CN105850723A - Corn hybrid seed production method - Google Patents

Abstract

The invention discloses a corn hybrid seed production method. The method comprises the steps that a selfing line H96-72 is used as a female parent, and a selfing line L408 is used as a male parent, and corn hybrid seeds Ziyu 22 are obtained in a hybrid mode. The Ziyu 22 obtained through the method is high in yield, good in stability, good in quality, high in resistance and wide in application range.

Description

A kind of method of hybrid maize seed production

Technical field

The invention belongs to hybridization field, a kind of method being specifically related to hybrid maize seed production.

Background technology

Sichuan corn germ plasm resource is narrow, the selfing line selected and cenospecies can not be the most fine in character, in yield, on adaptability, in order to meet China's food and the needs of corn deep processing product, select high yield, the top priority that high anti-, wide adaptability, yield stability are good, quality better corn variety is we breeder.

China from the eighties successively from abroad introducing and applying external Semen Maydis excellent resources many, the corn breeding and the large-area corn yield that make China just have a new breakthrough, the corn breeder of China has successively selected large quantities of superior hybrid crosses from external introduction of use Foreign Germplasms Used, such as middle list 2, Danyu series, tuck in the money jade series etc. in single series, agricultural university's series, Zheng's single series, Sichuan, increase income for the corn yield increasing of China and made the biggest contribution.

Accordingly, it would be desirable to constantly transform, create the product that more performance is more excellent, to meet market demand.

Summary of the invention

The method that it is an object of the present invention to provide the more excellent hybrid maize seed production of a kind of performance.

A kind of method that the technical scheme is that hybrid maize seed production, utilizes selfing line H96-72 for female parent, is that male parent hybridizes with selfing line L408, and preparation obtains corn hybrid seed money beautiful 22.

Further, after selfing line H96-72 is the pollen results F1 generation awarding Shanxi 1572 with the H96 variant of the U.S.'s auspicious moral blood relationship, then the H96 variant pollen that backcrosses, material based on this after results seed, the excellent selfing of selecting through 8 generations is isozygotied incubation.

Further, selfing line L408 is red by U.S.'s blood relationship 18599() after the hybridization of × 543 × Thailand torrid zone blood relationship LS08 excellent selfing of selecting through 8 generations obtain.

Further, spatial separation or space-time isolation or barrier isolation is used during hybrid seeding, use spatial separation to require around production of hybrid seeds district and do not plant other Semen Maydis for more than 300 meters, sowing time in employing time insulation request production of hybrid seeds district will be with other more than 20 days Semen Maydis mistake phases, and employing barrier isolation refers to use village, house, tree is gloomy or hilltop barrier is isolated.

Further, during hybrid seeding, need Parent flowering regulation, first broadcast 1 phase male parent 50%, broadcast second phase male parent 50% every 5 days, after a phase male parent goes out pin, broadcast female parent.

Further, need maternal emasculation during hybrid seeding, use the method touching bud emasculation, take out maternal tassel the most thoroughly.

Further, after male parent pollination terminates, male parent all need to be rooted out.

Biomaterial H96-72, the L408 that the present invention relates to is the most disclosed in the variety certification material of non-patent literature, and above-mentioned biomaterial all has preservation at application inventor.Applicant ensure have rise present patent application day 20 years in the public provide.

The present invention utilizes selfing line H96-72 for female parent, and selfing line L408 is that male parent assembles and obtains corn hybrid seed money beautiful 22, and its feature is: period of duration: in the 134 days time of infertility of Mountainous Areas of Si Sichuan, plant height 258 centimetres, Ear height 121.2 centimetres, individual plant total leaf number 19-20 sheet, fruit ear tubular, average 20.2 centimetres of spike length, tassel row number 18 row, several 39 of row grain, 100-grain weight 30.1 grams, cob white, grain type partly becomes Dens Equi, yellow, seed-producing rate 85%.

Through Institute of Plant Protection's inoculated identification: Resistance To Helminthosporium Turcicum, in anti-helminthosporium maydis, feel banded sclerotial blight, through Quality Detection, crude protein 9.1%, crude fat 4.20%, Crude starch 72.9%, lysine 0.24%, grain test weight 762g/L.Purity Field 100%.

The feature of maternal self bred inbred line H96-72: period of duration about 113 days, seedling sheath purple, total leaf number 18-19 sheet, plant height 180 centimetres; 80 centimetres, fringe position, plant type is open and flat, and tassel divides skill 5-8, sterile lemma purple; filigree yellow, female Honoka silk is green, fruit ear cartridge type, spike length 18 centimetres; thick 4.7 centimetres of fringe, head progeny row 16-18 row, several 39 of row grain; 100-grain weight 28g, seed yellow, half solids; cob white, solid full point, seed-producing rate 85.5%.

Male parent selfing line L408 feature: period of duration is sowed in spring 115 days, plant height 200 centimetres, Ear height 80 centimetres, and plant type half is compact; cane " it " peace type, hence it is evident that, seedling stage cane purple, tassel branch 4-8; sterile lemma is green, and female Honoka silk is green, the long cartridge type of fruit ear; spike length 18 centimetres, thick 4.6 centimetres of fringe, head progeny row 16 row; several 33 of row grain, 100-grain weight 22g, seed yellow; Hard grain type, cob white, seed-producing rate 84%.

The present invention compared with prior art has the advantage that

1, yield high and stable production is good: within 2010, participate in Sichuan Province's mountain test: average yield per mu 548kg, ratio compares single 15 volume increase 12.9% in river；Within 2011, participate in the continuous examination of Mountainous Areas of Si Sichuan group, average yield per mu 556.6kg, 15 volume increase 9.9% more single than comparison river.20 points over 2 years of participating in the experiment are secondary, some point volume increase.

2, best in quality: outward appearance commodity is good, cutin content is high, through Quality Detection: grain test weight is 762g/L, crude protein 9.1%, crude fat 4.2%, Crude starch 72.9%, lysine 0.24%.

3, resistance: identify through sowing: anti-large and small pinta, anti-banded sclerotial blight, ear rot, Maize Ear Rot.

4, adaptability: being suitable for mountain area, hills plantation, spring and summer is broadcast can.

Accompanying drawing explanation

Fig. 1 is inbred line breeding production of hybrid seeds collection of illustrative plates of the present invention.

Detailed description of the invention

Embodiment 1: the tool of corn hybrid seed of the present invention money beautiful 22 stops Breeding Process

Money beautiful 22 is that prosperous ten thousand, the Sichuan sub-Science and Technology Ltd. of seed makees female parent with selfing line H96-72 in 2007, male parent is made with selfing line L408, hybridization assembles incubation, after wherein selfing line H96-72 is the pollen results F1 generation awarding Shanxi 1572 with the H96 variant of the U.S.'s auspicious moral blood relationship, backcross H96 variant pollen again, material based on this after results seed, the excellent selfing of selecting through 8 generations is isozygotied incubation.Male parent selfing line L408 is red by U.S.'s blood relationship 18599() after the hybridization of × 543 × Thailand torrid zone blood relationship LS08 excellent selfing of selecting through 8 generations obtain.

1, yield performance: beautiful 22 cenospecies of money assembled with H96-72 × L408, participated in the group test of mountain area, Sichuan Province, average yield per mu 548 kilograms, 15 volume increase 12.9% more single than comparison river in 2010;Within 2011, participate in the continuous examination of mountain area, Sichuan Province group, average yield per mu 556.6 kilograms, 15 volume increase 9.9% more single than comparison river.Volume increase point accounts for 100% participated in the experiment a little.

2, disease resistance

2011 with province's Institute of Plant Protection's inoculated identification: Resistance To Helminthosporium Turcicum, in anti-helminthosporium maydis, anti-ear rot, sense banded sclerotial blight.

3, grain quality is good

Through Quality Detection: grain test weight is 762g/L, crude protein 9.1%, crude fat 4.2%, Crude starch 72.9%, lysine 0.24%,

Embodiment 2: the seeding technique of cenospecies of the present invention money beautiful 22

1, security isolation, is strictly on guard against flyings string powder: must not plant other Semen Maydis within 300 around seed farm.Must be more than 25 days as used the time to isolate.

2, regulation father, maternal sowing gap stage: first broadcast a phase male parent 50%, broadcast second phase male parent 50% every 5 days, broadcast female parent when waiting phase male parent one leaf wholeheartedly.

3, Suitable Density and row ratio: broadcast 4000-4500 plant/acre for maternal every mu, male parent 1000 plants/acre, father, maternal row compare 1:5.

4, emasculation is rogued: seedling stage, jointing stage, bloom reel off raw silk from cocoons before strictly remove the hybrid strain in father, female row, variant and small and weak strain.Threshing ground, the miscellaneous fruit ear of roguing blanking is done well after results.

5, remove female parent male flower in time neatly, scalp male parent, in time, totally remove female parent male flower up hill and dale, be the key link preventing maternal selfing guarantee seed purity, it is necessary to use the way touching bud emasculation, the maternal male flower of removal up hill and dale timely, clean.Whole male parent is rooted out, in order to female parent growth, seed production height after male parent loose powder.

6, anti-pest and disease damage of curing: note having prevented aphid, red spider and the pest and disease damage such as Pyrausta nubilalis (Hubern)., banded sclerotial blight.

7, gather in the crops in time, keep properly: to gather in the crops air dry, timely parasite killing when seed maturity in time, prevent freezing going mouldy, affect germination percentage, threshing, during keeping, be strictly on guard against mechanical admixture.

Implement 3: cenospecies of the present invention provides the cultivation technique of beautiful 22

1, in good time early sowing, rational close planting: during money beautiful 22 belongs to, evening belongs to kind, suitably sows in spring, it is preferred with the sowing of 3 months middle or small ten days to early Aprils in Sichuan, density is planted with every mu of 2800-3200 and is advisable.

2, enhancing field management, enrich rich water, this variety yield height should be enhanced field management, and notes enriching rich water.

Embodiment described above only have expressed the detailed description of the invention of the application, and it describes more concrete and detailed, but therefore can not be interpreted as the restriction to the application protection domain.It should be pointed out that, for the person of ordinary skill of the art, on the premise of conceiving without departing from technical scheme, it is also possible to making some deformation and improvement, these broadly fall into the protection domain of the application.

Claims (7)

1. the method for a hybrid maize seed production, it is characterised in that utilize selfing line H96-72 for female parent, be that male parent hybridizes with selfing line L408, preparation obtains corn hybrid seed money beautiful 22.

The method of a kind of hybrid maize seed production the most according to claim 1, it is characterized in that, after selfing line H96-72 is the pollen results F1 generation awarding Shanxi 1572 with the H96 variant of the U.S.'s auspicious moral blood relationship, backcross H96 variant pollen again, material based on this after results seed, the excellent selfing of selecting through 8 generations is isozygotied incubation.

The method of a kind of hybrid maize seed production the most according to claim 1, it is characterised in that selfing line L408 is red by U.S.'s blood relationship 18599() after the hybridization of × 543 × Thailand torrid zone blood relationship LS08 excellent selfing of selecting through 8 generations obtain.

The method of a kind of hybrid maize seed production the most according to claim 1, it is characterized in that, spatial separation or space-time isolation or barrier isolation is used during hybrid seeding, use spatial separation to require around production of hybrid seeds district and do not plant other Semen Maydis for more than 300 meters, sowing time in employing time insulation request production of hybrid seeds district will be with other more than 20 days Semen Maydis mistake phases, and employing barrier isolation refers to use village, house, tree is gloomy or hilltop barrier is isolated.

The method of a kind of hybrid maize seed production the most according to claim 1, it is characterised in that need Parent flowering regulation during hybrid seeding, first broadcasts 1 phase male parent 50%, broadcast second phase male parent 50% every 5 days, broadcasts female parent after a phase male parent goes out pin.

The method of a kind of hybrid maize seed production the most according to claim 1, it is characterised in that need maternal emasculation during hybrid seeding, use the method touching bud emasculation, takes out maternal tassel the most thoroughly.

The method of a kind of hybrid maize seed production the most according to claim 1, it is characterised in that after male parent pollination terminates, male parent all need to be rooted out.