CN104025998B - Utilize the method for hybridized induction monoploid selecting and breeding corn inbred line - Google Patents
Utilize the method for hybridized induction monoploid selecting and breeding corn inbred line Download PDFInfo
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Abstract
The invention discloses a kind of method utilizing hybridized induction monoploid selecting and breeding corn inbred line, belong to crop breeding method field.The inventive method be utilize lucky high lure be No. 31 to the basic population or the crossbreed pollination that meet breeding objective, select aleurone layer top purple, the seed that plumule point marks without purple etc.; Then Natural double or artificial doubling are carried out to resulting materials, then carry out selfing; Then select Posterity phenotype neat and consistent, do not have separative head progeny row, be new inbred line.The inventive method haploid-induction is high, can reach more than 13%; Secondly, selected marker is obvious, and screening is easy and accuracy is high; In addition, in the present invention, lucky high the luring of induction system used is No. 31 proper heights, and flowering time is long and pollen amount is large, can carry out spontaneous pollination induction monoploid, remove artificial pollination from, not only save labour, and be applicable to the operation carrying out large-scale groups; The inventive method also has that breeding efficiency is high, program is simple, the fireballing feature of breeding.
Description
Technical field
The invention belongs to the method for crop breeding, relate in particular to the method utilizing hybridized induction monoploid selecting and breeding corn inbred line.
Background technology
Breeding is a periodically long job, and be bred as the time that a kind generally needs 10-15, not only workload is large, time-consuming, effort, but also may be long because lasting, the breeding objective formulated before 10 years, after making great efforts for many years, obtain the requirement that result has often been not suitable with market.Therefore, shortening breeding time, accelerating breeding speed is the target that breeding men lay siege to always, for this reason, propose many methods, as utilized flower pesticide or pollen cultures Haploid production, the method then doubling to be bred as inbred line can shorten breeding time greatly, once once by as on the make method, but facts have proved, because anther cultural difficulty is very large, technical sophistication, efficiency are not high, are difficult to extensive use in practice.
Nineteen fifty-nine, Univ Minnesota-Twin Cities USA CoeEH finds that corn single times of reproduction induction is that Stock6 passes through hybridization and can induce female parent material Haploid production, incorporation of markings is selected, and makes to utilize hybridized induction system to induce offspring to form monoploid and carries out haploid breeding and become possibility.Along with the development of corn breeding techniques, hybridized induction method for breeding haploidy pay attention to by breeding man, become emphasis and the focus of current international breeding circle research, and from Stock6, derived some new induction systems by the method for crossbreeding and improvement, as the SW14(Lashermes of France, 1988), the Krasnodar(Shatskaya of the former Soviet Union, 1994) and the ZMS(ChalykST of Moldova, 1994), the KWS etc. of Germany.China Agricultural University has utilized Stock6 to cultivate, and agricultural university is high lures No. 1 (Liu Zhizeng etc., 2000; Chen Shaojiang etc., 2003).The value of these induction systems greatly exceed Stock6.
Patent application " a kind of utilize the method for hybridized induction parthenocarpy material selective breeding corn variety " (application number is: 200610080832.4) disclose Jilin Academy of Agricultural Science utilize M278 × Stock6 combine based on colony, adopt pedigree method to carry out selfing, the test cross seed selection of 6 generations continuously.In each generation, is chosen as master with haploid-induction, seed Navajo purple plague purpura mark (seed top aleurone layer is with purple speckle), take into account plant ABPl(plant purple simultaneously, controlled by A1A2C2BPl complementary gene, show as dominant for most corn material) mark, pollen amount, fecundity and disease resistance successive selection.The average inductivity of final incubation reaches 10.4%, markup good, head progeny row monosystem that Comprehensive Traits is excellent, names that to lure for Ji is high be No. 3.Lucky high lure be No. 3 with Navajo dominant inheritance mark, i.e. purple aleurone layer and purple plumule.With Ji high lure be No. 3 give other corn material pollination after, normal dliploid show as purple aleurone layer and purple plumule point, the leaf sheath purple of plant, and the parthenocarpy seed aleurone layer purple that induction produces, plumule point is colourless, and plant leaf sheath is green, can confirm with this mark.The corn haploid induction line that the inductivity of lucky high domestic that select, the open report that lures that to be No. 3 be is the highest, is widely used.But lucky high luring is No. 3 and also there is shortcoming miscellaneous in various degree: (1) plant is short and small, can not be used to carry out spontaneous pollination Haploid production in independent field.In order to overcome this shortcoming, people are hybridized between by induction being often.But other some problems may appear in the induced material of hybridization generation, such as: reduce haploid-induction and the expression changing marker gene.R1-nj marker gene (NandaandChase, 1966) (purple scultellum and purple aleurone layer apical cap) is widely used in screening monoploid from dry seeds, but, in the induced material that hybridization produces, the expression of this gene is very large by maternal influence, sometimes differentiate that monoploid may become very difficult, or even impossible, especially there is suppressor (C1-I) to deposit in case at female parent.(2) seed speckle mark is inconsistent to the performance of different female parent material, increases the selection difficulty of monoploid seed.Therefore, cultivating new high-frequency induction system is the prerequisite of carrying out this work.
Summary of the invention:
The object of the invention is to provide the method utilizing hybridized induction single times of selecting and breeding corn inbred line.The method procedure of breeding is simple, breeding speed is fast and corn inbred line that is that cultivate isozygotys completely.
The present invention utilizes the method for hybridized induction monoploid selecting and breeding corn inbred line, comprises the steps:
(1), with high the luring of Ji be No. 31 and make male parent to meeting the basic population of breeding objective or crossbreed pollination, gather in the crops maternal fruit ear; Therefrom screen aleurone layer top purple, plumule point is without purple, and embryo face is less, triangular in shape, the seed of the comparatively dark mark of depression;
(2), to step (1) obtain material and carry out artificial doubling or Natural double, the shooting stage removes leaf sheath and blade is the plant of purple; Selfing is carried out to the plant that can normally bloom after doubling, according to individual plant results self-fertility seed;
(3), plant step (2) gained seed, select characters of progenies performance neat and consistent, do not have separative head progeny row, be new corn inbred line.
Ji described in said method is high to lure and is No. 31 and belongs to Zea maize seed (zeamaysL.), for the high-frequency haploid inducing line of Jilin Academy of Agricultural Science seed selection, be preserved in China typical culture collection center (preservation place is: Hubei China province wuchang, wuhan Luo Jia Shan Wuhan University) on June 3rd, 2010, deposit number is: CCTCCNo:P201008
Natural double described in said method refers under field conditions (factors), and monoploid seed or the intracellular chromosome doubling of seedling plants become normal diploid.
Artificial doubling described in said method refers to utilize colchicine solution process monoploid seed or seedling, makes its endocellular chromosome double to become normal dliploid.
Basic population described in said method refers to by multiple inbred line or kind after mixed pollination, the maize population of formation.This kind of groups can make multiple beneficial gene be polymerized, and merit is accumulated, and disease resistance is good, Comprehensive Traits is excellent, is therefore used as the population material of Breeding of Inbred Lines.
The material source of above-mentioned basic population also can be various excellent farm varieties etc.
Crossbreed described in said method refers to single cross hybrid, triple hybrid or double cross hybrid etc.
Meet breeding objective described in said method, according to the common understanding of those skilled in the art, refer to that the aspects such as economical character, high-yield character, disease resistance trait and degeneration-resistant proterties, quality trait are excellent.
Utilize the corn inbred line that produces in said method to cultivate the method for corn hybrid seed, exactly with the corn inbred line cultivated in said method for one of parent, with other hybridizations between selfed lines, be bred as new crossbreed.
The present invention utilizes hybridized induction can this method Breeding of Inbred Lines of Haploid production seed, application the present inventor free high-frequency hybridized induction list doubly solid induction system---lucky high luring is the pollen of No. 31 (inductivity is up to more than 13%), to excellent basic population and crossbreed pollination, the seed obtained, differentiated by chain mark property, obtain monoploid seed and plant, then become dliploid, the homozygous inbred lines of selfed breeding Cheng Xin through artificial or Natural double.Realize gametophyte to select, the corn inbred line that quick breeding is new, creates new germ plasm resource, general shortening inbred line breeding time 3-4.Lucky high to lure that to be No. 31 be by improveing lucky high luring be that No. 3 seed selections form, and hybridization haploid-induction reaches 13.67%, higher than Ji high lure be No. 3 10.40%, and proper height, can be used to carry out spontaneous pollination Haploid production in independent field; The speckle mark of seed is also more obvious, lucky high luring is that the seed speckle mark of No. 3 is inconsistent to the performance of different female parent material, increase the selection difficulty of monoploid seed, and lucky high luring is No. 31 selections strengthening seed speckle mark, good seed speckle is had to mark to different female parent material.
Lucky high luring is the source of No. 31:
2003, with Ji high lure be No. 3 for maternal, material based on the crossbreed that Stock6/M278-8-9-1-6 obtains for paternal hybrid, adopt pedigree method, be chosen as master with haploid-induction, seed Navajo purple plague purpura mark (seed top aleurone layer is with purple speckle), carry out individual plant mensuration, through continuous selfing and the test cross of 6 generations, take into account plant ABPl mark, pollen amount, fecundity and disease resistance to select, lucky high the luring of final incubation high-frequency monoploid is No. 31 simultaneously.Lucky high luring be No. 31 variable rate technology is plant purple, and blade purple, flower pesticide purple, pollen amount is large, and flowering time is long.Particularly lucky high luring is No. 31 and has more significantly seed Navajo genetic marker, i.e. purple aleurone layer and purple embryo labels than other induction frenulums.With Ji high lure be No. 31 give other corn material pollination after, normal dliploid show as purple aleurone layer and purple plumule point, the leaf sheath purple of plant, and the parthenocarpy seed aleurone layer purple that induction produces, plumule point is colourless, and plant leaf sheath is green, can confirm with this mark.
Compared with prior art, the advantage that the present invention has and beneficial effect: the inductivity of (1), the inventive method haploid inducing line used is high, the present invention's Ji used is high to lure is that the haploid-induction of No. 31 is on average more than 13.67%, even if improving 1% at this area inductivity is all very large raising, in addition be the raising of more than 3%, therefore, luckyly in the present invention highly lure that to be the inductivity of No. 31 far above existing induction be, the haploid-induction of 10.40%, the Stock6/M278-8-9-1-6 that lures as Ji is high that to be the haploid-induction of No. 3 be is 8.58%; (2) Ji used in the inventive method is high to lure and is No. 31 and has more significantly seed Navajo genetic marker, i.e. purple aleurone layer and purple embryo labels than other induction frenulums.With Ji high lure be No. 31 give other corn material pollination after, normal dliploid shows as purple aleurone layer and purple plumule point, the leaf sheath purple of plant, and the parthenocarpy seed aleurone layer purple that induction produces, plumule point is colourless, and plant leaf sheath is green, can confirm with this mark, seed mark property is obvious, and monoploid seed is easy to differentiate, other comprehensive agronomy proterties is good; (3), lucky high the luring of induction system used in the inventive method be No. 31 proper heights, and flowering time is long and pollen amount is large, can carry out spontaneous pollination induction monoploid, remove artificial pollination from, not only save labour, and be applicable to the operation carrying out large-scale groups; (4), the inventive method breeding efficiency is high, and luring because Ji used in the inventive method is high is that the haploid-induction of No. 31 is high, and the progeny population obtained so is under the same conditions large, and the probability selecting Elite inbred increases greatly, improves breeding efficiency; (5), the inventive method breeding cost is low, and because haploid-induction is high, and utilize compared with other haploid inducing lines, required unit breeding population is relatively little, reduces breeding cost; (6), the purity of inbred line that is bred as of the inventive method is high, and the purity of the crossbreed utilizing it to prepare is also high, is conducive to giving full play to heterotic effect; (7), the inventive method procedure of breeding is simple, saves to selfing purge processes more than at least 4 generations of filial generation in conventional method, directly pass through obtain doubling monoploids, breeding time also shortens greatly.
Embodiment
Embodiment 1: utilize lucky high luring to be No. 31 hybridized induction monoploid selecting and breeding corn inbred lines
Carry out as follows:
(1), 2010 years, utilize lucky high luring to be No. 31 and to make male parent and external crossbreed T628394 that Comprehensive Traits excellent high to output and pollinate in Jilin Academy of Agricultural Science, fruit ear 248 fringe on results maternal plant, seed 42326 is obtained after threshing, according to seed mark property, namely top aleurone layer and plumule point are the seed of purple, are the dliploid seeds of normal hybridisation; And top aleurone layer is purple, plumule point is colourless, and embryo face is less, triangular in shape, and depression marks more deeply, be then " accurate monoploid " seed of the T628394 parthenocarpy be induced out; " accurate monoploid " seed 6023 is obtained altogether, the haploid-induction=accurate monoploid seed/hybridization grain number × 100%=6023/42326 × 100%=14.23% of seed according to above-mentioned mark.
(2), the winter in 2010, " accurate monoploid " the seed monoseeding will filtered out in base, Hainan, academy of agricultural sciences of Jilin Province, emerging, (haploid breeding is to gametophytic selection in 5847 strains, each gamete has 1 cover chromosome set, each gene exists in singular form, all necessary challenge of environment of accepting and the pressure of natural selection, and therefore much monoploid can not grow and deteriorate to embryoless seeds; Some monoploid seed can not germinate because of depauperation; Even if or germinate because bud gesture is too weak and dead).Shooting stage confirms haplobiont further according to seedling growth, plant height, length of blade, leaf sheath (sheet) color and Leaf insertion angle etc.Haploid plantlets growing way is slow, plant height is low, plant is thin and weak, blade is short and compared with upper punch, leaf look shallow; Dliploid seedling growth is strong, plant is high and blade is loose, leaf sheath (sheet) purple, confirms haplobiont 5663 strain, dliploid (hybrid strain) 184 strain.Because hybrid strain is normal dliploid, its germination vigor and growth potential are all comparatively strong, and by the precision management in meticulous program request and field, can grow up to normal plant, therefore hybrid strain number also just represents pseudo-monoploid kernal number.So haploid-induction=accurate monoploid kernal number-hybrid strain number/hybridization kernal number × 100%=(6023-184)/42326 × 100%=13.80%.Through field Natural double, obtain plant 455 strain of not only normally reeling off raw silk from cocoons but also normally blooming, obtain 389, selfing fruit ear by selfing, single fringe is solid be 1 to 154 not etc.Other 66 strains due to the florescence inharmonious, there is no selfing fruit ear.
(3), spring in 2011, in Jilin Academy of Agricultural Science, the self-fertility fruit ear that the winter in 2010 obtains in Hainan is planted into head progeny row, and carry out selfing, neat and consistent is selected in self progeny, the new corn inbred line 24 that Comprehensive Traits does very well, name lucky DH628394-1 respectively, lucky DH628394-2, lucky DH628394-3, lucky DH628394-4, DH628394-5, lucky DH628394-6, lucky DH628394-7, lucky DH628394-8, DH628394-9, lucky DH628394-10, lucky DH628394-11, lucky DH628394-12, DH628394-13, lucky DH628394-14, lucky DH628394-15, lucky DH628394-16, DH628394-17, lucky DH628394-18, lucky DH628394-19, lucky DH628394-20, DH628394-21, lucky DH628394-22, lucky DH628394-23, lucky DH628394-24.
The above results illustrates that the inventive method haploid-induction is high, and seed Navajo genetic marker is obvious, and Screening and Identification monoploid seed ratio is easier to; The procedure of breeding is short, and breeding speed is fast, breeding efficiency is high, and breeding cost is low.
Embodiment 2: utilize lucky high luring to be No. 31 hybridized induction monoploid selecting and breeding corn inbred lines
Carry out as follows:
(1), 2007, with lucky 853, lucky 854, prosperous 7-2,4 287, capital 24,773-2, yellow C, yellow early four, 4 444, colony's (being called for short " yellow early four colonies ") of the wise man 461 artificial mixed pollination synthesis of totally 10 inbred lines with yellow early four blood relationships.2010, material based on " yellow early four colonies ", with Ji high lure be No. 31 for male parent is to " early yellow four colonies " pollination, fruit ear 315 fringe on results maternal plant, to set seeds altogether grain 53897, by top aleurone layer be purple, plumule point without purple, and embryo face is less, triangular in shape, the depression mark property such as darker is differentiated, acquisition " accurate monoploid " seed 7589.
(2), the spring in 2011, " accurate monoploid " the seed monoseeding will filtered out in Jilin Academy of Agricultural Science, 7431 strains of emerging, the shooting stage confirms haplobiont 6983 strain further according to seedling growth, plant height, length of blade, leaf color etc.; Plant growing way is strong, plant is tall and big, blade is loose, the hybrid plant of blade purple 247 strain.Because hybrid strain is normal diploid, its germination vigor and growth potential are all comparatively strong, and by the precision management in meticulous program request and field, can grow up to normal plant, therefore hybrid strain number also just represents pseudo-monoploid kernal number.So the haploid-induction of this test=accurate monoploid kernal number-hybrid strain number/hybridization kernal number × 100%=(7589-247)/53897 × 100%=13.62%.Through field Natural double, obtain plant 636 strain of not only normally reeling off raw silk from cocoons but also normally blooming, obtain 463, selfing fruit ear by selfing, single fringe is solid be 1 to 202 not etc.Other 173 strains, due to the reason such as the florescence is inharmonious, there is no selfing fruit ear.
(3), spring in 2012, in Jilin Academy of Agricultural Science, the self-fertility fruit ear that 2011 obtain is planted into head progeny row, field carefully manages and carries out self-fertility, neat and consistent is selected in self progeny, the new corn inbred line 20 that Comprehensive Traits does very well, name lucky DH Huang 1 respectively, lucky DH Huang 2, lucky DH Huang 3, lucky DH Huang 4, lucky DH Huang 5, lucky DH Huang 6, lucky DH Huang 7, lucky DH Huang 8, lucky DH Huang 9, the yellow 10 lucky DH Huangs 11 of lucky DH, lucky DH Huang 12, lucky DH Huang 13, lucky DH Huang 14, the yellow 15 lucky DH Huangs 16 of lucky DH, lucky DH Huang 17, lucky DH Huang 18, lucky DH Huang 19, lucky DH Huang 20.
The above results illustrates that the inventive method haploid-induction is high, and seed Navajo genetic marker is obvious, and Screening and Identification monoploid seed ratio is easier to; The procedure of breeding is short, and breeding speed is fast, breeding efficiency is high, and breeding cost is low.
Bibliography
1、CoeEH.AmerMat,1959,93:381－382
2、Lashermes,P,MBeckert.TheorApplGenet,1988,76:405－410
3、ShatskayaOA,ERZabirova,VSScherbaketal.MaizeGenetCoopNewsLett,1994,68:51
4、ChalykST.Euphytica,1994,79:13～18
5、ChaseSS.Productionofhomozygousdiploidsofmaizefrommonoploids[J].Agron.J.,1952(44):263-267
6、GayenP,JKMaden,RKumar,etal.Chromosomedoublinginhaploidsthroughcolchicines[J].MaizeGenet.Coop.NewsLett.,1994(68)64-65
7、ChalykST,VVOstrovskyandVVOstrovakii.Comparisonofhaploidmaizeplantswithidenticalnotypes[J].J.Genet.Breed,1993,(47):77-80
8, Wei person of outstanding talent etc., Maize Sciences, 2001,9 (3): 12-13
9, Liu Zhizeng, Song Tongming, Acta Agronomica Sinica, 2000,26 (5): 570-574
10, Liu Zhizeng, Song Tongming etc., China Agricultural University's journal, 2000,5 (3): 51-57
11, Chen Shaojiang, Song Tongming, Acta Agronomica Sinica, 2003,29 (4): 587-590.
Claims (6)
1. utilize the method for hybridized induction monoploid selecting and breeding corn inbred line, it is characterized in that comprising the steps:
(1), with high the luring of Ji be No. 31 and make male parent to meeting the basic population of breeding objective or crossbreed pollination, gather in the crops maternal fruit ear; Therefrom screen aleurone layer top purple, plumule point is without purple, and embryo face is less, triangular in shape, the seed of the comparatively dark mark of depression;
(2), to step (1) obtain material and carry out artificial doubling or Natural double, the shooting stage removes leaf sheath and blade is the plant of purple; Selfing is carried out to the plant that can normally bloom after doubling, according to individual plant results self-fertility seed;
(3), plant step (2) gained seed, select characters of progenies performance neat and consistent, do not have separative head progeny row, be new corn inbred line.
2. in accordance with the method for claim 1, it is characterized in that described Natural double refers under field conditions (factors), monoploid seed or the intracellular chromosome doubling of seedling plants become normal dliploid.
3. in accordance with the method for claim 1, it is characterized in that described artificial doubling refers to utilize colchicine solution process monoploid seed or seedling, make its endocellular chromosome double to become normal dliploid.
4. in accordance with the method for claim 1, it is characterized in that described basic population refers to by multiple inbred line or kind after mixed pollination, the maize population of formation.
5. in accordance with the method for claim 1, it is characterized in that described crossbreed refers to single cross hybrid, triple hybrid or double cross hybrid.
6. utilize the corn inbred line produced in the method described in claim 1 to cultivate the method for corn hybrid seed, it is characterized in that with the corn inbred line cultivated in claim 1 for one of parent, with other hybridizations between selfed lines, be bred as new crossbreed.
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| CN105660381A (en) * | 2016-02-25 | 2016-06-15 | 安徽省农业科学院烟草研究所 | Method for producing large-scale induction pollen sources in maize haploid breeding |
| CN108377899B (en) * | 2018-02-13 | 2021-11-05 | 吉林省农业科学院 | Breeding method for auxiliary selection of corn hybridization induced haploid by yellow-green seedling marker |
| CN108243947B (en) * | 2018-02-13 | 2021-08-24 | 吉林省农业科学院 | Breeding method for breeding corn early doubled haploid by yellow-green seedling marker |
| CN108834885A (en) * | 2018-05-25 | 2018-11-20 | 吉林省农业科学院 | A kind of corn inbred lines method |
| CN109757365A (en) * | 2019-03-19 | 2019-05-17 | 广西农业职业技术学院 | A kind of method of quick breeding silage corn self-mating system |
| CN113455378A (en) * | 2021-08-04 | 2021-10-01 | 北大荒垦丰种业股份有限公司 | Breeding method of corn haploid induction line and application thereof |
| CN115104525A (en) * | 2022-06-28 | 2022-09-27 | 北京市农林科学院 | Breeding method combining haploid breeding with conventional breeding and induced selection of corn and application of breeding method |
| CN115316263A (en) * | 2022-08-03 | 2022-11-11 | 吉林省农业科学院 | Cultivation method of isolation material Jiju No. 1 in corn test area |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843092A (en) * | 2006-05-18 | 2006-10-11 | 吉林省农业科学院 | Method for selecting and breeding corn new-bred through hybridized induction of unisexual seeding material |
| US7816585B1 (en) * | 2008-04-15 | 2010-10-19 | Pioneer Hi-Bred International, Inc. | Maize variety X6F624 |
| CN102334447A (en) * | 2010-07-23 | 2012-02-01 | 沈天民 | Cultivation method of maize inbred line |
| CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
-
2013
- 2013-03-07 CN CN201310072944.5A patent/CN104025998B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843092A (en) * | 2006-05-18 | 2006-10-11 | 吉林省农业科学院 | Method for selecting and breeding corn new-bred through hybridized induction of unisexual seeding material |
| US7816585B1 (en) * | 2008-04-15 | 2010-10-19 | Pioneer Hi-Bred International, Inc. | Maize variety X6F624 |
| CN102334447A (en) * | 2010-07-23 | 2012-02-01 | 沈天民 | Cultivation method of maize inbred line |
| CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
Non-Patent Citations (3)
| Title |
|---|
| New inducers of maternal haploids in maize;Rotarenco V.,et al.;《Maize Genetics Cooperation Newsletter》;20101231;第84卷;第1-7页 * |
| 玉米杂交诱导单倍生殖（单倍体）选育自交系技术规范（暂行版）;才卓等;《玉米科学》;20091231;第17卷(第5期);摘要 * |
| 玉米高频率单倍体生殖诱导系吉高诱系3号的选育;才卓等;《玉米科学》;20071231;第15卷(第1期);第2页第1.2-1.4节 * |
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