CN101595838A - A kind of method of mango artificial hybridization pollination - Google Patents
A kind of method of mango artificial hybridization pollination Download PDFInfo
- Publication number
- CN101595838A CN101595838A CNA2009100407657A CN200910040765A CN101595838A CN 101595838 A CN101595838 A CN 101595838A CN A2009100407657 A CNA2009100407657 A CN A2009100407657A CN 200910040765 A CN200910040765 A CN 200910040765A CN 101595838 A CN101595838 A CN 101595838A
- Authority
- CN
- China
- Prior art keywords
- flower
- mango
- pollination
- bagging
- culture dish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of method of mango artificial hybridization pollination.It comprises C, gathers the mango paternal pollen; D, castration pollination, it is characterized in that: among the described step C, the fresh flower of adopting is positioned in the culture dish, after collection is finished, culture dish is placed on the sunlight direct projection or the local back up of incandescent lamp irradiation arranged and make culture dish be in opening-wide state, can carry out next step after the flower pesticide loose powder of the 5-10% that has an appointment in the male parent flower to be collected and castrate pollination; Among the described step D, castrate after pollination finishes, and bagging is squirted fully, and bagging is in kept moisture state 2-5 hour with sprayer.The present invention effectively utilizes the short period of mango flower loose powder to pollinate, and construction is beneficial to the super-humid conditions of pollen germination, thereby improved mango artificial pollination ripening rate greatly, the pollination ripening rate of mango can be stabilized in about 2% or higher according to flower number statistics, show that through test of many times this method is reliable and stable.
Description
Technical field
The present invention relates to the plant hybridization breeding technique, specifically be meant a kind of method of mango artificial hybridization pollination.
Background technology
Mango is as one of important fruit in the world, and annual production reaches 2796.67 ten thousand tons, and harvest area reaches 387.02 ten thousand hectares (FAO, 2005).The mango production development in recent years of China is swift and violent, 12.04 ten thousand hectares of national cultivated areas (Ministry of Agriculture's statistics does not comprise Taiwan Province, down together) in 2006, be the third-largest fruit that China's torrid areas output is only second to oranges and tangerines and banana, mango had become the pillar industry in China's hot-zone agricultural already.
China's mango industry exists the good new varieties shortage of independent intellectual property right, reserve kind deficiency, breed structure is unreasonable, the improved seeds coverage rate is low problem, present main breed is No. 6, No. 1, platform farming, Jin Huang, Kate, white elephant tooth, highest-ranking imperial concubine, osmanthus heat 82, red awns, these main breeds are except osmanthus heat 82 is the kind of China's autonomous innovation, other main breeds are overseas or external introduced variety, and there is bigger gap in country such as China's mango breeding level and mango breeding research advanced country India, Australia, Thailand, the U.S..Drawbacks such as the mango breeding has traditional grow directly from seeds breeding and artificial hybridization pollination breeding, and there is inefficiency in traditional breeding method of growing directly from seeds, and breeding objective is indeterminate; Conventional artificial hybridization breeding method exists hybridization technique immature, it is fewer worldwide to utilize at present the artificial hybridization pollination technique to carry out putting into practice of mango breeding, the mango kind in the world about 80% seed selection of growing directly from seeds at present, about in addition 20% kind also majority is that guard controlled pollination method and the seed selection of mixing spontaneous pollination method form, and artificial hybridization pollination improved variety is few, mango artificial hybridization pollination technique is a technical barrier in China, relevant report is few, the later stage seventies 20th century is to the beginning of the eighties, carry out mango artificial hybridization work in Guangxi, obtained 36 filial generation individual plants.Also rare report in the world wide, 1.2 ten thousand flowers of Fla. artificial hybridization are only received 47 of hybridization fruits.Mango artificial hybridization pollination mainly shows as following characteristics: the first, and inefficiency, the pollination ripening rate is low; The second, the labour cost height; The 3rd, the time cycle is long.Because the artificial hybridization ripening rate is low, be difficult to a certain amount of hybridization of acquisition colony, limited the research of mango artificial hybridization seed selection kind too, therefore solving the low problem of artificial hybridization pollination ripening rate is key point.
Summary of the invention
In order to overcome above-mentioned deficiency, the object of the present invention is to provide a kind of method that can effectively improve a kind of mango artificial hybridization pollination of mango artificial hybridization pollination ripening rate.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: the method for a kind of mango artificial hybridization pollination may further comprise the steps: A, choose maternal inflorescence of mango and flower; B, the maternal flower of mango is isolated with bagging; C, collection mango paternal pollen; D, castration pollination; E, removing the bagging of pollination flower isolates, it is characterized in that: among the described step C, the 3rd day after the maternal flower of mango is isolated with bagging early in the morning, gather fresh flower just in full bloom on the male parent tree and comprise unisexuality male flower and hermaphrodite flower, the fresh flower of adopting is positioned in the culture dish, after collection is finished, culture dish is placed on the sunlight direct projection or the local back up of incandescent lamp irradiation arranged and make culture dish be in opening-wide state, treat that anther color is transferred to by redness or part transfers canescence to, show that flower pesticide has begun loose powder, can carry out next step after the flower pesticide loose powder of the 5-10% that has an appointment in the male parent flower to be collected and castrate pollination; Among the described step D, after the castration pollination is finished, the bagging of untiing is put once more, and bagging is squirted fully, and make bagging be in maintenance moisture state 2-5 hour with sprayer.
Bagging among the described step B adopts the good sheepskin paper bag of water-permeable and air permeable light transmission.
Among the described step D, when castrating pollination, untie the bagging of the inflorescence of will pollinating, pluck unisexuality male flower, teleianthous stamen in full bloom and do not have flower in full bloom with tweezers earlier, directly flower pesticide is applied on the column cap of hermaphrodite flower gynoecium with tweezers then and gets final product, the pollen of 1 flower is smeared 5 flowers at most, and it is light that strength is wanted, can not damage gynoecium, the column cap that affirmation pollen is run into gynoecium gets final product.
In the described step e, the time of separating bagging is the 4th day after pollinating.
Beneficial effect of the present invention is: according to mango flowering habit feature, the present invention effectively utilizes the short period of mango flower loose powder to pollinate, and construction is beneficial to the super-humid conditions of pollen germination, thereby improved mango artificial pollination ripening rate greatly, whole pollinating process is simple, the pollination ripening rate of mango can be stabilized in about 2% or higher according to flower number statistics, show through test of many times, this method is reliable and stable, provides ripening rate assurance comparatively reliably for scientific and technical personnel carry out mango artificial hybridization pollination.
Embodiment
A kind of method of mango artificial hybridization pollination may further comprise the steps:
A, choose maternal inflorescence of mango and flower: have the healthy and strong inflorescence of a small amount of blossom to choose flower in the middle part of choosing tree body periphery in bloom early stage and the Sheng phase of stock tree, the flower (about 20-35) of choosing in the inflorescence middle and upper part the 2nd day then or will blooming in the 3rd day, Sheng Kai flower (can see colored gynoecium and column cap) and too young tender bud are removed with tweezers;
B, the maternal flower of mango is isolated with bagging: with the water-permeable and air permeable light transmission preferably the sheepskin paper bag (specification is: 40cm * 34cm, also can have made to order as required, can buy to this type of enterprise of Taizhou, Zhejiang) put inflorescence and chosen the part of the flower of pollinating and (will choose comparatively strong bagging, can reduce of the damage of bagging process greatly like this to gynoecium, should not choose the bagging of soft easy damaged gynoecium), the main purpose of bagging is to prevent insect pollination such as fly;
C, gather the mango paternal pollen: the 3rd day (midfeather 1 day) after choosing pollination inflorescence and flower is gathered fresh flower just in full bloom on the male parent tree (can see that anther color is red) early in the morning and is positioned in the culture dish, the fresh flower of gathering comprises that the unisexuality male flower (has only stamen, do not have gynoecium) and hermaphrodite flower (stamen is arranged promptly, gynoecium is arranged) again, the local back up that after having gathered culture dish is placed on the sunlight direct projection (promotes the flower pesticide loose powder of splitting as early as possible, there is not the weather condition available incandescent lamp irradiation of sunlight), treat that anther color is transferred to by redness or part transfers canescence to, just flower pesticide just splits, the color that the flower pesticide very first time changes, show that flower pesticide has begun loose powder, can carry out next step after the flower pesticide loose powder of 5-10% of having an appointment in the flower to be collected and castrate pollination, because castrate pollination on one side, the flower pesticide loose powder of can splitting one by one of residue flower, do not influence the pollination process of castrating, depositing flower gets final product with culture dish, do not cover, otherwise split can be because culture dish the inside high humidity after the loose powder for the flower pesticide of flower in the culture dish, the flower pesticide that splits can become black very soon, pollen clumps together, and is unfavorable for pollination;
D, castrate pollination: the bagging of untiing the inflorescence of to pollinate, pluck unisexuality male flower in full bloom with tweezers earlier, pollinate teleianthous stamen (castration) and do not have flower (being as the criterion) in full bloom with the pistil stigma that can not see flower, at last the hermaphrodite flower gynoecium is pollinated, after the flower pesticide loose powder to be collected (concrete criterion saw for the 2nd step), directly flower pesticide is applied in and gets final product on the column cap of the flower of pollinating that (1 flower pesticide is smeared 5 flowers at most with tweezers, unsuitable too many), it is light that strength is wanted, can not damage gynoecium, confirm that pollen suffers (bumping) and gets final product to the column cap of gynoecium, finish the pollination work of each flower after the same method, then the bagging of untiing is put once more (noting not being damaged to as far as possible gynoecium this moment), bagging is squirted fully (high humidity helps being fertilized solid) with sprayer after putting bagging, carrying out every record with label then hangs on the pollination inflorescence, with sprayer the bagging that packages is squirted at regular intervals afterwards, keep bagging to be in moisture state 2-5 hour (pollen is finished germination process and was generally 2-5 hour always, this moment, high humidity was beneficial to pollen germination, was beneficial to gynoecium and maintained vigour).
E, the bagging of removing the pollination flower are isolated: separate bagging after pollination the 3rd day (midfeather 2 days) and directly untie bagging and get final product, strength is lighter, avoids damage pollination flower, notes checking do not remove clean flower not in full bloom when whether pollination is arranged this moment, if have, remove clean.Carry out mark (assurance has allocarpy to be convenient in real time seek), as pricking on the pollination inflorescence with red cloth.
Embodiment
Example 1: carry out mango artificial hybridization test of pollination in mango proving ground, cane breeding field, Sanya, Hainan Guangzhou Inst of Cane Sugar Hainan in the 11-12 month in 2007, with the bright kind of gold is female parent, like that civilian kind is that male parent is hybridized, 679 inflorescences of pollinating altogether, 10066 flowers are received 219 of hybridization fruits in April, 2008, obtain 184 strains of hybridization seedling in July, 2008, ripening rate is 2.18% by the flower statistics, uses the SSR molecular labeling hybridization seedling is identified that 184 strains are true hybrids.
Example 2: carry out mango artificial hybridization test of pollination in mango proving ground, cane breeding field, Sanya, Hainan Guangzhou Inst of Cane Sugar Hainan in early January, 2009, with the bright kind of gold is female parent, the Kensington kind is that male parent is hybridized, 60 inflorescences of pollinating altogether, 454 flowers, receive 15 of hybridization fruits on April 28th, 2009, obtain 15 strains of hybridization seedling in May, 2008, ripening rate is 3.30% by the flower statistics.
Example 3: carry out mango artificial hybridization test of pollination in mango proving ground, cane breeding field, Sanya, Hainan Guangzhou Inst of Cane Sugar Hainan in early January, 2009, with the bright kind of gold is female parent, No. a kind of heat farming is that male parent is hybridized, 62 inflorescences of pollinating altogether, 549 flowers, receive 12 of hybridization fruits on April 28th, 2009, obtain 12 strains of hybridization seedling in May, 2008, ripening rate is 2.19% by the flower statistics.
Claims (4)
1, the method for a kind of mango artificial hybridization pollination may further comprise the steps: A, choose maternal inflorescence of mango and flower; B, the maternal flower of mango is isolated with bagging; C, collection mango paternal pollen; D, castration pollination; E, removing the bagging of pollination flower isolates, it is characterized in that: among the described step C, the 3rd day after the maternal flower of mango is isolated with bagging early in the morning, gather fresh flower just in full bloom on the male parent tree and comprise unisexuality male flower and hermaphrodite flower, the fresh flower of adopting is positioned in the culture dish, after collection is finished, culture dish is placed on the sunlight direct projection or the local back up of incandescent lamp irradiation arranged and make culture dish be in opening-wide state, treat that anther color is transferred to by redness or part transfers canescence to, show that flower pesticide has begun loose powder, can carry out next step after the flower pesticide loose powder of the 5-10% that has an appointment in the male parent flower to be collected and castrate pollination; Among the described step D, after the castration pollination is finished, the bagging of untiing is put once more, and bagging is squirted fully, and make bagging be in maintenance moisture state 2-5 hour with sprayer.
2, the method for mango artificial hybridization pollination according to claim 1, it is characterized in that: the bagging among the described step B adopts the good sheepskin paper bag of water-permeable and air permeable light transmission.
3, the method for mango artificial hybridization pollination according to claim 1, it is characterized in that: when castrating pollination, untie the bagging of the inflorescence of will pollinating, pluck unisexuality male flower, teleianthous stamen in full bloom and do not have flower in full bloom with tweezers, directly flower pesticide is applied on the column cap of hermaphrodite flower gynoecium with tweezers then and gets final product, the pollen of 1 flower is smeared 5 flowers at most, and it is light that strength is wanted, can not damage gynoecium, the column cap that affirmation pollen is run into gynoecium gets final product.
4, the method for mango artificial hybridization pollination according to claim 1 is characterized in that: in the described step e, the time of separating bagging is the 4th day after pollinating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100407657A CN101595838A (en) | 2009-07-01 | 2009-07-01 | A kind of method of mango artificial hybridization pollination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100407657A CN101595838A (en) | 2009-07-01 | 2009-07-01 | A kind of method of mango artificial hybridization pollination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101595838A true CN101595838A (en) | 2009-12-09 |
Family
ID=41417579
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2009100407657A Pending CN101595838A (en) | 2009-07-01 | 2009-07-01 | A kind of method of mango artificial hybridization pollination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101595838A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599049A (en) * | 2012-03-28 | 2012-07-25 | 何寒 | Pollination method of mango by breeding flies in flowering phase for propagation |
| CN103348911A (en) * | 2013-07-04 | 2013-10-16 | 中国热带农业科学院热带作物品种资源研究所 | Artificial mango crossbreeding method |
| CN111296284A (en) * | 2020-04-08 | 2020-06-19 | 西北农林科技大学 | Efficient walnut crossbreeding method |
-
2009
- 2009-07-01 CN CNA2009100407657A patent/CN101595838A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599049A (en) * | 2012-03-28 | 2012-07-25 | 何寒 | Pollination method of mango by breeding flies in flowering phase for propagation |
| CN103348911A (en) * | 2013-07-04 | 2013-10-16 | 中国热带农业科学院热带作物品种资源研究所 | Artificial mango crossbreeding method |
| CN111296284A (en) * | 2020-04-08 | 2020-06-19 | 西北农林科技大学 | Efficient walnut crossbreeding method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Corley | Potential productivity of tropical perennial crops | |
| CN101816282A (en) | Hybrid round eggplant seed production method | |
| CN104770264A (en) | Tea tree cultivation method for increasing yield of tea seeds | |
| CN102334445A (en) | Pollination method for purity-conservation Chinese cabbage breeding material | |
| CN110073968A (en) | A kind of fast breeding method of high oily salt tolerant peanut | |
| CN103348911A (en) | Artificial mango crossbreeding method | |
| CN103828708A (en) | Efficient soybean cytoplasmic male sterility test-crossing method and device | |
| Akoroda | Floral biology in relation to hand pollination of white yam | |
| CN107432243A (en) | A kind of selection of cold-resistant, resistance to bolting cabbage | |
| CN104303731A (en) | Zucchini breed hybrid seed production method | |
| CN101595838A (en) | A kind of method of mango artificial hybridization pollination | |
| CN103798130A (en) | Watermelon hybrid seed production method | |
| CN106973784A (en) | A kind of breeding method of New Strawberry Variety | |
| CN102668975A (en) | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker | |
| Kishore | Phenological growth stages of Indian gooseberry (Phyllanthus emblica L.) according to the extended BBCH scale | |
| CN101861828B (en) | Pineapple hybridization method | |
| CN104957028A (en) | High-yield hybrid seed production method of cotton | |
| CN108739364B (en) | Method for improving seed production efficiency of self-incompatible gramineous plant hybridization | |
| CN109349016B (en) | Method for rapidly propagating chicory seeds in Nanjing area | |
| CN107155873A (en) | One kind makes the acarpous producing method for seed of hybrid paddy rice male parent | |
| CN105075852A (en) | Method for hybridizing and breeding water dropwort | |
| CN101116419A (en) | Centipede grass cross-breeding method | |
| Pitsioudis et al. | Early and late production of raspberries, blackberries and red currants | |
| CN102823386A (en) | Efficient recovery method of stevia seeds | |
| CN108575595A (en) | Hybrid Chinese pennisetum high yield cultivating method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20091209 |