CN103535277A - Method for efficiently producing clocasia escalenta Schott microsphere stems - Google Patents
Method for efficiently producing clocasia escalenta Schott microsphere stems Download PDFInfo
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- CN103535277A CN103535277A CN201310429988.9A CN201310429988A CN103535277A CN 103535277 A CN103535277 A CN 103535277A CN 201310429988 A CN201310429988 A CN 201310429988A CN 103535277 A CN103535277 A CN 103535277A
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Abstract
The invention discloses a method for efficiently producing clocasia escalenta Schott microsphere stems. According to the method, clocasia escalenta Schott test-tube microsphere stems are produced through taking clocasia escalenta Schott virus-free seedlings, which are subjected to main virus removal by stem apex meristem culture, as materials under the condition of tissue culture, and inducing test-tube seedlings to form the microsphere stems through controlling the ingredients of a culture medium, the culture conditions and the ambient temperature. According to the method, the propagation speed is high, the production efficiency is high, the production cost is low, the annual factory production can be achieved, and the produced test-tube microsphere stems have the characteristics of no virus, small size and convenience in storage and transportation, so that the method is an advanced technology which has an industry prospect and can fundamentally solve the problem of parent clocasia escalenta degeneration.
Description
Technical field
The invention belongs to Plant Tissue Breeding and technical field of bioengineering.Relate in particular to a kind of method of High-efficient Production taro with red buds microballoon stem.
Background technology
Plant Tissue Breeding is one of most widely used technology in biotechnology, current tissue culture detoxicating and fast breeding technique successful Application on the Main Economic crops such as potato, sweet potato, sugarcane, strawberry, the induction of test tube microballoon stem is the technology of a comparative maturity on the plants such as potato, the tuber of pinellia, but the production of test tube microballoon stem has no report on taro with red buds is produced.
Taro with red buds (
clocasia escalenta Schott) be the improved seeds of taro, the terminal bud of bulb and lateral bud bract are red therefore named taro with red buds, are commonly called as " taro ".Monocot genus, Araeceae, perennial root herbaceous plant, leaf is avette, and petiole is long and loose, brightly yellowish green, underground have a meat bulb, and edible part is rich in starch, protein and fat, can make vegetables or staple food.Taro with red buds, because of its meat pine, fragrant, very easily boils, and unique flavor and having long enjoyed a good reputation is subject to consumer's favor deeply.< < Amplification on Materia Medica > > records taro and has the appetizing gulping down, and logical intestines close, and the broken blood of cooking postpartum, drinks its juice, hemostasis, thirsty; Benefit stomach, invigorating the spleen, wide intestines, defaecation; Removing toxic substances, dissipating bind, reduce phlegm, swelling and pain relieving; Bowl spares liver and kidney benefiting, regulate in the effect such as gas, sperm adding and marrow healthing.Therefore, taro with red buds economic worth is high, using it as medicine food dual purpose plant, has good development prospect.Taro with red buds vegetative propagation, tradition is planted the production of taro, the many generation breedings in field, virus accumulation, plant kind sexual involution is serious.The application of detoxicating cuvette microballoon stem is to solve the effective technology that taro with red buds kind taro degenerates, improves taro with red buds productivity effect.The present invention is that program is cultivated in a kind of simplification with footwork, has omitted the female seedling rooting incubation in strong sprout, reduces test-tube plantlet cultivation cycle, improves inoculation efficiency, saves manually, and the energy, is a kind of effective way that reduces test tube microballoon stem production cost.Two key technologies are depended in the application of test tube microballoon stem, the one, and whether the production efficiency of test tube microballoon stem meets large-scale production requirement, and the 2nd, the production cost of test tube microballoon stem can not be too high, and the former is prerequisite, solves on this basis field supporting technology problem.
summary of the invention
A kind of method that the object of this invention is to provide High-efficient Production taro with red buds microballoon stem.
The present invention is achieved through the following technical solutions:
A kind of method of High-efficient Production taro with red buds microballoon stem, it is characterized in that, it is material that the method be take the virus-free seedling of detoxification taro with red buds that tissue cultivates, the condition of culture of high-sucrose concentration, short-day and low temperature is the primary condition of induction test tube potato, adopt MS medium, under illumination, temperature match curing conditions induction, cultivation, obtain test tube microballoon stem.
Described medium is MS medium, do not add any somatotropin, sucrose concentration is 6-8%, intensity of illumination is 1500-2000Lx, every day photoperiod 8-10 hour, according to taro with red buds kind taro, form the responsive characteristic to short-day and low temperature, by illumination, alternating temperature, regulate, realize the production of the test tube microballoon stem of taro with red buds.
The induction of described taro with red buds test tube microballoon stem is cultivated to adopt and is cultivated with footwork, by thering is single seedling of 3-5 sheet leaf or the seedling of growing thickly in proliferated culture medium, be directly inoculated on microballoon stem inducing culture, impel plant base portion to form test tube microballoon stem, and 3 seedlings below leaf are seeded in the cultivation of proliferated culture medium continuation propagation, go round and begin again, the anniversary produces.Object is to accelerate the breeding of test tube microballoon stem, shortens the production cycle, enhances productivity, and reduces production costs.
Beneficial effect of the present invention:
In the present invention, taro with red buds test tube microballoon stem refers under conditions of tissue culture, and the taro with red buds detoxic seedling that main virus is sloughed in the process shoot apical meristem cultivation of take is material, and by controlling medium component and condition of culture and environmental temperature, induction forms test tube microballoon stem.The advantages such as that the test tube microballoon stem of producing has is virus-free, volume is little, storage convenient transportation, simultaneously, by same footwork breeding, can significantly reduce production costs, enhance productivity, there is the remarkable advantages such as industrial and annual production, be one and there is industrial prospect, fundamentally solve the advanced technology that kind of taro degenerates.
Accompanying drawing explanation
Fig. 1 is stem apex detoxify.
Fig. 2 is the induction of test tube microballoon stem.
Fig. 3 is the microballoon stem of taro with red buds list seedling induction.
Fig. 4 is taro with red buds test tube microballoon stem.
Embodiment
Embodiment 1:
The design of taro with red buds test tube microballoon stem medium: MS medium, add sucrose 6%(60g/L), agar powder 0.3%(3 g/L) be mixed with solid culture medium, adjusting pH is 5.8, the bottled 30ml of every cultivation, under 121 ℃ of high temperature, the sterilizing of 20min left and right is standby; Every bottle graft kind taro with red buds detoxification test tube plantlet 6-8 strain (clump), is 1500Lx in intensity of illumination, controls 8-10 hour every day photoperiod, cultivates 3 months, and average every strain (clump) is gathered in the crops 1.5, and every 100 blake bottles accumulative total is gathered in the crops test tube microballoon stem 900-1200 grain.
Embodiment 2:
The design of taro with red buds test tube microballoon stem medium: MS medium, add sucrose 8%(80 g/L), agar powder 0.3%(3 g/L) be mixed with solid culture medium, adjusting pH is 5.8, bottled 30 ml of every cultivation, under 121 ℃ of high temperature, the sterilizing of 20min left and right is standby; Every bottle graft kind taro with red buds detoxification test tube plantlet 6-8 strain/clump, is 2000Lx in intensity of illumination, controls about 8-10 hour every day photoperiod, cultivates 3 months, and average every strain (clump) is gathered in the crops 2, and every 100 blake bottles accumulative total is gathered in the crops test tube microballoon stem 1200-1600 grain.
Embodiment 3:
The design of taro with red buds test tube microballoon stem medium: MS medium, add sucrose 8%(80 g/L), agar powder 0.3%(3 g/L) be mixed with solid culture medium, the bottled 30ml of every cultivation, adjusting pH is 5.8, under 121 ℃ of high temperature, the sterilizing of 20min left and right is standby; Every bottle graft kind taro with red buds detoxification test tube plantlet 6-8 strain/clump, under the illumination that is 2000Lx in intensity of illumination, cultivate 30 days, control about 8 hours every days of photoperiod, then turning natural daylight cultivates, cultivate altogether 3 months, 2.5 of average every strain/clump results, every 100 blake bottles accumulative total is gathered in the crops test tube microballoon stem 1500-2000 grain.
Embodiment 4:
The design of taro with red buds test tube microballoon stem medium: MS medium, configuration macroelement and trace element, add sucrose 8%(80 g/L), agar powder 0.3%(3 g/L) be mixed with solid culture medium, the bottled 30ml of every cultivation, adjusting pH is 5.8, under 121 ℃ of high temperature, 23min sterilizing is standby; Every bottle graft kind taro with red buds detoxification test tube plantlet 6-8 strain, Fluctuation temperature culture (25 ± 3 ℃ of daytimes, night is at 18-20 ℃), under the illumination that is 2000Lx in intensity of illumination, cultivate 30 days, control about 8 hours every days of photoperiod, then turn natural daylight and cultivate, cultivate altogether 3 months, 3 of average every strain/clump results, every 100 blake bottles accumulative total is gathered in the crops test tube microballoon 1800-2400 grain.
Embodiment 5:
Taro with red buds test tube microballoon stem designs with footwork: in the induction of test tube microballoon stem, from propagation, choose the test-tube plantlet individual plant seedling of 3-5 sheet leaf blake bottle or the seedling of growing thickly is transferred in inducing culture, cultivate altogether and can directly induce into microballoon stem in 3 months, the test-tube plantlet that is less than 3 leaves continues to be transferred to synchronous propagation breeding in proliferated culture medium, by that analogy, can realize by stages and in groups and producing in the ceaselessly anniversary.Every batch of output of total growth=year production batch x.
Claims (3)
1. the method for a High-efficient Production taro with red buds microballoon stem, it is characterized in that, it is material that the method be take the virus-free seedling of detoxification taro with red buds that tissue cultivates, the condition of culture of high-sucrose concentration, short-day and low temperature is the primary condition of induction test tube potato, adopt MS medium, under illumination, temperature match curing conditions induction, cultivation, obtain test tube microballoon stem.
2. the method for High-efficient Production taro with red buds microballoon stem according to claim 1, it is characterized in that, described medium is MS medium, and or not containing any somatotropin, its sucrose concentration is not 6-8%, be placed in blake bottle, intensity of illumination is 1500-2000Lx, controls 8-10 hour every day photoperiod, according to taro with red buds kind taro, forms the responsive characteristic to short-day and low temperature, by illumination, temperature, regulate, realize the test tube microballoon stem of taro with red buds and produce.
3. the method for the method of High-efficient Production taro with red buds microballoon stem according to claim 1, it is characterized in that, the production of described taro with red buds test tube microballoon stem adopts bulb induction to cultivate with test-tube plantlet propagation and cultivates with footwork, be about to there is single seedling of 3-5 sheet leaf in proliferated culture medium or the seedling of growing thickly is directly inoculated on microballoon stem inducing culture, impel plant base portion to form test tube microballoon stem, and 3 seedlings below leaf continue propagation cultivation.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145680A (en) * | 2014-08-18 | 2014-11-19 | 江西省江天农业科技有限公司 | Method for propagating detoxification colocasia konishii hayata seeds |
| CN104145682A (en) * | 2014-08-18 | 2014-11-19 | 安徽省农业科学院园艺研究所 | Method for planting detoxification colocasia konishii hayata test-tube micro corms |
| CN109329026A (en) * | 2018-11-30 | 2019-02-15 | 青岛农业大学 | A kind of method that high temperature-intermittent warming obtains Sweetpotato Viruses Elimination seedling |
| CN114788495A (en) * | 2022-04-07 | 2022-07-26 | 南充市农业科学院 | Hormone-free strawberry stem tip culture method |
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| JPH078189B1 (en) * | 1986-12-02 | 1995-02-01 | Kyowa Hakko Kogyo Kk | |
| CN1179882A (en) * | 1997-10-07 | 1998-04-29 | 南京农业大学 | Rapid bulb propagation method for betel nut taro seeds |
| CN102217550A (en) * | 2011-06-09 | 2011-10-19 | 马宗新 | Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
-
2013
- 2013-09-18 CN CN201310429988.9A patent/CN103535277A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH078189B1 (en) * | 1986-12-02 | 1995-02-01 | Kyowa Hakko Kogyo Kk | |
| CN1179882A (en) * | 1997-10-07 | 1998-04-29 | 南京农业大学 | Rapid bulb propagation method for betel nut taro seeds |
| CN102217550A (en) * | 2011-06-09 | 2011-10-19 | 马宗新 | Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
Non-Patent Citations (2)
| Title |
|---|
| YUJI YAMAMOTO等: ""IN VITRO CORM FORMATION AND GROWTH HABIT OF PROPAGATED SEED CORM IN TARO (COLOCASIA ESCULENTA SCHOTT.)"", 《JOURNAL OF THE JAPANESE SOCIETY FOR HORTICULTURAL SCIENCE》, vol. 61, no. 1, 31 December 1992 (1992-12-31), pages 55 - 61 * |
| 崔瑾: ""芋（Colocasia esculenta L. Schott）脱毒快繁体系的构建以及组培苗无糖培养的研究"", 《中国优秀博硕士学位论文全文数据库(博士) 农业科技辑》, no. 1, 15 March 2003 (2003-03-15), pages 048 - 2 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145680A (en) * | 2014-08-18 | 2014-11-19 | 江西省江天农业科技有限公司 | Method for propagating detoxification colocasia konishii hayata seeds |
| CN104145682A (en) * | 2014-08-18 | 2014-11-19 | 安徽省农业科学院园艺研究所 | Method for planting detoxification colocasia konishii hayata test-tube micro corms |
| CN109329026A (en) * | 2018-11-30 | 2019-02-15 | 青岛农业大学 | A kind of method that high temperature-intermittent warming obtains Sweetpotato Viruses Elimination seedling |
| CN114788495A (en) * | 2022-04-07 | 2022-07-26 | 南充市农业科学院 | Hormone-free strawberry stem tip culture method |
| CN114788495B (en) * | 2022-04-07 | 2023-04-14 | 南充市农业科学院 | Hormone-free strawberry stem tip culture method |
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