CN108184654A - A kind of preparation method of Maize hybridized combination - Google Patents
A kind of preparation method of Maize hybridized combination Download PDFInfo
- Publication number
- CN108184654A CN108184654A CN201810130872.8A CN201810130872A CN108184654A CN 108184654 A CN108184654 A CN 108184654A CN 201810130872 A CN201810130872 A CN 201810130872A CN 108184654 A CN108184654 A CN 108184654A
- Authority
- CN
- China
- Prior art keywords
- maternal
- preparation
- male parent
- combination
- parent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Abstract
The invention discloses a kind of preparation methods of Maize hybridized combination, are selfed stability series by selection and determine maternal and male parent, it would be desirable to which a variety of maternal and a kind of male parents being measured are in field according to 4:1 row is than composition isolated area, and different isolated areas is set as according to the type difference of male parent, security partition distance is equipped between different isolated areas, including space separation distance or time-division distance, and the female parent manually pumps tassel, female fringe is without bagging isolation, the open paternal pollen repeatedly received by anemophily in this isolated area, pollination and setting percentage are improved, also is adapted for the preparation of large area Maize hybridized combination.
Description
Technical field
The invention belongs to agricultural technology field, more particularly to a kind of preparation method of Maize hybridized combination.
Background technology
The preparation method of traditional Maize hybridized combination, by taking the Shandong Province of Huang-Huai-Hai as an example, sowing at the beginning of usually 6 months
Corn breeding material, will be selfed choosing is 4 thousands of parts of progress field plantings of selfing based material more than generation basicly stable, is grown according to row
Plantation row is arranged between 5 meters of different materials, setting interval passageway prevents from mixing between row and row.Corn inbred line grows to the florescence
Before, it needs manually in advance to set up the female fringe of corn using the sulfuric acid paper bag of waterproof so that the flower that future, female fringe grew out
Silk does not reach pollen, can only receive the paternal pollen that breeder specifies by way of artificial pollination, beautiful so as to complete to prepare
The hybrid process of rice Combination nova.This method for preparing corn new cross combination needs to put into a large amount of man power and material, and suitable
The high temperature and humidity weather at the beginning of August is met, corn breeding personnel drip with sweat in field shuttle powder collecting, pollinate, or so front and rear half a month
Time could complete the preparation work of a large amount of Combination novas.
Invention content
To solve the above problems, the present invention provides a kind of preparation method of Maize hybridized combination, the preparation method is significantly
Man power and material is saved, avoids the operation under the conditions of high temperature and heating summer, while also improves the success rate of corn hybridization.
Based on this, a kind of preparation method of Maize hybridized combination provided by the invention includes the following steps：
A. selection is selfed stability series and is measured cross combination；
B. more selfing stability series are chosen as female parent and sow congener female parent in a row, the maternal sowing is more
Row, and maternal type of not going together is different and sows simultaneously；
C. it chooses and the maternal male parent being measured, the male parent parallel rows is simultaneously sowed simultaneously；
D. multiple types female parent needs being measured is an isolated area in field planting with a kind of male parent, according to male parent type
Difference be set as different isolated areas；
E. it is provided with security partition distance between different isolated areas or between other corn fields；
F. maternal tassel is manually pumped, retain maternal female fringe and is not required to bagging isolation；
G. after maternal female fringe filigree discharge, receive the pollen of male parent tassel by anemophily, complete hybridization pollination, obtain new
Cross combination seed.
Further, in step a, the selfing stability series are 4 stability series more than generation of selfing.
Further, in step b, the line number of the diverse maternal sowing simultaneously is 4 rows.
Further, in step e, the security partition distance is space separation distance or time-division distance, and described
Space separation distance is not less than 300 meters, the time-division distance no less than 20 days.
Implement the embodiment of the present invention, have the advantages that：
1st, in the present invention Maize hybridized combination preparation method, by the way that a kind of a variety of maternal and male parents are formed an isolation
Area is set as different isolated areas, and security partition distance is equipped between different isolated areas according to male parent type difference, realizes
Economical and efficient theory；It is maternal simultaneously it is open paternal pollen is repeatedly received by anemophily, and without bagging isolation, greatly
Man power and material is saved, cost-effective and breeder time, improves pollination and setting percentage.
2nd, in the present invention, the line number for receiving different types of female parent of same male parent is set as 4 rows, it is ensured that maternal
Female fringe filigree is uniformly pollinated, and is saved man power and material, also is adapted for the preparation of the corn Combination nova of large area.
3rd, in the present invention, security partition distance is provided between different isolated areas or between other corn fields, and
Security partition distance is space separation distance or time-division distance, and the space separation distance is not less than 300 meters, the time
Separation distance is no less than 20 days, eliminates the interference of the foreign pollen except this isolated area.
Description of the drawings
Fig. 1 is the selection and breeding flow chart of the Maize hybridized combination preparation method described in the present embodiment.
Fig. 2 is the field layout drawing one of the Maize hybridized combination preparation method described in the present embodiment.
Fig. 3 is the field layout drawing two of the Maize hybridized combination preparation method described in the present embodiment.
Reference sign：
Wherein, 1 it is, maternal, 2, male parent, the 21, first male parent, the 22, second male parent, 3, security partition distance, 4, marker space,
41st, the first marker space, the 42, second marker space.
Specific embodiment
Below in conjunction with the drawings and examples in the present invention, technical scheme of the present invention is clearly and completely retouched
It states, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.Based on the present invention
In embodiment, the every other implementation that those of ordinary skill in the art are obtained without making creative work
Example, belongs to protection scope of the present invention.
With reference to shown in Fig. 1 to Fig. 3, the preparation method of a kind of Maize hybridized combination that the embodiment of the present invention is provided, including
Following steps：
A. selection is selfed stability series and is measured cross combination；
B. more selfing stability series are chosen as female parent 1 and are sowed congener maternal 1 in a row, 1 sowing of female parent is more
Row, and the type for female parent 1 of not going together is different and sow simultaneously；
C. it chooses and maternal 1 male parent 2 being measured, 2 parallel rows of male parent is simultaneously sowed simultaneously；
D. the multiple types female parent 1 needs being measured is an isolated area 4 in field planting with a kind of male parent 2, according to male parent 2
The difference of type is set as different isolated areas 4；
E. it is provided with security partition distance 3 between different isolated areas 4 or between other corn fields；
F. maternal 1 tassel is manually pumped, retain maternal 1 female fringe and is not required to bagging isolation；
G. after maternal 1 female fringe filigree spues, receive the pollen of 2 tassel of male parent by anemophily, complete hybridization pollination, obtain
New cross combination seed.
The preparation method of Maize hybridized combination in the embodiment of the present invention, by the way that a variety of maternal 1 and a kind of male parent 2 are formed one
A isolated area 4 is set as different isolated areas 4, and security partition is equipped between different isolated areas 4 according to 2 type difference of male parent
Distance 3 realizes economical and efficient theory；Female parent 1 is open simultaneously repeatedly receives 2 pollen of male parent by anemophily, and need not
Bagging isolation is greatly saved man power and material, cost-effective and breeder time, improves pollination and setting percentage.
Wherein, it is 4 stability series more than generation of selfing that stability series are selfed described in above-mentioned steps a, wherein choosing more
It is congener selfing stability series as female parent 1 side by side in a row when, preferably 5 it is congener selfing stability series 1 conducts of female parent
A line.In above-mentioned steps b, the line number of diverse maternal 1 sowing simultaneously is 4 rows, can both realize that a line male parent 2 corresponded to
Multirow female parent 1 avoids the waste of pollen resource, it is also ensured that the female fringe on female parent 1 can all receive the flower of male parent 2
Powder, so as to complete pollinating process.
Specific embodiment one：
With reference to shown in Fig. 2, male parent 2 is one species, and the present embodiment belongs to same isolated area 4, needs and other corns
Security partition distance 3 between field is set, but is not required to the setting security partition distance 3 in this isolated area 4, so as to realize a line
The interval of male parent 2 and multirow female parent 1 is sowed, while sowing is also convenient for later stage harvest, and the corn Combination nova of suitable large area is matched
System.
Specific embodiment two：
With reference to shown in Fig. 3, different isolated areas 4 according to the type difference of male parent 2 is set, is set between different isolated areas 4
Security partition distance 3 is equipped with, male parent 2 includes the first male parent 21 and the second male parent 22 in the present embodiment, and isolated area 4 includes first
41 and second isolated area 42 of isolated area, the first male parent 21 and female parent 1 form 1 group of the first isolated area 41, the second male parent 22 and female parent
Into the second isolated area 42；Wherein the space separation distance of the first isolated area 41 and the second isolated area 42 is not less than 300 meters, the time point
Gauge eliminated the pollination interference of different isolated areas 4 from no less than 20 days, and what maternal 1 female fringe can be open repeatedly receives father
This 2 pollen anemophily so as to improve the pollination of female parent 1 and setting percentage, also is adapted for the preparation of large area corn hybridization Combination nova,
Save man power and material.
Based on this, practical application is carried out for the preparation method of the Maize hybridized combination, to traditional technology and the invention
The embodiment described is compared.For preparing 5000 corn Combination novas in the conventional way, the workload probably needed is calculated,
Each Combination nova, which generally requires, prepares 3 fringes, and 5000 Combination novas need number of productive ear 15000, according to the effective fringe in 80% field
Number ratio, it is necessary to which 18750 tassels, generally growth normal plant spike number is 4000 or so per acre, it is necessary to land area
4.68 mu.
1st, in traditional technology, the hybridization pollination meter of 220 Combination novas is prepared daily according to one group of 2 people of worker that is skilled in technique
It calculates, needs 23 people altogether.
2nd, the preparation method of the Maize hybridized combination of the invention takes out everyone male 3 mu of calculating in one day according to artificial, excludes and pass
The identical sowing of system method, harvest recruitment, new method, which prepares 5000 corn new cross combinations, needs 1.56 manually；When
21.44 have been saved compared to conventional method manually, while also eliminate the high temperature and heating summer of conventional approach August part artificial pollination.Two
It is to save a large amount of bagging expense, during the preparation Maize hybridized combination of the invention, the female fringe of female parent 1 does not need to bagging isolation, matches
5000 Combination novas of system save 18750 sulfuric acid paper bags, according to each 0.11 yuan of calculating of sulfuric acid paper bag, save expense 2062.5
Member；According to 1200 calculating of the daily bagging of the worker that is skilled in technique, new method saves artificial 15.6 manually, according to current people
Expenses of labour 100 yuan of calculating for each person every day, saves 1560 yuan of bagging expense.
In short, artificial and bagging cost two is total, the preparation method of the Maize hybridized combination saves altogether
3622.5 yuan, especially for large-scale seeds company, the new cross combination number that their required corn breedings are prepared is general
Reach 2~more than 30,000, the quantity of the new cross combination seed of configuration is more, and the advantage which is showed can be bigger.
To sum up, the preparation method of the Maize hybridized combination described in the embodiment of the present invention, by by a variety of maternal 1 and a kind of father
This 2 composition, one isolated area 4, different isolated areas 4 are set as, and set between different isolated areas 4 according to the type difference of male parent 2
There is security partition distance 3, realize the theory of economical and efficient, while be also convenient for the later stage by anemophily propagation to receive male parent 2
Pollen, and without bagging isolation, it is greatly saved man power and material, cost-effective and breeder time, so as to succeed
Into pollination, pollination and setting percentage are improved.
The above is only the preferred embodiment of the present invention, it should be pointed out that for those skilled in the art
For, without departing from the technical principles of the invention, several improvement and replacement can also be made, these improve and replace
It should be regarded as protection scope of the present invention.
Claims (4)
1. a kind of preparation method of Maize hybridized combination, which is characterized in that include the following steps：
A. selection is selfed stability series and is measured cross combination；
B. more selfing stability series are chosen as female parent and sow congener female parent in a row, the maternal sowing multirow,
And maternal type of not going together is different and sows simultaneously；
C. it chooses and the maternal male parent being measured, the male parent parallel rows is simultaneously sowed simultaneously；
D. multiple types female parent needs being measured is an isolated area in field planting with a kind of male parent, according to male parent type not
It is same to be set as different isolated areas；
E. it is provided with security partition distance between different isolated areas or between other corn fields；
F. maternal tassel is manually pumped, retain maternal female fringe and is not required to bagging isolation；
G. after maternal female fringe filigree discharge, receive the pollen of male parent tassel by anemophily, complete hybridization pollination, newly hybridized
Combine seed.
2. the preparation method of Maize hybridized combination as described in claim 1, which is characterized in that in step a, the selfing is stablized
It is to be selfed 4 stability series more than generation.
3. the preparation method of Maize hybridized combination as described in claim 1, which is characterized in that in step b, the type is different
The line number of maternal sowing simultaneously be 4 rows.
4. the preparation method of Maize hybridized combination as described in claim 1, which is characterized in that in step e, the security partition
Distance is space separation distance or time-division distance, and the space separation distance is not less than 300 meters, the time-division away from
From no less than 20 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810130872.8A CN108184654A (en) | 2018-02-09 | 2018-02-09 | A kind of preparation method of Maize hybridized combination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810130872.8A CN108184654A (en) | 2018-02-09 | 2018-02-09 | A kind of preparation method of Maize hybridized combination |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108184654A true CN108184654A (en) | 2018-06-22 |
Family
ID=62593446
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810130872.8A Pending CN108184654A (en) | 2018-02-09 | 2018-02-09 | A kind of preparation method of Maize hybridized combination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108184654A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197578A (en) * | 2018-11-22 | 2019-01-15 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of selection of P crowds of corn inbred line Lx286 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1692711A (en) * | 2005-05-16 | 2005-11-09 | 山东登海种业股份有限公司 | Method for cross breeding of corn |
| CN101081003A (en) * | 2007-06-25 | 2007-12-05 | 北京金色农华种业科技有限公司 | Corn hybrid breeding method |
| CN103733981A (en) * | 2014-01-15 | 2014-04-23 | 新疆农垦科学院 | Hybrid wheat seed production method by facility isolation |
| CN106332774A (en) * | 2016-11-02 | 2017-01-18 | 陕西省杂交油菜研究中心 | Efficient breeding method adopting multi-female-parent one-male-parent mode to prepare oilseed rape hybrid |
| CN106359074A (en) * | 2016-08-24 | 2017-02-01 | 张掖市农业科学研究院 | One-male-in-two-female corn seed production method |
-
2018
- 2018-02-09 CN CN201810130872.8A patent/CN108184654A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1692711A (en) * | 2005-05-16 | 2005-11-09 | 山东登海种业股份有限公司 | Method for cross breeding of corn |
| CN101081003A (en) * | 2007-06-25 | 2007-12-05 | 北京金色农华种业科技有限公司 | Corn hybrid breeding method |
| CN103733981A (en) * | 2014-01-15 | 2014-04-23 | 新疆农垦科学院 | Hybrid wheat seed production method by facility isolation |
| CN106359074A (en) * | 2016-08-24 | 2017-02-01 | 张掖市农业科学研究院 | One-male-in-two-female corn seed production method |
| CN106332774A (en) * | 2016-11-02 | 2017-01-18 | 陕西省杂交油菜研究中心 | Efficient breeding method adopting multi-female-parent one-male-parent mode to prepare oilseed rape hybrid |
Non-Patent Citations (2)
| Title |
|---|
| 宋邦钧等: "《作物遗传与育种学 第3分册 作物育种与良种繁育各论（北方本）》", 31 October 1988, 农业出版社 * |
| 张春庆等: "《玉米水稻杂交种子生产技术》", 31 March 2015, 山东科学技术出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109197578A (en) * | 2018-11-22 | 2019-01-15 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | A kind of selection of P crowds of corn inbred line Lx286 |
| CN109197578B (en) * | 2018-11-22 | 2021-11-05 | 山东省农业科学院玉米研究所（山东省农业科学院玉米工程技术研究中心） | Breeding method of P-group maize inbred line Lx286 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100493341C (en) | Method for selecting wheat of new variety | |
| CN103355159B (en) | A kind of selection of perennial forage maize | |
| CN104472157B (en) | A kind of breeding method of Abelmoschus esculentus new varieties | |
| CN106665332A (en) | Method for carrying out recurrent selection breeding on water-saving and drought-resisting rice by utilizing dominant nuclear male sterile material | |
| CN109122300A (en) | A kind of tea tree hybridization breeding method | |
| CN102239800A (en) | Breeding method for triploid cassava | |
| CN103461101B (en) | Stemless cucurbita maxima breeding method | |
| CN103299896A (en) | Culturing method of eurytropic bolting-resisting spring Chinese cabbage free microspores | |
| CN105379615A (en) | Wheat blue and abortive sterile germplasm and breeding method thereof | |
| CN107896977B (en) | Method for cultivating rape comprehensive hybrid | |
| CN104303731A (en) | Zucchini breed hybrid seed production method | |
| CN101124886B (en) | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration | |
| CN108184654A (en) | A kind of preparation method of Maize hybridized combination | |
| CN105075855A (en) | Method for breeding male sterility restoring line of yellow sprout pepper cytoplasm | |
| CN104719130A (en) | Method for increasing seed production yield of hybrid corns by virtue of talcum powder | |
| CN103190336A (en) | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids | |
| CN108633660B (en) | A kind of Radix Salviae Miltiorrhizae new varieties production of hybrid seeds method for culturing seedlings | |
| CN104170725B (en) | The selection of a kind of high yield, disease-resistant collard | |
| CN102668975A (en) | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker | |
| CN106359074A (en) | One-male-in-two-female corn seed production method | |
| CN105145336A (en) | Breeding method for sunflower hybrid seed AR7-7376 with white outer shell | |
| CN110338053B (en) | Breeding method of orange pagoda cauliflower hybrid | |
| CN107637514B (en) | Mechanized high-efficiency seed production and sowing method for hybrid wheat | |
| CN108055989B (en) | High-yield planting method for interplanting spinach and soybeans | |
| CN101606484A (en) | A kind of method of utilizing colored cotton specialized character seed selection hybrid cotton |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180622 |