CN108184588A - A kind of efficient method for transplanting of konjaku tissue-cultured seedling - Google Patents
A kind of efficient method for transplanting of konjaku tissue-cultured seedling Download PDFInfo
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- CN108184588A CN108184588A CN201810040530.7A CN201810040530A CN108184588A CN 108184588 A CN108184588 A CN 108184588A CN 201810040530 A CN201810040530 A CN 201810040530A CN 108184588 A CN108184588 A CN 108184588A
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- tissue culture
- konjaku
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention belongs to breed of crop technical field, more particularly to a kind of efficient method for transplanting of konjaku tissue-cultured seedling is equipped with including selection and grows fine, cultivated two weeks at the light and airing that the tissue culture seedling of base portion band root bottle is placed on temperature≤30 DEG C, humidity is 60 80%；Nutrient solution is prepared, is carried out disinfection processing using the mixed liquor of streptomysin and Thiodiazole-copper；It will be fixed with haydite in tissue culture seedling replanting to field planting basket to be transplanted, maintain nurturing an environment temperature≤30 DEG C, spraying applies nutrient solution.Tissue-cultured seedling can grow new root for 34 days in method for transplanting of the present invention, and survival rate is up to more than 90%, two months bottom sets that can grow 10g or so sizes so that whole year can carry out multiple batches of konjaku kind production, greatly improve the production efficiency of konjaku.
Description
Technical field
The invention belongs to breed of crop technical field, more particularly to a kind of efficient method for transplanting of konjaku tissue-cultured seedling.
Background technology
Konjaku You is Betel Betel, terrible taro, terrible head, and ancient Chinese is also known as bewitching taro, and the title for just having " removing intestines sand " since ancient times is day
Southern Star section belongs to perennial root herbaceous plant.Konjaku is grown under sparse woods, is beneficial basic food, to edible animal
The excessive people of acid food, collocation eat konjaku, can reach food acid, soda balance.The bulb of konjaku is more rich in Glucomannan etc.
Kind of active ingredient, has a higher medical and edible value, cold in nature, acrid flavour, toxic, and major function is detumescence, attacks poison, external application
Cure mainly the diseases such as ulcerative carbuncle, pyogenic infections, caking and venomous snake bite, the tuberculosis of cervical lymph nodes, hernia etc..It is one as food after konjaku processing
Kind gel food, health-care effect protrude, and fiber content is high, can promote gastrointestinal peristalsis, and human consumption is helped to absorb, and elimination is cardiovascular
Fatty deposit on wall has good therapeutic effect to obesity, constipation, hyperlipidemia and diabetes etc..Thus konjaku is widely used
In fields such as food, medicine, chemical industry and biologies.
Konjaku mainly carries out vegetative propagation on production is promoted using taro whip and bulb, and breeding coefficient is relatively low, generally
4-6 root-like stock can only be generated, in addition with kind of demand is big and the reasons such as each strain is impure from generation to generation so that the expansion of new varieties it is numerous and
Rapid promote is limited by very large.In recent years, using group culturation rapid propagating technology so that carrying out industry using root-like stock and bulb
Change breeding to be possibly realized, substantially reduce repoductive time, largely solve that ganoid konjaku taro breeding coefficient is low, introduces a collection is insufficient asks
Topic, still, existing tissue-cultured seedling soil transplanting survival rate are very low, directly influence implementing and applying for this technology, for this purpose, this
Application explores a kind of efficient method for transplanting of konjaku tissue-cultured seedling.
Invention content
Tissue-cultured seedling can grow new root for 3-4 days in method for transplanting of the present invention, and survival rate is up to more than 90%, two months
Grow the bottom set of 10g or so sizes so that whole year can carry out multiple batches of konjaku kind production, greatly improve the life of konjaku
Produce efficiency.
The present invention provides a kind of efficient method for transplanting of konjaku tissue-cultured seedling, include the following steps：
S1：Selection is grown fine, the tissue culture seedling of base portion band root is placed on temperature≤30 DEG C, humidity is the saturating of 60-80%
Light ventilation is cultivated two weeks, obtains the tissue culture seedling after hardening；
S2：It is nutrient solution that 5.5-7.0, EC value are 250-400 μ s/cm to prepare pH, using the mixed of streptomysin and Thiodiazole-copper
It closes liquid to carry out disinfection to the tissue culture seedling base portion after hardening in S1 processing, it is young to obtain tissue culture to be transplanted by disinfecting time 15-20min
Seedling；
S3：The whole strain of tissue culture seedling to be transplanted in S2 is transplanted in field planting basket, is fixed with haydite, maintains nurturing an environment temperature
≤ 30 DEG C of degree, spraying apply the nutrient solution prepared in S2, and with the rate of 500-600ml/min, per 40-50min, spraying is primary, spray
Fog duration is 1-2min, and after 38-42 days, with the rate of 500-600ml/min, per 20-30min, spraying is primary, and spraying continues
Time is 5-10min, harvests bulb after two months, can make konjaku kind.
Preferably, the tissue culture seedling of S1 is cultivated in culture bottle, and tissue culture seedling is cultivated one week in the S1
When unscrew bottle cap, when culture two weeks, opens bottle cap completely, and then addition aqua sterilisa is to just covering media surface.
Preferably, a concentration of 3000mg/L of the streptomysin, a concentration of 4000mg/L of the Thiodiazole-copper, and the two
With volume ratio 1:1 mixing.
Preferably, in S2, every liter of nutrient solution is mixed by following components：Ca(NO3)2918mg/L, KH2PO4
136mg/L, NH4NO3329mg/L, KNO3554mg/L, MgSO4493mg/L, H3BO32.86mg/L MnSO4·4H2O
2.13mg/L ZnSO4·7H2O 0.22mg/L, CuSO4·5H2O0.08mg/L, (NH4)6Mo4O4·4H2O 0.02mg/L steam
Distilled water is settled to 1L.
Compared with prior art, beneficial effects of the present invention：
Fixation of the haydite to tissue culture seedling and the air permeable humidity retaining for root play a good role, and are trained using spraying
Support technology, new root can be grown within general 3-4 days, this for konjaku tissue-cultured seedling can transplant survival play the role of it is key；And
Survival rate is up to more than 90%, and the time for tying konjaku kind is short, and two months bottom sets that can grow 10g or so sizes, whole year can
It is produced with carrying out multiple batches of konjaku kind, greatly improves the production efficiency of konjaku.
Specific embodiment
Several specific embodiments of the present invention are described in detail below, it is to be understood that protection scope of the present invention
It is not restricted by specific implementation.
Embodiment 1
A kind of efficient method for transplanting of konjaku tissue-cultured seedling, includes the following steps：
S1：Four months bottoms, choose grow fine equipped with 600 plants altogether, the tissue culture seedling of base portion band root bottle be placed on 25 DEG C, it is wet
It spends at the light and airing for 60% and cultivates two weeks, obtain the tissue culture seedling after hardening, tissue culture seedling unscrews tissue culture children when cultivating one week
Seedling bottle cap, enables air to enter in bottle, tissue culture seedling bottle cap is opened completely when cultivating two weeks, then adds in aqua sterilisa and is overflow to firm
Cross media surface；
S2：Preparation pH is 5.5, the nutrient solution that EC values are 250 μ s/cm, and the composition of nutrient solution is：Ca(NO3)2918mg/L,
KH2PO4136mg/L, NH4NO3329mg/L, KNO3554mg/L, MgSO4493mg/L, H3BO32.86mg/L MnSO4·
4H2O 2.13mg/L, ZnSO4·7H2O 0.22mg/L, CuSO4·5H2O 0.08mg/L, (NH4)6Mo4O4·4H2O
0.02mg/L, distilled water are settled to 1L；Using the mixed liquor of agricultural streptomycin and Thiodiazole-copper to the tissue culture seedling after hardening in S1
Base portion progress soaking disinfection processing, a concentration of 3000mg/L of agricultural streptomycin, a concentration of 4000mg/L of Thiodiazole-copper, and two
Person is with volume ratio 1:1 mixing, disinfecting time 15min obtain tissue culture seedling to be transplanted；
S3：It treats that seedling trails, the whole strain of tissue culture seedling to be transplanted in S2 is transplanted in field planting basket, is fixed with haydite,
25 DEG C of nurturing an environment temperature is maintained, spraying applies the nutrient solution prepared in S2, with the spraying one per 40min of the rate of 500ml/min
Secondary, the spraying duration is 1min, and primary with the every 20min sprayings of the rate of 500ml/min after 40 days, the spraying duration is
5min harvests the konjak corm of 10g or so after two months.
To verify the advantageous effect of nutrient solution in the present invention, using contrast experiment, respectively by existing a variety of konjaku nutrition
Nutrient solution in formula of liquid alternative embodiment 1, the transplanting method of Application Example carries out konjaku tissue culture transplantation of seedlings, as a result as follows：
Influence of 1 nutrient solution prescription of table to transplanting effect
| Nutrient solution prescription | Transplanting survival rate (%) | New root generated time (my god) | Tie the potato time (my god) |
| Jiri STAJNER formula | 46 | 10-16 | 50-70 |
| Japanese garden formula formula | 38 | 15-22 | 60-80 |
| Water culture of potato nutrient solution | 76 | 6-10 | 45-60 |
| Huo Gelan is formulated | 63 | 12-18 | 55-70 |
| MS is formulated | 62 | 8-10 | 40-60 |
| Inventive formulation | 92 | 3-4 | 30-40 |
Understand that nutrient solution is transplanted in the application present invention by data in table 1, transplanting survival rate is high, rooting rate is fast,
The knot potato time is short, and nutrient solution prescription composition is substantially better than existing a variety of nutrient solution prescriptions on the market.
Embodiment 2
S1：Early May, choose grow fine equipped with 420 plants altogether, the tissue culture seedling of base portion band root bottle be placed on 28 DEG C with
Under, cultivate two weeks at the light and airing that humidity is 80%, obtain the tissue culture seedling after hardening, when tissue culture seedling culture one week unscrews group
Seedling bottle cap is trained, enables air to enter in bottle, tissue culture seedling bottle cap is opened completely when cultivating two weeks, then add in aqua sterilisa extremely
Just cover media surface；
S2：Preparation pH is 7.0, the nutrient solution that EC values are 400 μ s/cm, and the composition of nutrient solution is：Ca(NO3)2918mg/L,
KH2PO4136mg/L, NH4NO3329mg/L, KNO3554mg/L, MgSO4493mg/L, H3BO32.86mg/L MnSO4·
4H2O 2.13mg/L, ZnSO4·7H2O 0.22mg/L, CuSO4·5H2O 0.08mg/L, (NH4)6Mo4O4·4H2O
0.02mg/L, distilled water are settled to 1L；Using the mixed liquor of agricultural streptomycin and Thiodiazole-copper to the tissue culture seedling after hardening in S1
Base portion immersion carries out disinfection processing, a concentration of 3000mg/L of agricultural streptomycin, a concentration of 4000mg/L of Thiodiazole-copper, and two
Person is with volume ratio 1:1 mixing, disinfecting time 20min obtain tissue culture seedling to be transplanted；
S3：It treats that seedling trails, the whole strain of tissue culture seedling to be transplanted in S2 is transplanted in field planting basket, is fixed with haydite,
28 DEG C of nurturing an environment temperature is maintained, spraying applies the nutrient solution prepared in S2, with the spraying one per 50min of the rate of 600ml/min
Secondary, the spraying duration is 2min, and primary with the every 30min sprayings of the rate of 600ml/min after 38 days, the spraying duration is
10min harvests the konjak corm of 10g or so after two months.
Nutrient solution is applied by field planting basket spray pattern in embodiment 2, for nutrient solution application side in the verification present invention
The advantageous effect of formula, using contrast experiment, the nutrient solution of control group is applied using tissue-cultured seedling in seedbed by the way of tide irrigation,
As a result it is as follows：
Influence of the 2 nutrient solution method of application of table to transplanting effect
| Nutrient solution method of application | Transplanting survival rate (%) | Rootage duration (my god) | Harvest bulb fresh weight |
| Method of application of the present invention | 94 | 3-4 | 9-12.7 |
| Tide seedbed is irrigated | 80 | 5-8 | 3.3-7.4 |
Understand nutrient solution using mist spray mode using transplanting survival rate is high, rootage duration is short, the ball of harvest by data in table 2
Stem fresh weight is big, better than tide irrigation mode.
Embodiment 3
S1：Four months bottoms, choose grow fine equipped with 720 plants altogether, the tissue culture seedling of base portion band root bottle be placed on 23 DEG C, it is wet
It spends at the light and airing for 70% and cultivates two weeks, obtain the tissue culture seedling after hardening, tissue culture seedling unscrews tissue culture children when cultivating one week
Seedling bottle cap, enables air to enter in bottle, tissue culture seedling bottle cap is opened completely when cultivating two weeks, then adds in aqua sterilisa and is overflow to firm
Cross media surface；
S2：Preparation pH is 6.0, the nutrient solution that EC values are 350 μ s/cm, and the composition of nutrient solution is：Ca(NO3)2918mg/L,
KH2PO4136mg/L, NH4NO3329mg/L, KNO3554mg/L, MgSO4493mg/L, H3BO32.86mg/L MnSO4·
4H2O 2.13mg/L, ZnSO4·7H2O 0.22mg/L, CuSO4·5H2O 0.08mg/L, (NH4)6Mo4O4·4H2O
0.02mg/L, distilled water are settled to 1L；Using the mixed liquor of agricultural streptomycin and Thiodiazole-copper to the tissue culture seedling after hardening in S1
Base portion progress soaking disinfection processing, a concentration of 3000mg/L of agricultural streptomycin, a concentration of 4000mg/L of Thiodiazole-copper, and two
Person is with volume ratio 1:1 mixing, disinfecting time 18min obtain tissue culture seedling to be transplanted；
S3：It treats that seedling trails, the whole strain of tissue culture seedling to be transplanted in S2 is transplanted in field planting basket, is fixed with haydite,
28 DEG C of nurturing an environment temperature is maintained, spraying applies the nutrient solution prepared in S2, with the spraying one per 45min of the rate of 550ml/min
Secondary, the spraying duration is 2min, and primary with the every 25min sprayings of the rate of 550ml/min after 42 days, the spraying duration is
8min harvests 697 plants of the konjak corm of 10g or so after two months, transplants success rate 97%.
It should be noted that the step method used in claims of the present invention is same as the previously described embodiments, in order to anti-
Only repeat, description of the invention preferred embodiment, but those skilled in the art once know it is basic creative general
It reads, then additional changes and modifications may be made to these embodiments.So appended claims are intended to be construed to include preferably in fact
It applies example and falls into all change and modification of the scope of the invention.
Obviously, various changes and modifications can be made to the invention without departing from essence of the invention by those skilled in the art
God and range.In this way, if these modifications and changes of the present invention belongs to the range of the claims in the present invention and its equivalent technologies
Within, then the present invention is also intended to include these modifications and variations.
Claims (4)
1. a kind of efficient method for transplanting of konjaku tissue-cultured seedling, which is characterized in that include the following steps：
S1：The light transmission that selection is grown fine, the tissue culture seedling of base portion band root is placed on temperature≤30 DEG C, humidity is 60-80% is led to
It is cultivated two weeks at wind, obtains the tissue culture seedling after hardening；
S2：It is the nutrient solution that 5.5-7.0, EC value are 250-400 μ s/cm to prepare pH, using streptomysin and the mixed liquor of Thiodiazole-copper
Carry out disinfection to the tissue culture seedling base portion after hardening in S1 processing, and disinfecting time 15-20min obtains tissue culture seedling to be transplanted；
S3：The whole strain of tissue culture seedling to be transplanted in S2 is transplanted in field planting basket, is fixed with haydite, maintenance nurturing an environment temperature≤
30 DEG C, spraying applies the nutrient solution prepared in S2, and with the rate of 500-600ml/min, per 40-50min, spraying is primary, and spraying is held
The continuous time is 1-2min, and after 38-42 days, with the rate of 500-600ml/min, per 20-30min, spraying is primary, sprays the duration
For 5-10min, bulb is harvested after two months, can make konjaku kind.
2. the efficient method for transplanting of konjaku tissue-cultured seedling as described in claim 1, which is characterized in that the tissue culture seedling of S1 is to cultivate
It is cultivated in bottle, and when tissue culture seedling is cultivated one week in the S1 unscrews bottle cap, opens bottle cap completely when cultivating two weeks, so
Aqua sterilisa is added in afterwards to just covering media surface.
3. the efficient method for transplanting of konjaku tissue-cultured seedling as described in claim 1, which is characterized in that the streptomysin it is a concentration of
3000mg/L, a concentration of 4000mg/L of the Thiodiazole-copper, and the two is with volume ratio 1:1 mixing.
4. the efficient method for transplanting of konjaku tissue-cultured seedling as described in claim 1, which is characterized in that in S2, every liter of nutrient solution by with
Lower component mixes：Ca(NO3)2918mg/L, KH2PO4136mg/L, NH4NO3329mg/L, KNO3554mg/L, MgSO4
493mg/L, H3BO32.86mg/L MnSO4·4H2O 2.13mg/L, ZnSO4·7H2O 0.22mg/L, CuSO4·5H2O
0.08mg/L, (NH4)6Mo4O4·4H2O 0.02mg/L, distilled water are settled to 1L.
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| CN201810040530.7A CN108184588B (en) | 2018-01-16 | 2018-01-16 | Efficient transplanting method for konjac tissue culture seedlings |
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| CN201810040530.7A CN108184588B (en) | 2018-01-16 | 2018-01-16 | Efficient transplanting method for konjac tissue culture seedlings |
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| CN108184588A true CN108184588A (en) | 2018-06-22 |
| CN108184588B CN108184588B (en) | 2020-06-30 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548607A (en) * | 2018-12-14 | 2019-04-02 | 湖北美荷农业科技有限公司 | A kind of nutritive cube cultural method of konjaku bulbil |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109548607A (en) * | 2018-12-14 | 2019-04-02 | 湖北美荷农业科技有限公司 | A kind of nutritive cube cultural method of konjaku bulbil |
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