CN109156341A - A method of Maize hybridized combination confecting efficiency is improved using more color pollination bags - Google Patents
A method of Maize hybridized combination confecting efficiency is improved using more color pollination bags Download PDFInfo
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- CN109156341A CN109156341A CN201811367750.7A CN201811367750A CN109156341A CN 109156341 A CN109156341 A CN 109156341A CN 201811367750 A CN201811367750 A CN 201811367750A CN 109156341 A CN109156341 A CN 109156341A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention belongs to agricultural crops Biotechnology in Genetic Breeding fields, more particularly to a kind of method for improving Maize hybridized combination confecting efficiency using more color pollination bags, Maize hybridized combination confecting efficiency is improved within the limited time at corn florescence, step is carried out to the technical essential of corn artificial bagging pollination key link disassemble and implement step by step by more color pollination bags label across the period, this method can carry out multiple male parent Testers progress cross combination preparations simultaneously for corn breeding worker and provide reference, be suitble to the preparation of a large amount of Maize hybridized combinations.The present invention compares the crossover operation activity time and personnel's contradiction for efficiently solving that Testers male parent florescence limited time and scene are manually listed when breeder carries out Maize hybridized combination preparation scene pollination on a large scale compared to existing traditional pollination technique, with following remarkable advantage: within the identical time at male parent florescence, corn inbred line cross combination confecting efficiency promotes 3 times or more under the conditions of same personnel amount, labour cost.
Description
Technical field
The invention belongs to agricultural crops Biotechnology in Genetic Breeding fields, and in particular to a kind of to improve jade using more color pollination bags
The method of rice cross combination confecting efficiency.
Background technique
Corn hybrid seed has reached developed country's level in the popularity rate of China.The basis of hybrid maize breeding and key
It is the self-mating system for obtaining high-purity, artificial bagging pollination is the key that corn inbred lines, purificates and rejuvenates and assemble Combination nova
Link.Corn florescence pollination window phase limited time, artificial pollination core process directly influence Maize hybridized combination preparation
Efficiency.A set of both simple reality is explored and made to adaptation to local conditions to the limitation for how overcoming Maize hybridized combination confecting efficiency low
It is of great significance with the bagging pollination technique of efficient quality guaranteeing again.
In recent years, corn breeding worker also successively carried out the research of Maize hybridized combination configuration method, such as Wang Zhiwu
A kind of patent of invention " preparation method of Maize hybridized combination " of equal applications, 108184654 A of application publication number CN, but the party
Method is only adapted to single male parent Testers isolated area and assembles, and can not adapt to more male parent Testers while carry out hybridization to assemble, and easily
Mix by the numerous periphery field external source pollen string powder of South of Hainan, this method is centainly restricted in terms of practical application；Such as Shi Chen
A kind of 207118438 U of utility model patent " corn pollination device " Authorization Notice No. CN of equal applications and the palace Shen Ming Bodeng
207411114 U of utility model patent " a kind of manual corn pollination device " Authorization Notice No. CN please, adopts zasiokaurin
Collection, pollinating device are designed and produced, and can not solve the more male parent Testers in corn breeding man field while carry out hybridization to assemble
Task.
Academy of Agri-Science and Technology Anhui Province corn germ plasm resource team Hainan breeding in Hainan member is for many years in corn preparing hybrid
Combined aspects have accumulated propagable breeding results and practical experience, miscellaneous in order to improve corn within the limited time at corn florescence
Formulated in combination efficiency is handed over, we have carried out step to the technical essential of corn artificial bagging pollination key link and have disassembled and pass through more
Color pollination bag label is implemented step by step across the period, and this method can carry out multiple male parent tests simultaneously for corn breeding worker
It kind carries out cross combination and prepares to provide reference, be suitble to the preparation of a large amount of Maize hybridized combinations, with stable embodiment and answer
With value.
Summary of the invention
The purpose of the present invention: breeder needs when carrying out hybridization pollination in corn Hybrid breeding in commercial system after self-mating system bagging
It completes on the sheepskin paper bag of pollination or the information such as plastics listed subscript note Parent pedigree source, date comes clear pedigree source
Convenient for registering when harvest, but there is Testers male parent florescence limited time and live people in the conventional method in actual use
The listed crossover operation activity time contradiction of work, time-consuming and laborious and inefficiency.The corn florescence is short, and corn inbred line just matches test cross
Heavy workload, therefore the method for being badly in need of finding raising Maize hybridized combination confecting efficiency has to solve the time at Testers male parent florescence
The crossover operation activity time contradiction and artificial intensive high cost problem that limit, scene are manually listed.
The present invention is achieved by the following technical solutions: a kind of to be matched using more color pollination bags raising Maize hybridized combination
The method of efficiency processed, including the following contents:
(1) paternal plant to be test successively is labeled as Aj(j=1,2 ..., m by the assortment of Testers), different cultivars
Paternal plant Aj be provided with the flour bag of different colours label, the color of corresponding with paternal plant Aj flour bag label is Cj；
(2) maternal plant to be test successively is labeled as Bk(k=1,2 ..., n by assortment), in the female parent of each kind
Single plant female fringe to be pollinated is chosen in plant Bk respectively, the single plant female fringe of n different cultivars forms maternal experimental group, each female parent
Experimental group keeps uniline plantation, and each female parent experimental group is corresponding in turn to different paternal plant Aj, in different maternal experimental groups
Single plant female fringe to be pollinated is labeled as Bkj；
(3) single plant female fringe Bkj is provided with the bagging that different colours mark in each maternal experimental group, corresponding with paternal plant Aj
Maternal experimental group in single plant female fringe Bkj bagging label color it is consistent with the flour bag marker color of paternal plant Aj；
(4) information labels that each single plant female fringe Bkj is printed using label machine are awarded in corn inbred line cross combination preparation
After powder task, by information labels hanging buckle on corresponding single plant female fringe Bkj.
As further improvement of these options, the flour bag and bagging are the sheepskin paper bag with color mark.
As further improvement of these options, the color mark is to dye to obtain by sheepskin paper bag.
As further improvement of these options, when pollinating to the single plant female fringe Bkj in maternal experimental group wait pollinate, directly
The pollen for receiving collection flour bag marker color paternal plant Aj identical with single plant female fringe Bkj bagging marker color is completed single plant and is awarded
Powder is not necessarily to hanging buckle information labels in pollination.
As further improvement of these options, the information labels are single plant female fringe Bkj cross combination pedigree source
Information, the kind information of kind information and maternal plant Bk including the corresponding paternal plant Aj of single plant female fringe Bkj.
Wherein m and n is the natural number greater than zero, is arranged according to actual experiment demand；Experiment row is long according to paternal plant
Aj, the numerical value of maternal plant Bk and kind are configured.
The present invention carries out Maize hybridized combination compared to efficiently solving breeder compared to existing traditional pollination technique on a large scale
The crossover operation activity time and personnel's lance that Testers male parent florescence limited time and scene are manually listed when the pollination of preparation scene
Shield has following remarkable advantage:
(1) within the identical time at male parent florescence, corn inbred line cross combination is matched under the conditions of same personnel amount, labour cost
3 times of improved efficiency processed or more；
(2) breeder pollinates, and work implementation is convenient, and corresponding flour bag marker color takes paternal plant Aj pollen to complete corresponding color mark
Remember the single plant female fringe Bkj pollination of bagging, then hanging buckle after the information labels of the corresponding each single plant female fringe Bkj of printing, simplifies jade
Rice cross combination preparation process.
Detailed description of the invention
Fig. 1 is that paternal plant Aj of the invention corresponds to single plant female fringe Bkj cross combination preparation schematic diagram.
Fig. 2 is that South of Hainan numerous base tradition pollination bag (white bag) Maize hybridized combination prepares photo.
Fig. 3 is that the numerous base of South of Hainan more color pollination bags (color bag) Maize hybridized combination prepares photo.
Specific embodiment
For the technology contents that the present invention will be described in detail, the purpose and effect realized, below in conjunction with attached drawing and embodiment party
Formula is explained.
Wherein bagging color includes the colors such as green, yellow, purple, pink colour, blue, red in Fig. 3, is shown not in figure
Obviously.
The most critical design of the present invention is: being prepared to improve Maize hybridized combination within the limited time at corn florescence
Efficiency has carried out step to the technical essential of corn artificial bagging pollination key link and has disassembled and by more color pollination bags label
It is implemented step by step across the period, this method can carry out multiple male parent Testers for corn breeding worker simultaneously and carry out cross combination
It prepares and reference is provided, be suitble to the preparation of a large amount of Maize hybridized combinations, there is stable embodiment and application value.
As shown in Figure 1-3, a kind of method for improving Maize hybridized combination confecting efficiency using more color pollination bags, including with
Lower content:
(1) paternal plant to be test successively is labeled as Aj(j=1,2 ..., m by the assortment of Testers), different cultivars
Paternal plant Aj be provided with the flour bag of different colours label, the color of corresponding with paternal plant Aj flour bag label is Cj；
(2) maternal plant to be test successively is labeled as Bk(k=1,2 ..., n by assortment), in the female parent of each kind
Single plant female fringe to be pollinated is chosen in plant Bk respectively, the single plant female fringe of n different cultivars forms maternal experimental group, each female parent
Experimental group keeps uniline plantation, and each female parent experimental group is corresponding in turn to different paternal plant Aj, in different maternal experimental groups
Single plant female fringe to be pollinated is labeled as Bkj；
(3) single plant female fringe Bkj is provided with the bagging that different colours mark in each maternal experimental group, corresponding with paternal plant Aj
Maternal experimental group in single plant female fringe Bkj bagging label color it is consistent with the flour bag marker color of paternal plant Aj；
(4) information labels that each single plant female fringe Bkj is printed using label machine are awarded in corn inbred line cross combination preparation
After powder task, by information labels hanging buckle on corresponding single plant female fringe Bkj.
Wherein, the flour bag and bagging are the sheepskin paper bag with color mark；The color mark is by sheepskin paper bag
Dyeing obtains.
Wherein, the information labels are single plant female fringe Bkj cross combination pedigree source-information, including Bkj pairs of single plant female fringe
The kind information of the paternal plant Aj answered and the kind information of maternal plant Bk.
Single plant female fringe Bkj cross combination is corresponded to for paternal plant Aj in Fig. 1 and prepares schematic diagram, and each female parent experimental group is corresponding
Male parent Testers；Fig. 2 is that the numerous base tradition pollination bag of South of Hainan (white bag) Maize hybridized combination prepares photo, is white, difficult
To differentiate, need to be differentiated by information labels；Fig. 3 is the more color pollination bags in the numerous base of South of Hainan (color bag) corn hybridization group
It closes and prepares photo, when pollinating to the single plant female fringe Bkj in maternal experimental group wait pollinate, directly collect flour bag marker color and single plant
The pollen of the identical paternal plant Aj of female fringe Bkj bagging marker color completes single plant pollination, is not necessarily to hanging buckle information mark in pollination
Label.
Embodiment 1
It 2016, benefits the nation town Academy of Agri-Science and Technology Anhui Province corn research center breeding in Hainan positioned at Hainan Province Li Autonomous County of ledong
Base, corn germ plasm resource team Hainan breeding in Hainan member has been carried out using 9 kinds of color sheepskin paper bags to be pollinated using more colors
Bag improves the verification test of Maize hybridized combination confecting efficiency.
9 parts of male parent Testers, single Testers plant 10 rows, and point 2 phases sowing is spaced 5 day date of seeding, row is 6 meters long, every row kind
Plant 27 plants；Maternal 432 parts to be measured, maternal uniline plantation, row is 6 meters long, and every row plants 27 plants.Experimental field rotary cultivator rotation ground two times,
Row spacing row application is determined in ridge, every 667m2 applies 40 kilograms of nitrogen-phosphorus-potassium compound fertilizer (15-15-15), 10 kilograms of ﹢ urea or so,
60 jin of organic fertilizer, 0.6 meter of ridging row spacing, ridge is 0.3 meter high.It completes in ridging on October 30, waters after sowing November 2,
Exhibition 10-12 leaf phase mu imposes 20 kilograms of urea or so, per acre with+50% Acetochlor of 40% Atrazine before emerging after corn seeding
(75+75 milliliters) it is closed spraying to be watered 50 kilograms of progress.2.5% " decis " medical fluid of seedling stage, is made into 1500 times of medical fluids,
It eliminates " cutworm "；Trumpet period is mixed thoroughly to be sprinkled into horn mouth with 1.5% phoxim granule with 1:15 ratio and fine earth prevents and treats jade
Rice snout moth's larva.
Using the corresponding maternal single plant female fringe Bkj cross combination preparation principle of paternal plant Aj, opened for maternal 432 parts to be measured
Work, the breeding of corn germ plasm resource team South of Hainan are prepared in the cross combination for having opened up 9 parts of Testers (i.e. 9 kinds of color sheepskin paper bags)
Kind 2 people of member assembles 3623 parts of cross combination F1 (being unworthy of a underworker) phase altogether, and harvesting threshing on March 20th, 2017 is completed,
Paid 3597 parts of cross combination F1.Under same personnel, cost conditions, the numerous Maize hybridized combination of 2016-2017 year South of Hainan is matched
Quantity processed is 2.4 times that quantity is prepared in the cross combination of 2015-2016 year.
Embodiment 2
It 2017, benefits the nation town Academy of Agri-Science and Technology Anhui Province corn research center breeding in Hainan positioned at Hainan Province Li Autonomous County of ledong
Base, corn germ plasm resource team Hainan breeding in Hainan member has been carried out using 10 kinds of color sheepskin paper bags to be pollinated using more colors
Bag improves the verification test of Maize hybridized combination confecting efficiency.
10 parts of male parent Testers, single Testers plant 12 rows, and point 2 phases sowing is spaced 5 day date of seeding, row is 5.8 meters long, often
25 plants of row plantation；Maternal 579 parts to be measured, maternal uniline plantation, row is 5.8 meters long, and every row plants 25 plants.Experimental field rotary cultivator rotation ground
Two times, row spacing row application is determined in ridge, every 667m2 applies 10 kilograms of 40 kilograms+urea of nitrogen-phosphorus-potassium compound fertilizer (15-15-15)
Left and right, 60 jin of organic fertilizer, 0.6 meter of ridging row spacing, ridge is 0.3 meter high.It is completed in ridging on October 29, after November 1 was sowed
Watering, exhibition 10-12 leaf phase mu imposes 20 kilograms of urea or so, per acre with+50% second of 40% Atrazine before emerging after corn seeding
It is closed spraying to be watered 50 kilograms of progress for careless amine (75+75 milliliters).2.5% " decis " medical fluid of seedling stage, is made into 1500 times
Medical fluid is eliminated " cutworm "；Trumpet period is mixed thoroughly with 1:15 ratio and fine earth with 1.5% phoxim granule and is sprinkled into horn mouth
Prevent and treat corn borer.
Using the corresponding maternal single plant Bkj cross combination preparation principle of male parent Testers Aj, for maternal 579 parts of developments to be measured
Work, corn germ plasm resource team Hainan breeding in Hainan are prepared in the cross combination of 10 parts of Testers (10 kinds of color sheepskin paper bags)
2 people of member (being unworthy of underworker) assembles 5164 parts of cross combination F1 a phase altogether, and harvesting threshing on March 15th, 2018 is completed, real
Receive 5035 parts of cross combination F1.Under same personnel, cost conditions, the numerous Maize hybridized combination of 2017-2018 year South of Hainan is prepared
Quantity is 3.4 times that quantity is prepared in the cross combination of 2015-2016 year.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (5)
1. a kind of method for improving Maize hybridized combination confecting efficiency using more color pollination bags, which is characterized in that including following
Content:
(1) paternal plant to be test successively is labeled as Aj(j=1,2 ..., m by the assortment of Testers), different cultivars
Paternal plant Aj be provided with the flour bag of different colours label, the color of corresponding with paternal plant Aj flour bag label is Cj；
(2) maternal plant to be test successively is labeled as Bk(k=1,2 ..., n by assortment), in the female parent of each kind
Single plant female fringe to be pollinated is chosen in plant Bk respectively, the single plant female fringe of n different cultivars forms maternal experimental group, each female parent
Experimental group keeps uniline plantation, and each female parent experimental group is corresponding in turn to different paternal plant Aj, in different maternal experimental groups
Single plant female fringe to be pollinated is labeled as Bkj；
(3) single plant female fringe Bkj is provided with the bagging that different colours mark in each maternal experimental group, corresponding with paternal plant Aj
Maternal experimental group in single plant female fringe Bkj bagging label color it is consistent with the flour bag marker color of paternal plant Aj；
(4) information labels that each single plant female fringe Bkj is printed using label machine are awarded in corn inbred line cross combination preparation
After powder task, by information labels hanging buckle on corresponding single plant female fringe Bkj.
2. a kind of method for improving Maize hybridized combination confecting efficiency using more color pollination bags as described in claim 1, special
Sign is that the flour bag and bagging are the sheepskin paper bag with color mark.
3. a kind of method for improving Maize hybridized combination confecting efficiency using more color pollination bags as claimed in claim 2, special
Sign is that the color mark is to dye to obtain by sheepskin paper bag.
4. a kind of method for improving Maize hybridized combination confecting efficiency using more color pollination bags as described in claim 1, special
Sign is, when pollinating to the single plant female fringe Bkj in maternal experimental group wait pollinate, directly collects flour bag marker color and single plant female fringe
The pollen of the identical paternal plant Aj of Bkj bagging marker color completes single plant pollination, is not necessarily to hanging buckle information labels in pollination.
5. a kind of method for improving Maize hybridized combination confecting efficiency using more color pollination bags as described in claim 1, special
Sign is that the information labels are single plant female fringe Bkj cross combination pedigree source-information, including the corresponding father of single plant female fringe Bkj
The kind information of this plant Aj and the kind information of maternal plant Bk.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112136684A (en) * | 2020-09-21 | 2020-12-29 | 连云港市农业科学院 | Efficient testing and matching method for corn line selection |
| CN116058277A (en) * | 2022-09-08 | 2023-05-05 | 广西南亚热带农业科学研究所 | Tea tree hybridization marking method and early seedling breeding method of hybrid seeds |
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| CN1843089A (en) * | 2006-05-15 | 2006-10-11 | 西北农林科技大学 | Self-crossing pollination method for maize artificial enveloping |
| CN107333647A (en) * | 2017-09-08 | 2017-11-10 | 湛江市农业科学研究院（广东省（湛江）区域性农业试验中心） | A kind of labeling method of plant hybridization breeding combination |
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2018
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| CN1843089A (en) * | 2006-05-15 | 2006-10-11 | 西北农林科技大学 | Self-crossing pollination method for maize artificial enveloping |
| CN107333647A (en) * | 2017-09-08 | 2017-11-10 | 湛江市农业科学研究院（广东省（湛江）区域性农业试验中心） | A kind of labeling method of plant hybridization breeding combination |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112136684A (en) * | 2020-09-21 | 2020-12-29 | 连云港市农业科学院 | Efficient testing and matching method for corn line selection |
| CN116058277A (en) * | 2022-09-08 | 2023-05-05 | 广西南亚热带农业科学研究所 | Tea tree hybridization marking method and early seedling breeding method of hybrid seeds |
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Application publication date: 20190108 |