CN104521740A - Synchronous transform-breeding method for wuta-cai homozygous inbred line and cytoplasmic male sterile line - Google Patents
Synchronous transform-breeding method for wuta-cai homozygous inbred line and cytoplasmic male sterile line Download PDFInfo
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- CN104521740A CN104521740A CN201410788076.5A CN201410788076A CN104521740A CN 104521740 A CN104521740 A CN 104521740A CN 201410788076 A CN201410788076 A CN 201410788076A CN 104521740 A CN104521740 A CN 104521740A
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Abstract
The invention discloses a synchronous transform-breeding method for a wuta-cai homozygous inbred line and a cytoplasmic male sterile line. The synchronous transform-breeding method comprises the following steps of simultaneously carrying out inbred pollination at a bud stage and hybrid pollination at a flowering stage by taking a hybrid wuta-cai variety as an inbred line and a male sterile line as a sterile source; and carrying out a plurality of generations of inbred and hybrid synchronous transform-breeding to simultaneously obtain the homozygous inbred line and the stable wuta-cai cytoplasmic male sterile line. The method is a novel synchronous transform-breeding method; the homozygous inbred line and the stable cytoplasmic male sterile line can be simultaneously obtained after 4-5 generations, so that the breeding years are greatly shortened; and meanwhile the defect of variety degeneration of the inbred line is overcome, the method is simple, convenient and rapid in operation, and a great number of labors are saved.
Description
Technical field
What the present invention relates to is the technical field of Crop Genetic Breeding, the in particular synchronous transfer method of a kind of Wuta-tsai homozygous inbred lines and cytoplasmic male sterile line.
Background technology
In prior art, the selection of the cytoplasmic male sterile line of crop is generally: now inbred line selected pure, again the inbred line of isozygotying and sterile source are hybridized, backcross again, this method can be screened and be obtained stable male sterile line, but length consuming time, the general time needing about 10 years, is unfavorable for the quick application of cytoplasmic male sterile line.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide the synchronous transfer method of a kind of Wuta-tsai homozygous inbred lines and cytoplasmic male sterile line, to solve the technical problem of Wuta-tsai male-sterile line breeding time length.
The present invention is achieved by the following technical solutions:
A synchronous transfer method for Wuta-tsai homozygous inbred lines and cytoplasmic male sterile line, comprises the following steps:
(1) using heterozygote Wuta-tsai kind as inbred line, with the male sterile line kind of close relative's kind of Wuta-tsai for sterile source, a kind of inbred line proterties is selected to be the objective trait screened, gather the pollen of inbred line, column cap inbred line pollen being awarded the inbred line of other non-open bud carries out flower bud phase pollination self, obtains selfing F1 seed, simultaneously, inbred line pollen is awarded on the column cap of sterile source, carries out florescence hybridization pollination, obtain hybridization F1 seed;
(2) by selfing F1 seed and hybridization F1 planting seed, both sowing time consistency or sow sequentially, both guarantees florescence unanimously, obtain selfing F1 plant and hybridization F1 plant, gather selfing F1 plant pollen, selfing F1 plant pollen is awarded on the column cap of selfing F1 plant of other non-open bud, carry out flower bud phase pollination self, obtain selfing F2 seed, simultaneously, selfing F1 plant pollen is awarded on the column cap of the hybridization F1 plant consistent with objective trait, carries out florescence hybridization pollination, obtain hybridization F2 seed;
(3) offspring repeats step (2) and carries out selfing and hybridization, obtains selfing Fn seed, is the Wuta-tsai kind of isozygotying, and obtains hybridization Fn seed, is stable Wuta-tsai male sterile line kind.
Close relative's kind of the Wuta-tsai of described step (1) is selected from the one in a variety of Chinese cabbage, Chinese cabbage and Xiaoqinling Nature Reserve, the male sterile line cultivar origin of a variety of Chinese cabbage, Chinese cabbage and Xiaoqinling Nature Reserve is extensive, near with the affiliation of Wuta-tsai, easily and Wuta-tsai hybridize and obtain filial generation.
The heterozygote Wuta-tsai kind of described step (1) is selected from the one in Huainan radial striations, Hefei gold zone crow and little eight leaves in Shanghai.
The objective trait of described step (1) be selected from the leaf look of heterozygote Wuta-tsai kind, frame type and leaf in one or more.
The n of described step (3) was 4 ~ 5 generations.
The present invention has the following advantages compared to existing technology: the synchronous transfer method that the invention provides a kind of Wuta-tsai homozygous inbred lines and cytoplasmic male sterile line, the method is a kind of new method of synchronous transformation, only need through 4 ~ 5 generations, namely 5 ~ 6 years, the inbred line and stable cytoplasmic male sterile line of isozygotying can be obtained simultaneously, substantially reduce the breeding time limit; The present invention simultaneously adopts the mode of flower bud phase pollination self to reserve seed for planting, and overcomes the defect of inbred line deterioration of variety; The method is fast easy and simple to handle, does not need a large amount of manpowers to complete pollination, saves a large amount of manual labor.
Accompanying drawing explanation
Fig. 1 is the flow chart of synchronous selection of the present invention.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
Embodiment 1
The Wuta-tsai homozygous inbred lines of the present embodiment and the synchronous selection of cytoplasmic male sterile line, its flow process as shown in Figure 1, comprises the following steps:
(1) first and second year parent culture:
Select common heterozygote Wuta-tsai kind Hefei gold zone crow WS-03 to be inbred line parent, selection a variety of Chinese cabbage BA1 is the sterile source parent of male sterile line, selects leaf look and frame type to be objective trait.
In about 10 days September of First Year, be seeded in booth by above-mentioned two parent materials and carry out nursery, the order of sowing can have successively, ensures the flower synchronization of two parents, treats that seedling age is to about 35 days definite values to large Tanaka.Treat about 20 days December then, then carry out late growing stage by two parent's plantlet of transplant to booth.By the time the March of Second Year, carry out bagging to two parents of full-bloom stage, concrete steps are: plucked by the bud of having bloomed of inbred line and sterile source, then carry out bagging, within after bagging three days, pollinate.Described pollination comprises two kinds of modes, and one is florescence pollination, and the column cap awarding sterile source by the pollen in inbred line bag is hybridized, and obtains hybridization F1 seed; Another kind is bud pollination, award on the column cap of the inbred line of other non-open bud by the pollen in inbred line bag, then bagging is continued, object is in order to selfing is reserved seed for planting, the mode of this bud pollination can make the heterozygote genotype of inbred line be isozygotied, after bearing pods, to pinch bag to the spray in bag, results obtain selfing F1 seed.Hybridization F1 seed and selfing F1 seed are placed in 4 DEG C of Storage in refrigerator stand-by.
Back cross breeding in (2) the 3rd years:
In about 10 days September of Second Year, the hybridization F1 seed retain above-mentioned steps (1) then and selfing F1 seed continue seeding and seedling raising, obtain hybridization F1 plant and selfing F1 plant, until nourish and grow during the later stage, eliminate the hybridization F1 plant inconsistent with target shape, obtain the hybridization F1 plant after screening.Treat full-bloom stage, bagging is carried out to the plant of full-bloom stage, the same step of concrete steps (1), bud of blooming by the hybridization F1 plant after selfing F1 plant and screening is plucked, then carry out bagging, within after bagging three days, pollinate, described Pollination Modes comprises: the florescence pollinates, award by the pollen in selfing F1 plant bag on the column cap of hybridizing F1 plant and hybridize, obtain hybridization F2 seed; Bud pollination, awards on the column cap of selfing F1 plant of other non-open bud by the pollen in selfing F1 plant bag, then continues bagging, and after bearing pods, to pinch bag to the spray in bag, results obtain selfing F2 seed.Hybridization F2 seed and selfing F2 seed are placed in 4 DEG C of Storage in refrigerator stand-by.
Back cross breeding in (3) 4th ~ 5 years:
Repeat the back cross breeding step 2 time of step (2), obtain hybridization F4 seed and selfing F4 seed, described hybridization F4 seed is stable male sterile line, called after 4W-BA1-03, described selfing F4 seed is homozygous inbred lines, breeding time is 5 years, needs the time of about 10 years compared to tradition, and its breeding time is considerably reduced.
Embodiment 2
The Wuta-tsai homozygous inbred lines of the present embodiment and the synchronous selection of cytoplasmic male sterile line, wherein, the back cross breeding step of step (2) repeats 3 times, obtain hybridization F5 seed and selfing F5 seed, be stable male sterile line and pure and mild inbred line, described stable male sterile line called after 5W-BA1-03, breeding time is 6 years, and other step is with embodiment 1.
Add up the sterile proterties of the male sterile line kind that embodiment 1 and embodiment 2 obtain, result is as shown in table 1 below:
Table 1: the sterility statistics of the male sterile line kind of embodiment 1 and 2
Can find out in table 1, after synchronous transfer method transformation 4 ~ 5 generation of the present invention, sterile plant rate and the sterile degree of the male sterile line kind of its Wuta-tsai obtained all reach 100%, illustrate that male-sterile character is all got off by genetic stability.
Embodiment 3
The present embodiment is the homozygous inbred lines of Huainan radial striations and the synchronous selection of male sterile line, step comprises: select Huainan radial striations WS-01 to be inbred line parent, selection Chinese cabbage BA7 is the sterile source parent of male sterile line, select leaf look and frame type and leaf be objective trait, other steps are with embodiment 1, through back cross breeding in continuous 5 years, obtain Wuta-tsai homozygous inbred lines kind and male sterile line kind 4W-BA7-01.
Embodiment 4
The present embodiment is the homozygous inbred lines of little eight leaves in Shanghai and the synchronous selection of male sterile line, step comprises: select the little eight leaf WS-06 in Shanghai to be inbred line parent, select the sterile source parent that Xiaoqinling Nature Reserve summer hat (numbering BA3) is male sterile line, leaf look is selected to be objective trait, other steps are with embodiment 2, through back cross breeding in continuous 6 years, obtain Wuta-tsai homozygous inbred lines kind and male sterile line kind 5W-BA3-06.
Claims (5)
1. a synchronous transfer method for Wuta-tsai homozygous inbred lines and cytoplasmic male sterile line, is characterized in that, comprise the following steps:
(1) using heterozygote Wuta-tsai kind as inbred line, with the male sterile line kind of close relative's kind of Wuta-tsai for sterile source, a kind of inbred line proterties is selected to be the objective trait screened, gather the pollen of inbred line, column cap inbred line pollen being awarded the inbred line of other non-open bud carries out flower bud phase pollination self, obtains selfing F1 seed, simultaneously, inbred line pollen is awarded on the column cap of sterile source, carries out florescence hybridization pollination, obtain hybridization F1 seed;
(2) by selfing F1 seed and hybridization F1 planting seed, obtain selfing F1 plant and hybridization F1 plant, gather selfing F1 plant pollen, selfing F1 plant pollen is awarded on the column cap of selfing F1 plant of other non-open bud, carries out flower bud phase pollination self, obtain selfing F2 seed, simultaneously, selfing F1 plant pollen is awarded on the column cap of the hybridization F1 plant consistent with objective trait, carries out florescence hybridization pollination, obtain hybridization F2 seed;
(3) offspring repeats step (2) and carries out selfing and hybridization, obtains selfing Fn seed, is the Wuta-tsai kind of isozygotying, and obtains hybridization Fn seed, is stable Wuta-tsai male sterile line kind.
2. the synchronous transfer method of a kind of Wuta-tsai homozygous inbred lines according to claim 1 and cytoplasmic male sterile line, is characterized in that, close relative's kind of the Wuta-tsai of described step (1) is selected from the one in a variety of Chinese cabbage, Chinese cabbage and Xiaoqinling Nature Reserve.
3. the synchronous transfer method of a kind of Wuta-tsai homozygous inbred lines according to claim 1 and cytoplasmic male sterile line, it is characterized in that, the heterozygote Wuta-tsai kind of described step (1) is selected from the one in Huainan radial striations, Hefei gold zone crow and little eight leaves in Shanghai.
4. the synchronous transfer method of a kind of Wuta-tsai homozygous inbred lines according to claim 1 and cytoplasmic male sterile line, it is characterized in that, the objective trait of described step (1) be selected from the leaf look of heterozygote Wuta-tsai kind, frame type and leaf in one or more.
5. the synchronous transfer method of a kind of Wuta-tsai homozygous inbred lines according to claim 1 and cytoplasmic male sterile line, is characterized in that, the n of described step (3) was 4 ~ 5 generations.
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Cited By (3)
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| CN106258941A (en) * | 2016-09-21 | 2017-01-04 | 韶关学院 | The selection-breeding of a kind of Wuta-tsai and propagation method |
| CN106613898A (en) * | 2016-09-21 | 2017-05-10 | 韶关学院 | Breeding method of Wuta-tsai cytoplasmic male sterile line and maintainer line |
| CN109566401A (en) * | 2019-01-14 | 2019-04-05 | 安徽农业大学 | The B. campestris L.ssp. Chinensis extremely homozygous inbred lines of resistance to bolting and self incompatible line quick breeding method |
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| WO2001022805A1 (en) * | 1999-09-28 | 2001-04-05 | University Of Delhi, South Campus | Stable cytoplasmic male sterile brassica campestris plant which contain 'polima' cytoplasm and method for obtaining such plants |
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| CN103461100A (en) * | 2013-08-26 | 2013-12-25 | 安徽省农业科学院园艺研究所 | Purple Wuta-tsai breeding method |
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| WO2001022805A1 (en) * | 1999-09-28 | 2001-04-05 | University Of Delhi, South Campus | Stable cytoplasmic male sterile brassica campestris plant which contain 'polima' cytoplasm and method for obtaining such plants |
| CN103444515A (en) * | 2013-08-26 | 2013-12-18 | 安徽省农业科学院园艺研究所 | Transfer method for male sterile line of purple wuta-tsai |
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Cited By (3)
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| CN106258941A (en) * | 2016-09-21 | 2017-01-04 | 韶关学院 | The selection-breeding of a kind of Wuta-tsai and propagation method |
| CN106613898A (en) * | 2016-09-21 | 2017-05-10 | 韶关学院 | Breeding method of Wuta-tsai cytoplasmic male sterile line and maintainer line |
| CN109566401A (en) * | 2019-01-14 | 2019-04-05 | 安徽农业大学 | The B. campestris L.ssp. Chinensis extremely homozygous inbred lines of resistance to bolting and self incompatible line quick breeding method |
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