CN104541857B - Method for cuttage seedling breeding in Myrica rubra Dongkui nutrition bowl - Google Patents
Method for cuttage seedling breeding in Myrica rubra Dongkui nutrition bowl Download PDFInfo
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- CN104541857B CN104541857B CN201410788684.6A CN201410788684A CN104541857B CN 104541857 B CN104541857 B CN 104541857B CN 201410788684 A CN201410788684 A CN 201410788684A CN 104541857 B CN104541857 B CN 104541857B
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- cuttage
- cutting
- seedling breeding
- heteroauxing
- myrica rubra
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention relates to a method for cuttage seedling breeding in a Myrica rubra Dongkui nutrition bowl. A cuttage strip base part is soaked in silver nitrate solution for half an hour; the cuttage strip base part is treated by indolacetic acid and the like; a mixture of peat and perlite serves as a substrate; cuttage seedling breeding is performed in a nutrition bowl. According to the seedling breeding method, the seedling breeding period is short (the whole seedling breeding period lasts for 9 months); the seedling breeding quality is high; the transplantation survival rate can be up to 100 percent and is obviously higher than that of seedbed seedling breeding; the method is convenient to operate and high in efficiency.
Description
(1) technical field
The present invention relates to a kind of Myrica rubra Dongkui nutritive cube cuttage and seedling culture method.
(2) background technology
Fructus Myricae rubrae is the distinctive fruit of south China, great competitiveness in domestic and international market, Zhejiang be Fructus Myricae rubrae produce and
The big province of scientific research, Fructus Myricae rubrae cultivated area has 1,300,000 mu, 5,000,000 mu of national cultivated area, and in southwest, growth momentum is fast at present
Suddenly, need substantial amounts of health Fructus Myricae rubrae nursery stock.At present Fructus Myricae rubrae nursery stock breed main based on graft seedling growth.Propagation by grafiting advantage is
Fruit tree improved seeds characteristic can be kept, but fruit tree juvenile phase is longer, reproduction speed is slower, and manual labor is relatively costly.Cuttage is numerous
Educating on fruit tree is also important method for culturing seedlings, especially Fructus Vitis viniferae (Yang Hongshuan, 2008) upper using extensively, survival rate is 90%
More than.The processing method of the cuttage material and environmental condition influence research more (Wang little Rong etc., 2003) to adventitious root.Myrica(Myrica)
Cuttage root-taking difficulty seeds, cuttage survival rate is low, Fructus Myricae rubrae cutting propagation have a small amount of report (king comes greatly, 1999；Li Minghong etc.,
2008), but be all the bearing-age tree annual shoot that grows directly from seeds that make use of life in 15～30 years, also do not have for current main breed Myrica rubra Dongkui
There is any report.
Eastern chief is the kind that in current Fructus Myricae rubrae industry, cultivated area is the widest, commodity property is best, but Myrica rubra Dongkui growth potential
By force, especially in first operation period often because growth potential is strong, go into operation late, early yield is low, of poor benefits.Cutting propagation main root is undeveloped, with
The growth potential of Myrica rubra Dongkui based on fibrous root, can be weakened, therefore, in order that Myrica rubra Dongkui can do sth. in advance result, in order to shorten nursery
In the cycle, reduce labour costs it is necessary to cutting propagation Improvement to Myrica rubra Dongkui, be desirably to obtain efficient method for culturing seedlings,
The healthy nursery stock of culture, shortening the juvenile phase, early operation, early income.
(3) content of the invention
The object of the invention is to provide a kind of Myrica rubra Dongkui nutritive cube cuttage efficient, that nursery stage is short, Quality of Seedlings is high to educate
Seedling-growing method.
The technical solution used in the present invention is：
A kind of Myrica rubra Dongkui nutritive cube cuttage and seedling culture method, methods described includes：
(1) cutting prepares：Select 15 years raw health Myrica rubra Dongkui tree annual shootes, take its taper 8～12cm, stay 3～4
Leaf, fringe bar upper cut truncation, lower cut are cut sth. askew, as cutting；Typically carry out in annual mid-June；
(2) cutting pretreatment：It is little that cutting base portion 2～3cm first soaks 5～6 with the silver nitrate solution of 0.1～0.2% (w/w)
When, then soak 20～30min with pretreatment fluid；Described pretreatment fluid composition is as follows：Heteroauxing 300～400mg/kg, first uses
Ethanol in proper amount dissolves heteroauxing, then is settled to desired concn with water；
(3) cuttage：Contain, in nutritive cube, the substrate being mixed by peat and perlite, insert cutting；
(4) maintenance：Nutritive cube is placed on seedlings nursing plate, is covered with plastics Small plastic shed, carefully sprays clear water with watering pot daily,
Keep in Small plastic shed relative humidity more than 80%, natural lighting, until nursery completes, transplant mid-March next year to gentle slope soil
On the ground.
Preferably, described substrate is peat：Perlite mass ratio 5:1 mixture.
Preferably, described pretreatment fluid composition is as follows：Heteroauxing 400mg/kg, first dissolves indole second with ethanol in proper amount
Acid, then it is settled to desired concn with water.
The effective of the present invention is mainly reflected in：The inventive method nursery stage short (whole nursery stage needs 9 months), nursery
Quality is high, using method for culturing seedlings of the present invention, transplanting survival rate up to 100%, be significantly higher than seedbed nursery, and easy to operate,
Efficiency high.
(4) specific embodiment
With reference to specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in
This：
Embodiment 1：
1 materials and methods
1.1 cuttings prepare
Select the annual shoot of 15 years raw health Myrica rubra Dongkui tree crown bottoms, take its taper 8～12cm, stay 3～4 leaves, fringe
Bar upper cut truncation, from lower end bud 0.5cm, lower cut is cut sth. askew using the back side, and otch will smooth and can not rupture.
1.2 experimental design
Cutting base portion 2～3cm is soaked 6 hours with 0.2% silver nitrate solution, then with heteroauxing or naphthalene acid solution
(adding or without sucrose and vitamin B1) is processed 30 minutes.Every kind of hormone solution soaks cutting 900, an average formula two
Insertion fills substrate (peat to part respectively：Perlite=5:1) in nutritive cube and in the seedbed of yellow soil.10 kinds of processing modes are shown in
Table 1.
Table 1
1.4 cottage method
Nutritive cube is fitly emitted on seedlings nursing plate, pours water in seedlings nursing plate and nursery, seeks at each after cutting treatment is good
The careful insertion of foster alms bowl one, the cutting density of yellow soil is also 5cm × 5cm.
1.5 nursery bed management methods
Covered using plastics Small plastic shed on seedlings nursing plate and nursery.Carefully sprayed with watering pot daily, keep phase in Small plastic shed
To humidity more than 80%.
1.6 nursery stock transplanting
After the Survival rate of nursery stock of nutritive cube and nursery lot, mid-March next year transplants to gentle slope acid soil on the ground, to end of the year next year
Investigation plant percent, transplanting survival rate=(transplant survival Seedling number/transplanted seedling number) × 100%.
1.7 data survey
After cuttage, 5 months investigation Survival rate of nursery stock strain numbers, calculate survival rate, cuttage survival rate=(surviving strain number/total strain number)
× 100%.Randomly choose 10 representative plant surviving from every process, measure the longest branch top length and the rugosity of new pumping,
Statistic diameters is more than the branch top number of 2cm, measures the length of root the longest and the rugosity of new pumping, and statistic diameters is more than the root of 2cm
Bar number, average.DPS software duncan's new multiple range method analyzes significant difference.
2 results and analysis
The Fructus Myricae rubrae cutting of water process does not survive, the cutting that 4 kinds of hormone solution soak, general using nutritive cube substrate seedling
All over the seedbed cuttage better than yellow soil, wherein process 3 and 4 cutting survival rate highests, be 54.4-57.8%, the two is not notable
Sex differernce.Process 1, process 2 and process 5 cutting survival rates for 30.4-45.8%.Cutting survival rate in yellow soil seedbed is all 18%
Below.
The newborn branch top length of nutrient matrix feeding block seedlings raising and rugosity are 6-9.8cm and 1.8-2.1cm.Yellow soil seedbed
It is 6.3cm except process 5 surviving Seedling new life branch top length,, all in below 5cm, rugosity is between 1.4-1.6cm for other.Two kinds are not
Slightly most differences are not notable for same seedling raising mannerses tip length and the tip.Newborn tip quantity is essentially identical.
Nutrient matrix nursery survives Miao Genchang between 8.8-9cm, yellow soil seedbed except process 5 a length of 9.2cm, its
He is 4.2-5.8cm.It is 3 that nutrient matrix nursery survives Seedling radical process 2 less, and other 3 are processed as 5-6 root, yellow soil
Process 5 radical be 4, but other process all only have 2.The rugosity of root, the root processing 8 is slightly 0.7cm, other all process
The thick 1-1.5cm of root, and difference is not notable.
Next year transplanting survival rate using feeding block seedlings raising of the present invention is all 100%, and the survival rate of seedling nursery is in 76-
85%, the two significant difference.
Table 2：The Fructus Myricae rubrae Seedling growth parameter(s) of 5 months after cuttage
Note：Lower case is 5% significant level, and letter is mutually all does not have significant difference.
3 discussion
Result of study of the present invention finds, after the annual twig of Myrica rubra Dongkui is soaked 6 hours with 0.2% silver nitrate solution, then
Vitamin B1 solution soaking half with the sucrose+1mg/L of 400mg/kg heteroauxing or 400mg/kg heteroauxing+2% is little
When, then cuttage is in peat：Perlite=5:In 1 nutritive cube, cutting survival rate is 54.4-57.8%.This is less than Fructus Vitis viniferae
Survival rate 75.85-93.33%, the tannin yield that this is likely due to Fructus Vitis viniferae is far less than Fructus Myricae rubrae, easier than Fructus Myricae rubrae takes root.
From production cost and simple to operate on can directly select 400mg/kg heteroauxing soak half an hour.
Indolebutyric acid (200mg/L) processes Fructus Myricae rubrae Seedling cutting of growing directly from seeds, and (king comes greatly cuttage root-taking survival rate 70-80%
1999), high than present invention survival rate, possible cause be silver nitrate soak the cutting time be 12 hours, than the present invention long 6 little when,
Hormone solution soak time is 2-4 hour, it is also possible to Myrica rubra Dongkui cutting characteristic and reality when 1.5-3.5 longer than the present invention is little
Caused by raw Seedling Fructus Myricae rubrae cutting property difference.Although cuttage survival rate of the present invention is lower slightly, work efficiency is higher than king and comes greatly
Research, the present invention in a few days can complete in a job, but the method come greatly of king due to long soaking time it is impossible to one
Individual work in a few days completes.And indolebutyric acid is used for Myrica rubra Dongkui, under equal conditions, cuttage survival rate is only 30% about,
It can be seen that the present invention adopt heteroauxing process after cuttage cultivating seedling survival rate apparently higher than indolebutyric acid.The present invention adopts nutritive cube to contain
The method nursery of substrate, transplanting survival rate is significantly higher than seedbed nursery, and easy to operate.
Claims (2)
1. a kind of Myrica rubra Dongkui nutritive cube cuttage and seedling culture method, methods described includes：
(1) cutting prepares：Select 15 years raw health Myrica rubra Dongkui tree annual shootes, take its taper 8～12cm, stay 3～4 leaves, fringe
Bar upper cut truncation, lower cut are cut sth. askew, as cutting；
(2) cutting pretreatment：Cutting base portion 2～3cm is first soaked 5～6 hours with 0.1～0.2% silver nitrate solution, then is located with pre-
Reason immersion steeps 20～30min；Described pretreatment fluid composition is as follows：Heteroauxing 300～400mg/kg, is first dissolved with ethanol in proper amount
Heteroauxing, then it is settled to desired concn with water；
(3) cuttage：Contain, in nutritive cube, the substrate being mixed by peat and perlite, insert cutting；Described substrate is peat：
Perlite mass ratio 5:1 mixture；
(4) maintenance：Nutritive cube is placed on seedlings nursing plate, is covered with plastics Small plastic shed, carefully sprays clear water with watering pot daily, keeps
In Small plastic shed, more than 80%, natural lighting, until nursery completes relative humidity.
2. the method for claim 1 is it is characterised in that described pretreatment fluid composition is as follows：Heteroauxing 400mg/kg,
First dissolve heteroauxing with ethanol in proper amount, then be settled to desired concn with water.
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| CN201410788684.6A CN104541857B (en) | 2014-12-17 | 2014-12-17 | Method for cuttage seedling breeding in Myrica rubra Dongkui nutrition bowl |
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| CN104541857B true CN104541857B (en) | 2017-02-22 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105230460A (en) * | 2015-10-23 | 2016-01-13 | 张正阳 | Cutting propagation method by utilizing twigs of broussonetia papyrifera |
| CN105594573B (en) * | 2016-03-02 | 2018-02-06 | 云南省农业科学院花卉研究所 | A kind of method for shortening camellia commodity potted flower juvenile phase |
| CN105766456A (en) * | 2016-03-04 | 2016-07-20 | 谭家友 | Planting soil suitable for waxberry |
| CN105794784B (en) * | 2016-04-06 | 2018-03-27 | 江西农业大学 | Extend method, the medicament at ornamental type rape florescence and preparation method thereof |
| CN111149670B (en) * | 2020-01-14 | 2021-08-10 | 浙江省柑桔研究所 | Soilless cultivation method for waxberry seedlings |
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| CN1180671C (en) * | 2002-06-07 | 2004-12-22 | 葛路美 | Nutrient solution spray cuttage |
| CN100393193C (en) * | 2002-10-18 | 2008-06-11 | 何园球 | High survival rate technology for transplanting red babyberry in large area |
| CN102742446A (en) * | 2012-07-27 | 2012-10-24 | 黄颖宏 | Method for cutting and breeding myrica rubra seedlings |
| CN103202161B (en) * | 2013-04-15 | 2018-04-27 | 上海国兴农现代农业发展股份有限公司 | The breeding method of Myrica rubra Dongkui |
| CN103931461B (en) * | 2014-04-22 | 2015-10-07 | 沙洲职业工学院 | The red bayberry implantation methods of green safety |
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