CN109601372A - A kind of breed improvement method of Radix Paeoniae Alba - Google Patents
A kind of breed improvement method of Radix Paeoniae Alba Download PDFInfo
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- CN109601372A CN109601372A CN201910002394.7A CN201910002394A CN109601372A CN 109601372 A CN109601372 A CN 109601372A CN 201910002394 A CN201910002394 A CN 201910002394A CN 109601372 A CN109601372 A CN 109601372A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a kind of breed improvement methods of Radix Paeoniae Alba in white peony improving technology field, and specific step is as follows for the modification method: S1: single-lobe Radix Paeoniae Alba and more valve Radix Paeoniae Albas being selected to carry out the mixing breed of artificial pollination；S2: with adopting with broadcasting after seed is mature, line-spacing 5cm water furrow planting is pressed on furrow, earthing 1cm sprays water 1 time for every 2 days, and more than 30 days can emerge, and after autumn or the 2nd year spring transplanted；S3: finally carrying out ramificatiom breeding to plant bred seedling, and the genuine certified products after improvement of the white peony in the present invention, active constituent content is high, and yield increases, and cost-effectively is constant, and resistance is stronger, wide adaptability.
Description
Technical field
The invention discloses a kind of breed improvement methods of Radix Paeoniae Alba, specially white peony improving technology field.
Background technique
Radix Paeoniae Alba, Chinese medicine name.For the dry root of ranunculaceae plant Chinese herbaceous peony.It summer, the excavation of two season of autumn, cleans, removes end to end and carefully
Root is set and is boiled again after removing crust or peeling after boiling in boiling water, dries.With nourishing blood for regulating menstruation, astringing YIN to stop sweating, soft liver analgesic is stabilized
The effect of liver-yang.It is usually used in blood deficiency chlorosis, irregular menstruation, spontaneous perspiration, night sweat, hypochondriac pain, abdominal pain, four limbs contraction pain, headache and dizziness.Tradition
White peony limited due to being planted the conditions such as weather, temperature in the place of production by it, medical value difference is very big, much too late
The pharmacological property of genunie medicinal materials.For this purpose, a kind of breed improvement method we have proposed Radix Paeoniae Alba comes into operation, to solve above-mentioned ask
Topic.
Summary of the invention
It is mentioned above in the background art to solve the purpose of the present invention is to provide a kind of breed improvement method of Radix Paeoniae Alba
Problem.
To achieve the above object, the invention provides the following technical scheme: a kind of breed improvement method of Radix Paeoniae Alba, the improvement side
Specific step is as follows for method:
S1: single-lobe Radix Paeoniae Alba and more valve Radix Paeoniae Albas is selected to carry out the mixing breed of artificial pollination；
S2: with adopting with broadcasting after seed is mature, pressing line-spacing 5cm water furrow planting on furrow, and earthing 1cm sprays water 1 time for every 2 days, and 30
Yu can emerge, and after autumn or the 2nd year spring transplanted；
S3: ramificatiom breeding finally is carried out to plant bred seedling.
Preferably, in the step S1, specific step is as follows for artificial pollination:
S11: using single-lobe Radix Paeoniae Alba as male parent, more valve Radix Paeoniae Albas are as maternal, collection paternal pollen, by the paternal pollen of collection
It is dipped on maternal gynoecium with writing brush, it is primary every 2~3d pollination, continuously pollinate 3~4 times；
S12: fresh idea is cut after 40~60d seed is mature, acquires seed after drying.
Preferably, in the step S11, flower of female parent crown scissors should be gone to big portion in artificial pollination, retains 1~5cm of base portion,
Maternal pistil stigma, and the bagging after each pollination can not be injured, extracts bag after last 1 pollination 1 week.
Preferably, in the step S11, optimal pollination time is fine day 10~12 point in one day, and in pollination 15
Extra flower is wiped out when fresh idea is aging after~20d, to reduce nutrient consumption, increases sunlight and penetrates, be conducive to seed development at
It is ripe.
Compared with prior art, the beneficial effects of the present invention are: white peony in the present invention genuine certified products after improvement,
Active constituent content is high, and yield increases, and cost-effectively is constant, and resistance is stronger, wide adaptability.
Detailed description of the invention
Fig. 1 is present invention hybridization flow chart.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, the present invention provides a kind of technical solution: a kind of breed improvement method of Radix Paeoniae Alba, the modification method
Specific step is as follows:
S1: single-lobe Radix Paeoniae Alba and more valve Radix Paeoniae Albas is selected to carry out the mixing breed of artificial pollination, the specific steps of artificial pollination are such as
Under:
S11: using single-lobe Radix Paeoniae Alba as male parent, more valve Radix Paeoniae Albas are as maternal, collection paternal pollen, by the paternal pollen of collection
It is dipped on maternal gynoecium with writing brush, it is primary every 2~3d pollination, continuously pollinate 3~4 times, it should be by flower of female parent in artificial pollination
Crown scissors go to big portion, retain 1~5cm of base portion, can not injure maternal pistil stigma, and the bagging after each pollination, last 1 pollination
Bag is extractd after 1 week, optimal pollination time is fine day 10~12 point in one day, and when fresh idea is old after the 15~20d that pollinates
Extra flower is wiped out when ripe, to reduce nutrient consumption, increases sunlight and penetrates, it is mature to be conducive to seed development；
S12: fresh idea is cut after 40~60d seed is mature, acquires seed after drying；
S2: with adopting with broadcasting after seed is mature, pressing line-spacing 5cm water furrow planting on furrow, and earthing 1cm sprays water 1 time for every 2 days, and 30
Yu can emerge, and after autumn or the 2nd year spring transplanted；
S3: ramificatiom breeding finally is carried out to plant bred seedling.
In the planting process of white peony, period in early spring loosens the soil soil, and weeding is according to circumstances carried out after emergence, and
Appropriate to implement to earth up to root, 10 lis or so from the ground of branches and leaves are extractd before freezing in period early winter, ground, carry out again
Ridging is conducive to white peony in this way and spends cold winter.Second year starts, and applies respectively in March and April to it
Fertilizer, it is best with night soil.Release amount is selected according to the actual situation per acre, between waiting until during November, can carry out barnyard manure, often
Mu increases dose.It is applied fertilizer twice before arriving soon harvest later.Extra bud is extractd every year, it is appropriate to be filled
It irrigates.
The prevention and control of plant diseases, pest control of white peony:
Radix Paeoniae Alba disease is more, common are gray mold and rust, can remove killed branches and leaves and burn；It to be arranged in time after rain
Water keeps field ventilation and penetrating light；Early stage sprays 1:1；100 Bordeaux mixture, 1 even spray 3 time of spray in 10 days.Rust, it is first in morbidity
Phase is prevented and treated by spraying with 97% sulfonat, 400 times of liquid.Insect pest has grub to bite rhizome, and bait trapping can be mixed with 90% metrifonate；
Radix Paeoniae Alba leaf mold is mainly caused harm Radix Paeoniae Alba blade.Nascent circle brown scab, circle of coming into being puce scab, size
6-15mm, rear micro- light brown wheel line of tool, the dark violet brown of surrounding, blade back often give birth to the mould layer of blackish green suede, and the fusion of later period scab causes leaf
It is withered.Stem, which is caught an illness, grows bar shaped puce scab.Petiole petiole base crotch of catching an illness easily is split off.Sepal, petal are caught an illness nascent purple
Dots in red, back edge are shrivelled.Its route of transmission and onset condition are overwintering on invalid body with mycelia and conidium, next year 3
The middle of the month, the last ten-days period, when condition is suitable for, the conidium of generation first encroaches on the lower blade of host, after gradually extend up, 7-Augusts
Heavier, the control measure specifically used occurs for the rainy season disease are as follows: ventilation and penetrating light is wanted in rational close planting between strain；Increasing application phosphorus potassium fertilizer, enhancing
Plant premunition；Early stage sprays 250 times of liquid of 50% carbendazim, 800~1000 times of liquid or 40% aliette.
White peony pharmacological action:
Central inhibitory action: Radix Paeoniae Alba has obvious analgesic activity, and Chinese herbaceous peony water decoction 0.4g (crude drug)/10g stomach-filling can significantly press down
Mouse acetic acid twisting reaction processed.5~40mg/kg of white peony root's total glycoside, intramuscular or intraperitoneal injection are turned round in dose-dependently inhibition mouse
Body is shouted and is reacted with hot plate, and inhibits rat hotplate reaction in 50~125mg/kg intraperitoneal injection.The ED50 of mouse writhing method
For 27mg/kg, the ED50 of hot plate method is 21mg/kg.Act on the 0.5~l hour of peak upon administration.Furthermore still reinforce respectively
Morphine, clonidine inhibit the effect of mouse writhing reaction.The analgesic activity of general glycoside may have high-level center participation, but not receiving Lip river
The influence of ketone.Radix Paeoniae Alba has sedation, and 1g/kg intraperitoneal injection can inhibit spontaneous activity in mice, enhance urging for ciclobarbital soluble
The hypnosis time that can also extend yellow Jackets is subcutaneously injected in dormancy effect, Chinese herbaceous peony injection.Paeoniflorin 1mg/ only, is infused in the ventricles of the brain
Enter, keep rat calm, 5~10mg causes sleep and of flaccid muscles.Paeoniflorin is applied alone sedation weaker, with licorice ingredient
FM100 has shared synergistic effect.Radix Paeoniae Alba has weaker anti-pentylenetetrazol convulsion effect, and Chinese herbaceous peony medicinal extract can fight strychinine-induced convulsion.Chinese herbaceous peony
Medicine glucoside has weaker cooling and refrigeration function to mouse normal body temperature and artificial fever animal；
Spasmolysis: Chinese herbaceous peony or Paeoniflorin have inhibition or spasmolysis to smooth muscle, can inhibit guinea pig in vitro small intestine oneself
Hair property is shunk, and makes the reduction of its tension, and can fight the contraction of cavy caused by barium chloride and Isolated rabbit intestine, to acetylcholine institute
It causes rat small intestine in vitro contraction to have no significant effect, but adds with significantly inhibiting effect after Radix Glycyrrhizae.The water decoction-alcohol sedimentation liquid 2g (crude drug) of Radix Paeoniae Alba/
Kg intravenous injection has obvious inhibiting effect to stomach and intestine bioelectricity, reduces the stomach electricity of anesthetized cat and intestines electricity slow wave amplitude, the period prolongs
It is long.Smooth muscle spasmolysis mechanism of action may be direct effect or parasympathetic nerve tip inhibited to discharge acetylcholine.Also it has been reported that
Radix Paeoniae Alba decoction increases the amplitude of isolated rabbit intestines Spontaneous Contraction, and has dosage correlation.In addition, Chinese herbaceous peony or Paeoniflorin are to branch gas
Pipe and uterine smooth muscle also have certain inhibiting effect, and can fight uterine contraction caused by oxytocins.Peony extract to mouse from
Body uterus low concentration is excited, and high concentration inhibits；
Anti-inflammatory, anti-ulcer effect: Chinese herbaceous peony or Paeoniflorin have weaker anti-inflammatory effect, to yeast, Irish moss colloidality and the right side
Revolving sugared acid anhydride pedal swelling has different degrees of inhibiting effect, synergistic effect has been shared with licorice ingredient FM100, to abdominal cavity blood capillary
Pipe permeability also has weaker inhibiting effect.Radix paeoniae alba extraction has inhibition to make rat egg white acute inflammation and granuloma induced by implantation of cotton pellets
With.White peony root's total glycoside 50mg/kg continuous 11 days, has obvious inhibiting effect to Rat Experimental adjuvant-induced arthritis one time a day.Chinese herbaceous peony
Contained paeonol, benzoylpaeoniflorin and oxidized paeoniflorin glycosides also have anti-inflammatory effect in medicine.Paeoniflorin has rat stress ulcer
Prevention effect, share in pylorus-ligated rats and FM100 has synergistic effect in terms of inhibiting gastric secretion, but Chinese herbaceous peony extracting solution makes
Gastric acidity slightly rises.
Influence to body's immunity: Radix Paeoniae Alba can promote the phagocytic function of macrophage in vivo and in vitro.Radix Paeoniae Alba
0.4g/ stomach-filling of decoction continuous 5 days, there is the phagocytic percentage of Turnover of Mouse Peritoneal Macrophages and phagocytic index one time a day
It significantly improves.1.2g/ only, one time a day, continuous 8 days, can make Immunosuppressant Cyclophosphamide induced mice peripheral blood acid.α-
The reduction of acetic acid esterase (ANAE) positive lymphocyte restores normal, and dramatically increases Hemolysin formation.Experiment shows white
Chinese herbaceous peony has humidification to cellular immunity and humoral immunity.White peony root's total glycoside (TGP) has rat assist agent arthritis (AA) anti-inflammatory
With function dependent immunity adjustment effect, 50mg/kg stomach-filling continuous 11 days, while obviously inhibition to AA, makes AA one time a day
The raised hydrogen peroxide of rat peritoneal macrophages (H2O2) and interleukin-11 (IL-1) are horizontal reduces, and keeps AA rat low
The ability that the reaction of thymocyte mitogen and splenic lymphocytes generate IL-2 restores normal.0.09~11.25 μ of white peony root's total glycoside
G/ml discharges H2O2 to the peritoneal macrophage that zymosan induces, and 0.5~12.5 μ g/ml synthesizes lipopolysaccharide-induced IL-1
IL-2 is generated to the rat spleen cells that con A (Con A) induces with 0.5~62.5 μ g/ml, has concentration dependent double
To adjustment effect, low concentration enhancing, high concentration inhibits, and amount effect curve is in " ∧ " shape.White peony root's total glycoside 200mg/kg stomach-filling, daily 1
It is secondary, continuous 8 days, there is humidification to mouse delayed allergy (DTH), 5mg/kg is injected intraperitoneally, one time a day, continuous 5~
8, caused by cyclophosphamide DTH is enhanced and is inhibited and the reduction of Hemolysin formation amount has significant antagonism, but is filled in over the ground
The inhibition of DTH caused by meter Song has no significant effect.Show that white peony root's total glycoside is to immune function with the experiment of monoclonal antibody indirect immunofluorescence
The mechanism of energy dual regulation is related with the ratio of helper T lymphocyte (TH) suppressor T lymphocyte (Ts) is adjusted；
Influence and resisting oxygen lack to cardiovascular system: Radix Paeoniae Alba and Paeoniflorin have expansion blood vessel, increase organic blood flow volume
Effect.Chinese herbaceous peony decoction can expand toad internal organ and isolated rabbit ear blood vessel.Radix Paeoniae Alba injection 2g (crude drug)/kg intravenous injection is immediately
Anesthetized cat visceral blood flow is increased considerably, and cardiomotility is slightly reinforced.Paeoniflorin can expand dog coronary vasodilator and limb
Body blood vessel has dosage correlation hypotensive activity to cavy.White peony root's total glycoside can significantly improve the hypoxia-bearing capability of animal, 5~
40mg/kg intraperitoneal injection, can dose-dependently extend the mouse normobaric hypoxia time-to-live, 20mg/kg can extend hypobaric hypoxia
Time-to-live；2.5~5mg/kg intracerebroventricular injection can be obviously prolonged the normobaric hypoxia time-to-live, show related with maincenter；40mg/
Kg can be reduced the death rate of mouse potassium cyanide poisoning anoxic, show directly improve cellular respiration；H1 receptor antagonist chlorobenzene
The resisting oxygen lack of that quick significant antagonism intracerebroventricular injection white peony root's total glycoside of energy, shows receptor related with H1；Furthermore resisting oxygen lack
It may also be related with the cooling effect of Radix Paeoniae Alba；
Influence to hematological system: peony extract 5mg/kg and 25mg/kg intraperitoneal injection make rat blood serum urea nitrogen
(BUN) it significantly reduces, 1,2,3,4,6- five galloyl glucose of effective component 1mg/, 2.5mg/ or 5mg/ is just
There is remarkable effect.Radix paeoniae alba extraction lectin (agglutinins) can improve rabbit anaemia caused by acute bleeding, prednisone acetate
Dragon can antagonism this effect.Paeoniflorin in vitro or intravenous injection, to ADP induction rat platelet aggregation have inhibiting effect, benzene
Formyl Paeoniflorin also plays the role of inhibiting platelet aggregation；
Antibacterial action: the antibacterial action of Radix Paeoniae Alba is stronger, and antimicrobial spectrum is wider.In vitro to staphylococcus aureus, haemolysis
It is property streptococcus, Streptococcus viridans, streptococcus pneumonia, typhoid bacillus, Bacillus paratyphosus B, shigella dysenteriae, Escherichia coli, green
Purulence bacillus, proteus, Bordetella pertussis, comma bacillus etc. have different degrees of inhibiting effect.Radix Paeoniae Alba is in vitro to violet hair
Tinea bacterium, Trichophyton concentricum, trichophyton schoenleini, microsporum audouini, microsporum ferrugineum, ulotrichy microspore's tinea
The dermatophytes such as bacterium, epidermophyton inguinale, epidermophyton rubrum and star-shaped nocardia also have different degrees of inhibiting effect.This
Outside, Chinese herbaceous peony decoction 1:40 in vitro has inhibiting effect to capital section 68-1 virus and herpesviral.Contained 1,2,3,4 in Chinese herbaceous peony,
Five galloyl glucose of 6- has antiviral activity；
Liver protection and detoxication: radix paeoniae alba extraction to Liver Damage in Rats caused by D-galactosamine and aflatoxin B1 with
ALT is increased, and increases to the gross activity of lactic dehydrogenase caused by the latter (SLDH) and its isodynamic enzyme, there is obvious inhibiting effect.With
Duck young bird aflatoxin B1 detoxification experiment shows that radix paeoniae alba extraction plays the role of destroying aflatoxin in a specified time.It is white
Chinese herbaceous peony ethanol extract has certain degradation to aspergillus flavus poison rope B1 in vitro.Radix paeoniae alba extraction 250mg/kg stomach-filling, to mouse
T-2 toxin poisoning has obvious detoxication.
Anti- mutagenesis and antitumor action: radix paeoniae alba extraction can interfere the enzymatic activity of S9 mixed liquor, and can make benzo Wang (BAP)
Metabolin inactivation and inhibit the mutagenesis of BAP.Gallic acid (GA) and five galloyl glucoses (PGG) can make BAP
Metabolin inactivation, PGG can inhibit the enzymatic activity of S9 mixed liquor.Show that Radix Paeoniae Alba is extracted with mouse P-388 leukaemia cell experiment
Object can enhance the antitumor action of mitomycin C, furthermore can still inhibit oligoleukocythemia caused by mitomycin C；
Other effects: Radix Paeoniae Alba ingredient Paeoniflorin member ketone 0.04% has pole to the neuromuscular junction of mouse phrenic nerve diaphragm
Change type inhibiting effect.Chinese herbaceous peony has inhibiting effect to aldose reductase (RLAR) activity of rat eye crystal in vitro, effectively
The inhibition of four-O- galloyl-beta-D-glucose of ingredient and five-O- galloyl-beta-D-glucoses, 1 μ g/ml to RLAR
Rate is respectively 77.6% and 61.0%.Chinese herbaceous peony treats diabetic neuropathy may be with its RLAR inhibiting effect to peripheral nerve
It is related.Radix Paeoniae Alba water extract 64mg/ml significantly inhibits effect to rat pancreatic amylase activity, concentration is≤16mg/ml when not
Enzyme activity is influenced, the potency for the isolated rat pancreatic acini secreting amylase that octapeptide cholecystokinin can but induced reduces by 10
Times, but do not influence the enzyme secretion of secretin stimulation, show Radix Paeoniae Alba can cholecystokinin on antagonism pancreatic acinar cell film by
Body.Radix paeoniae alba extraction is to enkephalin receptor, alpha-2-adrenoceptor, angiotensin-ii-receptor, beta-hydroxy-Beta-methyl glutaric acid
Coacetylase, complement system, cholecystokinin and the system converting enzyme of purine etc. have different degrees of inhibiting effect.Peony extract
The mast cell histamine release that 25mg/ml induces compound 48/80 has obvious inhibiting effect.
The physical and chemical of white peony identifies:
1, powder 0.5g of the present invention, adds water 10ml, boils, and filtration, filtrate adds ferric trichloride test solution 1 to drip, and generates black-and-blue
Precipitating checks tannin；
2, thin-layer chromatography takes this product powder 0.5g, adds ethyl alcohol 10ml, shakes 5min, filtration, and filtrate is evaporated, and residue adds ethyl alcohol
2ml makes to dissolve, as test solution.Paeoniflorin reference substance separately is taken, adds ethyl alcohol that solution of every 1ml containing 2mg is made, as control
Product solution.Each 4 μ l of above two solution is drawn, is put respectively on same silica gel g thin-layer plate, with chloroform-ethyl acetate-methanol-
Formic acid (40:5:10:0.2) is solvent, is unfolded, and takes out, dries, and is sprayed with 5% vanillin-sulfuric acid solution, it is aobvious that hot wind is blown to spot
Color is clear.In sample chromatogram, at the position corresponding to the chromatogram of the reference substance, identical bluish violet spot is shown.Quality mark sheet
Product must not be less than 2.0% containing Paeoniflorin (C23H28O11).
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of breed improvement method of Radix Paeoniae Alba, it is characterised in that: specific step is as follows for the modification method:
S1: single-lobe Radix Paeoniae Alba and more valve Radix Paeoniae Albas is selected to carry out the mixing breed of artificial pollination；
S2: with adopting with broadcasting after seed is mature, pressing line-spacing 5cm water furrow planting on furrow, and earthing 1cm sprays water 1 time for every 2 days, more than 30 days
It can emerge, after autumn or the 2nd year spring transplanted；
S3: ramificatiom breeding finally is carried out to plant bred seedling.
2. a kind of breed improvement method of Radix Paeoniae Alba according to claim 1, it is characterised in that: in the step S1, manually
Specific step is as follows for pollination:
S11: using single-lobe Radix Paeoniae Alba as male parent, more valve Radix Paeoniae Albas are as maternal, collection paternal pollen, by the paternal pollen hair of collection
Pen dips on maternal gynoecium, primary every 2~3d pollination, continuously pollinates 3~4 times；
S12: fresh idea is cut after 40~60d seed is mature, acquires seed after drying.
3. a kind of breed improvement method of Radix Paeoniae Alba according to claim 2, it is characterised in that: in the step S11, in people
Flower of female parent crown scissors should be gone to big portion by work when pollinating, retained 1~5cm of base portion, can not be injured maternal pistil stigma, and pollinating every time
Bagging afterwards extracts bag after last 1 pollination 1 week.
4. a kind of breed improvement method of Radix Paeoniae Alba according to claim 2, it is characterised in that: in the step S11, one day
In optimal pollination time be fine day 10~12 point, and extra flower is wiped out when fresh idea is aging after the 15~20d that pollinates,
To reduce nutrient consumption, increases sunlight and penetrate, it is mature to be conducive to seed development.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
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| CN101869051A (en) * | 2010-07-22 | 2010-10-27 | 甘肃省林业科学技术推广总站 | Hybridization breeding pollination method for peony |
| CN105145332A (en) * | 2015-07-08 | 2015-12-16 | 亳州市谯城区药用动植物研究所 | Multi-color paeonia sterniana fletcher breeding method |
| CN105325282A (en) * | 2015-09-28 | 2016-02-17 | 贺家振 | Oil-used paeonia lactiflora first-generation hybrid seed production method and seed uses |
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| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
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