CN105918105B - A method of widening leaf mustard A subgenome hereditary variations using Chinese cabbage - Google Patents
A method of widening leaf mustard A subgenome hereditary variations using Chinese cabbage Download PDFInfo
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- CN105918105B CN105918105B CN201610269977.2A CN201610269977A CN105918105B CN 105918105 B CN105918105 B CN 105918105B CN 201610269977 A CN201610269977 A CN 201610269977A CN 105918105 B CN105918105 B CN 105918105B
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- leaf mustard
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
Abstract
A method of leaf mustard A subgenome hereditary variations being widened using Chinese cabbage, the method includes utilizing black mustard BnBnWith leaf mustard AjAjBjBjSexual hybridization obtains triploid seed A through Ovary culturejBjBn, triploid seed AjBjBnAllohexaploid A is obtained by chromosome doubling after aseptic condition is sproutedjAjBjBjBnBn, by the allohexaploid and Chinese cabbage Ar1‑nAr1‑nHybridization, obtains novel leaf mustard AjAr1‑nBjBn.Whole operation Period Process of the present invention is short, the method that can quickly and easily widen existing leaf mustard A subgenome hereditary variations.
Description
Technical field
The invention belongs to plant new lines breeding technique fields, and in particular to be a kind of with using black mustard and existing leaf mustard
Allohexaploid be bridge, a kind of novel leaf mustard of acquisition, specially a kind of profit hybridizes with a variety of Chinese cabbages of rich hereditary variation
The method for widening leaf mustard A subgenome hereditary variations with Chinese cabbage.
Background technology
Mustard crop (Brassica juncea, AABB) includes important vegetables and oil class crop type, including
The types such as hot pickled mustard tuber, youngster's dish, cabbage head, root-mustard and mustard type rape, wherein mustard type rape are commonly called as oily hot food, and seed is tiny,
Mostly golden yellow or kermesinus, oil content are high.Mustard type rape has the characteristics that barren tolerance is strong, but low output, at present growing surface
Product is smaller and smaller.Mustard type rape (Brassica juncea, AABB) is by turnip type rape (B.rapa, AA) and black mustard
(B.nigra, BB) natural hybridization, the allotetraploid that chromosome doubling is evolved.Compared with turnip type rape, juncea oil
The narrow hereditary basis of dish limits its genetic improvement.
Tradition is improved using Chinese cabbage there are mainly two types of the methods of leaf mustard：The first is hybridized by Chinese cabbage and black mustard, is created
Artificial synthesized leaf mustard；Second method is that leaf mustard hybridizes with Chinese cabbage, and Triploid is harvested under natural conditions, then utilizes three
Times body hybrid takes turns backcrossing more with leaf mustard, from backcross progeny selection and breeding contain the leaf mustard of Chinese cabbage genetic background.
First method due to Chinese cabbage and black mustard crossing barrier, in the approach each cross combination have to pass through largely
Embryo rescue techniques are likely to obtain cenospecies, and artificial synthesized leaf mustard Genomic instability can not be used directly, conservation there is also
Difficult (.Euphytica.2007 such as Wen).
In force, Triploid is only capable of using for the present age second method, can not conservation, and triploid and leaf mustard return
Friendship offspring is aneuploid, needs mostly to be likely to obtain the individual of juncea for cumbersome selection and breeding.
Invention content
The technical problem to be solved by the present invention is to widen the method for leaf mustard hereditary variation due to species using existing acquisition
Between serious reproduction affine sexual dysfunction is isolated, sexual hybridization is cannot achieve, even if being only suitable only for using if a large amount of rescue culture
A small amount of parent plants the crossing performance of material；And the species selection and breeding period is long, Breeding Process is cumbersome.It is a kind of present invention aims at providing
The method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage, using black mustard and leaf mustard sexual hybridization, Ovary culture rescue culture
Allohexaploid is obtained by chromosome doubling using the hexaploid as bridge to hybridize with Chinese cabbage, obtains novel leaf mustard, solves
The technological deficiencies such as prior art leaf mustard hereditary variation method is of limited application, and the selection and breeding period is long, Breeding Process is cumbersome.
The present invention's is achieved through the following technical solutions：
A method of leaf mustard A subgenome hereditary variations being widened using Chinese cabbage, the method includes utilizing black mustard BnBnWith
Leaf mustard AjAjBjBjSexual hybridization obtains triploid seed A through Ovary culturejBjBn, triploid seed AjBjBnAseptic condition is sprouted
Allohexaploid A is obtained by chromosome doubling afterwardsjAjBjBjBnBn, by the allohexaploid and Chinese cabbage Ar1-nAr1-nHybridization, is obtained
Obtain novel leaf mustard AjAr1-nBjBn。
Inventor has found through test of many times, in a kind of existing improvement method of leaf mustard using Chinese cabbage, Chinese cabbage Ar1-nAr1-nWith black mustard
BnBnHybridization, due to Chinese cabbage Ar1-nAr1-nWith black mustard BnBnThere are serious reproductions, and affine sexual dysfunction is isolated between two species, uses
Embryo rescue techniques overcome the affine sexual dysfunction between species, but artificial synthesized leaf mustard is unstable, can not directly use and conservation,
And need to carry out rescue culture steps of taking turns more in operating process and could obtain cenospecies, this method complexity is cumbersome, cannot be rapidly and efficiently
Filter out leaf mustard A subgenomes individual.
Another existing method is to utilize leaf mustard AjAjBjBjWith Chinese cabbage Ar1-nAr1-nHybridize, three times are harvested under natural conditions
Body hybrid AjAr1-nBj, Triploid AjAr1-nBjWith leaf mustard AjAjBjBjMore wheel backcrossings, from backcross progeny selection and breeding contain Chinese cabbage
The leaf mustard of genetic background, but the Triploid A obtained in this methodjAr1-nBjBe only capable of contemporary use, and can not conservation, three
Times body hybrid and leaf mustard AjAjBjBjBackcross progeny aneuploid needs that mostly leaf mustard A subgenomes could be obtained for cumbersome selection and breeding
Individual.
Based on defect caused by both the above existing method, inventor recognizes that selection economical character is excellent through research
Leaf mustard AjAjBjBjWith black mustard BnBnSexual hybridization obtains hybrid embryo AjBjBn, Ovary culture is carried out to hybrid embryo after pollinating 7-10 days
Rescue culture can overcome black mustard BnBnWith leaf mustard AjAjBjBjAffine sexual dysfunction, specific rescue culture method is isolated in reproduction between two species
It is to take Ovary culture to obtain hybrid embryo A after sexual hybridizationjBjBn, the triploid A of acquisitionjBjBnSeedling passes through chromosome doubling
Obtain allohexaploid AjAjBjBjBnBn, allohexaploid AjAjBjBjBnBnIt is fertile under field conditions (factors), can Reusability, can protect
Kind；The allohexaploid A of acquisitionjAjBjBjBnBnWith Chinese cabbage Ar1-nAr1-nCross compatibility is preferable, can get under the natural conditions of field
Filial generation.
In the method that inventor is studied, compared with the first existing operating method, select the species of hybridization different, species
The sequence of successively hybridization is different, and can be obtained leaf mustard A merely through a wheel ovary culturing embryo redemption and chromosome doublingjAjBjBj
With black mustard BnBnHexaploid hybrid AjAjBjBjBnBn, solve and need to carry out a large amount of embryo in the first existing operating method to draw
It rescues technology and obtains cenospecies；The novel leaf mustard inheritance stability obtained using the method for the invention, can directly be cultivated from the present age
Excellent leaf mustard A sub-gene individuals of shape in selection and breeding in leaf mustard, and cultivate it is novel can be used directly, can conservation, solve
Artificial synthesized leaf mustard Genomic instability, can not directly use, the problem of conservation difficulty in the first existing operating method.
Compared with second of operating method, select the species of hybridization different, the sequence of species successively hybridization is different, entire to grasp
Make method difference, can be found by experiment that the method for the invention in contrast, solving it needs mostly for cumbersome selection and breeding leaf mustard individual
The problem of, and the present invention is easy to operate, while the leaf mustard A subgenomes individual obtained using the method for the invention can be made in mostly generation
With, do not limit only the present age use, can conservation, selection and breeding program is simple, and the whole process period is short, can quickly and easily widen existing mustard
Dish A sub-genes hinder hereditary variation.
The present invention specific operation process be：
(1) hexaploid is obtained
Choose the excellent leaf mustard A of agronomic shapejAjBjBjFor female parent, flower bud emasculation is shelled, is awarded with black mustard BnBnPollen, pollinate 7-10
Rescue culture Ovary culture is carried out to hybrid ovary after it, the seedling that rescue culture mature seed is sprouted is placed in differential medium and is expanded
Numerous 2 wheel, often wheel culture 25 days are differentiated a large amount of clone, are then doubled using colchicin induced chromosome, obtain gene
Group structure is AjAjBjBjBnBnAllohexaploid or with black mustard BnBnFor female parent, flower bud emasculation is shelled, is awarded with leaf mustard AjAjBjBjPollen is awarded
Powder carries out rescue culture Ovary culture after 7-10 days to hybrid ovary, and the seedling that rescue culture mature seed is sprouted is placed in differentiation culture
Expand numerous 2 wheel in base, often wheel culture 25 days is differentiated a large amount of clone, then doubled, obtained using colchicin induced chromosome
It is A to obtain genome structurejAjBjBjBnBnAllohexaploid；
(2) novel leaf mustard A subgenomes individual is created
With allohexaploid AjAjBjBjBnBnAs female parent, artificial emasculation authorizes Chinese cabbage Ar1-nAr1-nPollen, field are natural
Under the conditions of grow, until harvest seed is to get novel leaf mustard A subgenomes individual or with Chinese cabbage A after riper1-nAr1-nFor female parent, award
Give allohexaploid AjAjBjBjBnBnPollen, grow under the natural conditions of field, until harvest seed is to get novel leaf mustard after ripe
A subgenome individuals.
It is A that the wherein described leaf mustard, which is genome,jAjBjBjCrop, including mustard type rape and vegetables are with leaf mustard, described white
Dish is that genome is Ar1-nAr1-nCrop, including turnip type rape and vegetables use Chinese cabbage.
Wherein, j, r, n represent the source of chromosome, AjA chromosome group i.e. in leaf mustard B.juncea, ArIt is exactly to come
From the A chromosome group of Chinese cabbage B.rapa, BnIt is exactly the B chromosome group from black mustard B.nigra, BjIt is exactly to come from leaf mustard B.nigra
B chromosome group.
Wherein obtain the triploid A of sproutingjBjBnThe specific method of seedling is：Win the triploid after hybridization 7-10 days
AjBjBnOvary sterilizes 30s, sterile water wash 5min through 70% alcohol, then uses 10% hypochlorite disinfectant 15min, it is average often
3min rocks once, then uses sterile water wash 3 times, each 3min, removes rataria, is inserted into ovary carpopodium position connects later
Kind in MS culture mediums, being positioned over illumination in 16 hours, 8 hours dark treatments are cultivated in 20 DEG C of illumination cultivation room, 30-35 days receipts
Obtain ripe silique, peel angle pericarp under aseptic condition obtains triploid AjBjBnSeed.
To the triploid A of sproutingjBjBnSeedling carries out chromosome doubling concrete operation step：Triploid A will be obtainedjBjBn
Seed, which is placed in MS culture mediums, to be sprouted, and seedling handles the asexual seedling obtained through differential medium and is transferred to containing 100mg/L after sprouting
The differential medium light culture induced chromosome of colchicin doubles processing 7 days, and asexual seedling is transferred to differential medium later
25 days seedlings of middle culture, are finally transferred in root media and take root.Transplanted after taking root, detection plant pollen fertility, robustness or
It is doubled by chromosome counting screening successfully individual.
In above-mentioned sprouting triploid AjBjBnSeedling, triploid seedling carry out employed in the operating procedure of chromosome doubling
Root media it is composed of the following components：MS culture medium+0.5mg/L IBA；Differential medium is composed of the following components, MS trainings
Support base+2mg/L IBA+0.2mg/L NAA.
Leaf mustard described in the content of present invention is the crop that genome is AABB, including mustard type rape and vegetables use mustard
Dish, the Chinese cabbage are the crop that genome is AA, including turnip type rape and vegetables use Chinese cabbage.
Compared with prior art, the present invention having the following advantages and advantages：
The present invention passes through leaf mustard AjAjBjBjWith black mustard BnBnHybridization passes through a wheel rescue culture Ovary culture and chromosome doubling
Obtain allohexaploid AjAjBjBjBnBn, and with hexaploid AjAjBjBjBnBnAs bridge, hexaploid AjAjBjBjBnBnNatural item
It is fertile under part, can Reusability, a variety of Chinese cabbage A with rich hereditary variationr1-nAr1-nCompatibility is preferable, under the natural conditions of field
It can get filial generation.Whole operation Period Process is short, can quickly and easily widen existing leaf mustard A subgenome hereditary variations
Method.
Specific implementation mode
To make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiment, the present invention is made
Further to be described in detail, exemplary embodiment of the invention and its explanation are only used for explaining the present invention, are not intended as to this
The restriction of invention.
Embodiment 1：
A method of leaf mustard A subgenome hereditary variations being widened using Chinese cabbage, are included the following steps：
With strain be PI174795 black mustard it is maternal, shells flower bud emasculation, it is 4N001 leaf mustard pollen to authorize strain, obtains three times
Body F1, pollination carry out first round rescue culture Ovary culture to triploid F1 hybrid ovarys after the 10th day, obtain triploid seed, sprout
Seedling after hair is doubled by colchicin induced chromosome, obtains allohexaploid D1；It is female parent, people with allohexaploid D1
3 parts of core Chinese cabbages, the flower of strain respectively 1. 4M242,2. 5W244,3. 5W246 and 2 part of turnip type rape are authorized in work emasculation
Powder, respectively 4. 5W395,5. 6Y733, hybrid ovary are grown strain under field conditions (factors), are harvested after ripe.
Allohexaploid D1 in embodiment is hybridized with 5 kinds of different types of Chinese cabbages, the chromosome of the filial generation of acquisition
Data are detected, and chromosome number is 36.Then setting percentage hexaploid hybridized with the Chinese cabbage of 5 kinds of numbering types into
Row statistics, statistical result are as shown in table 1.The novel leaf mustard obtained after above-mentioned different numbering type Chinese cabbage hybridization is trained simultaneously
It educates, a variety of agronomic shapes such as blade profile, pattern, plant height, number of branches and the form of above-mentioned different types of novel leaf mustard, florescence
Difference is apparent, and hereditary variation is larger.The present embodiment is also selfed using above-mentioned different types of novel leaf mustard, to new after selfing
The setting percentage of type leaf mustard is counted, and statistical result is as shown in table 1.
Table 1
| Leaf mustard is numbered | Cross fertile rate (grain/pod) | Self-fruitful rate (grain/pod) |
| ① | 3.32 | 4.63 |
| ② | 2.54 | 5.54 |
| ③ | 2.99 | 5.73 |
| ④ | 0.98 | 3.22 |
| ⑤ | 1.84 | 4.80 |
Above-mentioned hexaploid D1 hybridizes with different type Chinese cabbage, and the setting percentage that is averaged is 2.33/pod, and above-mentioned novel leaf mustard is certainly
After friendship, the setting percentage that is averaged is 4.78/pod.
Embodiment 2：
The present embodiment and embodiment 1 difference lies in：With strain be 4N001 leaf mustard in the present embodiment it is maternal, with strain
It is male parent for PI174795 black mustard, hybridization, pollination carries out Ovary culture on the 10th day to hybrid ovary, obtains triploid seed, sprouts
Triploid F2 is obtained after hair, through first round rescue culture Ovary culture, the seedling of acquisition is induced by colchicin to be contaminated triploid F2
Doubling of chromosome obtains allohexaploid D2；It is female parent with allohexaploid D2, artificial emasculation authorizes 3 parts of core Chinese cabbages, product
System is respectively the pollen of 1. 4M242,2. 5W244,3. 5W246 and 2 part of turnip type rape, and strain is respectively 4. 5W395,5.
6Y733, hybrid ovary are grown under field conditions (factors), and different types of novel leaf mustard is harvested after ripe.
The chromosome number of novel leaf mustard after hybridizing to the present embodiment is detected, it is found that their chromosome number is equal
It is 36, a variety of agronomic shapes such as blade profile, pattern, plant height, number of branches and the form of above-mentioned more parts of novel leaf mustard, florescence
Difference is apparent, and hereditary variation is larger.
It is average solid after hybridizing with different type Chinese cabbage according to identical detection method detection hexaploid D2 in embodiment 1
Average setting percentage after rate and different types of novel leaf mustard selfing, which is 2.78/pod, should be from knot
Real rate is 4.82/pod.
Embodiment 3：
The present embodiment and embodiment 1 difference lies in, for the present embodiment using allohexaploid D1 as male parent, Chinese cabbage is female parent,
Chinese cabbage type is in the same manner as in Example 1, and novel leaf mustard is obtained after hybridization.
The chromosome number of novel leaf mustard after hybridizing to the present embodiment is detected, it is found that their chromosome number is equal
It is 36, a variety of agronomic shapes such as blade profile, pattern, plant height, number of branches and the form of above-mentioned more parts of novel leaf mustard, florescence
Difference is apparent, and hereditary variation is larger.
It is average solid after hybridizing with different type Chinese cabbage according to identical detection method detection hexaploid D1 in embodiment 1
Average setting percentage after rate and different types of novel leaf mustard selfing, which is 2.48/pod, should be from knot
Real rate is 4.90/pod.
Embodiment 4：
The present embodiment the difference from example 2 is that：Using allohexaploid D2 as male parent in the present embodiment, Chinese cabbage is mother
This, Chinese cabbage type is same as Example 2, the novel leaf mustard obtained after hybridization.
The chromosome number of the novel leaf mustard obtained after hybridizing to the present embodiment is detected, and finds their chromosome number
Mesh is 36, a variety of agronomy such as blade profile, pattern, plant height, number of branches and the form of above-mentioned more parts of novel leaf mustard, florescence
Shape difference is apparent, and hereditary variation is larger.
It is average solid after hybridizing with different type Chinese cabbage according to identical detection method detection hexaploid D2 in embodiment 1
Average setting percentage after rate and different types of novel leaf mustard selfing, which is 2.50/pod, should be from knot
Real rate is 4.65/pod.
Embodiment 5：
The present embodiment and embodiment 1 difference lies in, the strain of black mustard and leaf mustard is different in the present embodiment, other with reality
It is identical to apply example 1.
The strain of black mustard is PI174796 in the present embodiment, and the strain of leaf mustard is 4N001.According to identical in embodiment 1
Detection method detects the average setting percentage after hexaploid D3 hybridizes with different type Chinese cabbage and different types of novel leaf mustard certainly
Average setting percentage after friendship, the Cross fertile rate are 2.75/pod, which is 4.82/pod.
The chromosome number of the novel leaf mustard obtained after hybridizing to the present embodiment is detected, testing result chromosome number evidence
It is 36, a variety of agricultures such as the blade profile of more parts of novel leaf mustard, pattern, plant height, number of branches and form, florescence in the present embodiment
Skill shape difference is apparent, and hereditary variation is larger.
Above-described specific implementation mode has carried out further the purpose of the present invention, technical solution and advantageous effect
It is described in detail, it should be understood that the foregoing is merely the specific implementation mode of the present invention, is not intended to limit the present invention
Protection domain, all within the spirits and principles of the present invention, any modification, equivalent substitution, improvement and etc. done should all include
Within protection scope of the present invention.
Claims (8)
1. a kind of method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage, it is characterised in that：The method includes utilizing
Black mustard BnBnWith leaf mustard AjAjBjBjSexual hybridization obtains triploid seed A through Ovary culturejBjBn, triploid seed AjBjBnThrough
Allohexaploid A is obtained by chromosome doubling after aseptic condition sproutingjAjBjBjBnBn, by the allohexaploid and Chinese cabbage
Ar1-nAr1-nHybridization, obtains novel leaf mustard AjAr1-nBjBn, the triploid that will be sprouted using rescue culture Ovary culture method
AjBjBnSeedling, which is placed in differential medium, expands numerous 2 wheel, and often wheel culture 25 days differentiates asexual seedling, then leads to asexual seedling
It crosses colchicin induced chromosome to double, the triploid A of the sproutingjBjBnThe preparation method of seedling is：Win hybridization 7-10
Triploid A after itjBjBnOvary sterilizes 30s, sterile water wash 5min through 70% alcohol, then with 10% hypochlorite disinfectant
15min, it is average to be rocked once per 3min, then use sterile water wash three times, each 3min, then by the carpopodium position of ovary
It is inserted into stripping rataria, is inoculated into MS culture mediums, is positioned over illumination in 16 hours, 8 hours dark treatments, in 20 DEG C of illumination cultivation room
It cultivates, the ripe silique of 30-35 days harvests, peel angle pericarp under aseptic condition obtains triploid AjBjBnSeedling seed.
2. a kind of method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage according to claim 1, feature exist
In：The chromosome doubling concrete operation step is：By the triploid A of acquisitionjBjBnSeed, which is placed in MS culture mediums, to be sprouted, and is sprouted
Seedling, which is transferred in differential medium, after hair expands numerous 2 times, and the clone seedling of acquisition, is transferred to after processing and contains by culture 25 days every time
Light culture induced chromosome doubles processing 7 days in the differential medium of 100mg/L colchicins, then will asexual that treated is young
Seedling, which is transferred in differential medium, cultivates 25 days seedlings, is finally transferred in root media and takes root, is transplanted after taking root, and detects plant
Powder fertility, robustness double successfully individual by chromosome counting screening.
3. a kind of method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage according to claim 2, feature exist
In：The root media is composed of the following components：MS culture medium+0.5mg/L IBA；Differential medium is by following components group
At：MS culture medium+2mg/L IBA+0.2mg/L NAA.
4. a kind of method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage according to claim 1, feature exist
In：The black mustard BnBnFor female parent, flower bud emasculation is shelled, is awarded with leaf mustard AjAjBjBjPollen, sexual hybridization obtain three times through Ovary culture
Body seed AjBjBn, triploid seed AjBjBnChromosome doubling obtains allohexaploid AjAjBjBjBnBn, by described heterologous six times
Body AjAjBjBjBnBnWith Chinese cabbage Ar1-nAr1-nHybridization, obtains novel leaf mustard AjAr1-nBjBn。
5. a kind of method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage according to claim 1, feature exist
In：The leaf mustard AjAjBjBjFor female parent, flower bud emasculation is shelled, is awarded with black mustard BnBnPollen, sexual hybridization obtain three times through Ovary culture
Body seed AjBjBn, triploid seed AjBjBnDyed body doubles to obtain allohexaploid AjAjBjBjBnBn, by described heterologous six
Times body AjAjBjBjBnBnWith Chinese cabbage Ar1-nAr1-nHybridization, obtains novel leaf mustard AjAr1-nBjBn。
6. a kind of method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage according to claim 1, feature exist
In：The allohexaploid A obtained with chromosome doublingjAjBjBjBnBnFor female parent, flower bud emasculation is shelled, is awarded with Chinese cabbage Ar1-nAr1-n
Pollen obtains novel leaf mustard AjAr1-nBjBn。
7. a kind of method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage according to claim 1, feature exist
In：It is described with Chinese cabbage Ar1-nAr1-nFor female parent, flower bud emasculation is shelled, the allohexaploid A obtained with chromosome doubling is awardedjAjBjBjBnBn
Pollen obtains novel leaf mustard AjAr1-nBjBn。
8. a kind of method for widening leaf mustard A subgenome hereditary variations using Chinese cabbage according to claim 1, feature exist
In：The leaf mustard is the crop that genome is AABB, including mustard type rape and vegetables, with leaf mustard, the Chinese cabbage is that genome is
The crop of AA, including turnip type rape and vegetables use Chinese cabbage.
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| "A large-scale introgression of genomic components of Brassica rapa into B. napus by the bridge of hexaploid derived from hybridization between B. napus and B. oleracea";Qinfei Li等;《Theor Appl Genet》;20130523;图1以及第2074页左栏第2-3段以及引用文件"Improving ovary and embryo culture techniques for effcient resynthesis of Brassica napus from reciprocal crosses between yellow-seeded diploids B. rapa and B. oleracea"，Jing wen等，Euphytica，第162期，第81-89页，2008年12月31日中材料与方法以及结果部分 * |
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