CN102405759B - Cuttage seedling culturing method for branch culture of honeysuckle - Google Patents
Cuttage seedling culturing method for branch culture of honeysuckle Download PDFInfo
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- CN102405759B CN102405759B CN 201110292721 CN201110292721A CN102405759B CN 102405759 B CN102405759 B CN 102405759B CN 201110292721 CN201110292721 CN 201110292721 CN 201110292721 A CN201110292721 A CN 201110292721A CN 102405759 B CN102405759 B CN 102405759B
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Abstract
The invention provides a cuttage seedling culturing method for branch culture of honeysuckle. The method comprises the following steps: preparing branch culture nutrient solution; preparing a branch culture base material; cutting and picking cuttage branches; carrying out rooting culture; arranging a seedbed; carrying out cuttage; and carrying out seedling culture management. Compared with the prior art, the branch culture nutrient solution is adopted for rooting culture, and the branch culture base material is adopted, so that the survival rate of cuttage seedlings is 89 percent, and a cuttage seedling culturing method of honeysuckle with simple operation, high survival rate, strong stability and little investment is provided.
Description
Technical field
The present invention relates to the asexual reproduction method of a Plants, particularly a kind of method with cottage method breeding lonicera macranthoides honeysuckle.
Background technology
Honeysuckle belongs to caprifoliaceae plant, and herb all can be used as medicine, and bud contains volatile aromatic oil, inositol, saponin(e, chlorogenic acid, flavonoids, cinnamic acid, nerol, jasminal etc., containing alkaloid in the stem, contain lonicerin and sweet-scented osmanthus element etc. in the leaf, is one of traditional Chinese medicine material, also be the raw material of medicine-food two-purpose, have clearing heat and detoxicating, wind-heat dissipating, the effect of hepatic cholagogic is used for the swollen furunculosis of carbuncle, larynx numbness always in traditional Chinese medical science field, erysipelas, toxic-heat and blood stasis, anemopyretic cold, the treatment of warm morbidity; Now more for health care's tea-drinking, the Lonicera flower tea pleasant aroma, can make beverage, control heatstroke in midsummer, the infection of the upper respiratory tract, anemopyretic cold and gastrointestinal disease there is good effect, to removing human free radical, delay senility, improve human immunologic function etc. and have good effect, thereby it also is the health-care good product that hypertension and cardiovascular system patient reduce cholesterol; Honeysuckle integrates views and admires medicinal, and economic worth is high, and market demand is very large, thereby needs the amount reproduction honeysuckle.At present, honeysuckle mainly breeds with methods such as cuttage, sowing, grafting, press strip, group trainings, these methods respectively have quality, although such as propagation by grafiting plant strong adaptability, the advantage of well developed root system must gather wild resource in a large number, influence ecological environment, the tissue culture method operation is numerous and diverse, drops into very greatly, can't promote.
Application for a patent for invention numbers 200610050985.4 discloses " high efficiency breeding technology for lonicera macranthoides honeysuckle ", adopt advanced cottage method, but its method is numerous and diverse, and its survival rate also only has 70%.
Summary of the invention
Technical problem to be solved by this invention provide a kind of simple to operate, survival rate is high, stability is strong, the honeysuckle cuttage and seedling culture method of less investment.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is cuttage seedling culturing method for branch culture of honeysuckle, comprise preparation training branch nutrient solution, preparation training branch base-material, cut steps such as plucking cutting, culture of rootage, seedbed arrangement, cuttage, seedling management, concrete condition is as follows.
1. the branch nutrient solution is trained in preparation: with methyl α-naphthyl acetate 75mg/kg, and salicylic acid 10g/kg, B B-complex 10g/kg, metronidazole 20g/kg, sucrose 20g/kg, agar 6g/kg, activated carbon 5g/kg stirs.
2. the branch base-material is trained in preparation: make base-material with equivalent humus soil, sawdust, perlite, decomposed rock, fully mix.
3. cut and pluck cutting: clip life in 2 years, diameter 0.5-1.0cm, healthy and strong, the undamaged branch of growing way, front 3-5 days of beta pruning, tackle selected branch and carry out sterilizing with 1000 times of carbendazim dilutions, branch length 20-30cm, stay two joints, the bottom joint is from lower end 4-5cm, and top is saved from upper end 5-10cm; Tangent plane should be level and smooth, and its upper end is flat mouth, and the lower end is the inclined-plane.
4. culture of rootage: will the branch lower end insert in the training branch nutrient solution and soaked 7 days, lower joint immerses, and upper joint outside.
5. seedbed arrangement: 30-40cm is ploughed deeply in the seedbed, and hack is raked, and is made into the furrow piece of wide 1m, holds the bar ditch successfully by line-spacing 20cm, with 10000 times of carbendazim dilutions sterilizing is carried out in the seedbed, will train the branch base-material and apply in the bar ditch thickness 8-10cm, a little compacting.
6. cuttage: it is 15～30 ℃ that cutting time should be selected temperature, and the 7-9 month of humidity about 80% is if the greenhouse then can carry out at any time; Branch after will soaking during cuttage carries out oblique cutting according to line-spacing 5cm, and lower joint will be imbedded in the training branch base-material, and compacting is watered.
7. seedling management: frequent water spray, keep ground moistening, loosen the soil, weeding; Young sprout grows and pinching when three pairs of leaves occurring, inspires shoot, and again pinching when shoot grows three pairs of leaves again during height of seedling 30cm, can suitably be applied fertilizer, and every mu is spread fertilizer over the fields urea and each 3kg of potassium dihydrogen phosphate, and after growing seedlings 60 days, nursery stock can go out the garden and transplant.
The invention has the beneficial effects as follows that compared with prior art the present invention has been owing to adopted a training branch nutrient solution to carry out culture of rootage, and adopt a training branch base-material, so that the cuttage and seedling culture survival rate increases substantially.
Description of drawings
Accompanying drawing is branch culture of honeysuckle cuttage and seedling culture flow chart of the present invention.
Embodiment
The invention will be further described below in conjunction with embodiment.
1. the branch nutrient solution is trained in preparation: with methyl α-naphthyl acetate 75mg/kg, and salicylic acid 10g/kg, B B-complex 10g/kg, metronidazole 20g/kg, sucrose 20g/kg, agar 6g/kg, activated carbon 5g/kg stirs;
2. the branch base-material is trained in preparation: make base-material with humus soil, sawdust, perlite, each 200kg of decomposed rock, fully mix;
3. cut and pluck cutting: clip life in 2 years, diameter 0.5-1.0cm, growing way are healthy and strong, 2000 in undamaged branch, front 3 days of beta pruning, selected branch is carried out sterilizing with 1000 times of carbendazim dilutions, branch length 20-30cm, stay two joints, the bottom joint is from lower end 4-5cm, and top is saved from upper end 5-10cm; Tangent plane is level and smooth, and its upper end is flat mouth, and the lower end is the inclined-plane;
4. culture of rootage: will the branch lower end insert in the training branch nutrient solution and soaked 7 days, lower joint immerses, and upper joint outside;
5. seedbed arrangement: 40cm is ploughed deeply in the seedbed, and hack is raked, and is made into the furrow piece of wide 1m, holds the bar ditch successfully by line-spacing 20cm, with 10000 times of carbendazim dilutions sterilizing is carried out in the seedbed, will train the branch base-material and apply in the bar ditch thickness 8-10cm, a little compacting;
6. cuttage: the branch after will soaking carries out oblique cutting according to line-spacing 5cm, and lower joint will be imbedded in the training branch base-material, and compacting is watered;
7. seedling management: frequent water spray, keep ground moistening, loosen the soil, weeding; Young sprout grows and pinching when three pairs of leaves occurring, and again pinching when shoot grows three pairs of leaves again during height of seedling 30cm, is spread fertilizer over the fields each 1kg of urea and potassium dihydrogen phosphate, after growing seedlings 60 days, survives 1785 strains, and outplanting is transplanted.
Because adopted training branch nutrient solution to carry out culture of rootage, and adopted training branch base-material, so that the cuttage and seedling culture survival rate increases substantially, survival rate has reached 89%.。
Claims (1)
1. cuttage seedling culturing method for branch culture of honeysuckle comprises preparation training branch nutrient solution, preparation training branch base-material, cuts and pluck cutting, culture of rootage, seedbed arrangement, cuttage and seedling management totally seven large steps, it is characterized in that concrete grammar is as follows:
⑴ preparation training branch nutrient solution: with methyl α-naphthyl acetate 75mg/kg, salicylic acid 10g/kg, B B-complex 10g/kg, metronidazole 20g/kg, sucrose 20g/kg, agar 6g/kg, activated carbon 5g/kg stirs;
⑵ preparation training branch base-material: make base-material with equivalent humus soil, sawdust, perlite, decomposed rock, fully mix;
⑶ cut and pluck cutting: clip life in 2 years, diameter 0.5-1.0cm, healthy and strong, the undamaged branch of growing way; Before beta pruning 3-5 days, selected branch is carried out sterilizing with 1000 times of carbendazim dilutions, branch length 20-30cm stays two joints, and the bottom is saved from lower end 4-5cm, and top is saved from upper end 5-10cm; Tangent plane should be level and smooth, and its upper end is flat mouth, and the lower end is the inclined-plane;
⑷ culture of rootage: will soak 7 days in the branch lower end insertion training branch nutrient solution, lower joint immerses, and upper joint outside;
⑸ seedbed arrangement: 30-40cm is ploughed deeply in the seedbed, and hack is raked, and is made into the furrow piece of wide 1m, holds the bar ditch successfully by line-spacing 20cm, with 10000 times of carbendazim dilutions sterilizing is carried out in the seedbed, then will train the branch base-material and apply in the bar ditch thickness 8-10cm, a little compacting;
⑹ cuttage: the branch after will soaking carries out oblique cutting according to line-spacing 5cm, and lower joint will be imbedded in the training branch base-material, and compacting is watered;
⑺ seedling management: frequent water spray, keep ground moistening, loosen the soil, weeding; Young sprout grows and pinching when three pairs of leaves occurring, inspires shoot, and again pinching when shoot grows three pairs of leaves again during height of seedling 30cm, can suitably be applied fertilizer, and every mu is spread fertilizer over the fields urea and each 3kg of potassium dihydrogen phosphate, and after growing seedlings 60 days, nursery stock can go out the garden and transplant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201110292721 CN102405759B (en) | 2011-10-05 | 2011-10-05 | Cuttage seedling culturing method for branch culture of honeysuckle |
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| CN 201110292721 CN102405759B (en) | 2011-10-05 | 2011-10-05 | Cuttage seedling culturing method for branch culture of honeysuckle |
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| CN102405759A CN102405759A (en) | 2012-04-11 |
| CN102405759B true CN102405759B (en) | 2013-01-09 |
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Families Citing this family (21)
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|---|---|---|---|---|
| CN102696373A (en) * | 2012-05-29 | 2012-10-03 | 句容市白兔镇德明金银花专业合作社 | Cuttage seedling method for honey suckles |
| CN103535166A (en) * | 2012-07-12 | 2014-01-29 | 黄金利 | Lonicera japonica nursery stock rapid propagation method |
| CN103314748B (en) * | 2013-06-06 | 2015-01-14 | 山东省分析测试中心 | Seedling raising method of highbred honeysuckles |
| CN103598001B (en) * | 2013-11-29 | 2015-06-17 | 重庆市京百实业有限公司 | Method for raising honeysuckle seedling by cutting |
| CN103819236B (en) * | 2014-03-06 | 2016-04-06 | 西双版纳天源林石斛种植有限公司 | Fermented substrate and its production and use |
| CN103947402B (en) * | 2014-04-03 | 2016-07-06 | 遵义市易银种植有限公司 | A kind of cottage breeding method of Flos Lonicerae |
| CN104054481A (en) * | 2014-06-09 | 2014-09-24 | 卞佳林 | Cutting and seedling method of honeysuckle |
| CN104067798B (en) * | 2014-06-10 | 2016-06-22 | 长江大学 | The determination methods in cottage propagation gold silver flower seedling advantage root district |
| CN104170624B (en) * | 2014-08-15 | 2016-03-30 | 重庆德文金银花种植场 | A kind of honeysuckle cultivation method |
| CN105123211A (en) * | 2015-08-31 | 2015-12-09 | 杨胜成 | Honeysuckle planting method |
| CN105210636A (en) * | 2015-10-13 | 2016-01-06 | 广西乐业荣丰中药材科技有限责任公司 | A kind of wild Flos Lonicerae asexual reproduction method |
| CN105210784A (en) * | 2015-10-29 | 2016-01-06 | 广西乐业荣丰中药材科技有限责任公司 | A kind of vegetative propagation method of honeysuckle |
| CN105850482A (en) * | 2016-05-18 | 2016-08-17 | 高文靓 | Rhizoma atractylodis seedling cultivation method |
| CN106008033A (en) * | 2016-07-20 | 2016-10-12 | 宁夏共享生物化工有限公司 | Plant nutrient solution used in water culture and domestication period of flowers and plants and preparation method thereof |
| CN106962399B (en) * | 2017-03-22 | 2019-08-16 | 河南红枫种苗股份有限公司 | The cottage method of cuttage root-taking agent and descendants of royal families |
| CN107409717A (en) * | 2017-08-22 | 2017-12-01 | 张涛 | A kind of implantation methods of honeysuckle |
| CN108293454A (en) * | 2018-01-23 | 2018-07-20 | 广西正匠农业科技有限公司 | A kind of honeysuckle cuttage and seedling culture method |
| CN108575518A (en) * | 2018-05-25 | 2018-09-28 | 于明根 | The cultural method of Pu'er tea tree of area surroundings suitable for Zhejiang |
| CN108834521B (en) * | 2018-05-25 | 2020-08-25 | 于明根 | Pu 'er tea tree cultivation method for improving Pu' er tea yield and tea quality |
| CN109463388A (en) * | 2018-12-03 | 2019-03-15 | 上海农乐生物制品股份有限公司 | A kind of organic nitrogen source nutrient solution and its application |
| CN113647256A (en) * | 2021-07-22 | 2021-11-16 | 云南康养百年农业科技有限公司 | Cutting seedling raising method for honeysuckle |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748462A (en) * | 2005-10-20 | 2006-03-22 | 东北农业大学 | Green branch cuttage breeding method for indigo fruit honeysuckle |
| CN1820585A (en) * | 2006-03-23 | 2006-08-23 | 刘海涛 | High efficiency breeding technology for lonicera macranthoides honeysuckle |
| CN101595802B (en) * | 2009-07-09 | 2011-01-05 | 四川省农业科学院经济作物育种栽培研究所 | Method for cutting lonicera similis hemsl |
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