CN102334447A - Cultivation method of maize inbred line - Google Patents
Cultivation method of maize inbred line Download PDFInfo
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- CN102334447A CN102334447A CN2010102362009A CN201010236200A CN102334447A CN 102334447 A CN102334447 A CN 102334447A CN 2010102362009 A CN2010102362009 A CN 2010102362009A CN 201010236200 A CN201010236200 A CN 201010236200A CN 102334447 A CN102334447 A CN 102334447A
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Abstract
The invention discloses a cultivation method of a maize inbred line, comprising the following steps of: (1) selective breeding of an induced line ''Lanyu induced No.1'' with high induction frequency from inductive materials of different sources; (2) induction method: taking the inductive materials as female parent and the ''Lanyu induced No.1'' as male parent according to the breeding objective to perform a test-cross, firstly selecting a female parent plant with normal growth to remove tassel, holding up pistil before wiredrawing, pollinating female parent by using pollen of ''Lanyu induced No.1'' when wiredrawing of filament finishes, quickly carrying out pollination and simultaneously taking off paper bags, holding up the paper bags after pollination, hanging with tags and fixing, performing ten panicles for each test-cross combination, harvesting and drying by sun according to test-cross combination; (3) selecting of monoploid grain; (4) identifying and treating of monoploid seedling; (5) enhancing field fertilizer and water management as well as cultivating and weeding; (6) early generation test-cross: inducing the duplex second generation inbred line into normal growth and development, recovering to normal growth of plant morphological characteristics, screening the inbred line by early generation test-cross to obtain a qualified maize inbred line. The invention can be used to cultivate a rapid and stable maize inbred line.
Description
Technical field
The present invention relates to the crop breeding method, particularly relate to a kind of breeding method of corn inbred line.
Background technology
At present; Along with rapid economy development; In quick growth, this just requires the corn scientific research for producing the various dissimilar corn varieties of cultivating more output height, quality better, disease-resistant, wide adaptability, strong stress resistance to the demand of corn for industry, livestock breeding, biological industry.Good corn variety is to be formed by the hybridization between selfed lines that good output is high, coordinate force is good, so the good inbred line of seed selection is exactly the most important thing problem of corn breeding.Conventional corn inbred line breeding method generally is " a comprehensive selection method ", and " second cycle line back-and-forth method " rises " flower pesticide (powder) cultivation " recent years; The former is long the time, can not select breakthrough inbred line of isozygotying, and latter's technical difficulty is complicated; Expensive; Experimental instrument and equipment is many, and investment is big, is difficult to carry out.The haploid breeding induction frequency of carrying out in the world is low, doubles difficulty, is difficult to extensive use in the breeding.The haploid frequency of corn abiogenesis is 0.05～0.1%; And states such as Russia, the U.S., Mexico, Germany, Italy in the last few years; Haploid method is induced in employing; Induction frequency is all 4～5%, and units such as China China Agricultural University adopt the induction frequency of this method to bring up to about 10%, and its main key technology is in the cultivation of induced material.
Summary of the invention
The breeding method that the purpose of this invention is to provide a kind of corn inbred line can be cultivated a kind of corn inbred line of fast and stable.
For realizing above-mentioned purpose, technical scheme of the present invention is:
A kind of breeding method of corn inbred line may further comprise the steps:
(1) material selects to adopt the method for phase mutual cross, repeated screening, and from the induced material of difference source, selecting inducing of high induction frequency is " Lan Yu lures No. one ";
(2) abductive approach: is maternal according to breeding objective with induced material, and " Lan Yu lures No. one " surveyed for male parent and joined, and at first selects maternal growth normal plant to take out tassel; Female fringe entangles firm with sheepskin paper bag or sulfuric ester bag before reeling off raw silk from cocoons, in order to avoid receive outside pollen, treat that filigree is taken out together basically after; Give maternal pollination with the pollen of " Lan Yu lures No. one "; Action will be taken off paper bag soon simultaneously during pollination, puts paper bag after the pollination, and it is not good to hang up behind the label; Each is surveyed combo and closes (comprising female parent and male parent) and make ten fringes, closes results and dries by surveying combo during results;
(3) the monoploid seed is selected: after the results induce fringe by the combination threshing, after the threshing monoploid seed is picked out;
(4) monoploid seedling identification and handling: the monoploid seedling is recognized, and it is carried out chromosome doubling handle and make it become dliploid;
(5) strengthen field rich water quality management and intertill and clean tillage;
(6) early the generation survey is joined: induce the second generation inbred line that doubles to get into normal growth and grow, and plant forms characteristic recovery normal growth, early generation survey pairing inbred line is screened, and obtains qualified corn inbred line.
Preferably, in the step according to the invention (2) induced material be 867,934,872,873,251 wherein a kind of.
Preferably, the monoploid seed has the endosperm top of purple when selecting in the step according to the invention (3), the plumule of white.
Preferably, the monoploid seedling leaves is few when recognizing in the step of the present invention (4), and blade is narrow, and is upright, indivedual plant leaf languedoc marbles, and plant is thin and weak, poor growth, the leaf look dark green, and cell is little.
Therefore compared with prior art, the present invention has following beneficial effect:
(1) method of the mutual cross of material selection employing phase, repeated screening in the step of the present invention (1); The haplotype frequency of inducing that selects reaches 15～23%; External same type of material has improved 4～5 times, and monoploid has the visible marking on female fringe, selects easy and convenient;
(2) in the step of the present invention (4) the monoploid chromosome doubling is become dliploid, so just can be applied to breeding, monoploid chromosome doubling method has two kinds: a kind of is that nature doubles; Another kind is that chemical agent is handled; The monoploid seedling of adopting " Lan Yu lures No. one " induced material to induce, owing to influenced by genotype, tassel adds overtones band naturally and surpasses more than 33%; Fully can be without colchicine or weed killer herbicide medicament processing method, guaranteeing has the pure lines of q.s to be applied in the middle of the actual breeding;
(3) owing to a little less than the monoploid generation growth potential that induces,, can guarantee that the monoploid seedling has a good growth and development environment, select effect to improve so step of the present invention (4) is strengthened field rich water quality management and intertill and clean tillage;
(4) the present invention can make the inbred line fast and stable, has solved in the past at the bottom of the corn induction frequency, doubles difficult key technical problem, and shorten and cultivate 4～5 years inbred line cycles, be the shortcut of quickly culturing homozygous inbred line;
(5) inbred line that selects of the present invention all is the colony of isozygotying, and is the ideal mapping material that makes up genetic linkage maps, can cross and repeat mapping and sampling.
Embodiment
To combine specific embodiment to specify the present invention below, be used for explaining the present invention in this illustrative examples of the present invention and explanation, but not as to qualification of the present invention.
Cultivate corn inbred line according to following steps:
(1) material selects to adopt the method for phase mutual cross, repeated screening, and from the induced material of difference source, selecting inducing of high induction frequency is " Lan Yu lures No. one ";
(2) abductive approach: is maternal according to breeding objective with induced material, and " Lan Yu lures No. one " surveyed for male parent and joined, and at first selects maternal growth normal plant to take out tassel; Female fringe entangles firm with sheepskin paper bag or sulfuric ester bag before reeling off raw silk from cocoons, in order to avoid receive outside pollen, treat that filigree is taken out together basically after; Give maternal pollination with the pollen of " Lan Yu lures No. one "; Action will be taken off paper bag soon simultaneously during pollination, puts paper bag after the pollination, and it is not good to hang up behind the label; Each is surveyed combo and closes (comprising female parent and male parent) and make ten fringes, closes results and dries by surveying combo during results;
(3) the monoploid seed is selected: after the results induce fringe by the combination threshing, after the threshing monoploid seed is picked out;
(4) monoploid seedling identification and handling: the monoploid seedling is recognized, and it is carried out chromosome doubling handle and make it become dliploid;
(5) strengthen field rich water quality management and intertill and clean tillage;
(6) early the generation survey is joined: induce the second generation inbred line that doubles to get into normal growth and grow, and plant forms characteristic recovery normal growth, early generation survey pairing inbred line is screened, and obtains qualified corn inbred line.
During practical operation, in the above-mentioned steps (2) induced material be 867,934,872,873,251 wherein a kind of; The monoploid seed has the endosperm top of purple when selecting in the above-mentioned steps (3), the plumule of white; The monoploid seedling leaves is few when recognizing in the above-mentioned steps (4), and blade is narrow, and is upright, indivedual plant leaf languedoc marbles, and plant is thin and weak, poor growth, the leaf look dark green, and cell is little.
More than the technical scheme that the embodiment of the invention provided has been carried out detailed introduction; Used concrete example among this paper the principle and the embodiment of the embodiment of the invention are set forth, the explanation of above embodiment only is applicable to the principle that helps to understand the embodiment of the invention; Simultaneously, for one of ordinary skill in the art, according to the embodiment of the invention, the part that on embodiment and range of application, all can change, in sum, this description should not be construed as limitation of the present invention.
Claims (4)
1. the breeding method of a corn inbred line is characterized in that may further comprise the steps:
(1) material selects to adopt the method for phase mutual cross, repeated screening, and from the induced material of difference source, selecting inducing of high induction frequency is " Lan Yu lures No. one ";
(2) abductive approach: is maternal according to breeding objective with induced material, and " Lan Yu lures No. one " surveyed for male parent and joined, and at first selects maternal growth normal plant to take out tassel; Female fringe entangles firm with sheepskin paper bag or sulfuric ester bag before reeling off raw silk from cocoons, in order to avoid receive outside pollen, treat that filigree is taken out together basically after; Give maternal pollination with the pollen of " Lan Yu lures No. one "; Action will be taken off paper bag soon simultaneously during pollination, puts paper bag after the pollination, and it is not good to hang up behind the label; Each is surveyed combo and closes (comprising female parent and male parent) and make ten fringes, closes results and dries by surveying combo during results;
(3) the monoploid seed is selected: after the results induce fringe by the combination threshing, after the threshing monoploid seed is picked out;
(4) monoploid seedling identification and handling: the monoploid seedling is recognized, and it is carried out chromosome doubling handle and make it become dliploid;
(5) strengthen field rich water quality management and intertill and clean tillage;
(6) early the generation survey is joined: induce the second generation inbred line that doubles to get into normal growth and grow, and plant forms characteristic recovery normal growth, early generation survey pairing inbred line is screened, and obtains qualified corn inbred line.
2. the breeding method of corn inbred line according to claim 1 is characterized in that: in the said step (2) induced material be 867,934,872,873,251 wherein a kind of.
3. the breeding method of corn inbred line according to claim 1, it is characterized in that: the monoploid seed has the endosperm top of purple when selecting in the said step (3), the plumule of white.
4. the breeding method of corn inbred line according to claim 1 is characterized in that: in the said step (4) during identification the monoploid seedling leaves few, blade is narrow, and is upright, indivedual plant leaf languedoc marbles, plant is thin and weak, poor growth, the leaf look dark green, cell is little.
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| CN2010102362009A CN102334447A (en) | 2010-07-23 | 2010-07-23 | Cultivation method of maize inbred line |
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| CN2010102362009A CN102334447A (en) | 2010-07-23 | 2010-07-23 | Cultivation method of maize inbred line |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103109734A (en) * | 2013-01-30 | 2013-05-22 | 南京市蔬菜科学研究所 | Breeding method for purple waxy corn inbred line |
| CN103461108A (en) * | 2013-09-17 | 2013-12-25 | 山东省农业科学院玉米研究所 | Simple pollination method used in corn breeding |
| CN103999769A (en) * | 2014-06-06 | 2014-08-27 | 山东省农业科学院玉米研究所 | Corn haploid inducing method |
| CN104025998A (en) * | 2013-03-07 | 2014-09-10 | 吉林省农业科学院 | Method for breeding corn inbred line by using hybridizing induction of haploids |
| CN106688876A (en) * | 2017-01-12 | 2017-05-24 | 山东省农业科学院玉米研究所 | Combining and matching method of maize |
| CN109122292A (en) * | 2018-08-25 | 2019-01-04 | 吉林农业科技学院 | A kind of breeding method of waxy corn DH system |
| CN111771592A (en) * | 2020-07-14 | 2020-10-16 | 广西壮族自治区农业科学院 | Method for improving fruit setting rate of exocarpium citri rubrum |
| CN114246081A (en) * | 2020-03-10 | 2022-03-29 | 华中农业大学 | Haploid inducer and application method thereof |
| CN115633635A (en) * | 2022-10-09 | 2023-01-24 | 武汉市农业科学院 | Method for creating sweet corn DH line based on haploid breeding technology |
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| US20070107100A1 (en) * | 2007-01-31 | 2007-05-10 | Pioneer Hi-Bred International, Inc. | Maize Hybrid Variety 33F12 |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103109734B (en) * | 2013-01-30 | 2014-03-19 | 南京市蔬菜科学研究所 | Breeding method for purple waxy corn inbred line |
| CN103109734A (en) * | 2013-01-30 | 2013-05-22 | 南京市蔬菜科学研究所 | Breeding method for purple waxy corn inbred line |
| CN104025998B (en) * | 2013-03-07 | 2016-04-20 | 吉林省农业科学院 | Utilize the method for hybridized induction monoploid selecting and breeding corn inbred line |
| CN104025998A (en) * | 2013-03-07 | 2014-09-10 | 吉林省农业科学院 | Method for breeding corn inbred line by using hybridizing induction of haploids |
| CN103461108A (en) * | 2013-09-17 | 2013-12-25 | 山东省农业科学院玉米研究所 | Simple pollination method used in corn breeding |
| CN103999769A (en) * | 2014-06-06 | 2014-08-27 | 山东省农业科学院玉米研究所 | Corn haploid inducing method |
| WO2015184574A1 (en) * | 2014-06-06 | 2015-12-10 | 山东省农业科学院玉米研究所 | Method for inducing corn haploid |
| CN103999769B (en) * | 2014-06-06 | 2016-06-01 | 山东省农业科学院玉米研究所 | A kind of corn haploid induction method |
| CN106688876A (en) * | 2017-01-12 | 2017-05-24 | 山东省农业科学院玉米研究所 | Combining and matching method of maize |
| CN109122292A (en) * | 2018-08-25 | 2019-01-04 | 吉林农业科技学院 | A kind of breeding method of waxy corn DH system |
| CN114246081A (en) * | 2020-03-10 | 2022-03-29 | 华中农业大学 | Haploid inducer and application method thereof |
| CN111771592A (en) * | 2020-07-14 | 2020-10-16 | 广西壮族自治区农业科学院 | Method for improving fruit setting rate of exocarpium citri rubrum |
| CN115633635A (en) * | 2022-10-09 | 2023-01-24 | 武汉市农业科学院 | Method for creating sweet corn DH line based on haploid breeding technology |
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Application publication date: 20120201 |