CN104886147B - Broad spectrum activity corn parthenogenesis-inducing regulator - Google Patents
Broad spectrum activity corn parthenogenesis-inducing regulator Download PDFInfo
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Abstract
The present invention is a kind of broad spectrum activity corn parthenogenesis-inducing regulator, including three kinds of sub- agent individually prepared and be used in combination, each sub- agent are formulated by different formulations and dosage by corresponding method.This derivant is compared with current domestic similar derivant, with good broad spectrum activity, by being used in combination three seed forming agents, all kinds being induced or combined material can be achieved it is solid, and the kind for the derivant typically only 22 35% that the country uses at present or combined material can induce it is solid；And the induction setting percentage of derivant of the present invention is higher, average every solid number of fringe is much higher than common derivant averagely per the solid number of fringe 0.59 0.68 up to 1.0 1.5, therefore this derivant is one of derivant best in current domestic similar derivant.
Description
Technical field
The present invention relates to a kind of corn parthenogenesis-inducing regulator, specifically a kind of broad spectrum activity corn parthenogenesis-inducing regulator,
Belong to crop breeding technical field.
Background technology
Cenospecies is promoted, is that countries in the world development Maize Production and raising corn yield generally use using hybrid vigour
A most economical maximally effective measure.And the basis of seed selection cenospecies and crux are selecting and breeding corn self-mating systems.According to routine
Method Breeding of Inbred Lines, it need to can be typically bred as through six to eight generations continuous artificial bagging, selfing and selecting party, the time is very long, hand
Continuous cumbersome, the human and material resources and financial resources of consumption are also larger.And use parthenogenetic technology, that is, utilize artificial synthesized chemical drugs
Thing derivant sprays or is injected on the corn capillament without pollination, induces it to bear seed, then by 2-3 generations plantation, from
Hand over and identify, you can be bred as tens to hundreds of（Depending on inducing amount and effect）The corn selfing of character neat and consistent
System, improve the efficiency of selecting and breeding corn self-mating system with getting twice the result with half the effort.
Carry out this research earliest in China is Institute of Genetics, Academia Sinica, they by parthenogenetic technology,
The germ plasm resource of thousands of stabilization characteristics of genetics is being obtained not between long 15 years（Corn inbred line）, and reflected by combining
Surely something lost list 6, section beautiful No. 4, No. 5 No. 10, Qin Dan cenospecies are selected.Such speed and efficiency are other breeding methods institute nothings
Method analogy.The Chinese Academy of Sciences heredity this technology applied for national inventing patent in 1998（CN98117627.5 is utilized
Distant hybridization and the method for single-female generation production corn hybrid seed）.Under the influence of heredity institute of the Chinese Academy of Sciences, domestic many breedings
Unit has carried out rapidly this research, and for the moment into the new focus of corn breeding, but the derivant of constituent parts research and development at present is total to
It is with deficiency：1st, derivant does not possess broad spectrum activity, and general only 22-35% kind or combined material can induce solid.2nd, can lure
It is relatively low to lead solid kind induction setting percentage, the averagely every solid number of fringe of solid strain is through conversion only 0.59-0.68.
The content of the invention
It is an object of the invention to for vast corn breeding worker provide it is a kind of have broad spectrum activity and induction setting percentage it is high,
The corn parthenogenesis-inducing regulator of simple operation is induced, and it is desirable that by using derivant of the invention, can be createed for China
More abundant corn germ plasm resource and more corn improved Varieties are cultivated, so as to improve the yield of China's corn, promoted
Enter the development of China's Maize Production, preferably benefit the people.
The broad spectrum activity corn parthenogenesis-inducing regulator of the present invention includes the following three kinds sub- agent individually prepared and be used in combination,
The pharmaceutical formulation and dosage of each sub- agent are as follows：
Sub- agent A：(NH4)2SO4463mg/L, KNO32830mg/L, KH2PO4400mg/L, MgSO4·7H2O 185mg/
L, CaCl2·2H2O 166mg/L, Naa 12mg/L, Ze 6.0mg/L；
Sub- agent B：Fe2(SO4)2Solution 5.0ml/L, MnSO4·4H2O 22mg/L, ZnSO4·7H2O 8.6mg/L, H3BO3
6.0mg/L, KI 0.8mg/L, Na2MoO4·2H2O 0.25mg/L, CuSO4·5H2O 0.025mg/L, CoCl2·6H2O
0.025mg/L, inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride 0.4mg/L, pyridoxine hydrochloride 0.5mg/L,
Nicotinic acid 0.5mg/L, Naa 12mg/L, Ze 6.0mg/L；
Sub- agent C：(NH4)2SO4463mg/L, KNO32830mg/L, KH2PO4400mg/L, MgSO4·7H2O 185mg/
L, CaCl2·2H2O 166mg/L, MnSO4·4H2O 4.4mg/L, Fe2(SO4)2Solution 5.0ml/L, ZnSO4·7H2O
1.5mg/L, H3BO31.6mg/L, KI 0.8mg/L, glycine 2.0mg/L, thiamine hydrochloride 1.0mg/L, pyridoxine hydrochloride
0.5mg/L, nicotinic acid 0.5mg/L, Naa 12mg/L, Ze 6.0mg/L；
Fe in the sub- agent B and sub- agent C2(SO4)2Solution is 7.45g Na2-EDTA（Ethylenediamine tetra-acetic acid two is received）With
5.57g FeSO4·7H2O is dissolved in the solution of gained in 1000ml water.
The derivant used with the current country is compared, and is the advantages of broad spectrum activity corn parthenogenesis-inducing regulator of the invention：
1st, derivant of the present invention has good broad spectrum activity.The present invention is all to be induced by the way that three seed forming agents are used in combination
Kind or combined material can induce solid, and the derivant that uses of the country typically only has 22-35% kind or combination at present
Material can induce solid；
2nd, the induction setting percentage of derivant of the present invention is high.Averagely can per the solid number of fringe using the solid strain of derivant of the present invention
Up to 1.0-1.5, it is much higher than common derivant averagely per solid several 0.59-0.68 of fringe.
Embodiment
First, the preparation of derivant of the present invention
1st, the sub- agent A of corn parthenogenesis-inducing regulator of the present invention preparation steps and method are as follows：
（1）, weigh (NH respectively4)2SO4463mg, KNO32830mg, KH2PO4400mg, MgSO4·7H2O 185mg,
CaCl2·2H2O 166mg are put in 1000ml beakers, and the cold boiling water for pouring into about 800ml fully dissolves medicine；
（2）, weigh Naa 12mg and put in beaker, after fully being dissolved with a small amount of alcohol, then pour into step（1）Gained burns
In cup solution；
（3）, weigh Ze 6mg and put in beaker, after fully being dissolved with a small amount of HCl, then pour into step（2）Gained beaker
In solution；
（4）, pH value regulation：Testing procedure（3）The pH value of gained beaker solution, the pH value of solution is adjusted with HCl or NaOH
To 6.0；
（5）, constant volume：By step（4）Gained beaker solution adds sub- agent of the cold boiling water to 1000ml, as this derivant
A。
2nd, the sub- agent B of corn parthenogenesis-inducing regulator of the present invention preparation steps and method are as follows：
（1）, weigh MnSO respectively4·4H2O 22mg, ZnSO4·7H2O 8.6mg, H3BO36.0mg, KI 0.8mg,
Na2MoO4·2H2O 0.25mg, CuSO4·5H2O 0.025mg, CoCl2·6H2O 0.025mg are put in 1000ml beakers, then are measured
Take Fe2(SO4)2Solution 5.0ml is poured into this beaker, and the cold boiling water for pouring into about 800ml fully dissolves medicine, then is claimed respectively
Inositol 100mg, glycine 2.0mg, thiamine hydrochloride 0.4mg, pyridoxine hydrochloride 0.5mg, nicotinic acid 0.5mg is taken to pour into this
Dissolving is stirred in beaker solution；Wherein Fe2(SO4)2Solution is 7.45g Na2-EDTA（Ethylenediamine tetra-acetic acid two is received）With
5.57g FeSO4·7H2O is dissolved in the solution obtained by 1000ml water；
（2）, weigh Naa 12mg and put in beaker, after fully being dissolved with a small amount of alcohol, then pour into step（1）Gained burns
In cup solution；
（3）, weigh Ze 6mg and put in beaker, after fully being dissolved with a small amount of HCl, then pour into step（2）Gained beaker
In solution；
（4）, pH value regulation：Testing procedure（3）The pH value of gained beaker solution, the pH value of solution is adjusted with HCl or NaOH
To 6.0；
（5）, constant volume：By step（4）Gained beaker solution adds sub- agent of the cold boiling water to 1000ml, as this derivant
B。
3rd, the sub- agent C of corn parthenogenesis-inducing regulator of the present invention preparation steps and method are as follows：
（1）, weigh (NH respectively4)2SO4463mg, KNO32830mg, KH2PO4400mg, MgSO4·7H2O 185mg,
CaCl2·2H2O 166mg, MnSO4·4H2O 4.4mg, ZnSO4·7H2O 1.5mg, H3BO31.6mg, KI 0.8mg are put
In 1000ml beakers, then measure Fe2(SO4)2Solution 5.0ml is poured into this beaker, and pours into about 800ml cold boiling water by medicine
Fully dissolving, then glycine 2.0mg is weighed respectively, thiamine hydrochloride 1.0mg, pyridoxine hydrochloride 0.5mg, nicotinic acid 0.5mg
Pour into this beaker solution and be stirred dissolving；Wherein Fe2(SO4)2Solution is 7.45g Na2-EDTA（Ethylenediamine tetra-acetic acid two
Receive）With 5.57g FeSO4·7H2O is dissolved in the solution obtained by 1000ml water；
（2）, weigh Naa 12mg and put in beaker, after fully being dissolved with a small amount of alcohol, then pour into step（1）Gained burns
In cup solution；
（3）, weigh Ze 6mg and put in beaker, after fully being dissolved with a small amount of HCl, then pour into step（2）Gained beaker
In solution；
（4）, pH value regulation：Testing procedure（3）The pH value of gained beaker solution, the pH value of solution is adjusted with HCl or NaOH
To 6.0；
（5）, constant volume：By step（4）Gained beaker solution adds sub- agent of the cold boiling water to 1000ml, as this derivant
C。
2nd, the method that corn single-female generation breeding is carried out using derivant of the present invention
1st, the plantation of induced material
（1）, be available for induction material：May be used as the selecting and breeding corn selfing local varieties of based material, distant hybridses and
Cross combination etc. can be employed as the induced material of corn single-female generation, and because single-female generation can overcome, distant hybrid offspring's is serious
Separation, thus it is bigger to its meaning；
（2）, plantation strain number：It can be arranged according to itself strength and needs, induce each induced material more than number of combinations
Can be suitably less, on the contrary, can be suitably some more, general each material 15-25 strains；
（3）, planting season：Weather conditions during the inductivity of single-female generation and dispenser have close relationship, high temperature drying
Weather can significantly affect inducing effect, 32-34 degree is no more than with temperature, relative temperature is ideal not less than 75-80%, therefore,
Sowing time should be calculated using the weather conditions of tasseling stage as Main Basiss, Guangxi osmanthus ALFISOL IN CENTRAL spring with May in
Ten days, autumn with early and middle ten dayses in October take out it is male spin and induced preferably；
（4）, planting area：Individually kind is best in isolated area for induced material, also may be used without the kind of isolated area in non-isolated area
With.
2nd, the induction of single-female generation
（1）, emasculation bagging：When induced material takes out hero, tassel is all pulled out, bagging isolation is carried out before fruit ear spinning, with
Pollen-proof pollutes；
（2）, injection derivant：When the fruit ear spinning same day or forward and backward 1-2 days of spinning, in morning dew is dry or neighbouring jade
After during before rice loose powder or afternoon 5, Cluster bagging is removed, fruit ear napex 3-5cm bract is cut off together with filigree with scissors,
The syringe needle that advance suction has induction jizi for making dumplings agent A is dripped 1-2 drops physic liquors on visible filigree surface or injects filigree slightly
And 1-2 drops physic liquors are injected, then put paper bag and fix；Paper bag injection 1-2 drop induction jizi for making dumplings agent B decoction is untied within second day,
Then paper bag is put to fix；Paper bag injection 1-2 drop induction jizi for making dumplings agent C decoction is untied within 3rd day, paper bag is then put and fixes
It is good.The injection of above-mentioned three seed forming agents decoction can also be carried out every other day；
Injection derivant can also use the method for spraying derivant substitute, and decoction is accurately sprayed at into flower during sprinkling
On silk.Spraying surely enters in filigree because decoction differs, so its inducing effect is stable not as injection；
（3）, it is listed：Liquid medicine injection is followed by listed, indicates the combination title or code name and dispenser date of induced material；
（4）, after dispenser to induction plant filigree it is withered before, fruit ear should be checked 2-3 times, the filigree that will be stretched out outside bag is cut
Short, exposed to prevent filigree paper bag toward moving up when fruit ear elongation will break top, the powder that causes to go here and there mixes.
3rd, the harvest and statistics of single-female generation
During seed maturity, with induced material（Combination）It is that unit harvests respectively with solid strain, gained seed is lonely female life
Grow a generation（Pa1）Seed, and counted：
Combination setting percentage=（Solid number of combinations/induction number of combinations）×100%
The solid number of average every fringe=solid total grain number/induction spike number
4th, the plantation of single-female generation offspring
（1）, a single-female generation generation（Pa1）Plantation
A, sow：With induced material（Combination）Simple grain program request is carried out respectively for unit with the solid strain of induction, is taken out
Bagging and selfing are carried out when male respectively；
B, harvest and numbering：Harvest and be numbered during seed maturity.
（2）, the generation of single-female generation two（Pa2）Plantation
A, sow：Pa1It is Pa to withhold the seed obtained2In generation, to combine with units of solid strain during sowing, fringe is divided to be broadcast
Kind（Head progeny row garden）；
B, homozygosity is identified：Pa2Generation, which there are about 20%, homozygous to turn into self-mating system.Can be according to plant height, plant type, ripe phase, resistance
Etc. being determined；
C, bagging is selfed：It is identified performance neat and consistent meet self-mating system standard be self-mating system, can bagging selfing or
Sister is handed over and reserved seed for planting, and test cross is carried out with its pollen ahead of time as character is excellent, can root as trait expression not enough neatly differs greatly
Decided what to use according to whether itself strength and character are excellent；
D, number：Harvest and be numbered during seed maturity.
（3）, single-female generation three generations（Pa3）Plantation
A, sow：Pa2The seed obtained is withheld, head progeny row sowing is carried out in units of fringe（Head progeny row garden）；
B, homozygosity is identified：Pa3Generation there are about 80% head progeny row can homozygosis be self-mating system, it is identified to meet self-mating system standard
Be selfed or sister hand over reserve seed for planting, also can roguing or take its pollen carry out test cross, remaining eliminate or roguing selfing make further mirror
It is fixed.
3rd, derivant of the present invention carries out the specific implementation result of single-female generation induction
In autumn in 2001 to the autumn in 2002, I has been carried out lonely female using derivant of the present invention to the induced material of multiple kinds
Reproduction Induction experiments, specific statistical result such as following table.
Above-mentioned experiment and statistical result are published in《Guangxi Agricultural science》2003 the 6th interim, can by above-mentioned statistical result
See, derivant of the present invention has a good broad spectrum activity, and all kinds being induced can induce solid, not occur inducing
Solid kind, and the average single-strain fructification yield of derivant of the present invention is high, and the three of induction makes total solid number of average every fringe
Up to 1.52.
I is mainly independent research before 2008, and the first half of the year in 2007, Department of Agriculture of autonomous region found that I possesses this certainly
After the parthenogenetic technology of main intellectual property, attach great importance to, appropriation, which is given, at once supports, and indicates to provide with the germplasm of selecting and breeding corn
Source（Corn inbred line）Based on, it is desirable to corn inbred line 200 is selected in two years.Then taken the lead by me and set up cooperative groups reinforcement
Research, through great efforts, by 2008 only, i.e., corn inbred line more than 180 is selected altogether using the time of a year and a half, pass through material
Exchange from other places and introduce self-mating system more than 30,09 year first half of the year was entered to field in person by the organization leadership of Department of Agriculture of autonomous region and expert
Row is inspected for acceptance and confirmed.Up to the present, by using this technology, oneself selects single-female generation self-mating system more than 800.Profit
Cenospecies, which is assembled, with these self-mating systems sends out six corn screen selectings for combining participation autonomous regions experiments, ginseng altogether in 2010-2012
Unit is tried in addition to there is the scientific research institutions of strength, seeds company inside and outside area, also the U.S., India, Vietnam, Thailand, Australia,
The major company of the states such as Philippine, combination more than 140 of participating in the experiment every year, and it is selected to share 4 combinations in six combinations that we send out
Tried into area, wherein it is respectively the 1st, 2,5 there are three combination yield rankings.
Claims (5)
1. a kind of broad spectrum activity corn parthenogenesis-inducing regulator, including the following three kinds sub- agent individually prepared and be used in combination, each son
The pharmaceutical formulation and dosage of agent are as follows：
Sub- agent A：(NH4)2SO4463mg/L, KNO32830mg/L, KH2PO4400mg/L, MgSO4·7H2O 185mg/L,
CaCl2·2H2O 166mg/L, Naa 12mg/L, Ze 6.0mg/L；
Sub- agent B：Fe2(SO4)2Solution 5.0ml/L, MnSO4·4H2O 22mg/L, ZnSO4·7H2O 8.6mg/L, H3BO3
6.0mg/L, KI 0.8mg/L, Na2MoO4·2H2O 0.25mg/L, CuSO4·5H2O 0.025mg/L, CoCl2·6H2O
0.025mg/L, inositol 100mg/L, glycine 2.0mg/L, thiamine hydrochloride 0.4mg/L, pyridoxine hydrochloride 0.5mg/L,
Nicotinic acid 0.5mg/L, Naa 12mg/L, Ze 6.0mg/L；
Sub- agent C：(NH4)2SO4463mg/L, KNO32830mg/L, KH2PO4400mg/L, MgSO4·7H2O 185mg/L,
CaCl2·2H2O 166mg/L, MnSO4·4H2O 4.4mg/L, Fe2(SO4)2Solution 5.0ml/L, ZnSO4·7H2O 1.5mg/
L, H3BO31.6mg/L, KI 0.8mg/L, glycine 2.0mg/L, thiamine hydrochloride 1.0mg/L, pyridoxine hydrochloride 0.5mg/
L, nicotinic acid 0.5mg/L, Naa 12mg/L, Ze 6.0mg/L；
Fe in the sub- agent B and sub- agent C2(SO4)2Solution is 7.45g Na2- EDTA and 5.57g FeSO4·7H2O is dissolved in
The solution of gained in 1000ml water.
2. corn parthenogenesis-inducing regulator according to claim 1, it is characterized in that：The compound method of the sub- agent A includes
Following steps：
（1）, weigh (NH respectively4)2SO4463mg, KNO32830mg, KH2PO4400mg, MgSO4·7H2O 185mg,
CaCl2·2H2O 166mg are put in 1000ml beakers, and the cold boiling water for pouring into about 800ml fully dissolves medicine；
（2）, weigh Naa 12mg and put in beaker, after fully being dissolved with a small amount of alcohol, then pour into step（1）Gained beaker is molten
In liquid；
（3）, weigh Ze 6mg and put in beaker, after fully being dissolved with a small amount of HCl, then pour into step（2）Gained beaker solution
In；
（4）, pH value regulation：Testing procedure（3）The pH value of gained beaker solution, with HCl or NaOH adjust the pH value of solution to
6.0；
（5）, constant volume：By step（4）Gained beaker solution adds cold boiling water to 1000ml, as this derivant agent A.
3. corn parthenogenesis-inducing regulator according to claim 1, it is characterized in that：The compound method of the sub- agent B includes
Following steps：
（1）, weigh MnSO respectively4·4H2O 22mg, ZnSO4·7H2O 8.6mg, H3BO36.0mg, KI 0.8mg,
Na2MoO4·2H2O 0.25mg, CuSO4·5H2O 0.025mg, CoCl2·6H2O 0.025mg are put in 1000ml beakers, then are measured
Take Fe2(SO4)2Solution 5.0ml is poured into this beaker, and the cold boiling water for pouring into about 800ml fully dissolves medicine, then is claimed respectively
Inositol 100mg, glycine 2.0mg, thiamine hydrochloride 0.4mg, pyridoxine hydrochloride 0.5mg, nicotinic acid 0.5mg is taken to pour into this
Dissolving is stirred in beaker solution；Wherein Fe2(SO4)2Solution is 7.45g Na2- EDTA and 5.57g FeSO4·7H2O is molten
Solution obtained by water in 1000ml；
（2）, weigh Naa 12mg and put in beaker, after fully being dissolved with a small amount of alcohol, then pour into step（1）Gained beaker is molten
In liquid；
（3）, weigh Ze 6mg and put in beaker, after fully being dissolved with a small amount of HCl, then pour into step（2）Gained beaker solution
In；
（4）, pH value regulation：Testing procedure（3）The pH value of gained beaker solution, with HCl or NaOH adjust the pH value of solution to
6.0；
（5）, constant volume：By step（4）Gained beaker solution adds cold boiling water to 1000ml, as this derivant agent B.
4. corn parthenogenesis-inducing regulator according to claim 1, it is characterized in that：The compound method of the sub- agent C includes
Following steps：
（1）, weigh (NH respectively4)2SO4463mg, KNO32830mg, KH2PO4400mg, MgSO4·7H2O 185mg,
CaCl2·2H2O 166mg, MnSO4·4H2O 4.4mg, ZnSO4·7H2O 1.5mg, H3BO31.6mg, KI 0.8mg are put
In 1000ml beakers, then measure Fe2(SO4)2Solution 5.0ml is poured into this beaker, and pours into about 800ml cold boiling water by medicine
Fully dissolving, then glycine 2.0mg is weighed respectively, thiamine hydrochloride 1.0mg, pyridoxine hydrochloride 0.5mg, nicotinic acid 0.5mg
Pour into this beaker solution and be stirred dissolving；Wherein Fe2(SO4)2Solution is 7.45g Na2- EDTA and 5.57g FeSO4·
7H2O is dissolved in the solution obtained by 1000ml water；
（2）, weigh Naa 12mg and put in beaker, after fully being dissolved with a small amount of alcohol, then pour into step（1）Gained beaker is molten
In liquid；
（3）, weigh Ze 6mg and put in beaker, after fully being dissolved with a small amount of HCl, then pour into step（2）Gained beaker solution
In；
（4）, pH value regulation：Testing procedure（3）The pH value of gained beaker solution, with HCl or NaOH adjust the pH value of solution to
6.0；
（5）, constant volume：By step（4）Gained beaker solution adds cold boiling water to 1000ml, as this derivant agent C.
5. a kind of method that corn single-female generation breeding is carried out using corn parthenogenesis-inducing regulator as claimed in claim 1,
The plantation four of the induction of plantation, single-female generation, the harvest of single-female generation and statistics and single-female generation offspring including induced material
The individual stage, it is characterised in that the induction period of the single-female generation comprises the following steps：
（1）, emasculation bagging：When induced material takes out hero, tassel is all pulled out, bagging isolation is carried out before fruit ear spinning, to prevent flower
Powder pollutes；
（2）, injection derivant：It is not dry or neighbouring corn dissipates in the morning dew when fruit ear spinning same day or forward and backward 1-2 days of spinning
After during before powder or afternoon 5, Cluster bagging is removed, fruit ear napex 3-5cm bract is cut off together with filigree with scissors, pre-
The syringe needle that first inhaling has induction jizi for making dumplings agent A drips 1-2 drops physic liquors or pico- reeve filigree on visible filigree surface and noted
Enter 1-2 drops physic liquors, then put paper bag and fix；Paper bag injection 1-2 drop induction jizi for making dumplings agent B decoction is untied within second day, then
Paper bag is put to fix；Paper bag injection 1-2 drop induction jizi for making dumplings agent C decoction is untied within 3rd day, paper bag is then put and fixes；
（3）, it is listed：Liquid medicine injection is followed by listed, indicates the combination title or code name and dispenser date of induced material；
（4）, after dispenser to induction plant filigree it is withered before, fruit ear should be checked 2-3 times, the filigree that will be stretched out outside bag is cut short,
Exposed to prevent filigree paper bag toward moving up when fruit ear elongation will break top, the powder that causes to go here and there mixes.
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Citations (4)
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|---|---|---|---|---|
| CN1245636A (en) * | 1998-08-24 | 2000-03-01 | 中国科学院遗传研究所 | Method for producing corn hybrid seed by utilizing distant hybridization and parthenogenetic reproduction |
| CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
| CN102960242A (en) * | 2012-12-17 | 2013-03-13 | 沈阳金色谷特种玉米有限公司 | Breeding method for inducing parthenogenesis of corn by propyzamide |
| CN102550399B (en) * | 2011-12-22 | 2013-10-02 | 沈阳农业大学 | Breeding method for inducing parthenogenesis of corn by trifluralin |
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2015
- 2015-06-04 CN CN201510303742.6A patent/CN104886147B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1245636A (en) * | 1998-08-24 | 2000-03-01 | 中国科学院遗传研究所 | Method for producing corn hybrid seed by utilizing distant hybridization and parthenogenetic reproduction |
| CN102440179A (en) * | 2011-09-28 | 2012-05-09 | 广西壮族自治区玉米研究所 | Breeding method of maize parthenogenesis inducer and its application in maize inbred line breeding |
| CN102550399B (en) * | 2011-12-22 | 2013-10-02 | 沈阳农业大学 | Breeding method for inducing parthenogenesis of corn by trifluralin |
| CN102960242A (en) * | 2012-12-17 | 2013-03-13 | 沈阳金色谷特种玉米有限公司 | Breeding method for inducing parthenogenesis of corn by propyzamide |
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| 化学诱导玉米孤雌生殖的研究;涂升斌等;《四川农业大学学报》;19940930;第12卷(第3期);第423-430页；第423页摘要、第3-4段，第425页第1段，第426页第6段，第427页第5段 * |
| 玉米自交系快速高效育成法——孤雌生殖法;杨宪民;《广西农业科学》;20031130(第6期);第27-29页；第28页左栏第2-3段，第29页右栏第2段 * |
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