CN103988774A - Method for hybridizing and breeding medicinal honeysuckle - Google Patents
Method for hybridizing and breeding medicinal honeysuckle Download PDFInfo
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- CN103988774A CN103988774A CN201410228249.8A CN201410228249A CN103988774A CN 103988774 A CN103988774 A CN 103988774A CN 201410228249 A CN201410228249 A CN 201410228249A CN 103988774 A CN103988774 A CN 103988774A
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Abstract
The invention discloses a method for hybridizing and breeding medicinal honeysuckle and belongs to the field of Chinese herbal medicine hybridizing and breeding. The method comprises the following steps: (1) selecting a parent; (2) hybridizing to obtain seeds; (3) seeding; (4) transplanting; (5) snagging; (6) roguing; (7) breeding, and establishing a strain; (8) breeding; and (9) planting and performing batch reproduction. The method has the beneficial effects that with the adoption of the medicinal honeysuckle bred through the steps, the defect that a female parent is easily infected by local diseases during plantation in Guizhou is overcome, the insect resistance and drought resistance on local insects are enhanced, and the management cost in the planting process is reduced. Moreover, the yield of the medicinal honeysuckle and the content of main medicinal ingredient chlorogenic acid in the honeysuckle are extremely high, and the economic benefits are greatly improved.
Description
Technical field
The present invention relates to Chinese herbal medicine selection cross field, be specifically related to a kind of medicinal gold honeysuckle flower selection cross method.
Background technology
Honeysuckle is a kind of conventional Chinese medicine with long history, begins to be loaded in < < Mingyi Bielu > >, classifies as top grade." honeysuckle " one is first appeared in Li Shizhen (1518-1593 A.D.) < < Compendium of Materia Medica > >, under " honeysuckle " item, mention, because Modern Document is in use for a long time, now be known as the rectification of name of this medicinal material, and taken in < < Chinese pharmacopoeia > >.Honeysuckle is just extensive and famous with its medical value since ancient times, and its effect is mainly clearing heat and detoxicating, cures mainly warm disease heating, toxic-heat and blood stasis, ulcer serious case of furuncle etc.Modern study proves, honeysuckle contains the pharmacological components such as chlorogenic acid, cyanidenon glycosides, the various pathogens such as hemolytic streptococcus, Staphylococcus aureus and infection of the upper respiratory tract Causative virus etc. are had to stronger restraint, also can strengthen in addition immunity, anti-early pregnancy, protect liver, antitumor, anti-inflammatory, antipyretic, hemostasis (blood coagulation), suppress intestinal absorption cholesterol etc., its clinical application is very extensive, can be used for the treatment of respiratory tract infection, bacillary dysentery, acute urinary, hypertension etc. more than 40 with other medicines compatibility and plant illness.China is as not lower 17 kinds of the former plant total of honeysuckle (comprising subspecies and mutation) of commodity selling, and the widest with this kind of distribution, sales volume is also maximum.At present, the state between supply and demand of honeysuckle is: the yearly productive capacity of national honeysuckle is always in 5,000,000 kg left and right, and actual demand amount international, domestic market is at least 2,000 ten thousand kg, and demand is also in continuous increase, because of medicinal material, supply falls short of demand, introducing and planting is just being actively developed in many areas, and the producing region of honeysuckle day by day expands.
Periodical < < Pharmaceutical Biotechnology > > the 5th periodical in 2008 has carried the induction > > [J] of document < < honeysuckle inter breed crossing and allotetraploid, the document discloses a kind of honeysuckle selection cross method, it is male parent that the method be take Shandong pan honeysuckle flower, Henan pan honeysuckle flower is that female parent carries out artificial hybridization breeding, by peroxide (POD) Isozyme Analysis, identify the hybridity of the hybrid seed of collecting, pick out the filial generation with parent's shape.This kind of honeysuckle selection cross method is that to take Shandong pan honeysuckle flower and Henan pan honeysuckle flower be parent, the area that the offspring that selection cross goes out is applicable to plantation is confined to Shandong and Henan area, be not suitable for other areas, a little less than other regional insect pest disease resistivities, particularly also there is the Guizhou of " congealing " weather; And the hybridity of the method employing peroxide (POD) Isozyme Analysis evaluation hybrid seed, also hybrid seed to be carried out to mutagenesis and just can obtain tetraploid honeysuckle, could obtain more large-tonnage hybrid seed, process complexity and cost are higher.
Summary of the invention
For above the deficiencies in the prior art, the inventor has carried out a large amount of deep research, after having paid performing creative labour, thereby has completed the present invention.
The invention provides a kind of medicinal gold honeysuckle flower selection cross method, the crossbreed selecting can significantly be improved the maternal pest-resistant disease-resistant and drought-resistant ability in Guizhou province, promoted the resistance to leaf blight, anthracnose, rust staining disease, brown spot, and the content of the output of its medicinal gold honeysuckle flower and medicinal flos lonicerae use composition chlorogenic acid is also very high, has strengthened its viablity under the weather of Guizhou peculiar " congealing ".
The technical solution used in the present invention is a kind of medicinal gold honeysuckle flower selection cross method, comprise the following steps: (1) Selection parent, take Shandong tetraploid honeysuckle as maternal, guarantee the high yield of Hybrid, Guizhou hairy honeysuckle is male parent, guarantees the high-load of Chlorogenic Acid of Flos Lonicerae that Hybrid produces; (2) hybridization obtains kind, guiding county county corrie choose a farmland as seed selection seedling field, by artificial pollination mode, obtain medicinal gold honeysuckle flower hybrid seed; (3) sowing; The seeding quantity in every mu of seedling field is 0.600～0.800kg; (4) transplant; (5) disconnected branch; (6) roguing, by observing, picks out that the relative parent of coring is long, short, the colored or large plant of the relative parent of bud of the relative parent of interval on branch; (7) breed, set up strain; (8) seed selection, the pest-resistant disease-resistant and drought resistance of the content of medicinal Chlorogenic Acid of Flos Lonicerae, the output of medicinal gold honeysuckle flower and plant of take is carried out seed selection to plant as standard; (9) fixed kind, and breeding in batches.
As preferably, for the preservation of hybrid seed, in described step (2), the hybrid seed that obtains is sowed to next year be kept at gunnysack at 0～5 ℃ of temperature in.
As preferably, for the germination to hybrid seed and growth provide required fertilizer and a healthy growing environment, seedling field in described step (3) prior to seeding, every mu adds 600～800kg fertilizer, use again the liquor potassic permanganate that 0.25～0.35kg95% fenaminosulf soluble powder (the 95% fenaminosulf soluble powder meaning is in this disinfectant, and the mass ratio of fenaminosulf is 5%) or 45～55g mass ratio are 0.5% to kill virus.
As preferably, in order to shorten the cultivation time, described seed first passes through vernalization, then sows.
As preferably, in order to make the hybrid seed can be with the fastest mode rudiment, the vernalization mode of hybrid seed be, first hybrid seed is placed in 25～35 ℃ of warm water and soaks 24 hours, then at room temperature puddles with wet sand, makes the hybrid seed can be with the fastest mode rudiment.
As preferably, in order to utilize best seedling field and to be that germination and the growth of seed provides best environment, the seeding quantity on described every mu of seedling field is 0.6300～0.6800kg.
As preferably, in order to ensure seedling strain, after transplanting, can survive and growth healthily, the seedling strain length of being transplanted in described step (4) is 15～20cm, and is balled transplanting.
As preferably, when continuing healthy growth in order to ensure seedling strain after disconnected branch, impel to greatest extent its branch, in described step (5), by the seedling strain length of disconnected branch, be 30～40cm.
As preferably, constant in order to guarantee the proterties of educated plant, the mode of reproduction in described step (7) is that cuttage or press strip are bred.
Beneficial effect of the present invention is: by the medicinal gold honeysuckle flower that adopts above step to cultivate, not only possessed male parent Guizhou hair and born the pest-resistant disease-resistant and drought-resistance ability of east under the high height above sea level in Guizhou, widow's Special Geographic weather at sunshine, reduced artificial planting management cost, the advantage that has also possessed maternal Shandong tetraploid honeysuckle high yield, and the content of contained medicinal component chlorogenic acid is also very high, the viablity that has strengthened peculiar in Guizhou " congealing " weather, has promoted its economic benefit greatly.
Embodiment
For making those skilled in the art understand in detail production technology of the present invention and beneficial effect, with concrete production instance, further introduce application of the present invention and beneficial effect below.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment mono-
Guiding county county corrie choose a farmland as seed selection seedling field, select Shandong tetraploid honeysuckle as female parent, Guizhou hair is born east as male parent, in early or mid May, carry out artificial pollination, in August from robust growth, collect ripe and have the honeysuckle hybrid seed of obvious difference with parental seed shape and color without the plant of damage by disease and insect or branch, by after Seed cleaning up, at 0～5 ℃ of temperature, aeration-drying is preserved.Seed first passes through vernalization prior to seeding, then sows, and the sowing time is 2～March; The seedling field of sowing first adds appropriate fertilizer, and keeps suitable moisture and illumination, and kills virus with fenaminosulf, and the seeding quantity on seedbed is 0.60000kg/ mu; After emerging, when 15～20cm length is grown in seedling strain, transplant; When 30～40cm is grown in seedling strain, the point that breaks, impels branch.Observe root, branch, leaf, flower bud, the flower of plant, select the plant that there is obvious difference with parent and carry out cutting propagation, set up strain.Take select strain as seed selection object, and the pest-resistant disease-resistant and drought resistance of the content of medicinal Chlorogenic Acid of Flos Lonicerae and galuteolin, the output of medicinal gold honeysuckle flower and plant of take is carried out seed selection to strain as standard.Through above-mentioned Breeding Process, determine that medicinal gold honeysuckle flower output is high, medicinal component content is large and the strain of pest-resistant disease-resistant drought resisting, and it is carried out to batch breeding.
Final determined medicinal gold honeysuckle flower kind, in planting in Guizhou, is entered to high yield period about 3 years.Compare with parent, enter high yield period after per mu yield can reach dried flower 135～155kg, and high yield period can continuous 10～13 years, the contained chlorogenic acid mass-energy density of unit medicinal gold honeysuckle flower reaches 5.5%～6%.Obviously meet < < Pharmacopoeia of People's Republic of China > > (version in 2010) regulation.
2009 areal, Nian guiding county counties have chosen that three sizes are the same, the much the same seedling of natural environment field, have planted respectively Shandong tetraploid honeysuckle, Guizhou hairy honeysuckle and institute and have obtained hybridization honeysuckle kind.Since 2010, collect respectively the honeysuckle to 2013 year in three seedling fields, and carry out the mensuration of the content of output and the contained medicinal component chlorogenic acid of unit honeysuckle.Measure referring to table 1 in detail.
Table 1
Big or small 8 identical farmlands are chosen as seedling field in areal, corrie place, Guiding County, Zai Guizhou Province, and to seedling field label 1～8, in 8 seedling fields, add the fertilizer of equivalent and kill virus with the fenaminosulf of equivalent, then in plantation Shandong, 8 seedling fields tetraploid honeysuckle, Guizhou hairy honeysuckle and institute, obtaining each 100 strains of hybridization honeysuckle kind.According to these 8 seedling fields of identical plantation workflow management after one month, Chinese honeysuckle rounded tail aphid, honeysuckle looper (popular name is crossed insect), aphid, longicorn, looper, the honeysuckle brown spot pathogene and powdery mildew pathogene of Guizhou province is thrown in the seedling field that is 1～7 at label successively, and the damage by disease and insect of any Guizhou province is not thrown in seedling field 8.After one month, observe survival volume or the healthy amount of three kinds of kind honeysuckle plant in 8 farmlands, and contrast.Contrast is referring to table 2 in detail.
Table 2
By table 1 and table 2, can find out, the medicinal gold honeysuckle flower plant that adopts above step to cultivate, not only possessed male parent Guizhou hair and born the pest-resistant disease-resistant and drought-resistance ability of east under the high height above sea level in Guizhou, widow's Special Geographic weather at sunshine, superpower viability under the weather of Guizhou peculiar " congealing ", obviously reduced artificial planting management cost, the advantage that has also possessed maternal Shandong tetraploid honeysuckle high yield, and the also raising greatly of the content of contained medicinal component chlorogenic acid, greatly promoted its economic benefit.
Embodiment bis-:
Guiding county county corrie choose a farmland as seed selection seedling field, select Shandong tetraploid honeysuckle as female parent, Guizhou hair is born east as male parent, in early or mid May, carry out artificial pollination, in October from robust growth, collect ripe and have the honeysuckle hybrid seed of obvious difference with parental seed in shape and color without the plant of damage by disease and insect or branch, by after Seed cleaning up, at 0～5 ℃ of temperature, be kept at gunnysack in to next year sowing, the sowing time is 2～March.Seed is first placed on prior to seeding in 28 ℃ of warm water and soaks 24 hours, then at room temperature puddles vernalization with wet sand, when 30～40% seed breach, then sows; It is 650kg that every mu, the seedling field of sowing adds fertilizer, then with 400 times of dilutions of 0.35kg95% fenaminosulf soluble powder, kills virus for every mu, and the seeding quantity on seedbed is 0.7000kg/ mu; After emerging, when 15～20cm length is grown in seedling strain, transplant; When 30～40cm is grown in seedling strain, the point that breaks, impels branch.Observe root, branch, leaf, flower bud, the flower of plant, select that the relative parent of coring is long, on branch the relative parent of interval short, spend or the relative parent of bud greatly and blade and parent have the plant of obvious difference to carry out press strip to breed, and set up strain.Take select strain as seed selection object, and wherein, the long plant of coring carries out drought resisting seed selection, is planted in the few place of the water yield, observes its growth conditions; On branch interval short, flower or the large plant of bud carry out the seed selection of medicinal gold honeysuckle flower output and medicinal gold honeysuckle flower medicinal component chlorogenic acid and galuteolin content; Blade and parent have the strain of obvious difference to carry out pest-resistant disease-resistant seed selection, and the insect in Guizhou and leaf blight, anthracnose, rust staining disease, brown spot pathogene are acted directly on seed selection object, observe its pest-resistant disease resistance.Through above-mentioned Breeding Process, determine that medicinal gold honeysuckle flower output is high, medicinal component content is large and the strain of pest-resistant disease-resistant drought resisting, and it is carried out to batch breeding.
Final determined medicinal gold honeysuckle flower kind, in planting in Guizhou, is entered to high yield period about 3 years.Compare with parent, enter high yield period after per mu yield can reach dried flower 150～170kg, and high yield period can continuous 12～14 years, the contained chlorogenic acid mass-energy density of unit medicinal gold honeysuckle flower reaches 5.5%～6%.Obviously meet < < Pharmacopoeia of People's Republic of China > > (version in 2010) regulation.
2009 areal, Nian guiding county counties have chosen that three sizes are the same, the much the same seedling of natural environment field, and plant respectively Shandong tetraploid honeysuckle, Guizhou hairy honeysuckle and institute and obtain hybridization honeysuckle kind, and according to general plantation workflow management.Since 2010, collect respectively the honeysuckle to 2013 year in three seedling fields, and carry out the mensuration of the content of output and the contained medicinal component chlorogenic acid of unit honeysuckle.Measure referring to table 3 in detail.
Table 3
By table 2 and table 3, can find out, the medicinal gold honeysuckle flower plant that adopts above step to cultivate, not only possessed male parent Guizhou hair and born the pest-resistant disease-resistant and drought-resistance ability of east under the high height above sea level in Guizhou, widow's Special Geographic weather at sunshine, superpower viability under the weather of Guizhou peculiar " congealing ", obviously reduced artificial planting management cost, the advantage that has also possessed maternal Shandong tetraploid honeysuckle high yield, and the also raising greatly of the content of contained medicinal component chlorogenic acid, greatly promoted its economic benefit.
Embodiment tri-:
Guiding county county corrie choose a farmland as seed selection seedling field, select Shandong tetraploid honeysuckle as female parent, Guizhou hair is born east as male parent, in early or mid May, carry out artificial pollination, in September from robust growth, collect ripe and have the honeysuckle hybrid seed of obvious difference with parental seed in shape and color without the plant of damage by disease and insect or branch, by after Seed cleaning up, at 0～5 ℃ of temperature, be kept at gunnysack in to next year sowing, the sowing time is 2～March.Seed is first placed on prior to seeding in 30 ℃ of warm water and soaks 24 hours, then at room temperature puddles vernalization with wet sand, when 30～40% seed breach, then sows; It is 750kg that every mu, the seedling field of sowing adds fertilizer, then with 400 times of dilutions of 0.30kg95% fenaminosulf soluble powder, kills virus for every mu, and the seeding quantity on seedbed is 0.6667kg/ mu; After emerging, when 15～20cm length is grown in seedling strain, transplant; When 30～40cm is grown in seedling strain, the point that breaks, impels branch.Observe root, branch, leaf, flower bud, the flower of plant, select that the relative parent of coring is long, on branch the relative parent of interval short, spend or the relative parent of bud greatly and blade and parent have the plant of obvious difference to carry out press strip to breed, and set up strain.Take select strain as seed selection object, and wherein, the long plant of coring carries out drought resisting seed selection, is planted in the few place of the water yield, observes its growth conditions; On branch interval short, flower or the large plant of bud carry out the seed selection of medicinal gold honeysuckle flower output and medicinal gold honeysuckle flower medicinal component chlorogenic acid and galuteolin content; Blade and parent have the strain of obvious difference to carry out pest-resistant disease-resistant seed selection, and the insect in Guizhou and leaf blight, anthracnose, rust staining disease, brown spot pathogene are acted directly on seed selection object, observe its pest-resistant disease resistance.Through above-mentioned Breeding Process, determine that medicinal gold honeysuckle flower output is high, medicinal component content is large and the strain of pest-resistant disease-resistant drought resisting, and it is carried out to batch breeding.
Final determined medicinal gold honeysuckle flower kind, in planting in Guizhou, is entered to high yield period about 3 years.Compare with parent, enter high yield period after per mu yield can reach dried flower 150～170kg, and high yield period can continuous 12～14 years, the contained chlorogenic acid mass-energy density of unit medicinal gold honeysuckle flower reaches 5.8%～6.2%.Obviously meet < < Pharmacopoeia of People's Republic of China > > (version in 2010) regulation.
2009 areal, Nian guiding county counties have chosen that three sizes are the same, the much the same seedling of natural environment field, and plant respectively Shandong tetraploid honeysuckle, Guizhou hairy honeysuckle and institute and obtain hybridization honeysuckle kind, and according to general plantation workflow management.Since 2010, collect respectively the honeysuckle to 2013 year in three seedling fields, and carry out the mensuration of the content of output and the contained medicinal component chlorogenic acid of unit honeysuckle.Measure referring to table 4 in detail.
Table 4
By table 2 and table 4, can find out, the medicinal gold honeysuckle flower plant that adopts above step to cultivate, not only possessed male parent Guizhou hair and born the pest-resistant disease-resistant and drought-resistance ability of east under the high height above sea level in Guizhou, widow's Special Geographic weather at sunshine, superpower viability under the weather of Guizhou peculiar " congealing ", obviously reduced artificial planting management cost, the advantage that has also possessed maternal Shandong tetraploid honeysuckle high yield, and the also raising greatly of the content of contained medicinal component chlorogenic acid, greatly promoted its economic benefit.
Claims (9)
1. a medicinal gold honeysuckle flower selection cross method, is characterized in that: comprise the following steps:
(1) Selection parent, take Shandong tetraploid honeysuckle as maternal, and Guizhou hairy honeysuckle is male parent;
(2) hybridization obtains kind, chooses a farmland as seed selection seedling field, by artificial pollination mode, obtains medicinal gold honeysuckle flower hybrid seed;
(3) sowing; The seeding quantity in every mu of seedling field is 0.600～0.800kg;
(4) transplant;
(5) disconnected branch;
(6) roguing, by observing, picks out that the relative parent of coring is long, short, the colored or large plant of the relative parent of bud of the relative parent of interval on branch;
(7) breed, set up strain;
(8) seed selection, the pest-resistant disease-resistant and drought resistance of the content of medicinal Chlorogenic Acid of Flos Lonicerae, the output of medicinal gold honeysuckle flower and plant of take is carried out seed selection to plant as standard;
(9) fixed kind, and breeding in batches.
2. selection cross method according to claim 1, is characterized in that: the hybrid seed obtaining in described step (2) is saved to sowing in next year at 0～5 ℃ of temperature.
3. selection cross method according to claim 1, it is characterized in that: the seedling field in described step (3) prior to seeding, every mu adds fertilizer 600～800kg, then kills virus with the liquor potassic permanganate that 0.25～0.35kg95% fenaminosulf soluble powder or 45～55g mass ratio are 0.5%.
4. selection cross method according to claim 1, is characterized in that: in described step (3), seed first passes through vernalization, then sows.
5. selection cross method according to claim 4, is characterized in that: the vernalization mode of seed is, first seed is placed in 25～35 ℃ of warm water and soaks 24 hours, then at room temperature puddles with wet sand.
6. selection cross method according to claim 1, is characterized in that: in described step (3), the seeding quantity in every mu of seedling field is 0.6300～0.6800kg.
7. selection cross method according to claim 1, is characterized in that: the seedling strain length of being transplanted in described step (4) is 15～20cm, and is balled transplanting.
8. selection cross method according to claim 1, is characterized in that: in described step (5), by the seedling strain length of disconnected branch, be 30～40cm.
9. selection cross method according to claim 1, is characterized in that: the mode of reproduction in described step (7) is that cuttage or press strip are bred.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108192830A (en) * | 2018-01-21 | 2018-06-22 | 广西大学 | A kind of bacterial strain S2-16 that can grow secretion secondary metabolites chlorogenic acid and its application |
| CN109601371A (en) * | 2019-01-02 | 2019-04-12 | 四川鑫沃康农业有限责任公司 | A kind of breed improvement method of honeysuckle |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108192830A (en) * | 2018-01-21 | 2018-06-22 | 广西大学 | A kind of bacterial strain S2-16 that can grow secretion secondary metabolites chlorogenic acid and its application |
| CN108192830B (en) * | 2018-01-21 | 2020-05-19 | 广西大学 | Strain S2-16 capable of growing and secreting secondary metabolite chlorogenic acid and application thereof |
| CN109601371A (en) * | 2019-01-02 | 2019-04-12 | 四川鑫沃康农业有限责任公司 | A kind of breed improvement method of honeysuckle |
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