CN109122292A - A kind of breeding method of waxy corn DH system - Google Patents
A kind of breeding method of waxy corn DH system Download PDFInfo
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- CN109122292A CN109122292A CN201810976281.2A CN201810976281A CN109122292A CN 109122292 A CN109122292 A CN 109122292A CN 201810976281 A CN201810976281 A CN 201810976281A CN 109122292 A CN109122292 A CN 109122292A
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Abstract
The invention discloses a kind of breeding methods of waxy corn DH system, comprising the following steps: 1) selects excellent white (Huang) glutinous corn variety hybridization；2) it doubles to handle；3) selfing seed selection；4) stability is cultivated, i.e., obtains waxy corn DH system by continuing 1 generation of selfing.The present invention is by selecting excellent white (Huang) glutinous corn variety plant to induce Xi Ji section to lure 115 to be hybridized to obtain F1 generation seed with waxy corn monoploid tailored version male parent to be maternal, select waxy corn monoploid, it is doubled to handle and obtains waxy corn DH system by stringent screening and selfing, there is excellent self-fruitfulness in the parent culture of New Waxy Maize Hybrids, it can be used for glutinous corn breeding, be conducive to quickly breeding and go out the more excellent good quality and high output New Waxy Maize Hybrids with excellent Ear weight, reduce human and material resources consumption, has a vast market foreground.
Description
Technical field
The present invention relates to agricultural technology field, the breeding method of specifically a kind of waxy corn DH system.
Background technique
Waxy corn is also waxy corn, and compared with conventional corn, the nutrient composition content in waxy corn seed is higher than common
Corn has the effects that the flavor and taste of improving appetite and invigorating spleen, dehumidifying, diuresis, while waxy corn have characteristic.With people
Living standard be continuously improved, everybody is also constantly promoting the attention degree of ordinary meal structure, and due in waxy corn rely
Histidine content is higher than conventional corn, while the starch in waxy corn is entirely amylopectin, under the action of amylolytic enzyme
Digestibility receives the widespread love of people up to 85%.
Need to pursue the raising of corn yield in corn planting, and the Ear weight of corn yield and corn is with important
System, in order to meet the needs of people are growing to waxy corn, needs to cultivate the more excellent height with excellent Ear weight
Produce New Waxy Maize Hybrids.
Conventional corn breeding techniques need to consume a large amount of human and material resources, breeding year limit for length；And use haploid breeding
Technology can be quickly obtained the corn variety of high yield and high quality, and the dihaploid that doubling monoploids obtain is DH system, also referred to as DH
Group, pure lines group, can be directly used for breeding, greatly shortening breeding cycle.Currently, main in conventional corn haploid breeding
To make induction system using the Stock6 derived from the U.S. and hybridized induction Haploid production is carried out to female parent material, however it is in waxy corn
Inducing effect in monoploid cultivation is unsatisfactory, and doubling monoploids rate is low, and DH system robustness is poor.Therefore, it is necessary to design one kind
The breeding method of waxy corn DH system with good self-fruitfulness, to accelerate glutinous corn breeding process.
Summary of the invention
The object of the invention is to provide a kind of waxy corn DH system to solve above-mentioned problems of the prior art
Breeding method, be maternal lucky with waxy corn monoploid tailored version induction system by selecting excellent white (Huang) glutinous corn variety plant
Section lures 115 to be hybridized to obtain F1 generation and doubles to handle and obtain waxy corn DH system by stringent screening and selfing, in waxy corn training
There is excellent self-fruitfulness in educating, can be used for the breeding of waxy corn, be conducive to quickly breeding go out it is more excellent have it is excellent
The good quality and high output New Waxy Maize Hybrids of different Ear weight.
The purpose of the present invention can be achieved through the following technical solutions:
A kind of breeding method of waxy corn DH system, which is characterized in that it the following steps are included:
1) it selects excellent variety to hybridize: being that maternal and waxy corn monoploid tailored version induces with excellent white (Huang) glutinous corn variety plant
Xi Ji section lures 115 to be hybridized to obtain F1 generation, then by the seed sodium-chloride water solution of F1 generation in 28 DEG C of immersions 8h, Zhi Houzai
It is clean with aseptic water washing, filter out the seed A that embryo portion has purple to mark without purple label, aleurone；
2) it doubles to handle: by seed A obtained in step 1) with the seed-soaking liquid containing pendimethalin, Tween 80 and thiophene ketone at 30 DEG C
Processing 28h is doubled, is then cleaned up with deionized water, processing seed B must be doubled；
3) selfing seed selection: processing seed B will be doubled obtained in step 2 and conventionally plants and forms plant, is then selected
Female tassel all fertile single plants carry out bagging selfing, and it is more after fruit ear harvest to select and remain full seed, marshalling and tassel row number
Fruit ear carry out threshing and obtain single fringe seed C；
4) stability is cultivated: list fringe seed C obtained in step 3) being placed on filter paper, sprays distilled water and in illumination 3500-
5500Lx, vernalization is carried out under the conditions of 28 DEG C of constant temperature, conventionally planted to transplanting after 4 leaves to crop field after maize seedling is long
It plants, is then strictly screened and rejected hybrid strain, select fine individual plant to be selfed to get waxy corn DH system is arrived.
1. further, in step 1), the glutinous corn variety be change No. 1 glutinous glutinous 930, spring, Kennian No.1, osmanthus it is glutinous 519,
First glutinous No.1, Xi Xinghuang it is No. 6 glutinous, in glutinous No.1 or capital section glutinous one of 2000.
Further, in step 1), the concentration of the sodium-chloride water solution is 0.18mol/L；The sodium-chloride water solution
Additional amount be subject to and submerge seed just.
Further, in step 1), the time of the flushing is 15-25min.
Further, in step 2, the mass fraction of pendimethalin and Tween 80 is 0.012- in the seed-soaking liquid
0.018%, the mass fraction of thiophene ketone is 0.002-0.007%；The additional amount of the seed-soaking liquid is subject to submerges seed just.
Further, in step 2, the time of the cleaning is 15-25min.
2. further, in step 4), the stringent screening simultaneously rejects hybrid strain, fine individual plant is selected to be selfed.
Application of the breeding method of the waxy corn DH system in glutinous corn breeding.
In conclusion by adopting the above-described technical solution, the beneficial effects of the present invention are:
The present invention is maternal lucky with waxy corn monoploid tailored version induction system by selecting excellent white (Huang) glutinous corn variety plant
Section lures 115 to be hybridized to obtain F1 generation and doubles to handle and obtain waxy corn DH system by stringent screening and selfing, in waxy corn training
There is excellent self-fruitfulness in educating, can be used for the breeding of waxy corn, be conducive to quickly breeding go out it is more excellent have it is excellent
The good quality and high output New Waxy Maize Hybrids of different Ear weight reduce human and material resources consumption, have a vast market foreground.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.The present embodiment is based on the technical solution of the present invention
Implemented, the detailed implementation method and specific operation process are given, but protection scope of the present invention be not limited to it is following
Embodiment.
Embodiment 1
A kind of breeding method of waxy corn DH system, it specifically includes the following steps:
1) it selects excellent variety to hybridize: being maternal lucky with waxy corn monoploid tailored version induction system with excellent white glutinous corn variety plant
Section lures 115 to be hybridized to obtain F1 generation, and the seed sodium-chloride water solution of F1 generation is then used nothing in 28 DEG C of immersion 8h again later
Bacterium water is rinsed well, and the seed A that embryo portion has purple to mark without purple label, aleurone is filtered out；The glutinous corn variety is capital
Section glutinous 2000；The glutinous corn variety is commercial product；The concentration of the sodium-chloride water solution is 0.18mol/L；The chlorine
The additional amount for changing sodium water solution is subject to submerges seed just；The time of the flushing is 15min；
2) it doubles to handle: by seed A obtained in step 1) with the seed-soaking liquid containing pendimethalin, Tween 80 and thiophene ketone at 30 DEG C
Processing 28h is doubled, is then cleaned up with deionized water, processing seed B must be doubled；Pendimethalin and tween in the seed-soaking liquid
80 mass fraction is that the mass fraction of 0.012%, thiophene ketone is 0.002%；The additional amount of the seed-soaking liquid is with lucky submergence kind
Subject to son；The time of the cleaning is 15min；
3) selfing seed selection: processing seed B will be doubled obtained in step 2 and conventionally plants and forms plant, is then selected
Female tassel all fertile single plants carry out bagging selfing, and it is more after fruit ear harvest to select and remain full seed, marshalling and tassel row number
Fruit ear carry out threshing and obtain single fringe seed C；
4) stability is cultivated: list fringe seed C obtained in step 3) being placed on filter paper, sprays distilled water and in illumination
4500Lx, vernalization is carried out under the conditions of 28 DEG C of constant temperature, conventionally planted to transplanting after 4 leaves to crop field after maize seedling is long
It plants, is then strictly screened and rejected hybrid strain, select fine individual plant to be selfed to get waxy corn DH system is arrived；By selecting waxy corn
Kind plant is maternal to lure with waxy corn monoploid tailored version induction Xi Ji section 115 to be hybridized to obtain F1 generation and double to handle and pass through
It crosses stringent screening and selfing obtains waxy corn DH system, there is excellent self-fruitfulness, can be used for the breeding of waxy corn, be conducive to
Quickly breeding goes out the more excellent good quality and high output New Waxy Maize Hybrids with excellent Ear weight.
Embodiment 2
A kind of breeding method of waxy corn DH system, it specifically includes the following steps:
1) it selects excellent variety to hybridize: being maternal lucky with waxy corn monoploid tailored version induction system with excellent white glutinous corn variety plant
Section lures 115 to be hybridized to obtain F1 generation, and the seed sodium-chloride water solution of F1 generation is then used nothing in 28 DEG C of immersion 8h again later
Bacterium water is rinsed well, and the seed A that embryo portion has purple to mark without purple label, aleurone is filtered out；The glutinous corn variety is capital
Section glutinous 2000；The glutinous corn variety is commercial product；The concentration of the sodium-chloride water solution is 0.18mol/L；The chlorine
The additional amount for changing sodium water solution is subject to submerges seed just；The time of the flushing is 25min；
2) it doubles to handle: by seed A obtained in step 1) with the seed-soaking liquid containing pendimethalin, Tween 80 and thiophene ketone at 30 DEG C
Processing 28h is doubled, is then cleaned up with deionized water, processing seed B must be doubled；Pendimethalin and tween in the seed-soaking liquid
80 mass fraction is that the mass fraction of 0.018%, thiophene ketone is 0.007%；The additional amount of the seed-soaking liquid is with lucky submergence kind
Subject to son；The time of the cleaning is 25min；
3) selfing seed selection: processing seed B will be doubled obtained in step 2 and conventionally plants and forms plant, is then selected
Female tassel all fertile single plants carry out bagging selfing, and it is more after fruit ear harvest to select and remain full seed, marshalling and tassel row number
Fruit ear carry out threshing and obtain single fringe seed C；
4) stability is cultivated: list fringe seed C obtained in step 3) being placed on filter paper, sprays distilled water and in illumination
4500Lx, vernalization is carried out under the conditions of 28 DEG C of constant temperature, conventionally planted to transplanting after 4 leaves to crop field after maize seedling is long
It plants, is then strictly screened and rejected hybrid strain, select fine individual plant to be selfed to get waxy corn DH system is arrived；By selecting waxy corn
Kind plant is maternal to lure with waxy corn monoploid tailored version induction Xi Ji section 115 to be hybridized to obtain F1 generation and double to handle and pass through
It crosses stringent screening and selfing obtains waxy corn DH system, there is excellent self-fruitfulness, can be used for the breeding of waxy corn, be conducive to
Quickly breeding goes out the more excellent good quality and high output New Waxy Maize Hybrids with excellent Ear weight.
Embodiment 3
A kind of breeding method of waxy corn DH system, it specifically includes the following steps:
1) it selects excellent variety to hybridize: being maternal lucky with waxy corn monoploid tailored version induction system with excellent white glutinous corn variety plant
Section lures 115 to be hybridized to obtain F1 generation, and the seed sodium-chloride water solution of F1 generation is then used nothing in 28 DEG C of immersion 8h again later
Bacterium water is rinsed well, and the seed A that embryo portion has purple to mark without purple label, aleurone is filtered out；The glutinous corn variety is capital
Section glutinous 2000；The glutinous corn variety is commercial product；The concentration of the sodium-chloride water solution is 0.18mol/L；The chlorine
The additional amount for changing sodium water solution is subject to submerges seed just；The time of the flushing is 20min；
2) it doubles to handle: by seed A obtained in step 1) with the seed-soaking liquid containing pendimethalin, Tween 80 and thiophene ketone at 30 DEG C
Processing 28h is doubled, is then cleaned up with deionized water, processing seed B must be doubled；Pendimethalin and tween in the seed-soaking liquid
80 mass fraction is that the mass fraction of 0.015%, thiophene ketone is 0.0045%；The additional amount of the seed-soaking liquid is with lucky submergence kind
Subject to son；The time of the cleaning is 20min；
3) selfing seed selection: processing seed B will be doubled obtained in step 2 and conventionally plants and forms plant, is then selected
Female tassel all fertile single plants carry out bagging selfing, and it is more after fruit ear harvest to select and remain full seed, marshalling and tassel row number
Fruit ear carry out threshing and obtain single fringe seed C；
4) stability is cultivated: list fringe seed C obtained in step 3) being placed on filter paper, sprays distilled water and in illumination
4500Lx, vernalization is carried out under the conditions of 28 DEG C of constant temperature, conventionally planted to transplanting after 4 leaves to crop field after maize seedling is long
It plants, is then strictly screened and rejected hybrid strain, select fine individual plant to be selfed to get waxy corn DH system is arrived；By selecting waxy corn
Kind plant is maternal to lure with waxy corn monoploid tailored version induction Xi Ji section 115 to be hybridized to obtain F1 generation and double to handle and pass through
It crosses stringent screening and selfing obtains waxy corn DH system, there is excellent self-fruitfulness, can be used for the breeding of waxy corn, be conducive to
Quickly breeding goes out the more excellent good quality and high output New Waxy Maize Hybrids with excellent Ear weight.
Embodiment 4
A kind of breeding method of waxy corn DH system, it specifically includes the following steps:
1) it selects excellent variety to hybridize: being maternal lucky with waxy corn monoploid tailored version induction system with excellent white glutinous corn variety plant
Section lures 115 to be hybridized to obtain F1 generation, and the seed sodium-chloride water solution of F1 generation is then used nothing in 28 DEG C of immersion 8h again later
Bacterium water is rinsed well, and the seed A that embryo portion has purple to mark without purple label, aleurone is filtered out；The glutinous corn variety is capital
Section glutinous 2000；The glutinous corn variety is commercial product；The concentration of the sodium-chloride water solution is 0.18mol/L；The chlorine
The additional amount for changing sodium water solution is subject to submerges seed just；The time of the flushing is 20min；
2) it doubles to handle: by seed A obtained in step 1) with the seed-soaking liquid containing pendimethalin, Tween 80 and thiophene ketone at 30 DEG C
Processing 28h is doubled, is then cleaned up with deionized water, processing seed B must be doubled；Pendimethalin and tween in the seed-soaking liquid
80 mass fraction is that the mass fraction of 0.016%, thiophene ketone is 0.005%；The additional amount of the seed-soaking liquid is with lucky submergence kind
Subject to son；The time of the cleaning is 20min；
3) selfing seed selection: processing seed B will be doubled obtained in step 2 and conventionally plants and forms plant, is then selected
Female tassel all fertile single plants carry out bagging selfing, and it is more after fruit ear harvest to select and remain full seed, marshalling and tassel row number
Fruit ear carry out threshing and obtain single fringe seed C；
4) stability is cultivated: list fringe seed C obtained in step 3) being placed on filter paper, sprays distilled water and in illumination
4500Lx, vernalization is carried out under the conditions of 28 DEG C of constant temperature, conventionally planted to transplanting after 4 leaves to crop field after maize seedling is long
It plants, is then strictly screened and rejected hybrid strain, select fine individual plant to be selfed to get waxy corn DH system is arrived；By selecting waxy corn
Kind plant is maternal to lure with waxy corn monoploid tailored version induction Xi Ji section 115 to be hybridized to obtain F1 generation and double to handle and pass through
It crosses stringent screening and selfing obtains waxy corn DH system, there is excellent self-fruitfulness, can be used for the breeding of waxy corn, be conducive to
Quickly breeding goes out the more excellent good quality and high output New Waxy Maize Hybrids with excellent Ear weight.
Embodiment 5
A kind of breeding method of waxy corn DH system, it specifically includes the following steps:
1) select excellent variety to hybridize: be maternal with excellent white glutinous corn variety plant is with the induction of waxy corn monoploid tailored version
Ji Ke lures 115 to be hybridized to obtain F1 generation, then uses the seed sodium-chloride water solution of F1 generation again later in 28 DEG C of immersion 8h
Aseptic water washing is clean, filters out the seed A that embryo portion has purple to mark without purple label, aleurone；The glutinous corn variety is
Capital section glutinous 2000；The glutinous corn variety is commercial product；The concentration of the sodium-chloride water solution is 0.18mol/L；It is described
The additional amount of sodium-chloride water solution is subject to submerges seed just；The time of the flushing is 20min；
2) it doubles to handle: by seed A obtained in step 1) with the seed-soaking liquid containing pendimethalin, Tween 80 and thiophene ketone at 30 DEG C
Processing 28h is doubled, is then cleaned up with deionized water, processing seed B must be doubled；Pendimethalin and tween in the seed-soaking liquid
80 mass fraction is that the mass fraction of 0.016%, thiophene ketone is 0.005%；The additional amount of the seed-soaking liquid is with lucky submergence kind
Subject to son；The time of the cleaning is 20min；
3) selfing seed selection: processing seed B will be doubled obtained in step 2 and conventionally plants and forms plant, is then selected
Female tassel all fertile single plants carry out bagging selfing, and it is more after fruit ear harvest to select and remain full seed, marshalling and tassel row number
Fruit ear carry out threshing and obtain single fringe seed C；
4) stability is cultivated: list fringe seed C obtained in step 3) being placed on filter paper, sprays distilled water and in illumination
3500Lx, vernalization is carried out under the conditions of 28 DEG C of constant temperature, conventionally planted to transplanting after 4 leaves to crop field after maize seedling is long
It plants, is then strictly screened and rejected hybrid strain, select fine individual plant to be selfed to get waxy corn DH system is arrived；By selecting waxy corn
Kind plant is maternal to lure with waxy corn monoploid tailored version induction Xi Ji section 115 to be hybridized to obtain F1 generation and double to handle and pass through
It crosses stringent screening and selfing obtains waxy corn DH system, there is excellent self-fruitfulness, can be used for the breeding of waxy corn, be conducive to
Quickly breeding goes out the more excellent good quality and high output New Waxy Maize Hybrids with excellent Ear weight.
Embodiment 6
A kind of breeding method of waxy corn DH system, it specifically includes the following steps:
1) select excellent variety to hybridize: be maternal with excellent white glutinous corn variety plant is with the induction of waxy corn monoploid tailored version
Ji Ke lures 115 to be hybridized to obtain F1 generation, then uses the seed sodium-chloride water solution of F1 generation again later in 28 DEG C of immersion 8h
Aseptic water washing is clean, filters out the seed A that embryo portion has purple to mark without purple label, aleurone；The glutinous corn variety is
Capital section glutinous 2000；The glutinous corn variety is commercial product；The concentration of the sodium-chloride water solution is 0.18mol/L；It is described
The additional amount of sodium-chloride water solution is subject to submerges seed just；The time of the flushing is 20min；
2) it doubles to handle: by seed A obtained in step 1) with the seed-soaking liquid containing pendimethalin, Tween 80 and thiophene ketone at 30 DEG C
Processing 28h is doubled, is then cleaned up with deionized water, processing seed B must be doubled；Pendimethalin and tween in the seed-soaking liquid
80 mass fraction is that the mass fraction of 0.016%, thiophene ketone is 0.005%；The additional amount of the seed-soaking liquid is with lucky submergence kind
Subject to son；The time of the cleaning is 20min；
3) selfing seed selection: will double processing seed B and conventionally plant and form plant obtained in step 2, selection male and female
Fringe all fertile single plants carry out bagging selfing, and select and remain after fruit ear harvest the fruit more than full seed, marshalling and tassel row number
Fringe carries out threshing and obtains single fringe seed C；
4) stability is cultivated: list fringe seed C obtained in step 3) being placed on filter paper, sprays distilled water and in illumination
5500Lx, vernalization is carried out under the conditions of 28 DEG C of constant temperature, conventionally planted to transplanting after 4 leaves to crop field after maize seedling is long
It plants, is then strictly screened and rejected hybrid strain, select fine individual plant to be selfed to get waxy corn DH system is arrived；By selecting waxy corn
Kind plant is maternal to lure with waxy corn monoploid tailored version induction Xi Ji section 115 to be hybridized to obtain F1 generation and double to handle and pass through
It crosses stringent screening and selfing obtains waxy corn DH system, there is excellent self-fruitfulness, can be used for the breeding of waxy corn, be conducive to
Quickly breeding goes out the more excellent good quality and high output New Waxy Maize Hybrids with excellent Ear weight.
The corn variety of high yield and high quality can be quickly obtained using haploid breeding technology, currently, in conventional corn list
Mainly make induction system using the Stock6 derived from the U.S. in times sport kind and hybridized induction Haploid production is carried out to female parent material, so
And its inducing effect in waxy corn monoploid is cultivated is unsatisfactory, doubling monoploids rate is low, and DH system robustness is poor；The present invention
By selecting glutinous corn variety plant to induce Xi Ji section to lure 115 to be hybridized to obtain F1 with waxy corn monoploid tailored version to be maternal
In generation, doubles to handle and obtains waxy corn DH system by stringent screening and selfing, has excellent self-fertility in waxy corn cultivation
Property, it can be used for the breeding of waxy corn, be conducive to quickly breeding and go out the more excellent glutinous jade of good quality and high output with excellent Ear weight
Rice new varieties reduce human and material resources consumption, have a vast market foreground.
The above description of the embodiments is intended to facilitate ordinary skill in the art to understand and use the invention.
Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein general
Principle is applied in other embodiments without having to go through creative labor.There is no need and unable to give all embodiments
With exhaustion.Therefore, the present invention is not limited to the above embodiments, and those skilled in the art's announcement according to the present invention does not depart from this hair
The improvement and modification that bright scope is made all should be within protection scope of the present invention.
Claims (10)
1. a kind of breeding method of waxy corn DH system, which is characterized in that it specifically includes the following steps:
1) it selects excellent variety to hybridize: being that female parent is lured with waxy corn monoploid tailored version with excellent white (Huang) glutinous corn variety plant
Dao Xiji section lures 115 to be hybridized to obtain F1 generation, then by the seed sodium-chloride water solution of F1 generation in 28 DEG C of immersion 8h, later
It is clean with aseptic water washing again, filter out the seed A that embryo portion has purple to mark without purple label, aleurone；
2) it doubles to handle: by seed A obtained in step 1) with the seed-soaking liquid containing pendimethalin, Tween 80 and thiophene ketone at 30 DEG C
Processing 28h is doubled, is then cleaned up with deionized water, processing seed B must be doubled；
3) selfing seed selection: processing seed B will be doubled obtained in step 2 and conventionally plants and forms plant, is then selected
Female tassel all fertile plant carry out bagging selfing, and it is more after fruit ear harvest to select and remain full seed, marshalling and tassel row number
Fruit ear carry out threshing and obtain single fringe seed C；
4) stability is cultivated: list fringe seed C obtained in step 3) being placed on filter paper, sprays distilled water and in illumination 3500-
5500Lx, vernalization is carried out under the conditions of 28 DEG C of constant temperature, conventionally planted to transplanting after 4 leaves to crop field after maize seedling is long
It plants, is then strictly screened and rejected hybrid strain, select fine individual plant to be selfed to get waxy corn DH system is arrived.
2. the breeding method of waxy corn DH according to claim 1 system, which is characterized in that in step 1), the waxy corn
Kind be change glutinous 930, the spring is No. 1 glutinous, Kennian No.1, osmanthus are glutinous 519, first glutinous No.1, Xi Xinghuang it is No. 6 glutinous, in glutinous No.1 or capital section it is glutinous
One of 2000.
3. the breeding method of waxy corn DH according to claim 2 system, which is characterized in that in step 1), the sodium chloride
The concentration of aqueous solution is 0.18mol/L.
4. the breeding method of waxy corn DH according to claim 3 system, which is characterized in that in step 1), the sodium chloride
The additional amount of aqueous solution is subject to submerges seed just.
5. the breeding method of waxy corn DH according to claim 4 system, which is characterized in that in step 1), the flushing
Time is 15-25min.
6. the breeding method of waxy corn DH according to claim 1 system, which is characterized in that in step 2, the seed-soaking liquid
The mass fraction of middle pendimethalin and Tween 80 be 0.012-0.018%, thiophene ketone mass fraction be 0.002-0.007%.
7. the breeding method of waxy corn DH according to claim 6 system, which is characterized in that in step 2, the seed-soaking liquid
Additional amount be subject to and submerge seed just.
8. the breeding method of waxy corn DH according to claim 6 or 7 system, which is characterized in that in step 2, the cleaning
Time be 15-25min.
9. the breeding method of waxy corn DH according to claim 1 system, which is characterized in that in step 4), the stringent sieve
Hybrid strain is selected and rejected, fine individual plant is selected to be selfed.
10. a kind of application of breeding method of the waxy corn DH system as described in claim 1-9 is any in glutinous corn breeding.
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