CN109362562A - Breeding method of cabbage type rape restorer line with orange yellow flower color marker character - Google Patents
Breeding method of cabbage type rape restorer line with orange yellow flower color marker character Download PDFInfo
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- CN109362562A CN109362562A CN201811597076.1A CN201811597076A CN109362562A CN 109362562 A CN109362562 A CN 109362562A CN 201811597076 A CN201811597076 A CN 201811597076A CN 109362562 A CN109362562 A CN 109362562A
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- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 title claims abstract description 51
- 240000007124 Brassica oleracea Species 0.000 title claims abstract description 47
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 title claims abstract description 47
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 title claims abstract description 47
- 238000009395 breeding Methods 0.000 title claims abstract description 20
- 239000003550 marker Substances 0.000 title abstract description 8
- 241000196324 Embryophyta Species 0.000 claims abstract description 65
- 230000001488 breeding effect Effects 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 19
- 238000012360 testing method Methods 0.000 claims abstract description 17
- 230000035558 fertility Effects 0.000 claims abstract description 5
- 241000723353 Chrysanthemum Species 0.000 claims description 26
- 235000007516 Chrysanthemum Nutrition 0.000 claims description 26
- 235000009127 Chrysanthemum hybrid Nutrition 0.000 claims description 11
- 230000008774 maternal effect Effects 0.000 claims description 9
- 230000000306 recurrent effect Effects 0.000 claims description 8
- 230000008569 process Effects 0.000 claims description 6
- 238000011084 recovery Methods 0.000 claims description 5
- 238000009396 hybridization Methods 0.000 claims description 3
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 claims description 3
- 238000012216 screening Methods 0.000 abstract description 2
- 241000207199 Citrus Species 0.000 abstract 1
- 241000756137 Hemerocallis Species 0.000 abstract 1
- 235000020971 citrus fruits Nutrition 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 description 10
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000009402 cross-breeding Methods 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 235000006008 Brassica napus var napus Nutrition 0.000 description 4
- 241001674939 Caulanthus Species 0.000 description 4
- 238000002955 isolation Methods 0.000 description 3
- 240000002791 Brassica napus Species 0.000 description 2
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 2
- 206010021929 Infertility male Diseases 0.000 description 2
- 208000007466 Male Infertility Diseases 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 2
- 244000088415 Raphanus sativus Species 0.000 description 2
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 238000009394 selective breeding Methods 0.000 description 2
- 241001582888 Lobus Species 0.000 description 1
- 101000708283 Oryza sativa subsp. indica Protein Rf1, mitochondrial Proteins 0.000 description 1
- 108700005079 Recessive Genes Proteins 0.000 description 1
- 102000052708 Recessive Genes Human genes 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 208000000509 infertility Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for breeding a cabbage type rape restorer line with a orange yellow flower color marker character, which utilizes the restorer line with the orange yellow flower color marker character, and a yellow flower hybrid plant is easy to identify and completely pull out from the content of the restorer line of orange yellow flower during blooming, and comprises the following steps: selecting yellow flower single plant of cabbage type rape restorer line as female parent, taking cabbage type rape self-bred line with orange yellow flower mark character as male parent, and hybridizing to obtain F1A yellow flower group; then selfing and backcrossing are carried out; proceed to the fifth backcross and the sixth selfing at BC5F2Selecting Citrus daylily single plant in the generation group, continuously selfing for three generations, performing test cross with sterile line matched with cabbage type rape restorer line, and screening F1The generation fertility rate reaches 100%, and the parent plant with strong heterosis and good comprehensive character is the cabbage type rape restorer with the orange yellow flower marker character.
Description
Technical field
The present invention relates to a kind of cabbage type rape Breeding for restoration lines methods, belong to crop breeding technical field.
Background technique
Term is explained:
Backcrossing: after two parents, first filial generation hybridizes again and again with its parent's, is referred to as returned.
Back cross breeding: using repeatedly backcrossing, using selfing, thus the method for being bred as new varieties.
Recurrent parent: the parent used is repeated as many times in back cross breeding.
Nonrecurrent parent: 1 parent is only used in back cross breeding when hybridizing first time.
BC1: backcrossing is primary.
BC1F1: it is returned a primary generation.
BCnF1: it is returned a generation for n times.
BC1F2: two primary generations of selfing after backcrossing is primary.
BCnF2: two primary generations are selfed after backcrossing n times.
Currently, the Rape-seed production in 80% or more China uses Hybrid.Wherein three incross rapeseed includes restoring
System's isolation breeding seed and restorer and sterile line hybrid breeding.Realize three line hybrid seed stable high yield, cross breeding seed
Purity must reach 85% or more.The cenospecies produced using three series mating, the reduction of purity necessarily lead to the decline of yield.It is miscellaneous
The every decline 1% of kind of purity is handed over, yield will decline 0.2% to 0.4%.Impure parent is the most direct of an influence purity of hybrid
Factor, such as restorer contain hybrid strain 1%, can lead to purity of hybrid decline 1%.In Production of Large Fields, the napus hybrid production of hybrid seeds
Parental purity should reach 99% or more.
In cabbage type rape breeding process, mark property can be used and distinguish breeding material, so that poor with ordinary cole
It is different obvious, after removal of impurities, it can achieve ideal parental purity.Currently, scientific research personnel, which uses some mark properties, cultivates breeding
Material, but these characters are affected by environment larger, are somewhat limited in practical applications.For example, using lobus cardiacus purple as mark
Memory shape is influenced by autumn, the decline of winter temperature, general rape can all change colour, and be not easy in seedling stage in production of hybrid seeds production process
It differentiates.It using blade wax powder-free as mark property, is difficult in Production of Large Fields, operation difficulty is high, and heavy workload is identifying
It is easy to retain false kind in the process.
Summary of the invention
The petal color of traditional all plant of restorer offspring is identical as parent, is yellow.When there is hybrid strain in offspring,
Hybrid strain is difficult to be identified, pull out.In order to overcome the mark property of cabbage type rape in the prior art unobvious, it is not easy to identify group
The defect of the purity of body.The present invention provides the cabbage type rape recoveries that a kind of character significantly has orange suit markings character
System selective breeding method.When hybrid strain occurs in restorer offspring, it will appear apparent poor morphology between hybrid strain and restorer homozygosis offspring
It is different, it is easily identified in florescence.
The present invention is realized by following technical proposals.A kind of cabbage type rape recovery with orange suit markings character
System selective breeding method, steps are as follows:
(1) select the chrysanthemum single plant (JJRfRf) of cabbage type rape restorer for female parent, to have orange suit markings
The cabbage type rape self-mating system (jjnn) of shape is male parent, and hybridization obtains F1For chrysanthemum group (JjRfn)；
(2) F to be obtained in step (1)1It is selfed for chrysanthemum single plant (JjRfn), obtains F2For group；
(3) F to be obtained in step (2)2For orange colored single plant (jjRfRf) is screened in group, selects and mark and infertility
There is the fertile orange colored single plant (jjRfRf) of fertility full recovery in filial generation after the maternal test cross of system；
(4) using the orange colored single plant (jjRfRf) that is obtained after select in step (3) as female parent (nonrecurrent parent)；
(5) with the chrysanthemum single plant of the orange colored single plant (jjRfRf) and cabbage type rape restorer that are obtained in step (4)
(JJRfRf) male parent (recurrent parent) carries out first time backcrossing, obtains BC1F1For chrysanthemum backcross population (JjRfRf)；
(6) BC to be obtained in step (5)1F1It is selfed for chrysanthemum backcross population (JjRfRf), obtains BC1F2For group.
(7) backcrossing and selfing process for repeating step (5) and (6) are carried out to the 5th backcrossing and the 6th selfing,
BC5F2For orange colored single plant (jjRfRf) is selected in group, it is continuously selfed three generations, and not with cabbage type rape restorer combo
Educating is carry out test cross, filters out F1For fertile rate up to 100%, and hybrid vigour is strong and the good male parent strain of Comprehensive Traits is band
There is the cabbage type rape restorer of orange colored mark property.
Further, the method for cabbage type rape restorer purity of the identification with orange suit markings character are as follows: (1) extensive
Again be orange colored parent's single plant (jj) self progeny be orange flower (jj), chrysanthemum hybrid strain (JJ) be chrysanthemum and chrysanthemum hybrid strain with
The filial generation of the orange colored parent of restorer is chrysanthemum (Jj), and (2) are easily identified in flowering stage, hybrid strain；In full-bloom stage, pull out
Chrysanthemum hybrid strain；(3) seed harvested from maternal plant after maturation is restorer seed.
The present invention utilizes the restorer of orange colored mark property, when blooming, restorer content of the chrysanthemum hybrid strain in orange flower
It is easy to identify, it can all pull out, the purity of restorer can be made to be increased to 100%.
Mark property has specificity, and for the character of single recessive gene control, character is stablized, not by environmental condition shadow
It rings, character is obvious, easily identifies.In restorer reproductive process, conducive to identifying and pulling out hybrid strain, prevent from mixing other seeds,
Ensure restorer seed purity.
During the cross breeding seed of Production of Large Fields kind, in florescence readily identified restorer and sterile line, at end
Florescence is easier thoroughly to cut off restorer, but not fallibility cut off pollination after sterile line, ensure cenospecies seed purity and
Yield.
Detailed description of the invention
Fig. 1 is the cabbage type rape Breeding for restoration lines flow chart with orange suit markings character.
Orange flower color gene type jj Huang flower color gene type JJ and Jj
The genotype RfRf of restorer restoring gene without restoring gene genotype nn.
Fig. 2 is identification restorer purity schematic diagram.
The orange flower color gene type jj of restorer
Restorer offspring's hybrid strain Huang flower color gene type J.
Fig. 3 is cabbage type rape restorer 283OR (left side) and orange pattern cabbage type rape restorer JH-OR (right side) in flower
The comparison of phase main inflorescence.
Specific embodiment
The embodiment of the present invention is described below in detail, examples of the embodiments are shown in the accompanying drawings, wherein from beginning to end
Same or similar label indicates same or similar element or element with the same or similar functions.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to is used to explain the present invention, and is not considered as limiting the invention.
Embodiment 1- breeding has the cabbage type rape restorer of orange suit markings character
Cabbage type rape restorer
Cabbage type rape restorer is the radish cytoplasmic male sterility of Jiangxi Academy of Agricultural Sciences Crop Institute's breeding
The restorer OR283 of system.
Cabbage type rape sterile line
Cabbage type rape sterile line is to grind with Jiangxi Academy of Agricultural Sciences crop of cabbage type rape restorer OR283 combo
Study carefully the sterile line OA11 of the radish cytoplasmic male sterility system of institute's breeding.
Cabbage type rape self-mating system
Cabbage type rape self-mating system with orange suit markings character is Jiangxi Academy of Agricultural Sciences Crop Institute's choosing
The cabbage type rape routine strain JH1 educated.
Orange flower color gene type jj Huang flower color gene type JJ and Jj
The genotype RfRf of restorer restoring gene without restoring gene genotype nn.
In conjunction with Fig. 1, process is as follows
(1) hybridize
In March, 2007 is female parent with 2 plants of cabbage type rape restorer chrysanthemum single plants, with 5 plants of orange colored JH1 for orange flower
Male parent (jjnn), hybridization, obtains 60 filial generation F1For chrysanthemum group (JjRfn).
(2) it is selfed for the first time
In May, 2007, by the F of harvest1Qinghai is taken to for hybrid seed adds generation.In July, 2007, selfing.In September, 2007,
From 20 plants of F1Selfing obtains F2Generation.
(3) test cross and for the first time backcrossing
In March, 2008, from 230 plants of F2For 50 plants of selected marker number orange colored single plants in group, it is denoted as JH-OR-F2(1 to
50).It is female parent with cabbage type rape sterile line using it as male parent, test cross obtains 50 parts of test cross F1Generation.Select and mark with not
Educate is that the fertile orange colored single plant (jjRfRf) of fertility full recovery occurs in filial generation after maternal test cross
Meanwhile with 50 plants of marker number orange colored F2Single plant is female parent, using 5 plants of cabbage type rape restorers as chrysanthemum father
This (JJRfRf) (recurrent parent), backcrossing, obtains 50 parts of 1 generation group BC of backcrossing1F1。
(4) it is selfed for second
The backcrossing 1 generation group BC of 100% fertility restorer is accredited as from 10 parts of test crosses in July, 20081F1Middle 10 plants of selection, from
It hands over, obtains BC1F2Generation.
(5) it is returned for second
In March, 2009, from BC1F2In generation, selects 10 plants of orange colored single plants as maternal (nonrecurrent parent), with 2 plants of Wild cabbage types
Rape restorer is chrysanthemum male parent (JJRfRf) (recurrent parent), carries out second and is returned, obtains 10 parts of 2 generation groups of backcrossing
BC2F1。
(6) third time is selfed
In July, 2009, from 2 generation group BC of backcrossing2F1Middle 10 plants of selection, selfing obtain BC2F2Generation.
(7) third time is returned
In March, 2010, from BC2F2In generation, selects 10 plants of orange colored single plants as maternal (nonrecurrent parent), with 2 plants of Wild cabbage types
Rape restorer is chrysanthemum male parent (JJRfRf) (recurrent parent), carries out third time backcrossing, obtains 10 parts of 3 generation groups of backcrossing
BC3F1。
(8) the 4th selfings
In July, 2010, from 3 generation group BC of backcrossing3F1Middle 10 plants of selection, selfing obtain BC3F2Generation.
(9) the 4th backcrossings
In March, 2011, from BC3F2In generation, selects 10 plants of orange colored single plants as maternal (nonrecurrent parent), with 2 plants of Wild cabbage types
Rape restorer is chrysanthemum male parent (JJRfRf) (recurrent parent), carries out the 4th backcrossing, obtains 10 parts of 4 generation groups of backcrossing
BC4F1。
(10) the 5th selfings
In July, 2011, from 4 generation group BC of backcrossing4F1Middle 10 plants of selection, selfing obtain BC4F2Generation.
(11) the 5th backcrossings
In March, 2012, from BC4F2In generation, selects 10 plants of orange colored single plants as maternal (nonrecurrent parent), with 2 plants of Wild cabbage types
Rape restorer is chrysanthemum male parent (JJRfRf) (recurrent parent), carries out the 5th backcrossing, obtains 10 parts of 5 generation groups of backcrossing
BC5F1。
(12) the 6th selfings
In July, 2012, from 5 generation group BC of backcrossing5F1Middle 10 plants of selection, selfing obtain BC5F2Generation.
(13) the 7th selfings
In March, 2013, from 5 generation group BC of backcrossing5F210 plants of orange colored single plants of middle selection, selfing obtain orange colored BC5F3
Generation.
(14) test cross and the 8th selfing
In July, 2013, from BC5F3For 10 plants of selected marker number orange colored single plants in group, it is denoted as JH-OR-BC5F3(1
To 10).It is female parent with cabbage type rape sterile line using it as male parent, test cross obtains 10 parts of test cross F1Generation.
Meanwhile with 10 plants of marker number orange colored BC5F3Single plant, selfing obtain 10 parts of orange colored BC5F4Generation.
(15) isolation breeding and the 9th selfing
Plant 10 parts of test cross F in October, 20131In generation, every part is planted 100 plants.Respectively with the nylon wire of 80 mesh before just spending
Account isolation breeding.Florescence investigates test cross F1For fertility and harvest time species test.
Plant 5 generation group BC of 10 parts of selfings5F4, every part is planted 100 plants, totally 10 strains, and each strain is distinguished before just spending
Breeding is isolated with the nylon wire account of 80 mesh.
Filter out test cross F1For fertile rate up to 100%, and hybrid vigour is strong and the good orange colored BC of Comprehensive Traits5F4Father
This strain is the cabbage type rape restorer JH-OR with orange colored mark property.Fig. 3 is cabbage type rape restorer 283OR
(left side) and orange pattern cabbage type rape restorer JH-OR (right side) compare in florescence main inflorescence.
In conjunction with Fig. 2, the method for cabbage type rape restorer purity of the evaluation and screening with orange suit markings character are as follows:
(1) orange colored parent's single plant (jj) self progeny of restorer is orange flower (jj), and chrysanthemum hybrid strain (JJ) is that chrysanthemum and chrysanthemum are miscellaneous
Strain and the filial generation of the orange colored parent of restorer are chrysanthemum (Jj), and (2) are easily identified in flowering stage, hybrid strain；In full-bloom stage,
Pull out chrysanthemum hybrid strain；(3) seed harvested from maternal plant after maturation is restorer seed.
Above disclosed is only a preferred embodiment of the present invention, cannot limit the power of the present invention with this certainly
Sharp range, those skilled in the art can understand all or part of the processes for realizing the above embodiment, and weighs according to the present invention
Benefit requires made equivalent variations, still belongs to the scope covered by the invention.
Claims (3)
1. a kind of cabbage type rape Breeding for restoration lines method with orange suit markings character, it is characterized in that steps are as follows:
(1) select the chrysanthemum single plant (JJRfRf) of cabbage type rape restorer for female parent, to have orange suit markings character
Cabbage type rape self-mating system (jjnn) is male parent, and hybridization obtains F1For chrysanthemum group (JjRfn)；
(2) F to be obtained in step (1)1It is selfed for chrysanthemum single plant (JjRfn), obtains F2For group；
(3) F to be obtained in step (2)2For orange colored single plant (jjRfRf) is screened in group, selects and mark and sterile line mother
There is the fertile orange colored single plant (jjRfRf) of fertility full recovery in filial generation after this test cross；
(4) using the orange colored single plant (jjRfRf) that is obtained after select in step (3) as female parent (nonrecurrent parent)；
(5) with the chrysanthemum single plant of the orange colored single plant (jjRfRf) and cabbage type rape restorer that are obtained in step (4)
(JJRfRf) male parent (recurrent parent) carries out first time backcrossing, obtains BC1F1For chrysanthemum backcross population (JjRfRf)；
(6) BC to be obtained in step (5)1F1It is selfed for chrysanthemum backcross population (JjRfRf), obtains BC1F2For group.
(7) backcrossing and selfing process for repeating step (5) and (6) are carried out to the 5th backcrossing and the 6th selfing, in BC5F2Generation
Select orange colored single plant (jjRfRf) in group, be continuously selfed three generations, and with the sterile line of cabbage type rape restorer combo into
Row test cross, filters out F1For fertile rate up to 100%, and hybrid vigour is strong and the good male parent strain of Comprehensive Traits is with orange
The cabbage type rape restorer of flower mark property.
2. having the cabbage type rape Breeding for restoration lines method of orange suit markings character, feature according to claim 1
It is: the method for cabbage type rape restorer purity of the identification with orange suit markings character are as follows: (1) the orange colored parent of restorer
Single plant (jj) self progeny is orange flower (jj), and chrysanthemum hybrid strain (JJ) is chrysanthemum and chrysanthemum hybrid strain and restorer is orange spends parent
This filial generation is chrysanthemum (Jj), and (2) are easily identified in flowering stage, hybrid strain；(3) in full-bloom stage, chrysanthemum hybrid strain is pulled out；At
The seed harvested from maternal plant after ripe is restorer seed.
3. having the cabbage type rape Breeding for restoration lines method of orange suit markings character, feature according to claim 1
Be: using the restorer of orange colored mark property, when blooming, chrysanthemum hybrid strain easily identifies in the restorer of orange flower, chrysanthemum
Hybrid strain is all pulled out, and the purity of restorer is made to be increased to 100%.
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| CN111034546A (en) * | 2019-12-23 | 2020-04-21 | 云南素麻生物科技有限公司 | Breeding method of industrial cannabis with high CBD content |
| CN111771716A (en) * | 2020-07-24 | 2020-10-16 | 江西省农业科学院作物研究所 | Crop genetic breeding method for efficiently utilizing heterosis |
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