CN102150642B - Cockscomb new variety breeding method by utilizing distant hybridization and back cross breeding technology - Google Patents
Cockscomb new variety breeding method by utilizing distant hybridization and back cross breeding technology Download PDFInfo
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- CN102150642B CN102150642B CN 201110078292 CN201110078292A CN102150642B CN 102150642 B CN102150642 B CN 102150642B CN 201110078292 CN201110078292 CN 201110078292 CN 201110078292 A CN201110078292 A CN 201110078292A CN 102150642 B CN102150642 B CN 102150642B
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Abstract
The invention provides a method for breeding cockscomb new variety with new variety characteristics and diseases resistance, and the method is realized by utilizing a distant hybridization and back cross breeding technology of culturing cockscomb and feather cockscomb. The technical scheme of the method is as follows: a, selecting a distant parent to perform hybridization and hormone treatment; b, selecting a hybrid to perform back cross breeding with the other culture variety; c, selecting hybrid progeny; and d, stabilizing a finished product variety (system). The method has the following advantages that 1, excellent characteristics of wild feather cockscomb are hybridized to the new variety to form a poly-spike new variety, namely poly-spike red cockscomb; 2, new characteristics combining the cockscomb and the feather cockscomb are generated, such as red tower cockscomb, yellow tower cockscomb, red torch cockscomb and short multi-crown cockscomb; and 3, the new variety (system) has new ornamental characteristics, and has the characteristic of stronger disease resistance.
Description
Technical field
The present invention relates to a kind of method of utilizing distant hybridization and backcrossing technology seed selection cockscomb new varieties, belong to the technical field of the flower breeding of modern agriculture.
Background technology
In the process of cultivating improved seeds because easily success of intervarietal cross in the species, breeding men always mainly utilize genetic resources in the cultivated species (Pan Jiaju, 1994, the plant breeding introduction, 129-148).Now felt that the genetic resources in the cultivated species is poor, some feature, characteristic have been difficult to find in cultivar, and wild species generally exactly keep under harsh competition for existence and natural conditions and accumulated these features and characteristic.Therefore, excavate by the interspecific cross between cultivated species and the wild species and to have tamed and a large amount of still good genetic resourcess of unacclimated sibling species, become one of plant breeding very important field of research (Meng Jinling, plant reproductive genetics, 1995,296-357).Cockscomb and feather cockscomb are to belong to the kind that feather cockscomb belongs to two nearly edge of (Celosia).Long-term artificial cultivation and selection make cockscomb have inflorescence and become crown, the feature that stem is obvious, branch is few, blade is long and wide etc. is suitable for viewing and admiring, but it is higher to lack the plant of feather cockscomb, the feature such as branch is many, and blade is narrow.Particularly cockscomb to external world the resistance of adverse circumstance obviously reduce easy introduced disease.In order to improve the disease resistance of cultivating cockscomb and to view and admire characteristics, the present invention utilizes distant hybridization and backcrosses technology seed selection cockscomb new varieties (being).
Summary of the invention
The objective of the invention is by cultivation cockscomb and the distant hybridization of feather cockscomb and the cockscomb new varieties that backcross breeding goes out to have new breediness and disease resistance.
Technical scheme of the present invention is: a. selects edge parent far away and hybridizes and HORMONE TREATMENT; B. select hybrid and another cultivar to backcross; C. the hybrid generation selects; D. be stable into kind (being).
Detailed process of the present invention is:
A. select edge parent far away and hybridize and HORMONE TREATMENT,
To cultivate cockscomb (
Celosia cristataL.) make female parent, feather cockscomb (
Celosia argenteaL.) make male parent.Select the inflorescence of blooming 2～3 days their 6 o'clock～9 o'clock morning in season of flowers July～September, remove base portion open Xiao Hua and the too tender Xiao Hua in top with sharp tweezers, push again the Xiao Hua that will bloom the same day aside, choose except flower pesticide, castrate in succession 20～30 and put the hybridization paper bag after spending; By the time 10:30～12:00, when feather cockscomb has just been bloomed, take out uncracked flower pesticide in little culture dish with tweezers, accumulate to 30～40 when colored, glue feather cockscomb pollen with clean writing brush, be coated on the cockscomb column cap of having castrated.Every cockscomb flower on average is coated with the pollen amount of 4～5 on feather cockscomb flower, and then bagging is write hybrid strain and date.That afternoon, 4:30～6:00 used the in-built 30mg/l of laryngeal spray～50mg/l gibberellin (GA3)+10mg/l～20mg/l NAA(methyl α-naphthyl acetate) hormone liquid, facing to spraying 3～5 times in the hybridization bag, to drench column cap as standard; Continuous spraying 5 days;
B. select hybrid and another cultivar to backcross,
To determine that hybrid after the hybridization of cockscomb and feather cockscomb as female parent, selects the short strong cultivation cockscomb kind of another plant and makes male parent from the morphology, and backcross by " a " one step process, but need not spray hormone liquid;
C. the hybrid generation selects,
Hybrid generation after b processes, at plant height, on inflorescence types and the branch there be obvious the separation, according to breeding objective, select have many fringes, tower, torch-shaped, redness or yellow, the hybrid of the new proterties such as poly inflorescence, the many crown types of short life is selected and remain the offspring, carry out directive breeding, the inflorescence that per generation selects and remain kind will entangle with large hybridization bag, prevents the insect loose powder;
D. be stable into kind (being).
After 6～9 generations were selected and remain plantation, new proterties all tended to stable, and according to breeding objective, seed selection becomes stable new varieties (being), and is red such as thousand fringes of multi-spike-type, the Hongta woods of tower, yellow Tallin, short many hats of the many crown types of short life, flourishing torch etc.
The cockscomb new varieties (being) of institute of the present invention seed selection have following several effect:
1, the merit hybridization with the wild species feather cockscomb is attached in the new varieties, has formed the new varieties of multi-spike-type, and is red such as thousand fringes.
2, result from the new proterties that cockscomb combines with feather cockscomb, such as Hongta woods, yellow Tallin, flourishing torch, short many hats.
3, new varieties (being) not only have the new characteristics of viewing and admiring, and have stronger disease-resistant characteristics.
Embodiment
The present invention is further described with embodiment for the below
The embodiment red seed selection of fringe in 1: thousand
A. select edge parent far away and hybridization and HORMONE TREATMENT
Take redness cultivation cockscomb as maternal, take No. 1, feather cockscomb as male parent.Red cockscomb plant height 60cm～75cm, the crown inflorescence of single redness, red blade, pale red stem stalk; No. 1 plant height 138cm～170cm of feather cockscomb, multi-branched, panicled spike, the inflorescence tip is lilac red, leaf green, the stem stalk is green.
At 6:00～9:00 in the morning of late July to mid-September in Wuhan, red cockscomb is carried out flower thinning and castrates and process, and the cover paper bag; The flower pesticide that 10:30～12:00 gets No. 1, feather cockscomb with tweezers is coated on the column cap that red cockscomb castrated with the sticking pollen of clean writing brush in little culture dish, every pollen amount with No. 1,4～5 feather cockscomb, then bagging.That afternoon, 4:30～6:30 sprayed 3 times with the hormone liquid that laryngeal spray sprays GA3+10mg/l that 30mg/l～50mg/l is housed～20mg/l NAA, was advisable to drench column cap, processed continuously 5 days.
B. select hybrid and short yellow cockscomb to backcross
Pick out the hybrid of cockscomb and feather cockscomb from plant leaf size and branch proterties and inflorescence shape etc., the blade of hybrid is less and larger than feather cockscomb than cockscomb, and multi-branched, inflorescence become spike, and plant height reaches 134cm～152cm.Take the hybrid of picking as maternal, short yellow cockscomb is that male parent backcrosses, hybridization program such as a, but without HORMONE TREATMENT.
C. the hybrid generation selects
Hybrid generation after b processes at plant height, has obvious the separation on inflorescence types and the branch, wherein picks out red stem stalk, red blade, multi-branched, the directive breeding of spike flower type.
D. be stable into kind (being)
Behind continuous 6 generation self cross and selections, about a plant height 125cm, have red stem stalk, red blade, multi-branched, the cockscomb new lines (kind) of red spike flower has been stablized moulding, called after " thousand fringes are red ".
Embodiment 2: the seed selection of " Hongta woods "
Program a～b is identical such as the red seed selection of thousand fringes, and the proterties of selecting in the c process focuses on the characteristics of red multi-branched light-house shaped.Behind 9 generation self cross and selections, a plant height 90cm～110cm, multi-branched, red blade and stem stalk, every branch are gathered into the cockscomb new lines (kind) of tower inflorescence and have stablized moulding, called after " Hongta woods ".
Claims (1)
1. method of utilizing distant hybridization and backcrossing technology seed selection cockscomb kind is characterized in that Breeding Process is:
A. select edge parent far away and hybridize and HORMONE TREATMENT,
To cultivate cockscomb does maternal, feather cockscomb is made male parent, select the inflorescence of blooming 2～3 days their 6 o'clock～9 o'clock morning in season of flowers July～September, remove base portion open Xiao Hua and the too tender Xiao Hua in top with sharp tweezers, push again the Xiao Hua that will bloom the same day aside, choose except flower pesticide, castrate in succession 20～30 and put the hybridization paper bag after spending; By the time 10:30～12:00, when feather cockscomb has just been bloomed, take out uncracked flower pesticide in little culture dish with tweezers, accumulate to 30～40 when colored, glue feather cockscomb pollen with clean writing brush, be coated on the cockscomb column cap of having castrated; Every cockscomb flower on average is coated with the pollen amount of 4～5 on feather cockscomb flower, and then bagging is write hybrid strain and date; That afternoon, 4:30～6:00 used the in-built 30mg/l of laryngeal spray～50mg/l gibberellin+10mg/l～20mg/l NAA(methyl α-naphthyl acetate) hormone liquid, facing to spraying 3～5 times in the hybridization bag, to drench column cap as standard; Continuous spraying 5 days;
B. select hybrid and another cultivar to backcross,
To determine that hybrid after the hybridization of cockscomb and feather cockscomb as female parent, selects the short strong cultivation cockscomb kind of another plant and makes male parent from the morphology, and backcross by " a " one step process, but need not spray hormone liquid;
C. the hybrid generation selects,
Hybrid generation after b processes, at plant height, on inflorescence types and the branch there be obvious the separation, according to breeding objective, select have many fringes, tower or torch-shaped, redness or yellow, the hybrid of poly inflorescence, the new proterties of the many crown types of short life is selected and remain the offspring, carry out directive breeding, the inflorescence that per generation selects and remain kind will entangle with large hybridization bag, prevents the insect loose powder;
D. be stable into kind,
After 6～9 generations were selected and remain plantation, new proterties all tended to stable, and according to breeding objective, seed selection becomes stable new varieties.
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| CN103168676B (en) * | 2013-03-08 | 2014-11-26 | 湖北大学 | New Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method |
| CN104823834B (en) * | 2015-04-23 | 2018-01-26 | 湖北大学 | Utilize epigenetics principle and the method for distant hybridization seed selection cockscomb new varieties |
| CN105794628B (en) * | 2016-03-18 | 2017-11-17 | 湖北大学 | It is a kind of to utilize chimera cockscomb and the method for feather cockscomb selection cross feather cockscomb kind |
| CN112136682B (en) * | 2020-09-17 | 2021-05-04 | 云南农业大学 | Method for creating new germplasm by wild distant hybridization of rhododendron |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1395823A (en) * | 2002-08-09 | 2003-02-12 | 湖北大学 | Method for selectively breeding specific cockscomb by chromosome chimerism principle |
| CN1868258A (en) * | 2006-06-29 | 2006-11-29 | 北京锦绣大地农业股份有限公司 | Medium for culturing grown-up cockscombin sealed vessel, and culture method for induction blossom |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1395823A (en) * | 2002-08-09 | 2003-02-12 | 湖北大学 | Method for selectively breeding specific cockscomb by chromosome chimerism principle |
| CN1868258A (en) * | 2006-06-29 | 2006-11-29 | 北京锦绣大地农业股份有限公司 | Medium for culturing grown-up cockscombin sealed vessel, and culture method for induction blossom |
Non-Patent Citations (1)
| Title |
|---|
| 杜彦章.鸡冠花栽培技术.《现代农村科技》.2010,(第19期),第37页. * |
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Inventor after: Cai Detian Inventor after: Chen Dongling Inventor after: Song Zhaojian Inventor after: Gao Qingqing Inventor after: He Nisha Inventor after: Lu Zhenghui Inventor after: Xu Xiangjun Inventor before: Gao Qingqing Inventor before: He Nisha Inventor before: Lu Zhenghui Inventor before: Xu Xiangjun Inventor before: Chen Dongling Inventor before: Song Zhaojian Inventor before: Cai Detian |
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Free format text: CORRECT: INVENTOR; FROM: GAO QINGQING HE NISHA LU ZHENGHUI XU XIANGJUN CHEN DONGLING SONG ZHAOJIAN CAI DETIAN TO: CAI DETIAN CHEN DONGLING SONG ZHAOJIAN GAO QINGQING HE NISHA LU ZHENGHUI XU XIANGJUN |
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