CN102440132A

CN102440132A - Method for increasing transplanting success rate of tissue culture plantlets of honeysuckle

Info

Publication number: CN102440132A
Application number: CN2011102925234A
Authority: CN
Inventors: 何全慧; 李大根; 杨再江; 赵勇彪; 祝利
Original assignee: CHONGQING XIUSHAN RED STAR TCM DEVELOPMENT Co Ltd
Current assignee: CHONGQING XIUSHAN RED STAR TCM DEVELOPMENT Co Ltd
Priority date: 2011-10-04
Filing date: 2011-10-04
Publication date: 2012-05-09

Abstract

A method for increasing transplanting success rate of tissue culture plantlets of honeysuckle enables honeysuckle plants to gradually adapt to external environment by means of seedling hardening, transplanting, recovery-phase management, growth-phase management and outplanting management, so that the honeysuckle plants correspondingly vary in physiology, form and organization to be more adaptive to the nature, the success rate of the tissue culture plantlets of honeysuckle is increased greatly, and the plants grow healthily and well.

Description

Improve the honeysuckle tissue cultivating seedling and transplant the method for success rate

Technical field

The present invention relates to a kind of Plant Tissue Breeding quick propagating technology, the particularly transplanting method of honeysuckle tissue cultivating seedling.

Background technology

Honeysuckle belongs to caprifoliaceae plant, and herb all can be used as medicine, and bud contains volatile aromatic oil, inositol, saponin(e, chlorogenic acid, flavonoids, cinnamic acid, nerol, jasminal etc., contains alkaloid in the stem; Containing lonicerin and sweet-scented osmanthus element etc. in the leaf, is one of traditional Chinese medicine material, also is the raw material of medicine-food two-purpose, has clearing heat and detoxicating; Wind-heat dissipating, the effect of hepatic cholagogic is used for the swollen furunculosis of carbuncle, larynx numbness always in traditional Chinese medical science field; Erysipelas, toxic-heat and blood stasis, anemopyretic cold, the treatment of warm morbidity; More be used for health care's tea-drinking now; The Lonicera flower tea pleasant aroma; Can make beverage; Control heatstroke in midsummer, the infection of the upper respiratory tract, anemopyretic cold and gastrointestinal disease are had good effect, to removing human free radical, delay senility, improve human immunologic function etc. and have good effect, thereby also are the health-care good product of hypertension and cardiovascular system patient cholesterol reducing; Honeysuckle integrates views and admires medicinal, and economic worth is high, and market demand is very big, thereby needs a large amount of breeding honeysuckles.At present, honeysuckle mainly breeds with conventional methods such as cuttage, sowing, grafting, press strips, not only receive season limit, and survival rate is very low, can not satisfy the needs of large-scale production; Though propagation by grafiting plant adaptability is strong, the advantage of well developed root system must gather wild resource in a large number, and operation is numerous and diverse, and survival rate is also very low.Have only through the tissue culture propagating mode and could breed the honeysuckle seedling fast in enormous quantities; But, be under the relative moisture environmental condition of aseptic, nutritious supply, suitable illumination and temperature nearly 100%, to grow, therefore through the honeysuckle seedling of tissue culture; All very big difference is arranged at aspects such as physiology, forms with the seedling of natural conditions growths; When they were transplanted to booth, the seedling percentage of water loss can be very high, is very easy to dead.

Summary of the invention

Technical problem to be solved by this invention provides a kind of method that the honeysuckle tissue cultivating seedling is transplanted success rate that improves, and makes it can better adapt to external environment, makes the institutional framework of plant more substantial, with the success of assurance group training seedling replanting.

For solving the problems of the technologies described above; The technical scheme that the present invention adopted is through refining seedling, transplanting, convalescence management, growing season management and goes out the garden management; Make the honeysuckle plant little by little adapt to external environment; Thereby make physiology, form, tissue that corresponding the variation taken place, make it more appropriate to natural environment, concrete steps are following:

1. refining seedling: there is the tissue cultivating seedling of 3-4 bar root to take in the greenhouse with long, refining seedling 2-4 days, every day, light application time was 12-14 hour, intensity of illumination is 0.8-1.2 ten thousand Lxu, temperature 16-28 ℃, open the cultivation bottle cap gradually, keep air humidity greater than 60%.

2. transfer: carry out according to following steps:

matrix configuration: according to the proportional arrangement of peat: perlite=4:1; Water content of substrate 50-60%; Promptly pinched moist feeling with hand; Agglomeratingly do not loose but do not have water droplet to ooze, the matrix of the agglomerating one-tenth piece that can't break into pieces is cast out.

gets seedling, washes seedling, sterilization: gesture wants light when getting seedling; Wash seedling and will control the quantity of washing seedling, be advisable with 3 strains at every turn,, repeat to wash 2 times, carefully clean the medium of root to reduce the mechanical damage of seedling; Putting into concentration to the seedling after cleaning is that 1000-1200 carbendazim soup doubly soaks, and should not stack during immersion too much, in order to avoid damaged blade picks up gently after 1-2 minute and waits to transplant.

⑶ transplant: use plug transplantation, transplant gesture and want light, the degree of depth buries basal part of stem and get final product in 1cm, must root be nuzzled in the soil during transplanting, transplants gently pressure matrix top layer, back, and base portion or root are fully contacted with matrix, but tension not; It is not impaired that the transplanting process will protect blade, and root system is if long can suitably the shearing keeps the long root system of 1-2cm, and transplanting depth is only dark, notes not making matrix cover blade and heart portion, in order to avoid cause rotten.

⑷ the normal root of preserving moisture: will note the work of preserving moisture in the transplanting process, will spray to the blade face after 2 hours that in order to avoid normal root water is perhaps directly drenched in blade or plant dehydration, normal root water is formed by 1000-1200 times of carbendazim soup dilution.

3. convalescence management: in the 7-15 after the transfer days is convalescence, and illumination is controlled between the 6000-8000Lux during this, and temperature is controlled between 15-28 ℃; Humidity is controlled between the 80%-95%, only carries out foliar spray in preceding 3 days, does not carry out the matrix moisturizing; The part extra dry white wine occurs like the matrix top layer and then carry out the matrix moisturizing; Treat the plant life of having stood upright, when new root is taken out and give birth to, had to young leaves, represent that this plant has become alive.

4. growing season management: from after convalescence was vegetative period within 40-55 days, and seedling will strengthen illumination after becoming alive stablizing gradually during this, and intensity of illumination is controlled in 0.6-1.8 ten thousand Lux; But cloudy day whole day illumination; Temperature is controlled between 15-30 ℃, and humidity is controlled between the 50%-80%, notes the wet situation of doing of matrix; Be affixed by 1000-1500 times execute and spend the more 30-10-10 type or 20-20-20 type rich water; Note ventilating and correct rich water quality management, prevent the generation of fungal disease, will note controlling moisture during morbidity.

5. go out garden management: about the high 10cm of plant, robust growth, no damage by disease and insect reaches the envelope row between the strain system, and root system and matrix ability are agglomerating when deviating from, and can go out the garden.

This method contacts the honeysuckle tissue cultivating seedling gradually with the external world, thereby makes its physiology, form, tissue that corresponding the variation taken place, and make it more appropriate to natural environment, improved the honeysuckle tissue cultivating seedling greatly and transplanted success rate, and plant strain growth is healthy and strong, grows fine.

Embodiment

Below in conjunction with embodiment the present invention is described further.

1. there is the tissue cultivating seedling of 3-4 bar root to take in the greenhouse 1000 strain length, refining seedling 3 days, illumination every day 14 hours, intensity of illumination is 1.2 ten thousand Lxu, temperature 16-28 ℃, keep air humidity greater than 80%, open the cultivation bottle cap gradually.

2. preparation cultivation matrix, according to the ratio preparation cultivation matrix of peat: perlite=4:1, water content of substrate 50-60%, when having pinched moist feeling with hand, agglomerating do not loose but do not have water droplet to ooze get final product.

3. take out seedling gently, each 3 strains, water is carefully cleaned the root medium; Repeat 2 times, putting into concentration then is that 1000-1200 carbendazim soup doubly soaks, and picks up gently after 2 minutes; Transplant gently in the dish of cave, transplanting depth is 1cm, gently presses the matrix top layer then; Then spray 1000-1200 carbendazim soup normal root water doubly, transplant and sprayed in the blade face in back 2 hours.

4. normal root water of spray in after this per 3 days, and illumination is controlled between the 6000-8000Lux, temperature is controlled between 15-28 ℃, humidity is controlled between the 80%-95%, about 10 days after, plant is extracted young leaves successively out, bears new root.

5. be affixed by in after this per 5 days 1000-1500 doubly execute the more 30-10-10 type rich water of flower, intensity of illumination is controlled in 0.6-1.8 ten thousand Lux, but cloudy day whole day illumination, temperature is controlled between 15-30 ℃; Humidity is controlled between the 50%-80%, and during 45 days left and right sides, plant is long successively to arrive about high 10cm; Robust growth and do not have damage by disease and insect, root system and matrix can be agglomerating be deviate from, in the time of the 55th day; It is neat all to rise, 981 strains that become to live, and survival rate reaches 98%.

The honeysuckle tissue cultivating seedling survival rate that adopts this method to transplant can reach 98%.

Claims

1. improve the method that the honeysuckle tissue cultivating seedling is transplanted success rate, it is characterized in that transplant step is refining seedling, transplanting, convalescence management, growing season management and goes out the garden management, concrete steps are following:

refines seedling: have the tissue cultivating seedling of 3-4 bar root to take in the greenhouse with long; Refining seedling 2-4 days; Every day, light application time was 12-14 hour; Intensity of illumination is 0.8-1.2 ten thousand Lux; Temperature 16-28 ℃, open the cultivation bottle cap gradually, keep air humidity greater than 60%;

transplants: take out seedling gently, and each 3-5 strain, water is carefully cleaned the root medium; Repeat 2 times; Put into concentration then and be 1000-1200 carbendazim soup doubly and soak, pick up gently after 2 minutes, transplant gently in the dish of cave in the cultivation matrix; Transplanting depth is 1cm; Gently press the matrix top layer then, then spray 1000-1200 carbendazim soup normal root water doubly, after 2 hours sprayed in the blade face;

⑶ convalescence manages: transplant normal root water of back spray in per 3 days, and illumination is controlled between the 6000-8000Lux, temperature is controlled between 15-28 ℃, and humidity is controlled between the 80%-95%;

⑷ growing season management: treat that the gold and silver flower seedling grows after young leaves bears new root; Be affixed by in per 5 days 1000-1500 doubly execute flower the more 30-10-10 type or 20-20-20 type rich water; Intensity of illumination is controlled in 0.6-1.8 ten thousand Lux; But cloudy day whole day illumination, temperature are controlled between 15-30 ℃, and humidity is controlled between the 50%-80%;

goes out the garden management: about the long high 10cm of arriving of plant; When root system and matrix can be agglomerating be deviate from, can go out the garden.

2. raising honeysuckle tissue cultivating seedling according to claim 1 is transplanted the method for success rate, it is characterized in that cultivation matrix is peat and perlitic mixture, and its mass ratio is 4:1, and water content is 50-60%.