CN103081804A - Breeding method for introducing corn parthenogernesis by using amiprofos-methyl - Google Patents
Breeding method for introducing corn parthenogernesis by using amiprofos-methyl Download PDFInfo
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- CN103081804A CN103081804A CN2012105455288A CN201210545528A CN103081804A CN 103081804 A CN103081804 A CN 103081804A CN 2012105455288 A CN2012105455288 A CN 2012105455288A CN 201210545528 A CN201210545528 A CN 201210545528A CN 103081804 A CN103081804 A CN 103081804A
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Abstract
The invention relates to a breeding method for introducing corn parthenogernesis by using amiprofos-methyl, and belongs to the field of planting breeding. The method comprises the following steps of: preparing an inductive agent of which the concentration is 5-200mumol/L from the amiprofos-methyl and water; performing introducing treatment: when filaments sprout to grow to about 2.5mm, spraying or daubing the inductive agent to the filaments, and harvesting to obtain homozygous genotype diploid seeds when seeds are mature; and during seeding in the next season, selecting and identifying by using the conventional method to select head progeny rows according with the breeding aim to obtain a homozygous corn selfing line. The breeding method for introducing the corn parthenogernesis by using the amiprofos-methyl has the positive effects of avoiding the artificial pollination, and being simple in method, easy to operate, low in cost, high in efficiency, and capable of being applied in large scale; in addition, the diploid seeds are directly formed and a chromosome doubling process is not required; and in the breeding method, more than 5 generations are shortened in comparison with a breeding method of the conventional corn selfing line.
Description
Technical field
The invention belongs to field of plant variety breeding technology, particularly the breeding method of corn inbred line.
Background technology
In the conventional method of corn inbred lines, more than the Breeding Process that complete an inbred line needs 7 generations of selfing at least, more for the required generation of the material of genetic background complexity.In order to shorten the seed selection cycle, domestic and international existing way is to adopt Stock6 to induce to be to carry out genetic induction, after inducing the Haploid production seed, then with chromosome doubling inorganic agents such as colchicines, it to be doubled into dliploid; The chemical reagent processing filigrees such as the colchicine of employing are also arranged, and inducing maize directly produces by parthenogenesis the dliploid seed that isozygotys.But there is following shortcoming in these methods:
1.Stock6 need after the genetic induction method is induced every seed is distinguished, find out by inducing the seed of generation.Workload is large and uninteresting, and is difficult to realize mechanization.
2.Stock6 some is induced also have the not good problem of color developing effect, so almost illegible;
3.Stock6 the genetic induction method induces the seed overwhelming majority of formation to belong to monoploid, needing just can become normal plant through chromosome doubling again, and the chromosome doubling technology is loaded down with trivial details, to add multiplying power lower;
4.Stock6 the genetic induction method needs artificial pollination, the links such as father, maternal intermission seeding, florescence adjusting.
5. use the colchicine-induced parthenogenesis, the carcinogenicity of reagent is strong, reagent cost is high, and is large to health and Environmental security threat, and necessary strict operation and control are used, and is difficult to large tracts of land use in breeding.
The objective of the invention is:, lure corn into without double fertilization but directly form the dliploid seed by parthenogenesis as chemical inducer with amiprophos-methyl, and then obtain pure lines fast.Accelerate inbred line breeding speed, shortening the breeding cycle, reagent to the person poultry safety, easy and simple to handle, and greatly reduce the breeding cost.
Amiprophos-methyl, English name: amiprophos-methyl.Molecular formula: C11H17N2O4PS, molecular weight 304.3.White crystals, 64～65 ℃ of fusing points.Solvability in water is 10mg/L, and preparation is generally 60% wetting powder.Be used for the field chemical weed control.
Summary of the invention
The technical problem to be solved in the present invention is: utilize the inhibition cell division effect of amiprophos-methyl, inducing maize is without double fertilization but directly form seed by the parthenogenesis mode; Solve derivants such as in the past using colchicine to human body carcinogenic, cause a disease, cost is high, not easy to operate, and that the colour developing of the seed in Stock6 genetic induction method is unstable, seed is differentiated is difficult, doubling monoploids is complicated and add the problems such as multiplying power is low.
The technical solution used in the present invention is as follows:
1. derivant preparation: the amiprophos-methyl aqueous solution of preparation 5 μ mol/L～200 μ mol/L;
2. induce processing: contain the phase at corn silking, cut short filigree, filigree length keeps 1～3 centimetre length, spray or smear the amiprophos-methyl derivant to filigree afterwards, every day 1～3 left and right, continuous about 1～4 day;
3. results, discriminating and breeding: gather in the crops during seed maturity, namely obtain the dliploid seed that genotype is isozygotied; Next season sowing, the bagging selfing is carried out authenticity according to phenotype, eliminates plant of poor quality, obtains single fringe; The fruit ear of results is continued the sowing next generation, form head progeny row, differentiate according to the regularity of the proterties such as plant, fruit ear, seed the head progeny row that isozygotys, select to meet the head progeny row results of breeding objective, i.e. the corn inbred line that acquisition is isozygotied.
Good effect of the present invention is as follows:
1. amiprophos-methyl toxicity is low, can use in the field under opening status, to people and animals and Environmental security;
2. inducing maize produces gynecogenic probability higher than existing other chemical inducers, and general inductivity can reach 2.0% left and right, and some examination material can be higher;
3. needn't pollinate, not exist contemporary seed to differentiate difficult problem;
Simple, the processing ease of method, cost is low, efficient is high, can use on a large scale;
5. directly form the dliploid seed, do not need the chromosome doubling process, program is simple;
6. more than shortening for 5 generations than the ordinary maize inbred line breeding method seed selection cycle.
7. the method has higher repeatability, all realizes the product sexual reproduction in the corn variety of routine.
Embodiment
Embodiment 1
(1) derivant preparation and processing material are selected
Select the amiprophos-methyl of selling on market, be diluted with water to the derivant that concentration is 40 μ mol/L.
The processing object is corn hybrid seed " the Liao Dynasty single 565 ".
(2) induce processing
Before weaving silk to the strict bagging of female fringe.After on fruit ear, most of filigree spues, cut short filigree, filigree length keeps 2.5 centimetres of left and right, sprays to filigree with miniaturised nebuliser afterwards, recovers paper bag.Repeat spray 2 time respectively at 6:00 and 8:00 every day, processed continuously 2 days.Process 30 fringes.
(3) results
When ripe, the results fruit ear, carefully peel bract off, takes off seed.
(4) identify and breeding
With the seed monoseeding, eliminate the individual plant that does not meet breeding goal in next season, the bagging selfing obtains 100 fruit ears; The fruit ear of results is continued the sowing next generation, plant into 100 head progeny rows, every head progeny row 50 strains, bagging selfing.According to the regularity of the proterties such as plant, fruit ear, seed and proterties whether meet breeding goal and select in excellent excellent, 70 head progeny rows of selecting and remain, i.e. 70 parts of inbreds that isozygoty.So far complete the Breeding Process of inbred line.
Embodiment 2
(1) derivant preparation and processing material are selected
Select the amiprophos-methyl of selling on market, be diluted with water to the derivant that concentration is 60 μ mol/L.
The processing object is corn hybrid seed " Zheng Dan 958 ".
(2) induce processing
Before weaving silk to the strict bagging of female fringe.After on fruit ear, most of filigree spues, cut short filigree, filigree length keeps 2.0 centimetres of left and right, sprays to filigree with miniaturised nebuliser afterwards, recovers at last paper bag.Repeat to spray 3 times at 6:00,8:00 and 17:00 every day respectively, processed continuously 2 days.Process 40 fringes.
(3) results
After ripe, the results fruit ear, carefully peel bract off, takes off seed, obtains 180, seed.
(4) identify and breeding
With the seed monoseeding, select the good individual plant of phenotype in next season, the bagging selfing obtains 150 fruit ears, passes through species test 130 fruit ears of selecting and remain.
The fruit ear of results is continued the sowing next generation, plant into 130 head progeny rows, every head progeny row 50 strains, bagging selfing.Whether meet breeding goal according to purity and the proterties of the proterties such as plant regularity and fruit ear, seed, select in excellent excellent, the bagging selfing, 80 head progeny rows selecting and remain are the inbred that isozygotys.So far complete the Breeding Process of inbred line.
Embodiment 3
(1) derivant preparation and processing material are selected
Select the amiprophos-methyl of selling on market, be diluted with water to and contain the derivant that concentration is 80 μ mol.
Process object and be " dredging single 20 ".
(2) induce processing
Sow 1000 meters space isolation conditions and plant, strict emasculation before loose powder.After on fruit ear, most of filigree spued, filigree length used sprayer to filigree position spraying when 2.0 centimetres of left and right.Repeat to spray 3 times at 6:00,8:00 and 16:00, processed continuously 2 days.Process 50 fringes.
(3) results
After ripe, the results fruit ear, carefully peel bract off, takes off seed, 200, results seed.
(4) identify and breeding
In next season with the seed monoseeding, according to 150 strains of Phenotypic Selection fine individual plant, bagging selfing, 100 fruit ears of selecting and remain; Continue sowing of future generation, plant into 100 head progeny rows, every head progeny row 50 strains, bagging selfing.According to the purity of the proterties such as plant, fruit ear, seed and whether meet breeding goal, select in excellent excellently, 80 head progeny rows of selecting and remain namely obtain 80 parts of corn inbred lines that isozygoty.So far complete the Breeding Process of corn inbred line.
Embodiment 4
(1) derivant preparation and processing material are selected
Select the amiprophos-methyl of selling on market, be diluted with water to the derivant that concentration is 120 μ mol/L.
Processing object is " middle list 909 ".
(2) induce processing
Strict bagging before weaving silk.After on fruit ear, most of filigree spues, cut short filigree, filigree length keeps 2～3 centimetres, uses afterwards sprayer to filigree position spraying.Repeat 2 times at 6:00 and 16:00, processed continuously 3 days.Process 50 fringes.
(3) results
After ripe, the results fruit ear, carefully peel bract off, takes off seed.200, results seed.
(4) identify and breeding
In next season with the seed monoseeding, the bagging selfing.Eliminate bad individual plant and fruit ear according to phenotype, select in excellent excellent, 150 fruit ears of selecting and remain; The fruit ear of results is continued the sowing next generation, plant into 150 head progeny rows, every head progeny row 40 strains, bagging selfing.Whether meet breeding goal, 80 head progeny rows of selecting and remain, the corn inbred line that obtains isozygotying according to purity and the proterties of the proterties such as plant regularity and fruit ear, seed.So far complete the Breeding Process of corn inbred line.
Embodiment 5
(1) derivant preparation and processing material are selected
Select the amiprophos-methyl of selling on market, be diluted with water to the derivant that concentration is 200 μ mol/L.
Processing object is " red beautiful 39 ".
(2) induce processing
Strict bagging before weaving silk.After on fruit ear, most of filigree spues, cut short filigree, filigree length keeps 2～3 centimetres, smears reagent with rayon balls to the filigree position afterwards.Repeat 2 times at 6:00 and 10:00, processed continuously 2 days.Process 50 fringes.
(3) results
After ripe, the results fruit ear, carefully peel bract off, takes off seed.140, results seed.
(4) identify and breeding
In next season with the seed monoseeding, the bagging selfing.Eliminate bad individual plant and fruit ear according to phenotype, select in excellent excellent, bagging selfing, 100 fruit ears of selecting and remain after results; The fruit ear of results is continued the sowing next generation, plant into 100 head progeny rows, every head progeny row 40 strains, bagging selfing.Whether meet breeding goal, 70 head progeny rows of selecting and remain, the corn inbred line that obtains isozygotying according to purity and the proterties of the proterties such as plant regularity and fruit ear, seed.So far complete the Breeding Process of corn inbred line.
Described " the Liao Dynasty single 565 ", " Zheng Dan 958 ", " dredging single 20 ", " middle list 909 " or " red beautiful 39 " all can be bought by the commercial channel of routine and obtain; because the method has higher repeatability, therefore the protection domain of invention is not limited to this above-mentioned kind.
Claims (2)
1. one kind with the gynecogenic breeding method of amiprophos-methyl inducing maize, it is characterized in that carrying out successively according to the following steps:
A. derivant preparation: the amiprophos-methyl aqueous solution of preparation 5 μ mol/L～200 μ mol/L;
B. induce processing: contain the phase at corn silking, cut short filigree, filigree length keeps 1～3 centimetre length, spray or smear the amiprophos-methyl derivant to filigree afterwards, every day 1～3 left and right, continuous about 1～4 day;
C. gather in the crops, differentiate and breed: gather in the crops during seed maturity, namely obtain the dliploid seed that genotype is isozygotied; Next season sowing, the bagging selfing is carried out authenticity according to phenotype, eliminates plant of poor quality, obtains single fringe; The fruit ear of results is continued the sowing next generation, form head progeny row, differentiate according to the regularity of the proterties such as plant, fruit ear, seed the head progeny row that isozygotys, select to meet the head progeny row results of breeding objective, i.e. the corn inbred line that acquisition is isozygotied.
2. as claimed in claim 1 with the gynecogenic breeding method of amiprophos-methyl inducing maize, it is characterized in that: described corn variety is " the Liao Dynasty single 565 ", " Zheng Dan 958 ", " dredging single 20 ", " middle list 909 " or " red beautiful 39 ".
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103733979A (en) * | 2013-12-23 | 2014-04-23 | 中国科学院东北地理与农业生态研究所 | Application of DNA methylation inhibitor in improving induction efficiency of corn male parent haploid induction system |
| CN103999769A (en) * | 2014-06-06 | 2014-08-27 | 山东省农业科学院玉米研究所 | Corn haploid inducing method |
| CN104604673A (en) * | 2014-08-18 | 2015-05-13 | 沈阳特亦佳玉米科技有限公司 | Treatment method for inducing maize parthenogenesis with colchicine |
| CN104604674A (en) * | 2014-08-18 | 2015-05-13 | 沈阳特亦佳玉米科技有限公司 | Treating method for chemically inducing parthenogenesis of corn |
| CN105359967A (en) * | 2015-11-26 | 2016-03-02 | 沈阳农业大学 | Breeding method for solving color difference problem of colored fresh-eating corn grains |
| CN107246987A (en) * | 2017-06-06 | 2017-10-13 | 南京农业大学 | A kind of method of Helminthosporium sativum chromosome sectioning |
| CN107593436A (en) * | 2017-10-31 | 2018-01-19 | 山西省农业科学院玉米研究所 | Utilize the selection of pendimethalin inducing maize single-female generation |
| CN112841027A (en) * | 2021-03-08 | 2021-05-28 | 内蒙古民族大学 | Breeding method for inducing maize apomixis by using polyethyleneimine and application |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1245636A (en) * | 1998-08-24 | 2000-03-01 | 中国科学院遗传研究所 | Method for producing corn hybrid seed by utilizing distant hybridization and parthenogenetic reproduction |
| WO2001032001A1 (en) * | 1999-10-29 | 2001-05-10 | Utah State University | Methods for stabilizing and controlling apomixis |
| CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
| CN102550399A (en) * | 2011-12-22 | 2012-07-11 | 沈阳农业大学 | Breeding method for inducing parthenogenesis of corn by trifluralin |
-
2012
- 2012-12-17 CN CN2012105455288A patent/CN103081804A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1245636A (en) * | 1998-08-24 | 2000-03-01 | 中国科学院遗传研究所 | Method for producing corn hybrid seed by utilizing distant hybridization and parthenogenetic reproduction |
| WO2001032001A1 (en) * | 1999-10-29 | 2001-05-10 | Utah State University | Methods for stabilizing and controlling apomixis |
| CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
| CN102550399A (en) * | 2011-12-22 | 2012-07-11 | 沈阳农业大学 | Breeding method for inducing parthenogenesis of corn by trifluralin |
Non-Patent Citations (1)
| Title |
|---|
| 惠国强等: ""不同除草剂加倍玉米单倍体的效率"", 《作物学报》 * |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103733979A (en) * | 2013-12-23 | 2014-04-23 | 中国科学院东北地理与农业生态研究所 | Application of DNA methylation inhibitor in improving induction efficiency of corn male parent haploid induction system |
| CN103733979B (en) * | 2013-12-23 | 2015-06-03 | 中国科学院东北地理与农业生态研究所 | Method for improving induction efficiency of corn male parent haploid induction system |
| CN103999769A (en) * | 2014-06-06 | 2014-08-27 | 山东省农业科学院玉米研究所 | Corn haploid inducing method |
| CN103999769B (en) * | 2014-06-06 | 2016-06-01 | 山东省农业科学院玉米研究所 | A kind of corn haploid induction method |
| CN104604673A (en) * | 2014-08-18 | 2015-05-13 | 沈阳特亦佳玉米科技有限公司 | Treatment method for inducing maize parthenogenesis with colchicine |
| CN104604674A (en) * | 2014-08-18 | 2015-05-13 | 沈阳特亦佳玉米科技有限公司 | Treating method for chemically inducing parthenogenesis of corn |
| CN104604674B (en) * | 2014-08-18 | 2017-05-10 | 沈阳特亦佳玉米科技有限公司 | Treating method for chemically inducing parthenogenesis of corn |
| CN105359967A (en) * | 2015-11-26 | 2016-03-02 | 沈阳农业大学 | Breeding method for solving color difference problem of colored fresh-eating corn grains |
| CN107246987A (en) * | 2017-06-06 | 2017-10-13 | 南京农业大学 | A kind of method of Helminthosporium sativum chromosome sectioning |
| CN107593436A (en) * | 2017-10-31 | 2018-01-19 | 山西省农业科学院玉米研究所 | Utilize the selection of pendimethalin inducing maize single-female generation |
| CN112841027A (en) * | 2021-03-08 | 2021-05-28 | 内蒙古民族大学 | Breeding method for inducing maize apomixis by using polyethyleneimine and application |
| CN112841027B (en) * | 2021-03-08 | 2022-06-28 | 内蒙古民族大学 | Breeding method for inducing maize apomixis by using polyethyleneimine and application |
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Application publication date: 20130508 |