CN106688876A - Combining and matching method of maize - Google Patents
Combining and matching method of maize Download PDFInfo
- Publication number
- CN106688876A CN106688876A CN201710021651.2A CN201710021651A CN106688876A CN 106688876 A CN106688876 A CN 106688876A CN 201710021651 A CN201710021651 A CN 201710021651A CN 106688876 A CN106688876 A CN 106688876A
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- Prior art keywords
- pollination
- maternal
- row
- maize
- female
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
- A01H1/027—Apparatus for pollination
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a combining and matching method of maize. A plurality of kinds of maize female parent rows are planted in a direction perpendicular to a male parent row; before pollination, female ears of female parents are covered by using white parchment paper bags to be isolated; during the pollination, pollen is intensively collected from male parents and is sub-packaged in red parchment paper bags; the pollinated female ears of the female parents are isolated and marked by using the red parchment paper bags; during the pollination, boards are hung on female parent plants to mark combination numbers. The combining and matching method has the beneficial effects that the plantation quantity of the male parents is greatly reduced; the hybridization is convenient; the marking is convenient; the workload is decreased.
Description
Technical field
The invention belongs to corn hybridization breeding field, and in particular to a kind of Maize Combination compound formulation.
Background technology
Corn is China cultivated area maximum cereal crops, and Maize Production has very important war to China's grain security
Slightly use, corn seed is the basis for ensureing Maize Production.The corn variety of current large-scale promotion is all the seed of single cross, the seed of single cross
What is utilized is the yield and general performance that male parent improves the first generation of hybrid with the hybrid vigour of female parent.But because current hybrid is excellent
The study mechanism level of gesture lags far behind application level, and Heterosis of Maize Hybrid is using the field for relying primarily on male parent group and maternal group
Between be measured and determine the coordinate force of parents, and then determine whether parents can be used to assemble new varieties, so corn breeding works
In a large amount of parental combinations assemble be work emphasis be also work difficult point.Maize Combination testing experiment is generally divided into and is measured examination
Test, preliminary identification, middle rank identify and senior identification that experiments at different levels are screened layer by layer.Into senior qualification test combination due to
Kind of amount typically carries out the production of hybrid seeds using isolated area greatly, and being measured test combinations only needs to be measured parent and do 1-2 fringes, workload with several
Also it is little；Preliminary identification and intermediate qualification test combination are the emphasis of work, and general corn breeding team needs to assemble every year
Thousands of or up to ten thousand combinations, preliminary identification tests general sowing quantity to be needed to do 3-5 fringes, and middle rank experiment needs 10-20 fringes, so many
Corn ear according to traditional breeding method（Plantation line number is determined according to number of combinations when each female parent material is planted, it is different
Combination marked using each individual plant after different male parent artificial pollinations, listed workload is big and easily occurs obscuring and waits mistake
By mistake；If the female parent of various combination is planted different head progeny rows and the excessive problem of land used occurs again respectively）, each is required for investigating father
The fields such as maternal florescence, artificial pollination, listing mark operate, and workload is big and is easy to mistake occur.
The present invention improves existing Maize Combination parent cultivation and labeling method, overcomes workload and is asked with error-prone greatly
A kind of topic, it is proposed that Maize Combination compound formulation.
The content of the invention
It is an object of the present invention to provide a kind of Maize Combination compound formulation, parent's plantation amount is reduced, male parent female parent position connects
It is accessible to be easy to mark in pollination, it is difficult to obscure.
The present invention is achieved through the following technical solutions：
The present invention provides a kind of Maize Combination compound formulation, is implemented by following steps：
（1）Plantation a line or some row corn male parent rows；
（2）Several corn female parent row, every kind of corn planting of maternal a line or some rows are planted in the direction vertical with male parent row；
（3）Maternal female fringe is covered before pollination with white sulfuric acid paper bag to be isolated；
（4）During pollination, concentrated after 11 points of every morning to male parent powder collecting, noon is in shady and cool ground or interior according to pollination demand to father
This pollen is dispensed, and is dispensed into red sulfuric acid paper bag；
（5）That afternoon is pollinated, and the maternal female fringe of pollination substitutes white sulfuric acid paper bag and isolated with red sulfuric acid paper bag
And mark；
（6）In pollination, the listing mark combination number on maternal plant.
Preferably, step（1）Male parent row listing mark title.
Preferably, step（2）Maternal row listing mark title.
Preferably, step（6）Combination labelled notation is only hung on first female parent for close on male parent.
It is furthermore preferred that on male parent row two or the plant of listing mark title.
It is furthermore preferred that on maternal row two or the plant of listing mark title.
The beneficial effects of the invention are as follows：
1st, the plantation amount of male parent is greatly reduced.Traditional corn assembles the male parent and planting of maternal that implantation methods are tissue cultures and is putting down
Capable adjacent lines, in order to hybridization pollination.So need to plant substantial amounts of male parent, paternal pollen to pollination is taken when otherwise pollinating
It is maternal to be pollinated inconvenient away from too far away, increased workload.The present invention only needs to plant a line or some row male parents, can be for
Many maternal pollinations are used.
2nd, convenient hybridization.Arrangement mode of the invention, makes each female parent have the chance closed on male parent row, during pollination
Collection pollen and the distance for completing to pollinate are short, are easy to hybridize and keep the activity of pollen.
3rd, it is convenient to mark.Centered on male parent, male parent row planted on two sides is maternal, is easy to distinguish male parent and female parent, while female
This is marked with male parent behavior and gone, and is not listed and also can distinguish different female parents according to plantation record.
4th, workload reduction.Male parent plantation amount is reduced, and artificial pollination distance is near, and mark is convenient, or even each composite marking
One just, and these designs greatly reduce workload.
5th, carried out between Bian powder and pollination timesharing, work is transferred to interior by most hot period at noon, prevent personnel's fatigue, significantly
Improve pollination efficiency.
6th, white sulfuric acid paper bag is substituted using red sulfuric acid paper bag during Bian powder, during pollination, is easy to mark the plant pollinated
Strain.
Specific embodiment：
It is used for a kind of Maize Combination compound formulation the invention discloses one kind, those skilled in the art can use for reference present disclosure,
It is suitably modified parameter realization.In particular, all similar replacements and change be for a person skilled in the art
It will be apparent that they are considered as being included in the present invention.Preferred embodiment disclosed by the invention, those skilled in the art can be
Not to be departed from present invention, spirit and scope and method described herein and application are modified or is suitably changed and is combined,
To realize and apply the technology of the present invention.By the following example by the more specific description present invention, it should be understood that the implementation
Example merely to the explanation present invention, rather than limiting the scope of the present invention by any way.
The farm Maize Combination of embodiment 1 six one assembles experiment
Maize Combination is carried out on 61 farms within 2015 assemble experiment.Select selection isolation condition preferable plot and breeding is set
Field.The row of lx03-2 male parents 3 is planted in breeding Tanaka centre, male parent row is long 50 meters, line-spacing 40cm, in the listed mark in 3 plants of male parent row two
Note male parent.Male parent row both sides and male parent vertical direction plant maternal, and the row of every kind of planting of maternal 1, row is long 5 meters, line-spacing 40cm, with
The neighbouring plant of male parent row is listed 3.Maternal female fringe spinning protheca white sulfuric acid paper bag isolation.Male parent Post flowering needs pollination
When, loose powder is vigorous after 11 points of that morning and collection pollen pollination when air humidity reduction, is p.prand carried out in workplace
Pollen is dispensed, and the pollen after packing is placed in the cool, and that afternoon is pollinated after resting, and white is extractd during pollination
Sulfuric acid paper bag, with red sulfuric acid paper bag bagging.The maternal row pollinated, writes with 3 plant of male parent row one end farther out are listed
The maternal combination of upper male parent.During results, cenospecies is packed, the swatch of mark combination when sack fastens hybridization.If forgotten during pollination
Note writes combination swatch, can register combination according to the maternal notice plate of neighbouring male parent.
Compared with the maternal parallel adjacent seed of former years male parent, above-mentioned planting patterns is taken to greatly reduce the kind of male parent
Plant, all maternal and male parents are neighbouring, pollination is convenient timely, save that pollination is artificial, while be transferred to for work by most hot period at noon
Interior, prevents personnel's fatigue, substantially increases pollination efficiency.The mark mode of this experiment also easily facilitates sign with traditional approach
Hybridization and non-hybridized plant, only listing mark female parent title can also distinguish combination to maternal plant.
Claims (6)
1. a kind of Maize Combination compound formulation, is implemented by following steps：
（1）Plantation a line or some row corn male parent rows；
（2）Several corn female parent row, every kind of corn planting of maternal a line or some rows are planted in the direction vertical with male parent row；
（3）Maternal female fringe is covered before pollination with white sulfuric acid paper bag to be isolated；
（4）During pollination, concentrated after 11 points of every morning to male parent powder collecting, noon is in shady and cool ground or interior according to pollination demand to father
This pollen is dispensed, and is dispensed into red sulfuric acid paper bag, and the pollen after packing is placed into shady place；
（5）That afternoon is pollinated, and the maternal female fringe of pollination substitutes white sulfuric acid paper bag and isolated with red sulfuric acid paper bag
And mark；
（6）In pollination, the listing mark combination number on maternal plant.
2. a kind of Maize Combination compound formulation according to claim 1, it is characterised in that：Step（1）The listed mark of male parent row
Sign title.
3. a kind of Maize Combination compound formulation according to claim 1, it is characterised in that：Step（2）The listed mark of maternal row
Sign title.
4. a kind of Maize Combination compound formulation according to claim 1, it is characterised in that：Step（6）Only closing on father
Combination labelled notation is hung on this first female parent.
5. a kind of Maize Combination compound formulation according to claim 2, it is characterised in that：In male parent row two or one
Listing mark title on plant.
6. a kind of Maize Combination compound formulation according to claim 3, it is characterised in that：In maternal row two or one
Listing mark title on plant.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710021651.2A CN106688876A (en) | 2017-01-12 | 2017-01-12 | Combining and matching method of maize |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710021651.2A CN106688876A (en) | 2017-01-12 | 2017-01-12 | Combining and matching method of maize |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106688876A true CN106688876A (en) | 2017-05-24 |
Family
ID=58908348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710021651.2A Pending CN106688876A (en) | 2017-01-12 | 2017-01-12 | Combining and matching method of maize |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106688876A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113475331A (en) * | 2021-08-05 | 2021-10-08 | 张掖市易农农业科技有限公司 | Planting method for corn DH line combining ability identification |
| CN116058277A (en) * | 2022-09-08 | 2023-05-05 | 广西南亚热带农业科学研究所 | Tea tree hybridization marking method and early seedling breeding method of hybrid seeds |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334447A (en) * | 2010-07-23 | 2012-02-01 | 沈天民 | Cultivation method of maize inbred line |
| CN103999769A (en) * | 2014-06-06 | 2014-08-27 | 山东省农业科学院玉米研究所 | Corn haploid inducing method |
| CN105265261A (en) * | 2015-10-16 | 2016-01-27 | 苏州市农业科学院 | Test cross parent field arranging and transplanting method |
-
2017
- 2017-01-12 CN CN201710021651.2A patent/CN106688876A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102334447A (en) * | 2010-07-23 | 2012-02-01 | 沈天民 | Cultivation method of maize inbred line |
| CN103999769A (en) * | 2014-06-06 | 2014-08-27 | 山东省农业科学院玉米研究所 | Corn haploid inducing method |
| CN105265261A (en) * | 2015-10-16 | 2016-01-27 | 苏州市农业科学院 | Test cross parent field arranging and transplanting method |
Non-Patent Citations (1)
| Title |
|---|
| 秦家友等: "玉米自交高效套袋授粉技术", 《中国种业》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113475331A (en) * | 2021-08-05 | 2021-10-08 | 张掖市易农农业科技有限公司 | Planting method for corn DH line combining ability identification |
| CN116058277A (en) * | 2022-09-08 | 2023-05-05 | 广西南亚热带农业科学研究所 | Tea tree hybridization marking method and early seedling breeding method of hybrid seeds |
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Application publication date: 20170524 |