CN110235777A - A kind of field planting method efficiently assembling the combination of corn diallel cross - Google Patents
A kind of field planting method efficiently assembling the combination of corn diallel cross Download PDFInfo
- Publication number
- CN110235777A CN110235777A CN201910690444.5A CN201910690444A CN110235777A CN 110235777 A CN110235777 A CN 110235777A CN 201910690444 A CN201910690444 A CN 201910690444A CN 110235777 A CN110235777 A CN 110235777A
- Authority
- CN
- China
- Prior art keywords
- self
- row
- mating system
- male parent
- maternal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of field planting methods for efficiently assembling the combination of corn diallel cross, by male parent by row plantation, the same male parent self-mating system of same behavior；Female parent is by column plantation, it is same to be classified as same maternal self-mating system, the line number of each maternal column is equal to the species number of male parent self-mating system, the first trip plantation of the first row male parent self-mating system and all maternal column self-mating systems is in a row, second row male parent self-mating system and all maternal column the second row of self-mating system plantations are in a row, and so on, until the most footline plantation of most footline male parent self-mating system and all maternal column self-mating systems is in a row.When pollination, maternal self-mating system emasculation, male parent self-mating system divides Leie time harvest by the corresponding maternal column corresponding line self-mating system of pollinating of row in lines after mature.Present invention reduces the workloads and fussy degree of previous diallel cross formulated in combination link, reduce the probability that pollen mixes, and improve pollination and clear kind of efficiency, reduce fault caused by human negligence, are suitable for corn breeding in a wide range of and promote and apply.
Description
Technical field
The present invention relates to crossbreeding technology fields, in particular to a kind of field for efficiently assembling the combination of corn diallel cross
Between implantation methods.
Background technique
Hybrid vigour is heterozygote the phenomenon that being better than its two parents in one or more characters.Heterosis utilization
It is the basic ideas of corn breeding, approach is the parental inbred line of first breeding homozygosis, then parental inbred line is hybridized, and selects
Strong superior hybrid crosses.Therefore, corn breeding " difficult is to focus on and be measured in choosing ".Coordinate force refers to the group of each character between different parents
Conjunction ability, it be judge corn inbred line important indicator, be related to a self-mating system in cenospecies assembles purposes certainly
Qualitative factor.
Nineteen forty-two, sprague and taturn propose the concept of general combining ability and specific combining ability.General combining ability
(GCA) refer to a series of mean apparent of yield or other character of the parent in cross combinations；Specific combining ability (SCA)
Refer to the trait expression offset of specific combination and the difference of the predicted value obtained according to parents' general combining ability.It can be seen that GCA and
SCA must can just be determined come valuation each depends on a set of specific self-mating system for participating in hybridization by hybridization.
Sprague and taturm uses the GCA and SCA of diallel cross design analysis corn inbred line, griffing to set this first
It has been counted as comprehensive arrangement and research, has been allowed to more systematic and perfect.Diallel cross is divided into complete diallel crosses and endless
All diallel crossing design, total fertilization failure carry out all possible hybridization and selfing between referring to one group of P parent, not exclusively double
Column hybridization is will to be divided into two groups by test requirements document for examination parent, only carries out possible hybridization between group, the parent in same group does not do
Hybridization.Such as Fig. 1: p1=q1、p2=q2、p3=q3、p4=q4、p5=q5、…、pn=qn, then it is total fertilization failure；p1、p2、p3、p4、
p5、…、pn、q1、q2、q3、q4、q5、…、qnFor different self-mating systems, wherein p1、p2、p3、p4、p5、…、pnIt is maternal group, q1、q2、
q3、q4、q5、…、qnIt is then incomplete diallel cross for male parent group.Therefore, complete a set of diallel cross is obtained to be combined into
Implement the key of diallel cross test.
However, traditional assembles link (Fig. 2), usually will successively be planted for examination self-mating system by plant, each self-mating system is pressed
Test needs to plant a line or multirow, tens plants of every row.It, will be specific according to diallel cross plan when carrying out field pollination work
Self-mating system pollen collection is marked into sulfuric acid paper bag, then on the filigree of artificial pollination to corresponding self-mating system.Such as: being derived from
Friendship is that 1 pollen awards self-mating system 2, is marked；It takes the pollen of self-mating system 2 to award self-mating system 3, marks；Take the flower of self-mating system 3
Powder awards self-mating system 4, marks；And so on.A hybridization is often done in this way, is required round-trip field and is taken powder, pollination, checks miscellaneous
Plan is handed over, marks the combination hybridized and fruit ear quantity etc., intricate operation is slightly neglected, and is easy to malfunction；In the process of pollination
In, such as misoperation, then pollen is easy to go here and there powder and mixes.When harvest, one fruit ear of every receipts will check being listed on cornstalk,
Low efficiency, it is easier to malfunction, to influence the accuracy of subsequent Hybridization identification work.After harvest, it is also necessary to clear kind of sequence.With
On can reduce breeding efficiency, cause the serious waste of human, financial, and material resources power.
How to overcome corn diallel cross formulated in combination low efficiency, the limitation of intricate operation, explore and make it is a set of both
Simple and practical and efficient quality guaranteeing the field planting method for assembling diallel cross combination is of great significance.
Summary of the invention
For the deficiency of above-mentioned existing design method, corn biserial is efficiently assembled the object of the present invention is to provide a kind of
The field planting method of cross combination, solves that corn diallel cross test combinations confecting efficiency is low, cumbersome, to be easy to pollen mixed
The listed sowing link in miscellaneous and field is easy to the problems such as malfunctioning.
In order to solve the above-mentioned technical problem, present invention employs the following technical solutions: a kind of efficiently to assemble corn biserial
The field planting method of cross combination, comprising the following steps:
1) according to diallel cross test plan, male parent group self-mating system and maternal group self-mating system are determined；
2) male parent group self-mating system is taken, by male parent by row plantation, plants same male parent self-mating system, the line number etc. of male parent row with a line
In the species number of male parent self-mating system；
3) maternal group self-mating system is taken, by female parent by column plantation, the same maternal self-mating system of same row plantation, the columns etc. of female parent column
In the species number of maternal group self-mating system, line number is equal to the species number of male parent group self-mating system；And make the first row male parent self-mating system
In a row, the second row male parent and all maternal column the second row of self-mating system plantations are at one for first trip plantation with all maternal column self-mating systems
Row, and so on, until the most footline plantation of most footline male parent and all maternal column self-mating systems is in a row；
4) bagging before all self-mating system female fringes being spun, maternal self-mating system whole emasculation, bagging when male parent self-mating system tassel is bloomed
Powder is taken, by the corresponding pollination of every row male parent self-mating system to maternal column corresponding line self-mating system, i.e. the 1st row male parent all female parents of pollinating arrange the
1 row is maternal, and all female parents of the 2nd row male parent pollination arrange the 2nd row female parent, and all female parents of the 3rd row male parent pollination arrange the 3rd row female parent, with
This analogizes, and completes all diallel cross test combinations until preparing；
5) after pollinating, listing mark cross combination, after mature, branch point column harvest arranges the 1st row according to the 1st female parent,
2nd female parent arranges the 1st row, until n-th female parent arranges the 1st row, then the 1st female parent arranges the 2nd row, and the 2nd female parent arranges the 2nd row,
Until n-th female parent arranges the 2nd row, and so on arrive n-th female parent column Nth row.Branch point column harvest simplifies subsequent hybridization group
Close clear kind of work of sequence.
Further, the row of each male parent row is long determines according to the maternal quantity of diallel cross test；Preferably, with 7 meters
16 nests are planted in the long double strains of row.
Further, the male parent group self-mating system was divided to for two phases sowed, and the first phase and the maternal self-mating system same period, the second phase is than maternal
Self-mating system late sowing 3 days.
Further, cross combination sowing quantity needed for the long test according to diallel cross of row of each maternal column determines；It is excellent
Choosing, 4 nests are planted with the long double strains of 1.7 meters of rows.
Compared with prior art, the invention has the following beneficial effects:
1, the present invention plants same male parent self-mating system with a line by male parent by row plantation；Female parent is by column plantation, same row plantation
The line number of same female parent self-mating system, each maternal column is equal to the species number of male parent self-mating system, the first row male parent self-mating system and institute
Have the first trip plantation of maternal column self-mating system in a row, the second row male parent self-mating system and all maternal column the second row of self-mating system plantations at
A line, and so on, until the most footline plantation of most footline male parent self-mating system and all maternal column self-mating systems is in a row.Pollination
When, maternal self-mating system emasculation, by the corresponding maternal column corresponding line self-mating system of pollinating of every row male parent self-mating system, without being checked in pollination
Diallel cross plan, unified listing mark cross combination after pollination are mature without the listing mark combination title in pollination
Afterwards according to cenospecies sowing sequence branch point column harvest seed, without clearing up built-up sequence after sowing, present invention reduces biserials
The workload and fussy degree of link are prepared in cross combination, reduce the probability that pollen mixes, and improve pollination and clear kind of efficiency,
Reduce fault caused by human negligence.
2, it prepares diallel cross combination using previous method to need to arrange out Parent two to table, often doing a combination needs
It to be marked on table, in order to avoid repeat.The pollen for needing to hold male parent finds corresponding female parent in field to the sequence of table according to two,
Has quite skilled corn hybridization pollination technique therefore, it is necessary to operational staff.And it is double to use planting patterns of the invention to prepare
Column cross combination, it is not easy to make mistakes due to easy to operate, to the of less demanding of operational staff, to master corn pollination technology,
It can guarantee both quality and quantity to complete to assemble task, be conducive to realize normalizing operation, be particularly suitable in corn breeding R&D institution and company
It promotes and applies.
Detailed description of the invention
Fig. 1 is diallel cross formulated in combination schematic diagram；
Fig. 2 is the field planting schematic diagram that traditional group matches diallel cross combination；
Fig. 3 is the field planting schematic diagram for efficiently assembling diallel cross combination of the invention；
Fig. 4 is that the diallel cross that assembles of embodiment combines field planting schematic diagram.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail.
A kind of field planting method efficiently assembling the combination of corn diallel cross, comprising the following steps:
1) according to diallel cross test plan, male parent group self-mating system and maternal group self-mating system are determined；
2) male parent group self-mating system is taken, by male parent by row plantation, plants same male parent self-mating system, the line number etc. of male parent row with a line
In the species number of male parent self-mating system；
3) maternal group self-mating system is taken, by female parent by column plantation, the same maternal self-mating system of same row plantation, the columns etc. of female parent column
In the species number of maternal group self-mating system, line number is equal to the species number of male parent group self-mating system；And make the first row male parent self-mating system
In a row, the second row male parent and all maternal column the second row of self-mating system plantations are at one for first trip plantation with all maternal column self-mating systems
Row, and so on, until the most footline plantation of most footline male parent and all maternal column self-mating systems is in a row, specifically as shown in Figure 3；
4) bagging before all self-mating system female fringes being spun, maternal self-mating system whole emasculation, bagging when male parent self-mating system tassel is bloomed
Powder is taken, by the corresponding pollination of every row male parent self-mating system to maternal column corresponding line self-mating system, i.e. the 1st row male parent all female parents of pollinating arrange the
1 row is maternal, and all female parents of the 2nd row male parent pollination arrange the 2nd row female parent, and all female parents of the 3rd row male parent pollination arrange the 3rd row female parent, with
This analogizes, and completes all diallel cross test combinations until preparing；
5) after pollinating, listing mark cross combination, after mature, branch point column harvest arranges the 1st row according to the 1st female parent,
2nd female parent arranges the 1st row, until n-th female parent arranges the 1st row, then the 1st female parent arranges the 2nd row, and the 2nd female parent arranges the 2nd row,
Until n-th female parent arranges the 2nd row, and so on arrive n-th female parent column Nth row.
Embodiment 1
Winter in 2016 implements 13 × 8 incomplete diallel cross formulated in combination work (Fig. 4) in In Xishuangbanna of Yunnan.
1) maternal group: 13 corn inbred lines (number: 16Y118-16Y130) of the new breeding of Chongqing Agricultural Academy of Science；
Male parent group: the corn selfing of backbone system Chongqing 1193, Chongqing 213, YA8201, LH8012 and 4 new breedings of Chongqing Agricultural Academy of Science
System's (number: 16Y114-16Y117).
2) each self-mating system in male parent group is taken, each male parent self-mating system plants 1 row, totally 8 row, and row is 7 meters long, 2 phase kinds of double strains point
16 nests are planted, the first phase and the maternal self-mating system same period plant 8 nests, plant the second phase after 3 days.
3) each self-mating system in maternal group is taken, each 1 column of female parent self-mating system plantation become 13 maternal column, Suo Youmu
The first row and the first row male parent self-mating system of this column self-mating system are planted in a row, the second row and second of all female parent column self-mating systems
Row male parent self-mating system is planted in a row, and so on.8 rows of each maternal column kind, a length of 1.7 meters of row of each maternal column, double strains
Plant 4 nests.
4) bagging before all self-mating system female fringes are spun, maternal self-mating system whole emasculation, set when male parent self-mating system tassel is bloomed
Bag takes powder, by the corresponding maternal column corresponding line self-mating system of pollinating of every row male parent self-mating system, i.e. all mothers of the 1st row male parent self-mating system pollination
1 row self-mating system of Ben Lie, all female parents of the 2nd row male parent self-mating system pollination arrange the 2nd row self-mating system, the pollination of the 3rd row male parent self-mating system
All female parents arrange the 3rd row self-mating system, and so on, all diallel cross test combinations are completed until preparing.
5) after pollinating, listing mark cross combination, after mature, branch point column harvest.The 1st is arranged according to the 1st female parent
Row, the 2nd female parent arrange the 1st row, until n-th female parent arranges the 1st row, then the 1st female parent arranges the 2nd row, and the 2nd female parent arranges the 2nd
Row, until n-th female parent arranges the 2nd row, and so on arrive n-th female parent column Nth row.
As a result: spring in 2017 carries out 13 × 8 incomplete diallel cross with the diallel cross combination prepared and tests, Cong Zhongjian
High-combining ability self-mating system 16NY135 is made, Chongqing D810 is named as, has passed through the expert appraisal of Agricultural in Chongqing committee orgaization,
Cross combination which has strong superior 16YN130 × 16YN115 is filtered out simultaneously, is named as Chongqing single 801, has passed through the southwestern spring of country in 2018
Corn regional test is participating in the southwestern Spring Maize Area examination of country in 2019.
Embodiment 2
16 × 8 diallel cross combination is assembled within 2017 using this method, specific experiment step is the same as embodiment 1.
Embodiment 3
18 × 8 diallel cross combination is assembled within 2018 using this method, specific experiment step is the same as embodiment 1.
To sum up, the preparation of diallel cross combination is carried out using this method, and simple, province is significantly embodied in practical operation
When, pollen be not easy the advantages of mixing.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not limitation with the present invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (4)
1. a kind of field planting method for efficiently assembling the combination of corn diallel cross, which comprises the following steps:
1) according to diallel cross test plan, male parent group self-mating system and maternal group self-mating system are determined；
2) male parent group self-mating system is taken, by male parent by row plantation, plants same male parent self-mating system, the line number etc. of male parent row with a line
In the species number of male parent self-mating system；
3) maternal group self-mating system is taken, by female parent by column plantation, the same maternal self-mating system of same row plantation, the columns etc. of female parent column
In the species number of maternal group self-mating system, line number is equal to the species number of male parent group self-mating system；And make the first row male parent self-mating system
In a row, the second row male parent and all maternal column the second row of self-mating system plantations are at one for first trip plantation with all maternal column self-mating systems
Row, and so on, until the most footline plantation of most footline male parent and all maternal column self-mating systems is in a row；
4) bagging before all self-mating system female fringes being spun, maternal self-mating system whole emasculation, bagging when male parent self-mating system tassel is bloomed
Powder is taken, by the corresponding pollination of every row male parent self-mating system to maternal column corresponding line self-mating system；
5) after pollinating, listing mark cross combination, after mature, branch point column harvest.
2. efficiently assembling the field planting method of corn diallel cross combination according to claim 1, which is characterized in that described
The row of each male parent row is long to be determined according to the maternal quantity of diallel cross test；Preferably, 16 nests are planted with the long double strains of 7 meters of rows.
3. efficiently assembling the field planting method of corn diallel cross combination according to claim 1, which is characterized in that described
Male parent group self-mating system was divided to for two phases sowed；The first phase and the maternal self-mating system same period, the second phase was than maternal self-mating system late sowing 3 days.
4. efficiently assembling the field planting method of corn diallel cross combination according to claim 1, which is characterized in that described
Cross combination sowing quantity needed for the long test according to diallel cross of the row of each maternal column determines；Preferably, with the long double strains of 1.7 meters of rows
Plant 4 nests.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910690444.5A CN110235777B (en) | 2019-07-29 | 2019-07-29 | High-efficiency field planting method for combining corn biserial hybridization combination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910690444.5A CN110235777B (en) | 2019-07-29 | 2019-07-29 | High-efficiency field planting method for combining corn biserial hybridization combination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110235777A true CN110235777A (en) | 2019-09-17 |
| CN110235777B CN110235777B (en) | 2020-09-22 |
Family
ID=67893744
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910690444.5A Expired - Fee Related CN110235777B (en) | 2019-07-29 | 2019-07-29 | High-efficiency field planting method for combining corn biserial hybridization combination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110235777B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112136684A (en) * | 2020-09-21 | 2020-12-29 | 连云港市农业科学院 | Efficient testing and matching method for corn line selection |
| CN113647321A (en) * | 2021-07-20 | 2021-11-16 | 河北省农林科学院旱作农业研究所 | Efficient and rapid detection method for corn inbred line combining ability |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507413A (en) * | 2009-04-01 | 2009-08-19 | 北京金色农华种业科技有限公司 | Corn hybrid breeding method |
| CN102138517A (en) * | 2010-12-17 | 2011-08-03 | 贵州省毕节地区农业科学研究所 | Breeding method for maize variety |
-
2019
- 2019-07-29 CN CN201910690444.5A patent/CN110235777B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101507413A (en) * | 2009-04-01 | 2009-08-19 | 北京金色农华种业科技有限公司 | Corn hybrid breeding method |
| CN102138517A (en) * | 2010-12-17 | 2011-08-03 | 贵州省毕节地区农业科学研究所 | Breeding method for maize variety |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112136684A (en) * | 2020-09-21 | 2020-12-29 | 连云港市农业科学院 | Efficient testing and matching method for corn line selection |
| CN113647321A (en) * | 2021-07-20 | 2021-11-16 | 河北省农林科学院旱作农业研究所 | Efficient and rapid detection method for corn inbred line combining ability |
| CN113647321B (en) * | 2021-07-20 | 2022-05-27 | 河北省农林科学院旱作农业研究所 | Efficient and rapid detection method for corn inbred line combining ability |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110235777B (en) | 2020-09-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101422133B (en) | Cabbage type rape self-incompatibility two-line hybrid seed-selection method | |
| CN110235777A (en) | A kind of field planting method efficiently assembling the combination of corn diallel cross | |
| CN107018891A (en) | A kind of rapidly and efficiently inexpensive method for breeding wheat | |
| CN102144538A (en) | Method for breeding high-oil-content rape variety | |
| CN105145334A (en) | Seed selection method of new rice species | |
| CN107018894B (en) | A kind of sugarcane crossbreeding selective nursery stage field experimental design method | |
| CN105075855A (en) | Method for breeding male sterility restoring line of yellow sprout pepper cytoplasm | |
| CN1994059A (en) | Three-step method for purification of Dian-1 type japonica sterile line stock seed | |
| CN101461325B (en) | Method for producing original seed for repeated reproduction of japonica rice three-line sterile line strain | |
| CN108633660B (en) | A kind of Radix Salviae Miltiorrhizae new varieties production of hybrid seeds method for culturing seedlings | |
| CN107660462B (en) | Method for creating hot pepper cytoplasmic male sterile line and simultaneously matching three lines | |
| CN104026004B (en) | A kind of resistance to bolting spring wild cabbage breeding method | |
| CN109156341A (en) | A method of Maize hybridized combination confecting efficiency is improved using more color pollination bags | |
| CN106688876A (en) | Combining and matching method of maize | |
| CN111406644B (en) | Cluster-growing pod pepper nuclear-cytoplasmic interaction type male sterile line breeding method and application of three-line matched system | |
| CN110352847B (en) | Rapid breeding and combined testing and matching identification method for cotton nuclear male sterile line | |
| CN1147218C (en) | Method for cultivating pomelo with seed-useless fruit | |
| CN105961187B (en) | A kind of selection of the polygonal cabbage type rape cytoplasmic male sterility restorer of main inflorescence | |
| CN104871957A (en) | Wheat chemical emasculation three-stage combined seed production method | |
| CN109430047A (en) | A kind of selection of drought tolerant corn self-mating system | |
| Financial System Inquiry (Australia: 2014) et al. | Final report | |
| CN114303936B (en) | Method for efficiently cultivating light-temperature-sensitive two-line hybrid wheat variety | |
| CN102172221A (en) | Method for breeding triple recessive genic male sterility temporary maintaining line of rape by utilizing culture of microspores | |
| CN1943319B (en) | Method for cross breeding seed of wheat | |
| CN105532425A (en) | Method for cultivating cauliflower hybrid by using male sterile line |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200922 Termination date: 20210729 |