CN106386461B - A kind of selection of early blossoming rip-panel morning glory - Google Patents
A kind of selection of early blossoming rip-panel morning glory Download PDFInfo
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- CN106386461B CN106386461B CN201610799676.0A CN201610799676A CN106386461B CN 106386461 B CN106386461 B CN 106386461B CN 201610799676 A CN201610799676 A CN 201610799676A CN 106386461 B CN106386461 B CN 106386461B
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- seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention discloses a kind of selection of early blossoming rip-panel morning glory, comprise the following steps：Spent with early blossoming Pharbitis purpurea as parent material A, rip-panel morning glory is parent material B, does two kinds of positive and negative Crossing systems of A × B and B × A and obtains morning glory hybrid seed AF respectively1And BF1；Using AF1× B and BF1The hybridization of × A two ways obtains AF respectively2And BF2Seed；With AF2The single plant of middle selection is female parent, with BF2The single plant of middle selection is male parent, carries out hybridization pollination, obtains cenospecies AF3；AF3Seed bagging is selfed, and obtains F4Seed；F4Seed was continuously selfed for 3-4 generations, obtained stable early blossoming rip-panel morning glory；The selection can obtain stable early blossoming rip-panel morning glory, and growth potential is prosperous, and fancy points is excellent, and florescence is more early, viewing period length, lead a cow flower variety prematurity 55 65 days than existing rip-panel currently on the market.
Description
Technical field
The invention belongs to technical field of plant cultivation, more particularly to a kind of selection of early blossoming rip-panel morning glory.
Background technology
Morning glory (Pharbitis nil (Linn.) Choisy) is that the annual tendril voluble herb of Convolvulaceae ipomoea is planted
Thing, sarmentum is elongated, and about 3~4 meters, how close complete stool is by short bristle.Leaf alternate, full edge or tool leaf are split.Cyme axillary, 1
To several.Corolla loudspeaker sample, the bright-coloured beauty of pattern.Morning glory originates in Ya Zhou torrid areas, now draws plant extensively in all over the world, and
Numerous garden-varieties are selected, flower pattern change is various, and pattern is rich and varied, and various regions are popular.Since morning glory easily cultivates, fits
Characteristic that should be wide and strong resistance has caused breeding scholar more and more to pay close attention to, cultivate more tool ornamental values, resistance and
Adaptable new varieties are the main breeding objectives of morning glory, and morning glory is able to widely applied premise and basis.Split
Valve morning glory is the kind with higher ornamental value, and flower pattern beauty is plentiful, petal multilayer cracking 15-20 pieces, currently on the market
Rip-panel morning glory is of less types, and existing rip-panel morning glory florescence is later, to be arrived in the Yangtze river basin by the end of October to 11
Just just bloom, the sight florescence is very short, and quantity of blooming is few, it is impossible to solid.Do not bloom in the outdoor cultivation of northern area, existing rip-panel
Flower variety of leading a cow can only be viewed and admired in the ground autumn culture such as Hainan and Yunnan.This just greatly suppresses this rip-panel type morning glory
Promote and apply.Therefore morning in florescence, adaptable rip-panel morning glory new varieties are selected, new varieties is pushed away in wide geographic area
Wide application has very big necessity.
The content of the invention
The present invention is in order to solve the deficiencies in the prior art, on the basis of existing fancy horticulture plant variety type is enriched,
A kind of selection of early blossoming rip-panel morning glory is provided, fancy points is good, and florescence is more early.
The present invention uses following technical scheme：
A kind of selection of early blossoming rip-panel morning glory, comprises the following steps：
(1) spent with early blossoming Pharbitis purpurea as parent material A, rip-panel morning glory is parent material B, is two kinds of A × B and B × A
Positive and negative Crossing system, obtains morning glory hybrid seed AF respectively1And BF1；
(2) second year sowed AF at the beginning of 7 months by the end of June1Seed, BF1Seed, parent material A seeds and parent material B kinds
Son, in AF1And BF1Post flowering chooses the plant of petal type and the petal type most proximity of parent material B, makees maternal material respectively
Material, using AF1× B and BF1The hybridization of × A two ways obtains AF respectively2And BF2Seed；
At the beginning of 7 months by the end of June, AF is sowed within (3) the 3rd years2Seed and BF2Seed, in AF2And BF2In each choose petal type
With parent material B is closest, the single plant in 25 days earlier than August florescence, respectively with AF2The single plant of middle selection is female parent, with BF2In
The single plant of selection is male parent, carries out hybridization pollination, obtains cenospecies AF3；
At the beginning of 7 months by the end of June, AF is sowed within (4) the 4th years3Seed, selection petal type and parent material B is closest, blooms
Phase blooms several more single plant baggings selfings earlier than August 20 days, disease-free, single plant, obtains F4Seed；
At the beginning of 7 months by the end of June, F is sowed within (5) the 5th years4Seed, with petal type with parent material B identical, florescence earlier than
August 20 days, disease-free, single plant are bloomed several more, setting percentages preferably breeding goal continuous 3-4 generations of selfing, obtain stable morning
Flower rip-panel morning glory；
The parent material B uses hole plate seedling growth, during long 4-6 pieces true leaf, shading treatment 12-18 days, during daily illumination
Between be 8-10h, then directly field planting arrive experimental plot, normal field management；
The female parent material chooses 10-15 plants of the single plant without any disease infection, and gynoecium hair is chosen on these plant
Educate normally, column cap does not have abortion, the flower of no brown stain；The male parent material is chosen without any disease infection, is bloomed earliest
15-20 plants of single plant, on these plant choose bloom when stamen development it is normal, the big flower of pollen amount.
Preferably, the parent material B and parent material A isolation implants.
Preferably, the female parent material is handled in advance, the next morning pollinates again；To female parent material
Processing procedure is：Second day bud that will be bloomed is selected, bud is torn from side, removes stamen, reclose bud, use stapler
Machine orders petal top.
Preferably, when handling female parent material, each single plant handles 8-10 bud.
Preferably, described parent material A, AF1Seed, BF1Seed, AF2Seed, BF2Seed and AF3Seed uses
Hole plate seedling growth, isolation implant；During the long 6-8 pieces true leaf of seedling, experimental plot, normal field management are arrived in field planting.
Preferably, parent material B shading treatments 15 days, daily light application time is 9h.
The selection can obtain stable early blossoming rip-panel morning glory, and growth potential is prosperous, and fancy points is excellent, florescence compared with
Early, viewing period length, leads a cow flower variety prematurity 55-65 days, invention increases morning glory than existing rip-panel currently on the market
Variety type, enrich the species of fancy horticulture plant, improve the effect that greening is viewed and admired, expanded the popularization of rip-panel morning glory
The region of application.
Brief description of the drawings
Fig. 1 is the selection schematic diagram of the embodiment of the present invention 1.
In figure, A spends for early blossoming Pharbitis purpurea, and B is rip-panel morning glory.
Embodiment
The present invention is further described in detail below in conjunction with the accompanying drawings, but not limited to this.
Embodiment 1
Parent material A：Morning glory (K11-40)；Roundleaf, pattern white, flower pattern are horn-like；
Parent material B:Morning glory (K11-01L), roundleaf, pattern white, flower pattern, rip-panel.
A kind of selection of early blossoming rip-panel morning glory, as shown in Figure 1, comprising the following steps：
1st, sowing early blossoming Pharbitis purpurea flower variety on June 25th, 2007 (parent material A)；Sowing on July 10 rip-panel is led a cow
Flower (parent material B).Directly field planting is to experimental plot when parent material hole plate seedling growth, parent material A grow to 6-8 piece true leaves, normally
Field management, August bloom and (bloom on the 12nd：For a colony, florescence also refers to initial bloom stage, that is, this colony
25% plant start to bloom).
During parent material B long 4-6 piece true leaves, shading treatment, processing mode 1 are carried out：Shading treatment 12 days, during daily illumination
Between be 8h；Processing mode 2：Shading 15 days, daily light application time are 9h；Processing mode 3：Shading treatment 18 days, during daily illumination
Between be 10h.Morning glory plant by above-mentioned processing, which is directly colonized, arrives experimental plot, parent material A, B isolation implant, normal field
Management, the rip-panel morning glory after processing bloom situation such as table 1.
Table 1
The rip-panel morning glory of processing 1, corolla is relatively small, and it is too high to do maternal column cap browning rate, does paternal pollen amount and does not fill
Foot, can also receive seed although doing and hybridizing, can substantially reduce Cross fertile rate；Although processing 3 rip-panel morning glory column cap and
Pollen is bloomed a little later all in normal range (NR), can just be done within 20th and be hybridized later to September, due to hybridization time relatively late,
Temperature is low, and setting percentage and seed quality are all not as processing 2, therefore the present embodiment is preferentially parent using the rip-panel morning glory of processing 2
This material.
2nd, parent material does two kinds of positive and negative Crossing systems of A × B and B × A respectively.As female parent material, choose without any disease
15 plants of the single plant of evil infection, selection Stamen development is normal on these plant, and column cap does not have abortion, the flower of no brown stain；As
Male parent material is chosen without any disease infection, 20 plants of the single plant for blooming earliest, the stamen hair when selection is bloomed on these plant
Educate normal, the big flower of pollen amount.
3rd, September 10 days, (calyx leaf length is less than flower to the bud that will be bloomed for second day by above-mentioned requirements selection female parent material
The half of flower bud total length, and heaved slightly among petal), bud is torn from side, the column cap of gynoecium is not touched, use tweezers
Careful removing stamen, then recloses bud, and petal top is ordered with stapler.Each single plant is processed as 10 buds.
4th, the male parent flower pollination that 11 morning of September is chosen, after pollination, unclamps female parent, before flower corolla
End is ordered, flower Natural closure, it is therefore prevented that the entrance of other pollen.Each processing listing mark.
5th, every 2 days, bud and flower are selected on Parent plant, on request, it is general each to plant the step of weight 3-4
Strain is persistently pollinated 5 times, the plant after hybridization pollination, and normal field liquid manure management and the prevention and control of plant diseases, pest control, pollination terminate 35 days
Left and right, when capsule becomes brown, can harvest the seed on maternal plant, morning glory cenospecies is obtained respectively after dry threshing
Sub- AF1And BF1。
6th, sowing on July 1st, 2008 AF1And BF1The seed of seed and parent material A and B.Hole plate seedling growth, AF1And BF1
During with parent material A long 6-8 piece true leaves, experimental plot is arrived in directly field planting, normal field management, and parent material A was in August 10 days or so
Bloom, AF1Bloom within 19th in August, BF1Bloom within 5th in September.The seedling of parent material B is identical with the processing 2 of above-mentioned steps 1, warp
The morning glory plant directly field planting for crossing processing is bloomed on the 2nd to experimental plot, September.
7th, the plant of petal type and the petal type most proximity of parent material B is chosen in AF1 and BF1 Post flowerings, respectively
Make female parent material, prepare AF1× B and BF1The combination of × A two ways.The selection mode of parent material is identical with step 2.Hybridization
Method repeats the step of 3-5.Harvest AF respectively then2And BF2Seed.
8th, sowing on July 1st, 2009 AF2And BF2Seed, hole plate seedling growth, during the long 6-8 pieces true leaf of seedling, directly field planting to examination
Test field, normal field management, AF2It is that August is bloomed on the 24th, BF2It is that August is bloomed on the 26th.In AF2And BF2In each choose petal type
It is closest with parent material B, the single plant in 26 days earlier than August florescence.
9th, respectively with AF2The single plant of middle selection is female parent, with BF2The single plant of middle selection is male parent, carries out hybridization pollination, parent
The selection mode of this material is identical with step 2.Hybridizing method repeats the step of 3-5, obtains cenospecies AF3.
10th, sowing on July 1st, 2010 AF3, hole plate seedling growth, during the long 6-8 pieces true leaf of seedling, experimental plot is arrived in directly field planting, just
Normal field management, plant bloom successively on the 18th in August, choose petal type and parent material B is closest, florescence is earlier than August
20 days, disease-free, single plant is bloomed several more single plant baggings selfings, obtains F4Seed.
11st, sowing on July 1st, 2011 F4For seed, identical with parent material B with petal type, florescence is earlier than August 20
Day, it is disease-free, single plant bloom it is several more, setting percentage preferably breeding goal continuously selfing 4 generations, obtain stable early blossoming rip-panel
Morning glory new varieties.
12nd, sowing early blossoming Pharbitis purpurea flower variety on July 1st, 2015 (parent material A)；Rip-panel morning glory (parent material
B) and new selection and breeding early blossoming rip-panel morning glory new varieties, hole plate seedling growth, directly field planting is to experimental plot when growing to 6-8 piece true leaves, just
Normal field management.Parent and the comparison of new varieties breeding time and fancy points, the results are shown in Table 2.
Table 2
By upper table as can be seen that with the early blossoming rip-panel morning glory new varieties of selection and breeding of the present invention, fancy points and parent B mono-
Cause, but florescence is advanced by 63 days than parent B, and single plant quantity of blooming substantially exceeds parent B.The flower variety of leading a cow of new selection and breeding, carries
The high ornamental value of rip-panel morning glory, extends viewing period, improves the effect that greening is viewed and admired.The early blossoming rip-panel of new selection and breeding is led
Ox flower variety can widen the commodity value promoted the use of region, lift the kind of rip-panel morning glory significantly.
Claims (6)
1. a kind of selection of early blossoming rip-panel morning glory, it is characterised in that comprise the following steps：
（1）Spent with early blossoming Pharbitis purpurea as parent material A, rip-panel morning glory is parent material B, is A × B and two kinds of B × A is positive and negative
Crossing system, obtains morning glory hybrid seed AF respectively1And BF1；
（2）Second year sowed AF at the beginning of 7 months by the end of June1Seed, BF1Seed, parent material A seeds and parent material B seeds,
AF1And BF1Post flowering chooses the plant of petal type and the petal type most proximity of parent material B, makees female parent material respectively, adopts
Use AF1× B and BF1The hybridization of × A two ways obtains AF respectively2And BF2Seed；
（3）At the beginning of 7 months by the end of June, AF is sowed within 3rd year2Seed and BF2Seed, in AF2And BF2In each choose petal type and parent
Material B is closest, the single plant in 25 days earlier than August florescence, respectively with AF2The single plant of middle selection is female parent, with BF2Middle selection
Single plant is male parent, carries out hybridization pollination, obtains cenospecies AF3；
（4）At the beginning of 7 months by the end of June, AF is sowed within 4th year3Seed, choose petal type and parent material B is closest, florescence earlier than
August 20 days, disease-free, single plant are bloomed several more single plant baggings selfings, obtain F4Seed；
（5）At the beginning of 7 months by the end of June, F is sowed within 5th year4Seed, with petal type with parent material B identical, florescence earlier than August 20
Several more, setting percentages preferably breeding goal of blooming day, disease-free, single plant was continuously selfed for 3-4 generations, obtains stable early blossoming and splits
Valve morning glory；
The parent material B uses hole plate seedling growth, and during long 4-6 pieces true leaf, shading treatment 12-18 days, daily light application time is
8-10h, then directly experimental plot, normal field management are arrived in field planting；
The female parent material chooses 10-15 plants of the single plant without any disease infection, and Stamen development is being chosen just on these plant
Often, column cap does not have abortion, the flower of no brown stain；The male parent material is chosen without any disease infection, earliest list of blooming
15-20 plants of strain, when selection is bloomed on these plant, stamen development is normal, the big flower of pollen amount.
A kind of 2. selection of early blossoming rip-panel morning glory according to claim 1, it is characterised in that parent's material
Expect B and parent material A isolation implants.
A kind of 3. selection of early blossoming rip-panel morning glory according to claim 1, it is characterised in that the maternal material
Material is handled in advance, and the next morning pollinates again；Processing procedure to female parent material is：Selection will bloom for second day
Bud, tear bud from side, remove stamen, reclose bud, petal top is ordered with stapler.
4. the selection of a kind of early blossoming rip-panel morning glory according to claim 3, it is characterised in that pre- to female parent material
When first being handled, each single plant handles 8-10 bud.
A kind of 5. selection of early blossoming rip-panel morning glory according to claim 1, it is characterised in that parent's material
Expect A, AF1Seed, BF1Seed, AF2Seed, BF2Seed and AF3Seed uses hole plate seedling growth, isolation implant；The long 6-8 pieces of seedling
During true leaf, experimental plot, normal field management are arrived in field planting.
A kind of 6. selection of early blossoming rip-panel morning glory according to claim 1, it is characterised in that parent's material
Expect B shading treatments 15 days, daily light application time is 9h.
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