CN1295790A - Amorphophallus rivieri group seedling-culturing batch production and cultivation technology - Google Patents

Abstract

The present invention adopts identical culture medium to make inoculation and subculture according to the conventional operation condition, it can obtain seedling by one step in only 45 days, then one subculture is achieved at an interval of 20 days, its reproduction multiples are 1:4 or 1:6; then the rooting culture brings about rooting in 12-15 days, and the obtained young plant with culture stem of cleaned root portion is stably placed in the seminary, and under the conditions of that the temp. is 20-30 deg.C. humidity is 80-90% and sunshading degree is 50%, the seedling grows up, and at the same time, 1-6 ppm of paclobutrazol and nutrient solution are applied. Said invention can implement batch production of group-cultured seedlings, its growing period is short, cost is low, and it can obtain above 50% of seed konjak in current year.

Description

Mass production of konjaku tissue cultivating seedling and tissue cultivating seedling culture technique

The present invention relates to mass production of a kind of konjaku tissue cultivating seedling and tissue cultivating seedling culture technique, belong to agro-ecology and agriculture cultivation technology field.

Because contained dextran has extensive, special purposes in the konjaku, causes the interest and the attention of many countries.Since 80 years, constantly the someone adopts two to go on foot into the work that the seedling method is cooked the konjaku tissue culture, and to influencing the propagation of konjaku group in training kind, take root etc. various factors is studied, and a small amount of tissue cultivating seedling is moved into field production, and obtains the taro kind about the 8-30 gram.And the kind taro that is used to produce needs the campaign in 3 years.Above-mentioned test major part is confined to laboratory research, and experimentation cost is higher.Up to now, do not see and can realize mass production konjaku tissue cultivating seedling, and can tie the taro weight average then more than 50 grams, a young plant can be tied a big taro, the separately report of 2-4 little taro.

The objective of the invention is to overcome the deficiency in the present technology, and provide a kind of and can realize forming seedling through one step culture, a seedling is multiple, growth cycle is short, cost is low, and can train mass production and tissue cultivating seedling culture technique in the above konjaku group of 50 grams tying the taro weight average then.

The present invention is achieved in that earlier flushing of konjaku explant and sterilization cut growing point, is once-seedling forming with routinely temperature and illumination control in the medium that is placed on MS+6-BA6-0.1mg/ml+NAA6-0mg/ml PH5.8 after 45 days.Again same medium is put in the tissue cultivating seedling stripping and slicing and carried out successive transfer culture, every 20 days subcultures once, the propagation multiple is 1: 4-1: 6, and the controlled condition in the successive transfer culture process is same as the prior art.Culture of rootage is that the seedling that grows to 3-4 centimetre is cut, and put into MS+NAA5-lmg/ml medium 12-15 days is bottle outlet after sending out roots.Condition in the process of rooting culture is same as the prior art.Konjaku tissue cultivating seedling culture technique is to clean the medium of seedling root earlier with 0.1% carbendazim solution, subsequently seedling being moved in the seedbed of 15 centimetres of seeding row spacing 15 cm x, is that 20-30 ℃, humidity are that 80-90%, sunshade degree are to grow under 50% the condition in temperature.And changing paclobutrazol and the nutrient solution that seedling applies 1-6ppm period, the concentration ratio of nutrient solution is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1.Wherein the every first quarter moon of nutrient solution applies once, applies once at the every 4-5 of stem tuber expanding stage days, until the stem tuber maturation.

Embodiments of the invention are: light washes tuberous konjaku explant, and get the terminal bud part for specification by 0.5 centimetre of 0.5 cm x, soaked 15 minutes with alcohol-pickled 2-3 second of 75% with 0.1% mercuric chloride respectively again, use aseptic water washing immediately, konjaku stem tuber explant after the flushing is put into MS+6BA6-0.1mg/ml+NAA6-0mg/ml PH5.8 medium, in temperature is more than 20 degree, cultivates in the environment of illuminance 1000Lx and gets final product forming seedling through one step culture in 45 days.Put into tissue cultivating seedling same cultivation stem again and carry out successive transfer culture this moment, and every 20 days subcultures once, the propagation multiple is l: 4-1: 6, and the temperature in the successive transfer culture process is more than 20 ℃, illumination is 1000Lx.Cut when seedling grows to 3-4 centimetre, put into MS+NAA5-1mg/ml medium 12-15 days is bottle outlet after sending out roots, and the temperature of control and illuminance are identical with the successive transfer culture process.Before the cultivation, earlier with the cultivation stem of seedling root with 0.1% carbendazim solution cleaning, subsequently seedling is moved in the seedbed of 15 centimetres of seeding row spacing 15 cm x, be that 20-30 ℃, humidity are that 80-90%, sunshade degree are to grow under 50% the condition in temperature.And changing paclobutrazol and the nutrient solution that seedling applies 1-6ppm period, the ratio of nutrient solution is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1.Wherein the every first quarter moon of nutrient solution applies once, applies once at the every 4-5 of stem tuber expanding stage days, until the stem tuber maturation.Weight was general all more than 50 grams when stem tuber was ripe, and several taros grow 2-4 root-like stock again mostly, have enlarged reproduction coefficient, have reduced cost, and the tissue cultivating seedling that present technique is produced is the kind taro with regard to obtaining to can be used for producing in two years.

The present invention compared with prior art has inoculation and subculture process and all adopts the same culture medium, can be real Existing forming seedling through one step culture, a seedling is multiple, growth cycle is short, cost is low, and can realize the training seedling production of mass group and Obtain then the above advantages such as kind taro of 50 grams.

Claims (2)

1, a kind of konjaku tissue cultivating seedling mass production technology, form by konjaku explant, flushing, sterilization, inoculation, successive transfer culture, culture of rootage operation, it is characterized in that this production technology adopts the forming seedling through one step culture method, promptly inoculate and the training operation that continues all adopts with a kind of training basicly, the composition of medium is MS+6-BA6-0.1mg/ml+NAA6-0mglmPH5.8; In the inoculation operation, the konjaku explant after the sterilization in medium 45 days can once-seedling forming; Successive transfer culture every 20-25 days subcultures once, propagation multiple be 1: 4-1: 6; Culture of rootage is that the seedling that grows to 3-4 centimetre is cut open, and put into MS+NAA5-1mg/ml medium 12-15 days is bottle outlet after sending out roots.

2, a kind of konjaku tissue cultivating seedling culture technique, it is characterized in that cleaning with 0.1% carbendazim solution earlier the cultivation stem of seedling root, subsequently seedling being moved in the seedbed of 15 centimetres of seeding row spacing 15 cm x, is that 20-30 ℃, humidity are that 80-90%, sunshade degree are to grow under 50% the condition in temperature; And changing paclobutrazol and the nutrient solution that seedling applies 1-6ppm period, the concentration ratio of nutrient solution is N: P: K: Ca: Mg=5: 1: 5: 0.5: 1; Wherein the every first quarter moon of nutrient solution applies once, applies once at the every 4-5 of stem tuber expanding stage days, until the stem tuber maturation.