Summary of the invention
In order to overcome above deficiency of the prior art, the invention provides a kind of camellia tree grafting rescue method of the withered plant of branch on the ground, it is little that this method is implemented difficulty, can give treatment to effectively the camellia tree that acrial part in the gardens is withered.
In order to solve above-mentioned problems of the prior art, the following scheme of the invention process: a kind of camellia tree is the grafting rescue method of the withered plant of branch on the ground, it is characterized in that, in the described fringe bar grafting process, after guaranteeing to form edge alignment such as layer and phloem, squeeze the phloem junction that the juice of squeezing out drips stock and fringe bar with fresh camellia leaf.
Further, described camellia tree is the grafting rescue method of the withered plant of branch on the ground, it is characterized in that may further comprise the steps:
1) selects because damage by disease and insect or such environmental effects cause the withered dead camellia tree of terrestrial stem, withered stem is removed with the cut-out of saw cutter, remain with the underground root of physiologically active, remove the back and carry out disinfection to otch sprinkling plant wide-spectrum bactericide, and bandage with clean aseptic polyethylene adhesive tape; Simultaneously its root is regularly executed the water fertilizer treatment, handle 1-2 about week;
2) carry out otch in incision and handle, otch is the dark V-arrangement along the tree root axis, and notch shape stratification and phloem position are smeared the plant hormone of configuration;
3) select the famous and precious tea tree strain branch suitable with root otch thickness as grafting fringe bar, branch morphology upper end can keep 2-5 small stems, in the saw cutter truncation of these small stems base portions, spraying the plant wide-spectrum bactericide to otch carries out disinfection, smear the growth hormone of configuration then in incision, and bandage with clean aseptic polyethylene adhesive tape;
4) cut into the shape that is complementary with underground root otch in branch fringe bar morphology lower end, spraying the plant wide-spectrum bactericide in cut surface carries out disinfection, smear the plant hormone of configuration in branch fringe bar shaped stratification and phloem position then, to promote faster, the healing better of underground root and branch grafting fringe bar;
5) the branch fringe bar that will smear plant hormone inserts in the otch of underground root, guarantee to form edge alignment such as layer and phloem, seamless as far as possible, squeeze the phloem junction that the juice of squeezing out drips stock and fringe bar with fresh camellia leaf then, smear sealed envelope with the paraffin liquid after the sterilization treatment at last;
6) tie up with the clean aseptic polyethylene film of waterproof, when tying up earlier stock, fringe bar phloem junction V-arrangement topmost above 2cm tie up 2 and enclose, be lashed to V-arrangement following 2cm bottom then downwards always, prevent into water;
7) again whole strain plant is sprayed with the plant wide-spectrum bactericide, apply fertilizer at root simultaneously, after whole strain grafting is finished, on grafting plant, set up the sunshade net, grafting part is carried out lucifuge handle;
8) after a week was finished in whole strain grafting, the polyethylene film that fringe bar small stems incision is tied up took off, and regularly dripped 2 growth hormone and sprayed the plant wide-spectrum bactericide of a little at cutting part simultaneously;
9) make regular check on the grafting situation, to plastic film is taken off, check the germination of grafting survival situation and fringe bar after 2 months, the stock branch that does not survive or the fringe bar that does not germinate are mended connect, simultaneously camellia is carried out the moisturizing supplementary fertilizer.
Further, the liquid fertilizer prescription described in the step 1) described in step 1), step 7) and the step 9), step 7) and the step 9) is two kinds of nutrient solution and alum rich water, and the cycle of applying is 1-2 week.
Further, described nutrient solution prescription is: urea 10g, ammonium dihydrogen phosphate (ADP) 0.1g, EDTA ferrisodium salt 0.02g, potassium dihydrogen phosphate 3g, the moisture 0.2g of sodium dihydrogen phosphate, potassium nitrate 1.5g, nitrate of lime 1g, magnesium sulfate 0.5g, ironic citrate 0.01g, zinc sulphate 0.01g, iron sulfate 0.03g, copper sulphate 0.01g, manganese sulphate 0.03g, the moisture 0.017g of cobalt sulphate, boric acid powder 0.02g are dissolved in water to 10L, be 100 times of mother liquors, dilute during use to apply and get final product.
Further, described alum rich water prescription is: vegetalitas dish leaf 0.5kg, argol 2kg, soya-bean cake 2kg, beans shell 2kg, melon and fruit peel 0.5kg, rice washing water 94kg and account for the ferrous sulfate of rich water weight 1%.
Further, the preparation method of described alum rich water is: in vegetalitas dish leaf 0.5kg, argol 2kg, soya-bean cake 2kg, beans shell 2kg, melon and fruit peel 0.5kg, rice washing water 94kg input cylinder, place the fermentation 40 days of being exposed to the sun in the sun, form rich water through putrid fermentation, adding the ferrous sulfate that accounts for rich water gross weight 1% mixes, get its supernatant during use, convert and to use after water dilutes 8 times.
Further, the plant wide-spectrum bactericide is systemic fungicide described in step 1), step 3), step 4), step 7) and the step 8), is specially carbendazim, dislikes mould spirit or make every effort to overcome well, and concentration is 0.5g/L, wherein, the sprinkling cycle in the step 8) is 10 days/time.
Further, step 2) and the plant hormone described in the step 4) be that concentration is the cytokinin-like substance of 6-10mg/L and the mixed liquor of auxin substance.
Further, described cytokinin-like substance is zeatin, also has any one or more than one the combination among ribosylzeatin, dihydro zeatin, isopentenyl gland purine, kinetin, 6-benzyladenine, oxinane benzyladenine or diphenylurea or the KT-30; Described auxin substance is 6-benzyl aminopurine, heteroauxin, indolepopionic acid, indolebutyric acid, methyl, Nafusaku, naphthoxyacetic acid, naphthalene acetamide, 2,4-D, 2,4, any one or more than one combination in 5-T, 4-iodobenzene fluoroacetic acid or 2, the 4-D butyl ester.
Further, the auxin concentration described in step 3) and the step 8) is 6-10mg/L, and wherein, the cycle of smearing in the step 8) is 10 days/time, carries out simultaneously with sprinkling plant wide-spectrum bactericide.
Further, the described paraffin liquid of step 5) is that weight ratio 80% paraffin and 20% gum rosin make and form 80 ℃~100 ℃ fusing; Grafting fringe bar described in the step 3) is got the branch of hating day high, boy's face, cinnabar purple robe, high quality Shaoxing wine, big purple robe or avenging famous and precious strain such as clear and bright.
Camellia tree of the present invention is the grafting rescue method of the withered plant of branch on the ground, and with respect to prior art, beneficial effect is:
1, the grafting rescue method of the withered plant of camellia tree ground branch disclosed by the invention, on the basis of original underground root, get other camellia tree branches as the fringe bar, by grafting method the camellia tree that is about to death is survived again, come back to life; Because can only need 1-2 according to the suitable fringe bar of thickness selection thickness of subterraneous root, grafting plant just can be grown to script plant thickness, plants new camellia tree relatively, the time shortens greatly.
2, the present invention implements morely in implementation process to sterilization operation, and it is less to be contaminated by bacterial the possibility that causes cultivating failure in the grafting process, has improved the cultivation success rate greatly.
3, the whole plant of the present invention after to former camellia and grafting applies dissimilar fertilizer simultaneously, can improve the nutrient of camellia greatly, improves the success rate of camellia grafting.
4, the present invention carries out dark V-notch to stock, smear the low concentration basic element of cell division and growth hormone at formation layer and phloem simultaneously, and squeeze the juice squeeze out with the camellia leaf and spread upon the phloem junction, can significantly promote the agglutination of stock and fringe bar, obviously shorten cultivation period, got final product in general 1.5-2 month.
5, the present invention carries out paraffin and two kinds of method sealings of plastic film to grafting part, reduce the moisture loss of plant, drip cytokinin-like substance and the auxin substance of low concentration simultaneously at the flat nick place of fringe bar morphology upper end, improved the success rate of grafting.
In sum, adopt the described camellia tree of this method grafting rescue method of the withered plant of branch on the ground, success rate can reach more than 96%, has guaranteed the dual indexes of cultivation cycle and success rate.
Embodiment
Below in conjunction with embodiment the present invention is further elaborated.
Embodiment 1
A kind of camellia tree is the grafting rescue method of the withered plant of branch on the ground, it is characterized in that may further comprise the steps:
1) selects because damage by disease and insect or such environmental effects cause the withered dead camellia tree of terrestrial stem, withered stem is removed with the cut-out of saw cutter, remain with the underground root of physiologically active, remove the back and carry out disinfection to otch sprinkling plant wide-spectrum bactericide, and bandage with clean aseptic polyethylene adhesive tape; Simultaneously its root is regularly executed the water fertilizer treatment, handle 1-2 about week;
2) carry out otch in incision and handle, otch is the dark V-arrangement along the tree root axis, and notch shape stratification and phloem position are smeared the plant hormone of configuration;
3) select the branch of the hatred sky high strain suitable with root otch thickness as grafting fringe bar, branch morphology upper end can keep 2-5 small stems, in the saw cutter truncation of these small stems base portions, spraying the plant wide-spectrum bactericide to otch carries out disinfection, smear the growth hormone of configuration then in incision, and bandage with clean aseptic polyethylene adhesive tape;
4) cut into the shape that is complementary with underground root otch in branch fringe bar morphology lower end, spraying the plant wide-spectrum bactericide in cut surface carries out disinfection, smear the plant hormone of configuration in branch fringe bar shaped stratification and phloem position then, to promote faster, the healing better of underground root and branch grafting fringe bar;
5) the branch fringe bar that will smear plant hormone inserts in the otch of underground root, guarantee to form edge alignment such as layer and phloem, seamless as far as possible, squeeze the phloem junction that the juice of squeezing out drips stock and fringe bar with fresh camellia leaf then, smear sealed envelope with the paraffin liquid after the sterilization treatment at last; Described paraffin liquid is that 80% paraffin and 20% gum rosin make 80 ℃ of fusings.
6) tie up with the clean aseptic polyethylene film of waterproof, when tying up earlier stock, fringe bar phloem junction V-arrangement topmost above 2cm tie up 2 and enclose, be lashed to V-arrangement following 2cm bottom then downwards always, prevent into water;
7) again whole strain plant is sprayed with the plant wide-spectrum bactericide, apply fertilizer at root simultaneously, after whole strain grafting is finished, on grafting plant, set up the sunshade net, grafting part is carried out lucifuge handle;
8) after a week was finished in whole strain grafting, the polyethylene film that fringe bar small stems incision is tied up took off, and dripped 2 growth hormone and sprayed the plant wide-spectrum bactericide of a little at cutting part every 10 days;
9) make regular check on the grafting situation, to plastic film is taken off, graft survival rate is 93.2% after 2 months, and fringe bar germination rate is 92.5%, the stock branch that does not survive is mended connect, and simultaneously camellia is carried out the moisturizing supplementary fertilizer, can take out the tip then 2 times.Prove that through test of many times survival rate is up to more than 94.5%.
Wherein, step 1), fertilizer described in step 7) and the step 9) is two kinds of nutrient solution and alum rich water, the two is used alternatingly, the cycle that applies of fertilizer was 1 week, wherein nutrient solution prescription is: urea 10g, ammonium dihydrogen phosphate (ADP) 0.1g, EDTA ferrisodium salt 0.02g, potassium dihydrogen phosphate 3g, the moisture 0.2g of sodium dihydrogen phosphate, potassium nitrate 1.5g, nitrate of lime 1g, magnesium sulfate 0.5g, ironic citrate 0.01g, zinc sulphate 0.01g, iron sulfate 0.03g, copper sulphate 0.01g, manganese sulphate 0.03g, the moisture 0.017g of cobalt sulphate, boric acid powder 0.02g is dissolved in water to 10L, be 100 times of mother liquors, dilute during use to apply and get final product, described alum rich water prescription is: vegetalitas dish leaf 0.5kg, argol 2kg, soya-bean cake 2kg, beans shell 2kg, melon and fruit peel 0.5kg, rice washing water 94kg, with the ferrous sulfate that accounts for rich water weight 1%, the preparation method of described alum rich water is: with vegetalitas dish leaf 0.5kg, argol 2kg, soya-bean cake 2kg, beans shell 2kg, melon and fruit peel 0.5kg, in the rice washing water 94kg input cylinder, place the fermentation 40 days of being exposed to the sun in the sun, form rich water through putrid fermentation, adding the ferrous sulfate that accounts for rich water gross weight 1% mixes, get its supernatant during use, convert and to use after water dilutes 8 times.Step 2) and the plant hormone described in the step 4) be zeatin 6mg/L and heteroauxin 6mg/L; Growth hormone described in step 3) and the step 8) is that concentration is 6mg/L.
Embodiment 2
A kind of camellia tree is the grafting rescue method of the withered plant of branch on the ground, it is characterized in that may further comprise the steps:
1) selects because damage by disease and insect or such environmental effects cause the withered dead camellia tree of terrestrial stem, withered stem is removed with the cut-out of saw cutter, remain with the underground root of physiologically active, remove the back and carry out disinfection to otch sprinkling plant wide-spectrum bactericide, and bandage with clean aseptic polyethylene adhesive tape; Simultaneously its root is regularly executed the water fertilizer treatment, handle 1-2 about week;
2) carry out otch in incision and handle, otch is the dark V-arrangement along the tree root axis, and notch shape stratification and phloem position are smeared the plant hormone of configuration;
3) select the boy seasoned millet mush tree strain branch suitable with root otch thickness as grafting fringe bar, branch morphology upper end can keep 2-5 small stems, in the saw cutter truncation of these small stems base portions, spraying the plant wide-spectrum bactericide to otch carries out disinfection, smear the growth hormone of configuration then in incision, and bandage with clean aseptic polyethylene adhesive tape;
4) cut into the shape that is complementary with underground root otch in branch fringe bar morphology lower end, spraying the plant wide-spectrum bactericide in cut surface carries out disinfection, smear the plant hormone of configuration in branch fringe bar shaped stratification and phloem position then, to promote faster, the healing better of underground root and branch grafting fringe bar;
5) the branch fringe bar that will smear plant hormone inserts in the otch of underground root, guarantee to form edge alignment such as layer and phloem, seamless as far as possible, squeeze the phloem junction that the juice of squeezing out drips stock and fringe bar with fresh camellia leaf then, smear sealed envelope with the paraffin liquid after the sterilization treatment at last; Described paraffin liquid is that 80% paraffin and 20% gum rosin make 100 ℃ of fusings.
6) tie up with the clean aseptic polyethylene film of waterproof, when tying up earlier stock, fringe bar phloem junction V-arrangement topmost above 2cm tie up 2 and enclose, be lashed to V-arrangement following 2cm bottom then downwards always, prevent into water;
7) again whole strain plant is sprayed with the plant wide-spectrum bactericide, apply fertilizer at root simultaneously, after whole strain grafting is finished, on grafting plant, set up the sunshade net, grafting part is carried out lucifuge handle;
8) after whole strain grafting was finished, the polyethylene film that fringe bar small stems incision is tied up took off, and dripped 2 growth hormone and sprayed the plant wide-spectrum bactericide of a little at cutting part every 10 days;
9) make regular check on the grafting situation, to plastic film is taken off, graft survival rate is 96.7% after 1.5 months, fringe bar germination rate is 95.3%, the stock branch that does not survive is mended connect, and simultaneously camellia is carried out the moisturizing supplementary fertilizer, can take out the tip then 3 times, survival rate is up to more than 96%.
Wherein, step 1), fertilizer described in step 7) and the step 9) is two kinds of nutrient solution and alum rich water, the two is used alternatingly, the cycle that applies of fertilizer was 1 week, wherein nutrient solution prescription is: urea 10g, ammonium dihydrogen phosphate (ADP) 0.1g, EDTA ferrisodium salt 0.02g, potassium dihydrogen phosphate 3g, the moisture 0.2g of sodium dihydrogen phosphate, potassium nitrate 1.5g, nitrate of lime 1g, magnesium sulfate 0.5g, ironic citrate 0.01g, zinc sulphate 0.01g, iron sulfate 0.03g, copper sulphate 0.01g, manganese sulphate 0.03g, the moisture 0.017g of cobalt sulphate, boric acid powder 0.02g is dissolved in water to 10L, be 100 times of mother liquors, dilute during use to apply and get final product, described alum rich water prescription is: vegetalitas dish leaf 0.5kg, argol 2kg, soya-bean cake 2kg, beans shell 2kg, melon and fruit peel 0.5kg, rice washing water 94kg, with the ferrous sulfate that accounts for rich water weight 1%, the preparation method of described alum rich water is: with vegetalitas dish leaf 0.5kg, argol 2kg, soya-bean cake 2kg, beans shell 2kg, melon and fruit peel 0.5kg, in the rice washing water 94kg input cylinder, place the fermentation 40 days of being exposed to the sun in the sun, form rich water through putrid fermentation, adding the ferrous sulfate that accounts for rich water gross weight 1% mixes, get its supernatant during use, convert and to use after water dilutes 8 times.Step 2) and the plant hormone described in the step 4) be ribosylzeatin 3mg/L, kinetin 3mg/L and methyl 6mg/L; Growth hormone described in step 3) and the step 8) is that concentration is 6mg/L.
Embodiment 3
A kind of camellia tree is the grafting rescue method of the withered plant of branch on the ground, it is characterized in that may further comprise the steps:
1) selects because damage by disease and insect or such environmental effects cause the withered dead camellia tree of terrestrial stem, withered stem is removed with the cut-out of saw cutter, remain with the underground root of physiologically active, remove the back and carry out disinfection to otch sprinkling plant wide-spectrum bactericide, and bandage with clean aseptic polyethylene adhesive tape; Simultaneously its root is regularly executed the water fertilizer treatment, handle 1-2 about week;
2) carry out otch in incision and handle, otch is the dark V-arrangement of tree root bar axis, and notch shape stratification and phloem position are smeared the plant hormone of configuration;
3) select the purple robe tea tree of the cinnabar suitable with root otch thickness strain branch as grafting fringe bar, branch morphology upper end can keep 2-5 small stems, in the saw cutter truncation of these small stems base portions, spraying the plant wide-spectrum bactericide to otch carries out disinfection, smear the growth hormone of configuration then in incision, and bandage with clean aseptic polyethylene adhesive tape;
4) cut into the shape that is complementary with underground root otch in branch fringe bar morphology lower end, spraying the plant wide-spectrum bactericide in cut surface carries out disinfection, smear the plant hormone of configuration in branch fringe bar shaped stratification and phloem position then, to promote faster, the healing better of underground root and branch grafting fringe bar;
5) the branch fringe bar that will smear plant hormone inserts in the otch of underground root, guarantee to form edge alignment such as layer and phloem, seamless as far as possible, squeeze the phloem junction that the juice of squeezing out drips stock and fringe bar with fresh camellia leaf then, smear sealed envelope with the paraffin liquid after the sterilization treatment at last; Described paraffin liquid is that 80% paraffin and 20% gum rosin make 90 ℃ of fusings.
6) tie up with the clean aseptic polyethylene film of waterproof, when tying up earlier stock, fringe bar phloem junction V-arrangement topmost above 2cm tie up 2 and enclose, be lashed to V-arrangement following 2cm bottom then downwards always, prevent into water;
7) again whole strain plant is sprayed with the plant wide-spectrum bactericide, apply fertilizer at root simultaneously, after whole strain grafting is finished, on grafting plant, set up the sunshade net, grafting part is carried out lucifuge handle;
8) after whole strain grafting was finished, the polyethylene film that fringe bar small stems incision is tied up took off, and dripped 2 growth hormone and sprayed the plant wide-spectrum bactericide of a little at cutting part every 10 days;
9) make regular check on the grafting situation, to plastic film is taken off, graft survival rate is 95.8% after 2 months, fringe bar germination rate is 94.7%, the stock branch that does not survive is mended connect, and simultaneously camellia is carried out the moisturizing supplementary fertilizer, can take out the tip then 2 times, survival rate is up to more than 96.2%.
Wherein, step 1), fertilizer described in step 7) and the step 9) is two kinds of nutrient solution and alum rich water, the two is used alternatingly, the cycle that applies of fertilizer was 1 week, wherein nutrient solution prescription is: urea 10g, ammonium dihydrogen phosphate (ADP) 0.1g, EDTA ferrisodium salt 0.02g, potassium dihydrogen phosphate 3g, the moisture 0.2g of sodium dihydrogen phosphate, potassium nitrate 1.5g, nitrate of lime 1g, magnesium sulfate 0.5g, ironic citrate 0.01g, zinc sulphate 0.01g, iron sulfate 0.03g, copper sulphate 0.01g, manganese sulphate 0.03g, the moisture 0.017g of cobalt sulphate, boric acid powder 0.02g is dissolved in water to 10L, be 100 times of mother liquors, dilute during use to apply and get final product, described alum rich water prescription is: vegetalitas dish leaf 0.5kg, argol 2kg, soya-bean cake 2kg, beans shell 2kg, melon and fruit peel 0.5kg, rice washing water 94kg, with the ferrous sulfate that accounts for rich water weight 1%, the preparation method of described alum rich water is: with vegetalitas dish leaf 0.5kg, argol 2kg, soya-bean cake 2kg, beans shell 2kg, melon and fruit peel 0.5kg, in the rice washing water 94kg input cylinder, place the fermentation 40 days of being exposed to the sun in the sun, form rich water through putrid fermentation, adding the ferrous sulfate that accounts for rich water gross weight 1% mixes, get its supernatant during use, convert and to use after water dilutes 8 times.Step 2) and the plant hormone described in the step 4) be 6-benzyladenine 6mg/L, 2,4-D is 3mg/L and heteroauxin 3mg/L; Growth hormone described in step 3) and the step 8) is that concentration is 6mg/L.