CN105123495B - Application of partially indica-type paddy rice dominant dwarf mutant - Google Patents
Application of partially indica-type paddy rice dominant dwarf mutant Download PDFInfo
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- CN105123495B CN105123495B CN201510418415.5A CN201510418415A CN105123495B CN 105123495 B CN105123495 B CN 105123495B CN 201510418415 A CN201510418415 A CN 201510418415A CN 105123495 B CN105123495 B CN 105123495B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses an application of a partially indica-type paddy rice dominant dwarf mutant and belongs to the technical field of crop breeding. In the invention, natural mutation of genome of paddy rice, namely, a dominant dwarf mutant plant, is employed to obtain a stable and pure dominant dwarf mutant material through pollen tissue cultivation, thereby providing a dwarf source for breeding two-line or three-line hybridized paddy rice varieties. The selected dominant dwarf mutant can significantly reduce plant height of paddy rice, can improve anti-lodging performance and stable yield, and increases possibility of selecting an inter-subspecific hybridization variety and further provides substance conditions of increasing yield of the paddy rice and converting farming modes. With the stable and pure dominant dwarf material, a system being capable of randomly grouping and selecting the dwarf hybridization paddy rice varieties is established, by which the lodging problem, which often occurs during production, is solved. Meanwhile, the dominant dwarf mutant is suitable for a simplified cultivation mode in production of hybridized seeds and in large field, and can reduce labor intensity and production cost.
Description
Technical field
The invention belongs to rice breeding technology field, and in particular to a kind of indica-tendency type rice dominant dwarf mutant should
With.
Background technology
Plant height is the Main Agronomic Characters related to yield, according to the plant height of rice maturity, generally by Oryza sativa L. point
For high stalk, semi-dwarf mutant and three types of short stem, plant height easily causes lodging and the underproduction more than certain limit, of short stem to not only contribute to
It is resistant to lodging, and impoverishment tolerant, be conducive to improving yield.The breeding wheat for semidwarfness that late 1950s start, reduces Plant Height of Rice
To semi-dwarf mutant, rice yield obtains leap for the first time, becomes one of the significant achievement of " Green revolution ".Currently carry out
Super rice breeding be mainly further enhanced to rice yield using ideotype and subspecies indica and japonica hybrid advantage.
At present, long-grained nonglutinous rice breeding mostly utilizes sd-1 Semi dwarfism genes, and many in the breeding of short stem of Jing rice is also miscellaneous by Xian Jing
Hand over and import sd-1 genes, or using the short source of Jing rice with sd-1 genes.The Reusability of same gene, will cause improved variety
Hereditary basiies it is narrow, hereditary vulnerability will increase.And the dwarf gene utilized in rice breeding is all recessive gene, hybridization
In rice breeding, the use of Recessive dwarf genes often requires that parents must have same dwarf gene, which limits wider
Favourable parent is selected in general resource.But if utilizing indication law, then Rice Germplasm Resources can be made to obtain more effectively
Utilization and shorten breeding time.Therefore excavate new Plant Height of Rice control gene and be particularly indication law, to further
Improve rice yield significant.
The merits such as the nanism of dominant dwarf binding molecule marker assisted selection means, selection-breeding are made full use of to carry
The sterile line of dominant Dwarfing gene and restorer, innovate breeding material, to improveing the plant height of existing hybrid rice variety, improve
The anti-fall performance of hybrid rice variety.
The content of the invention
Technical problem
At present aborning can wide variety of Rice Dwarf major gene there was only sd-1, and other types of main base of short stem
Because or with bad gene close linkage, or with other bad character, such as grow thickly, thin stalk, dew section, granule etc., do not exist
Effectively utilizes are obtained in production, it is an object of the invention to provide indica-tendency type rice dominant dwarf, can make full use of its nanism
And other excellent economical characters, and binding molecule marker assisted selection means, selection-breeding carry the three of dominant Dwarfing gene and are or two
It is restorer and sterile line, to improveing the plant height of existing hybrid rice variety, improves the anti-fall performance of hybrid rice variety.
Technical scheme
A kind of application of indica-tendency type rice dominant dwarf mutant, it is characterised in that the indica-tendency type rice dominant dwarf is dashed forward
Variant is dominant short bar sd-y, and Oryza sativa L. (Oryzasativa), on May 7th, 2015 in Chinese microorganism strain preservation management committee
Member's meeting common micro-organisms center carries out preservation, and address Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences is protected
Tibetan number is CGMCC NO：10593.
Described dominant short bar sd-y for two can be or the cultivation of series of three-series hybrid rice kind provides the application in short source.With
Dominant short bar sd-y is male parent, with rice male restorer as male parent, improves the plant height of rice restorer.
Described dominant short bar sd-y can be in the application in the production of hybrid seeds or Production of Large Fields simple and laborsaving cultivation pattern or miscellaneous improving
It is applied in terms of handing over Oryza sativa L. lodging resistance kind.
Beneficial effect
The own Jing of lodging sex chromosome mosaicism of rice varieties becomes the important restriction factor of Rice Production and rice variety selective.Lodging
Yield of Rice Varieties decline is not only resulted in, but also causes deteriorating for rice quality.Affecting, the anti-fall property of rice varieties is relevant
Among factors, plant height is one of most important factor.
The indica type dominant dwarf sd-y of institute of the present invention selection-breeding started to show to downgrade (Fig. 1) from seedling stage, and tillering stage performance is short
Change and tiller increases (Fig. 2), be only 49.27cm to period of maturation sd-y plant height, less than the half of wild type plant height 98.47cm
(Fig. 3).In addition to Dwarfing phenotypes, also performance blade is dark green and tiller increases.When ripe, tiller is about 1.4 times of wild type.With
Wild type is compared, and each internodes of sd-y averagely shorten, and is the mutant of dn types.
With the indica type dominant dwarf sd-y of institute of the present invention selection-breeding as maternal (plant height 49.27cm), with Dular,
Kasalath, Kui rice, KN configure the plant height about 100cm of cross combination for male parent, and plant height reduces amplitude about 50%.
The present invention is entered from different parent materials using the japonica rice line for carrying dominant dwarf rice mutant gene
Row artificial hybridization, can effectively reduce the plant height of the hybrid rice of institute's selection-breeding, improve the lodging resistance of the hybrid rice of institute's selection-breeding, be solution
The new resources approach of the super close problem exploration of plant height certainly during rice varieties and subspecies high yield and Breeding For Super High-yielding.
Description of the drawings
Plant phenotypes of Fig. 1 sd-y at seedling stage
Plant phenotypes of Fig. 2 sd-y at heading stage
Fig. 3 sd-y and plant phenotype of the wild type at heading stage
Fig. 4 sd-y and high stalk parent Dular and its F1Plant height at heading stage
Biological deposits
Dominant short bar sd-y, Oryza sativa L. (Oryzasativa) were managed in Chinese microorganism strain preservation on May 7th, 2015
Committee's common micro-organisms center carries out preservation, address Yard 1, BeiChen xi Road, Chaoyang District, Beijing City institute of microbiology of the Chinese Academy of Sciences,
Preserving number is CGMCC NO：10593.
Specific embodiment
A kind of selection of quick acquisition indica-tendency type rice dominant dwarf mutant sd-y of the present invention, including following
Step：
In the rice breeding F of Jiangsu Inst. of Coastal Area Agricultural Sciences's experimental plot plantation2For selective nursery, (numbering is
F2009, it is combined as extensive 269) the middle acquisition indica type Dwarf Mutants material (plant height 50cm or so) of Weedy Rice × salt；Self progeny is high
Bar and serious separation of short stem, choose the individual plant consistent with phenotype with mutant plant height, and continuation plantation of reserving seed for planting, using the M of improvement8Training
Foster base carries out pollen tissue culture.
Pollen tissue culture
Inducing culture is used for the minimal medium of callus induction and is M8Additional 30g L in culture medium, and culture medium-1Sucrose, 8g L-1Agar powder and 2mg L-12,4-D (2,4- phenoxy acetic acids).Pyrusussuriensiss are addition of in inducing culture also
Alcohol 5g L-1, and 0.2mg L-1KT (kinetins) and 1mg L-1The exogenous hormones such as NAA (naphthalene acetic acid).
It is with M that division culture medium is used for the culture medium of long-grained nonglutinous rice differentiation8For minimal medium, sucrose concentration is 30g L-1With
Glucose 30g L-1, agar powder 8g L-1, 1g L-1Activated carbon, additional 2mg L are set-16-BA and 1mg L-1KT。
Root media is that, with MS as minimal medium, sucrose concentration is 15g L-1, agar powder 8g L-1, 1mg L-1IAA, 2mg L-1PPP (paclobutrazol) 4mg L-1Colchicine.
Pretreatment
Take fringe is typically carried out the fine day dusk time in, is chosen pollen differentiation and is in the tassel of mid-late uninucleate stage, pulvinus away from
Long 10-15cm or so.Spike of rice is wrapped by 75 alcohol wipes of surface with gauze, is put into refrigerator, 6-10 DEG C of Cold pretreatment
3 days.
The induction of Rice anther culture calluss
By the tassel fetched, 75% Ethanol Treatment 90S of Jing, 15min is soaked with 10% hydrogen peroxide solution, then floated with sterilized water
Wash 3-4 time, then the tassel sterilized grain husk shell is cut off, pollen is inoculated in above inducing culture.Per bottle of 100 pieces of inoculation is left
The right side, each material do 10 bottles, are put under light culture room (26 ± 2) DEG C dark condition and cultivate.
The differentiation of Rice Callus and plant regeneration
The calluss of well-grown, compact structure are selected, division culture medium is gone to, each culture dish connects 5-6 wound healing
Tissue, cultivates under (26 ± 2) DEG C illumination condition, and light intensity is 400 μm of ol m-2·s-1, daily illumination 16h, 30d is left for culture
The right side, evoking adventive bud, and the tissue for producing green bud is gone in the strong seedling culture base with MS as minimal medium, (26 ± 2) DEG C
Cultivate under illumination condition so as to take root and grow up to whole plant.
Transplant land for growing field crops
Treat that green Seedling length to 15cm or so, has a plurality of adventitious root time of origin, removes it test tube, be placed on laboratory room temperature
Under carry out seedling exercising, transplant after 3 days to big field, obtain indica-tendency type rice dominant dwarf mutant sd-y.
Sd-y started to show to downgrade (Fig. 1) from seedling stage, and tillering stage performance is downgraded and tiller increases (Fig. 2), to the period of maturation
Sd-y plant heights are only 49.27cm, less than the half (Fig. 3) of wild type plant height 98.47cm.In addition to Dwarfing phenotypes, blade is also showed
Dark green and tiller increases.When ripe, tiller is about 2 times of wild type.Compared with wild type, each internodes of sd-y averagely shorten, and are
The mutant of dn types.Indica-tendency type rice dominant dwarf mutant sd-y, other economical characters are shown in (table 1).
Indica type dominant dwarf sd-y and wild type (it is public, see national Oryza sativa L. data center http://
Www.ricedata.cn/index.htm) reciprocal crossess F1Expression mutation body surface type, shows that short strain is dominant to high strain completely.sd-
Y Inheritance Analysis on Genetic：Winter in 2012 plants the F of cross combination sd-y and Dular in Hainan2Colony, in F2In segregating population altogether
The individual plant height of 575 plants of investigation, using 75cm as the demarcation line with high stalk of short stem, as a result shows that stalk strain number of short stem, high is respectively 421
With 154 plants, meet 1 showed to gene 3：1 segregation ratio (χ2=1.81<χ20.05=3.84), show that sd-y mutant phenotypes are
By a pair dominant karyogene controls.
The indica dominant dwarf sd-y (plant height 49.27cm) with institute's selection-breeding referring to (table 2), with Dular,
Kasalath, Kui rice, KN (notes：It is public, sees national Oryza sativa L. data center http://www.ricedata.cn/
Index.htm) positive and negative hybridization configuration combination, offspring F1Plant height about 100cm, plant height reduce amplitude about 50%.
1 dominant dwarf sd-y of table is compared with wild type economical character
2 indica type dominant dwarf sd-y of table configures cross combination plant height table
Table 3 improves M8Culture medium prescription
Claims (6)
1. a kind of application of indica-tendency type rice dominant dwarf mutant in terms of rice breeding, it is characterised in that the indica water
Rice dominant dwarf mutant is dominant short bar sd-y, and Oryza sativa L. (Oryza sativa), on May 7th, 2015 in China Microbiological
Culture presevation administration committee common micro-organisms center carries out preservation, the address Yard 1, BeiChen xi Road, Chaoyang District, Beijing City Chinese Academy of Sciences
Institute of microbiology, preserving number are CGMCC NO：10593.
2. application according to claim 1, it is characterised in that the application refers to the dominant short bar described in claim 1
Sd-y for two is or the cultivation of series of three-series hybrid rice kind provides the application in short source.
3. application according to claim 1 and 2, it is characterised in that the application referred to dominant short bar sd-y as male parent,
With rice male restorer as male parent, the plant height of rice restorer is improved.
4. application according to claim 1 and 2, it is characterised in that the application refers to that described dominant short bar sd-y exists
Application in the production of hybrid seeds or Production of Large Fields simple and laborsaving cultivation pattern.
5. application according to claim 1 and 2, it is characterised in that the application refers to that described dominant short bar sd-y exists
Application in terms of raising hybrid rice lodging resistance kind.
6. application according to claim 3, it is characterised in that the application refers to the dominant short bar described in claim 1
Applications of the sd-y in terms of hybrid rice variety lodging resistance is improved.
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184651B (en) * | 2018-01-16 | 2021-05-07 | 江西红一种业科技股份有限公司 | Lodging-resistant rice variety breeding method |
| CN113273460B (en) * | 2021-06-17 | 2023-05-02 | 宁波市农业科学研究院 | Lodging-resistant conventional japonica rice breeding method |
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| EP1275719A4 (en) * | 2000-03-31 | 2007-04-25 | Nat Inst Of Agrobio Sciences | Gene concerning brassinosteroid-sensitivity of plants and utilization thereof |
| WO2007046403A1 (en) * | 2005-10-19 | 2007-04-26 | National University Corporation Nagoya University | Plant belonging to genus oryza having dominant dwarf trait, and use thereof |
| CN101701221A (en) * | 2009-11-25 | 2010-05-05 | 天津师范大学 | Rice dwarf straw leaf roll mutant (cd1) gene and application thereof |
| CN101747420A (en) * | 2009-12-29 | 2010-06-23 | 中国科学院遗传与发育生物学研究所 | Dominant dwarf rice related protein, encoding gene thereof and application |
| WO2010076766A1 (en) * | 2008-12-30 | 2010-07-08 | Institute Of Genetics And Developmental Biology | Genes associated with plant tiller number and uses thereof |
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| EP1275719A4 (en) * | 2000-03-31 | 2007-04-25 | Nat Inst Of Agrobio Sciences | Gene concerning brassinosteroid-sensitivity of plants and utilization thereof |
| WO2007046403A1 (en) * | 2005-10-19 | 2007-04-26 | National University Corporation Nagoya University | Plant belonging to genus oryza having dominant dwarf trait, and use thereof |
| WO2010076766A1 (en) * | 2008-12-30 | 2010-07-08 | Institute Of Genetics And Developmental Biology | Genes associated with plant tiller number and uses thereof |
| CN101701221A (en) * | 2009-11-25 | 2010-05-05 | 天津师范大学 | Rice dwarf straw leaf roll mutant (cd1) gene and application thereof |
| CN101747420A (en) * | 2009-12-29 | 2010-06-23 | 中国科学院遗传与发育生物学研究所 | Dominant dwarf rice related protein, encoding gene thereof and application |
| CN102559653A (en) * | 2011-01-02 | 2012-07-11 | 中国科学院遗传与发育生物学研究所 | Application of OsDCL3b in dwarf rice cultivation |
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