CN105123495A - Application of partially indica-type paddy rice dominant dwarf mutant - Google Patents
Application of partially indica-type paddy rice dominant dwarf mutant Download PDFInfo
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- CN105123495A CN105123495A CN201510418415.5A CN201510418415A CN105123495A CN 105123495 A CN105123495 A CN 105123495A CN 201510418415 A CN201510418415 A CN 201510418415A CN 105123495 A CN105123495 A CN 105123495A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses an application of a partially indica-type paddy rice dominant dwarf mutant and belongs to the technical field of crop breeding. In the invention, natural mutation of genome of paddy rice, namely, a dominant dwarf mutant plant, is employed to obtain a stable and pure dominant dwarf mutant material through pollen tissue cultivation, thereby providing a dwarf source for breeding two-line or three-line hybridized paddy rice varieties. The selected dominant dwarf mutant can significantly reduce plant height of paddy rice, can improve anti-lodging performance and stable yield, and increases possibility of selecting an inter-subspecific hybridization variety and further provides substance conditions of increasing yield of the paddy rice and converting farming modes. With the stable and pure dominant dwarf material, a system being capable of randomly grouping and selecting the dwarf hybridization paddy rice varieties is established, by which the lodging problem, which often occurs during production, is solved. Meanwhile, the dominant dwarf mutant is suitable for a simplified cultivation mode in production of hybridized seeds and in large field, and can reduce labor intensity and production cost.
Description
Technical field
The invention belongs to rice breeding technology field, be specifically related to a kind of application of indica-tendency type rice dominant dwarf mutant.
Background technology
Plant height is the Main Agronomic Characters relevant to output, according to the plant height of rice maturity, usually paddy rice is divided into high stalk, semi-dwarf mutant and three types of short stem, plant height exceed certain limit easily cause lodging and the underproduction, of short stem be not only conducive to resistant to lodging, and impoverishment tolerant, be conducive to improving output.The breeding wheat for semidwarfness that late 1950s starts, makes Plant Height of Rice be reduced to semi-dwarf mutant, and rice yield obtains first time leap, one of significant achievement becoming " green revolution ".Current ongoing Super rice breeding mainly utilizes ideotype and subspecies indica and japonica hybrid advantage to be further enhanced to rice yield.
At present, long-grained nonglutinous rice breeding mostly utilizes sd-1 Semi dwarfism gene, is also much to import sd-1 gene by indica-japonica hybrid in japonica rice breeding of short stem, or utilizes the short source of japonica rice with sd-1 gene.The Reusability of same gene, by causing, the hereditary basis of improved variety is narrow, and hereditary fragility will increase.And the dwarf gene utilized in rice breeding is all recessive gene, in breeding of hybridized rice, the use of Recessive dwarf genes often requires that parents must have same dwarf gene, which limits and select favourable parent in resource widely.If but utilized indication law, then Rice Germplasm Resources could be made to be used more effectively and to shorten breeding time.Therefore new Plant Height of Rice controlling gene particularly indication law is excavated, significant to improving rice yield further.
Make full use of the merits such as the nanism of dominant dwarf and binding molecule marker assisted selection means, male sterile line and the restorer of dominant Dwarfing gene are carried in seed selection, innovation breeding material, to improveing the plant height of existing hybrid rice variety, improves the anti-fall performance of hybrid rice variety.
Summary of the invention
Technical problem
At present the Rice Dwarf key-gene of extensive use can only have sd-1 aborning, and the key-gene of short stem of other type or with bad gene close linkage, or with other bad proterties, as grown thickly, thin stalk, dew joint, granule etc., all do not obtain aborning and effectively utilize, the object of this invention is to provide indica-tendency type rice dominant dwarf, its nanism and other excellent economical characters can be made full use of, and binding molecule marker assisted selection means, seed selection is carried three of dominant Dwarfing gene and is or two is restorer and male sterile line, to improveing the plant height of existing hybrid rice variety, improve the anti-fall performance of hybrid rice variety.
Technical scheme
A kind of application of indica-tendency type rice dominant dwarf mutant, it is characterized in that, this indica-tendency type rice dominant dwarf mutant is dominant short bar sd-y, paddy rice (Oryzasativa), preservation is carried out at China Committee for Culture Collection of Microorganisms's common micro-organisms center on May 7th, 2015, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address institute of microbiology of the Chinese Academy of Sciences, preserving number is CGMCCNO:10593.
Described dominant short bar sd-y can be for two or the cultivation of series of three-series hybrid rice kind provides the application in short source.With dominant short bar sd-y for male parent, recover to be male parent with rice male, the plant height of improvement rice restorer.
Described dominant short bar sd-y can application in the production of hybrid seeds or the light simple cultivation mode of Production of Large Fields, or is applied in raising hybrid rice lodging resistance kind.
Beneficial effect
The lodging sex chromosome mosaicism of rice varieties is own through becoming the important limiting factor of Rice Production and rice variety selective.Lodging not only causes Yield of Rice Varieties to decline, but also causes the change of rice quality bad.Affecting among the relevant factors of the anti-fall property of rice varieties, plant height is one of most important factor.
The indica type dominant dwarf sd-y of institute of the present invention seed selection shows dwarfing (Fig. 1) from seedling stage, performance in tillering stage is downgraded and is tillered and increases (Fig. 2), be only as 49.27cm to maturing stage sd-y plant height, less than the half (Fig. 3) of wild type plant height 98.47cm.Except Dwarfing phenotypes, also performance blade is dark green increases with tillering.Tillering time ripe is approximately 1.4 times of wild type.Compared with wild type, each internode shorten in average of sd-y is the mutant of dn type.
With the indica type dominant dwarf sd-y of institute of the present invention seed selection for maternal (plant height 49.27cm), be about 100cm with the plant height that Dular, Kasalath, Kui rice, KN configure hybrid combination for male parent, plant height reduces amplitude and about reaches 50%.
The present invention utilizes the japonica rice line carrying dominant dwarf rice mutator gene to carry out artificial hybridization from different parent materials, effectively can reduce the plant height of the hybrid rice of institute's seed selection, improving the lodging resistance of the hybrid rice of institute's seed selection, is the resources approach that the super close problem exploration of plant height in solution rice varieties and subspecies high yield and Breeding For Super High-yielding process is new.
Accompanying drawing explanation
The plant phenotype of Fig. 1 sd-y when seedling stage
The plant phenotype of Fig. 2 sd-y when heading stage
Fig. 3 sd-y and the plant phenotype of wild type when heading stage
Fig. 4 sd-y and high stalk parent Dular and F
1plant height when heading stage
Biological deposits
Dominant short bar sd-y, paddy rice (Oryzasativa), carry out preservation on May 7th, 2015 at China Committee for Culture Collection of Microorganisms's common micro-organisms center, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address institute of microbiology of the Chinese Academy of Sciences, preserving number is CGMCCNO:10593.
Embodiment
The selection of a kind of quick acquisition indica-tendency type rice dominant dwarf mutant sd-y of the present invention, comprises the following steps:
At the rice breeding F of Jiangsu Inst. of Coastal Area Agricultural Sciences's experimental plot plantation
2(F is numbered for selective nursery
2009, be combined as Weedy Rice × salt extensive 269) the indica type Dwarf Mutants material of middle acquisition (plant height about 50cm); Self progeny's height bar to be seriously separated with of short stem, chooses the individual plant consistent with mutant plant height and phenotype, reserves seed for planting and continues plantation, utilizes the M of improvement
8medium carries out pollen tissue cultures.
Pollen tissue cultures
The minimal medium that inducing culture is used for callus induction is M
8medium, and all additional 30gL in medium
-1sucrose, 8gL
-1agar powder and 2mgL
-12,4-D (2,4-phenoxy acetic acids).Sorbierite 5gL is also addition of in inducing culture
-1, and 0.2mgL
-1kT (kinetin) and 1mgL
-1the exogenous hormones such as NAA (methyl α-naphthyl acetate).
The medium that differential medium is used for long-grained nonglutinous rice differentiation is with M
8for minimal medium, sucrose concentration is 30gL
-1with glucose 30gL
-1, agar powder 8gL
-1, 1gL
-1active carbon, additional 2mgL is set
-16-BA and 1mgL
-1kT.
Root media is all take MS as minimal medium, and sucrose concentration is 15gL
-1, agar powder 8gL
-1, 1mgL
-1iAA, 2mgL
-1pPP (paclobutrazol) 4mgL
-1colchicine.
Pretreatment
Generally at fine day, the time carries out at dusk to get fringe, chooses the tassel that pollen differentiation is in mid-late uninucleate stage, pulvinus about distance 10-15cm.Spike of rice, by 75 ﹪ alcohol wipe, wraps with gauze, puts into refrigerator by surface, 6-10 DEG C of Cold pretreatment 3 days.
The induction of Rice anther culture callus
By the tassel fetched, through 75% Ethanol Treatment 90S, soak 15min with 10% hydrogen peroxide solution, then use rinsed with sterile water 3-4 time, then the tassel of sterilizing grain husk shell is cut off, pollen is inoculated in above inducing culture.Every bottle graft kind about 100 pieces, each material does 10 bottles, cultivates under putting into light culture room (26 ± 2) DEG C dark condition.
The differentiation of Rice Callus and plant regeneration
Select the callus of well-grown, compact structure, go to differential medium, each culture dish connects 5-6 callus, cultivates under (26 ± 2) DEG C illumination condition, and light intensity is 400 μm of olm
-2s
-1, illumination every day 16h, cultivates about 30d, evoking adventive bud, and by produce the tissue of green bud go to MS be minimal medium strong seedling culture base in, cultivate under (26 ± 2) DEG C illumination condition, make it take root and grow up to whole plant.
Transplant land for growing field crops
Treat that green seedling grows to about 15cm, have many adventive root time of origins, removed test tube, under being placed on laboratory room temperature, carry out hardening, within 3 days, transplant afterwards to large field, obtain indica-tendency type rice dominant dwarf mutant sd-y.
Sd-y shows dwarfing (Fig. 1) from seedling stage, and performance in tillering stage is downgraded and tillered and increases (Fig. 2), is only 49.27cm, less than the half (Fig. 3) of wild type plant height 98.47cm to maturing stage sd-y plant height.Except Dwarfing phenotypes, also performance blade is dark green increases with tillering.Tillering time ripe is approximately 2 times of wild type.Compared with wild type, each internode shorten in average of sd-y is the mutant of dn type.Indica-tendency type rice dominant dwarf mutant sd-y, other economical characters are shown in (table 1).
Indica type dominant dwarf sd-y and wild type (public, to see national paddy rice data center http://www.ricedata.cn/index.htm) reciprocal cross F
1complete expression mutation body surface type, shows that short strain is dominant to high strain.Sd-y Inheritance Analysis on Genetic: winter in 2012 is at the F of Hainan plantation hybrid combination sd-y and Dular
2colony, at F
2investigate the individual plant heights of 575 strains in segregation population altogether, using 75cm as line of demarcation that is of short stem and high stalk, result shows that of short stem, high stalk strain number is respectively 421 and 154 strains, meet 1 pair of gene show the segregation ratio (χ of 3:1
2=1.81< χ
20.05=3.84), show that sd-y mutant phenotype is controlled by a pair dominant nuclear gene.
See the indica dominant dwarf sd-y (plant height 49.27cm) that (table 2) is with institute's seed selection, with Dular, Kasalath, Kui rice, KN (note: be public, see national paddy rice data center http://www.ricedata.cn/index.htm) positive and negative hybridization configuration combination, offspring F
1plant height be about 100cm, plant height reduce amplitude about reach 50%.
Table 1 dominant dwarf sd-y compares with wild type economical character
The indica type dominant dwarf sd-y of table 2 configures hybrid combination plant height table
M improved by table 3
8culture medium prescription
Claims (6)
1. an application for indica-tendency type rice dominant dwarf mutant, is characterized in that, this indica-tendency type rice dominant dwarf mutant is dominant short bar
sd-y, paddy rice (
oryzasativa), carry out preservation on May 7th, 2015 at China Committee for Culture Collection of Microorganisms's common micro-organisms center, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, address institute of microbiology of the Chinese Academy of Sciences, preserving number is CGMCCNO:10593.
2. application according to claim 1, is characterized in that, refers to dominant short bar according to claim 1
sd-yfor two be or the cultivation of series of three-series hybrid rice kind provides the application in short source.
3. application according to claim 1 and 2, is characterized in that, refers to dominant short bar
sd-yfor male parent, recover to be male parent with rice male, the plant height of improvement rice restorer.
4. application according to claim 1 and 2, is characterized in that, refers to described dominant short bar
sd-yapplication in the production of hybrid seeds or the light simple cultivation mode of Production of Large Fields.
5. application according to claim 1 and 2, is characterized in that, refers to described dominant short bar
sd-yimproving the application in hybrid rice lodging resistance kind.
6. application according to claim 3, is characterized in that, refers to dominant short bar according to claim 1
sd-yimproving the application in hybrid rice lodging resistance kind.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184651A (en) * | 2018-01-16 | 2018-06-22 | 江西红种业科技股份有限公司 | A kind of type rice variety selective method resistant to lodging |
| CN113273460A (en) * | 2021-06-17 | 2021-08-20 | 宁波市农业科学研究院 | Lodging-resistant conventional japonica rice breeding method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1275719A1 (en) * | 2000-03-31 | 2003-01-15 | National Institute of Agrobiological Sciences | Gene concerning brassinosteroid-sensitivity of plants and utilization thereof |
| WO2007046403A1 (en) * | 2005-10-19 | 2007-04-26 | National University Corporation Nagoya University | Plant belonging to genus oryza having dominant dwarf trait, and use thereof |
| CN101701221A (en) * | 2009-11-25 | 2010-05-05 | 天津师范大学 | Rice dwarf straw leaf roll mutant (cd1) gene and application thereof |
| CN101747420A (en) * | 2009-12-29 | 2010-06-23 | 中国科学院遗传与发育生物学研究所 | Dominant dwarf rice related protein, encoding gene thereof and application |
| WO2010076766A1 (en) * | 2008-12-30 | 2010-07-08 | Institute Of Genetics And Developmental Biology | Genes associated with plant tiller number and uses thereof |
| CN102559653A (en) * | 2011-01-02 | 2012-07-11 | 中国科学院遗传与发育生物学研究所 | Application of OsDCL3b in dwarf rice cultivation |
-
2015
- 2015-07-16 CN CN201510418415.5A patent/CN105123495B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1275719A1 (en) * | 2000-03-31 | 2003-01-15 | National Institute of Agrobiological Sciences | Gene concerning brassinosteroid-sensitivity of plants and utilization thereof |
| WO2007046403A1 (en) * | 2005-10-19 | 2007-04-26 | National University Corporation Nagoya University | Plant belonging to genus oryza having dominant dwarf trait, and use thereof |
| WO2010076766A1 (en) * | 2008-12-30 | 2010-07-08 | Institute Of Genetics And Developmental Biology | Genes associated with plant tiller number and uses thereof |
| CN101701221A (en) * | 2009-11-25 | 2010-05-05 | 天津师范大学 | Rice dwarf straw leaf roll mutant (cd1) gene and application thereof |
| CN101747420A (en) * | 2009-12-29 | 2010-06-23 | 中国科学院遗传与发育生物学研究所 | Dominant dwarf rice related protein, encoding gene thereof and application |
| CN102559653A (en) * | 2011-01-02 | 2012-07-11 | 中国科学院遗传与发育生物学研究所 | Application of OsDCL3b in dwarf rice cultivation |
Non-Patent Citations (4)
| Title |
|---|
| KENJI ASANO, ET AL.: "Isolation and characterization of dominant dwarf mutants, Slr1-d, in rice.", 《MOL GENET GENOMICS》 * |
| RUIZHEN QIN, ET AL.: "Genetic analysis of a novel dominant rice dwarf mutant 986083D", 《EUPHYTICA》 * |
| 陈峰等: "水稻矮秆基因的研究进展", 《山东农业科学》 * |
| 陈庭木等: "籼型水稻新半矮秆基因的发现与初步研究", 《杂交水稻》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184651A (en) * | 2018-01-16 | 2018-06-22 | 江西红种业科技股份有限公司 | A kind of type rice variety selective method resistant to lodging |
| CN113273460A (en) * | 2021-06-17 | 2021-08-20 | 宁波市农业科学研究院 | Lodging-resistant conventional japonica rice breeding method |
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