CN1820585A - High efficiency breeding technology for lonicera macranthoides honeysuckle - Google Patents
High efficiency breeding technology for lonicera macranthoides honeysuckle Download PDFInfo
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- CN1820585A CN1820585A CN 200610050985 CN200610050985A CN1820585A CN 1820585 A CN1820585 A CN 1820585A CN 200610050985 CN200610050985 CN 200610050985 CN 200610050985 A CN200610050985 A CN 200610050985A CN 1820585 A CN1820585 A CN 1820585A
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Abstract
The present invention relates to high efficiency breeding method of Lonicera macranthoides honeysuckle, and belongs to asexual plant breeding method. The technological scheme includes the steps of sterilization of the branch picking garden, killing pest and sterilizing in the seedbed, picking branch, cutting, planting, seedbed management and temporary planting. In the seedbed management, the activation stage and the healing stage have humidity controlled in 60-80 %, ground temperature controlled in 25-28deg.c and house temperature controlled in 15-30deg.c. The time from branch picking to planting is controlled within 2 hr. The method of the present invention has survival rate up to 70 % and low cost.
Description
Technical field: the present invention relates to the asexual reproduction method of a plant species, relate in particular to a kind of method with cottage method breeding lonicera macranthoides honeysuckle.
Background technology: honeysuckle belongs to the bud of caprifoliaceae plant honeysuckle Lonicera japonica Thunb, has clearing heat and detoxicating, sterilization, heat-clearing, the effect of hepatic cholagogic.Lonicera macranthoides honeysuckle is the improved seeds of honeysuckle, and it has characteristics such as many, the effective component content height of blooming; At present, adopt traditional cottage method to breed largeflower-like honeysuckle flower, its survival rate has only about 5%, can not satisfy the needs of large-scale production, therefore generally all is to adopt the mode of grafting or tissue propagation to grow seedlings.Advantages such as plant adaptability is strong though propagation by grafiting has, well developed root system, life-span length need a large amount of collection wild resources, easily wild resource is caused large-area destruction, and operation are loaded down with trivial details, survival rate also has only 10～20%; And tissue propagation utilizes the histoorgan of plant to breed, and mainly has defectives such as cost height, operation be numerous and diverse.
Summary of the invention: at the above-mentioned defective that exists in the prior art, the present invention aims to provide a kind of seedling percent height, low, the simple high efficiency breeding technology for lonicera macranthoides honeysuckle of technology of cost.
Technical scheme of the present invention comprises adopts a garden sterilization, deinsectization and nursery bed disinfection, sterilization, adopts branch, beta pruning, cuttage, seedbed management and heeling in; The concrete operations step is as follows:
(1) adopts a garden sterilization, deinsectization and nursery bed disinfection, sterilization
1. adopted branch preceding 7～10 days, with concentration is that 50% carbendazim is diluted to 500 times the aqueous solution, the aqueous solution that hot sulphur is made into 500 times carries out sterilization to adopting a garden, deinsectization is handled, and potassium permanganate is made into concentration is 0.1% the aqueous solution, be that to be made into concentration be 4g/m for 50% carbendazim with concentration
3The aqueous solution to the seedbed carry out disinfection, sterilization processing;
(2) adopting branch selects the plant of life in 1～2 year to adopt branch;
(3) it is that 40～50 ° of inclined-planes, length are the branch of 10～15cm for flat mouth, lower end that the plant under beta pruning will be adopted cuts into the upper end, and the tangent plane of two-port should be level and smooth, and is phozy;
(4) cuttage the seedbed is watered permeable, then with branch cutting in the matrix in seedbed, compress branch and keep blade moistening; Ground temperature should be controlled at 15～25 ℃, room temperature and was controlled at 20～25 ℃ this moment;
(5) seedbed management
1. the pot-life time is 7～10 days, and relative moisture is controlled at 60～80% therebetween, and ground temperature is controlled at 25～28 ℃, room temperature and is controlled at 15～30 ℃;
2. time callus phase is 14～16 days, and relative moisture is controlled at 60～80% therebetween, and ground temperature is controlled at 25～28 ℃, room temperature and is controlled at 18～30 ℃;
3. practicing the time in seedling stage is 8～10 days, and water 1～2 preceding 4～5 day every day, opens Miao Peng skylight and side window noon every day and ventilates 2～3 hours; The back stopped to water in 4～5 days, and whole day opens the Miao Peng skylight and side window keeps ventilating;
(6) heel in finish in experienced seedling stage after, to big Tanaka, be that desirable seedling is sold with the root plantlet of transplant that to have grown 3～5 length be 3～5cm adventive root through 8～10 months heel in.
Pot-life and the best room temperature of callus phase, ground temperature are 30 ℃, 26 ℃ respectively in the step (5), and best relative moisture is 80%; Plant should be controlled in 2 hours in the time on the seedbed to cuttage from adopting branch.
Should be noted that: in order to guarantee the effect of sterilization, deinsectization and sterilization, step (1) is preferably in fine day and treats to carry out when dew on the plant is done.Step (2) is preferably in carries out morning, the evaporation, air-dry as yet of the dew on the plant at this moment, and blade compares comparatively fresh; Should deposit in cool place, lee side after branch is adopted and sheared off and preserve, and water spray keeps the fresh moistening survival rate that is beneficial to improve after the cuttage of blade; Should select stalwartness when adopting, enrich, the plant of no damage by disease and insect, the branch with the semi-lignified of just taking out the tip is good especially.Step (4) is generally carried out to good in spring, two seasons of autumn, and plant cuttage spacing is advisable with 5 * 4cm; If the matrix in seedbed is perlite, then the cuttage degree of depth of plant is generally 3～5cm; If the matrix in seedbed is perlite and the mixing of detritus soil, then the cuttage degree of depth of plant is generally 3cm; Should be that horseshoe-shaped inclined-plane inserts in the matrix with the lower end during cuttage, can not insert, and note keeping blade moistening.In the step (5), should water at any time to the seedling of callus phase, water about 14 times general every day.
Compared with the prior art, the present invention helps seedling activation, callus and practices environment such as the required temperature of seedling, humidity owing to being provided with in the seedbed management phase, thereby has significantly improved the survival rate of seedling, and seedling percent can reach more than 70%; Cost also is reduced to 0.8 yuan of average every strain from original average every strain more than 2 yuan, can save seedling cost about 60%, has effectively overcome the defective that largeflower-like honeysuckle flower tradition propagation method survival rate is low, seedling cost is high.
Embodiment: the invention will be further described below in conjunction with specific embodiment:
Embodiment 1, and operating procedure is as follows:
(1) adopts a garden sterilization, deinsectization and nursery bed disinfection, sterilization
1. adopted branch preceding 7 days, with concentration is that 50% carbendazim is diluted to 500 times the aqueous solution, the aqueous solution that hot sulphur is made into 500 times carries out sterilization to adopting a garden, deinsectization is handled, and potassium permanganate is made into concentration is 0.1% the aqueous solution, be that to be made into concentration be 4g/m for 50% carbendazim with concentration
3The aqueous solution to the seedbed carry out disinfection, sterilization processing;
(2) adopting branch selects the stalwartness of life in 2 years, the plant of no damage by disease and insect to adopt branch;
(3) it is that 45 ° of inclined-planes, length are the branch of 15cm for flat mouth, lower end that the plant under beta pruning will be adopted cuts into the upper end, and the tangent plane of two-port should be level and smooth, and is phozy;
(4) cuttage the seedbed is watered permeable, again with the lower end cuttage of branch in the matrix in seedbed, compress branch and keep blade moistening; Ground temperature should be controlled at 18 ℃, room temperature and was controlled at 22 ℃ this moment;
(5) seedbed management
1. the pot-life time is 7 days, and relative moisture is controlled at 60% therebetween, and ground temperature is controlled at 25 ℃, room temperature and is controlled at 15 ℃;
2. time callus phase is 15 days, and relative moisture is controlled at 60% therebetween, and ground temperature is controlled at 25 ℃, room temperature and is controlled at 15 ℃;
3. practicing the time in seedling stage is 8 days, and water 2 preceding 4 day every day, opens Miao Peng skylight and side window noon every day and ventilates 3 hours; The back stopped to water in 4 days, and whole day opens the Miao Peng skylight and side window keeps ventilating;
(6) heel in finish in experienced seedling stage after, to big Tanaka, be that desirable seedling is sold with the root plantlet of transplant that to have grown 3～5 length be 3～5cm adventive root through 8 months heel in.
Embodiment 2: repeat the step of embodiment 1, wherein in the step (5), pot-life, the relative moisture of callus phase all are controlled at 80%, and ground temperature, room temperature all are controlled at 28 ℃ and 30 ℃.
Embodiment 3: repeat the step of embodiment 1, wherein in the step (5), pot-life, the relative moisture of callus phase all are controlled at 80%, and ground temperature, room temperature all are controlled at 26 ℃ and 30 ℃.
In the above-described embodiments, plant should be controlled to the time of cuttage on the seedbed and finished with interior in 2 hours from adopting branch.
Claims (3)
1. a high efficiency breeding technology for lonicera macranthoides honeysuckle comprises and adopts a garden sterilization, deinsectization and nursery bed disinfection, sterilization, and adopts branch, beta pruning, cuttage, seedbed management, heels in; It is characterized in that: operating procedure is as follows:
(1) adopts a garden sterilization, deinsectization and nursery bed disinfection, sterilization
1. adopted branch preceding 7～10 days, with concentration is that 50% carbendazim is diluted to 500 times the aqueous solution, the aqueous solution that hot sulphur is made into 500 times carries out sterilization to adopting a garden, deinsectization is handled, and potassium permanganate is made into concentration is 0.1% the aqueous solution, be that to be made into concentration be 4g/m for 50% carbendazim with concentration
3The aqueous solution to the seedbed carry out disinfection, sterilization processing;
(2) adopting branch selects the plant of life in 1～2 year to adopt branch;
(3) it is that 40～50 ° of inclined-planes, length are the branch of 10～15cm for flat mouth, lower end that the plant under beta pruning will be adopted cuts into the upper end, and the tangent plane of two-port should be level and smooth, and is phozy;
(4) cuttage the seedbed is watered permeable, then with branch cutting in the matrix in seedbed, compress branch and keep blade moistening; Ground temperature should be controlled at 15～25 ℃, room temperature and was controlled at 20～25 ℃ this moment;
(5) seedbed management
1. the pot-life time is 7～10 days, and relative moisture is controlled at 60～80% therebetween, and ground temperature is controlled at 25～28 ℃, room temperature and is controlled at 15～30 ℃;
2. time callus phase is 14～16 days, and relative moisture is controlled at 60～80% therebetween, and ground temperature is controlled at 25～28 ℃, room temperature and is controlled at 18～30 ℃;
3. practicing the time in seedling stage is 8～10 days, and water 1～2 preceding 4～5 day every day, opens Miao Peng skylight and side window noon every day and ventilates 2～3 hours; The back stopped to water in 4～5 days, and whole day opens the Miao Peng skylight and side window keeps ventilating;
(6) heel in finish in experienced seedling stage after, with root grown 3～5, length be the plantlet of transplant of 3～5cm adventive root to big Tanaka, be that desirable seedling is sold through 8～10 months heel in.
2. high efficiency breeding technology for lonicera macranthoides honeysuckle according to claim 1 is characterized in that: pot-life and the room temperature of callus phase, ground temperature are 30 ℃, 26 ℃ respectively in the step (5), and relative moisture is 80%.
3. high efficiency breeding technology for lonicera macranthoides honeysuckle according to claim 1 and 2 is characterized in that: plant should be controlled in 2 hours in the time on the seedbed to cuttage from adopting branch.
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200610050985 CN1820585A (en) | 2006-03-23 | 2006-03-23 | High efficiency breeding technology for lonicera macranthoides honeysuckle |
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| CN 200610050985 CN1820585A (en) | 2006-03-23 | 2006-03-23 | High efficiency breeding technology for lonicera macranthoides honeysuckle |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405759A (en) * | 2011-10-05 | 2012-04-11 | 重庆市秀山红星中药材开发有限公司 | Cuttage seedling culturing method for branch culture of honeysuckle |
| CN102415333A (en) * | 2011-10-16 | 2012-04-18 | 重庆市秀山红星中药材开发有限公司 | Breeding method for lonicera macranthoides honeysuckle early budding varieties |
| CN102440134A (en) * | 2011-10-16 | 2012-05-09 | 重庆市秀山红星中药材开发有限公司 | High-yield honeysuckle cultivation method |
| CN102577969A (en) * | 2012-03-08 | 2012-07-18 | 重庆文理学院 | Breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1 |
| CN102612889A (en) * | 2012-04-09 | 2012-08-01 | 常熟市海虹苗木花卉有限公司 | Method for increasing seedling survival rate of Lonicera sempervirens seeds |
| CN102657021A (en) * | 2012-05-31 | 2012-09-12 | 山东潍坊东盛园艺有限公司 | Mist cutting cultivation method using blueleaf honeysuckle shoots |
| CN102657082A (en) * | 2012-04-28 | 2012-09-12 | 湖南农业大学 | In-vitro culture and planting regeneration and propagation method of Xianglei honeysuckle leaves and culture medium |
| CN103314748A (en) * | 2013-06-06 | 2013-09-25 | 山东省分析测试中心 | Seedling raising method of highbred honeysuckles |
| CN103535166A (en) * | 2012-07-12 | 2014-01-29 | 黄金利 | Lonicera japonica nursery stock rapid propagation method |
| CN103947402A (en) * | 2014-04-03 | 2014-07-30 | 遵义市易银种植有限公司 | Cuttage cultivation method of honeysuckles |
| CN103988774A (en) * | 2014-05-27 | 2014-08-20 | 贵定县隆达金银花科技发展有限公司 | Method for hybridizing and breeding medicinal honeysuckle |
| CN104186194A (en) * | 2014-09-27 | 2014-12-10 | 管天球 | Method for cultivating honeysuckle in rocky land |
| CN104380939A (en) * | 2014-09-16 | 2015-03-04 | 南江县百草中药材有限公司 | Honeysuckle flower planting method |
| CN104885724A (en) * | 2015-03-02 | 2015-09-09 | 杨文成 | High-yield honeysuckle cultivation method |
| CN105230315A (en) * | 2015-11-06 | 2016-01-13 | 广西大海阳光药业有限公司 | Rapid flos lonicerae propagation method |
| CN105900622A (en) * | 2016-04-13 | 2016-08-31 | 湖南省林业科学院 | Twig cutting seedling breeding method for lonicera macranthoides |
| CN106386489A (en) * | 2016-09-12 | 2017-02-15 | 北华大学 | Tissue culture and rapid propagation method of lonicera ruprechtiana |
-
2006
- 2006-03-23 CN CN 200610050985 patent/CN1820585A/en active Pending
Cited By (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102405759A (en) * | 2011-10-05 | 2012-04-11 | 重庆市秀山红星中药材开发有限公司 | Cuttage seedling culturing method for branch culture of honeysuckle |
| CN102415333A (en) * | 2011-10-16 | 2012-04-18 | 重庆市秀山红星中药材开发有限公司 | Breeding method for lonicera macranthoides honeysuckle early budding varieties |
| CN102440134A (en) * | 2011-10-16 | 2012-05-09 | 重庆市秀山红星中药材开发有限公司 | High-yield honeysuckle cultivation method |
| CN102577969A (en) * | 2012-03-08 | 2012-07-18 | 重庆文理学院 | Breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1 |
| CN102612889B (en) * | 2012-04-09 | 2014-09-10 | 苏州瑞邦塑胶有限公司 | Method for increasing seedling survival rate of Lonicera sempervirens seeds |
| CN102612889A (en) * | 2012-04-09 | 2012-08-01 | 常熟市海虹苗木花卉有限公司 | Method for increasing seedling survival rate of Lonicera sempervirens seeds |
| CN102657082A (en) * | 2012-04-28 | 2012-09-12 | 湖南农业大学 | In-vitro culture and planting regeneration and propagation method of Xianglei honeysuckle leaves and culture medium |
| CN102657021A (en) * | 2012-05-31 | 2012-09-12 | 山东潍坊东盛园艺有限公司 | Mist cutting cultivation method using blueleaf honeysuckle shoots |
| CN102657021B (en) * | 2012-05-31 | 2014-01-08 | 山东潍坊东盛园艺有限公司 | Mist cutting cultivation method using blueleaf honeysuckle shoots |
| CN103535166A (en) * | 2012-07-12 | 2014-01-29 | 黄金利 | Lonicera japonica nursery stock rapid propagation method |
| CN103314748A (en) * | 2013-06-06 | 2013-09-25 | 山东省分析测试中心 | Seedling raising method of highbred honeysuckles |
| CN103314748B (en) * | 2013-06-06 | 2015-01-14 | 山东省分析测试中心 | Seedling raising method of highbred honeysuckles |
| CN103947402A (en) * | 2014-04-03 | 2014-07-30 | 遵义市易银种植有限公司 | Cuttage cultivation method of honeysuckles |
| CN103947402B (en) * | 2014-04-03 | 2016-07-06 | 遵义市易银种植有限公司 | A kind of cottage breeding method of Flos Lonicerae |
| CN103988774A (en) * | 2014-05-27 | 2014-08-20 | 贵定县隆达金银花科技发展有限公司 | Method for hybridizing and breeding medicinal honeysuckle |
| CN104380939A (en) * | 2014-09-16 | 2015-03-04 | 南江县百草中药材有限公司 | Honeysuckle flower planting method |
| CN104186194A (en) * | 2014-09-27 | 2014-12-10 | 管天球 | Method for cultivating honeysuckle in rocky land |
| CN104186194B (en) * | 2014-09-27 | 2016-02-10 | 管天球 | A kind ofly cultivate the method for honeysuckle in three difficulties |
| CN104885724A (en) * | 2015-03-02 | 2015-09-09 | 杨文成 | High-yield honeysuckle cultivation method |
| CN105230315A (en) * | 2015-11-06 | 2016-01-13 | 广西大海阳光药业有限公司 | Rapid flos lonicerae propagation method |
| CN105900622A (en) * | 2016-04-13 | 2016-08-31 | 湖南省林业科学院 | Twig cutting seedling breeding method for lonicera macranthoides |
| CN106386489A (en) * | 2016-09-12 | 2017-02-15 | 北华大学 | Tissue culture and rapid propagation method of lonicera ruprechtiana |
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