CN105660381A - Method for producing large-scale induction pollen sources in maize haploid breeding - Google Patents

Abstract

The invention relates to a method for producing large-scale induction pollen sources in maize haploid breeding. Hybridized inducing haploid combinations S04, S05 and S06 are obtained through hybridizing a Stock6 improved line with a high inducer I, or hybridizing the Stock6 improved line with MT041 or hybridizing the high inducer I with the MT041. During the maize haploid breeding, the S04, S05 and S06 serve as male parents and are hybridized with female parent materials of expected haploids, fruit ears of female parents are completely pollinated, haploid hybridized induction is carried out, and harvested hybrid seeds are subjected to screening, identifying and selecting, so that maize haploids are obtained. According to the method, inducing-line hybridized combinations represent heterosis of relatively high strength, and growth potential, comprehensive resistance and pollen quantity of the three inducing-line hybridized combinations are all obviously superior to those of 3 inducing lines; the shortages of the existing haploid inducing lines that the germinating potential and growth potential are weak, lodging and leaf spot infecting are easy, the volume of loose powder is low, and the like are overcome, and the inducing-line hybridized combinations with relatively stable haploid induction capability are screened out through haploid inducing effect tests and serve as the large-scale induction pollen sources in the haploid breeding.

Description

The production method of extensive induction pollen source in a kind of corn monoploid breeding

Technical field

The present invention relates to Crop Genetic Breeding, specifically a kind of is the extensive production method inducing pollen source in corn monoploid breeding.

Background technology

Corn be human use's hybrid vigour the earliest, the most extensively, the most significant crop of effect. Within 2014, China's corn seeding area reaches 5.56 hundred million mu, and ultimate production reaches 4,313 hundred million jin, according to statistic data disclosed in department of crop cultivation of the Ministry of Agriculture, has currently become the big crop of China's cultivated area first. In the later stage seventies 20th century, China achieves the universal of Corn Single-Cross Stock, improves corn yield significantly. And then corn breeding man starts to utilize on a large scale the traditional breeding method means seed selection self-mating systems such as seconed cycle method, to improve output and the disease resistance of corn hybrid seed, but the self-mating system of isozygotying that the method obtains high-combining ability needs the self cross and selection through 6-10 generation usually.

In recent years, monoploid breeding technique has become the big core technology of the modern corn breeding three compared favourably with molecular mark technology, transgenic technology. The breeding time limit can be significantly shortened in monoploid breeding, generally only needs generation selfing can obtain self-mating system of isozygotying. 1966, Sarkar etc. report Randolph and Stadler in the nineteen twenty-nine haploid event of Late Cambrian corn, within 1949, Chase proposes the method that live body induction is induction, nineteen fifty, the American Genetic man doctor Coe corn high frequency that obtains tool white endosperm and purple aleurone layer brought out haploid material, the monoploid that male parent induction normal plant can produce about 1-2% is done with this material, within 1956, Coe is on USA Magazine MaizeGeneticsNewsletter, and this can be induced the self-mating system called after Stock6 of Haploid production. Owing to not having in order to differentiate haploid genetic marker when Stock6 is found at first, in order to morphologically differentiate monoploid seed grain and hybridization diploid seed grain, Coe etc. are successively by ACR-nj gene and ABPI channel genes Stock6, the former controls seed grain aleurone layer and the formation of plumule point pigment, the latter controls the formation of adventive root, leaf sheath and stem stalk pigment, and Stock6 is just provided with seed grain and the two dominant inheritance mark of plant afterwards.Genetic marker method has become the qualification topmost method of monoploid.

Summary of the invention

It is difficult in satisfied current Hybrid breeding in commercial system to induce requirement on a large scale, the present invention is directed to corn monoploid characters of breeding for solving existing corn haploid induction line, it is provided that the production method of extensive induction pollen source in a kind of corn monoploid breeding. The method is the inductivity height by filtering out and stable induction is cross combination, comes as induction paternal pollen source extensive in corn monoploid breeding.

The technical solution used in the present invention is as follows:

The production method of extensive induction pollen source in corn monoploid breeding, its feature is,

Lure No. 1 hybridization with Stock6 Improved lines and height, or hybridize with Stock6 Improved lines and MT041, or to lure No. 1 to hybridize with MT041 with height; Results hybridized induction monoploid combination S04, S05, S06.

A kind of corn method for breeding haploidy, its feature is, comprises the steps:

S1, inducement crossbreeding combine

Lure No. 1 hybridization with Stock6 Improved lines and height, or hybridize with Stock6 Improved lines and MT041, or to lure No. 1 to hybridize with MT041 with height; Results hybridized induction monoploid combination S04, S05, S06;

S2, corn monoploid obtain

Using S04, S05, S06 as male parent, obtaining haploid female parent material with expectation and hybridize, maternal fruit ear is all pollinated hybridized induction monoploid, chooses through Screening and Identification and obtain corn monoploid from the cenospecies of results.

Further, described Screening and Identification refers to:

(1) children's seedling screening:

By the plant color identification monoploid of R-nj Gene Handling: purple plant is hybrid strain, green plant is monoploid;

(2) seed harvest phase screening: identify monoploid by genetic marker identification method, based on R-nj genetic marker system, taking the Zi Ding of seed grain with purple embryo as dominant marker; Being identified by grain by the cenospecies of results, the seed grain of the white embryo in purple top is monoploid, and the seed grain of the purple embryo in purple top is the diploid of normal hybridisation, and Bai Dingbai embryo is the diploid seed by pollen contamination.

Compared with the prior art, the useful effect of the present invention is embodied in:

The present invention's induction is the hybrid vigour that cross combination shows higher-strength, and 3 inductions are that cross combination is all obviously better than 3 induction systems from growth potential (strain height), comprehensive resistance, pollen amount; The germinating energy, the growth potential that overcome the existence of existing haploid inducing line are weak, easily lodging and sense leaf spot, loose powder amount waits not enough less, by haploid induction measure of merit, filtering out the induction with comparatively stable haploid induction ability is cross combination, induces pollen source as in extensive monoploid breeding.

Accompanying drawing explanation

Fig. 1 is the present invention 3 induction system and 3 inductions is cross combination field plant type figure.

Embodiment

By embodiment, technical solution of the present invention is further explained explanation below in conjunction with accompanying drawing.

In following examples, S01 is Stock6 Improved lines, draws from the Chinese Academy of Agricultural Sciences; S02 is that height lures No. 1, draws from China Agricultural University; S03 is MT041, draws from the Chinese Academy of Agricultural Sciences; 3 above-mentioned induction systems all mark with the two dominant inheritance described in background technology.

To be induced material one be 2 kinds of maize genotypes cross-fertilize seed: M01 for stepping on sea 618, M02 is enlightening card 517. It is induced the F1 generation that material two is the free self-mating system assembly of 2 in same hybrid vigour group: M03 is 7022 × 4006, M04 be 4015 × 4006, M05 be 1006 × 1007, M06 is 1011 × 4004.Material one and material two are existing well known materials.

1, inducement crossbreeding combination

Lure No. 1 hybridization with Stock6 Improved lines and height, or hybridize with Stock6 Improved lines and MT041, or to lure No. 1 to hybridize with MT041 with height; Results hybridized induction monoploid combination S04, S05, S06.

See Fig. 1, table 1, above-mentioned induction is the hybrid vigour that cross combination shows higher-strength, and 3 inductions are that cross combination is all obviously better than 3 induction systems from growth potential (strain height), comprehensive resistance, pollen amount.

Table 13 induction system and 3 inductions are cross combination principal feature

2, hybridized induction monoploid

Doing male parent with S01～S06, do female parent with M01～M06, hybridized induction monoploid, concrete grammar is:

12 row respectively planted by M01 and M02 material, and 2 row respectively planted by M03～M06 material, and male parent material plants 2 row respectively, father and mother this by interval sowing come florescence alternation to guarantee the florescence meet. The long 5m of cell row, line-spacing 0.6m, spacing in the rows 0.25m.

A, the 6-7 month in 2014 do male parent at corn research centre, Anhui academy of agricultural sciences base, Fengyang S01～S06, do female parent with M01～M02, and maternal fruit ear is all pollinated hybridized induction monoploid, and from the cenospecies of results, monoploid is selected in qualification. 12 row respectively planted by M01 and M02 material, and male parent material plants 2 row respectively, father and mother this by interval sowing come florescence alternation to guarantee the florescence meet. The long 5m of cell row, line-spacing 0.6m, spacing in the rows 0.25m.

B, January in December, 2014 to 2015 year do male parent in S04～S06 combination that numerous base, the happy southeast, corn research centre, Anhui academy of agricultural sciences inductivity height, comprehensive proterties are excellent, female parent is done with M03～M06, maternal fruit ear is all pollinated hybridized induction monoploid, and from the cenospecies of results, monoploid is selected in qualification.

Haploid authentication method is:

(1) children's seedling screening:

By the plant color identification monoploid of R-nj Gene Handling: purple plant is hybrid strain, green plant is monoploid;

(2) seed harvest phase screening: identify monoploid by genetic marker identification method, based on R-nj genetic marker system, taking the Zi Ding of seed grain with purple embryo as dominant marker; Being identified by grain by the cenospecies of results, the seed grain of the white embryo in purple top is monoploid, and the seed grain of the purple embryo in purple top is the diploid of normal hybridisation, and Bai Dingbai embryo is the diploid seed by pollen contamination; And it is a small amount of without embryo seed.

See table 2, S04 and S06 haploid-induction in M01 and M02 is all not less than the inductivity that its parent induces system, illustrates that haploid inducing line cross combination S04 and S06 has super close advantage on haploid-induction.

Table 23 induction is cross combination and the haploid-induction of 3 induction systems

Haploid-induction=monoploid strain number/total grain number × 100%.

See table 3, S04 and S06 all keeps higher haploid-induction in M03～M06, and inductivity is up to 5.87%, and minimum is 3.62%, illustrates that haploid inducing line cross combination S04 and S06 haploid induction ability are more stable.

Table, 32 inductions are the haploid-induction of cross combination

The present invention utilizes the hybrid vigour that haploid inducing line cross combination shows, the germinating energy, the growth potential that overcome the existence of existing haploid inducing line are weak, easily lodging and sense leaf spot, loose powder amount waits not enough less, by haploid induction measure of merit, filtering out the induction with comparatively stable haploid induction ability is cross combination, induces pollen source as in extensive monoploid breeding.

The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment of doing, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (3)

1. the extensive production method inducing pollen source in a corn monoploid breeding, it is characterised in that,

Lure No. 1 hybridization with Stock6 Improved lines and height, or hybridize with Stock6 Improved lines and MT041, or lure No. 1 to hybridize with MT041 with height; Results obtain hybridized induction monoploid combination S04, S05, S06.

2. a corn method for breeding haploidy, it is characterised in that, comprise the steps:

S1, inducement crossbreeding combine

Lure No. 1 hybridization with Stock6 Improved lines and height, or hybridize with Stock6 Improved lines and MT041, or to lure No. 1 to hybridize with MT041 with height; Results hybridized induction monoploid combination S04, S05, S06.

S2, corn monoploid obtain

Using S04, S05, S06 as male parent, obtaining haploid female parent material with expectation and hybridize, maternal fruit ear is all pollinated hybridized induction monoploid, chooses through Screening and Identification and obtain corn monoploid from the cenospecies of results.

3. a kind of corn method for breeding haploidy according to claim 2, it is characterised in that, described Screening and Identification refers to:

(1) children's seedling screening:

By the plant color identification monoploid of R-nj Gene Handling: purple plant is hybrid strain, green plant is monoploid;

(2) seed harvest phase screening: identify monoploid by genetic marker identification method, based on R-nj genetic marker system, taking the Zi Ding of seed grain with purple embryo as dominant marker; Being identified by grain by the cenospecies of results, the seed grain of the white embryo in purple top is monoploid, and the seed grain of the purple embryo in purple top is the diploid of normal hybridisation, and Bai Dingbai embryo is the diploid seed by pollen contamination.