CN109197580A - A kind of efficient selection of good quality and high output cotton - Google Patents
A kind of efficient selection of good quality and high output cotton Download PDFInfo
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- CN109197580A CN109197580A CN201811294949.1A CN201811294949A CN109197580A CN 109197580 A CN109197580 A CN 109197580A CN 201811294949 A CN201811294949 A CN 201811294949A CN 109197580 A CN109197580 A CN 109197580A
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- cotton
- ovary
- high output
- good quality
- efficient selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
Abstract
The invention belongs to raising seedling of cotton fields, and in particular to a kind of efficient selection of good quality and high output cotton, to cotton pollen emasculation；The ovary for removing stamen is smeared using inducer；10 days ovarys expanded of Post flowering are chosen, rataria is taken out and is cultivated on MS culture medium, until after bud is grown；After seedlings root is suitable for, practice the transplanting of seedling matrix.The present invention provides a kind of methods based on mutagens inducing cotton dihaploid, it is easy to operate, megaspore doubles under the induction such as dimethyl sulfoxide, colchicine, the ovule that can normally expand is the successful euploid of induction, improve the induced efficiency of dihaploid in cotton, the hybrid vigour between sea island cotton and upland cotton can be quickly fixed, plan bottom solves the problems, such as sea Lu offspring's violent segregation.
Description
Technical field
The invention belongs to raising seedling of cotton fields, and in particular to a kind of efficient selection of good quality and high output cotton.
Background technique
Cotton is the important industrial crops in China, and long-term cultivated area is at 60,000,000 mu or so, with 200,000,000 cotton growers and 19,000,000
The employment of textile worker is closely bound up.Although traditional traditional breeding techniques is in the improvement of output of cotton, quality and resistance etc.
Major progress is achieved, has made great contribution for the improvement of people's lives and the development of national economy, but to meet China
The demand of textile industry fast development simultaneously takes part in international competition, some present in Cotton in China breeding field and Cotton Industry to ask
Topic should draw attention.It is that cotton fiber quality is poor first, especially fiber strength is relatively low, it has also become restrict Cotton in China
The significant problem of industry sustainable development.It is striven under ground aggravation of contradictions, cultivated area secondly, Cotton in China production is faced with grain and cotton
Drop, disease pest endanger the great production problem such as serious, have seriously constrained the further development of Cotton in China production.Solve above-mentioned ask
Inscribing most effective approach is to cultivate high yield, high-quality, how anti-new cotton variety.Conventional breeding methods usually require 6-8 even more
The long time, not only the period is long, but also to consume a large amount of financial resources, human and material resources, causes time and the wasting of resources.Therefore, add
The breeding process of fast new varieties, to shorten breeding time be cotton breeding technology, one of emphasis of Gonna breakthrough in method.
Method for breeding haploidy utilizes embryo rescue after Anther Culture, Unfertilized Ovaries culture and radiation pollen pollination induction
The methods of induction Haploid production, then monoploid is doubled, the single chromosome doubling for having each monoploid at
It is right, become with normal vital, can normal solid homozygote, stabilization characteristics of genetics no longer generates separation, by screening
After can be used directly as breeding material.Haploid breeding has that breeding cycle is short (obtains stable pure lines from hybridizing to
Only need the time of 2 generations), high (haploid genotype is consistent with expression power, improves major traits screening for breeding efficiency
Reliability), be a kind of breeding method being widely used on many crops at present.Haploid breeding is in grass such as rice at present
Using more on section crop and brassicaceous vegetable, mainly microspores cultures such as pollen.Cotton is induced using microspores culture
Monoploid is relatively difficult, there is no using microspore approach succeed at present, and megaspore culture induction monoploid also rarely has report
Road culture.
Summary of the invention
It is an object of the invention to solve the problems, such as that cotton haploid breeding efficiency is lower, a kind of double single times of cotton are provided
The method for creating of body can be fixed quickly between upland cotton, the hybrid vigour between land-sea, solve extra large Lu offspring violent segregation
Problem, this method is simple, efficiently, is suitable for large-scale promotion.
The specific technical proposal is:
A kind of efficient selection of good quality and high output cotton, comprising the following steps:
(1) cotton advantageous combination is configured；
(2) emasculation of F1 pollen is combined to cotton；
(3) ovary for removing stamen is smeared using inducer；
(4) 10 days ovarys expanded of Post flowering are chosen, rataria is taken out and is cultivated on MS culture medium, until after bud is grown；
(5) after seedlings root is suitable for, practice the transplanting of seedling matrix.
The efficient selection of the good quality and high output cotton, it is detailed to include the following steps
(1) the strong advantageous combination of configuration hybrid vigour, F1 generation carries out emasculation processing on the day before flowering stage is bloomed, and is listed
Label；
(2) morning on the day of blooming is stained with inducer with writing brush, is applied in the ovary for removing stamen flower repeatedly, smears
Style puts on ceratuba afterwards, prevents external source pollen, blooms second day and smears one time again, and Post flowering third day is smeared again protects bell agent；
(3) ovary expanded is taken in Post flowering the 10th day, carries out ovules culture in vitro, epidermis is cleaned up, ultra-clean
It is carried out disinfection again with mercuric chloride solution immersion after being impregnated on workbench with alcoholic solution, after aseptic water washing, blots children with aseptic paper
Fruit surface moisture carefully separates ovule with tip tweezers by young peach along ovary longitudinal incision；Ovule is inoculated in induced medium
Fiber differentiation to bud is carried out to grow；
(4) when plant root development is suitable for, start hardening, first triangle bottle closure is opened, one layer of clear water is added and is placed in
It is grown 6 hours under room temperature, then takes out plant, cleaned root culture medium, be put into triangular flask, poured into clear water and grow 24 hours left sides
The right side, then plantlet of transplant to the pot for growing seedlings that matrix is housed is switched into normal management after shading 2-3 days.
Wherein, inducer described in step (2) includes following substance: described in inducer include following substance:
The dimethyl sulfoxide of 45ml/L, the colchicine of 4.5g/L, 100mg/L inositol, the heteroauxin of 25mg/L, 25mg/L 2,4-
D, the NAA of 25mg/L.
Described in step (2) repeatedly smearing until ovary surface can hang liquid.
The 10th day ovary expanded of Post flowering described in step (3) refers to that diameter is greater than 1 centimetre of ovary
Induced medium described in step (3), including MS+0.3mg/L6-BA+3%Suc+0.6%Agar+0.8mg/
LNAA+0.15mg/L active carbon, the percentage of culture medium be mass percent.
Alcohol described in step (3) is the alcohol of 75% volume ratio, soaking time 30-60s；The mercuric chloride solution
Mass concentration ratio be 0.1%, soaking time 8-10min；Aseptic water washing 3-5 times.
A kind of efficient selection of good quality and high output cotton provided by the invention is suitable for domestic upland cotton, island kind etc.
Kind.The material of cultivation can be directly used as breeding material, accelerate cotton breeding process.
The beneficial effects of the present invention are:
(1) the present invention provides a kind of method based on mutagens inducing cotton dihaploid, easy to operate, megaspore exists
It is doubled under the induction such as dimethyl sulfoxide, colchicine, the ovule that can normally expand is the successful euploid of induction.
(2) embryo expanded, by carrying out embryo rescue to the big embryo of Peng, improves luring for dihaploid in cotton since growing way is weak
Efficiency is led, the formation of later period blighted grain is avoided；Be added to the culture medium of proper proportion heteroauxin and gibberellin, make induce embryo at
Motility rate greatly improves.
(3) liquid is stained with by writing brush repeatedly to smear, avoid injury of the disposable a large amount of perfusion inducers to ovary, improve
Field operating efficiency and induction success rate；It protects bell agent and improves the survival rate for doubling rear embryo.
(4) hybrid vigour between sea island cotton and upland cotton can be quickly fixed, plan bottom solves sea Lu offspring's violent segregation
Problem.
Specific embodiment
Technical solution used in the embodiment of the present invention is the conventional scheme of this field if not otherwise specified；The reagent or
Material derives from commercial channel if not otherwise specified.
Embodiment 1:
A kind of efficient selection of good quality and high output cotton, includes the following steps:
1) upland cotton high yield strain 4133Bt is combined with the configuration of high-quality strain Hubei Province 0904, and F1 blooms the previous day to bud progress
Emasculation processing, and listing mark.
2) morning on the day of blooming is stained with dimethyl sulfoxide (DMSO) 45ml L with writing brush-1, colchicine 4.5g L-1, flesh
Alcohol 100mg L-1, heteroauxin 25mg L-1, 2,4-D 25mg L-1, 25mg/L NAA inducer, be applied to and remove repeatedly
In the ovary of stamen flower, style puts on ceratuba after smearing, prevents external source pollen, blooms second day and smears one time again.
3) Post flowering the 10th day, takes the ovary expanded, epidermis is cleaned up, with 75% alcohol on superclean bench
Solution impregnate after 60 seconds, then with 01% mercuric chloride solution immersion 8 minutes, 5 times after aseptic water washing, blot young fruit surface with aseptic paper
Moisture carefully separates ovule with the tip tweezers after sterilizing by young peach along ovary longitudinal incision；Ovule is inoculated in MS+0.3mg/
L6-BA (basic element of cell division)+3%Suc (sucrose)+0.6%Agar (agar)+0.8mg/LNAA (methyl α-naphthyl acetate)+0.15mg/L is living
Fiber differentiation is carried out in the induced medium of property charcoal, until bud is grown.
Embodiment 2:
A kind of efficient selection of good quality and high output cotton, includes the following steps:
1) high cotton yield strain 4133Bt is combined with high-quality 7124 configuration of strain sea of sea island cotton high yield, and F1 is right on the day before blooming
Bud carries out emasculation processing, and listing mark
2) morning on the day of blooming is stained with dimethyl sulfoxide (DMSO) 45ml L with writing brush-1, colchicine 4.5g L-1, flesh
Alcohol 100mg L-1, heteroauxin 25mg L-1, 2,4-D 25mg L-1, 25mg/L NAA inducer, be applied to and remove repeatedly
In the ovary of stamen flower, style puts on ceratuba after smearing, prevents external source pollen, blooms second day and smears one time again.
3) Post flowering the 5th day, takes the ovary expanded, epidermis is cleaned up, with 75% alcohol on superclean bench
Solution impregnate after 60 seconds, then with 01% mercuric chloride solution immersion 8 minutes, 5 times after aseptic water washing, blot young fruit surface with aseptic paper
Moisture carefully separates ovule with the tip tweezers after sterilizing by young peach along ovary longitudinal incision；Ovule is inoculated in MS+0.3mg/
L6-BA (basic element of cell division)+3%Suc (sucrose)+0.6%Agar (agar)+0.8mg/LNAA (methyl α-naphthyl acetate)+0.15mg/L is living
Fiber differentiation is carried out in the induced medium of property charcoal, until bud is grown.
Claims (6)
1. a kind of efficient selection of good quality and high output cotton, which comprises the following steps:
(1) cotton advantageous combination is configured；
(2) emasculation of F1 pollen is combined to cotton；
(3) ovary for removing stamen is smeared using inducer；
(4) 10 days ovarys expanded of Post flowering are chosen, rataria is taken out and is cultivated on MS culture medium, until after bud is grown；
(5) after seedlings root is suitable for, practice the transplanting of seedling matrix.
2. a kind of efficient selection of good quality and high output cotton according to claim 1, which is characterized in that specifically include
Following steps:
(1) the strong advantageous combination of configuration hybrid vigour, F1 generation carry out emasculation processing, and listed mark on the day before flowering stage is bloomed
Note；
(2) morning on the day of blooming is stained with inducer with writing brush, is applied in the ovary for removing stamen flower repeatedly, flower after smearing
Ceratuba on column sleeve prevents external source pollen, blooms second day and smears one time again, and Post flowering third day is smeared again protects bell agent；
(3) ovary expanded is taken in Post flowering the 10th day, carries out ovules culture in vitro, epidermis is cleaned up, in ultra-clean work
It is carried out disinfection again with mercuric chloride solution immersion after being impregnated on platform with alcoholic solution, after aseptic water washing, blots young fruit table with aseptic paper
Face moisture carefully separates ovule with tip tweezers by young peach along ovary longitudinal incision；Ovule is inoculated in induced medium and carries out
Fiber differentiation to bud is grown；
(4) when plant root development is suitable for, start hardening, first triangle bottle closure is opened, one layer of clear water is added and is placed in room temperature
Lower growth 6 hours, then takes out plant, cleans root culture medium, is put into triangular flask, pours into clear water and grows 24 hours or so,
Plantlet of transplant to the pot for growing seedlings that matrix is housed is switched into normal management after shading 2-3 days again.
3. a kind of efficient selection of good quality and high output cotton according to claim 1 or 2, it is characterised in that: described
Cotton advantageous combination is Lu Lu or land-sea combines.
4. a kind of efficient selection of good quality and high output cotton according to claim 2, which is characterized in that in step (2)
The inducer includes following substance: the dimethyl sulfoxide of 45ml/L, the colchicine of 4.5g/L, 100mg/L inositol,
The 2 of the heteroauxin of 25mg/L, 25mg/L, the NAA of 4-D, 25mg/L；Inducer is continuously smeared two days；Writing brush is stained with liquid and is applied repeatedly
It smears until capable of hanging liquid for ovary surface；Guarantor's bell agent includes following substance: the GA of 50mg/L3With the NAA of 100mg/L.
5. a kind of efficient selection of good quality and high output cotton according to claim 2, which is characterized in that in step (3)
The induced medium, including MS+0.3mg/L6-BA+3%Suc+0.6%Agar+0.8mg/LNAA+0.15mg/L activity
Charcoal, the percentage of culture medium be mass percent；The ovary expanded after blooming 10 days refers to that diameter is greater than 1 centimetre of son
Room.
6. a kind of efficient selection of good quality and high output cotton according to claim 2, which is characterized in that in step (3)
The alcohol is the alcohol of 75% volume ratio, soaking time 30-60s；The mass concentration ratio of the mercuric chloride solution is
0.1%, soaking time 8-10min；Aseptic water washing 3-5 times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811294949.1A CN109197580A (en) | 2018-11-01 | 2018-11-01 | A kind of efficient selection of good quality and high output cotton |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811294949.1A CN109197580A (en) | 2018-11-01 | 2018-11-01 | A kind of efficient selection of good quality and high output cotton |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110036906A (en) * | 2019-06-04 | 2019-07-23 | 中国农业科学院棉花研究所 | A method of irradiation pollen induction breeding good quality and high output cotton |
| CN111328704A (en) * | 2020-04-17 | 2020-06-26 | 中国农业科学院棉花研究所 | Method for inducing and breeding high-quality and high-yield cotton by utilizing Asian cotton pollen |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1219347A (en) * | 1997-12-12 | 1999-06-16 | 中国科学院遗传研究所 | Distant hybridization and breeding method for cotton |
| CN100998317A (en) * | 2006-11-13 | 2007-07-18 | 新疆农业科学院微生物应用研究所 | Technique for epicotyl of cotton culturing in vitro regenerated plant, and decapitated epicotyl explant |
| CN101120656A (en) * | 2007-09-21 | 2008-02-13 | 河北农业大学 | Culture medium and culture method for cotton immature embryo in vitro culture and plantlet formation |
| CN103931494A (en) * | 2014-03-17 | 2014-07-23 | 安徽农业大学 | Colored cotton ovule in vitro culture method |
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2018
- 2018-11-01 CN CN201811294949.1A patent/CN109197580A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1219347A (en) * | 1997-12-12 | 1999-06-16 | 中国科学院遗传研究所 | Distant hybridization and breeding method for cotton |
| CN100998317A (en) * | 2006-11-13 | 2007-07-18 | 新疆农业科学院微生物应用研究所 | Technique for epicotyl of cotton culturing in vitro regenerated plant, and decapitated epicotyl explant |
| CN101120656A (en) * | 2007-09-21 | 2008-02-13 | 河北农业大学 | Culture medium and culture method for cotton immature embryo in vitro culture and plantlet formation |
| CN103931494A (en) * | 2014-03-17 | 2014-07-23 | 安徽农业大学 | Colored cotton ovule in vitro culture method |
Non-Patent Citations (6)
| Title |
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| MANJEET SINGH GILL ET AL.: "Interspecific hybridization in the genus Gossypium through embryo culture", 《EUPHYTICA》 * |
| 宋平等: "活性炭培养基对棉花愈伤组织诱导的效果", 《植物生理学通讯》 * |
| 张献龙等: "陆地棉×亚洲棉胚胎在培养条件下的发育研究 ", 《华中农业大学学报》 * |
| 梁正兰等: "克服棉花种间杂交不亲和性及杂种F_1不育性的试验效果 ", 《遗传学报》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110036906A (en) * | 2019-06-04 | 2019-07-23 | 中国农业科学院棉花研究所 | A method of irradiation pollen induction breeding good quality and high output cotton |
| CN111328704A (en) * | 2020-04-17 | 2020-06-26 | 中国农业科学院棉花研究所 | Method for inducing and breeding high-quality and high-yield cotton by utilizing Asian cotton pollen |
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