in the high-molecular film forming agent is an anion, straight chain and water-soluble Cellulose, has no smell, smell and toxicity, and the aqueous solution of the CMC has the functions of thickening, film forming, bonding, water retention, emulsification, suspension and the like; polyethylene glycol (PEG) has good hygroscopicity, lubricity and cohesiveness. No toxicity and no irritation; chitosan (CA) is a nitrogenous polysaccharide substance, has a plurality of unique biological activities, is non-toxic, good in biocompatibility and easy to degrade, and is a biological polymer obtained by hydrolyzing and deacetylating renewable natural polymer material chitin under an alkaline condition; polyvinyl alcohol (PVA) is white powdery water-soluble polymer resin obtained by hydrolyzing polyvinyl acetate, is non-toxic and harmless, has low price, good film-forming property and high strength of a generated adhesive film; the components are substances which are beneficial or harmless to the trunk part, and a layer of film is formed after the components are applied to a cut saw, a girdling cut, a worm wound and a frozen wound of the trunk part, so that the trunk part wound is protected for a long time, the nutrient loss of the wound part is prevented, the germ infection is prevented, and the wound healing can be accelerated. The plant growth regulator can activate plant cells and further promote wound healing.
Preferably, the plant growth regulator includes cytokinins and auxins.
By adopting the technical scheme, cytokinin promotes cytokinesis and promotes differentiation and growth of various tissues; has synergistic effect with auxin, and is a plant hormone for regulating the growth and development of plant cells. The plant growth regulator prepared from cytokinin and auxin can make the cytokinin and the auxin cooperate with a high-molecular film-forming agent to play a synergistic role and promote the healing of cadre wounds. The two components coexist and can be mixed in any proportion to realize the effect of the smearing preparation of the invention.
Preferably, the cytokinin comprises 0-1 part of diphenyl urea or/and 0-1 part of p-iodophenoxyacetic acid.
Preferably, the auxin comprises 0-4 parts of naphthylacetic acid and/or 0-4 parts of 2, 4-dichlorophenoxyacetic acid.
By adopting the technical scheme, the cytokinin diphenyl urea, the p-iodophenoxyacetic acid, the auxin naphthylacetic acid and the 2, 4-dichlorophenoxyacetic acid are screened out by tissue culture, can better promote the poplar and the willow bark to generate callus and can promote the callus to grow.
Preferably, the inert carrier is diatomaceous earth.
Preferably, the antibacterial substance comprises 10.001-0.05 part of polyquaternium, 1-20 parts of potassium sorbate, 1-30 parts of titanium dioxide or 0.005-0.1 part of propyl p-hydroxybenzoate.
Preferably, the color indicator is an edible pigment.
By adopting the technical scheme, the diatomite, the calcium carbonate, the bentonite, the polyquaternium 1, the potassium sorbate, the titanium dioxide, the propyl p-hydroxybenzoate and the edible pigment are substances which are screened out by tissue culture and are harmless to the growth of the calluses of the bark cambium of the poplar and cannot cause direct contact and death of adjacent cambium cells, so that the calluses generated by the bark cambium of the poplar and the willow are accelerated, and the wounds of the trunk of the poplar and the willow can heal quickly.
Preferably, the tackifier is 0.1-10 parts by mass of 1-15% dopamine hydrochloride solution.
By adopting the technical scheme, the dopamine hydrochloride solution is a substance which is screened out by tissue culture and is harmless to the growth of the poplar bark cambium callus, and the dopamine hydrochloride can modify a high-molecular film-forming agent to enhance the film-forming strength.
The second purpose of the invention is to provide a preparation method of the smearing preparation for promoting the wound healing of the trunk part of the poplar and willow, which is realized by the following technical scheme:
a preparation method of a smearing preparation for promoting wound healing of poplar and willow trunk parts comprises the following preparation steps:
(1) adding sodium carboxymethylcellulose and/or polyethylene glycol into 200-800 mL of glacial acetic acid solution with the volume concentration of 0.1% -5%, stirring and dissolving to prepare a mixed solution A;
(2) adding chitosan into the mixed solution A, stirring for dissolving, adjusting the pH value of the solution to 5.5-6.0, and stirring for 0.5-1.0 hour to prepare a blending polymer B;
(3) adding polyvinyl alcohol into deionized water at 25 ℃ to obtain swollen polyvinyl alcohol, adding the swollen polyvinyl alcohol into the polymer blend B, adding the tackifier, the antibacterial substance, the coloring indicator and the inert carrier under stirring, and stirring for 1-2 hours to obtain a mixed solution C;
(4) adding a plant growth regulator into the mixed solution C, adjusting the pH to 6.0-7.0, and stirring for 0.5-3 hours to obtain the smearing preparation.
The third purpose of the invention is to provide the application of the smearing preparation for promoting the wound healing of the poplar and willow trunk part in the wound healing of the poplar and willow trunk part.
In conclusion, the invention has the following beneficial effects:
(1) the macromolecular film forming agent is coated on a cut saw, a girth cut, a worm wound and a frozen wound to form a layer of film, so that the wound is protected for a long time, and the film is resistant to wind, sunshine, rain and snow scouring and not easy to fall off, thereby preventing nutrient loss of the wound part and preventing germ infection; the plant growth regulator can activate plant cells and promote the wound to heal quickly;
(2) by the cooperation of the cytokinin which is nontoxic and harmless to the growth of the plants, the auxin and the high-molecular film-forming agent, the plant wound can be quickly healed under the protection of the high-molecular film-forming agent, and the wound healing speed of the plants is accelerated;
(3) the invention has the advantages of broad sterilization spectrum, low toxicity, no residue, safety, reliability, wound protection, economy, practicability and the like.
Detailed Description
The plant production regulator of the invention is obtained by screening the following method:
0.5 mg/L6-BA and various growth factors with different concentrations (shown in table 1) are added into an MS solid culture medium, after autoclaving at 121 ℃, poplar barks which are respectively sterilized by 75% ethanol and 5% sodium hypochlorite and washed by sterile water are inoculated and are placed in an artificial climate box (25 +/-0.5 ℃, 70 +/-5% RH, 6000lx (illumination intensity), 14L:10D (critical photoperiod)) for culture, and the effect results of various growth factors on the growth condition of the cambium-containing bark callus of the poplar are specifically shown in table 1.
TABLE 1 callus growth of poplar bark under different types and doses of auxin
As shown in Table 1, it can be seen that two auxins, NAA (naphthylacetic acid) and 2,4-D (2, 4-dichlorophenoxyacetic acid), have positive promoting effects on the growth of the callus of the bark of poplar, under the action of the two auxins, the callus of the bark cambium has better average differentiation rate and average growth amount in 20 days and 40 days, and the two auxins, NAA (naphthylacetic acid) and 2,4-D (2, 4-dichlorophenoxyacetic acid) are selected according to the growth rate of the callus of the cambium.
2.0 mg/L2, 4-D (2, 4-dichlorophenoxyacetic acid) and various cytokinins (shown in table 1) with different concentrations are added into an MS solid culture medium, the mixture is autoclaved at 121 ℃, poplar barks which are respectively sterilized by 75% ethanol and 5% sodium hypochlorite and washed by sterile water are inoculated and are placed in a climatic chamber (25 +/-0.5 ℃, 70 +/-5% RH, 6000lx and 14L:10D) for culture, and the effect results of various cytokinins on the growth condition of the bark callus containing cambium of the poplar are specifically shown in table 2.
As shown in Table 2, the two cytokinins of bisphenylurea and p-iodophenoxyacetic acid positively promote the growth of the callus of the bark of poplar, and the callus of bark cambium layer has better average differentiation rate and average growth amount in 20 days and 40 days, so that the two cytokinins of bisphenylurea and p-iodophenoxyacetic acid are selected.
TABLE 2 callus growth of poplar bark under different types and doses of cytokinins
The film-forming agent, the tackifier, the inert carrier and the antibacterial substance in the coating agent are substances which are screened by tissue culture and are harmless to the growth of the calluses of the bark cambium of the poplar. The method comprises adding the above substances into MS culture medium containing 0.5mg/L diphenyl urea and 2.0mg/L NAA, respectively, and aseptically culturing cambium-containing layer of poplar in artificial climate box (25 + -0.5 deg.C, 70 + -5% RH, 6000lx, 14L:10D), with specific influence effects shown in Table 3-Table 6.
As can be seen from tables 3-6, the film-forming agent, the tackifier, the inert carrier and the antibacterial substance in the coating agent are harmless to the growth of the cambium calluses of the poplar bark, and the cambium-containing barks of the poplar can generate the calluses faster than CK or the callus generation speed is not obviously different from that of CK when the poplar bark is subjected to aseptic culture.
TABLE 3 influence of the matrix on the differentiation and growth of poplar bark callus
Note: a. b, c and d represent significance of difference, the difference between the same letters is not significant, and the difference between different letters is significant.
TABLE 4 influence of Adhesives on differentiation and growth of poplar bark callus
TABLE 5 Effect of film-forming Agents on Poplar bark callus differentiation and growth
TABLE 6 Effect of other substances on the differentiation and growth of poplar bark callus
Example 1
Slowly adding 25 g of sodium carboxymethylcellulose (CMC) and 2.5 g of polyethylene glycol (PEG) into 700ml of 1% glacial acetic acid solution, and rapidly stirring and dissolving to prepare a CMC-PEG solution; then adding 25 g of Chitosan (CA), continuously stirring to dissolve and swell the CA, adjusting the pH value to 5.5-6.0, fully stirring to blend and modify the CA for 0.5 hour, and preparing a CMC-PEG-CA blend polymer; adding 6 g of polyvinyl alcohol into 250 ml of deionized water at the normal temperature of 25 ℃ to obtain swelled polyvinyl alcohol; adding the swelled polyvinyl alcohol into the CMC-PEG-CA blended polymer, adding 10 g of dopamine hydrochloride solution with the mass fraction of 4%, 4 g of potassium sorbate, 5g of titanium dioxide, 0.005g of polyquaternium 1, 0.5 g of edible pigment and 20 g of diatomite into the CMC-PEG-CA blended polymer while stirring quickly, and stirring the mixture fully for 1 hour to obtain a high-viscosity and uniform mixed solution; then 300 mg of diphenyl urea, 200 mg of p-iodophenoxyacetic acid, 300 mg of naphthylacetic acid and 200 mg of 2, 4-dichlorophenoxyacetic acid are added, 1000 g of deionized water is used for supplementing, stirring is carried out for 2.5 hours, and the pH is adjusted to 6.0, so that the smearing preparation for promoting the wound healing of the trunk part of the poplar and willow is prepared.
Example 2
Slowly adding 25 g of sodium carboxymethylcellulose into 700ml of 1% glacial acetic acid solution, and rapidly stirring to dissolve; then adding 25 g of CA, continuously stirring to dissolve and swell the CA, adjusting the pH value to 5.5-6.0, fully stirring to blend and modify the CA for 0.5 hour, and preparing a CMC-CA blend polymer; adding 6 g of polyvinyl alcohol into 250 ml of deionized water at the normal temperature of 25 ℃ to obtain swelled polyvinyl alcohol; then adding swelling polyvinyl alcohol into CMC-CA blended polymer, adding 10 g of dopamine hydrochloride solution with the mass fraction of 4%, 4 g of potassium sorbate, 0.1 g of titanium dioxide, 0.01g of propyl p-hydroxybenzoate, 0.5 g of edible pigment and 20 g of bentonite while stirring quickly, fully stirring for 1 hour to obtain a high-viscosity and uniform mixed solution, then adding 300 mg of diphenyl urea, 200 mg of p-iodophenoxyacetic acid, 400 mg of naphthylacetic acid and 200 mg of 2, 4-dichlorophenoxyacetic acid, supplementing 1000 g of deionized water, stirring to obtain a suspension, and adjusting the pH to 6.0 to prepare the smearing preparation for promoting wound healing of trunk parts of the poplar.
Example 3
Slowly adding 5g of sodium carboxymethylcellulose (CMC) into 200ml of 0.1 percent glacial acetic acid solution, and quickly stirring for dissolving; then adding 5g of Chitosan (CA), continuously stirring to dissolve and swell the CA, adjusting the pH value to 5.5-6.0, fully stirring to blend and modify the CA for 0.5 hour, and preparing a CMC-CA blended polymer; adding 1g of polyvinyl alcohol into 100 ml of deionized water at the normal temperature of 25 ℃ to obtain swelled polyvinyl alcohol; adding the swelled polyvinyl alcohol into the CMC-CA blended polymer, adding 10 g of 1% dopamine hydrochloride solution, 1g of potassium sorbate, 1g of titanium dioxide, 0.002g of polyquaternium 1, 0.01g of propyl p-hydroxybenzoate, 0.1 g of edible pigment and 1g of calcium carbonate in a rapid stirring manner, and fully stirring for 1 hour to obtain a high-viscosity and uniform mixed solution; then 300 mg of diphenyl urea and 200 mg of 2, 4-dichlorophenoxyacetic acid are added, and 1000 g of deionized water is used for supplementing, stirring is carried out for 0.5 hour, and the pH is adjusted to 7.0, thus preparing the smearing preparation for promoting the wound healing of the poplar and willow trunk.
Example 4
Slowly adding 5g of polyethylene glycol (PEG) into 800ml of 5% glacial acetic acid solution, and rapidly stirring for dissolving; then adding 20 g of Chitosan (CA), continuously stirring to dissolve and swell the CA, adjusting the pH value to 5.5-6.0, fully stirring to blend and modify the CA for 1.0 hour, and preparing a CMC-PEG-CA blend polymer; adding 10 g of polyvinyl alcohol into 500 ml of deionized water at the normal temperature of 25 ℃ to obtain swelled polyvinyl alcohol; adding the swelled polyvinyl alcohol into the CMC-PEG-CA blended polymer, adding 5g of dopamine hydrochloride solution with the mass fraction of 8%, 0.1 g of potassium sorbate, 0.5 g of edible pigment and 35 g of diatomite into the CMC-PEG-CA blended polymer while stirring quickly, and stirring the mixture fully for 1.5 hours to obtain a high-viscosity and uniform mixed solution; then 1000 mg of diphenyl urea and 4000 mg of 2, 4-dichlorophenoxyacetic acid are added, 1000 g of deionized water is used for supplementing, stirring is carried out for 2.5 hours, and the pH is adjusted to 7.0, thus preparing the smearing preparation for promoting the wound healing of the poplar and willow trunk.
Example 5
Slowly adding 50 g of sodium carboxymethylcellulose (CMC) and 5g of polyethylene glycol (PEG) into 700ml of 1% glacial acetic acid solution, and rapidly stirring and dissolving to prepare a CMC-PEG solution; then adding 50 g of Chitosan (CA), continuously stirring to dissolve and swell the CA, adjusting the pH value to 5.5-6.0, fully stirring to blend and modify the CA for 1.0 hour, and preparing a CMC-PEG-CA blend polymer; adding 20 g of polyvinyl alcohol into 500 ml of deionized water at the normal temperature of 25 ℃ to obtain swelled polyvinyl alcohol; adding the swelled polyvinyl alcohol into the CMC-PEG-CA blended polymer, adding 1g of 15 mass percent dopamine hydrochloride solution, 30 g of titanium dioxide, 1g of edible pigment and 50 g of diatomite into the CMC-PEG-CA blended polymer while stirring quickly, and stirring the mixture fully for 2 hours to obtain a high-viscosity and uniform mixed solution; then 1000 mg of p-iodophenoxyacetic acid and 4000 mg of naphthylacetic acid are added, 1000 g of deionized water is used for supplementing, stirring is carried out for 3 hours, and the pH value is adjusted to 7.0, thus preparing the smearing preparation for promoting the wound healing of the trunk part of the poplar and willow.
Example 6
Slowly adding 30 g of sodium carboxymethylcellulose (CMC) and 3 g of polyethylene glycol (PEG) into 600ml of 1% glacial acetic acid solution, and rapidly stirring and dissolving to prepare a CMC-PEG solution; then adding 25 g of Chitosan (CA), continuously stirring to dissolve and swell the CA, adjusting the pH value to 5.5-6.0, fully stirring to blend and modify the CA for 1.0 hour, and preparing a CMC-PEG-CA blend polymer; adding 10 g of polyvinyl alcohol into 300ml of deionized water at the normal temperature of 25 ℃ to obtain swelled polyvinyl alcohol; adding the swelled polyvinyl alcohol into the CMC-PEG-CA blended polymer, adding 0.1 g of dopamine hydrochloride solution with the mass fraction of 4%, 4 g of potassium sorbate, 0.001 g of polyquaternium 1, 0.005g of propyl hydroxybenzoate, 0.5 g of edible pigment and 35 g of diatomite into the CMC-PEG-CA blended polymer while stirring the mixture quickly, and stirring the mixture fully for 2 hours to obtain a high-viscosity and uniform mixed solution; then adding 1 mg of p-iodophenoxyacetic acid and 1 mg of naphthylacetic acid, supplementing 1000 g with deionized water, stirring for 3 hours, and adjusting the pH value to 7.0 to prepare the smearing preparation for promoting the wound healing of the trunk part of the poplar and willow.
Example 7
Slowly adding 35 g of sodium carboxymethylcellulose (CMC) and 3 g of polyethylene glycol (PEG) into 700ml of 1% glacial acetic acid solution, and rapidly stirring and dissolving to prepare a CMC-PEG solution; then adding 35 g of Chitosan (CA), continuously stirring to dissolve and swell the CA, adjusting the pH value to 5.5-6.0, fully stirring to blend and modify the CA for 1.0 hour, and preparing a CMC-PEG-CA blend polymer; adding 15 g of polyvinyl alcohol into 350 ml of deionized water at the normal temperature of 25 ℃ to obtain swelled polyvinyl alcohol; adding the swelled polyvinyl alcohol into the CMC-PEG-CA blended polymer, adding 10 g of dopamine hydrochloride solution with the mass fraction of 4%, 5g of titanium dioxide, 0.5 g of edible pigment and 40 g of diatomite into the CMC-PEG-CA blended polymer while stirring quickly, and fully stirring the mixture for 2 hours to obtain a high-viscosity and uniform mixed solution; then adding 250 mg of diphenyl urea, 250 mg of p-iodophenoxyacetic acid, 1500 mg of naphthylacetic acid and 1500 mg of 2, 4-dichlorophenoxyacetic acid, supplementing 1000 g of deionized water, stirring for 3 hours, and adjusting the pH value to 7.0 to prepare the smearing preparation for promoting the wound healing of the trunk parts of the poplar and willow.
Example 8
Slowly adding 5g of sodium carboxymethylcellulose (CMC) and 4 g of polyethylene glycol (PEG) into 700ml of 3% glacial acetic acid solution, and rapidly stirring and dissolving to prepare a CMC-PEG solution; then adding 5g of Chitosan (CA), continuously stirring to dissolve and swell the CA, adjusting the pH value to 5.5-6.0, fully stirring to blend and modify the CA for 1.0 hour, and preparing a CMC-PEG-CA blend polymer; adding 1g of polyvinyl alcohol into 450 ml of deionized water at the normal temperature of 25 ℃ to obtain swelled polyvinyl alcohol; adding the swelled polyvinyl alcohol into the CMC-PEG-CA blended polymer, adding 10 g of dopamine hydrochloride solution with the mass fraction of 3%, 0.05 g of polyquaternium 1, 0.5 g of edible pigment and 35 g of diatomite into the CMC-PEG-CA blended polymer while stirring quickly, and stirring the mixture fully for 2 hours to obtain a high-viscosity and uniform mixed solution; then 600 mg of diphenyl urea, 400 mg of p-iodophenoxyacetic acid, 2000 mg of naphthylacetic acid and 2000 mg of 2, 4-dichlorophenoxyacetic acid are added, 1000 g of deionized water is used for supplementing, stirring is carried out for 3 hours, and the pH is adjusted to 7.0, so that the smearing preparation for promoting the wound healing of the trunk parts of the poplar and willow is prepared.
Example 9
Slowly adding 20 g of CMC and 5g of PEG into 700ml of 1% glacial acetic acid solution, and quickly stirring to dissolve the CMC and the PEG to prepare CMC-PEG solution; then adding 30 g of CA, continuously stirring to dissolve and swell the CA, adjusting the pH value to be within the range of 5.5-6.0, fully stirring to blend and modify the CA for 0.5 hour, and preparing a CMC-PEG-CA blend polymer; then adding 8 g of polyvinyl alcohol fully swelled in 250 ml of deionized water at normal temperature and 25 ℃ into the CMC-PEG-CA blended polymer, adding 5g of dopamine hydrochloride solution with the mass fraction of 6%, 0.005g of propyl p-hydroxybenzoate, 0.5 g of edible pigment and 10 g of diatomite during the rapid stirring process, and fully stirring for 1 hour to obtain a high-viscosity and uniform solution; then adding 400 mg of diphenyl urea, 100 mg of p-iodophenoxyacetic acid, 1000 mg of naphthylacetic acid and 300 mg of 2, 4-dichlorophenoxyacetic acid, supplementing 1000 g of deionized water, stirring to obtain a suspension, and adjusting the pH value to 6.0 to prepare the smearing preparation for promoting the wound healing of the poplar and willow.
Comparative example 1
The spreading agent of comparative example 1 differs from example 1 in that:
the plant growth regulator of comparative example 1 was p-iodophenoxyacetic acid containing only 200 mg of cytokinin, no auxin, and the remainder was the same as that of example 1, and the coating agent of comparative example 1 was prepared.
Comparative example 2
The spreading agent of comparative example 2 differs from example 1 in that:
in comparative example 2, only 200 mg of auxin, 2, 4-dichlorophenoxyacetic acid and no cytokinin were used, and the remainder was the same as in example 1, and the coating agent of comparative example 2 was prepared.
Comparative example 3
The spreading agent of comparative example 3 differs from example 1 in that:
in comparative example 3, no auxin and no cytokinin were present, and the remainder was the same as in example 1, and the coating agent of comparative example 3 was prepared.
Comparative example 4
The spreading agent of comparative example 4 differs from example 1 in that:
in comparative example 4, the coating agent of comparative example 4 was prepared by adding chitosan, stirring to dissolve, adjusting the pH of the solution to 4.5, and the remainder was the same as in example 1.
Comparative example 5
The spreading agent of comparative example 5 differs from example 1 in that:
in comparative example 5, the coating agent of comparative example 5 was prepared by adding chitosan, stirring to dissolve, adjusting the pH of the solution to 8.5, and the remainder was the same as in example 1.
Comparative example 6
The coating agent of comparative example 6 was a commercially available Sichuan photo-paste coating agent.
Comparative example 7
The paint of comparative example 7 was 1.8% Xinjunan acetate salt of agricultural chemical Co., Ltd, Ci-an, which is commercially available.
Comparative example 8
The paint of comparative example 8 was a commercially available pleasant 1.60% benziothiazolinone.
Comparative example 9
Comparative example 9 is commercially available methylthionaphthaleneacetic acid.
Application example 1
The application site is the Chinese white poplar in Beijing city northern river park, and in order to treat the flying-floc problem of the Chinese white poplar, the trunk part of the Chinese white poplar is treated by adopting an injection mode, so that an injection wound is left on the trunk. The smearing preparation of the invention in the examples 1-9 and the comparative examples 1-9 is smeared on the injection wound of the populus tomentosa, so that the smearing preparation is guaranteed to be smeared on the phloem tissue of the trunk. According to 300ml/m2After the application once, the healing condition of the injection wound and the presence or absence of the ulceration condition of the disease infection were observed, and the results are shown in Table 7.
As can be seen from Table 7, the smearing preparation provided by the invention has a good treatment effect when applied to poplar wounds, is long in efficacy maintaining period, can ensure good healing of tree wounds, and has no disease infection fester. Comparing the example 1 with the example 2, the CMC, PEG and CA in the invention are synergistically blended and matched with polyvinyl alcohol, so that the optimal film forming effect can be achieved, and the wound is protected from being invaded by germs. It is understood from examples 1 to 9 that the cytokinin and auxin of the present invention exert a synergistic effect to accelerate the healing of the wound on the trunk and that the effect is the best when two components of cytokinin and two components of auxin are synergistically present. It is understood from example 1 and comparative examples 1 to 3 that the coexistence of cytokinin and auxin in the present invention is more effective in healing wounds of poplar trunk and maintaining the drug effect, and that the absence of cytokinin and auxin in the present invention (comparative example 3) results in a slower healing rate and a less effective healing of wounds of plants. If the pH value of the solution is adjusted to be beyond the range of 5.5-6.0 after the chitosan is added, stirred and dissolved in the preparation process, the smearing preparation is easy to agglomerate, the property of the smearing preparation is influenced, and the healing of the poplar wound is adversely affected (comparative example 4 and comparative example 5). Comparative example 3 was substantially identical in effect to the two healing agents commercially available (comparative examples 6-9), thus demonstrating that the spreading agent of the present invention works better than the healing agents commercially available.
TABLE 7 treatment effect of different paints on Populus tomentosa injection wounds
Application example 2
The application place is the steamed bread willow in the Beijing City, the northern river park, in order to treat the flying cotton problem of the steamed bread willow, the trunk part of the steamed bread willow adopts an injection mode to treat, so that an injection wound is left on the trunk. The smearing preparation prepared in the examples 1-9 and the comparative examples 1-9 is smeared on the injection wound of the steamed bread willow. According to 300ml/m2After the application once, the healing condition of the injection wound and the presence or absence of the festering condition of the disease bacteria infection are observed, and the results are shown in Table 8.
TABLE 8 treatment effect of different smearing agents on steamed bread willow injection wounds
As can be seen from Table 8, the liniment of the present invention has a good healing effect on the wounds of the trunk of willow, and the healing effect and effect are substantially equal to those of the poplar.
The above-mentioned embodiments are merely illustrative and not restrictive, and those skilled in the art can modify the embodiments without inventive contribution as required after reading this specification, but only fall within the scope of the claims of the present invention.
Claims (6)
1. The smearing preparation for promoting wound healing of the trunk parts of the poplar and willow is characterized by comprising the following raw materials in 1000 parts by weight: 0.002-5 parts of plant growth regulator, 11-125 parts of high-molecular film forming agent, 0.1-10 parts of tackifier, 1-50 parts of inert carrier, 0.1-30 parts of antibacterial substance, 0.1-1 part of coloring indicator and the balance of water; the high-molecular film forming agent comprises 1-20 parts of polyvinyl alcohol, 5-50 parts of sodium carboxymethylcellulose, 5-50 parts of chitosan and 0-5 parts of polyethylene glycol;
the plant growth regulator comprises cytokinin and auxin;
the cytokinin is diphenyl urea;
the auxin is 2, 4-dichlorophenoxyacetic acid;
the weight ratio of the 2, 4-dichlorophenoxyacetic acid to the diphenyl urea is 4: 1;
the preparation method of the smearing preparation for promoting the wound healing of the trunk part of the poplar and willow comprises the following preparation steps:
(1) adding sodium carboxymethylcellulose and/or polyethylene glycol into 200-800 mL of glacial acetic acid solution with volume concentration of 0.1% -5%, stirring for dissolving, and preparing to obtain a mixed solution A;
(2) adding chitosan into the mixed solution A, stirring for dissolving, adjusting the pH value of the solution to 5.5-6.0, and stirring for 0.5-1.0 hour to prepare a blending polymer B;
(3) adding polyvinyl alcohol into deionized water at 25 ℃ to obtain swollen polyvinyl alcohol, adding the swollen polyvinyl alcohol into the polymer blend B, adding the tackifier, the antibacterial substance, the coloring indicator and the inert carrier under stirring, and stirring for 1-2 hours to obtain a mixed solution C;
(4) adding a plant growth regulator into the mixed solution C, adjusting the pH to 6.0-7.0, and stirring for 0.5-3 hours to obtain the smearing preparation.
2. The liniment for promoting the healing of poplar and willow trunk wounds according to claim 1, wherein the smearing comprises: the inert carrier is diatomite.
3. The liniment for promoting the healing of poplar and willow trunk wounds according to claim 1, wherein the smearing comprises: the antibacterial substance comprises 10.001-0.05 part of polyquaternium, 0.1-20 parts of potassium sorbate, 0.1-30 parts of titanium dioxide or 0.005-0.1 part of propyl p-hydroxybenzoate.
4. The liniment for promoting the healing of poplar and willow trunk wounds according to claim 1, wherein the smearing comprises: the coloring indicator is an edible pigment.
5. The liniment for promoting the healing of poplar and willow trunk wounds according to claim 1, wherein the smearing comprises: the tackifier is 0.1-10 parts of dopamine hydrochloride solution with the mass fraction of 1-15%.
6. Use of the spread of any one of claims 1-5 for promoting wound healing in poplar trunk.
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| CN112244028A (en) * | 2020-09-30 | 2021-01-22 | 常熟理工学院 | High-adhesiveness pesticide microcapsule and preparation method thereof |
| CN113598185A (en) * | 2021-06-22 | 2021-11-05 | 河南神润生物科技有限公司 | Plant wound coating agent and preparation method thereof |
| CN114794095B (en) * | 2022-05-19 | 2023-12-22 | 青岛农业大学 | Protective agent for pruning saw cuts of fruit tree branches and application thereof |
| CN116210695A (en) * | 2023-02-27 | 2023-06-06 | 西北农林科技大学 | Compound for promoting plant wound healing and application thereof |
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