CN1994059A - Three-step method for purification of Dian-1 type japonica sterile line stock seed - Google Patents
Three-step method for purification of Dian-1 type japonica sterile line stock seed Download PDFInfo
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- CN1994059A CN1994059A CNA2006100106088A CN200610010608A CN1994059A CN 1994059 A CN1994059 A CN 1994059A CN A2006100106088 A CNA2006100106088 A CN A2006100106088A CN 200610010608 A CN200610010608 A CN 200610010608A CN 1994059 A CN1994059 A CN 1994059A
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Abstract
The invention relates to a three-step method of I-type sterility rice, wherein it comprises that: selecting plant, checking, and cultivating. The invention can improve seed purity, hold former characters and shorten cultivate time.
Description
Technical field
The present invention relates to " three-step approach " that a kind of Yunnan I type japonica rice sterile original seed is purified, belong to agriculture field, more particularly, belong to the seed production field.
Background technology
It is the effective way of guaranteeing the hybrid rice increasing both production and income that the parent purifies.Male sterile line is the basis of hybrid rice, and the height of male sterile line purity is directly connected to hybrid seed purity, heterotic giving full play to and the service life of hybrid combination on producing.China is since promoting hybrid rice, and various places are attached great importance to the purification of male sterile line and prevented that assorted guarantor is pure, and it is that round-robin method, level Four seed production routine, select methods such as three garden methods that present method of purification mainly contains strain.
Yunnan I type male sterile line is that China uses one of rice sterile line the earliest now, and the sterile matter source transformation of finding with nineteen sixty-five has gone out numerous male sterile lines, has bred nearly thousand sterile line material.Yunnan I type male sterile line pollen abortion mainly is at two nuclear later stage and three nuclear initial stages period, and the partial starch accumulation is arranged, and pollen grain is not of uniform size, meets I-KI solution generation understain colour response, and pollen grain mostly is and dyes scum of a community's type; The pollen abortion type is one of classification foundation of male sterible series of rice, also is to produce one of test stone that goes up dissimilar male sterile line purification and rejuvenation and original seed production.
It is the method that adopts " individual plant in pairs, continuous backcross breeding male sterile lines " that traditional Yunnan I type japonica rice sterile original seed is purified, the five generations seeding in a large number of need backcrossing, the time cycle is long, can not prevent effectively assorted protect pure, the seed purity of purifying is not high, is seriously restricting the development of Japonica Hybrid.At present, also do not have a kind of technology perfect, improve the method for purification of Yunnan I type japonica rice sterile original seed purity effectively.
Summary of the invention
Main purpose of the present invention is the deficiency that overcomes existing male sterile line original seed purification technique method, a kind of Yunnan I type japonica rice sterile original seed purification seed purity height is provided, keep original coordinate force of male sterile line and typical proterties, shortened the seed productive life, reduced the method for reproductive order of generation.
The present invention adopts " three-step approach " of " choosing, mirror, numerous ", i.e. the male sterile line original seed method of purification of " selecting individual plant, identify strain system, breeding original seed ".Be specially:
1, selects individual plant
In the garden of backcrossing of isolate in strictness, paddy seedling being planted, male sterile line and maintenance line booting stage, at first select male sterile line, the some individual plants of maintenance line to mark the numbering of listing in the field according to the typical feature of former kind.At male sterile line heading stage, select good male sterile line individual plant to carry out pollen microscopic examination according to the typical proterties of male sterile line, the quantity that counting dyes and loses, circle loses, allusion quotation loses, the maintenance line individual plant of while microscope inspection and male sterile line equal number.According to pollen microscope inspection result; the final election sterile degree reaches the maintenance line individual plant that 100% male sterile line individual plant and normal pollen rate reach more than 90% and carries out pairing backcrossing; be to carry out test cross with these male sterile lines with recovering simultaneously, numbering results seed is in pairs joined breeding for planting evaluation next year and enlarging hand.
2, identify strain system
The pairing backcrossing seed that to gather in the crops last year is sowed seedling respectively, plants into strain nursery, carries out strain system and identifies; The test cross seed kind of corresponding test cross gained becomes the sub-district.Investigate typicalness, uniformity, breeding time and the pollen fertility of male sterile line; investigate the recovery situation of test cross kind simultaneously; system chooses to strain; find to have in the male sterile line colony individual plant of normal pollen and suspicious strain and test cross kind recovery extent poor; the strain system that hybrid vigour is not strong; then this strain system all eliminates, and the strain that selection has this male sterile line typical case proterties is that hand is joined expansion, mixes and receives breeder's stock.
3, breeding original seed
With strain is that hand is joined the breeder's stock that enlarge to mix results and was used for the breeding male sterile lines original seed on the 3rd year, begin by root and stem of certain plants inspection from seeding stage, tillering stage, fringe initial stage beginning breeding stock Tanaka, the different assorted strain of strict removal, duration of flowering is removed the plant of anther dehiscence loose powder, avoid " the string powder " of different assorted strain, be strictly on guard against mechanical admixture.
The invention has the beneficial effects as follows by microscope inspection male sterile line pollen abortion situation, can when determining to backcross individual plant, get rid of the different assorted strain of " can educate ", and make the abortion degree of the sterile strain of pairing backcrossing consistent relatively, but also can enlarge the production scale of original seed as required; Adopt " three-step approach " purification ratio to adopt the speed of " paired, the continuous breeding male sterile lines of individual plant " faster, owing to just can enter original seed production behind the first backcross generation, shortened the seed productive life, reduce reproductive order of generation, thus reach " high starting point, anti-assorted tight, quantity is big, program is simple " purpose.The original seed of purifying, the test cross checking through coordinate force can enter the large tracts of land crossbreeding after three seasons, reaches and shortens the seed productive life, reduces the original seed farm link, reduces reproductive order of generation, has kept original coordinate force of male sterile line and typical proterties effectively.
Embodiment
Embodiment one
Adopt " three-step approach " to purify, promptly select individual plant, identify strain system, breed original seed.In the garden of backcrossing of isolate in strictness, paddy seedling being planted, male sterile line and maintenance line booting stage, at first select male sterile line, the some individual plants of maintenance line to mark the numbering of listing in the field according to the typical feature of former kind.At male sterile line heading stage, select good male sterile line individual plant to carry out pollen microscopic examination according to the typical proterties of male sterile line, the quantity that counting dyes and loses, circle loses, allusion quotation loses, the maintenance line individual plant of while microscope inspection and male sterile line equal number.According to pollen microscope inspection result; the final election sterile degree reaches 50 of the maintenance line individual plants that 50 of 100% male sterile line individual plants and normal pollen rate reach more than 90% and carries out pairing backcrossing; be to carry out test cross with these male sterile lines with recovering simultaneously, numbering results seed is in pairs joined breeding for planting evaluation next year and enlarging hand.
The pairing backcrossing seed that to gather in the crops last year is sowed seedling respectively, plants into strain nursery, carries out strain system and identifies; The test cross seed kind of corresponding test cross gained becomes the sub-district.Investigate typicalness, uniformity, breeding time and the pollen fertility of male sterile line; investigate the recovery situation of test cross kind simultaneously; system chooses to strain; find to have in the male sterile line colony individual plant of normal pollen and suspicious strain and test cross kind recovery extent poor; the strain system that hybrid vigour is not strong; then this strain system all eliminates, and the strain that selection has this male sterile line typical case proterties is that hand is joined expansion, mixes and receives breeder's stock.
With strain is that hand is joined the breeder's stock that enlarge to mix results and was used for the breeding male sterile lines original seed on the 3rd year, begin by root and stem of certain plants inspection from seeding stage, tillering stage, fringe initial stage beginning breeding stock Tanaka, the different assorted strain of strict removal, duration of flowering is removed the plant of anther dehiscence loose powder, avoid " the string powder " of different assorted strain, be strictly on guard against mechanical admixture.
Adopt the method for " individual plant in pairs, continuous backcross breeding male sterile lines " to compare, the original seed of breeding is identified through field planting purity, be the results are shown in Table 1.
Table 1 three-step approach purification male sterile line original seed field planting purity qualification result
| Handle | The time that original seed is used to produce (year) | Field planting purity (%) | Comparison is according to improving percentage (%) |
| Three-step approach is purified | 3 | 99.91 | 0.45 |
| Individual plant is paired, continuous backcross (contrast) | 5 | 99.46 |
Embodiment two
Embodiment is identical with example one; the final election sterile degree reaches 100 of the maintenance line individual plants that 100 of 100% male sterile line individual plants and normal pollen rate reach more than 90% and carries out pairing backcrossing; be to carry out test cross with these male sterile lines with recovering simultaneously, numbering results seed is in pairs joined breeding for planting evaluation next year and enlarging hand.Adopt the method for " individual plant in pairs, continuous backcross breeding male sterile lines " to compare, the original seed of breeding is identified through field planting purity, be the results are shown in Table 2.
Table 2 three-step approach purification male sterile line original seed field planting purity qualification result
| Handle | The time that original seed is used to produce (year) | Field planting purity (%) | Comparison is according to improving percentage (%) |
| Three-step approach is purified | 3 | 99.92 | 0.44 |
| Individual plant is paired, continuous backcross (contrast) | 5 | 99.48 |
Embodiment three
Embodiment is identical with example one; the final election sterile degree reaches 150 of the maintenance line individual plants that 150 of 100% male sterile line individual plants and normal pollen rate reach more than 90% and carries out pairing backcrossing; be to carry out test cross with these male sterile lines with recovering simultaneously, numbering results seed is in pairs joined breeding for planting evaluation next year and enlarging hand.Adopt the method for " individual plant in pairs, continuous backcross breeding male sterile lines " to compare, the original seed of breeding is identified through field planting purity, be the results are shown in Table 3.
Table 3 three-step approach purification male sterile line original seed field planting purity qualification result
| Handle | The time that original seed is used to produce (year) | Field planting purity (%) | Comparison is according to improving percentage (%) |
| Three-step approach is purified | 3 | 99.90 | 0.52 |
| Individual plant is paired, continuous backcross (contrast) | 5 | 99.38 |
Embodiment four
Embodiment is identical with example one; the final election sterile degree reaches 200 of the maintenance line individual plants that 200 of 100% male sterile line individual plants and normal pollen rate reach more than 90% and carries out pairing backcrossing; be to carry out test cross with these male sterile lines with recovering simultaneously, numbering results seed is in pairs joined breeding for planting evaluation next year and enlarging hand.Adopt the method for " individual plant in pairs, continuous backcross breeding male sterile lines " to compare, the original seed of breeding is identified through field planting purity, be the results are shown in Table 4.
Table 4 three-step approach purification male sterile line original seed field planting purity qualification result
| Handle | The time that original seed is used to produce (year) | Field planting purity (%) | Comparison is according to improving percentage (%) |
| Three-step approach is purified | 3 | 99.92 | 0.45 |
| Individual plant is paired, continuous backcross (contrast) | 5 | 99.47 |
From top 4 case study on implementation as can be seen, the present invention has increased substantially the purity of Yunnan I type japonica rice sterile original seed, adopt " three-step approach " to purify, promptly select individual plant, identify that strain is, the method for purification of breeding original seed, original seed purity is 99.90～99.92%, and the original seed purity 99.38～99.48% that comparison is purified according to the method that adopts " individual plant in pairs, continuous backcross breeding male sterile lines " has improved 0.44%～0.52%.
Claims (1)
1, the purification " three-step approach " of a kind of Yunnan I type japonica rice sterile original seed, the step of this method is:
1., select individual plant
Isolate in strictness, in the garden of backcrossing that paddy seedling is planted, male sterile line and maintenance line booting stage, at first select male sterile line according to the typical feature of former kind, the some individual plants of maintenance line are marked in the field, the numbering of listing, at male sterile line heading stage, select good male sterile line individual plant to carry out pollen microscopic examination according to the typical proterties of male sterile line, counting dyes and loses, circle loses, the quantity that allusion quotation loses, the maintenance line individual plant of while microscope inspection and male sterile line equal number, according to pollen microscope inspection result, the final election sterile degree reaches the maintenance line individual plant that 100% male sterile line individual plant and normal pollen rate reach more than 90% and carries out pairing backcrossing, be to carry out test cross with these male sterile lines with recovering simultaneously, numbering results seed is in pairs joined breeding for planting evaluation next year and enlarging hand;
2., identify strain system
The pairing backcrossing seed that to gather in the crops last year is sowed seedling respectively, plants into strain nursery, carries out strain system and identifies; The test cross seed kind of corresponding test cross gained becomes the sub-district, investigate typicalness, uniformity, breeding time and the pollen fertility of male sterile line, investigate the recovery situation of test cross kind simultaneously, system chooses to strain, find to have in the male sterile line colony individual plant of normal pollen and suspicious strain and test cross kind recovery extent poor, the strain system that hybrid vigour is not strong, then this strain system all eliminates, the strain that selection has this male sterile line typical case proterties is that hand is joined expansion, mixes and receives breeder's stock;
3., breeding original seed
With strain is that hand is joined the breeder's stock that enlarge to mix results and was used for the breeding male sterile lines original seed on the 3rd year, begin by root and stem of certain plants inspection from seeding stage, tillering stage, fringe initial stage beginning breeding stock Tanaka, the different assorted strain of strict removal, duration of flowering is removed the plant of anther dehiscence loose powder, and the seed of results is original seed.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2006100106088A CN1994059A (en) | 2006-01-06 | 2006-01-06 | Three-step method for purification of Dian-1 type japonica sterile line stock seed |
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| CNA2006100106088A CN1994059A (en) | 2006-01-06 | 2006-01-06 | Three-step method for purification of Dian-1 type japonica sterile line stock seed |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101461325B (en) * | 2009-01-23 | 2011-11-16 | 天津市农作物研究所 | Method for producing original seed for repeated reproduction of japonica rice three-line sterile line strain |
| CN102599048A (en) * | 2012-03-28 | 2012-07-25 | 云南农业大学 | Breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding |
| CN103609434A (en) * | 2013-11-25 | 2014-03-05 | 广西壮族自治区农业科学院水稻研究所 | High-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds |
| CN104798680A (en) * | 2015-05-14 | 2015-07-29 | 重庆市农业科学院 | Fragrant high-quality hybrid rice advantage maintaining method |
| CN107396827A (en) * | 2016-05-19 | 2017-11-28 | 上海天谷生物科技股份有限公司 | A kind of saving water, resisting drought rice CMS line purifies propagation method |
-
2006
- 2006-01-06 CN CNA2006100106088A patent/CN1994059A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101461325B (en) * | 2009-01-23 | 2011-11-16 | 天津市农作物研究所 | Method for producing original seed for repeated reproduction of japonica rice three-line sterile line strain |
| CN102599048A (en) * | 2012-03-28 | 2012-07-25 | 云南农业大学 | Breeding method of japonica type new sterile cytoplasmic source sterile line for hybrid japonica rice breeding |
| CN103609434A (en) * | 2013-11-25 | 2014-03-05 | 广西壮族自治区农业科学院水稻研究所 | High-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds |
| CN104798680A (en) * | 2015-05-14 | 2015-07-29 | 重庆市农业科学院 | Fragrant high-quality hybrid rice advantage maintaining method |
| CN107396827A (en) * | 2016-05-19 | 2017-11-28 | 上海天谷生物科技股份有限公司 | A kind of saving water, resisting drought rice CMS line purifies propagation method |
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