CN101869051A - Hybridization breeding pollination method for peony - Google Patents
Hybridization breeding pollination method for peony Download PDFInfo
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- CN101869051A CN101869051A CN 201010233068 CN201010233068A CN101869051A CN 101869051 A CN101869051 A CN 101869051A CN 201010233068 CN201010233068 CN 201010233068 CN 201010233068 A CN201010233068 A CN 201010233068A CN 101869051 A CN101869051 A CN 101869051A
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Abstract
The invention relates to a hybridization breeding pollination method for peony, which comprises the following steps of: a, selecting a female parent and a male parent according to a breeding target; b, carrying out artificial castration on the female parent; c, collecting pollen; d, carrying out artificial pollination, wherein a pollination method adopts a method for bending stigmas to dip the pollen; e, bagging and marking; and f, harvesting and sowing seeds, wherein the seeds blossom out successively after being sown for 5 to 6 years. In the method, artificial pollination is carried out in time by a simple and easy method so as to obtain more hybrid progenies and quicken the directed hybridization breeding process of the peony. The adopted method for bending the stigmas to dip the pollen not only solves the problem of easy mixing of the pollen and avoids mechanical damage of pollination tools to the stigmas in the pollinating process, but also can save masses of pollen, guarantee the purity of the pollen of the male parent and improve the maturing rate of hybridization, particularly distant hybridization. The method has the advantages of simple operation, easy popularization and capability of greatly improving the working efficiency of the breeding industry of the peony and similar plants.
Description
Technical field
The present invention relates to plant hybridization breeding field, is a kind of hybridization breeding pollination method for peony specifically.
Background technology
The artificial hybridization breeding is an improvement plant genetic characteristic, the important channel of cultivating new varieties or newtype, and it can break the boundary between species to a certain extent, impels the gene exchange between different plant species, thereby forms new species.The personnel that are engaged in peony research both at home and abroad and produce have carried out century-old above artificial hybridization breeding, have cultivated a large amount of new varieties.But because peony character inheritance variation law is lacked intensive research, biological engineering's practice has blindness, simultaneously, traditional pollinating method mostly is with instruments such as writing brush or cotton swabs and dips in the pollen of getting male parent, spread upon on the maternal column cap, have problems such as pollen easily mixes, column cap easy damaged in the pollinating process, operating efficiency is lower.
Summary of the invention
Technical problem to be solved by this invention provides a kind of hybridization breeding pollination method for peony, overcome that the pollen that traditional pollinating method exists easily mixes, the column cap easy damaged in pollinating process, problems such as operating efficiency is lower, by selecting suitable parent, in good time artificial emasculation, the simple more hybrid generations of acquisition such as the in good time artificial pollination of method of employing, accelerate the process of peony judicious mating breeding.
The present invention solves its technical problem by following technical proposals:
A. select good maternal of merit, robust growth, stigma development according to breeding objective and have the maternal proterties that needs improvement and pollen vigor height, male parent that the loose powder amount is big;
B. maternal artificial emasculation is selected robust growth, and well-developed female parent is castrated the Hua Chukai pedestrian worker that advances; After the castration petal is restored, in time put the sulfuric acid paper bag, prevent that non-purpose pollen from importing into;
C. suitable paternal plant is selected in the pollen collection, before Hua Chukai, takes out pollen with tweezers, is placed on the paper, dries naturally, and it is standby that pollen is become scattered about on the paper;
D. the artificial pollination maternal plant is castrated the back bagging, pollinated in the 2nd～3 day, pollinating method adopts the column cap bending to dip in the powder method: take off the sulfuric acid paper bag, without pollinating tool, direct gently the dipping in ripe column cap in the crooked slightly back of fresh idea is placed on the pollen that scatters on the paper, is stained with column cap up to pollen;
E. after the pollination of bagging and mark, seal bagging immediately and indicate hybrid combination, remove the sulfuric acid paper bag after the week of pollinating;
F. after the mid or late August seed maturity is treated in the sowing of gathering, gather, in time dry in the shade and strip out seed, be seeded into immediately in the river sand, be transplanted to the land for growing field crops after 2 years, bloom successively after generally sowing 5～6 years by different hybrid combination.
Emasculating method described in the above-mentioned steps b is: select well-developed bud on the maternal plant, strip off sepal and petal take out flower pesticide gently with tweezers, do not injure column cap, and it is clean that flower pesticide keeps.
The present invention accelerates tree peony judicious mating breeding process by selecting suitable parent, in good time artificial emasculation, the simple more hybrid generations of acquisition such as the in good time artificial pollination of method of employing.The problem that pollen easily mixed when " the powder method is dipped in the column cap bending " of adopting in tree peony crossbreeding work both overcome pollination; Pollinating tool is to the mechanical damage of column cap when having avoided pollination simultaneously; And can save a large amount of pollen, and guaranteed the purity of paternal pollen, improved and hybridized especially distant hybridization ripening rate.This method is simple to operate, is easy to promote, and can greatly improve the operating efficiency to the similar breeding industry of peony.
Embodiment
Below in conjunction with embodiment the present invention is described in further details.
Embodiment 1
(1) bright-coloured and have improved seeds of purple plague purpura elegance for colors such as orange, the redness of seed selection, it is maternal to select the wild ancestor Paeonia delavayi to do, and main proterties is: flower is little, and flower peony, petal base portion do not have spot, there are side bud, an inflorescence that 8 flowers, camellia odor type are arranged at most.With wild Paeonia papaveracea Wen County population is male parent, and main proterties is: flower is big, floral white, petal base portion tool purple plague purpura, and no side bud, flower delicate fragrance, the loose powder amount is big, pollen vigor height;
(2) maternal artificial emasculation is selected robust growth, and well-developed female parent is selected the well-developed bud of Paeonia delavayi before Hua Chukai, strip off sepal and petal take out flower pesticide gently with tweezers, note not injuring column cap, after the castration petal is restored, put the sulfuric acid paper bag;
(3) Wen County Paeonia papaveracea pollen is gathered in the pollen collection, before bud is just opened, takes out pollen with tweezers, is placed on the paper, dries naturally, and it is standby that pollen is become scattered about on the paper;
(4) artificial pollination is after Paeonia delavayi is castrated bagging the 2nd day, takes off the sulfuric acid paper bag, the crooked slightly back of fresh idea is directly gently dipped in the column cap of Paeonia delavayi maturation be placed on Wen County Paeonia papaveracea pollen that scatters on the paper, is stained with column cap up to pollen;
(5) after the pollination of bagging and mark, seal bagging immediately and indicate hybrid combination, remove the sulfuric acid paper bag after the week of pollinating.
(6) gather and manage with delicacy after the sowing pollination, treat to gather behind seed maturity mid-August, in time dry in the shade and strip out seed, the statistics ripening rate is seeded in the river sand immediately, carries out mark, and the back statistics emergence rate of emerging was transplanted to the land for growing field crops after 2 years, sowed and bloomed successively after 5 years.
Embodiment 2
(1) be seed selection pattern novelty, the fragrance of a flower is peculiar, the polyphyll improved seeds of longer blooming period, and it is maternal to select the wild ancestor Paeonia delavayi to do, and main proterties is: flower is little, the single-lobe type, the flower peony has side bud, an inflorescence that 8 flowers are arranged at most, the camellia odor type.With Central Plains tree peony kind ' Corallium Japonicum Kishinouye platform ' is male parent, and main proterties is: flower is big, and is crown-shaped, the flower kermes, and no side bud, flower delicate fragrance, the loose powder amount is bigger, pollen vigor height;
(2) maternal artificial emasculation is selected robust growth, and well-developed Paeonia delavayi plant selects well-developed bud before Hua Chukai, strip off sepal and petal take out flower pesticide gently with tweezers, note not injuring column cap, after the castration petal is restored, put the sulfuric acid paper bag;
(3) Central Plains tree peony kind ' Corallium Japonicum Kishinouye platform ' pollen is gathered in the pollen collection.Before bud is just opened, take out pollen with tweezers, be placed on the paper, dry naturally, it is standby that pollen is become scattered about on the paper; For improving the distant hybridization ripening rate, repeat pollination;
(4) artificial pollination is after Paeonia delavayi is castrated bagging the 3rd day, takes off the sulfuric acid paper bag, the crooked slightly back of fresh idea is directly gently dipped in the column cap of Paeonia delavayi maturation be placed on Central Plains tree peony kind ' Corallium Japonicum Kishinouye platform ' pollen that scatters on the paper, is stained with column cap up to pollen;
(5) after the pollination of bagging and mark, seal bagging immediately and indicate hybrid combination, remove the sulfuric acid paper bag after the week of pollinating.
(6) gather and manage with delicacy after the sowing pollination, treat to gather behind the late August seed maturity, in time dry in the shade and strip out seed, the statistics ripening rate is seeded in the river sand immediately, carries out mark, and the back statistics emergence rate of emerging was transplanted to the land for growing field crops after 2 years, sowed and bloomed successively after 6 years.
Claims (3)
1. hybridization breeding pollination method for peony is characterized in that adopting the column cap bending to dip in the powder method and operates by following step:
A. according to breeding objective select that proterties is good, that robust growth, stigma development are good is maternal and have the maternal proterties that needs improvement and pollen vigor height, a male parent that the loose powder amount is big;
B. maternal artificial emasculation is selected robust growth, and well-developed maternal plant is castrated the Hua Chukai pedestrian worker that advances; After the castration petal is restored, in time put the sulfuric acid paper bag, prevent that non-purpose pollen from importing into;
C. suitable paternal plant is selected in the pollen collection, before bud is just opened, takes out pollen with tweezers, is placed on the paper, dries naturally, and it is standby that pollen is become scattered about on the paper;
D. the artificial pollination maternal plant is castrated the back bagging, pollinated in the 2nd～3 day, pollinating method adopts the column cap bending to dip in the powder method: take off the sulfuric acid paper bag, without pollinating tool, direct gently the dipping in ripe column cap in the crooked slightly back of fresh idea is placed on the pollen that scatters on the paper, is stained with column cap up to pollen;
E. after the pollination of bagging and mark, seal bagging immediately and indicate hybrid combination, remove the sulfuric acid paper bag after 1 week of pollinating;
F. after the mid or late August seed maturity is treated in the sowing of gathering, gather, in time dry in the shade and strip out seed, be seeded into immediately in the river sand, be transplanted to the land for growing field crops after 2 years, sow and bloom successively after 5～6 years by different hybrid combination.
2. a kind of hybridization breeding pollination method for peony according to claim 1, it is characterized in that the emasculating method described in the step b is: select well-developed bud on the maternal plant, strip off sepal and petal take out flower pesticide gently with tweezers, do not injure column cap, it is clean that flower pesticide keeps.
3. a kind of hybridization breeding pollination method for peony according to claim 1 is characterized in that described pollination can repeat pollination.
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| CN 201010233068 CN101869051A (en) | 2010-07-22 | 2010-07-22 | Hybridization breeding pollination method for peony |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155856A (en) * | 2013-04-12 | 2013-06-19 | 洛阳农林科学院 | Artificial hybridization method for improving peony breeding fecundity |
| CN105145332A (en) * | 2015-07-08 | 2015-12-16 | 亳州市谯城区药用动植物研究所 | Multi-color paeonia sterniana fletcher breeding method |
| CN108157167A (en) * | 2018-02-11 | 2018-06-15 | 青岛农业大学 | Improve the method that oil uses tree peony ' Feng Dan ' seed alpha-linolenic acid content |
| CN108887171A (en) * | 2018-07-13 | 2018-11-27 | 洛阳农林科学院 | A kind of hybridizing method of Chinese herbaceous peony platymiscium |
| CN109601372A (en) * | 2019-01-02 | 2019-04-12 | 四川鑫沃康农业有限责任公司 | A kind of breed improvement method of Radix Paeoniae Alba |
| CN111771592A (en) * | 2020-07-14 | 2020-10-16 | 广西壮族自治区农业科学院 | Method for improving fruit setting rate of exocarpium citri rubrum |
| CN111903511A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method suitable for crossbreeding peony for mild saline-alkali soil oil |
| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
| CN112075339A (en) * | 2020-09-30 | 2020-12-15 | 北京林业大学 | Method for cultivating peony polyploids by efficiently inducing polyploid pollen |
Citations (2)
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| CN1130462A (en) * | 1995-10-27 | 1996-09-11 | 陈德忠 | Parent combination process for artificially culturing paeonia suffruticosa |
| CN1947496A (en) * | 2005-10-10 | 2007-04-18 | 北京林业大学 | Parents composition method for distant hybrid of peony and Chinese herbaceous peony |
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- 2010-07-22 CN CN 201010233068 patent/CN101869051A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1130462A (en) * | 1995-10-27 | 1996-09-11 | 陈德忠 | Parent combination process for artificially culturing paeonia suffruticosa |
| CN1947496A (en) * | 2005-10-10 | 2007-04-18 | 北京林业大学 | Parents composition method for distant hybrid of peony and Chinese herbaceous peony |
Non-Patent Citations (2)
| Title |
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| 《中国优秀硕士学位论文全文数据库》 20091115 王越岚 "牡丹的杂交育种及组间杂种育性的研究" 第21页 1－3 , 2 * |
| 《中国花卉报》 20100512 无 "牡丹新品何以竞放陇原" 1－3 , 2 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103155856A (en) * | 2013-04-12 | 2013-06-19 | 洛阳农林科学院 | Artificial hybridization method for improving peony breeding fecundity |
| CN103155856B (en) * | 2013-04-12 | 2014-08-13 | 洛阳农林科学院 | Artificial hybridization method for improving peony breeding fecundity |
| CN105145332A (en) * | 2015-07-08 | 2015-12-16 | 亳州市谯城区药用动植物研究所 | Multi-color paeonia sterniana fletcher breeding method |
| CN108157167A (en) * | 2018-02-11 | 2018-06-15 | 青岛农业大学 | Improve the method that oil uses tree peony ' Feng Dan ' seed alpha-linolenic acid content |
| CN108887171A (en) * | 2018-07-13 | 2018-11-27 | 洛阳农林科学院 | A kind of hybridizing method of Chinese herbaceous peony platymiscium |
| CN109601372A (en) * | 2019-01-02 | 2019-04-12 | 四川鑫沃康农业有限责任公司 | A kind of breed improvement method of Radix Paeoniae Alba |
| CN111771592A (en) * | 2020-07-14 | 2020-10-16 | 广西壮族自治区农业科学院 | Method for improving fruit setting rate of exocarpium citri rubrum |
| CN111903511A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method suitable for crossbreeding peony for mild saline-alkali soil oil |
| CN111903512A (en) * | 2020-09-09 | 2020-11-10 | 江苏富芍生物科技有限公司 | Method for cross breeding of high-yield oil peony in desert area |
| CN112075339A (en) * | 2020-09-30 | 2020-12-15 | 北京林业大学 | Method for cultivating peony polyploids by efficiently inducing polyploid pollen |
| CN112075339B (en) * | 2020-09-30 | 2021-12-14 | 北京林业大学 | Method for cultivating peony polyploids by efficiently inducing polyploid pollen |
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Application publication date: 20101027 |