CN105325282B - Oil Chinese herbaceous peony first cross kind producing method for seed and seed purposes - Google Patents
Oil Chinese herbaceous peony first cross kind producing method for seed and seed purposes Download PDFInfo
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- CN105325282B CN105325282B CN201510625973.9A CN201510625973A CN105325282B CN 105325282 B CN105325282 B CN 105325282B CN 201510625973 A CN201510625973 A CN 201510625973A CN 105325282 B CN105325282 B CN 105325282B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
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Abstract
The invention discloses oil Chinese herbaceous peony first cross kind producing method for seed and seed purposes, include the following steps：Selection is made maternal with high-quality, high yield, disease-resistant any or male sterile line of any two or three kinds of good characters from Bo Chinese herbaceous peony or river Chinese herbaceous peony seed bud；（2）Male parent is selected, selection is with high-quality, high yield, disease-resistant any or any two or three kinds of good characters and be that self-mating system under isolation situation makees male parent from class Chinese herbaceous peony seedling of setting seeds；（3）Select isolated area；（4）Transplant planting；（5）Florescence alternation；（6）Pollination；（7）Harvesting；A kind of seed purposes harvested from female parent from the producing method for seed：It is directly used in field seeding production；The seed purposes harvested from male parent：Produce edible oil raw material；It makes a living and gives birth to the male parent of generation seed.Good effect：Pioneering first cross kind；Production of hybrid seeds program is simplified, reduces production cost；Can meet the needs of large-scale production high-quality Chinese herbaceous peony and its seed and manufacture edible vegetable oil.
Description
Technical field
The invention belongs to field of plant breeding, and in particular to a kind of oil Chinese herbaceous peony first cross kind producing method for seed and seed
New application.
Background technology
Chinese herbaceous peony is hair section Paeonia perennial herb medicinal plant Chinese herbaceous peony, and root hyoscine, main component Chinese herbaceous peony is thermophilic, male
Red phenol etc., bitter are slightly cold, return liver warp, are that China's traditional Chinese medicine tradition commonly uses key medicine, 80 percent medicinal herb grower's plantation be all with
Farm variety based on Bo medicine, river medicine, and Bo medicine and river medicine will wait until that the growth of preceding season Chinese herbaceous peony harvests for many years mostly by vegetative propagation
Bud head separately as seedling is used afterwards, does not generate seed, breeding radix is low, and the period is long, is unfavorable for large area industrialization promotion
And mechanization production.
At present, the present inventor is made sexual numerous using the heterozygosis seed isolated after wild RADIX PAEONIAE RUBRA and family's kind paenoiae alba hybridization
It grows, the seed production is relatively low.According to professional institution research shows that：More than 20% crude fat contained by Chinese herbaceous peony seed contains in grease
Oleic acid, linoleic acid, leukotrienes ratio are between 25% -35%, the close equilibrium of three kinds of unrighted acid ratios, leukotrienes
Content be 50 times of homogenous quantities olive oil on the market, be current known best green health edible oil resource in the world it
One.
Modern nutriology is thought：Oleic acid in plant in unrighted acid, which has, adjusts high and low density in blood of human body
The effect of cholesterol concentration ratio；Linoleic acid has the function of to reduce cholesterol and blood fat in blood of human body；Leukotrienes has clear
It except human free radical strengthen immunity, reduction and stabilizing blood pressure and blood glucose, increases intelligence, protect eyesight and antioxidation, have
It high nutraceutical and develops and utilizes value, particularly in face of edible oils such as current genetically engineered soybean, cotton, corns to people
The possible threat that forms of health, the trend to go to the world along with the great rejuvenation of the Chinese nation and traditional Chinese medicine and become
Gesture, Chinese herbaceous peony seed edible oil have more irreplaceable strategic value and economic value.
The above-mentioned method for producing a small amount of Chinese herbaceous peony seed, referred to as conventional breeding method（Also it is systematic breeding method）, still, this system
There are still some problems for kind method, is mainly manifested in：Though the seed of plant harvest, low output, and these seeds belong to pole not
Stable allometrosis, offspring's separation is serious, and the production traits is extremely unstable, and kind property is poor, such as：Chinese herbaceous peony plant height is uneven, plants
Strain is universal thinner and more delicate, and extent of the root system, diameter are undesirable, and disease resistance is poor, and seed size is inconsistent, ripe also to differ sooner or later
It causes, silique（Seed）It is easily sagging, it is unfavorable for mechanized harvest and large area plantation, and this method will pass through selection and breeding, 4-7 generations
The programs such as selfing, the period is long, and program is cumbersome, manually pays of high cost.
Therefore, conventional breeding method（Also it is systematic breeding method）Because of production of hybrid seeds long flow path, seed properties are unstable, bring out seed again
Various drawbacks such as the Radix Paeoniae Alba quality that produces is not satisfactory seriously restrict the commercial development of Chinese herbaceous peony edible oil, also contribute to
The performance of some merits of Chinese medicine Chinese herbaceous peony.
Invention content
It is an object of the invention to provide a kind of oil Chinese herbaceous peony producing method for seed and seed purposes, this method is theoretical using three series mating
With technology, bear seeds, and the seed that maternal plant is born is tool on grain of being set seeds on paternal plant by this method, maternal plant
The Chinese herbaceous peony first cross kind of the excellent character of standby parents（F1）, field seeding production is can be directly used for, production of hybrid seeds flow is short, F1 generation character
Stablize, is good, and being produced down by the seed of the more conventional method of breeding production of next-generation grain yield of F1 generation sowing production
Generation seed is higher by 30-50%, and F1 generation Paeonia lactiflora seed meets the need of commercialization, large-scale production high-quality Chinese herbaceous peony and Chinese herbaceous peony seed
Will, Chinese herbaceous peony seed can meet the needs of manufacturing high-quality, high-grade vegetable edible oil.
To realize goal of the invention, the technical solution adopted is that：
1st, oil Chinese herbaceous peony first cross kind producing method for seed, it is characterised in that：Include the following steps：
（1）Selection is maternal
Selection is good with high-quality, high yield, disease-resistant any or any two or three kinds from Bo Chinese herbaceous peony or river Chinese herbaceous peony seed bud
The male sterile line of good character is made maternal；
（2）Select male parent
Selection is with high-quality, high yield, disease-resistant any or any two or three kinds of formedness from the seedling for class Chinese herbaceous peony of setting seeds
Shape and be that self-mating system under isolation situation makees male parent；
（3）Select isolated area
The regional arrangement hybrid seeding field of 5-10 kilometers of interior class Chinese herbaceous peony plantations of not blossoming and having seeds around, to exclude miscellaneous system
Interference；
（4）Transplant planting
To step（1）The maternal budding, male parent seedling, the maternal seed bud divided are transplanted to put in order by step
Suddenly（3）The big Tanaka of the isolated area is spaced field planting field in proportion；
（5）Florescence alternation promotes male parent, maternal florescence consistent；
（6）Pollination：It pollinates to female parent；
（7）Harvesting：When seed maturity, seed is harvested from paternal plant, seed is harvested from maternal plant（F1）.
Preferably to realize to maternal pollination, pollination is any in spontaneous pollination, supple-mentary pollination, artificial pollination or appoints
Two kinds of combination or three kinds of combination.
To promote male parent, the florescence of maternal plant consistent, step（5）In for have Blooming be inclined to male parent or mother
This, can orient foliage spray with the maleic hydrazide of 100ppm in bud stage and be bloomed with postponing it, sprinkling 1-3 times, per acre maleic hydrazide
The dosage of aqueous solution is 20-60 kilograms, promotes male parent, maternal florescence consistent.
To promote male parent, the florescence of maternal plant consistent, step（5）In for have postponement bloom tendency male parent or mother
This, can orient foliar spray to promote its Blooming in the gibberellic acid of bud stage 40ppm, spray 1-3 times, per acre gibberellic acid
Amount of aqueous solution used is 20-60 kilograms, promotes male parent, maternal florescence consistent.
For convenience of father, seed, seed later maintenance and the application of maternal harvest, including step（8）：The seed born to male parent
After grain, the maternal seed born separately harvest, dried, collected and managed and protected respectively.
The first purposes of product is directly obtained as the method for the present invention：It is a kind of to use oil Chinese herbaceous peony described in claim 1
The seed that medicine first cross kind producing method for seed is harvested from maternal plant, the seed are first cross kind（F1）, for crop field
Sowing production.
Second of purposes of product is directly obtained as the method for the present invention：It is a kind of to use oil Chinese herbaceous peony described in claim 1
The seed that medicine first cross kind producing method for seed is harvested from paternal plant, the purposes of the seed is as production edible vegetable oil
Raw material.
The third purposes of product is directly obtained as the method for the present invention：It is a kind of to use oil Chinese herbaceous peony described in claim 1
The seed that medicine first cross kind producing method for seed is harvested from paternal plant, the purposes of the seed is as claim 1 step（2）
The male parent of the next-generation seed of production.
The positive effect of the present invention is mainly manifested in：
1st, first cross kind is initiated.
Using this method, Chinese herbaceous peony first cross kind pioneering both at home and abroad is cultivated（F1）, first cross kind character stabilization, tool
Standby parents' advantageous feature（Show plant characteristic etc.）, the special seed of Production of Large Fields can be made, conventional breeding method is overcome and be made
Paeonia lactiflora seed for Production of Large Fields and obtain Chinese herbaceous peony low output, poor quality and seed it is unstable, it is easily separated, kind sexual involution problem.
2nd, production of hybrid seeds program is simplified, reduces seed production cost.
Conventional breeding method is first to hybridize the sexual method of breeding detached again, to pass through multiple selection and breeding, 4-7 generations are selfed final ability
The seed for meeting breeding objective is selected, program is cumbersome, and breeding time generally takes 5-10 years；And the method for the present invention is simple, is easy to slap
Hold, be easy to utilize, breeding time is short, can set seeds within general 2 years or so, high yield after 3-5, utilization periods up to 10 years with
On, reproductive efficiency is high, is easy to the large-scale production production of hybrid seeds and harvest.
3rd, the raw material needs for meet the industrialization of Chinese herbaceous peony oil, commercially producing.
Using Paeonia lactiflora seed produced by the invention and seed, it is suitble to large-scale plantation and breeds production again, and items contain
Oily nutritional ingredient index be not less than conventional breeding method seed, can meet industrialization, the raw material for commercially producing Chinese herbaceous peony oil need
It will.
4th, social and economic benefit is considerable.
Using this producing method for seed, the needs of Chinese herbaceous peony oil raw material in Chinese medicine, health products and food are on the one hand met, are people
The health and longevity of class contribute, and on the other hand, improve the mechanized cultivation, extensive of Chinese medicine Chinese herbaceous peony and Chinese herbaceous peony oil raw material
Plantation degree mitigates manual labor's burden, greatly improves the income of numerous farmers, therefore with good economic benefit and society
It can benefit.
Description of the drawings
Fig. 1 be male parent seedling of the present invention, maternal seed bud one of field ratio schematic diagram；
Fig. 2 be male parent seedling of the present invention, maternal seed bud field ratio schematic diagram two.
Specific embodiment
By following several embodiments, the present invention will be described in detail.
Embodiment 1
（1）Selection is maternal
Male sterile line of the selection with good character makees maternal seed bud from the Chinese herbaceous peony seed bud of river：Refer to chosen, choosing
Provide any one of high-quality, high yield, these three disease-resistant characteristics or any two combination or three kinds have entirely it is good character,
And the seed bud of the river Chinese herbaceous peony for male sterile line.The type of good character is how much, be depending on breeding objective, than if any
Breeding objective focuses on yield（Rhizome, kind grain yield）, when the male sterile line that focal selection has the good character of " high yield " is done
Female parent, breeding objective be focus on " high yield " and " disease-resistant " characteristic, when focal selection is provided simultaneously with " high yield " and " disease-resistant "
The male sterile line of two kinds of superperformances does female parent, and how breeding objective selects, and how good character type determines, belongs to existing
Technology, one skilled in the art can be achieved, and I will not elaborate.
The selection method of good character river Chinese herbaceous peony seed bud is conventional method, such as long-term observation, contrast test, analysis statistics
Deng, the selecting party prior art owned by France, one of ordinary skill in the art can be achieved, and I will not elaborate.
The embodiment of maternal seed bud is existing conventional agricultural methods, for example bud head is taken to breed：Cutting several piece is stayed
Bud buries sprout tuber soil pit cultivation etc., and I will not elaborate.
（2）Select male parent
From class Chinese herbaceous peony of setting seeds --- in wild RADIX PAEONIAE RUBRA seedling selection with good character and be to be selfed under isolation
System makees male parent seedling：It refers to chosen, there are high-quality, high yield, any one of these three disease-resistant characteristics or wantonly two in selecting
Kind of combination or three kinds of good characters having entirely and be self-mating system under isolation wild RADIX PAEONIAE RUBRA seedling.Formedness
The type of shape is how much, be depending on breeding objective, than if any breeding objective focus on yield（Rhizome, kind grain yield）, when
The self-mating system that focal selection has " high yield " good character makees male parent, breeding objective be the spy for focusing on " high yield " and " disease-resistant "
Property, when the self-mating system seedling that focal selection is provided simultaneously with " high yield " and " disease-resistant " two kinds of superperformances makees male parent, breeding objective is such as
What is selected, and how good character type determines, belongs to the prior art, and one skilled in the art can be achieved, here no longer
It is described in detail.
The selection method of the wild RADIX PAEONIAE RUBRA seedling of good character is conventional method, and such as long-term observation is measured, comparison, tried
It tests, analyze statistics etc., the selecting party prior art owned by France, one skilled in the art can be achieved, and I will not elaborate.
The wild RADIX PAEONIAE RUBRA self-mating system is usually that it is more than generation to be selfed 3, the selfing of preferably 3 generations, and selfing is real under isolation
Existing, isolation includes the partition methods such as space isolation, the isolation of artificial gauze, belongs to the prior art, one skilled in the art
It can be achieved, I will not elaborate.
The embodiment of male parent seedling is existing conventional agriculture method, such as conventional seed seedling-raising：It is broadcast including soil
Kind, germinating growth are Radix Paeoniae Alba seedling etc..I will not elaborate.
（3）Select isolated area
The regional arrangement hybrid seeding field of 5-10 kilometers of interior class Chinese herbaceous peony plantations of not blossoming and having seeds around, to exclude miscellaneous system
Interference.
Class of blossoming and having seeds Chinese herbaceous peony mainly includes wild class Chinese herbaceous peony of setting seeds, Hangzhoupro Chinese herbaceous peony, artificial culture " oily Chinese herbaceous peony No.1 ", wherein,
Wild class Chinese herbaceous peony of setting seeds mainly includes：Wild RADIX PAEONIAE RUBRA, mutation Peony, wild spends more Chinese herbaceous peony, wild single flower at root of Paeonia sinjiangensis
The kinds such as Chinese herbaceous peony.
（4）Transplant planting
In annual autumn（During August-November）, to step（1）The maternal budding by male parent seedling, has divided
Maternal seed bud be transplanted to put in order by step（3）The big Tanaka of the isolated area is spaced field planting field in proportion.
Signified " in proportion " interval field planting belongs to existing conventional techniques in the step, it is therefore an objective to convenient for pollinating and harvesting seed,
There is no fixed proportional manner, interval mode, one of ordinary skill in the art know, according to the range of pollination, uniformity coefficient and
Whether take artificial pollination and how to facilitate artificial pollination, how to facilitate harvest seed, voluntarily flexible selection percentage, interval mould
Formula.Such as：The present invention is preferably：As shown in Figure 1, ratio is at field margins：Left hand edge a line is maternal row, right hand edge phase
Adjacent male parent row：Maternal behavior 1:1, field middle section：The ratio of male parent row and maternal row is 1:2, also can as shown in Figure 2,
Field middle section：The ratio of male parent row and maternal row is 1:3 or other proportional spacings pattern, I will not elaborate.
（5）Florescence alternation promotes male parent, maternal florescence consistent.
For having the male parent of Blooming tendency, the maternal maleic hydrazide orientation foliage spray that 100ppm can be used in bud stage
It with late blooming, sprays 1-3 times, the dosage of maleic hydrazide aqueous solution is 20-60 kilograms per acre, promotes male parent, maternal florescence consistent；
For have postponement bloom tendency male parent, it is maternal can the gibberellic acid of bud stage 40ppm orient foliar spray with
Promote Blooming, spray 1-3 times, the dosage of gibberellic acid aqueous solution is 20-60 kilograms per acre, promotes male parent, maternal florescence one
It causes.
Maleic hydrazide, gibberellic acid specifically spray number and per acre dosage, more according to the time that male parent, maternal bud stage are staggered
It is few, voluntarily flexibly grasp.
（6）Pollination：It pollinates to female parent.
Preferably to realize to maternal pollination, pollination is any in spontaneous pollination, supple-mentary pollination, artificial pollination or appoints
Two kinds of combination or three kinds of combination.
Supple-mentary pollination mainly puts bee in a suitable place to breed to realize using wild bee or manually.
（7）Harvesting：When seed maturity, seed is harvested from paternal plant harvesting seed, maternal plant（F1）.
The seed for harvesting seed on paternal plant, bearing on maternal plant is separately harvested during harvest, applied to different
Purposes, the seed that the seed that can also harvest paternal plant, maternal plant harvest mix, and it is edible to be completely used for manufacture
Oil.
（8）Divide and shine, point Tibetan, be in charge of：The seed of the seed of male parent harvest, maternal harvest is dried respectively during harvest, is collected
With manage and protect, as the later stage produce and apply.
Also can selectively it be included according to situation on the spot in the above process：Site preparation basal dressing, root outer spraying, pest and disease damage are prevented
Control, tillage and weeding, field management, belong to conventional prior, I will not elaborate.
Embodiment 2
（1）Selection is maternal
Male sterile line of the selection with good character makees maternal seed bud from Bo Chinese herbaceous peony seed bud：Refer to chosen, choosing
Provide any one of high-quality, high yield, these three disease-resistant characteristics or any two combination or three kinds have entirely it is good character,
And it is the Bo Chinese herbaceous peony seed bud of male sterile line.How good character type determines, the seed bud selection method of good character Bo Chinese herbaceous peony
With 1 step of embodiment（1）In " river Chinese herbaceous peony " it is identical, one skilled in the art can be achieved, I will not elaborate.
The embodiment of Bo Chinese herbaceous peony seed bud is existing conventional agricultural methods, with 1 step of embodiment（1）In " river Chinese herbaceous peony " plant
The embodiment of bud is identical, and I will not elaborate.
（2）Select male parent
From class Chinese herbaceous peony of setting seeds --- in the Chinese herbaceous peony seedling of Hangzhoupro selection with good character and be self-mating system under isolation
Make male parent seedling：It refers to chosen, selects with any one of high-quality, high yield, these three disease-resistant characteristics or any two groups
Close or three kinds of have entirely characters and be self-mating system under isolation Hangzhoupro Chinese herbaceous peony seedling.How is good character type
It determines, Hangzhoupro Chinese herbaceous peony seedling selection method and 1 step of embodiment of good character（2）In " wild RADIX PAEONIAE RUBRA " it is identical, affiliated neck
Domain those of ordinary skill can be achieved, and I will not elaborate.
1 step of Hangzhoupro Chinese herbaceous peony self-mating system, isolated instances and embodiment（2）In " wild RADIX PAEONIAE RUBRA " it is identical, fields are general
Logical technical staff can be achieved, and I will not elaborate.
The embodiment of Hangzhoupro Chinese herbaceous peony seedling is existing conventional agriculture method, with 1 step of embodiment（2）In " wild RADIX PAEONIAE RUBRA "
Type of rearing is identical, and I will not elaborate.
Step（3）—（8）With step in embodiment 1（3）—（8）Correspondence is identical, therefore omits.
Embodiment 3
First, a kind of novel class Chinese herbaceous peony of setting seeds is introduced --- " oily Chinese herbaceous peony No.1 ", " oily Chinese herbaceous peony No.1 "（Code name, similarly hereinafter）It is one
Kind Chinese herbaceous peony new varieties are that the present inventor utilizes the famous Radix Paeoniae Alba variety source Hangzhoupro Chinese herbaceous peony farm variety in China and wild herbaceous peony to carry out
Mostly for selection cross（I.e. aforementioned conventional breeding method）It forms, which generates a small amount of seed, grain 30 of averagely setting seeds per fruit
Left and right, the kind plant is 1-1.5 meters high, and blade is more roomy, and blade face is relatively thin, and color is shallower, and seed oil content, should more than 20%
Kind medicinal effects（Rhizome）Up to 1500 kilograms or more, I and II ratio defective product is high for the general per mu yield of life in 4 years, increases than farm variety
Production 15% -20%（In the case where not influencing seed production）, it is easily sagging after seed is full, it is unfavorable for mechanized harvest, Paeoniflorin
Content is higher by more than 20% than farm variety.
（1）Selection is maternal
Step in the present embodiment（1）" selection and breeding are maternal " and the step in embodiment 1（1）" selection and breeding are maternal " content is identical, therefore
It omits.Female parent selection object is " river Chinese herbaceous peony " or " Bo Chinese herbaceous peony ".
（2）Select male parent
From class Chinese herbaceous peony of setting seeds --- in " oily Chinese herbaceous peony No.1 " seedling selection with good character and be under isolation from
System is handed over to make male parent seedling：It refers to chosen, selects with any one of high-quality, high yield, these three disease-resistant characteristics or wantonly two
Kind combination or three kinds of good characters having entirely and be self-mating system under isolation " oily Chinese herbaceous peony No.1 " seedling, good character
Type how to determine, " oily Chinese herbaceous peony No.1 " the seedling selection method of good character and step in embodiment 1（2）" the wild radix paeoniae rubrathe
Medicine " is identical, and one skilled in the art can be achieved, and I will not elaborate.
" oily Chinese herbaceous peony No.1 " 1 step of self-mating system, isolated instances and embodiment（2）In " wild RADIX PAEONIAE RUBRA " it is identical, affiliated neck
Domain those of ordinary skill can be achieved, and I will not elaborate.
" oily Chinese herbaceous peony No.1 " seedling fostering mode is existing conventional agriculture method, with 1 step of embodiment（2）In " the wild radix paeoniae rubrathe
Medicine " type of rearing is identical, and I will not elaborate.
The present embodiment step（3）—（8）With step in embodiment 1（3）—（8）Correspondence is identical, therefore omits.
Embodiment 4
Step in the present embodiment（1）" selection is maternal " and the step in embodiment 1（1）" selection is maternal " content is identical.Therefore
It omits.
（2）Select male parent
From class Chinese herbaceous peony of setting seeds --- self-mating system of the selection with good character makees male parent kind in " mutation Peony " seedling
Seedling：Refer to it is chosen, select with any one of high-quality, high yield, these three disease-resistant characteristics or any two combination or three kinds
The character that has entirely and be self-mating system under isolation mutation Peony seedling, how good character type to determine,
" mutation Peony " the seedling selection method of good character and 1 step of embodiment（2）In " wild RADIX PAEONIAE RUBRA " it is identical, it is affiliated
Field those of ordinary skill can be achieved, and I will not elaborate.
" mutation Peony " self-mating system, 1 step of isolated instances and embodiment（2）In " wild RADIX PAEONIAE RUBRA " it is identical, institute
Category field those of ordinary skill can be achieved, and I will not elaborate.
" mutation Peony " seedling embodiment is existing conventional agriculture method, with step in embodiment 1（2）In it is " wild
Raw RADIX PAEONIAE RUBRA " cultivation embodiment is identical, and I will not elaborate.
The present embodiment step（3）—（8）With step in embodiment 1（3）—（8）Correspondence is identical, therefore omits.
Embodiment 5
Step in the present embodiment（1）" selection is maternal " and the step in embodiment 1（1）" selection is maternal " content is identical.Therefore
It omits.
（2）Select male parent
From class Chinese herbaceous peony of setting seeds --- self-mating system of the selection with good character makees male parent seedling in " root of Paeonia sinjiangensis "：Refer to
It is chosen, select what is had entirely with any one of high-quality, high yield, these three disease-resistant strong characteristics or any two combinations or three kinds
Character and be self-mating system under isolation root of Paeonia sinjiangensis seedling, how good character type to determine, good character
The selection method of " root of Paeonia sinjiangensis " seedling and 1 step of embodiment（2）" wild RADIX PAEONIAE RUBRA " it is identical, fields ordinary skill people
Member can be achieved, and I will not elaborate.
1 step of " root of Paeonia sinjiangensis " self-mating system, isolated instances and embodiment（2）In " wild RADIX PAEONIAE RUBRA " it is identical, affiliated neck
Domain those of ordinary skill can be achieved, and I will not elaborate.
" root of Paeonia sinjiangensis " seedling embodiment is existing conventional agriculture method, with step in embodiment 1（2）In it is " wild red
Chinese herbaceous peony " type of rearing is identical, and I will not elaborate.
The present embodiment step（3）—（8）With step in embodiment 1（3）—（8）Correspondence is identical, therefore omits.
In addition, the method for the present invention step can be used as（2）Described in the Chinese herbaceous peony of male parent seedling further include:It is wild spend more Chinese herbaceous peony,
The red spoon of mutation hair, beautiful Chinese herbaceous peony, careless Chinese herbaceous peony and its mutation Mao Yecao Chinese herbaceous peonies, white flower Chinese herbaceous peony, Paeonia anomala etc. are set seeds class Chinese herbaceous peony.
Apart from the above, the father by the use of the method for the present invention when the seed harvested in male parent can be as production next generation's seed
This, can refer to step（2）, to select to use.
Below by some experiments, detection, to illustrate the positive effect of the present invention.
Experiment one：
Inventor has many advantages through many experiments, using the present invention than conventional breeding method, such as：In October, 2011
Plantation was checked and accepted on the 10th in August in 2014, and the present inventor is in farmland covered by contract plantation " the miscellaneous No.1 of oil of Sha Ji townshiies of Yucheng County of Henan Province 1
“（The Hybrid F1 is named as " oily miscellaneous by the first cross kind F1 obtained using producing method for seed of the present invention in female parent, the present inventor
No.1 ", similarly hereinafter）It one mu, plants " oily Chinese herbaceous peony No.1 "（Male parent）It one mu, is compared through analysis, conclusion such as following table：
| The production of hybrid seeds period | Whether mechanization | Production of hybrid seeds step | Seed appearance | Whether production seed includes First cross kind | Grain yield（2014 Year）（Pound/acre） | |
| The present invention Method | Seed is harvested after 2 years, every year Harvest is primary. | Cluster in silique, is highly convenient for mechanization Harvest. | Simply, it is easy to operation | It is full, in the same size | It is（It is harvested on female parent） | 235 |
| Routine is educated Kind method | Seed is harvested after 3 years, every year Harvest is primary. | It is big-eared under silique, it is impossible to mechanical harvest, It can only manually harvest. | It is cumbersome, it be through excessive generation point From unfavorable operation | Having flat has full, size It is inconsistent | It is no | 158 |
Experiment two：
August obtains result of the test on 1st within 2014：Inventor congratulates certain farmland covered by contract in Sha Ji townshiies of Yucheng County of Henan Province Sha Ji West Streets
Six mu, wherein three mu of plantation " the miscellaneous No.1 of oil ", life in 3 years " the miscellaneous No.1 of oil " 310 jin of average yield per mu seed, are planted " oily Chinese herbaceous peony No.1 "
Three mu, life in 3 years " oily Chinese herbaceous peony No.1 " 220 jin of average yield per mu seed, amount of increase in production is up to 40.9%.
Experiment three:
On July 30th, 2015 obtains result of the test：Inventor is in Sha Ji townshiies of Yucheng County of Henan Province Ren Qiaocunhuang buildings villager's group
Conventional kind one mu of the oily Chinese herbaceous peony No.1 of Ren's farmland covered by contract, one mu of plantation " the miscellaneous No.1 of oil ", 4 years raw 480 jin of per mu yield seeds, plantation " ", together
290 jin of per mu yield seed after sample 4 years, amount of increase in production is up to 65.5%.
Experiment four：
On July 26th, 2015, inventor is in Sha Ji townshiies of Yucheng County of Henan Province Ren Qiao villages Lee's farmland covered by contract, two mu of Radix Paeoniae Albas of plantation
（It is life in 4 years）, one mu of Radix Paeoniae Alba（Code name：Chinese herbaceous peony A）It is the first cross kind obtained by the present invention（F1）(code name：Oil miscellaneous one
Number) generation first generation Radix Paeoniae Alba, one mu of Radix Paeoniae Alba（Code name：Chinese herbaceous peony B）It is that seed is obtained by conventional breeding method（Code name：Oily Chinese herbaceous peony one
Number）The Radix Paeoniae Alba of generation, through measuring, detecting, results of comparison see the table below plant characteristic between the two：
Detection 1：
The first cross kind obtained by producing method for seed of the present invention（F1）（Code name：The miscellaneous No.1 of oil）Grow up to the Peony Root of harvest
Stem, the present inventor deliver Bozhou City Chinese medicine inlet and outlet inspection center of Anhui Province, after testing, detection knot on March 27th, 2014
Fruit is as follows：
《Chinese Pharmacopoeia》Regulation：Paeoniflorin content is not less than 1.8% in Radix Paeoniae Alba.By the Chinese herbaceous peony of F1 generation of the present invention production Chinese herbaceous peony
Glycosides content is 3.3%, is far higher by the regulation of pharmacopeia, " Radix Paeoniae Alba B " paeoniflorin content 2.4% being also apparently higher than in " experiment four ".
Above experiment, testing result fully show that the present invention more conventional method of breeding time is short, and production of hybrid seeds flow is few, breeding effect
Rate is high, it is most important that this method produces the Chinese herbaceous peony first cross kind for having the excellent character of parents（F1）（Inventor names certainly
Its code name：The miscellaneous No.1 of oil）, field seeding production is can be directly used for, F1 generation character is stable, good, and F1 generation sowing production
The next-generation more conventional method of breeding of grain yield is higher by 30-50%, and the quality of Radix Paeoniae Alba formed, yield, pharmacological property etc. are bred by F1 generation
Index is substantially better than the Radix Paeoniae Alba that conventional breeding method brings out seed production.
Detection 2：
The Radix Paeoniae Alba seed harvested in male parent by the method for the present invention（Dry product）, inventor is on October 15th, 2014 to agriculture
Ye Bu agricultural product qualities supervision and inspection center（Zhengzhou）It proposes aliphatic acid inspection request, is examined through the center, survey report
Middle Radix Paeoniae Alba seed（Dry product）Every aliphatic acid index such as following table：
By this report it can be seen that its seed can be used as good edible oil raw material.Oleic acid, linoleic acid, linolenic finger
Mark is higher, and ratio is more balanced, full of nutrition, adapts to very much the raw material as pure natural health food oil.
Detection 3：
The Radix Paeoniae Alba grain seed harvested in male parent by the method for the present invention（Dry product）The vegetable oil squeezed, inventor was in 2014
October 15 is to agricultural product quality supervision and inspection center of the Ministry of Agriculture（Zhengzhou）It proposes aliphatic acid inspection request, is examined through the center
It tests, Radix Paeoniae Alba seed in survey report（Dry product）Every aliphatic acid index such as following table：
By this report it can be seen that the Radix Paeoniae Alba oil oleic acid, linoleic acid, linolenic index are higher and very balanced, very
Close to 1:1:1, without other poisonous and harmful elements, some existing oil plant type seed (such as peanut, soybean, camellia oilseeds etc.) institutes
Oleic acid, linoleic acid, the leukotrienes of the oil of squeezing reach or not yet close to above-mentioned scientific and rational natural ratios 1:1:1, therefore,
The raw material of pure natural, healthy non-polluted edible oil is highly suitable as by Chinese herbaceous peony seed produced by the invention, can meet commercialization,
Extensive oil refining needs.
The figure of above-mentioned expression, explanation, only several preferred embodiments of the invention are not the guarantors to the present invention
Shield range is limited, to those skilled in the art, under the premise of spirit and scope of the present invention is not departed from, this hair
Bright to be likely to change and improve, these changes and improvements should all fall into claimed invention right
It is interior.
Claims (5)
1. oil Chinese herbaceous peony first cross kind producing method for seed, which is characterized in that include the following steps：
（1）Selection is maternal
Male sterile line of the selection with high-quality, high yield, disease-resistant three kinds of good characters is made maternal from Bo Chinese herbaceous peony seed bud；
（2）Select male parent
Selection is with high-quality, high yield, disease-resistant three kinds of good characters in artificial culture Chinese herbaceous peony seedling from code name for " oily Chinese herbaceous peony No.1 "
And be that self-mating system makees male parent under isolation situation；" the oily Chinese herbaceous peony No.1 " is to make parent by Hangzhoupro Chinese herbaceous peony and wild herbaceous peony, through first miscellaneous
Friendship detaches again, the class Chinese herbaceous peony of setting seeds that 4-7 is formed for selfed breeding；" the oily Chinese herbaceous peony No.1 " grain oil content is more than 20%；
（3）Select isolated area
The regional arrangement hybrid seeding field of 5-10 kilometers of interior class Chinese herbaceous peony plantations of not blossoming and having seeds around, it is dry to exclude miscellaneous system
It disturbs；
（4）Transplant planting
To step（1）Male parent seedling, the maternal seed bud divided are transplanted to the step of having put in order by the maternal budding（3）
The big Tanaka of the isolated area is spaced field planting field in proportion；
（5）Florescence alternation promotes male parent, maternal florescence consistent；
（6）Pollination：It pollinates to female parent；
（7）Harvesting：When seed maturity, seed is harvested from paternal plant, seed is harvested from maternal plant（F1）.
2. oil Chinese herbaceous peony first cross kind producing method for seed according to claim 1, it is characterised in that：Step（6）Pollination is
Any or wantonly two kinds of combination or three kinds of combination in spontaneous pollination, supple-mentary pollination, artificial pollination.
3. oil Chinese herbaceous peony first cross kind producing method for seed according to claim 1, it is characterised in that：Step（5）In for
There are the male parent or female parent that Blooming is inclined to, bloomed in bud stage with the maleic hydrazide orientation foliage spray of 100ppm with postponing it,
Sprinkling 1-3 times, the dosage of maleic hydrazide aqueous solution is 20-60 kilograms per acre, promotes male parent, maternal florescence consistent.
4. oil Chinese herbaceous peony first cross kind producing method for seed according to claim 1, it is characterised in that：Step（5）In for
There is postponement to bloom the male parent or female parent of tendency, in bud stage with the gibberellic acid orientation foliar spray of 40ppm it to be promoted to open in advance
Flower sprays 1-3 times, and gibberellic acid amount of aqueous solution used is 20-60 kilograms per acre, promotes male parent, maternal florescence consistent.
5. oil Chinese herbaceous peony first cross kind producing method for seed according to claim 1, it is characterised in that：Including step（8）：It is right
After seed that male parent is born, the maternal seed born separately harvest, dried, collected and managed and protected respectively.
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| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
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