CN107593436A - Utilize the selection of pendimethalin inducing maize single-female generation - Google Patents
Utilize the selection of pendimethalin inducing maize single-female generation Download PDFInfo
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- CN107593436A CN107593436A CN201711046859.6A CN201711046859A CN107593436A CN 107593436 A CN107593436 A CN 107593436A CN 201711046859 A CN201711046859 A CN 201711046859A CN 107593436 A CN107593436 A CN 107593436A
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- pendimethalin
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- filigree
- selection
- inducing maize
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Abstract
The invention discloses a kind of selection using pendimethalin inducing maize single-female generation, belong to corn breeding techniques field.Utilize the selection of pendimethalin inducing maize single-female generation：After corn emasculation after female fringe filigree discharge, cut in bract top end with dop clamp so that internal filigree is neatly broken, and extracts fracture filigree out, is formed segment cavity on bract top, is injected pendimethalin derivant, harvest dries in time after seed maturity.Derivant toxicity of the present invention is lower, and inductivity is higher, sees that light is not easily decomposed, and can guarantee that the stability of its germ plasm resource hereditary quality.
Description
Technical field
The invention belongs to corn breeding techniques field, is related to a kind of seed selection using pendimethalin inducing maize single-female generation
Method.
Background technology
Corn is China the first generalized grain crop, has that unit area biological yield and grain yield are high, yield potential and
Genetic improvement pot ential is huge, wide adaptability, nutritious, the features such as being widely used, in national economy, national food security guarantee
In play an important roll.
The raising of corn total output is mainly attributed to the popularization and application of cenospecies, i.e., miscellaneous by the strong advantage of genetic improvement seed selection
Hand over kind, improve constantly the yield potential and sown area of corn variety, realize the constantly improve of per unit area yield and total yield.However, corn
Breeding is one periodically long, and complexity is strong, the big work of difficulty.Traditional selection is by inbred line breeding, assembled
Combine and tentatively identified, try to authorize by area, then marketing, at least need the 8-10 years, wherein self-mating system is selected
Educate be most time and effort consuming in breeding process work.
In order to improve breeding efficiency, accelerate the seed selection speed of excellent self-mating system, prior art uses the life with mark color
Thing induction is Stock-6 to carry out haploid induction, though domestic also expand using chemical drugs such as trefanocide, colchicines at present
Agent smearing filigree, which is induced, directly produces homozygotic research, but above method has the shortcomings that certain, and its shortcoming is as follows：
（1）The offspring induced using Stock-6 biologies revulsion the corn of some special colors, it marks color to be difficult to,
Such as the germplasm materials of the special color such as black, purple, red；（2）Mean using Stock-6 biology inductions foreign heredity substance
Target germ plasm resource is imported into, the quality of its germ plasm resource can be influenceed to a certain extent, especially in some Chemical mutagenesis,
Such as eat sweet-waxy maizes raw, pop corn, silage corn etc.；（3）Using Stock-6 biology induction time and effort consumings, cost is higher,
Efficiency is low；（4）Colchicine effect in chemical induction is preferable, but because being poisonous reagent, with high costs and to people and animals, environment
Deng having larger potential safety hazard, it is difficult to the large-scale use during corn breeding；（5）Trefanocide is in the lonely female life of inducing maize
Growing in initiative germ plasm resource has certain dissemination, but because its is volatile, sees the light easily drawback such as decomposition also to a certain degree
Upper influence inducing effect.
It is inducing maize breeding material selecting and breeding corn self-mating system using corn single-female generation induction in corn breeding field,
This technology is also referred to as haploid breeding technology, can extremely be significantly reduced the seed selection of corn inbred line and corn variety
The time limit, significantly improve breeding efficiency.
The content of the invention
It is with diformazan it is an object of the invention to provide a kind of selection using pendimethalin inducing maize single-female generation
Penta spirit is used as derivant, and inducing maize directly produces diploid seed without zoogamy, so as to reach quickening breeding process
Purpose.
The present invention is achieved by the following technical solutions：
Utilize the selection of pendimethalin inducing maize single-female generation：After corn emasculation after female fringe filigree discharge, in bud
Leaf top end is cut with dop clamp so that internal filigree is neatly broken, and extracts fracture filigree out, and it is empty to form segment on bract top
Chamber, pendimethalin derivant is injected, harvest dries in time after seed maturity.
Further, described pendimethalin derivant be pendimethalin missible oil is watered the 100umol/L that is diluted to~
200umol/L dilutions, injection volume are 2~3mL.
Pendimethalin derivant is that pendimethalin micro-capsule suspension is watered to the 100umol/L~200umol/ being diluted to
L dilutions, injection volume are 2~3mL.
Described corn after female fringe filigree spues 3~5 days, with nipper plier, cut at 2~3cm of bract top, reaches by folder
Nipper plier and bract at bract contact interface be not damaged, the effect that internal filigree is neatly broken.
Binding clip length >=5cm of described nipper plier.
Pendimethalin of the present invention, also known as Pendimethalin, apply field mend, dish grass spirit, pendimethalin, chemical name N- (1- ethyls
Propyl group) -2,6- dinitro -3,4- dimethylanilines, molecular formula：C13H19N3O4, weeds cell mitogen can be suppressed, see light not
Easily decompose, can induce the method that corn formulates germ plasm resource without zoogamy, solve other chemical agents such as autumn waters -- limid eyes
Celestial element severe toxicity, the shortcomings that trefanocide easy photodissociation.
I.e. the present invention suppresses the principle of weeds cell mitogen using pendimethalin and its sees the characteristic that light is not easily decomposed,
Inducing maize directly produces diploid seed without zoogamy, the interference of no Exotic Germplasm Resources inhereditary material, and then soon
Speed enters in the process of the stable homozygous line of screening, accelerates breeding process, carries out extensive new varieties utilization.
Compared with prior art, the present invention has advantages below：
（1）Pendimethalin belongs to a kind of less toxic reagent in itself as conventional herbicide, and concentration is lower after being diluted to derivant, toxicity
Lower, to people and animals, birds, insects etc. are safety low-poison；
（2）Pendimethalin its inductivity in corn single-female generation induction is suitable with trefanocide, in some Special germplasm resource materials
Nearly 50% is up on material；
（3）Pendimethalin is shown in that light is not easily decomposed, and use can be preserved in relatively long time after configuring induction liquid, and trefanocide
Then need now with the current；
（4）It is time saving and energy saving to monoploid identification process is not present after the Germplasms induction of special color, can extensive field
Between operate application；
（5）The interference that Exotic Germplasm Resources inhereditary material is not present in offspring is induced, can guarantee that its germ plasm resource hereditary quality
Stability.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
Embodiment 1 utilizes the selection of pendimethalin inducing maize single-female generation
（1）Induction agent and the selection of corn germplasm material：From 330g/L on the market pendimethalin missible oil, it is watered dilute
It is interpreted into 120umol/L dilution；
（2）Handle material：From " sweet tea+glutinous " black sweet tea of fresh edible maize cenospecies glutinous 631；
（3）Induction is handled：Under the open condition of crop field, strict female fringe bagging and emasculation before spinning, after filigree spues 3~5 days, use
A length of more than the 5cm of binding clip nipper plier is cut at 2~3cm of bract top with appropriately sized power folder, reaches nipper plier and bract
Bract is not damaged at contact interface, and after the effect that internal filigree is neatly broken, it is small in the formation one of bract top to extract fracture filigree out
2~3ml of above-mentioned induction liquid injection cavitys are extracted with syringe and recover paper bag in time after section cavity body；The fringe of coprocessing 100；
（4）Harvest：Fruit ear is checked and harvested in time when seed maturity, peels bract off, is removed after being photographed to record to fruit ear
Seed, 120, seed is harvested altogether；
（5）Identification and breeding：Other variegated seeds of reject before being sowed in next season, by the black sweet tea with objective trait, glutinous kind
Progress tabling is selected after son carries out culturing and transplanting seedlings crop field, and each individual plant for meeting objective trait is selfed, obtains 75 fruits
Fringe, plant into 75 head progeny rows, per 15 plants of head progeny row, according to the neat and consistent of the characters such as breeding time, plant, fruit ear, seed and other
Whether character meets selected objective target character, selected 30 head progeny rows, i.e., 30 parts homozygous stable Germplasms, so far completes whole
The initiative process of individual germ plasm resource.
Embodiment 2 utilizes the selection of pendimethalin inducing maize single-female generation
（1）Induction agent and the selection of corn germplasm material：Suspended from 450g/L on the market pendimethalin microcapsules
Agent, it is watered the dilution for being diluted to 200umol/L；
（2）Handle material：From corn hybrid seed " first jade 335 "；
（3）Induction is handled：Under the open condition of crop field, strict female fringe bagging and emasculation before spinning, after filigree spues 3~5 days, use
A length of more than the 5cm of binding clip nipper plier is cut at 2~3cm of bract top with appropriately sized power folder, reaches nipper plier and bract
Bract is not damaged at contact interface, and after the effect that internal filigree is neatly broken, it is small in the formation one of bract top to extract fracture filigree out
2~3ml of above-mentioned induction liquid injection cavitys are extracted with syringe and recover paper bag in time after section cavity body；The fringe of coprocessing 50；
（4）Harvest：Fruit ear is checked and harvested in time when seed maturity, peels bract off, is removed after being photographed to record to fruit ear
Seed, 47, seed is harvested altogether；
（5）Identification and breeding：In next season by seed monoseeding, after eliminating bad individual plant according to phenotype selfing obtain 45 fruits
Fringe, 38 fruit ears are selected and remain by species test, the fruit ear of harvest is continued into the sowing next generation, plants into individual 38 head progeny row, per 17 plants of head progeny row, root
Whether meet breeding goal according to the purity and character of the character such as plant regularity and fruit ear, seed, the selfing based material of conjunction, extremely
This completes the Breeding Process of self-mating system.
Claims (5)
- A kind of 1. selection using pendimethalin inducing maize single-female generation, it is characterised in that：Female after corn emasculation After Honoka kibisso goes out, cut in bract top end with dop clamp so that internal filigree is neatly broken, and fracture filigree is extracted out, in bud Segment cavity is formed at leaf top, injects pendimethalin derivant, and harvest dries in time after seed maturity.
- 2. the selection according to claim 1 with pendimethalin inducing maize single-female generation, it is characterised in that described Pendimethalin derivant be that pendimethalin missible oil is watered to the 100umol/L~200umol/L dilutions being diluted to, injection volume For 2~3mL.
- 3. the selection according to claim 1 with pendimethalin inducing maize single-female generation, it is characterised in that diformazan Penta clever derivant is that pendimethalin micro-capsule suspension is watered to the 100umol/L~200umol/L dilutions being diluted to, injection Measure as 2~3mL.
- 4. the selection according to claim 1 or 2 with pendimethalin inducing maize single-female generation, it is characterised in that Described corn is after female fringe filigree spues 3~5 days, and with nipper plier, folder is cut at 2~3cm of bract top.
- 5. the selection according to claim 4 with pendimethalin inducing maize single-female generation, it is characterised in that described Nipper plier binding clip length >=5cm.
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| CN201711046859.6A CN107593436A (en) | 2017-10-31 | 2017-10-31 | Utilize the selection of pendimethalin inducing maize single-female generation |
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| CN201711046859.6A CN107593436A (en) | 2017-10-31 | 2017-10-31 | Utilize the selection of pendimethalin inducing maize single-female generation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772411A (en) * | 2021-02-24 | 2021-05-11 | 内蒙古民族大学 | Breeding method for inducing maize apomixis by using mixed reagent and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081804A (en) * | 2012-12-17 | 2013-05-08 | 沈阳农业大学 | Breeding method for introducing corn parthenogernesis by using amiprofos-methyl |
| CN105941140A (en) * | 2016-05-22 | 2016-09-21 | 沈阳农业大学 | Chemical induction corn parthenogenesis operation method |
-
2017
- 2017-10-31 CN CN201711046859.6A patent/CN107593436A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081804A (en) * | 2012-12-17 | 2013-05-08 | 沈阳农业大学 | Breeding method for introducing corn parthenogernesis by using amiprofos-methyl |
| CN105941140A (en) * | 2016-05-22 | 2016-09-21 | 沈阳农业大学 | Chemical induction corn parthenogenesis operation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112772411A (en) * | 2021-02-24 | 2021-05-11 | 内蒙古民族大学 | Breeding method for inducing maize apomixis by using mixed reagent and application |
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Application publication date: 20180119 |