CN110089420A - A kind of selection of corn haploid induction line - Google Patents
A kind of selection of corn haploid induction line Download PDFInfo
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- CN110089420A CN110089420A CN201910444056.9A CN201910444056A CN110089420A CN 110089420 A CN110089420 A CN 110089420A CN 201910444056 A CN201910444056 A CN 201910444056A CN 110089420 A CN110089420 A CN 110089420A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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Abstract
The invention discloses a kind of selections of corn haploid induction line; it is to induce to be come transformation using the parents of well developed root system, the commercial hybrids kind that stalk is sturdy, plant type is compact, resistance to close, anti-fall, resistant to high temperatures, pollen amount is big, the loose powder time is long; and the induction system of two kinds of good Different groups of transformation is made into cenospecies; monoploid breeding using this cenospecies as novel induction system, applied to scale.Induction that the present invention generates system have the advantages that well developed root system, stalk are sturdy, plant type is compact, it is resistance to it is close, anti-fall, resistant to high temperatures, pollen amount is big, the loose powder time is long, can effectively expand sizable application, reduction risk when carrying out monoploid engineering breeding.
Description
Technical field
The invention belongs to breeding technical fields, and in particular to a kind of selection of corn haploid induction line.
Background technique
Haploid Breeding of Maize is to be pollinated using corn haploid induction line to basic material, induces Haploid production seed
Grain, further according to mark property, monoploid seed is selected, and the chromosome doublings such as doubles by Natural double or artificial chemistry medicament
Means make haploid chromosomes restore normal chromosomal number and become a kind of breeding method of self-mating system (DH system).Haploid breeding
Technology is applied because of its fast and efficiently advantage by more and more breeders in practice.
The inductivity of not isogeneous induction system is different, and high reachable 15% or more, low only 1% or so.Majority induction is root
Be it is weak, stalk is soft, and plant type is poor, easily lodges when planting density is higher；Meanwhile Tassel-Branch is few, pollen amount is small, under hot environment
Pollen activity is poor.Using when mostly artificial pollination, carry out monoploid engineering breeding when be difficult to effective expansion scale.To understand
Certainly problem above, it is thus proposed that do induction system after being hybridized with two kinds of not isogeneous induction systems and use, system all derives from substantially due to induction
Stock6, most breeders are more the raisings for focusing on inductivity when improvement induction is, do not consider germ plasm resource used
Monoid, therefore, even if two different induction systems hybridization, advantage is also less obvious, and still there is biggish risk in when use.
Summary of the invention
In order to solve the problems, such as background technique, the object of the present invention is to provide a kind of corn haploid induction lines
Selection, it is intended to the induction system made have well developed root system, stalk is sturdy, plant type is compact, resistance to close, anti-fall, resistant to high temperatures, colored
The advantage that powder amount is big, the loose powder time is long.
To realize goal of the invention, the present invention adopts the following technical scheme:
A kind of selection of corn haploid induction line, it is characterized in that: select high-frequency induction system Feng Legao lure No. 3,
It selects the commercial hybrids kind promoted in production, self-mating system F049 is selected to be haploid-induction test system；Utilize commercial hybrids kind
Parents' transformation induce system, using hybridization, selfing, backcrossing means during transformation, and combine the survey of single plant inductivity in the process
Fixed, seed and plant color mark are selected；The induction of two kinds of good Different groups of transformation be A and induction to be that B is carried out again miscellaneous
It hands over, does induction system using Hybrid F1 generation.
The Feng Legao lure No. 3 be the autonomous breeding of Feng Le kind industry induction system, to stablize self-mating system, inductivity is 12% left
It is right.The commercial hybrids kind should have that well developed root system, stalk are sturdy, plant type is compact, resistance to close, anti-fall, resistant to high temperatures, pollen amount
Greatly, the advantage of loose powder time length；And the maternal A and male parent B of the commercial hybrids kind should not also have critical defect.The F049 is rich
The happy free self-mating system of kind of industry, it is particularly evident with the endosperm aleurone and embryo point purple label that hybridize seed after the pollination of induction system, and
Then only aleurone has purple to monoploid, and it is simple, accurate to select monoploid without purple for embryo point.
Specifically, the selection includes the following steps:
(1) No. 3 are lured to assemble F1 with Feng Legao respectively with the maternal A of commercial hybrids kind and male parent B, the building form of F1 is A
× Feng Legao lures No. 3 and B × Feng Legao to lure No. 3 or reciprocal cross；
(2) A × Feng Legao lure No. 3 and B × Feng Legao lure No. 3 it is each carry out 2 generations or inbreeding of more generation, using single while selfing
The test of times body inductivity is that F049 measurement A × Feng Legao lures No. 3 and B × Feng Legao inductivities for luring No. 3 separation offsprings, selects it
The middle highest single plant of inductivity continues plus generation, until there is inductivity up to 10% or more single plant；
(3) in the suitable generation, from the separation offspring that A × Feng Legao lures No. 3, the highest single plant of inductivity is selected to be mother
This, is returned by male parent of A, obtains (A × Feng Legao lures No. 3) F3BC1 or (A × Feng Legao lures No. 3) F4BC1；Similarly
Method obtains (B × Feng Legao lures No. 3) F3BC1 or (B × Feng Legao lures No. 3) F4BC1；Generally it is returned in F3 or F4 generation,
It need to specifically be determined according to single plant inductivity measurement result；
(4) (A × Feng Legao lures No. 3) F3BC1 or (A × Feng Legao lures No. 3) F4BC1, and (B × Feng Legao lures No. 3)
F3BC1 or (B × Feng Legao lures No. 3) F4BC1 carry out inbreeding of more generation, the use of haploid-induction test are F049 while selfing
The inductivity of measurement separation offspring, wherein the highest single plant of inductivity continues to add generation for selection, until separation offspring induces
Rate induces system up to 10% or more stablize；
(5) by induction system isolated in (A × Feng Legao lures No. 3) F3BC1 or (A × Feng Legao lures No. 3) F4BC1,
Isolated induction system is hybridized in (B × Feng Legao lures No. 3) F3BC1 or (B × Feng Legao lures No. 3) F4BC1
Type induction system, can be used as novel induction system for monoploid and is engineered breeding.
The beneficial effects of the present invention are embodied in:
The induction system that the present invention generates have well developed root system, stalk is sturdy, plant type is compact, resistance to close, anti-fall, resistant to high temperatures, colored
The advantage that powder amount is big, the loose powder time is long can effectively expand sizable application when carrying out monoploid engineering breeding, reduce risk.
Detailed description of the invention
Fig. 1 is the breeding flow diagram in the embodiment of the present invention 1.
Specific embodiment
It elaborates below to the embodiment of the present invention, the present embodiment carries out under the premise of the technical scheme of the present invention
Implement, the detailed implementation method and specific operation process are given, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
The present embodiment carries out breeding as follows:
1, No. 3 are lured with Feng Legao respectively with the maternal 192-4 and the prosperous 7-2 of male parent of the commercial hybrids kind Feng Le 33 of selection to assemble
The building form of F1, F1 are that 192-4 × Feng Legao lures No. 3 and prosperous 7-2 × Feng Legao lures No. 3, and when harvest pays attention to eliminating wherein embryo
The seed (monoploid seed) of the non-purple of point.Or reciprocal cross, i.e. Feng Legao lure No. 3 × 192-4 and Feng Legao to lure No. 3 × prosperous 7-2.
It lures No. 3 and prosperous 7-2 × Feng Legao to lure No. 3 to be selfed 192-4 × Feng Legao, obtains F2 seed, select wherein
Endosperm aleurone and embryo point are purple and the apparent seed of color.To guarantee that offspring can be separated to the higher single plant of inductivity,
Each F2 should pick out 40 or more for seed.The group's number the big more is conducive to reach target, but workload increases accordingly.
2, No. 3 and prosperous 7-2 × Feng Legao are lured to lure No. 3 F2 seed simple grain plantations 192-4 × Feng Legao of select；Together
When, plantation self-mating system F049 does test system, and strain number should be about 10 times of above-mentioned F2 seed.If each F2 is for seed plantation 40
Grain, two F2 are 80 for seed, then F049 kind plants 800 plants.
3, before (192-4 × Feng Legao lures No. 3) F2 and (prosperous 7-2 × Feng Legao lures No. 3) F2 takes out hero, it is green for can eliminating stalk
The plant of color only retains the plant that stalk is purple, single plant number, number are as follows: (192-4 × Feng Legao lures No. 3) F2-1,
(192-4 × Feng Legao lures No. 3) F2-2 ... ..., (192-4 × Feng Legao lures No. 3) F2-N；(prosperous 7-2 × Feng Legao lures No. 3)
F2-1, (prosperous 7-2 × Feng Legao lures No. 3) F2-2 ... ..., (prosperous 7-2 × Feng Legao lures No. 3) F2-N.
It 4, is F049 for female parent with test while each single plant of (192-4 × Feng Legao lures No. 3) F2 is selfed, with
Each single plant of (192-4 × Feng Legao lures No. 3) F2 is that male parent is hybridized, and 10 fringes of pollinating, when harvest counts each single plant and induces to obtain
Monoploid kernal number and total grain number, calculate single plant inductivity, the highest 5 F2 single plants of corresponding inductivity are picked out, from harvest
5 single fringes in respectively select wherein endosperm aleurone and embryo point is purple and the apparent seed of color 20, (192- is obtained
4 × Feng Legao lures No. 3) 100, F3 seed.
Meanwhile with test being F049 for female parent, hybridized using each single plant of (prosperous 7-2 × Feng Legao lures No. 3) F2 as male parent,
It pollinates 10 fringes, when harvest counts the monoploid kernal number and total grain number that each single plant induces, and calculates single plant inductivity, picks out
The corresponding highest 5 F2 single plants of inductivity respectively select wherein endosperm aleurone from 5 single fringes of harvest and embryo point are purple
And the apparent seed of color 20, it is obtained (prosperous 7-2 × Feng Legao lures No. 3) 100, F3 seed.
5,100, (192-4 × Feng Legao lures No. 3) F3 seed, (prosperous 7-2 × Feng Legao lures No. 3) F3 of select are planted
100, seed, test are about 2000 plants of F049, self-mating system 192-4 and each 20 plants of prosperous 7-2 or so.
6, before (192-4 × Feng Legao lures No. 3) F3 and (prosperous 7-2 × Feng Legao lures No. 3) F3 takes out hero, it is green for can eliminating stalk
The plant of color only retains the plant that stalk is purple, single plant number, number are as follows: (192-4 × Feng Legao lures No. 3) F3-1,
(192-4 × Feng Legao lures No. 3) F3-2 ... ..., (192-4 × Feng Legao lures No. 3) F3-N；(prosperous 7-2 × Feng Legao lures No. 3)
F3-1, (prosperous 7-2 × Feng Legao lures No. 3) F3-2 ... ... (prosperous 7-2 × Feng Legao lures No. 3) F3-N.
7, it is female parent with each single plant of (192-4 × Feng Legao lures No. 3) F3, is returned, obtains by male parent of self-mating system 192-4
(192-4 × Feng Legao lures No. 3) F3-1/192-4, (192-4 × Feng Legao lures No. 3) F3-2/192-4 ... ..., (192-4 × rich
Happy height lures No. 3) F3-N/192-4 seed.Meanwhile with test being F049 for female parent, with each list of (192-4 × Feng Legao lures No. 3) F3
Strain is that male parent is hybridized, and 10 fringes of pollinating, when harvest counts the monoploid kernal number and total grain number that each single plant induces, and is calculated
Single plant inductivity picks out the highest 5 F3 single plants of corresponding inductivity, respectively selects wherein endosperm paste from 5 single fringes of harvest
Bisque and embryo point are purple and the apparent seed of color 20, and (192-4 × Feng Legao lures No. 3) F3/192-4 seed is obtained
100.
Meanwhile with each single plant of (prosperous 7-2 × Feng Legao lures No. 3) F3 to be maternal, it is returned, obtains by male parent of the prosperous 7-2 of self-mating system
To (prosperous 7-2 × Feng Legao lures No. 3) the prosperous 7-2 of F3-1/, (prosperous 7-2 × Feng Legao lures No. 3) prosperous 7-2 ... ... of F3-2/, (prosperous 7-2 ×
Feng Legao lures No. 3) the prosperous 7-2 seed of F3-N/.It is F049 for female parent with test, with each single plant of (prosperous 7-2 × Feng Legao lures No. 3) F3
Being hybridized for male parent, 10 fringes of pollinating, the monoploid kernal number and total grain number that when harvest counts calculate single plant inductivity,
The highest 5 F3 single plants of corresponding inductivity are picked out, respectively select wherein endosperm aleurone and embryo point from 5 single fringes of harvest
For purple and the apparent seed of color 20, it is obtained (prosperous 7-2 × Feng Legao lures No. 3) F3/ prosperous 100,7-2 seed.
8, plant select 100, (192-4 × Feng Legao lures No. 3) F3/192-4 seed, (prosperous 7-2 × Feng Legao lures 3
Number) 100, the prosperous 7-2 seed of F3/, monoseeding；Meanwhile planting self-mating system F049 to do test is about 2000 plants of strain number.
It 9, can before the prosperous 7-2 of (192-4 × Feng Legao lures No. 3) F3/192-4 and (prosperous 7-2 × Feng Legao lures No. 3) F3/ takes out hero
The plant that stalk is green is eliminated, the plant that stalk is purple, single plant number, number are as follows: (192-4 × Feng Legao lures 3 are only retained
Number) F3/192-4-1, (192-4 × Feng Legao lures No. 3) F3/192-4-2 ... ..., (192-4 × Feng Legao lures No. 3) F3/192-
4-N；(prosperous 7-2 × Feng Legao lures No. 3) prosperous 7-2-1 of F3/, (prosperous 7-2 × Feng Legao lures No. 3) F3/ prosperous 7-2-2 ... ..., (prosperous 7-2
× Feng Legao lures No. 3) the prosperous 7-2-N of F3/.
It 10, is F049 for female parent with test while each individual plant selfing of (192-4 × Feng Legao lures No. 3) F3/192-4, with
Each single plant of (192-4 × Feng Legao lures No. 3) F3/192-4 is that male parent is hybridized, and 10 fringes of pollinating, when harvest counts each single plant and lures
The monoploid kernal number led and total grain number, calculate single plant inductivity, pick out highest 5 of corresponding inductivity (192-4 ×
Feng Legao lures No. 3) F3/192-4 single plant, wherein endosperm aleurone is respectively selected from 5 single fringes of harvest and embryo point is purple
And the apparent seed of color 20, (192-4 × Feng Legao lures No. 3) F3/192-4 selfed seed is obtained, symbolically is
(192-4 × Feng Legao lures No. 3) F3BC1F2, totally 100.
Meanwhile each individual plant selfing of (prosperous 7-2 × Feng Legao lures No. 3) the prosperous 7-2 of F3/, it is F049 for female parent with test, with (prosperous
7-2 × Feng Legao lures No. 3) each single plant of the prosperous 7-2 of F3/ is that male parent is hybridized, 10 fringes of pollinating, when harvest, counts each single plant and induces
The monoploid kernal number arrived and total grain number calculate single plant inductivity, pick out the highest 5 (prosperous 7-2 × Feng Le of corresponding inductivity
Height lures No. 3) the prosperous 7-2 single plant of F3/, wherein endosperm aleurone is respectively selected from 5 single fringes of harvest and embryo point is purple and face
The apparent seed of color 20, obtains the prosperous 7-2 selfed seed of (prosperous 7-2 × Feng Legao lures No. 3) F3/, and symbolically is (prosperous 7-2
× Feng Legao lures No. 3) F3B C1F2, totally 100.
11, plant select 100, (192-4 × Feng Legao lures No. 3) F3BC1F2 seed, (prosperous 7-2 × Feng Legao lures 3
Number) 100, F3BC1F2 seed, monoseeding；Meanwhile planting self-mating system F049 to do test is about 2000 plants of strain number.
It 12, can before (192-4 × Feng Legao lures No. 3) F3BC1F2 and (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F2 takes out hero
The plant that stalk is green is eliminated, the plant that stalk is purple, single plant number, number are as follows: (192-4 × Feng Legao lures 3 are only retained
Number) F3BC1F2-1, (192-4 × Feng Legao lures No. 3) F3BC1F2-2 ... ..., (192-4 × Feng Legao lures No. 3) F3BC1F2-
N；(prosperous 7-2 × Feng Legao lures No. 3) F3BC1F2-1, (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F2-2 ... ..., (prosperous 7-2 × rich
Happy height lures No. 3) F3BC1F2-N.
It 13, is F049 for female parent with test while each individual plant selfing of (192-4 × Feng Legao lures No. 3) F3BC1F2, with
Each single plant of (192-4 × Feng Legao lures No. 3) F3BC1F2 is that male parent is hybridized, 10 fringes of pollinating, and when harvest counts each single plant induction
Obtained monoploid kernal number and total grain number calculate single plant inductivity, pick out the highest 5 (192-4 × rich of corresponding inductivity
Happy height lures No. 3) F3BC1F2 single plant, wherein endosperm aleurone is respectively selected from 5 single fringes of harvest and embryo point is purple and face
The apparent seed of color 20 obtains (192-4 × Feng Legao lures No. 3) F3BC1F3 selfed seed totally 100.
Meanwhile each individual plant selfing of (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F2, it is F049 for female parent with test, with (prosperous 7-
2 × Feng Legao lures No. 3) each single plant of F3BC1F2 is that male parent is hybridized, 10 fringes of pollinating, when harvest, counts each single plant and induces to obtain
Monoploid kernal number and total grain number, calculate single plant inductivity, pick out the highest 5 (prosperous 7-2 × Feng Legao of corresponding inductivity
Lure No. 3) F3BC1F2 single plant, wherein endosperm aleurone is respectively selected from 5 single fringes of harvest and embryo point is purple and color is bright
Aobvious seed 20 obtains (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F3 selfed seed totally 100.
14, plant select 100, (192-4 × Feng Legao lures No. 3) F3BC1F3 seed, (prosperous 7-2 × Feng Legao lures 3
Number) 100, F3BC1F3 seed, monoseeding；Meanwhile planting self-mating system F049 to do test is about 2000 plants of strain number.
It 15, can before (192-4 × Feng Legao lures No. 3) F3BC1F3 and (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F3 takes out hero
The plant that stalk is green is eliminated, the plant that stalk is purple, single plant number, number are as follows: (192-4 × Feng Legao lures 3 are only retained
Number) F3BC1F3-1, (192-4 × Feng Legao lures No. 3) F3BC1F3-2 ... ..., (192-4 × Feng Legao lures No. 3) F3BC1F3-
N；(prosperous 7-2 × Feng Legao lures No. 3) F3BC1F3-1, (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F3-2 ... ..., (prosperous 7-2 × rich
Happy height lures No. 3) F3BC1F3-N.
It 16, is F049 for female parent with test while each individual plant selfing of (192-4 × Feng Legao lures No. 3) F3BC1F3, with
Each single plant of (192-4 × Feng Legao lures No. 3) F3BC1F3 is that male parent is hybridized, 10 fringes of pollinating, and when harvest counts each single plant induction
Obtained monoploid kernal number and total grain number calculate single plant inductivity, pick out the highest 5 (192-4 × rich of corresponding inductivity
Happy height lures No. 3) F3BC1F3 single plant, wherein endosperm aleurone is respectively selected from 5 single fringes of harvest and embryo point is purple and face
The apparent seed of color 20 obtains totally 100, (192-4 × Feng Legao lures No. 3) F3BC1F4 seed.
Meanwhile each individual plant selfing of (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F3, it is F049 for female parent with test, with (prosperous 7-
2 × Feng Legao lures No. 3) each single plant of F3BC1F3 is that male parent is hybridized, 10 fringes of pollinating, when harvest, counts each single plant and induces to obtain
Monoploid kernal number and total grain number, calculate single plant inductivity, pick out the highest 5 (prosperous 7-2 × Feng Legao of corresponding inductivity
Lure No. 3) F3BC1F3 single plant, wherein endosperm aleurone is respectively selected from 5 single fringes of harvest and embryo point is purple and color is bright
Aobvious seed 20 obtains totally 100, (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F4 seed.
17, (192-4 × Feng Legao lures No. 3) 100, F3BC1F4 seed of select is planted, (prosperous 7-2 × Feng Legao lures 3
Number) 100, F3BC1F4 seed, monoseeding；Meanwhile planting self-mating system F049 to do test is about 2000 plants of strain number.(192-
4 × Feng Legao lures No. 3) F3BC1F4 and (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F4 take out it is male before, it is green for can eliminating stalk
Plant only retains the plant that stalk is purple, single plant number, number are as follows: (192-4 × Feng Legao lures No. 3) F3BC1F4-1,
(192-4 × Feng Legao lures No. 3) F3BC1F4-2 ... ..., (192-4 × Feng Legao lures No. 3) F3BC1F4-N；(prosperous 7-2 × Feng Le
Height lures No. 3) F3BC1F4-1, (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F4-2 ... ..., (prosperous 7-2 × Feng Legao lures No. 3)
F3BC1F4-N。
It 18, is F049 for female parent with test while each individual plant selfing of (192-4 × Feng Legao lures No. 3) F3BC1F4, with
Each single plant of (192-4 × Feng Legao lures No. 3) F3BC1F4 is that male parent is hybridized, 10 fringes of pollinating, and when harvest counts each single plant induction
Obtained monoploid kernal number and total grain number calculate single plant inductivity, pick out the highest 5 (192-4 × rich of corresponding inductivity
Happy height lures No. 3) F3BC1F4 single plant, wherein endosperm aleurone is respectively selected from 5 single fringes of harvest and embryo point is purple and face
The apparent seed of color 20 obtains totally 100, (192-4 × Feng Legao lures No. 3) F3BC1F5 seed.
Meanwhile each individual plant selfing of (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F4, it is F049 for female parent with test, with (prosperous 7-
2 × Feng Legao lures No. 3) each single plant of F3BC1F4 is that male parent is hybridized, 10 fringes of pollinating, when harvest, counts each single plant and induces to obtain
Monoploid kernal number and total grain number, calculate single plant inductivity, pick out the highest 5 (prosperous 7-2 × Feng Legao of corresponding inductivity
Lure No. 3) F3BC1F4 single plant, wherein endosperm aleurone is respectively selected from 5 single fringes of harvest and embryo point is purple and color is bright
Aobvious seed 20 obtains totally 100, (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F5 seed.
19, (192-4 × Feng Legao lures No. 3) 100, F3BC1F5 seed of select is planted, (prosperous 7-2 × Feng Legao lures 3
Number) 100, F3BC1F5 seed, monoseeding；Meanwhile planting self-mating system F049 to do test is about 2000 plants of strain number.
It 20, can before (192-4 × Feng Legao lures No. 3) F3BC1F5 and (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F5 takes out hero
The plant that stalk is green is eliminated, the plant that stalk is purple, single plant number, number are as follows: (192-4 × Feng Legao lures 3 are only retained
Number) F3BC1F5-1, (192-4 × Feng Legao lures No. 3) F3BC1F5-2 ... ..., (192-4 × Feng Legao lures No. 3) F3BC1F5-
N；(prosperous 7-2 × Feng Legao lures No. 3) F3BC1F5-1, (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F5-2 ... ..., (prosperous 7-2 × rich
Happy height lures No. 3) F3BC1F5-N.
It 21, is F049 for female parent with test while each individual plant selfing of (192-4 × Feng Legao lures No. 3) F3BC1F5, with
Each single plant of (192-4 × Feng Legao lures No. 3) F3BC1F5 is that male parent is hybridized, 10 fringes of pollinating, and when harvest counts each single plant induction
Obtained monoploid kernal number and total grain number calculate single plant inductivity, pick out the highest (192-4 × Feng Le of corresponding inductivity
Height lures No. 3) F3BC1F5 single plant, whole fringe seed is harvested, obtains (192-4 × Feng Legao lures No. 3) F3BC1F6 seed, at this time seed
Basic neat and consistent no longer needs to select.
Meanwhile each individual plant selfing of (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F5, it is F049 for female parent with test, with (prosperous 7-
2 × Feng Legao lures No. 3) each single plant of F3BC1F5 is that male parent is hybridized, 10 fringes of pollinating, when harvest, counts each single plant and induces to obtain
Monoploid kernal number and total grain number, calculate single plant inductivity, picking out corresponding inductivity, highest (prosperous 7-2 × Feng Legao lures 3
Number) F3BC1F5 single plant, whole fringe seed is harvested, (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F6 seed is obtained, at this time seed base
This neat and consistent no longer needs to select.
22, plantation (192-4 × Feng Legao lures No. 3) F3BC1F6 and (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F6 seed,
At this point, (192-4 × Feng Legao lures No. 3) F3BC1F6 and (prosperous 7-2 × Feng Legao lures No. 3) F3BC1F6 can be used as stable selfing
System uses, and no longer needs to carry out inductivity measurement, when breeding need to be only selfed or sisters hand over.Induction is named as to be 192-4 and lure
Leading is prosperous 7-2.The use of induction is 192-4 and induction be induction that prosperous 7-2 is assembled be 192-4 × induction is prosperous 7-2, can make
For new and effective strong advantage induction system induction basic material Haploid production.
Due in Breeding Process, primary backcrossing only is carried out using 192-4 and prosperous 7-2, therefore inducing is 192-4 × lure
Leading is prosperous 7-2 compared with commercial hybrids kind 192-4 × prosperous 7-2, and plant forms have a larger difference, but as main using pollen
Induction system, advantage is sufficiently strong, and pollen amount is sufficiently large.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (3)
1. a kind of selection of corn haploid induction line, it is characterised in that: high-frequency induction system Feng Legao is selected to lure No. 3, choosing
With the commercial hybrids kind promoted in production, self-mating system F049 is selected to be haploid-induction test system；Utilize commercial hybrids kind
Parents' transformation induction system, transformation use hybridization, selfing, backcrossing means in the process, and single plant inductivity is combined to measure in the process,
Seed and plant color mark are selected；The induction of two kinds of good Different groups of transformation is A and induction is that B is hybridized again,
Induction system is done using Hybrid F1 generation.
2. selection according to claim 1, it is characterised in that: the commercial hybrids kind should have well developed root system, stem
Stalk is sturdy, plant type is compact, resistance to advantage close, anti-fall, resistant to high temperatures, pollen amount is big, the loose powder time is long；And the commercial hybrids kind
Maternal A and male parent B should not also have critical defect.
3. selection according to claim 1, which comprises the steps of:
(1) No. 3 are lured to assemble F1 with Feng Legao respectively with the maternal A of commercial hybrids kind and male parent B, the building form of F1 is A × rich
Happy height lures No. 3 and B × Feng Legao to lure No. 3 or reciprocal cross；
(2) A × Feng Legao lure No. 3 and B × Feng Legao lure No. 3 it is each carry out 2 generations or inbreeding of more generation, selfing while, uses monoploid
Inductivity test is that F049 measurement A × Feng Legao lures No. 3 and B × Feng Legao inductivities for luring No. 3 separation offsprings, and selection wherein lures
The highest single plant of conductance continues plus generation, until there is inductivity up to 10% or more single plant；
(3) from the separation offspring that A × Feng Legao lures No. 3, the highest single plant of inductivity is selected to do female parent from generation to generation suitable,
It is returned by male parent of A, obtains (A × Feng Legao lures No. 3) F3BC1 or (A × Feng Legao lures No. 3) F4BC1；Same method
Obtain (B × Feng Legao lures No. 3) F3BC1 or (B × Feng Legao lures No. 3) F4BC1；It is generally returned in F3 or F4 generation, specifically
It need to be determined according to single plant inductivity measurement result；
(4) (A × Feng Legao lures No. 3) F3BC1 or (A × Feng Legao lures No. 3) F4BC1, and (B × Feng Legao lures No. 3) F3BC1 or
(B × Feng Legao lures No. 3) F4BC1 carries out inbreeding of more generation, the use of haploid-induction test is F049 measurement point while selfing
Inductivity from offspring, wherein the highest single plant of inductivity continues to add generation for selection, until separation offspring inductivity occurs and reaches
10% or more stablize induces system；
(5) by induction system isolated in (A × Feng Legao lures No. 3) F3BC1 or (A × Feng Legao lures No. 3) F4BC1, and (B
× Feng Legao lures No. 3) induction system isolated in F3BC1 or (B × Feng Legao lures No. 3) F4BC1 hybridizes to obtain hybrid and lures
It leads and is, can be used as novel induction system for monoploid and be engineered breeding.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165350A (en) * | 2020-03-18 | 2020-05-19 | 中国农业大学 | Breeding method for efficiently identifying induction line of corn haploid immature embryo |
| CN113455378A (en) * | 2021-08-04 | 2021-10-01 | 北大荒垦丰种业股份有限公司 | Breeding method of corn haploid induction line and application thereof |
| CN113575412A (en) * | 2021-09-02 | 2021-11-02 | 河北冀农种业有限责任公司 | Breeding method of corn haploid induction line |
-
2019
- 2019-05-27 CN CN201910444056.9A patent/CN110089420A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111165350A (en) * | 2020-03-18 | 2020-05-19 | 中国农业大学 | Breeding method for efficiently identifying induction line of corn haploid immature embryo |
| CN111165350B (en) * | 2020-03-18 | 2021-10-22 | 中国农业大学 | Breeding method for efficiently identifying induction line of corn haploid immature embryo |
| CN113455378A (en) * | 2021-08-04 | 2021-10-01 | 北大荒垦丰种业股份有限公司 | Breeding method of corn haploid induction line and application thereof |
| CN115843674A (en) * | 2021-08-04 | 2023-03-28 | 北大荒垦丰种业股份有限公司 | Breeding method of corn haploid induction line and application thereof |
| CN113575412A (en) * | 2021-09-02 | 2021-11-02 | 河北冀农种业有限责任公司 | Breeding method of corn haploid induction line |
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Application publication date: 20190806 |