CN108887171A - A kind of hybridizing method of Chinese herbaceous peony platymiscium - Google Patents
A kind of hybridizing method of Chinese herbaceous peony platymiscium Download PDFInfo
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- CN108887171A CN108887171A CN201810768094.5A CN201810768094A CN108887171A CN 108887171 A CN108887171 A CN 108887171A CN 201810768094 A CN201810768094 A CN 201810768094A CN 108887171 A CN108887171 A CN 108887171A
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- hybridization
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- chinese herbaceous
- promotes liquid
- herbaceous peony
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- General Health & Medical Sciences (AREA)
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention discloses a kind of hybridizing methods of Chinese herbaceous peony platymiscium.When the florescence, maternal just budding bud is selected, petal is peelled off, remove stamen, brush hybridization promotes liquid on column cap, then pollinates, bagging fixes paper bag, and paper bag is avoided to fall off.Using method of the invention, hybridization was completed at one day.Hybridization efficiency is high, reduces the risk of hybridization failure.Setting percentage reaches 14% or more.
Description
Technical field
The present invention relates to the hybridization field of plant more particularly to a kind of hybridizing methods of Chinese herbaceous peony platymiscium.
Background technique
Tree peony or when Chinese herbaceous peony hybridization pollination at present, using two-step method：The first step, to tree peony or Chinese herbaceous peony female parent bud it is in bud to
When putting, petal is peelled off, removes stamen, paper bag is covered, fixes paper bag with clip, paper clip or pin, paper bag is avoided to fall off.Second
Step, after 2-3 days, finds the last time and removes bud after stamen bagging, at this time secretion stigma mucus, take down paper bag, pollinates, and bagging is used
Paper bag after stapler bookbinding pollination, avoids paper bag from falling off.The method, which is divided to two days, to be completed, comparatively laborious, is also easy to omit；While two
Secondary operation increases the maternal chance exposed in air, is easy string powder, increases the risk of hybridization failure.
Therefore it is badly in need of a kind of hybridization pollination method that the simple and convenient used time is short.
Summary of the invention
The purpose of the present invention is to provide a kind of Paeonia plant hybridization pollinating methods that the simple and convenient used time is short.
To achieve the above object, the present invention provides a kind of hybridizing method of Chinese herbaceous peony platymiscium, which is characterized in that and method is,
When the florescence, maternal just budding bud being selected, petal is peelled off, removes stamen, brush hybridization promotes liquid on column cap, then pollinate, set
Bag, fixes paper bag, paper bag is avoided to fall off.
Further, hybridized when florescence temperature is lower than 25 DEG C.
Further, it is to brush hybridization on column cap using hairbrush to promote liquid that brush hybridization, which promotes liquid, on the column cap；Preferably, it adopts
Hybridization is brushed on column cap with the pen with hairbrush and promotes liquid.
Further, it is 40~80mg/L boric acid, 0.1~0.4g/L CaCl that the hybridization, which promotes liquid,2, 50~150g/L sugarcane
Sugar, pH 5.8~7.0；Preferably, it is 40~60mg/L boric acid, 0.1~0.3g/L CaCl that the hybridization, which promotes liquid,2, 80~
120g/L sucrose, pH 5.8~7.0；It is furthermore preferred that it is 50mg/L boric acid, 0.2g/L CaCl that the hybridization, which promotes liquid,2, 100g/
L sucrose, pH 6.0.
Further, described that paper bag is made to be fixed as binding using stapler.
Further, the Chinese herbaceous peony platymiscium is tree peony or Chinese herbaceous peony.
PH value is 5.8-7.0.The environment of meta-acid is conducive to pollen germination, facilitates cross-fertile.
Using method of the invention, hybridization was completed at one day.Hybridization efficiency is high, and same workload significantly improves hybridization number
Amount, the risk 50% to fail caused by reducing because of string powder.Setting percentage reaches 14% or more.
Specific embodiment
The embodiment of the present invention is described below in detail, the examples of the embodiments are intended to be used to explain the present invention, and cannot
It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, described according to the literature in the art
Technology or conditions or carried out according to product description.Reagents or instruments used without specified manufacturer is that can lead to
Cross the conventional products of commercially available acquisition.
The hybridizing method of Chinese herbaceous peony platymiscium selects maternal just budding bud, peels off petal, removes stamen, brush on column cap
Hybridization promotes liquid, then pollinates, bagging fixes paper bag, and paper bag is avoided to fall off.
Further, hybridized when florescence temperature is lower than 25 DEG C.
Further, it is to brush hybridization on column cap using hairbrush to promote liquid that brush hybridization, which promotes liquid, on the column cap；Preferably, it adopts
Hybridization is brushed on column cap with the pen with hairbrush and promotes liquid.
Further, it is 40~80mg/L boric acid, 0.1~0.4g/L CaCl that the hybridization, which promotes liquid,2, 50~150g/L sugarcane
Sugar, pH 6.0~7.0；Preferably, it is 40~60mg/L boric acid, 0.1~0.3g/L CaCl that the hybridization, which promotes liquid,2, 80~
120g/L sucrose, pH 6.0~7.0；It is furthermore preferred that it is 50mg/L boric acid, 0.2g/L CaCl that the hybridization, which promotes liquid,2, 100g/
L sucrose, pH 7.0.
Further, described that paper bag is made to be fixed as binding using stapler.
Further, the Chinese herbaceous peony platymiscium is tree peony or Chinese herbaceous peony.
Configuration hybridization promotes liquid
It is 50mg/L boric acid, 0.2g/L CaCl that hybridization, which promotes liquid A,2, 100g/L sucrose, pH 7.0.
It is 60mg/L boric acid, 0.4g/L CaCl that hybridization, which promotes liquid B,2, 150g/L sucrose, pH 6.0~7.0；
It is 70mg/L boric acid, 0.3g/L CaCl that hybridization, which promotes liquid C,2, 50g/L sucrose, pH 6.2；
It is 80mg/L boric acid, 0.2g/L CaCl that hybridization, which promotes liquid D,2, 120g/L sucrose, pH 5.8；
It is 40mg/L boric acid, 0.1g/L CaCl that hybridization, which promotes liquid E,2, 80g/L sucrose, pH 6.0；
By common acid such as hydrochloric acid, citric acid, common alkali such as sodium hydroxide etc. adjusts pH value.
Embodiment 1：The hybridization of Chinese herbaceous peony
By the end of April, Luoyang, when temperature is lower than 25 DEG C, o'clock in early morning 6-10 selects the maternal just budding bud of Chinese herbaceous peony, peels off
Petal removes stamen, is hybridized with writing brush in column cap brush and promotes liquid A, pollinates (male parent is tree peony), bagging, is awarded with stapler bookbinding
Paper bag after powder, avoids paper bag from falling off.Hybridization was completed at one day.Hybridization efficiency is high, reduces the risk of hybridization failure.It completes altogether
100 plants, final setting percentage reaches 14% or more.
Comparative example：By the end of April, Luoyang, o'clock in early morning 6-10 select the maternal just budding bud of Chinese herbaceous peony, peel off petal, remove
Stamen is pollinated (male parent is tree peony), bagging, with paper bag after stapler bookbinding pollination, paper bag is avoided to fall off.Hybridize complete at one day
At.Hybridization efficiency is high, reduces the risk of hybridization failure.100 plants are completed altogether, and final setting percentage is below 4%.
Embodiment 2：The hybridization of Chinese herbaceous peony
By the end of April, Luoyang, when temperature is lower than 25 DEG C, o'clock in early morning 6-10 selects the maternal just budding bud of Chinese herbaceous peony, peels off
Petal removes stamen, is hybridized with writing brush in column cap brush and promotes liquid B, pollinates (male parent is tree peony), bagging, is awarded with stapler bookbinding
Paper bag after powder, avoids paper bag from falling off.Hybridization was completed at one day.Hybridization efficiency is high, reduces the risk of hybridization failure.30 are completed altogether
Strain, final setting percentage reach 14% or more.
Embodiment 3：The hybridization of Chinese herbaceous peony
By the end of April, Luoyang, when temperature is lower than 25 DEG C, o'clock in early morning 6-10 selects the maternal just budding bud of Chinese herbaceous peony, peels off
Petal removes stamen, is hybridized with writing brush in column cap brush and promotes liquid C, pollinates (male parent is Chinese herbaceous peony), bagging, is awarded with stapler bookbinding
Paper bag after powder, avoids paper bag from falling off.Hybridization was completed at one day.Hybridization efficiency is high, reduces the risk of hybridization failure.30 are completed altogether
Strain, final setting percentage reach 14% or more.
Embodiment 4：The hybridization of tree peony
By the end of April, Luoyang, when temperature is lower than 25 DEG C, o'clock in early morning 6-10 selects the maternal just budding bud of tree peony, peels off
Petal removes stamen, is hybridized with writing brush in column cap brush and promotes liquid D, pollinates (male parent is tree peony), bagging, is awarded with stapler bookbinding
Paper bag after powder, avoids paper bag from falling off.Hybridization was completed at one day.Hybridization efficiency is high, reduces the risk of hybridization failure.30 are completed altogether
Strain, final setting percentage reach 14% or more.
Embodiment 5：The hybridization of tree peony
By the end of April, Luoyang, when temperature is lower than 25 DEG C, o'clock in early morning 6-10 selects the maternal just budding bud of tree peony, peels off
Petal removes stamen, is hybridized with writing brush in column cap brush and promotes liquid E, pollinates (male parent is tree peony), bagging, is awarded with stapler bookbinding
Paper bag after powder, avoids paper bag from falling off.Hybridization was completed at one day.Hybridization efficiency is high, reduces the risk of hybridization failure.30 are completed altogether
Strain, final setting percentage reach 14% or more.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case where can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Claims (6)
1. a kind of hybridizing method of Chinese herbaceous peony platymiscium, which is characterized in that method is, when the florescence, selects maternal a bud just ready to burst bud,
Petal is peelled off, stamen is removed, brush hybridization promotes liquid on column cap, then pollinates, bagging fixes paper bag, and paper bag is avoided to fall off.
2. hybridizing method as described in claim 1, which is characterized in that florescence temperature is hybridized when being lower than 25 DEG C.
3. hybridizing method as described in claim 1, which is characterized in that it is to be existed using hairbrush that brush hybridization, which promotes liquid, on the column cap
Brush hybridization promotes liquid on column cap；Preferably, hybridization is brushed on column cap using the pen with hairbrush and promotes liquid.
4. hybridizing method as described in claim 1, which is characterized in that the hybridization promotes liquid for 40~80mg/L boric acid, and 0.1
~0.4g/L CaCl2, 50~150g/L sucrose, pH 5.8~7.0；Preferably, it is 40~60mg/L boron that the hybridization, which promotes liquid,
Acid, 0.1~0.3g/L CaCl2, 80~120g/L sucrose, pH 5.8~7.0；It is furthermore preferred that the hybridization promotion liquid is
50mg/L boric acid, 0.2g/L CaCl2, 100g/L sucrose, pH 6.0.
5. hybridizing method as described in claim 1, which is characterized in that described that paper bag is made to be fixed as binding using stapler.
6. hybridizing method as described in claim 1, which is characterized in that the Chinese herbaceous peony platymiscium is tree peony or Chinese herbaceous peony.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601372A (en) * | 2019-01-02 | 2019-04-12 | 四川鑫沃康农业有限责任公司 | A kind of breed improvement method of Radix Paeoniae Alba |
| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
| CN113273490A (en) * | 2021-05-27 | 2021-08-20 | 洛阳农林科学院 | Method for overcoming incompatibility of distant hybridization stigma of plant |
| CN116439123A (en) * | 2023-03-15 | 2023-07-18 | 洛阳市农林科学院 | Artificial hybridization method for cultivating new yellow peony variety by interclass pilot pollination |
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| US6046391A (en) * | 1995-10-27 | 2000-04-04 | Chen; Dezhong | Parental combination method for breeding purple dotted peony |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109601372A (en) * | 2019-01-02 | 2019-04-12 | 四川鑫沃康农业有限责任公司 | A kind of breed improvement method of Radix Paeoniae Alba |
| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
| CN113273490A (en) * | 2021-05-27 | 2021-08-20 | 洛阳农林科学院 | Method for overcoming incompatibility of distant hybridization stigma of plant |
| CN116439123A (en) * | 2023-03-15 | 2023-07-18 | 洛阳市农林科学院 | Artificial hybridization method for cultivating new yellow peony variety by interclass pilot pollination |
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