CN1663356A - Transferring and seed producing method for head cabbage cytoplasm male sterile line - Google Patents
Transferring and seed producing method for head cabbage cytoplasm male sterile line Download PDFInfo
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Abstract
The invention discloses a transferring method for head cabbage cytoplasm male sterile line characterized by that, wild cabbage self-bred lines are utilized for 5-7 generation backcrossing with cabbage cytoplasmic protein sterility source. The advantages of the invention include stabilized male sterility, high sterility strain rate and sterility degree. The crossing rate of the obtained hybrid in strictly isolated area can be as high as 100%.
Description
Technical field
The present invention relates to plant heterosis utilization field, relate in particular to transformation of a kind of wild cabbage cytoplasmic male sterile line and producing method for seed.
Background technology
Wild cabbage (Brassica oleracea var.capitata (L.)) is the main vegetables of countries in the world, because its hybrid vigour is obvious, heterosis breeding becomes the main path of wild cabbage breeding.As far back as 50～sixties of eighties of last century, developed countries such as Japan, the U.S. just utilize self incompatible line approach preparation cabbage hybrid, and China also utilizes the self incompatible line method to produce cabbage hybrid since the seventies.But this method exists, and the hybrid rate of crossbreed is difficult to reach 100%, the long-term self propagated of parent of crossbreed is prone to that vitality is degenerated, the parent is by problems such as artificial flower bud phase pollination self reproductive-cost height, and utilizes the male sterile line production of hybrid seeds can overcome above-mentioned defective.Therefore, for a long time wild cabbage breeding work person all in the research of actively carrying out the wild cabbage male-sterile line breeding.
There are nuclear gene male sterility and cytoplasmic male sterility two big classes in wild cabbage male sterile source.Nuclear gene male sterility has two kinds of recessive nuclear gene and dominance nuclear genes again, and the recessive nuclear gene male sterile line is difficult to utilize because of finding maintenance line.For the dominance genetic male sterile line, cultivated success and be used in the production of hybrid seeds, but the transformation process to be complicated, the dominance male sterile plant that isozygotys that is used to breed male sterile line will expand numerous with the method for tissue culture.The cytoplasmic male sterilty material has radish kytoplasm and black mustard kytoplasm two classes, and black mustard cytoplasmic male sterility has been proved and can not have used.The radish kytoplasm wild cabbage male sterile line major part that more domestic units had collected is because of the plant strain growth and the undesired and very difficult utilization of proterties of blossoming and bearing fruit.The improvement radish cytoplasm wild cabbage male sterile line of having collected since the mid-90 in last century, though plant strain growth with blossom and bear fruit normally, economical character is bad, regularity is poor, can not directly utilize.Therefore, but the seed selection sterility is stable, transformation easily the wild cabbage cytoplasmic sterile line of practical application be wild cabbage breeding work person hope for many years.
Summary of the invention
Goal of the invention of the present invention is that transformation goes out that male sterility is stable, plant and the proterties of blossoming and bearing fruit is normal, coordinate force is good wild cabbage cytoplasm male sterility line, utilize the good hybridization between selfed lines of cytoplasm male sterility line and coordinate force again, make good cabbage hybrid, and set up the production of hybrid seeds new method of utilizing wild cabbage cytoplasm male sterility line preparation cabbage hybrid.
The invention provides a kind of transfer method of wild cabbage cytoplasmic male sterile line, it is characterized in that, is that 5-7 is carried out for backcrossing in recurrent parent and wild cabbage cytoplasmic sterility source with the wild cabbage selfing.
Wherein said wild cabbage cytoplasmic sterility source is CMS9825 or CMS9829; 98403,98408,98432,98G73,98G79,9866,9880,98101 etc. described wild cabbage self-compatible is:.
The present invention also provides a kind of producing method for seed of wild cabbage cytoplasmic male sterile line; it is characterized in that; the wild cabbage cytoplasmic male sterile line of the transfer method gained that use is above-mentioned and the seed of corresponding wild cabbage inbred line are in planted in fall; time of the year when autumn changes into winter selects kind of strain in the vernalization of surviving the winter with protecting; plant male sterile line and wild cabbage inbred line in an Isolation warm house or net canopy together 1 year spring; male sterile line and wild cabbage inbred line plant ratio are 2: 1 or 3: 1; before kind of a strain is bloomed, put honeybee into and be used for pollination; behind the seed maturity, be the wild cabbage cytoplasmic male sterile line seed that the preparing hybrid kind is used from the sterile seed of fastening results.
In addition, the present invention also provides a kind of production method of cabbage hybrid, it is characterized in that, uses the wild cabbage cytoplasmic male sterile line of above-mentioned transfer method gained to be female parent, and wild cabbage self-compatible is that male parent is hybridized the acquisition crossbreed.
Entity of the present invention is to make male parent with good cytoplasmic sterility source work female parent, good inbred line, the transformation cytoplasm male sterility line that proterties is normal, coordinate force is good that becomes to grow and to blossom and bear fruit, and utilize cytoplasmic male sterile line to make good cabbage hybrid, the present invention has the following advantages: male sterility is controlled by cytoplasm, transformation is easy, and any wild cabbage inbred line all can be made its maintenance line; Male sterility is stable, and sterile plant rate and sterile degree all can reach 100%, and are not subjected to environmental influence, and the crossbreed hybrid rate with its preparation in strict isolated area can reach 100%; The proterties and coordinate force is all very normal, nectary is flourishing of growing, blossom and bear fruit of male sterile line plant is better than the radish kytoplasm wild cabbage male sterile line of black mustard kytoplasm and unmodified; The all available self compatible line of the maintenance line of male sterile line and the male parent of crossbreed can be with the honeybee pollination breeding under the isolation condition, and cost is low.
The quality in male sterile source is the key that can the cytoplasm male sterility line that selects of decision practical application, a good cytoplasmic sterility source should possess following good characteristic: (1) male sterility is stable, and the sterility performance is not subjected to the influence of changes in environmental conditions; (2) the male sterility transformation is easy, finds maintenance line easily; (3) vine growth and development is normal, staminody, but floral organ such as gynoecium, nectary and blossom and bear fruit normal.
The later stage eighties of last century later stage seventies to the nineties, successively collected black mustard cytoplasmic male sterilty material C MS79091, wild cabbage cytoplasmic male sterilty materials such as radish cytoplasm wild cabbage male sterile material CMS409, CMS9511, CMS9825, CMS9829, adopt following several methods it is grown and blossoms and bears fruit proterties to identify: (1) identifies their plant strain growth speed and main economic characters under normal field management condition; (2) identify that under the cryogenic conditions about 15 ℃ each male sterile source plant leaf look changes and growth rate; (3) with sterile be that maternal, dissimilar selfings are that male parent is carried out test cross and backcrossed, study its offspring's sterility performance; (4), investigate their situation of blooming and the growing state of floral organ and kind pod in flowering stage and pod bearing period.
Result of the test shows, the growth of black mustard kytoplasm wild cabbage male sterile material CMS79091 sterile plant is normal, plant leaf look and growth rate are normal under the cryogenic conditions, but among the test cross offspring of sterile strain, sterile plant rate only reaches between 33.7～60%, and most sterile flower is semi-open state, can not open, nectary is little or do not have, and influence solid under insect pollination and the open pollination condition, so this male sterile source is difficult to utilization.
The male sterility of several parts of radish kytoplasm wild cabbage male sterile materials such as CMS409, CMS9511, CMS9825, CMS9829 is all very stable, and sterile plant rate and sterile degree are 100%, and all wild cabbage inbred lines all are their maintenance lines.But not only plant leaf yellow under 15 ℃ of left and right sides cryogenic conditions of CMS409, plant strain growth speed are slow, and the proterties of blossoming and bearing fruit is undesired, and most flowers can not open, part gynoecium deformity, little or the nothing of nectary, about 50% kind pod deformity can't be utilized in the wild cabbage hybrid seeding.Though its plant of CMS9511 not yellow of blade at low temperatures, but the plant strain growth and the proterties of blossoming and bearing fruit remain in many problems: how to be slower than corresponding maintenance line for the back male sterile line plant strain growth speed of backcrossing, most flowers are semi-open states, many flower gynoeciums are undesired, nectary is undeveloped, and 40% kind of pod deformity is arranged approximately.Therefore this cytoplasmic male sterilty source also is difficult in the wild cabbage hybrid seeding and utilizes.
Find through test for many years, two parts of cytoplasmic male sterilty materials of CMS9825, CMS9829 remove not yellow of plant leaf at low temperatures, plant strain growth speed normally outside, the proterties of blossoming and bearing fruit is also normal, most sterile flowers can open, and gynoecium is almost all normal, the nectary prosperity, on the wild cabbage hybrid seeding good application prospects being arranged, is two parts of good wild cabbage cytoplasmic male sterilty sources (seeing Table 1).
New good male sterile line all needs to make male parent with inbred line and through how could obtain for the continuous backcross transformation.Inbred line as continuous backcross is exactly the maintenance line of male sterile line, and the quality of maintenance line (male parent of backcrossing inbred line) has determined the quality of transformation success back each proterties of male sterile line.As a good maintenance line, should possess following 4 characteristics: (1) principal character characteristic good, stable and consistent; (2) good coordinate force is arranged; (3) backcross transformation offspring male sterile plant rate and sterile degree all reach 100%; (4) florescence pollination self performance is affine, though or the florescence selfing not affine, flower bud phase self-compatible index can reach more than 10.
The seed selection of maintenance line is mainly by continuous backcross, the directed method of selecting.Make starting material with good germ plasm resource, pursue generation and select the continuous selfing of fine individual plant to identify that select time is generally 6 generations or longer till each objective trait is stable.The part good maintenance line of table 2 for filtering out in recent years.
Though CMS9825, the not yellow at low temperatures of two parts of wild cabbage cytoplasmic sterilities of CMS9829 material plant, growth rate and blossom and bear fruit normal, but plant strain growth state, leaf-head size are irregular, it is not strong to plant disease resistance autumn, plant center column length (table 1) in the ball spring, illustrate that the economic characters of these two parts of materials are not good enough.What is more important, these two parts of materials are half-blood, can not directly utilize.
Male sterility is stable in order to obtain, vine growth and development and normal, the economic characters good stable of blossoming and bearing fruit, the good cytoplasmic male sterilty of coordinate force, the present invention has taked the method for continuous backcross transformation, make maternal (sterile source) with the CMS9825, the CMS9829 that filter out, select proterties is stable, coordinate force is good dissimilar height to carry out the continuous backcross transformation for male parent for inbred line 98403,98408,98432,98G73,9880,9866,98G79,98101 etc.In the transformation process, observe its offspring's male sterility and blossom and bear fruit proterties and economic characters performance, and make orientation and select, the standard of maternal strain of select continuing to backcross is: (1) male sterility is stablized, flower pesticide degeneration WUHUAFEN, and sterility is not subjected to the influence of changes in environmental conditions; (2) flower bud development is normal, and not dead flower bud or dead flower bud are few, and flower is open fully, and gynoecium is normal, the nectary prosperity, and nectar is many, and the pollination back is solid good, plants pod and grows normal; (3) vine growth and development is normal, the not yellow of low temperature inferior lobe look, and the male parent of backcrossing is inclined to or is similar to main economic characters.
Carry out transformation with said method, its backcross progeny male sterility is stable, and all wild cabbage inbred lines all can be made its maintenance line, general with after 5～7 generations of fixing stable inbred line transformation, just can obtain the economic characters wild cabbage cytoplasm male sterility line similar with the male parent of backcrossing, and the male parent of backcrossing is exactly its maintenance line to coordinate force.But obtained the wild cabbage cytoplasm male sterility line (table 3) of a collection of practical application at present.
With the seed of cytoplasm male sterility line and corresponding maintenance line in planted in fall; time of the year when autumn changes into winter selects kind of strain in the vernalization of surviving the winter with protecting; plant male sterile line and maintenance line in an Isolation warm house or net canopy together 1 year spring, and male sterile line and maintenance line plant ratio were generally 2: 1 or 3: 1.Because maintenance line more options self compatible line, in the net canopy, to put honeybee in preceding about 7 days into and be used for pollinating so can bloom in kind of a strain, area is that the net canopy about a mu should be put 2～3 casees honeybees.Behind the seed maturity, be the cytoplasm male sterility line seed that the preparing hybrid kind is used from the sterile seed of fastening results.
After good cytoplasm male sterility line had been arranged, the key of matching out good cytoplasm male sterility line cabbage hybrid was the male parent that chooses crossbreed, and it should possess following condition: (1) main economic characters are good, neat, self-compatible; (2) coordinate force is good, and is far away with the male sterile line affiliation, and hybridization back hybrid vigour is strong; (3) bloom normal, pollen amount is big, florescence and male sterile line basically identical.
Cytoplasm male sterility line with the transformation success is maternal, is male parent with the wild cabbage selfing that meets above-mentioned condition, has made a collection of good hybrid combination (table 4).Wherein 02-416 is an autumn cabbage hybrid that precocious high-quality is disease-resistant, autumn is when planting, viral diseases and black rot, more heat-resisting, newel is short in leaf-head consolidation, nearly spheroidal, anti-blastomere, the ball, and field planting is to gathering in the crops 5047.3 kilograms of per mu yields about 60 days, sweet No. 8 precocities are 7～10 days in the comparison photograph kind, volume increase 13.2～15.2%.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.
Embodiment 1:
With 9866 is that backcross parent and CMS9825 carry out 5 generations backcross transformation acquisition wild cabbage cytoplasmic male sterile line CMS9866.With CMS9866 is that female parent, wild cabbage self compatible line 8180 are male parent preparing hybrid kind 02-416.
Embodiment 2:
With 98432 is that backcross parent and CMS9829 carry out 6 generations backcross transformation acquisition wild cabbage cytoplasmic male sterile line CMS98432.With CMS98432 is that female parent, wild cabbage self compatible line 91-276 are male parent preparing hybrid kind 03-33.
Embodiment 3:
With 98408 is that backcross parent and CMS9829 carry out 5 generations backcross transformation acquisition wild cabbage cytoplasmic male sterile line CMS98408.With CMS98408 is that female parent, wild cabbage self compatible line 96-100 are male parent preparing hybrid kind 03-52.
Embodiment 4:
With 9880 is that backcross parent and CMS9825 carry out 7 generations backcross transformation acquisition wild cabbage cytoplasmic male sterile line CMS9880.With CMS9880 is that female parent, wild cabbage self compatible line 7014 are male parent preparing hybrid kind 03-31.
Produce cabbage hybrid with cytoplasm male sterility line, similar to usefulness self incompatible line production of hybrid seeds some places on seeding technique, but many places are inequality, its main points are as follows:
(1), cultivated kind of the strain of collecting seed before the winter: begin sowing in good time sooner or later in the maturing stage by the parent, late variety was sowed in early August, and early-maturing variety was sowed in mid or late August.The plot that Seedlings nursery should be selected higher, the easy row of physical features, pour water, after planting about 2～3 true leaf time-division seedlings once, field planting during 7～8 leaves is planted the strain field growing phase and is wanted timely prevention and elimination of disease and pests.
(2), pay attention to kind of a strain safe overwintering vernalization
The kind strain of middle part, North China is heeled in before the winter in cold bed or small arched shed and is survived the winter, and southern areas can directly be colonizated in open country and survive the winter.Be frost damage prevention, the very night, the kind strain of surviving the winter on protection ground should hide Polypropylence Sheet and straw screen or mat evening during temperature drop to 0～-4 ℃, and will in time take off lid daytime.The kind strain that open country is survived the winter can be in kind of a strain base portion ridging.
(3), in good time field planting kind strain in spring
Planting strain can be in the late March field planting through winter low temperature vernalization, and physical features is smooth, the plot of the fertile irrigation and drainage in soil but production of hybrid seeds ground should be selected.Density is about every mu 4000 strain, and maternal ratio with male parent is 2: 1 or 3: 1.
(4), guarantee the honeybee source of distance isolation distance and pollination
Wild cabbage is a cross pollinated plant, easy and brassica crop hybridization, and seed farm should be isolated more than 1500 meters with other brassica crop seed farm.Wild cabbage is an entomophilous flower, makes maternal preparing hybrid kind with male sterile line, by honeybee the pollen of male parent is passed on the maternal column cap entirely during hybridization, and for guaranteeing pollination fully, every mu of seed farm is put honeybee about 2 the casees at least.
(5), the strict purity of noting the parent
In each link of the production of hybrid seeds, comprise just seedling stage, rosette stage and flowering stage, note parent's roguing.
(6), regulate parents' florescence unanimity, in time pull out the strain of male parent kind, gather in the crops seed in good time
If parents are inconsistent flowering stage, the date of seeding of should taking to stagger, pinch, measure such as training regulates parents' florescence consistent as far as possible.After parents' florescence finishes, should in time the strain of male parent kind all be pulled out anti-kind of strain lodging of built simultaneously.When kind of pod yellow maturity, in time gather in the crops the seed on the female parent, kind strain after the results and the seed of laying are wanted timely airing, influence seed germination rate in order to avoid go mouldy.
Table 1, the main economic characters of CMS9825, CMS9829
| The cytoplasmic sterility title material | Regularity | Plant degree of development (cm) | The outer number of sheets (sheet) | Lobus cardiacus sheet color under the low temperature | Field planting is to results fate (d) | The leaf-head sphere | Single ball heavy (kg) | Sphere diameter (wide * height) (cm) | Center column length (cm) | Male sterility % | Nectary | Deformity gynoecium % | Deformity is planted pod % | Dead bud situation |
| ??CMS ??9825 | Medium | ??42-48 | ??12-15 | Grayish green | ??60 | High circle or nearly circle | ????0.5-0.7 | ??13.5 ??× ????14.5 | ??8.0-9.5 | ??100 | In to big | ??4.5 | ??5.5 | Medium |
| ??CMS ??9829 | Medium | ??52-59 | ??13-16 | Dark green | ??65 | Circle or nearly circle | ????0.6-0.9 | ??15.0 ??× ????14.5 | ??8.5-9.5 | ??100 | In to big | ??4.6 | ??6.5 | Medium |
The main economic characters of table 2, the good maintenance line of part
| The maintenance line title | Plant degree of development (cm) | The outer number of sheets (sheet) | The leaf look | Field planting is to results fate (d) | The leaf-head sphere | Single ball heavy (kg) | Sphere diameter (wide * height) (cm) | Center column length (cm) | Disease resistance | Be suitable for cultivation season |
| ??98408 | ??43.7× ????42.0 | ??13.3 | Green | ??45 | Ball | 1.10 | ????14.0× ?????????15.2 | ????6.5 | In anti- | Extremely early mature, spring and autumn dual-purpose |
| ??98432 | ??34.8× ????34.0 | ??10.0 | Grayish green | ??45 | OX-heart | 0.78 | ????13.3× ?????????14.0 | ????6.8 | In anti- | The spring extremely early mature |
| ??98G73 | ??39.7× ????37.0 | ??12.3 | Dark green | ??50 | Ball | 0.95 | ????13.3× ?????????14.2 | ????7.0 | In anti- | The spring precocity |
| ????9880 | 45.0× 44.2 | ??13.5 | Grayish green | ????75 | Oblate | 1.40 | 16.8× 13.5 | ????7.0 | Anti- | Ripe in autumn |
| ????9866 | 50.0× 51.8 | ??14.2 | Grayish green | ????75 | Oblate | 1.65 | 19.5× 12.8 | ????6.2 | Anti- | In ripe, spring and autumn dual-purpose |
| ????98G79 | 42.0× 43.3 | ??13.0 | Green | ????50 | Gao Yuan | 0.93 | 3.8× 15.3 | ????7.0 | In anti- | The spring precocity |
| ????98403 | 48.7× 49.0 | ??14.3 | Green | ????55 | Gao Yuan | 1.01 | 4.3× 18.3 | ????8.0 | In anti- | The spring mid-early maturity |
| ????98101 | 48.0× 49.0 | ??12.5 | Dark green | ????60 | OX-heart | 1.50 | 15.2× 19.5 | ????9.0 | In anti- | The spring mid-early maturity |
The main plant proterties and the economic characters of table 3, the good wild cabbage cytoplasm male sterility line of part
| The cytoplasm male sterility line title | Plant degree of development (cm) | The outer number of sheets (sheet) | The leaf look | Field planting is to results fate (d) | The leaf-head sphere | Single ball heavy (kg) | Sphere diameter (wide * height) (cm) | Center column length (cm) | Nectary | Deformity gynoecium % | Male sterile plant rate % | Be suitable for the preparing hybrid composite type |
| CMS 98403 | ??50.1× ??????51.0 | ??13.5 | Green | 55 | Gao Yuan | 1.10 | ?14.5× ????18.5 | ????8.5 | In or big | ＜5 | ?100 | The spring mid-early maturity |
| CMS 98408 | ??45.2× ??????44.3 | ??14.0 | Green | 45 | Ball | 1.21 | ?14.5× ????15.8 | ????6.8 | In or big | ＜5 | ?100 | The spring extremely early mature |
| CMS 98432 | ??35.2× ??????34.8 | ??11.2 | Grayish green | 45 | OX-heart | 0.85 | ?13.8× ????14.2 | ????6.9 | In or big | ＜5 | ?100 | The spring extremely early mature |
| CMS 98G73 | ??40.2× ??????41.0 | ??13.1 | Dark green | 50 | Ball | 1.05 | ?13.5× ????14.5 | ????7.2 | In or big | ＜5 | ?100 | The spring precocity |
| ??CMS ??9880 | ??45.2× ????45.3 | ??13.8 | Grayish green | ??75 | Oblate | 1.45 | ?16.8× ??14.2 | ????7.1 | In or big | ＜5 | ?100 | Ripe in autumn |
| ??CMS ??98101 | ??50.0× ????49.2 | ??13.0 | Dark green | ??60 | OX-heart | 1.52 | ?15.0× ??18.5 | ????9.0 | In or big | ＜5 | ?100 | The spring mid-early maturity |
| ??CMS ??9866 | ??53.8× ????54.4 | ??14.2 | Grayish green | ??75 | Oblate | 1.68 | ?23.2× ??13.8 | ????6.0 | In or big | ＜5 | ?100 | Ripe in the spring and autumn dual-purpose |
| ??CMS ??98G79 | ??43.2× ????43.8 | ??13.5 | Green | ??50 | Gao Yuan | 1.05 | ?13.5× ??15.8 | ????7.2 | In or big | ＜5 | ?100 | The spring precocity |
Table 4, several good hybrid combination with the cytoplasm male sterility line preparation
| Field number | Hybrid combination | Plant degree of development (cm) | The outer number of sheets (sheet) | The leaf look | Field planting is to results fate (d) | Spherical | Sphere diameter (wide * height) (cm) | Center column length (cm) | Single ball heavy (kg) | Be suitable for cultivation season |
| 02-416 | ?CMS9866 ?×8180 | ??59.5× ????60.3 | ??14.1 | Dark green | ??60 | Nearly circle | 17.5×16.5 | ??7.0 | ????1.90 | Autumn mid-early maturity |
| 03-33 | ?CMS98432 ?×91-276 | ??43.0× ????44.6 | ??10.2 | Dark green | ??50 | Nearly circle | 15.3×16.5 | ??6.7 | ????1.25 | Spring precocity |
| 03-52 | ?CMS98-408 ?×96-100 | ??55.0× ????53.2 | ??13.2 | Dark green | ??50 | Gao Yuan | 14.2×15.5 | ??5.5 | ????1.10 | Autumn precocity |
| 03-31 | ?CMS9880 ?×7014 | ??65.2× ????65.5 | ??14.0 | Grayish green | ??80 | Oblate | 26.0×14.8 | ??7.2 | ????2.90 | Ripe in autumn |
Claims (5)
1, a kind of transfer method of wild cabbage cytoplasmic male sterile line is characterized in that, is that 5-7 is carried out for backcrossing in recurrent parent and wild cabbage cytoplasmic male sterilty source with the wild cabbage selfing.
2, transfer method as claimed in claim 1 is characterized in that, described wild cabbage cytoplasmic sterility source is CMS9825 or CMS9829.
98403,98408,98432,98G73,98G79,9866,9880,98,101 3, transfer method as claimed in claim 1 is characterized in that, described wild cabbage self-compatible is:.
4; a kind of producing method for seed of wild cabbage cytoplasmic male sterile line; it is characterized in that; to use the seed of the wild cabbage cytoplasmic male sterile line of transfer method gained as claimed in claim 1 and corresponding wild cabbage inbred line in planted in fall; time of the year when autumn changes into winter selects kind of strain in the vernalization of surviving the winter with protecting; plant male sterile line and wild cabbage inbred line in an Isolation warm house or net canopy together 1 year spring; male sterile line and wild cabbage inbred line plant ratio are 2: 1 or 3: 1; before kind of a strain is bloomed, put honeybee into and be used for pollination; behind the seed maturity, be the wild cabbage cytoplasmic male sterile line seed that the preparing hybrid kind is used from the sterile seed of fastening results.
5, a kind of production method of cabbage hybrid is characterized in that, uses the wild cabbage cytoplasmic male sterile line of transfer method gained as claimed in claim 1 to be female parent, and wild cabbage self-compatible is that male parent is hybridized.
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| CN104206150B (en) * | 2014-08-25 | 2016-05-11 | 镇江瑞繁农艺有限公司 | A kind of method that improves cabbage sterile lines seed making output |
| CN106171948A (en) * | 2016-07-06 | 2016-12-07 | 重庆市渝东南农业科学院 | A kind of selection of wide handle mustard cytoplasm male sterility line |
| CN106171948B (en) * | 2016-07-06 | 2019-01-04 | 重庆市渝东南农业科学院 | A kind of selection of width handle mustard cytoplasm male sterility line |
| CN109554497A (en) * | 2018-12-20 | 2019-04-02 | 中国农业科学院蔬菜花卉研究所 | PCR primer, kit and its application for the screening of wild cabbage Recessive male sterile gene |
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