CN102960242A - Breeding method for inducing parthenogenesis of corn by propyzamide - Google Patents
Breeding method for inducing parthenogenesis of corn by propyzamide Download PDFInfo
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- CN102960242A CN102960242A CN2012105454957A CN201210545495A CN102960242A CN 102960242 A CN102960242 A CN 102960242A CN 2012105454957 A CN2012105454957 A CN 2012105454957A CN 201210545495 A CN201210545495 A CN 201210545495A CN 102960242 A CN102960242 A CN 102960242A
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Abstract
The invention relates to a breeding method for inducing parthenogenesis of corn by propyzamide, belonging to the field of polyploid breeding of plants. The breeding method specifically comprises the following steps of: preparing an inducer with the content of 5 mu mol/L-200mu mol/L by the propyzamide and water; performing induction treatment on filaments, wherein the inducer is sprayed or smeared onto the filaments which grow to about 2.5cm long after emerging, and mature seeds are harvested to get genotype homozygous diploid seeds; and selecting and identifying the seeds according to a conventional method, and selecting a head progeny row which conforms to a breeding goal, thus finally getting a homozygous corn inbred line. The breeding method disclosed by the invention has the positive effects of omitting artificial pollination, being simple, being easy to implement, low in cost, high in efficiency and suitable for large-scale use.
Description
Technical field
The invention belongs to field of plant variety breeding technology, particularly the breeding method of corn inbred line.
Background technology
In the conventional method of corn inbred lines, the Breeding Process that finish an inbred line needs more than 7 generations of selfing at least, and is then more for the required generation of the material of genetic background complexity.In order to shorten the seed selection cycle, domestic and international existing way is that to adopt Stock 6 to induce be to carry out genetic induction, induce the Haploid production seed after, with chromosome doubling inorganic agents such as colchicines it is doubled into dliploid again; The chemical reagent processing filigrees such as the colchicine of employing are also arranged, and inducing maize directly produces the dliploid seed that isozygotys by parthenogenesis.But there is following shortcoming in these methods:
1.Stock need after 6 genetic induction methods are induced every seed is distinguished, find out the seed of inducing generation.Workload is large and uninteresting, and is difficult to realize mechanization.
2.Stock 6 pairs some induce and also have the not good problem of color developing effect, so almost illegible;
3.Stock 6 genetic induction methods induce the seed overwhelming majority of formation to belong to monoploid, needing just can become normal plant through chromosome doubling again, and the chromosome doubling technology is loaded down with trivial details, to add multiplying power lower;
4.Stock 6 genetic induction methods need artificial pollination, the links such as father, maternal intermission seeding, florescence adjusting.
5. use the colchicine-induced parthenogenesis, the carcinogenicity of reagent is strong, reagent cost is high, and health and Environmental security are threatened greatly, and necessary strict operation and control use are difficult to large tracts of land use in breeding.
The objective of the invention is:, lure corn into without double fertilization but directly form the dliploid seed by parthenogenesis as chemical inducer with propyzamide, and then obtain pure lines fast.Accelerate inbred line breeding speed, shortening the breeding cycle, reagent to the person poultry safety, easy and simple to handle, and greatly reduce the breeding cost.
Propyzamide, another name alkynes the third pethoxamid, pronamide, alkynes Stam F-34, pentyne grass amine, gram grass peace.English common name: Propyzamide.Chinese chemical name: N-(1,1-dimethyl propargyl)-3,5-two chloro-benzamides.Molecular formula: C12H11Cl2NO, molecular weight 256.1.Outward appearance is the colourless crystallization powder, solvability 15mg/L in 25 ℃ of lower water.Propyzamide 50% wetting powder outward appearance is white powder, is low toxicity herbicide.Kill monocotyledon weed by the mitosis of disturbing the weeds cell.
The propyzamide structural formula
Summary of the invention
The technical problem to be solved in the present invention is: utilize the inhibition cell division effect of propyzamide, inducing maize is without double fertilization but directly form seed by the parthenogenesis mode; Solve derivants such as in the past using colchicine to human body carcinogenic, cause a disease, cost is high, not easy to operate, and that the colour developing of the seed in the Stock 6 genetic induction methods is unstable, seed is differentiated is difficult, doubling monoploids is complicated and add the problems such as multiplying power is low.
The technical solution used in the present invention is as follows:
1. derivant preparation: the propyzamide aqueous solution of preparing 5 μ mol/L～200 μ mol/L;
2. induce processing: contain the phase at corn silking, cut short filigree, filigree length keeps 1～3 centimetre length, sprays or smears the propyzamide derivant, every day about 1～3 time, continuous about 1～4 day to filigree afterwards;
3. results, discriminating and breeding: gather in the crops during seed maturity, namely obtain the dliploid seed that genotype is isozygotied; Next season sowing, the bagging selfing is carried out authenticity according to phenotype, eliminates plant of poor quality, obtains single fringe; The fruit ear of results is continued the sowing next generation, form head progeny row, differentiate the head progeny row that isozygotys according to the regularity of the proterties such as plant, fruit ear, seed, select to meet the head progeny row results of breeding objective, i.e. the corn inbred line that acquisition is isozygotied.
Good effect of the present invention is as follows:
1. propyzamide toxicity is low, can use in the field under opening status, to people and animals and Environmental security;
2. inducing maize produces gynecogenic probability and is higher than existing other chemical inducers, and general inductivity can reach about 2.0%, and some examination material can be higher;
3. needn't pollinate, not exist contemporary seed to differentiate difficult problem;
Simple, the processing ease of method, cost is low, efficient is high, can use on a large scale;
5. directly form the dliploid seed, do not need the chromosome doubling process, program is simple;
6. more than shortening for 5 generations than the ordinary maize inbred line breeding method seed selection cycle.
7. method effect good reproducibility of the present invention, repeatability reaches more than 90% in the corn variety of routine.
Embodiment
Embodiment 1
(1) derivant preparation and processing material are selected
Select the 50% propyzamide wetting powder of selling on the market, be diluted with water to the derivant that concentration is 40 μ mol/L.
The processing object is corn hybrid seed " the Liao Dynasty single 565 ".
(2) induce processing
Before weaving silk to the strict bagging of female fringe.After most of filigree spues on the fruit ear, cut short filigree, filigree length keeps about 2.5 centimetres, sprays to filigree with miniaturised nebuliser afterwards, recovers paper bag.Repeat spray 2 time during respectively at 6:00 and 8:00 every day, processed continuously 2 days.Process 30 fringes.
(3) results
The results fruit ear is carefully peeled bract off when ripe, takes off seed.
(4) identify and breeding
Next season with the seed monoseeding, eliminate the individual plant do not meet breeding goal, the bagging selfing obtains 100 fruit ears; The fruit ear of results is continued the sowing next generation, plant into 100 head progeny rows, every head progeny row 50 strains, bagging selfing.According to the regularity of the proterties such as plant, fruit ear, seed and proterties whether meet breeding goal and select in excellent excellent, 70 head progeny rows of selecting and remain, i.e. 70 parts of inbred line materials that isozygoty.So far finish the Breeding Process of inbred line.
Embodiment 2
(1) derivant preparation and processing material are selected
Select the propyzamide of selling on the market, be diluted with water to the derivant that concentration is 60 μ mol/L.
The processing object is corn hybrid seed " Zheng Dan 958 ".
(2) induce processing
Before weaving silk to the strict bagging of female fringe.After most of filigree spues on the fruit ear, cut short filigree, filigree length keeps about 2.0 centimetres, sprays to filigree with miniaturised nebuliser afterwards, recovers at last paper bag.Repeat to spray 3 times every day respectively when 6:00,8:00 and 17:00, processed continuously 2 days.Process 40 fringes.
(3) results
The results fruit ear is carefully peeled bract off after ripe, takes off seed, obtains 180 in seed.
(4) identify and breeding
Next season with the seed monoseeding, select the good individual plant of phenotype, the bagging selfing obtains 150 fruit ears, passes through species test 130 fruit ears of selecting and remain.
The fruit ear of results is continued the sowing next generation, plant into 130 head progeny rows, every head progeny row 50 strains, bagging selfing.Whether purity and proterties according to proterties such as plant regularity and fruit ear, seeds meet breeding goal, select in excellent excellent, the bagging selfing, 80 head progeny rows selecting and remain are the inbred line material that isozygotys.So far finish the Breeding Process of inbred line.
Embodiment 3
(1) derivant preparation and processing material are selected
Select the propyzamide of selling on the market, be diluted with water to the derivant that concentration is 80 μ mol/L.
Process object and be " dredging single 20 ".
(2) induce processing
Sow 1000 meters space isolation conditions and to plant, the strict castration before the loose powder.After most of filigree spued on the fruit ear, filigree length was sprayed to the filigree position with sprayer when 2.0 centimetres of left and right sides.Repeat to spray 3 times at 6:00,8:00 and 16:00, processed continuously 2 days.Process 50 fringes.
(3) results
The results fruit ear is carefully peeled bract off after ripe, takes off seed, 200 in results seed.
(4) identify and breeding
Next season with the seed monoseeding, according to 150 strains of Phenotypic Selection fine individual plant, bagging selfing, 100 fruit ears of selecting and remain; It is of future generation to continue sowing, plants into 100 head progeny rows, every head progeny row 50 strains, bagging selfing.According to the purity of the proterties such as plant, fruit ear, seed and whether meet breeding goal, select in excellent excellently, 80 head progeny rows of selecting and remain namely obtain 80 parts of corn inbred lines that isozygoty.So far finish the Breeding Process of corn inbred line.
Embodiment 4
(1) derivant preparation and processing material are selected
Select the propyzamide of selling on the market, be diluted with water to the derivant that concentration is 120 μ mol/L.
Processing object is " middle list 909 ".
(2) induce processing
Strict bagging before weaving silk.After most of filigree spues on the fruit ear, cut short filigree, filigree length keeps 2～3 centimetres, sprays to the filigree position with sprayer afterwards.When 6:00 and 16:00, repeat 2 times, processed continuously 3 days.Process 50 fringes.
(3) results
The results fruit ear is carefully peeled bract off after ripe, takes off seed.200 in results seed.
(4) identify and breeding
Next season with the seed monoseeding, the bagging selfing.Eliminate bad individual plant and fruit ear according to phenotype, select in excellent excellent, 150 fruit ears of selecting and remain; The fruit ear of results is continued the sowing next generation, plant into 150 head progeny rows, every head progeny row 40 strains, bagging selfing.Whether purity and proterties according to proterties such as plant regularity and fruit ear, seeds meet breeding goal, and 80 head progeny rows of selecting and remain obtain the inbred line of isozygotying.So far finish the Breeding Process of corn inbred line.
Embodiment 5
(1) derivant preparation and processing material are selected
Select the propyzamide of selling on the market, be diluted with water to the derivant that concentration is 200 μ mol/L.
Processing object is " red beautiful 39 ".
(2) induce processing
Strict bagging before weaving silk.After most of filigree spues on the fruit ear, cut short filigree, filigree length keeps 2～3 centimetres, smears reagent with rayon balls to the filigree position afterwards.When 6:00 and 10:00, repeat 2 times, processed continuously 2 days.Process 50 fringes.
(3) results
The results fruit ear is carefully peeled bract off after ripe, takes off seed.140 in results seed.
(4) identify and breeding
Next season with the seed monoseeding, the bagging selfing.Eliminate bad individual plant and fruit ear according to phenotype, select in excellent excellent, bagging selfing, 100 fruit ears of selecting and remain after the results; The fruit ear of results is continued the sowing next generation, plant into 100 head progeny rows, every head progeny row 40 strains, bagging selfing.Whether purity and proterties according to proterties such as plant regularity and fruit ear, seeds meet breeding goal, and 70 head progeny rows of selecting and remain obtain the inbred line of isozygotying.So far finish the Breeding Process of corn inbred line.
Described " the Liao Dynasty single 565 ", " Zheng Dan 958 ", " dredging single 20 ", " middle list 909 " or " red beautiful 39 " all can be bought by the commercial channel of routine and obtain; because the method has higher repeatability, therefore the protection domain of invention is not limited to this above-mentioned kind.
Claims (2)
1. one kind with the gynecogenic breeding method of propyzamide inducing maize, it is characterized in that carrying out successively according to the following steps:
(1) derivant preparation: be mixed with the derivant that concentration is 5 μ mol/L～200 μ mol/L with propyzamide and water;
(2) induce processing: after filigree spued on the fruit ear, filigree length kept about 1.5 centimetres, then sprayed or smeared derivant, every day about 1～3 time, continuous about 1～4 day to filigree;
(3) results, evaluation and breeding: gather in the crops during seed maturity, after this carry out according to a conventional method seed selection, namely obtain the dliploid inbred line that genotype is isozygotied.
2. as claimed in claim 1 with the gynecogenic breeding method of propyzamide inducing maize, it is characterized in that: described corn variety is " the Liao Dynasty single 565 ", " Zheng Dan 958 ", " dredging single 20 ", " middle list 909 " or " red beautiful 39 ".
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886147A (en) * | 2015-06-04 | 2015-09-09 | 杨宪民 | Broad-spectrum corn parthenogernesis inducer |
| CN105359967A (en) * | 2015-11-26 | 2016-03-02 | 沈阳农业大学 | Breeding method for solving color difference problem of colored fresh-eating corn grains |
| CN105941140A (en) * | 2016-05-22 | 2016-09-21 | 沈阳农业大学 | Chemical induction corn parthenogenesis operation method |
| CN112772411A (en) * | 2021-02-24 | 2021-05-11 | 内蒙古民族大学 | Breeding method for inducing maize apomixis by using mixed reagent and application |
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| CN101377481A (en) * | 2008-10-06 | 2009-03-04 | 中国农业大学 | Method for identifying corn parthenogenesis haploidy |
| CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
| CN102550399A (en) * | 2011-12-22 | 2012-07-11 | 沈阳农业大学 | Breeding method for inducing parthenogenesis of corn by trifluralin |
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2012
- 2012-12-17 CN CN2012105454957A patent/CN102960242A/en active Pending
Patent Citations (4)
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| WO2001032001A1 (en) * | 1999-10-29 | 2001-05-10 | Utah State University | Methods for stabilizing and controlling apomixis |
| CN101377481A (en) * | 2008-10-06 | 2009-03-04 | 中国农业大学 | Method for identifying corn parthenogenesis haploidy |
| CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886147A (en) * | 2015-06-04 | 2015-09-09 | 杨宪民 | Broad-spectrum corn parthenogernesis inducer |
| CN104886147B (en) * | 2015-06-04 | 2017-11-14 | 杨宪民 | Broad spectrum activity corn parthenogenesis-inducing regulator |
| CN105359967A (en) * | 2015-11-26 | 2016-03-02 | 沈阳农业大学 | Breeding method for solving color difference problem of colored fresh-eating corn grains |
| CN105941140A (en) * | 2016-05-22 | 2016-09-21 | 沈阳农业大学 | Chemical induction corn parthenogenesis operation method |
| CN112772411A (en) * | 2021-02-24 | 2021-05-11 | 内蒙古民族大学 | Breeding method for inducing maize apomixis by using mixed reagent and application |
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Application publication date: 20130313 |