CN103408355B - Method and special treatment agent for increasing pollination seed setting rate of dendrobium officinale and dendrobium moniliforme - Google Patents
Method and special treatment agent for increasing pollination seed setting rate of dendrobium officinale and dendrobium moniliforme Download PDFInfo
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- CN103408355B CN103408355B CN201310299149.XA CN201310299149A CN103408355B CN 103408355 B CN103408355 B CN 103408355B CN 201310299149 A CN201310299149 A CN 201310299149A CN 103408355 B CN103408355 B CN 103408355B
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Abstract
The present invention relates to a method and a special treatment agent for increasing pollination seed setting rates of dendrobium officinale and dendrobium moniliforme. The special treatment agent contains 0.4-0.8 mmol/L of Ca(NO3)2, 0.1-0.5 mmol/L of H3BO3, 50-90 g/L of sucrose, 10-30 mg/L of urea, 80-110 mg/L of gibberellin, and 1-4 mg/L of a 2,4-D aqueous solution. The method mainly comprises: selecting parent plants; selecting a pollination time and a flowering time of the pollinated flower; carrying out artificial pollination; carrying out a bagging treatment; and obtaining seeds of dendrobium officinale and dendrobium moniliforme. With the method and the special treatment agent, seed setting success rates of dendrobium officinale and dendrobium moniliforme can be significantly improved, planting yields can be increased, operations are convenient and easy to grasp, and the method and the special treatment agent can be popularized and applied in large scale artificial planting.
Description
Technical field
The present invention relates to and a kind ofly improve Herba Dendrobii, the dendrobium moniliformeSweet pollination method of setting percentage and special treating agent.
Background technology
Herba Dendrobii, has another name called Herba hedyotis costatae, is the orchid family Dendrobium Precious, Rare, Endangered endemic species.This raw lignicolous fragrant thoroughwort is a kind of time-honored rare traditional Chinese medicine, to be listed in first of " Chinese nine immortals grass " existing thousand, by " Chinese Pharmacopoeia " collection, to have distinguished health care drug effect, as: nourishing Yin and clearing heat, the beneficial stomach that promotes the production of body fluid, moistens the lung and relieve the cough.2010 editions Pharmacopoeias of the People's Republic of China are out single-row from the stem of noble dendrobium as newly-increased kind using Herba Dendrobii, have raising immune, anti-ageing, anti-curing oncoma and cardiovascular disorder and improving water flood, prevent and treat the functions such as illness such as cataract.
Dendrobium moniliformeSweet, has another name called Dendrobium Moniliforme, is the perennial herbaceous plant that grows nonparasitically upon another plant of the orchid family Dendrobium, and dry stem, as one of high-quality Chinese medicine stem of noble dendrobium cultivar origin, is often processed into " Tongpi Fendou ", has reinforcing stomach reg fluid, nourishing Yin and clearing heat function; Clinically hinder body fluid deficiency for the moon, dry polydipsia, food is few retches, abnormal heat after being ill, and order is secretly not clear waits disease.
Herba Dendrobii, dendrobium moniliformeSweet are as Self-Incompatibility plant, and naturally solid main dependence entomochory pollination, setting percentage is very low.Reasonable disposition must be suitable for pollinated variety or by florescence artificial pollination guarantee yield and quality aborning, but often because of manual pollination not in time or the reasons such as solid success ratio is low and cause the underproduction of pollinating.Therefore, need badly select rational pollination procedures and select effective special treating agent help manual pollination with arrives raising Herba Dendrobii, dendrobium moniliformeSweet output object.
Summary of the invention
For current manual pollination not in time or the reasons such as solid success ratio is low and cause the problem of the underproduction of pollinating, the object of the invention is to design and a kind ofly improve Herba Dendrobii, the method for dendrobium moniliformeSweet pollination setting percentage and special treating agent.
Improve a special treating agent for Herba Dendrobii, dendrobium moniliformeSweet pollination setting percentage, this special treating agent is for containing 0.4 ~ 0.8mmol/LCa (NO
3)
2, 0.1 ~ 0.5mmol/L H
3bO
3, 50 ~ 90g/L sucrose, 10 ~ 30mg/L urea, 80 ~ 110mg/L Plant hormones regulators,gibberellins, the aqueous solution of 1 ~ 4mg/L2,4-D.
Wherein, the preferred 0.5mmol/L ~ 0.7mmol/L Ca of the concentration of each composition (NO
3)
2, 0.2mmol/L ~ 0.4mmol/L H
3bO
3, 60g/L ~ 80g/L sucrose, 20 ~ 30mg/L urea, 90mg/L ~ 110mg/L Plant hormones regulators,gibberellins, the aqueous solution of 2mg/L ~ 4mg/L2,4-D.
Optimum concn is 0.6mmol/L Ca (NO
3)
2, 0.3mmol/L H
3bO
3, 70g/L sucrose, 25mg/L urea, 100mg/L Plant hormones regulators,gibberellins, the aqueous solution of 3mg/L2,4-D.
The invention also discloses the method improving Herba Dendrobii, dendrobium moniliformeSweet pollination setting percentage with described special treating agent, comprise the following steps:
1) parent chooses: choose same branch or same strain Herba Dendrobii, dendrobium moniliformeSweet spend mutual hybridization, to keep the good characteristic of kind;
2) to pollination time and choosing of pollinating that the flowers are in blossom and take time: between 7 o'clock to the 10 o'clock morning and 3 o'clock to 5 o'clock afternoon, the flower choosing Post flowering 3-6 days carries out manual pollination;
3) manual pollination: choose same branch or same strain Herba Dendrobii, dendrobium moniliformeSweet flower respectively as male parent and female parent, be put into by the pollen of male parent on maternal column cap, the special treating agent of pollinate described in rear 0.2ml ~ 0.3ml spreads upon on the column cap of female parent;
4) bagging process: after pollination, puts sulfuric acid paper bag, and is squirted, and is in moisturizing state 2 hours, within latter 2 days, extracts in pollination;
5), after solid success, extract the seed that growing way is poor, every clump of seed only leaves 5 ~ 6 seeds grown fine;
6) seed is obtained.
Above-mentioned a kind ofly improve Herba Dendrobii, the method for dendrobium moniliformeSweet pollination setting percentage and special treating agent, design objective is clear and definite, and scheme is reasonable.The present invention is by the activity of research Herba Dendrobii, dendrobium moniliformeSweet Post flowering column cap, with the nutrition promoted needed for germination of pollen tube, elongation, hormone, some trace elements, determine best pollination time and the optimum special treating agent of allotment, thus improving Herba Dendrobii, dendrobium moniliformeSweet setting percentage, Herba Dendrobii, dendrobium moniliformeSweet setting percentage are increased to 85%.Dedicated processes agent prescription of the present invention is reasonable, successful; And the inventive method is easy, easy handling.
Embodiment
The present invention is further illustrated below by way of experimental example.
Embodiment one: Herba Dendrobii kind 1 and dendrobium moniliformeSweet kind 1 manual pollination experiment:
1, Herba Dendrobii kind 1 is chosen and dendrobium moniliformeSweet product a kind arrange control group by table 1 and experimental group carries out manual pollination's experiment.
2, according to group process: control group Stochastic choice pollination time, flower carry out manual pollination, do not smear special treating agent, do not carry out bagging moisturizing process after pollination on maternal column cap.
3, treatment agent preparation: special treating agent is for containing 0.6mmol/L Ca (NO
3)
2, 0.3mmol/L H
3bO
3, 70g/L sucrose, 25mg/L urea, 100mg/L Plant hormones regulators,gibberellins, the aqueous solution of 3mg/L2,4-D.
4, experimental group process: select between 7 o'clock to the 10 o'clock morning and 3 o'clock to 5 o'clock afternoon experimental group, the flower choosing Post flowering 3-6 days carries out manual pollination.Then and put sulfuric acid paper bag smear the special treating agent of 0.2ml ~ 0.3ml after pollination at maternal column cap, and squirted, and be in moisturizing state 2 hours, within latter 2 days, extract in pollination.
5, add up the setting percentage of Herba Dendrobii kind 1 and dendrobium moniliformeSweet kind 1 control group and experimental group respectively, result is as shown in table 1,
Table 1 manual pollination setting percentage
Can find that experimental group setting percentage is up to more than 85%, apparently higher than control group by result in table.
Embodiment two: Herba Dendrobii kind 2 manual pollination tests:
Experimental procedure is as tested one, and wherein treatment agent is for containing 0.4mmol/L Ca (NO
3)
2, 0.1mmol/L H
3bO
3, 50g/L sucrose, 10mg/L urea, 80mg/L Plant hormones regulators,gibberellins, the aqueous solution of 1mg/L2,4-D.
Smear the special treating agent of 0.2ml at maternal column cap, bagging moisturizing 2 hours, within latter 2 days, extract in pollination.
Result is as shown in table 1, and experimental group setting percentage is apparently higher than control group.
Implement three: dendrobium moniliformeSweet kind 2 manual pollination tests:
Experimental procedure is as tested one, and wherein treatment agent is for containing 0.8mmol/L Ca (NO
3)
2, 0.5mmol/L H
3bO
3, 90g/L sucrose, 30mg/L urea, 110mg/L Plant hormones regulators,gibberellins, the aqueous solution of 4mg/L2,4-D.
Smear the special treating agent of 0.3ml at maternal column cap, bagging moisturizing 2 hours, within latter 2 days, extract in pollination.
Result is as shown in table 1, and experimental group setting percentage is apparently higher than control group.
Embodiment four: Herba Dendrobii kind 3 manual pollination tests:
Experimental procedure is as tested one, and wherein treatment agent is for containing 0.5mmol/L Ca (NO
3)
2, 0.2mmol/L H
3bO
3, 60g/L sucrose, 20mg/L urea, 90mg/L Plant hormones regulators,gibberellins, the aqueous solution of 2mg/L2,4-D.
Smear the special treating agent of 0.2ml at maternal column cap, bagging moisturizing 2 hours, within latter 2 days, extract in pollination.
Result is as shown in table 1, and experimental group setting percentage is apparently higher than control group.
Embodiment five: dendrobium moniliformeSweet kind 3 manual pollination tests:
Experimental procedure is as tested one, and wherein treatment agent is for containing 0.7mmol/L Ca (NO
3)
2, 0.4mmol/L H
3bO
3, 80g/L sucrose, 30mg/L urea, 110mg/L Plant hormones regulators,gibberellins, the aqueous solution of 4mg/L2,4-D.
Smear the special treating agent of 0.3ml at maternal column cap, bagging moisturizing 2 hours, within latter 2 days, extract in pollination.
Result is as shown in table 1, and experimental group setting percentage is apparently higher than control group.
Claims (4)
1. improving a special treating agent for Herba Dendrobii, dendrobium moniliformeSweet pollination setting percentage, it is characterized in that described special treating agent is for containing 0.4 ~ 0.8mmol/L Ca (NO
3)
2, 0.1 ~ 0.5mmol/L H
3bO
3, 50 ~ 90g/L sucrose, 10 ~ 30mg/L urea, 80 ~ 110mg/L Plant hormones regulators,gibberellins, the aqueous solution of 1 ~ 4mg/L2,4-D.
2. a kind of special treating agent improving Herba Dendrobii, dendrobium moniliformeSweet pollination setting percentage as claimed in claim 1, is characterized in that described special treating agent is for containing 0.5mmol/L ~ 0.7mmol/L Ca (NO
3)
2, 0.2mmol/L ~ 0.4mmol/L H
3bO
3, 60g/L ~ 80g/L sucrose, 20 ~ 30mg/L urea, 90mg/L ~ 110mg/L Plant hormones regulators,gibberellins, the aqueous solution of 2mg/L ~ 4mg/L2,4-D.
3. a kind of special treating agent improving Herba Dendrobii, dendrobium moniliformeSweet pollination setting percentage as claimed in claim 2, is characterized in that described special treating agent is for containing 0.6mmol/L Ca (NO
3)
2, 0.3mmol/L H
3bO
3, 70g/L sucrose, 25mg/L urea, 100mg/L Plant hormones regulators,gibberellins, the aqueous solution of 3mg/L2,4-D.
4. utilize claim 1 or the special treating agent described in 2 or 3 to improve a method for Herba Dendrobii, dendrobium moniliformeSweet pollination setting percentage, it is characterized in that comprising the following steps:
1) parent chooses: choose same branch or same strain Herba Dendrobii, dendrobium moniliformeSweet spend mutual hybridization, to keep the good characteristic of kind;
2) to pollination time and choosing of pollinating that the flowers are in blossom and take time: between 7 o'clock to the 10 o'clock morning and 3 o'clock to 5 o'clock afternoon, the flower choosing Post flowering 3-6 days carries out manual pollination;
3) manual pollination: choose same branch or same strain Herba Dendrobii, dendrobium moniliformeSweet flower respectively as male parent and female parent, be put into by the pollen of male parent on maternal column cap, the special treating agent of pollinate described in rear 0.2ml ~ 0.3ml spreads upon on the column cap of female parent;
4) bagging process: after pollination, puts sulfuric acid paper bag, and is squirted, and is in moisturizing state 2 hours, within latter 2 days, extracts in pollination;
5), after solid success, extract the seed that growing way is poor, every clump of seed only leaves 5 ~ 6 seeds grown fine;
6) seed of Herba Dendrobii, dendrobium moniliformeSweet is obtained.
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| CN103891602B (en) * | 2014-04-01 | 2015-06-24 | 浙江欧银农业发展有限公司 | Cross pollination method for dendrobium officinale |
| CN104488707A (en) * | 2014-12-03 | 2015-04-08 | 柳州市泓吉农业科技有限公司 | Culture medium for dendrobium candidum |
Citations (7)
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|---|---|---|---|---|
| CN86106655A (en) * | 1986-10-09 | 1987-06-17 | 广西平乐县化工厂 | Regulating agent for flowering |
| CN1050305A (en) * | 1989-09-14 | 1991-04-03 | 湖南农业科学院蔬菜研究所 | Day lily bud-keeping agent and operation technique thereof |
| CN1813520A (en) * | 2005-01-31 | 2006-08-09 | 云南农业大学 | High-yield breeding method of round-grained nonglutinous rice sterile line |
| CN101213940A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN102037967A (en) * | 2010-09-19 | 2011-05-04 | 浙江农林大学 | Method for improving Dendrobium officinale hybridization seed setting rate and special treating agent thereof |
| CN102618485A (en) * | 2012-04-01 | 2012-08-01 | 新疆农业大学 | Culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen |
| CN103120123A (en) * | 2013-02-04 | 2013-05-29 | 四川深达生物科技有限公司 | Culture medium for improving dendrobium officinale maturing rate and method for preparing culture medium |
-
2013
- 2013-07-16 CN CN201310299149.XA patent/CN103408355B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86106655A (en) * | 1986-10-09 | 1987-06-17 | 广西平乐县化工厂 | Regulating agent for flowering |
| CN1050305A (en) * | 1989-09-14 | 1991-04-03 | 湖南农业科学院蔬菜研究所 | Day lily bud-keeping agent and operation technique thereof |
| CN1813520A (en) * | 2005-01-31 | 2006-08-09 | 云南农业大学 | High-yield breeding method of round-grained nonglutinous rice sterile line |
| CN101213940A (en) * | 2008-01-18 | 2008-07-09 | 中国科学院昆明植物研究所 | Fast replication method for dendrobium |
| CN102037967A (en) * | 2010-09-19 | 2011-05-04 | 浙江农林大学 | Method for improving Dendrobium officinale hybridization seed setting rate and special treating agent thereof |
| CN102618485A (en) * | 2012-04-01 | 2012-08-01 | 新疆农业大学 | Culture medium for improving germination and growth of Kumaiti Armeniaca vulgaris Lam pollen |
| CN103120123A (en) * | 2013-02-04 | 2013-05-29 | 四川深达生物科技有限公司 | Culture medium for improving dendrobium officinale maturing rate and method for preparing culture medium |
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