KR20220168198A - New strawberry variety having decaploids using embryo culture and polyploid breeding between different species - Google Patents

Abstract

The present invention relates to a new decaploid strawberry variety using polyploidy breeding and embryo culture between different varieties, more specifically, which is obtained by artificially crossing Sakura strawberry variety, which is a cultivated strawberry (Fragaria ananassa Duch.), as a mother plant, and Yellow Wonder variety, which is a wild-type strawberry (F. vesca f. semperflorens), as a father plant. The new strawberry variety JY-1 with a decaploid genome is resistant to pests and diseases and has a strong fruit aroma compared to the Sakura strawberry variety, and is resistant to pests and diseases and has increased fruit size compared to the Yellow Wonder variety.

Description

New strawberry variety having decaploids using embryo culture and polyploid breeding between different species}

The present invention relates to a novel decadal strawberry cultivar using hybridization between heterogeneous cultivars and ploidy breeding.

Strawberry Fragaria ananassa Duch. (2n=8x=56), which is currently cultivated worldwide, is a cross between F. chiloensis , a wild species in South America, and F. virginiana , a wild species in North America. Breeding of cultivars through systematic crossbreeding has resulted in the genotypes of currently cultivated cultivars being limited.

Attempts to introduce new varieties and new genotypes from wild species in relation to taste, aroma, color, disease resistance, etc. are being made in many countries, but wild strawberries are mainly diploid and due to mating incompatibility with the currently cultivated strawberry octoploid. Not easy to use

Fruits of cultivars that are currently being cultivated are mostly grown in red, and some white fruits that have been abandoned due to mutations have recently been developed in Japan. To further diversify the color, aroma and flavor of strawberry berries, it is necessary to use new genetic resources in the wild.

Using wild strawberries and currently cultivated strawberries, it is a new type of strawberry that is resistant to diseases and pests of wild strawberries and is used to make strawberries with high ploidy, flavor, color and unique taste. is making

Wild varieties have small fruits and are almost impossible to cross with cultivated strawberries due to chromosomal differences. In order to make wild strawberries as large as cultivated strawberries, biotechnology (cultivation) and cell genetic engineering (polyploidization) techniques must be applied.

On the other hand, Korean Patent Publication No. 2013-0137564 discloses 'new strawberry porridge and its breeding method', and Korean Patent Publication No. 2020-0071242 discloses 'anthrax-resistant strawberry variety NSR090201 and its breeding method'. There is no description of the 'decadoploid strawberry cultivar using heterogeneous culturing and ploidy breeding' of the present invention.

The present invention was derived from the above request, and the present inventors took the cultivar, the octoploid, white fruit color Sakura Strawberry as a model, and the diploid wild strawberry, Yellow Wonder, which has yellow fruit color Using the cultivar as the parent, each chromosomal ploidy individual was prepared and then crossed, and the embryo of the immature seed obtained after the cross was cultured to grow a new decapod variety of strawberry. The present invention was completed by confirming that the grown 10-plot new variety of strawberry was resistant to pests and diseases compared to the parent, the color of the fruit was yellow and different from the parent, the fragrance of the fruit was stronger than that of the parent, and the size of the fruit was increased compared to that of the parent. did

In order to solve the above problems, the present invention is a cultivated strawberry ( Fragaria ananassa Duch.), a cherry strawberry variety as a model, and a wild type strawberry ( F. vesca f. semperflorens ), Yellow Wonder (Yellow wonder) variety as a parent It is obtained by artificial breeding, and is more resistant to pests than the cherry strawberry variety and has a strong fruit fragrance, and is resistant to pests and diseases compared to the Yellow Wonder variety and has an increased fruit size. JY-1, a new strawberry variety with a decaploid genome, is provided. .

In addition, the present invention provides an F ₁ strawberry produced by crossing a plant of the new strawberry variety JY-1 with another strawberry variety.

In addition, the present invention provides a processed strawberry food prepared using the new strawberry variety JY-1.

In addition, the present invention is a cultivated strawberry ( Fragaria ananassa Duch.), a cherry strawberry variety as a model, and a wild strawberry ( F. vesca f. semperflorens ) Yellow wonder (Yellow wonder) variety as a parent, artificially breeding them It provides a method for breeding deciduous strawberries, including a.

The new strawberry cultivar according to the present invention has increased disease and pest resistance compared to the parent, a difference in color and flavor of the fruit compared to the parent, increased fruit size compared to the parent, and capable of asexual reproduction. In addition, the new strawberry variety of the present invention has high ploidy and can contribute to providing genetic diversity of strawberry resources.

Figure 1 is a photograph showing the appearance of callus organic through the embryo culture process after crossbreeding cherries and yellow wonder varieties.
Figure 2 shows the process of regenerating the object from the leaf piece of the applied variety.
Figure 3 is the result of analyzing the size of the guard cell of the parent and the applied cultivar, and the size of about 50 guard cells for each plant was measured. Yellow Wonder, the female parent, has a guard cell size of 24 to 27 μm, with a size of 25 μm being representative, and the parent, cherry strawberry, having a guard cell size of 30 to 33 μm, with a representative cell size of 32 μm, The applied cultivar, JY-1, has a guard cell size of 35 to 38 μm, and the size of 36 μm is representative (magnification: 1,000X).
4 is a photograph of the plant of the application variety.
Figure 5 is a leaf type photograph of the parent model and the applied cultivar.
Figure 6 is a picture of the fruit of the parent model and the application variety.

In order to achieve the object of the present invention, the present invention is cultivated strawberry ( Fragaria ananassa Duch.), the cherry strawberry variety as the model, and the wild type strawberry ( F. vesca f. semperflorens ), the yellow wonder (Yellow wonder) variety as the parent model JY-1, a new strawberry variety with a decaploid genome, which is obtained by artificial breeding, and is resistant to pests and has a strong fruit fragrance compared to the Sakura strawberry variety and has increased fruit size and is resistant to pests and diseases compared to the Yellow Wonder variety. to provide.

The new strawberry variety JY-1 having a decaploid genome according to the present invention may have its growth point accession number KCTC14569BP.

Sakura strawberry, the parent used for breeding the new strawberry variety JY-1 of the present invention, is an octoploid cultivated strawberry registered as a variety in Japan in 2014 [天使の實, 天使のイチゴ (2n=8x=56)], The color of the fruit is white, and the color of the flesh is white in the winter cultivation, when the temperature is high, but the flesh color changes from white to pink in the spring cultivation when the temperature rises.

In addition, the yellow wonder variety used in the present invention is a diploid (2n = 2x = 14) wild strawberry. It is characterized by a short cycle of 3.0 to 3.5 months, no runners, and yellow fruit color. It is mainly used for ornamental purposes because it has small fruits and is not commercially available, and is distributed throughout Europe, Asia, North and South America.

The two varieties, the parent and the parent according to the present invention, are heterogeneous and do not cross well due to differences in chromosomes. In crossbreeding between species, even if embryo development stops after fertilization or seeds are obtained, it is very difficult to obtain seedlings. After a certain period of time after fertilization, seeds are formed, but in most cases, the belly of the seed is brown and dead.

In the present invention, in order to overcome the mating incompatibility between the parent varieties, embryo culture was continuously attempted to succeed in the composition of the medium. When the two varieties are crossed, fertilization does not work well, but after a certain period of time, seeds are formed, but the embryos are all browned and dead. To overcome this, embryo culture was performed on immature embryos. In addition, it was confirmed that the parent version of the present invention was not modified when reversed. It was inferred that this is because the pistil of the octaploid sakura strawberry accepts the small pollen of the diploid yellow wonder cultivar, but in the opposite case, the yellow wonder pistil is too small to accept the pollen of the cherries strawberry.

The present invention also provides an F ₁ strawberry produced by crossing a plant of a new strawberry variety JY-1 whose growing point accession number is KCTC14569BP with another strawberry variety. The new strawberry variety JY-1 according to the present invention has a dechaploid genome, and can be used as a novel genetic resource for cultivating strawberry varieties with various tastes, quantities, and fruit colors.

The present invention also provides a processed strawberry food prepared using a new strawberry variety JY-1 whose growth point accession number is KCTC14569BP. The strawberry processed food may be a beverage including jam, juice, ice cream, dairy products, etc., but is not limited thereto.

The present invention also relates to a cultivated strawberry ( Fragaria ananassa Duch.), a cherry strawberry variety, as a model, and a wild-type strawberry ( F. vesca f. semperflorens ), a yellow wonder (Yellow wonder) variety as a parent, artificially breeding them It provides a method for breeding deciduous strawberries, including a.

The method for breeding decaploid strawberries according to the present invention is specifically,

(a) multiplying the seeds of the parent and parent cultivars, Cherry Strawberry and Yellow Wonder;

(b) inducing plants from the seeds puppled in step (a);

(c) artificially crossing the parent variety and the parent variety at the flowering time of the polyploid plant induced in step (b);

(d) harvesting immature seeds 20 to 30 days after mating and then splitting and extracting embryos; and

(e) regenerating plants by culturing the embryos extracted in step (d);

In the present invention, the term 'polyploidization' means that the number of chromosomes becomes ploidy. It has been reported that polyploids increase yield, improve resistance to environmental stress and disease, and increase the content of useful secondary metabolites in plants compared to the original plant. Breeding materials have been developed.

In the breeding method according to the present invention, the doubling method of step (a) may be performed by colchicine treatment, but is not limited thereto.

In addition, in the above breeding method, the plants derived from the doubled seeds in step (b) and the deciploid strawberries bred in step (e) are chromosome (genome) through measurement of cell size or flow cytometry analysis. drainage can be checked. The size of the guard cell of the hexaploid plant (2n = 16x = 112) in which the parent cherry tree of the present invention is polyploided is about 37 to 42㎛, and 39㎛ is representative, and the yellow wonder variety, which is the parent strain, is polyploided. The size of guard cell of tetraploid plants (2n=4x=28) is about 30~33㎛, and 32㎛ is representative.

The increase in guard cell size according to plant polyploidization was reported by Silva et al. (Ciencia Rural, 2000, 30;105-111), Miguel and Leonhardt (Scientia Horticulturae, 2011, 130;314-319), and Li et al. (Acta Horticulturae Sinica, 2013). , 40;2033-2038) are known in the art.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are only to illustrate the present invention, and the content of the present invention is not limited to the following examples.

Example 1. Optimal medium composition experiment for embryo culture

For embryo culture for cultivating a new variety of strawberry, an optimal medium composition for inducing callus from immature seed-derived embryos was tested. After adding sucrose 3% (w/v), agar 7g/L, and the growth regulators shown in Table 1 below to the MS medium, the medium was adjusted to pH 5.8, and then the pears were taken out from immature seeds and placed on the medium. The level of callus production was analyzed while culturing at 25 ° C. under a 16-hour daylight condition. It was subcultured to the same medium at 2-week intervals and observed for a total of 6 weeks.

Callus production rate according to hormones MS badge Rate of production of milky white callus* BA (/L) 2,4-D (/L) Kinetin (/L) 0.1mg 0.5mg x 1.0mg + 3.0mg ++ 0.5mg 0.5mg ++ 1.0mg +++ 3.0mg ++++ 1.0mg 0.5mg +++ 1.0mg +++ 3.0mg +++ 0.5mg 0.5mg 0.1mg +++ 1.0mg 0.1mg +++ 3.0mg 0.1mg ++++ * x, none; +, very little (~5%); ++, moderate (~30%); +++, good (~50%);
++++, very good (greater than 80%).

As a result, it was found that milky white callus was formed in the form of lumps after about 4 weeks (Fig. 1), and medium conditions containing 0.5 mg/L BA, 3.0 mg/L 2.4-D and 0.1 mg/L KT It was found that the callus production rate was excellent. In addition, the present inventors prepared media containing TDZ and 2,4-D at various concentrations for inducing shoots from callus (Table 2), and then transferred the milky white lumpy callus to each medium and Firepower was analyzed. As a result, it was found that shoot formation began 4 to 6 weeks after the callus was transferred to each medium, and the shoot differentiation capacity was improved in the medium containing 2.0 mg/L TDZ and 0.5 mg/L 2,4-D. found to be the best.

New shoot differentiation potential according to hormone concentration MS badge Shoot differentiation power (%) TDZ (/L) 2,4-D (/L) 1.0mg 0.5mg 25% 1.0mg 1.0mg 30% 1.5mg 0.5mg 55% 1.5mg 1.0mg 65% 2.0mg 0.5mg 100% 2.0mg 1.0mg 80% 2.5mg 0.5mg 75% 2.5mg 1.0mg 50%

Based on the above results, in the present invention, MS medium containing 0.5mg/L BA, 3.0mg/L 2.4-D and 0.1mg/L KT during callus induction during embryo culture, and 2.0mg/L TDZ and 2.0mg/L TDZ during shoot induction MS medium containing 0.5mg/L 2,4-D was used, and after shoot formation, it was transferred to MS medium containing 0.2 mg/L BA and 0.05 mg/L IBA for growth, and then 0.2 mg/L IBA. Rooting was induced by transferring to 1/2 MS medium containing .

Example 2. Decaploid Strawberry upbringing

In order to grow the decaploid strawberry lineage, the chromosomes of the parent variety were each doubled, then crossed and grown through an embryo culture process.

(2-1) Polyploidization of Yellow wonder variety

(2-1-1) Soak the seeds of the Yellow Wonder variety (purchased from https://www.amazon.co.jp; 不思議なイエロ一イチゴ種) in detergent water for about 20 minutes, then wash in running water for about 30 minutes. and soaked in 75% (v/v) alcohol for about 1 minute in a clean bench, washed with sterilized water, soaked in sodium hypochlorite (NaOCl) for 10 minutes, washed with sterilized water, and washed again with chlorine After sterilizing in mercury (HgCl ₂ ) for about 10 minutes, washing with sterilized water about 3 times, and immersing in sterilized water for about 3 hours.

(2-1-2) Treatment of seeds, leaves or growing points with colchicine in flight

- Seed colchicine treatment method

Sterilize the seeds by the method of 1-1, open the seeds under a microscope in a sterile test bench, spread a sterilized filter paper on a sterilized petri dish, pour sterilized water containing 0.05 ~ 0.3% (w / v) of colchicine, and pour the filter paper Seeds were planted on top. This process was repeated repeatedly. After 7-14 days, the germinated individuals were transferred to 1/2 MS medium containing 1% (w/v) sucrose and grown.

- Growing point colchicine treatment method

The seeds were sterilized by the method of 1-1, and the seeds were separated after absorbing moisture with a sterilized filter paper under a biological microscope. After mixing 6g of agar with 1L of distilled water, autoclave, dispensing 40ml each into a sterilized petri dish (90ø × 20mm) and hardening it, and then seeding the seeds separately and sealing the petri dish under light conditions. It was cultured under 16-hour daylight conditions and 25°C temperature conditions. After germination, the individual was transferred to 1/2 MS liquid medium containing 1% (w/v) sucrose and grown for 5 to 10 weeks, then the growth points were collected and shaken cultured in liquid medium containing 0.3% colchicine for 5 days. After washing with sterilized water three times, and immersed in sterilized water for about a day, the objects were propagated after being placed on 1/2 MS medium. In parallel, the growing point was plated on a solid 1/2 MS medium containing 1% (w/v) sucrose and 200-300 mg/L colchicine, cultured for about 30 days, and then transferred to a solid 1/2 MS medium for proliferation. did

- Leaf colchicine treatment method

Leaf pieces from the colchicine treatment of the growing point were planted on MS medium containing 2.0 mg/L TDZ (thidiazurone), 0.1 mg/L 2,4-D (2,4-Dichlorophenoxyacetic acid) and 300 mg/L colchicine for 10 days After dark culture, it was transferred to light culture, and after 20 days, it was transferred to MS medium containing TDZ and 2,4-D except for colchicine. After 2 weeks, they were transferred to the same medium again.

As callus is formed, shoots begin to occur after 5 to 7 weeks, and at this time, they are transferred to 1/2 MS medium for proliferation. Due to the influence of colchicine, shoots do not develop well and it takes a long time to induce shoots. The Yellow Wonder variety is a wild species and does not require a lot of nutrients, so it grows well with about half of the MS medium and also grows well with sucrose at a concentration of 1% (w / v). Perform the above operation repeatedly.

(2-1-3) Analysis of guard cell size, confirmation of chloroplast number, and selective proliferation

The size of the guard cells of the individuals proliferated by treating seeds, leaflets, and growing points in vitro with colchicine was analyzed using a phase-contrast microscope, and stained with an iodine-potassium iodide (I ₂ -KI) solution to determine the size of chloroplasts in the guard cells. Individuals were selected by checking the numbers. For the method of measuring guard cell size and confirming chloroplasts, refer to the methods of Ki-Hyun Kim et al. For guard cell size analysis, 2,000 individuals proliferated from seeds, leaflets or growing points were analyzed, and two plants were selected.

Yellow Wonder is a diploid (2n=2x=14), and the size of the guard cell is about 23~27㎛, but the size of the guard cell in the polyploided individual is about 28~32㎛, and the chloroplast inside the guard cell It was confirmed that the water supply increased by 1.5 to 2.0 times according to the drainage. In addition, as a result of staining with Cysrain UV P type and analyzing the number of chromosomes using a cytometer (Flow cytometry, Ploidy Analyzer UV light), it was confirmed that they were tetraploid.

(2-2) Polyploidization of Cherry Strawberry (天使のイチゴ) varieties

(2-2-1) Sakura Strawberry (White Strawberry, Strawberry Angel, 天使のイチゴ) can be found on the Internet (https://search.rakuten.co.jp/search/mall/%E7%99%BD+%E3%81% 84%E3%81%A1%E3%81%94+%E8%8B%97/). After immersing the cherry strawberry runner in detergent water for 20 minutes, washing it in running water for 30 minutes, 75% (v/v) alcohol for 30 seconds, sodium hypochlorite solution for 10 minutes, and mercury chloride for 10 minutes in a sterile laboratory. After sterilization, it was rinsed with sterile water three times and then soaked in sterile water for 3 hours. After absorbing moisture with a sterilized filter paper, growth points were collected under a microscope and placed on MS medium containing 0.2 mg BA (benzyladenine). Individuals were propagated by transferring to the same medium at 4-week intervals.

(2-2-2) Drainage work

- Treatment with liquid culture

After individual propagation in vitro, leaf pieces and growing points were collected and cultured with shaking for 2 to 5 days in a liquid medium containing 0.05 to 0.3% colchicine. After 15 days of dark culture in 1/3 MS medium containing ,4-D, it was transferred to light culture and cultured. It was transferred to MS medium and cultured in light.

- Treatment with solid culture

After individual propagation in vitro, leaf pieces and growing points are collected, and the growing points are placed on MS solid medium containing 0.2mg/L BA, 0.05mg/L IBA, and 0.01~0.05% colchicine, and cultured for 30~40 days. After that, they were subcultured in the same medium without colchicine. Leaf pieces were plated on 1/3 MS solid medium containing 2.0mg/L TDZ, 0.1mg/L 2,4-D and 0.01~0.05% colchicine, cultured in the dark for 20 days, and then transferred to the same medium without colchicine. Cultivation was carried out.

(2-2-3) Guard cell size analysis and selection proliferation

Among individuals treated with colchicine in vitro and grown in culture, the size of guard cells was measured using a phase-contrast microscope, and polyploid individuals were identified and selected and propagated. For the measurement of guard cell size, 2,000 individuals proliferated after colchicine treatment were analyzed, and one individual was finally selected.

The size of the guard cell of the octoploid cherry strawberry was 30~34㎛, but the size of the guard cell of the polyploided individual was about 36~40㎛. In addition, as a result of staining with Cysrain UV P type and analyzing the number of chromosomes using a cytometer (Flow cytometry, Ploidy Analyzer UV light), it was confirmed that they were hexaploid.

(2-3) Embryo culture after mating between multiple individuals

Crossing between a polyploid tetraploid individual of Yellow Wonder and a polyploid hexaploid individual of cherry strawberry rarely results in fertilization itself. Only a few seeds could be obtained by repeatedly attempting fertilization, but seeds were formed after a certain period of time after mating, but the embryos were all browned and dead. To overcome this, culture of immature embryos was attempted. First, immature seeds were collected 20 to 30 days after artificially insemination of both individuals. When seeds less than 20 days old were used, pears could not be collected because they were too soft (not hard), and when 30 days were exceeded, seed pears began to die, resulting in difficulty in object development.

In artificial crossbreeding between both cultivars, the petals and stamens were removed one day before the flower buds of the cherry strawberry individual were fertilized with pollen of the Yellow Wonder cultivar, the buds were wrapped with parchment paper, and the date of fertilization was recorded and the same for 3 days at 1-day intervals. The correction process was repeated repeatedly. On the 25th day after mating, immature seeds were collected, soaked in detergent water for 20 minutes, washed in running water for 30 minutes, treated with 75% (v/v) alcohol for 30 seconds in a sterile laboratory, washed with sterilized water, and washed with hypochlorous acid After 10 minutes of sodium, it was washed with sterile water, dipped in a mercuric chloride solution for 10 minutes, washed three times with sterile water, and then soaked in sterile water for 3 hours. After absorbing water with a sterilized filter paper, open the seeds using a microscope and carefully take out the pears. were deposited on the medium. Milky white callus is formed in about 4 to 6 weeks. At this time, it is transferred to MS medium containing 2.0mg/L TDZ and 0.5mg/L 2,4-D and cultivated for 3 to 4 weeks to produce shoots. start to become After shoots started to be produced, they were transferred to a growth medium (MS medium containing 0.2 mg/L BA and 0.05 mg/L IBA) and proliferated (FIG. 2).

(2-4) Selection of individuals

A series of numbers were assigned to 48 individuals proliferated through embryo culture, and in vitro propagation was performed for each individual, and after analyzing the size of guard cells for each individual, it was confirmed that all guard cell sizes were 35 to 40 μm ( 3), after the purification work, demonstration cultivation was carried out in the open field. After that, each of the above individuals was propagated by tissue culture to receive each runner (crawling), and the mother plant and runners (20 per individual) were grown in the winter greenhouse and in the spring, and there was no heterogeneous strain, Growth and fruiting appeared uniform, and one individual with a mesophilic phenotype was finally selected. The object was named JY-1, and after tissue culture of the JY-1 plant to regenerate the plant, growth points were collected and deposited at the Biological Resource Center of the Korea Research Institute of Bioscience and Biotechnology on May 13, 2021.

Example 3. Characteristics of decaploid strawberries

The cultivar characteristics of the decaploid strawberry JY-1 were investigated using the parent cherry strawberry and Yellow Wonder cultivars as control cultivars, and the criteria for evaluation were based on the <Current Characteristic Survey Guidelines for New Varietal Examination (Strawberry STRAWBERRY ( Fragaria L.))>, and the actual values were analyzed by randomly taking 20 weeks of individuals. As for analysis characteristics, fruit color, fruit type, and creeping length related to parental characteristics were mainly observed.

As a result, the applied variety, JY-1, is mesophilic and has the specificity of differentiated flower buds when a certain period of time (about 4 months) is reached regardless of day length and temperature. It was observed that the fruit was white when cultivated in a cold low temperature period, and turned yellow when the temperature rose in spring, showing a slightly pink color. It was found that both the yellow wonder and the parent had the characteristics of cherry blossoms (FIG. 4). Seed color was identified as red.

Yellow Wonder used in the breeding of the application variety of the present invention did not develop runners, but the applied variety JY-1 developed runners and was able to reproduce by children. In addition, it was observed that the applied cultivar had thicker and larger leaves and a thicker wax layer than the parent model (FIG. 5).

In addition, as a result of examining the insect resistance of the applied variety and parent in the field in 2020, it was found that the applied variety JY-1 significantly reduced the occurrence of aphids and mites compared to the parent (Table 3).

pest resistance Sakura Strawberry yellow wonder JY-1 aphid ++ +++ + Mite ++ ++ + -: no occurrence, +: slight occurrence, ++: moderate occurrence, +++: severe occurrence, ++++: very severe occurrence.

In addition, as a result of performing a sensory test on 50 ordinary people, it was found that the preference for the applied variety, JY-1, was excellent (Table 4).

sensory test result Sakura Strawberry yellow wonder JY-1 preference 0 3 people 47 people ratio 0 6% 94% It was confirmed that the fragrance was excellent in the sensory test.

The applied variety of the present invention has excellent marketability due to its large fruits compared to the parent, which has small fruit and no marketability, and has excellent productivity because it is resistant to insect damage compared to the parent, and has a stronger fruit fragrance and different color of the fruit than the parent. It will be distributed as a strawberry variety.

Korea Research Institute of Bioscience and Biotechnology KCTC14569BP 20210513

Claims (6)

Cherry strawberry variety, cultivated strawberry ( Fragaria ananassa Duch.), as a model, and wild-type strawberry ( F. vesca f. semperflorens ), which is obtained by artificially crossing Yellow wonder variety, as a parent. JY-1, a new strawberry cultivar with a decaploid genome that is resistant to pests and has a strong fruit fragrance, is resistant to pests and diseases compared to Yellow Wonder, and has an increased fruit size. The new strawberry variety JY-1 according to claim 1, characterized in that the accession number of the growth point derived from the new strawberry variety JY-1 is KCTC14569BP. An F ₁ strawberry produced by crossing a plant of the new strawberry variety JY-1 of claim 1 with another strawberry variety. Strawberry processed food prepared using the new strawberry variety JY-1 of claim 1. Cultivated strawberry ( Fragaria ananassa Duch.), cherry strawberry variety as a model, and wild strawberry ( F. vesca f. semperflorens ) Yellow wonder (Yellow wonder) variety as a parent, including the step of artificially crossing them, 10 Breeding method of diploid strawberry. According to claim 5,
(a) multiplying the seeds of the parent and parent cultivars, Cherry Strawberry and Yellow Wonder;
(b) inducing plants from the seeds puppled in step (a);
(c) artificially crossing the parent variety and the parent variety at the flowering time of the polyploid plant induced in step (b);
(d) harvesting immature seeds 20 to 30 days after mating and then splitting and extracting embryos; and
(e) regenerating plants by culturing the embryos extracted in step (d);

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