CN101081003A - Corn hybrid breeding method - Google Patents

Corn hybrid breeding method Download PDF

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Publication number
CN101081003A
CN101081003A CN 200710117811 CN200710117811A CN101081003A CN 101081003 A CN101081003 A CN 101081003A CN 200710117811 CN200710117811 CN 200710117811 CN 200710117811 A CN200710117811 A CN 200710117811A CN 101081003 A CN101081003 A CN 101081003A
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male parent
hybrid
parent
phase
corn
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蔡士斌
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Beijing Jinse Nonghua Seed Science & Technology Co Ltd
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Beijing Jinse Nonghua Seed Science & Technology Co Ltd
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Abstract

The present invention belongs to the field of heterosis utilizing technology, and is especially process of breeding corn hybrid. The selfing line CH584 as female parent and selfing line CH361 as male parent are hybridized to breed corn hybrid Nonghua-9. The selfing line CH584 is obtained through breeding third copulation hybrid with (7922x8112) and LD143, and continuous eight generation of selfing breeding by means of pedigree method. The selfing line CH361 is obtained through breeding hybrid species with Dan-599 and Dan-340, natural opened F1 selection to obtain excellent single plant, and and continuous eight generation of selfing breeding by means of pedigree method. The corn hybrid has the features of high and stable yield, high disease resistance, high biological yield, etc.

Description

A kind of method of hybrid maize seed production
Technical field
The invention belongs to the heterosis utilization field, specifically the present invention is a kind of method of hybrid maize seed production.
Background technology
Corn is a heterosis utilization crop the earliest in the world, and since the thirties, countries in the world are all utilizing the hybrid vigour seed selection and promoting the superior corn new hybrid, as an important technology approach that improves corn yield, improvement corn quality from eighties of last century.The U.S. popularized corn hybrid seed in 1956, and the sowing area of China's corn hybrid seed in 1987 accounts for 80% of corn seeding area.Selecting and breeding corn crossbreed major technique link comprises according to breeding objective and carries out inbred line breeding, parental apolegamy, the evaluation selection of the new combination of hybridization, the regional test evaluation of different ecological environment etc.Corn breeding and produce advanced country such as the U.S., France, Germany is in the world produced and the continuous demand in market in order to satisfy, in time seed selection and release generation by generation new varieties and change original kind.
Since the eighties, China introduces large quantities of crossbreed from American-European corn main product state and is used for test, research.To at present, the research of the corn breeding of China mainly is based on the seed selection single cross hybrid.In four kind renewal processes of having carried out, the 4th generation crossbreed promoted ten aborning surplus year, the purity of parental inbred line descends, the resistance decline, therefore the output of crossbreed, regularity have been much worse than at the beginning of the popularization, and selecting coordinate force height, economical character is good, genotype is isozygotied inbred line preparing hybrid kind for use is to improve the key of corn yield.Peasant user expects the new varieties of high yield, high-quality, better resistance.
Summary of the invention
The objective of the invention is to utilize the hybrid vigour theory, select high-combining ability, economical character is good, genotype is isozygotied, parents' affiliation is far away hybridization between selfed lines for use, select high yield, disease-resistant corn hybrid seed.
The invention provides a kind of method of hybrid maize seed production, comprise the seed selection inbred line, safety is isolated, and regulates date of seeding, suitable row ratio, roguing, the maternal castration rooted out male parent, results utilize inbred line CH584 to be female parent in good time, and inbred line CH361 is a paternal hybrid, the preparation corn hybrid seed.
Wherein said inbred line CH584 combines triple hybrid with (7922 * 8112) and LD143 hybridization, adopts pedigree method, through continuous 8 generation selfed breeding form.
Wherein said inbred line CH361 be with red 599 with the crossbreed of red 340 hybridization assembly, F1 adopts pedigree method for selecting open waterlogging-resistant, the disease-resistant fine individual plant of nature, through continuous 8 generation selfed breeding form.
The biomaterial 7922,8112 that the present invention relates to, LD143, red 599 and red 340 are all open in non-patent literature, and above-mentioned biomaterial all has preservation at the applicant place, and the applicant guarantees to provide to the public in 20 years present patent application days.
Wherein 7922 at Wang Kuisen, Wang Sheng, and Wang Xiuhua, etc. the seed selection of maize elite inbred line 7922 and application [J]. external agronomy--coarse cereals crop, 1995, (1): open in 50~51..
8112 at Wu De phase .8112 corn inbred line [J]. seed science and technology, and 1993, open in (5).
" new variety of agricultural plant power protection communique " the 4th phase in 2006 that LD143 has published on July 1st, 2006, in the 35th page record its be applied in the breeding as parent material.
Red 599 " new variety of agricultural plant power protection communique " the 3rd phases in 2003 of having published on May 1st, 2003, in the 16th page record its be applied in the breeding as parent material.
Red 340 at Wu Yuchun. and hybridization maize is newly made up not 17Ht * red 340 high-yield culture technique pre-tests. farming and cultivation, 1988, open in (3).
The present invention utilizes inbred line CH584 to be female parent, and inbred line CH361 is the feature of the corn hybrid seed farming China 9 of paternal hybrid preparation: seedling leaf sheath purple, leaf green, leaf margin aubergine; The plant type compactness, 315 centimetres of plant heights, 137 centimetres of fringe positions become strain number of blade 21-22 sheet; Tassel branch 15-19, clever shell green, flower pesticide yellow, the filigree green, fruit ear cartridge type, 23.8 centimetres of spike lengths, bald sharp 3.3 centimetres, tassel row number 16-20 is capable, the seed yellow, semihard grain type, on the rice matter, 100-grain weight 39.2 grams, cob redness, the seed rate of output 85.9%, moisture content of kernels is 21.8% during threshing; Sow in spring breeding time 134 days (Liaoning Area), need more than 10 ℃ 2900 ℃ of active accumulated temperatures,, belong to late-maturing crossbreed than red beautiful 39 Zao 1 day.
The feature of parental inbred line CH584: the seedling leaves green, the leaf sheath purple, early onset is good; The plant type compactness, 173 centimetres of plant heights, 73 centimetres of fringe positions, thick 2.5 centimetres of stem, complete stool number of blade 20-21 sheet; Tassel branch 3-6, clever shell green, the flower pesticide yellow, the filigree green, the long cartridge type of fruit ear, 21.5 centimetres of spike lengths, thick 4.6 centimetres of fringe, tassel row number 14-16 is capable, seed yellow, half horse flute profile, 100-grain weight 33.0 grams, cob redness; Sow in spring breeding time in 128 days (Shenyang); The Chinese People's Anti-Japanese Military and Political College, helminthosporium maydis, anti-head-smut, anti-stem rot, resistant to lodging.
The feature of parental inbred line CH361: the seedling leaves green, the leaf sheath purple, early onset is good; The plant type compactness, 201 centimetres of plant heights, 76 centimetres of fringe positions, thick 2.7 centimetres of stem, complete stool number of blade 21-22 sheet; Tassel branch 11-16, clever shell green, the flower pesticide yellow, the filigree green, the fruit ear cartridge type, 22.5 centimetres of spike lengths, thick 5.9 centimetres of fringe, tassel row number 18-22 is capable, the seed yellow, half horse flute profile, 100-grain weight 39.8 grams, cob white, single fringe grain weighs 148.3 grams; Sow in spring breeding time in 132 days (Shenyang); The Chinese People's Anti-Japanese Military and Political College, helminthosporium maydis, anti-head-smut, anti-stem rot, resistant to lodging, resistance to overhead flooding injury is strong.
Advantage of the present invention and good effect are:
1. output height, output surpasses main breed 5% in the current production;
2. best in quality, reach the GB high-quality index;
3. resistance, disease-resistanting leaf spot, stem rot, head smut; Resistant to lodging, the drought resisting resistance to overhead flooding injury is strong;
4. wide adaptability.
Description of drawings
Fig. 1 is a hybrid seeding family tree of the present invention.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1: the concrete seed selection process of crossbreed farming magnificent 9 of the present invention
Combined triple hybrid with 7922 * 8112 with LD143 hybridization in 1998, and adopted pedigree method, breed inbred line CH584 through continuous 8 generations selfing.
Crossbreed with pellet 599 and red 340 hybridization assembly in 1994, F 1In generation, selected open waterlogging-resistant, the disease-resistant fine individual plant of nature, adopts pedigree method, breeds inbred line CH361 through continuous 8 generations selfing.
It is female parent with inbred line CH584 that winter in 2002 was bred the base in cities and towns, precipice, Sanya, Hainan Province, and CH361 forms corn single cross hybrid farming China 9 for the paternal hybrid assembly.Crossbreed farming magnificent 9 of the present invention was participated in product than test in 2003; Participated in late-maturing group of preliminary experiment of the new combination of Liaoning Province's corn in 2004; 2005-2006 participates in late-maturing group of regional test of the new combination of Liaoning Province's corn; Participated in late-maturing group of production test of the new combination of Liaoning Province's corn in 2006 simultaneously.In the tests in 3 years, demonstration, farming China 9 shows that high yield, high-quality, fringe are big, and yielding ability is outstanding, and comprehensive resistance is strong, characteristics such as wide adaptability; Than red beautiful No. 39 average volume increase 11.7-12.2%.
1. quality is identified
2005 through agricultural product quality supervision and inspection center of the Ministry of Agriculture (Shenyang) assay: seed contains crude protein 10.76%, crude fat 5.40%, thick starch 71.36%, lysine 0.34%, unit weight 771.1 grams (seeing Table 1).
Magnificent 9 attributional analysis assay unit: the % of table 1. farming, G/L.
The unit of being examined Sample number into spectrum Crude protein Crude fat Thick starch Lysine Unit weight
Tieling Academy of Agricultural Sciences №o2006-1-1220 10.76 5.40 71.36 0.34 771.1
2. stable
Provinces and regions, Liaoning in 2005 examination summarized results: standard deviation 117.51, the coefficient of variation 20.27, stability parameter 1.13; Provinces and regions, Liaoning in 2006 examination summarized results: standard deviation 65.48, the coefficient of variation 8.94, stability parameter 0.80; Liaoning Province's production test summarized results standard deviation 81.43 in 2006, the coefficient of variation 11.93, stability parameter 1.09.
3. resistance
Late-maturing group of regional test field investigation summarized results of the new combination of 2005-2006 Liaoning Province corn: leaf blight 1-5 level, head smut diseased plant rate 0-8.5%, stem rot 0-5 level, graywall 1-3 level, Curvularia lunata leaf spot 1-5 level, lodging rate 0-1.3%, the rate of falling folding 0-3.3%.The disease-resistant worm of plant protection institute of 2005-2006 Agricultural University Of Shenyang is identified summarized results: leaf blight 1-5 level, graywall 1-3 level, Curvularia lunata leaf spot 1-5 level, stem rot 1-5 level, head smut (diseased plant rate %) 0.9-8.5.Prove through the inoculation qualification result: anti-(R-MR) leaf blight in farming China 9, anti-(R) graywall, in anti-(R-MR) Curvularia lunata leaf spot, in anti-(R-MR) stem rot, in anti-(HR-MR) head smut (seeing Table 2).
The 9 disease-resistant inoculations of table 2.2005-2006 farming China are identified tabulating unit: %, level
The time limit Leaf blight (sick level) Graywall (sick level) Curvularia lunata leaf spot (sick level) Stem rot (sick level) Silk dust-brand (diseased plant rate %)
2005 3-5 1-3 1-5 5 0-0.9
2006 1-3 1-3 3 1-3 0-8.5
4. output performance
1) produces than the test result
2003 produce per year than 693.3 kilograms of test per mu yields, and comparison is according to agricultural university's 108 volume increase 14.2%.
2) Liaoning Province's preliminary experiment
Economized 785.0 kilograms of 7 average yield per mus of preliminary experiment in 2004, comparison on average increases production 5.6% according to 743.6 kilograms of red beautiful 39 per mu yields, and output occupies the 33rd (seeing Table 3).
Table 3.2004 year Liaoning Province's preliminary experiment farming magnificent 9 yield result units: KG
Hold examination unit Per mu yield Than CK ± % Precedence
Liaoning Province Academy of Agricultural Sciences 929.1 20.1 6
Tieling Academy of Agricultural Sciences 825.0 1.9 81
The young agricultural academy of sciences 783.5 -3.4 137
Dalian Academy of Agricultural Sciences 636.9 0.3 119
Dandong Academy of Agricultural Sciences 514.6 -12.4 194
Haicheng City Academy of Agricultural Sciences 920.2 18.7 4
Jinzhou Academy of Agricultural Sciences 885.4 8.8 64
The whole province is average 785.0 5.6 33
3) Liaoning Province's regional test
579.8 kilograms of 9 average yield per mus of provinces and regions examination in 2005, comparison are on average increased production 17.3%, 9 full volume increase according to 494.2 kilograms of red beautiful 39 per mu yields, amount of increase in production 5.6-31.7%, and output occupies D and organizes the 2nd.
732.4 kilograms of 9 average yield per mus of provinces and regions examination in 2006, comparison are on average increased production 8.5%, 9 full volume increase according to 675.2 kilograms of red beautiful 39 per mu yields, amount of increase in production 0.9-28.0%, and output occupies B and organizes the 6th.
656.1 kilograms of 2005-2006 provinces and regions examination average yield per mus, comparison is according to red beautiful 39 average volume increase 12.2%, and output occupies the 8th (seeing Table 4).
The magnificent 9 provinces and regions test manufacture amounts of table 4.2005-2006 farming are tabulating unit: KG as a result
Hold examination unit 2005 2006
Per mu yield Than CK ± % The contrast per mu yield Precedence Per mu yield Than CK ± % The contrast per mu yield Precedence
Dandong Academy of Agricultural Sciences 396.3 13.2 350.1 12 647.3 4.8 617.4 7
Tieling Academy of Agricultural Sciences 637.9 16.6 547.0 2 796.0 2.1 779.6 5
Corn research institute of Liaoning Province Academy of Agricultural Sciences 661.8 23.5 536.0 5 730.4 1.5 719.2 9
Kaiyuan research of agricultural science institute 675.7 30.0 519.7 2
Jinzhou Academy of Agricultural Sciences 561.7 5.6 532.2 11 777.2 28.0 607.0 4
Agrotechnical center, Lingyuan City 706.4 31.7 536.4 7
Sea standing grain kind industry 648.8 15.9 559.9 5 757.0 7.5 704.0 6
Wide pasture Academy of Agricultural Sciences 3943 8.0 365.0 11
The East Asia seed academy of sciences 535.7 6.8 501.4 3
The Chaoyang City seed control station 824.1 4.5 789.0 4
Development zone, Dalian seed stations 625.2 6.6 586.6 12
Agrotechnical center, Zhuanghe City 714.5 27.4 561.0 3
East Asia (Changtu) Academy of Agricultural Sciences 719.8 0.9 713.4 13
The whole province is average 579.8 17.3 494.2 2 732.4 8.5 675.2 6
4) Liaoning Province's production test
Economized 682.5 kilograms of 7 average yield per mus of production test in 2006, comparison is on average increased production 11.7%, 7 full volume increase according to 611.0 kilograms of red beautiful 39 per mu yields, amount of increase in production 5.9-15.7%, and output occupies the 2nd (seeing Table 5).
Table 5.2005 year magnificent 9 province's production test yield result tabulating unit: the KG of farming
Hold examination unit Per mu yield Than CK ± % The contrast per mu yield Precedence
Dandong Academy of Agricultural Sciences 552.7 11.4 496.3 5
Tieling Academy of Agricultural Sciences 735.4 15.7 635.5 1
Shenyang Academy of Agricultural Sciences 738.6 5.9 697.3 3
Jinzhou Academy of Agricultural Sciences 736.6 16.2 633.8 3
Haicheng City Academy of Agricultural Sciences 742.6 11.1 668.4 1
Dalian Academy of Agricultural Sciences 690.7 13.8 607.0 2
Changtu Academy of Agricultural Sciences 580.5 7.7 538.9 2
The whole province is average 682.5 11.7 611.0 2
Embodiment 2: the seeding technique of crossbreed farming magnificent 9 of the present invention
1. safety is isolated:
Distance isolation: require not plant more than 300 meters other corn around the production of hybrid seeds district.
Time isolates: the sowing time production of hybrid seeds district in will with other corn mistake phase more than 35 days.
2. it is maternal that adjusting date of seeding: CH584 does, and CH361 makes male parent, broadcast the first phase during male parent earlier male parent seed soaking in three days in advance, sow simultaneously with female parent, broadcast second phase male parent again after first phase male parent is emerged.
3. suitable row ratio: father, maternal going than adopting 1: 5,1 row male parent is planted at the maternal interval of promptly per 5 row, and female parent is 4500 strain/mus, male parent 2800 strain/mus.
4. strict roguing:
Be to guarantee purity of hybrid, should be in seedling stage, shooting stage, take out and will not meet the assorted strain of parent's characteristic feature characteristic, different strain, lopsided strain, diseased plant and weak strain male early stage and all pull out.
5. maternal the castration rooted out male parent.
The maternal castration is the key link of production of hybrid seeds work, is the necessary means that obtains pure hybrid seed, must employing take off luxuriant emasculating method in advance, accomplishes thorough, clean.After the female parent pollination in production of hybrid seeds district finishes, male parent is all rooted out, help ventilation and penetrating light, improve output, prevent to mix, ensure the quality of products.
6. gather in the crops the airing keeping in good time.
In time gather in the crops after the production of hybrid seeds district corn maturation, avoid meeting rain fruit ear germination or mildew and rot, after threshing was dried, the registration warehouse-in was taken care of.
7. diseases prevention worm.
Plant clothing agent (farming (Hai Li) Zhuozhou, north kind clothing agent Co., Ltd) with 16% good fortune gram azoles alcohol (black Mi Jing) and carry out Cotton seeds, the typhon mouth phase can add 0.7% urea (east, Chengdu aurification reagent Co., Ltd) control of maize snout moth's larva and male parent row aphid with 0.4% potassium dihydrogen phosphate.
Embodiment 3: the seeding technique of crossbreed farming magnificent 9 of the present invention
1. safety is isolated:
Distance isolation: require not plant more than 300 meters other corn around the production of hybrid seeds district.
Time isolates: the sowing time production of hybrid seeds district in will with other corn mistake phase more than 35 days.
2. adjusting date of seeding: CH584 does maternal, and CH361 makes male parent, broadcasts first phase male parent earlier, when the first phase, male parent went out root, broadcasts female parent, after first phase male parent is emerged, broadcasts second phase male parent again.
3. suitable row ratio: father, maternal going than adopting 1: 5,1 row male parent is planted at the maternal interval of promptly per 5 row, and female parent is 4500 strain/mus, male parent 3000 strain/mus.
4. strict roguing:
Be to guarantee purity of hybrid, should be in seedling stage, shooting stage, take out and will not meet the assorted strain of parent's characteristic feature characteristic, different strain, lopsided strain, diseased plant and weak strain male early stage and all pull out.
5. maternal the castration rooted out male parent.
The maternal castration is the key link of production of hybrid seeds work, is the necessary means that obtains pure hybrid seed, must employing take off luxuriant emasculating method in advance, accomplishes thorough, clean.After the female parent pollination in production of hybrid seeds district finishes, male parent is all rooted out, help ventilation and penetrating light, improve output, prevent to mix, ensure the quality of products.
6. gather in the crops the airing keeping in good time.
In time gather in the crops after the production of hybrid seeds district corn maturation, avoid meeting rain fruit ear germination or mildew and rot, after threshing was dried, the registration warehouse-in was taken care of.
7. diseases prevention worm.
Plant clothing agent (farming (Hai Li) Zhuozhou, north kind clothing agent Co., Ltd) with 16% good fortune gram azoles alcohol (black Mi Jing) and carry out Cotton seeds, the typhon mouth phase can add 0.7% urea (east, Chengdu aurification reagent Co., Ltd) control of maize snout moth's larva and male parent row aphid with 0.4% potassium dihydrogen phosphate.
Embodiment 4: the culture technique of crossbreed farming magnificent 9 of the present invention
Suit to plant in the late-maturing corn district more than 2900 ℃ at effective accumulated temperature such as Shenyang, Liaoning, Dalian, Anshan, Jinzhou, Tieling, Chaoyang, Dandong.
1. date of seeding and seed treatment
Choosing ground and date of seeding: the level land of suitable medium fertility, low-lying land plantation; The 5 centimetres of ground temperature in the face of land are stable to be suitable seedtime by 8 ℃.
Seed treatment: handle with kind of clothing agent a carrying out seed pelleting, improves pest-resistant, resistance against diseases, guarantee once to sow and reach that seedling is neat, seedling is complete, seedling is strong.
2. planting density and form
Suitable clear the kind, planting density 3200 strain/mus, best results strain number is 3000 strain/mus.
3. fertilising
Farming China 9 belongs to large spike crossbreed, anti-rich water; Fertilizer should be used sufficient or bio-fertilizer is made base fertilizer; Pure N consumption is 18.0 kilograms/mu between breeding time, P 2O 510.0 kilograms/mu of consumptions, K 210.0 kilograms/mu of O consumptions.Application process: 1/3 nitrogenous fertilizer and whole phosphorus, base fertilizer made by potash fertilizer or seed manure applies, and 2/3 nitrogenous fertilizer topdresses, and applies in the typhon mouth phase.
4. insect protected
The corn lobus cardiacus will carry out the control of maize borer latter stage.

Claims (9)

1, a kind of method of hybrid maize seed production comprises the seed selection inbred line, and safety is isolated, and regulates date of seeding, suitable row ratio, roguing, the maternal castration rooted out male parent, gather in the crops, it is characterized in that: utilize inbred line CH584 to be female parent, inbred line CH361 is a paternal hybrid in good time, the preparation corn hybrid seed.
2, the method for claim 1 is characterized in that, described inbred line CH584 combines triple hybrid with (7922 * 8112) and LD143 hybridization, adopts pedigree method, through continuous 8 generation selfed breeding form.
3, the method for claim 1 is characterized in that, described inbred line CH361 is hybridized the crossbreed of assembly, F with red 599 and red 340 1In generation, selected open waterlogging-resistant, the disease-resistant fine individual plant of nature, adopts pedigree method, through continuous 8 generation selfed breeding form.
4, the method for claim 1, it is characterized in that described safety isolation, adopt distance isolation or time to isolate, adopt distance isolation to require not plant other corn more than 300 meters around the production of hybrid seeds district, adopt in the time insulation request production of hybrid seeds district sowing time will with other corn mistake phase more than 35 days.
5, the method for claim 1 is characterized in that described adjusting date of seeding, and CH584 does maternal, and CH361 makes male parent, broadcast the first phase during male parent earlier male parent seed soaking in three days in advance, sow simultaneously with female parent, broadcast second phase male parent again after first phase male parent is emerged; Or broadcast first phase male parent earlier, and when the first phase, male parent went out root, broadcast female parent, after first phase male parent is emerged, broadcast second phase male parent again.
6, the method for claim 1 is characterized in that described suitable row than being father, maternal going than adopting 1: 5.
7, the method for claim 1 is characterized in that described roguing adopts in seedling stage, shooting stage, takes out male early stage and all do not pull out meeting the assorted strain of parent's characteristic feature characteristic, different strain, lopsided strain, diseased plant and weak strain.
8, the method for claim 1 is characterized in that described maternal the castration, adopts and takes off luxuriant emasculating method in advance, thoroughly extracts maternal tassel.
9, the method for claim 1 is characterized in that the described male parent of rooting out is after the female parent pollination in production of hybrid seeds district finishes, and male parent is all rooted out.
CN 200710117811 2007-06-25 2007-06-25 Corn hybrid breeding method Pending CN101081003A (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104885910A (en) * 2014-09-29 2015-09-09 安徽盛创农业科技有限公司 High-yield hybrid maize seed production method
CN105028174A (en) * 2015-05-20 2015-11-11 阮积恩 High-yield corn hybridization breeding method
CN106069737A (en) * 2016-07-14 2016-11-09 合肥扬扬农业科技有限公司 A kind of high-yield hybrid producing method for seed of Semen Maydis
CN108184654A (en) * 2018-02-09 2018-06-22 山东省农业科学院玉米研究所(山东省农业科学院玉米工程技术研究中心) A kind of preparation method of Maize hybridized combination
CN108935080A (en) * 2018-08-20 2018-12-07 云南北玉种子科技有限公司 Corn breeding method
CN111758556A (en) * 2020-07-20 2020-10-13 通化市农业科学研究院 Breeding method of maize inbred line 29396 with high volume weight and high combining ability

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104885910A (en) * 2014-09-29 2015-09-09 安徽盛创农业科技有限公司 High-yield hybrid maize seed production method
CN105028174A (en) * 2015-05-20 2015-11-11 阮积恩 High-yield corn hybridization breeding method
CN106069737A (en) * 2016-07-14 2016-11-09 合肥扬扬农业科技有限公司 A kind of high-yield hybrid producing method for seed of Semen Maydis
CN108184654A (en) * 2018-02-09 2018-06-22 山东省农业科学院玉米研究所(山东省农业科学院玉米工程技术研究中心) A kind of preparation method of Maize hybridized combination
CN108935080A (en) * 2018-08-20 2018-12-07 云南北玉种子科技有限公司 Corn breeding method
CN111758556A (en) * 2020-07-20 2020-10-13 通化市农业科学研究院 Breeding method of maize inbred line 29396 with high volume weight and high combining ability

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Open date: 20071205