CN1223261C - Test tube quick-breeding method for konjak - Google Patents
Test tube quick-breeding method for konjak Download PDFInfo
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- CN1223261C CN1223261C CN 02155216 CN02155216A CN1223261C CN 1223261 C CN1223261 C CN 1223261C CN 02155216 CN02155216 CN 02155216 CN 02155216 A CN02155216 A CN 02155216A CN 1223261 C CN1223261 C CN 1223261C
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Abstract
The present invention relates to a test-tube konjak quick propagating method which comprises: basal parts of test-tube adventitious buds induced by isolated culture of corms of konjaks or bud scales or petiole tissue are directly expanded or enriched into small corms (i.e. test-tube miniature taros) without a bud forming stage under appropriate culture condition, and the small corms are directly used as propagation materials. The method can overcome the defects of in-situ transplantation of test-tube buds. Moreover, the present invention has the advantages of few operation links, low production cost, annual industrial production and no seasonal limitation. Meanwhile, the present invention can realize production, storage and transportation in different places, and is free from region limitation.
Description
Technical field: the present invention relates to agricultural biological technical field, particularly relate to a kind of method of utilizing plant tissue cultured in vitro technology artificial quick culturing Amorphophallus rivieri kind taro in blake bottle.
Background technology: 55%～60% of konjak corm dry substance mixture, glucomannans.Konjak glucomannan is a kind of good solubility hemicellulose, have health care function and unique physicochemical properties such as anticancer, fat-reducing and reducing blood lipid, have bigger using value at aspects such as food, medicine, oil gas drilling, textile printing and dyeing, chemical industry and environmental protection.Simultaneously, suitable kind of konjaku in concealment cool, the high mountain or half high mountain of height above sea level 600-2000 rice, wide geographic area in provinces and cities such as the southern Sichuan of China, Chongqing, Yunnan, Guizhou, Shan Nan, west place in Hubeis is suitable for plantation, is a kind of can allow peasant programme, development local economy and the important poverty alleviation of foreign exchange earning and high benefit economic crops of processing type.Therefore, since the eighties, konjaku obtains more deep development and use in China, formation section, farming, worker, merchant, the complete industry of trade.But, slowly cause kind of a taro scarcity owing to planting the taro breeding, limited the expansion and the new varieties of this crops planting area and promoted, become and press for solution and insoluble problem in the production.
Because the gene height mixes, konjaku sexual propagation offspring proterties is extremely unstable, so will not adopt in the seed production of sexual propagation.Though the stripping and slicing breeding can slightly improve reproduction coefficient (4～8), because otch is perishable, incidence of disease height is also seldom used at present.The breeding of konjaku continues the offspring by the root-like stock that produces on the product organ bulb in fact entirely in the production.The root-like stock germination and growth becomes 1 year little konjaku of life, and the above konjaku of life in 2 years could produce root-like stock once more.As China's main breed elephant-foot yam (Amorphophallus konjac C.Koch), the bulb of life in 2 years can produce 1～2 root-like stock, and the bulb of life in 3～4 years can produce 4～8 root-like stocks, and average reproduction coefficient is in theory about 4.But when the konjaku market were good, the little konjaku about life in a large amount of 2 years was also by baking processing, so the reproduction coefficient of konjaku reality is 2～4.So low reproduction coefficient is difficult to the satisfied wilderness demand of going up konjaku kind taro of producing.
Utilizing the method for tissue culture to carry out micropropagation of plants has been a kind of success and feasible method.Nineteen fifty-threes such as the Morrel of the U.S. are reported konjaku tissue culture regeneration plant first.The middle and later periods eighties, the many scholars of China such as Zhang Xingguo have made a large amount of konjakus and have organized cultured in vitro research for the purpose of quick culturing Amorphophallus rivieri, all obtain regeneration plant, but do not break through " soon " word in the breeding, therefore the propagating system of being set up does not have practical value.Zhang Xingguo etc. took the lead in having broken through technically the technical barrier that was expanded numerous millions of test tube indefinite buds or test-tube plantlet by a konjak corm in a year in 1992 at home and abroad, first the test tube indefinite bud was superscribed people's work post skin and succeeded in developing konjak artificial seed (Zhang Xingguo etc., 1993) again in 1993.Improve through the several years subsequently, it is ripe that these two technology (konjaku test-tube plantlet propagation technique and artificial seed propagation technique) are tending towards, quick culturing Amorphophallus rivieri on a small scale, but large-scale fast numerous remaining in technical matters.The former: (1) operation link is many, as emerging in blake bottle, carry out root induction, will and practice seedling to soil disinfection during transplanting; (2) the operating technology level requires highly, low as the root regeneration ability of konjaku, and the regeneration capacity of the unique compound leaf of overground part is lower, so very careful in the transplanting process, guarantees that the injury-free and slow as early as possible seedling of blade recovers to grow; (3) the test-tube plantlet field-transplanting has seasonality and timeliness, promptly must carry out and must carry out in season at the konjaku suitable growth in the non-strong sunlight period of fine day, and must guarantee after the plantation sufficiently long growth time is arranged; (4) enough konjaku test-tube plantlet planting-sites must be arranged near fast numerous factory, because konjaku test-tube plantlet inconvenience long-distance transport; (5) recruitment is more, reproductive-cost is higher.Although the latter becomes the seedling stage, does not practice seedling and transplanting and produce and plant and can carry out in the strange land without test tube, but necessary aseptic parcel and axenic germination, otherwise artificial seed is sprouted into the conversion ratio of seedling low (the konjak artificial seed conversion ratio of non-sterile parcel and sprouting reaches 70% once in a while, and poor repeatability).
Summary of the invention: the present invention forms on above-mentioned two technical foundation and improves, its purpose is to provide a kind of neither Cheng Miao in vitro, also unlike artificial seed, need superscribe people's work post skin, but directly the test tube indefinite bud is cultivated the miniature taro that can independently breed, have operation link, low, the practical konjaku kind taro method for quickly breeding of production cost.
Substance of the present invention is to organize cultured in vitro to induce the test tube indefinite bud of generation konjak corm, perula sheet or petiole, and its base portion being expanded enrich without becoming the seedling stage under the suitable culture condition becomes bottom set, the i.e. miniature taro of test tube.
The present invention realizes by following steps:
Get konjak corm, perula sheet or petiole tissue, after surface sterilization, be cut into the square fritter of 0.5～1cm, be inoculated on the MS medium of additional 0.5～3mg/L BA, 0.1～2mg/L KT, 0～1mg/L NAA, 0～1mg/L IAA, 2～4% sucrose and 0.7% agar, the generation of evoked callus and the differentiation of indefinite bud, cut indefinite bud, stripped aseptic cultivation on above-mentioned medium, bastem portion being expanded enrich becomes bottom set.
Advantage of the present invention and good effect are:
(1) this invention is cultivated into the miniature taro of test tube with konjaku test tube indefinite bud and directly as propagating materials, was both cultivated, practices processes such as seedling and transplanting without test-tube plantlet, does not also need to wrap up people's work post skin, has therefore reduced operation link, has reduced production cost.
(2) planting taro can produce in the anniversary batch production, is not subject to seasonal restrictions, and also can produce, preserve and transportation in the strange land simultaneously, not limited by the region.
(3) the present invention can quick culturing Amorphophallus rivieri kind taro, especially konjaku breeding in the popularization of the numerous and new varieties of the rapid expansion of fine individual plant.
Embodiment:
Embodiment 1:
Get konjak corm, after surface sterilization, be cut into the square fritter of 0.5cm, be inoculated on the MS medium of additional 0.5mg/L BA, 0.1mg/L KT, 3% sucrose and 0.7% agar, the generation of evoked callus and the differentiation of indefinite bud, cut indefinite bud, stripped aseptic cultivation on above-mentioned medium, bastem portion being expanded enrich becomes bottom set.
Embodiment 2:
Get konjaku perula sheet, after surface sterilization, be cut into the square fritter of 1cm, be inoculated on the MS medium of additional 3mg/L BA, 2mg/LKT, 1mg/L NAA, 4% sucrose and 0.7% agar, the generation of evoked callus and the differentiation of indefinite bud, cut indefinite bud, stripped aseptic cultivation on above-mentioned medium, bastem portion being expanded enrich becomes bottom set.
Embodiment 3:
Get konjaku petiole tissue, after surface sterilization, be cut into the square fritter of 0.8cm, be inoculated on the MS medium of additional 3mg/L BA, 2mg/L KT, 1mg/L IAA, 4% sucrose and 0.7% agar, the generation of evoked callus and the differentiation of indefinite bud, cut indefinite bud, stripped aseptic cultivation on above-mentioned medium, bastem portion being expanded enrich becomes bottom set.
Embodiment 4:
Get konjak corm, after surface sterilization, be cut into the square fritter of 0.7cm, be inoculated on the MS medium of additional 1.5mg/L BA, 1mg/L KT, 0.5mg/L NAA, 3% sucrose and 0.7% agar, the generation of evoked callus and the differentiation of indefinite bud, cut indefinite bud, stripped aseptic cultivation on above-mentioned medium, bastem portion being expanded enrich becomes bottom set.
Embodiment 5:
Get konjaku petiole tissue, after surface sterilization, be cut into the square fritter of 0.5cm, be inoculated on the MS medium of additional 3mg/L BA, 1mg/L KT, 0.5mg/L IAA, 3% sucrose and 0.7% agar, the generation of evoked callus and the differentiation of indefinite bud, cut indefinite bud, stripped aseptic cultivation on above-mentioned medium, bastem portion being expanded enrich becomes bottom set.
Embodiment 6:
Get konjak corm, after surface sterilization, be cut into the square fritter of 0.5cm, be inoculated on the MS medium of additional 2mg/L BA, 1mg/LKT, 0.1mg/L IAA, 2% sucrose and 0.7% agar, the generation of evoked callus and the differentiation of indefinite bud, cut indefinite bud, stripped aseptic cultivation on above-mentioned medium, bastem portion being expanded enrich becomes bottom set.
Claims (1)
1, a kind of konjaku test-tube taro method for quickly breeding, it is characterized in that realizing: get konjak corm, perula sheet or petiole tissue by following steps, after surface sterilization, be cut into the square fritter of 0.5～1cm, be inoculated on the MS medium of additional 0.5～3mg/LBA, 0.1～2mg/L KT, 0～1mg/L NAA, 0～1mg/L IAA, 2～4% sucrose and 0.7% agar, the generation of evoked callus and the differentiation of indefinite bud, cut indefinite bud, stripped aseptic cultivation on above-mentioned medium, bastem portion being expanded enrich becomes bottom set.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02155216 CN1223261C (en) | 2002-12-10 | 2002-12-10 | Test tube quick-breeding method for konjak |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 02155216 CN1223261C (en) | 2002-12-10 | 2002-12-10 | Test tube quick-breeding method for konjak |
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| CN1422526A CN1422526A (en) | 2003-06-11 |
| CN1223261C true CN1223261C (en) | 2005-10-19 |
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| CN 02155216 Expired - Fee Related CN1223261C (en) | 2002-12-10 | 2002-12-10 | Test tube quick-breeding method for konjak |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100469238C (en) * | 2006-02-20 | 2009-03-18 | 云南师范大学 | Method for producing test-tube carmus for high efficiency commercialized prodn. of devilstrongue |
| CN102763597B (en) * | 2012-08-15 | 2013-11-20 | 云南省农业科学院生物技术与种质资源研究所 | Two-generation in one year reproduction method for konjac cross breeding |
| CN103430822B (en) * | 2013-09-09 | 2015-03-18 | 云南省农业科学院生物技术与种质资源研究所 | Aquaculture seed reproduction method for micro seed tubers of konjac |
| CN104623212A (en) * | 2015-01-14 | 2015-05-20 | 孙振双 | Chinese medicinal composition for treating strain of lumbar muscles and application thereof |
| CN105432301B (en) * | 2015-12-07 | 2018-10-26 | 竹溪县佳兴魔芋食品有限公司 | A method of improving konjaku vegetative propagation rate |
| CN113973717A (en) * | 2021-11-29 | 2022-01-28 | 云南省热带作物科学研究所 | Germination accelerating and seedling raising method for bulbil konjak tissue culture micro-corms |
| CN115191348B (en) * | 2022-07-28 | 2023-06-06 | 安康市农业科学研究院 | Batch induction method of konjak mutants |
| CN116267606B (en) * | 2023-02-28 | 2024-01-09 | 大连工业大学 | Rapid propagation method of konjak seeds |
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