CN108243949A - A kind of selection of portable temperature-sensitive sterile line - Google Patents
A kind of selection of portable temperature-sensitive sterile line Download PDFInfo
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- CN108243949A CN108243949A CN201810217681.5A CN201810217681A CN108243949A CN 108243949 A CN108243949 A CN 108243949A CN 201810217681 A CN201810217681 A CN 201810217681A CN 108243949 A CN108243949 A CN 108243949A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention discloses a kind of methods of selection and breeding portable long-grained nonglutinous rice temperature-sensitive sterile line.This method includes the following steps：The measure of fertility screening-Fertility observation-combo advantage that selection-hybridization of parent material, selfing-multi-method are combined, the sword-like leave for obtaining polymerizeing big openness angle of flag leaf gene stretch rice indica hybrid temperature-sensitive sterile line obliquely downward.When the sword-like leave formulated using this method stretches the temperature-sensitive sterile line progress rice production of hybrid seeds obliquely downward, do not reduce match combination Yield Competition advantage under the premise of, the link of leaf is manually cut when saving current breeding of hybrid rice, reduce the amount of labour, reduce labor intensity, it avoids cutting the influence that leaf brings rice plant normal growth simultaneously, realizes that the green of hybrid rice is light and simplify the production of hybrid seeds.
Description
Technical field
The invention belongs to rice heterosis utilization new material innovation areas, and in particular to a kind of temperature sensitive Genetic Sterility of portable
The selection of system.
Background technology
Hybrid rice plants the history of nearly 40 years in China, at present 16,000,000 hm of annual planting area2Left and right, more than rice
The 50% of cultivated area has played increases in grain production huge effect, and gradually develops to more than 20 country.A paddy rice hybrid generation
The production of seed needs artificial supplementary pollination to improve outcrossing seed-setting rate.To remove obstacle of pollinating, usually male sterile line
Sword-like leave prescind top 1/3-1/2.Cutting leaf not only needs the labour of high intensity, but also needs higher operating technology in order to avoid injuring
The tender spike of rice of children.The normal growth of rice plant is also adversely affected in addition, cutting wound caused by leaf.This seminar is in Anhui
A sword-like leave tiltedly downwardly extending Local Indica Rice Anhui extensive 101 is found that in square variety source.Extensive 101 time of infertility 130d in Anhui, strain
High 131cm, 150 ° or so of the angle between sword-like leave blade and spike of rice, sword-like leave is in stretch obliquely downward, and the rest blades such as two leaves are same
Normal rice is equally lifted in tiltedly upper.Through multiple years（Hefei, Lingshui）Field observation, the trait expression stablize.
Angle of rice flag leaf refers to the size of angle between full heading time sword-like leave blade and spike of rice.By Flag Leaves in Rice in this method
Angle is defined as sword-like leave when being more than 90 ° and stretches obliquely downward, and openness angle of flag leaf is bigger, and tiltedly downwardly extending degree is bigger for sword-like leave.So far, it is right
The genetic research of Flag Leaves in Rice morphological characters is focused primarily upon in terms of improving the plant type raising efficiency of light energy utilization, and majority is to sword-like leave
The research of length and width, length-width ratio and sword-like leave area character, and the research report about angle of rice flag leaf is less.From Shen Fucheng
（1983）, Yan Yueming etc.（1990）Dong Guojun etc. is arrived to the genetic analysis of openness angle of flag leaf（2003）To controlling the QTL of openness angle of flag leaf
Positioning is carried out until Wang get Kai etc.（2005）Deng to big openness angle of flag leaf genellaClone, its sword-like leave angle of researcher's material therefor
Degree is respectively less than 90 °, is lifted in tiltedly upper, practical significance is had no to breeding of hybrid rice.Therefore, study, excavate and utilize new rice
Germ plasm resource, especially big openness angle of flag leaf（Openness angle of flag leaf is more than 90o）Sword-like leave stretch resource obliquely downward, rice genetic basis is ground
Study carefully, Breeding Application research and its separation of related gene clone are of great significance.
Anhui extensive 101 seedling stage, tillering stage, jointing stage all blades are tiltedly upper lift, but sword-like leave pulvinus as other kinds
It is higher by down after two leaf pulvinus（That is boot stage）Sword-like leave starts to stretch obliquely downward, 150 ° of openness angle of flag leaf, and rest blade still it is normal tiltedly on
It lifts.It, can be with the free combo of rice varieties, selection and breeding double-linear hybrid rice by big openness angle of flag leaf channel genes temperature-sensitive sterile line
New varieties.It stretches temperature-sensitive sterile line obliquely downward using sword-like leave and carries out breeding of hybrid rice, the link that male sterile line cuts leaf can be exempted,
150 yuan of human cost is reduced by seed farm per acre（1 workday）Meter, annual about 2,500,000 mu of the production of hybrid seeds area in the whole nation, can reduce system
About 3.75 hundred million yuan of cost of kind.Moreover, with the development of national economy and society, rural laborer largely shifts to city,
It is important that the laborsaving portable seeding technique of hybrid rice ensures that national food security also has for sustainable utilization rice heterosis
Meaning.
Invention content
The object of the present invention is to provide a kind of method of portable long-grained nonglutinous rice temperature-sensitive sterile line selection and breeding, which has sword
Leaf is stretched obliquely downward（Openness angle of flag leaf is more than 120o）Hereditary capacity, other characters are similar to same type temperature-sensitive sterile line, can with it is normal
Advise the free combo of rice varieties, selection and breeding double-linear hybrid rice new varieties.
The present invention provides a kind of methods of portable long-grained nonglutinous rice temperature-sensitive sterile line selection and breeding.This method includes the following steps：
（1）Using from Germplasm Resources of Local Indica Rice In Anhui Anhui extensive 101（Openness angle of flag leaf 150o）With from peace agriculture S（As extensively
Jasmine S, Guangzhan 63S etc.）And 1892S（Short purple S, Z913S etc.）Rice indica hybrid temperature-sensitive sterile line hybridization, selfing, obtain not
With the F of combination2For seed；
（2）Each combination F2It plants 3000 plants or so respectively for group, therefrom screens openness angle of flag leaf and be more than 120o plant heights, short in 85cm
Temperature sensitive Genetic Sterility single plant；
（3）Screening garden is established, in low temperature（23.0-23.5℃）Under environmental pressure, pass through pollen microscopic examination and Fertility observation, choosing infertility
The low sword-like leave of origin temp stretches single plant obliquely downward（Openness angle of flag leaf is more than 120o）, it is selfed until character stabilization, makes the low starting point temperature of control
The sterile gene of degree polymerize with big openness angle of flag leaf genetic recombination；
（4）Through identification of male sterility, it is bred as indica type temperature sensitive core that big openness angle of flag leaf gene is integrated in one with temperature sensitive genic male sterile gene not
It educates and is.
In the selection of the present invention,（1）Used temperature-sensitive sterile line is that current production is upper widely applied indica type
The main source of temperature-sensitive sterile line fertile gene is the genetic tool of double-linear hybrid rice.
In the selection of the present invention, for the ease of（2）In mutative material acquisition, the same period will plant a certain number of
Parents plant is as control, and by F2Group's openness angle of flag leaf is divided into different brackets.
In the selection of the present invention, step（1）In two different kind of material hybridization, step（2）In sword-like leave angle
Spend differential screening and step（3）In artificial environment pressure stress, it is therefore an objective to make the sterile gene of the low origin temp of control with it is big
Openness angle of flag leaf genetic recombination polymerize in one, so that the sword-like leave for obtaining practical stretches temperature-sensitive sterile line obliquely downward.
In the selection of the present invention, routine techniques may be used in unspecified method.Such as sterile line fertility point
The observation of phase sowing, the identification of pollen abortion types, the investigation of bagging self-fruitful rate, the emasculation of lukewarm water method, growth cabinet are low
Stubble breeding etc. is cut in temperature processing.
The rice that the selection of the present invention obtains is the temperature sensing male core that a kind of sword-like leave is stretched obliquely downward, critical temperature of sterility is low
Sterile line.
Thcrmo-scnsitivc genie male stcrility system refers to that a kind of fertility is controlled by recessive nuclear gene, and fertility is sent out under certain temperature conditions
Raw conversion, shows as pollen abortion degeneration, stigma development normal rice strain when sterile.
Fertility al-teration refers to show pollen debility, male sterility at relatively high temperatures, it is impossible to which self-fertility can only rely on
Foreign pollen fertilization produces hybrid seed in production using restorer and its combo by way of artificial pollination；And
Such material shows male-fertile under the conditions of temperature is relatively low, and self-fertility utilizes this characteristic progress breeding in production.
Fertility al-teration origin temp is the temperature value that the fertility of temperature-sensitive sterile line is instigated to change, and when higher temperature makes
The fertility of sterile line is known as critical temperature of sterility by the fertile temperature for turning to infertility.In production, the low infertility of critical temperature of sterility
Risk is smaller during system's application.
Restorer refers to a kind of normotrophic rice strain, miscellaneous with temperature-sensitive sterile line containing dominant restoring gene
It hands over and generates F1The fertility normal energy self-fertility in generation.
It is oblique available for cultivating new sword-like leave that the sword-like leave that the selection of the present invention obtains stretches Indica TGMS line obliquely downward
Under stretch temperature-sensitive sterile line；The sword-like leave that the selection of the present invention obtains stretches Indica TGMS line available for selection and breeding two obliquely downward
Linear hybrid rice new varieties.The either sword-like leave that the selection of the present invention obtains is stretched temperature-sensitive sterile line or is utilized obliquely downward
The two-line hybrid rice of the sterile line breeding, all with normal phenotype.Experiment proves that indica type sword-like leave stretches temperature-sensitive sterile line obliquely downward
Fertility al-teration is apparent, and critical temperature of sterility is low, and the sterile period is longer, and abortion is thorough, sterile degree 99.96%, and bagging selfing is not tied
Real rate 100%；For such sterile line in addition to sword-like leave is stretched obliquely downward, other characters are similar with same type temperature-sensitive sterile line, can be with routine
Rice varieties carry out free combo with selection and breeding double-linear hybrid rice new varieties；And its Hybrid Rice Combination prepared is effective in single plant
In fringe, every total grain panicle number, setting percentage and mass of 1000 kernel Isoquant character significant difference is had no with common two-line hybrid rice.
The selection of the present invention has following purposes in terms of double-linear hybrid rice is cultivated：
1st, using the sword-like leave of selection and breeding of the present invention stretch obliquely downward Indica TGMS line can cultivate new Flag Leaves in Rice stretch obliquely downward it is temperature sensitive
Line with genic sterile；
2nd, training of the Indica TGMS line available for double-linear hybrid rice new varieties is stretched obliquely downward using the sword-like leave of selection and breeding of the present invention
It educates；
3rd, it stretches to exempt when temperature-sensitive sterile line carries out breeding of hybrid rice obliquely downward using the sword-like leave of selection and breeding of the present invention and prescinds male
The link of sterile line sword-like leave, reduces labor intensity, and reduces the amount of labour, reduces breeding cost, realizes that the light of hybrid rice simplifies system
Kind.
Beneficial effects of the present invention：
The present invention provides a kind of methods for polymerizeing big openness angle of flag leaf gene selection and breeding sword-like leave and stretching long-grained nonglutinous rice temperature-sensitive sterile line obliquely downward.Rate
First big openness angle of flag leaf gene is polymerize with temperature sensitive genic male sterile gene in one, it can combo selection and breeding two at random so as to create one
The system of linear hybrid rice new varieties solves the link that male sterile line sword-like leave need to be prescinded when producing upper breeding of hybrid rice,
It reduces labor intensity, reduces the amount of labour, reduce breeding cost, realize that hybrid rice gently simplifies the production of hybrid seeds.
Specific embodiment
It stretches Indica Rice Restorer Lines obliquely downward using sword-like leave with temperature-sensitive sterile line material to be hybridized, gene is allowed freely to recombinate poly-
It closes, in segregating population, sword-like leave of the screening openness angle of flag leaf more than 120o stretches sterile material, then under ambient pressure obliquely downward, passes through
Big openness angle of flag leaf gene is aggregating by artificial selection with temperature sensitive genic male sterile gene, is cultivated sword-like leave and is stretched temperature-sensitive sterile line obliquely downward,
To achieve the purpose that hybrid rice gently simplifies the production of hybrid seeds.
Example 1：Sword-like leave stretches temperature-sensitive sterile line Breeding Process obliquely downward
1.1 materials and methods
To derive from Anhui Native kind Indica Rice Germplasm Anhui extensive 101 as male parent, to derive from peace agriculture S（Such as wide jasmine S, Guangzhan 63S
Deng）And 1892S（Short purple S, Z913S etc.）6 temperature-sensitive sterile lines make it is maternal；1,10 and 20 days December in 2011 will
Parent is seeded in Hainan Lingshui base three phases of one's duty, is transplanted after 28 days, 13.3 × 16.5cm of seeding row spacing；Treat that male parent Anhui extensive 101 is taken out
After tassel blossom, the single plant just eared but not yet bloomed is selected from temperature-sensitive sterile line, it is extensive with Anhui respectively using lukewarm water method emasculation
The hybrid seed of different temperature-sensitive sterile line combinations is prepared in 101 bagging artificial pollinations（Table 1）.
1 Anhui of table extensive 101 and different Indica TGMS line combo situations
| Combination | Maternal source | Paternal origin | Cenospecies quantity |
| Wide jasmine S/ Anhui extensive 101 | Rice institute of academy of agricultural sciences of Anhui Province | Rice institute of academy of agricultural sciences of Anhui Province | 54 |
| Guangzhan 63S/Anhui extensive 101 | Academy of agricultural sciences of Liaoning Province, Feng Le | Rice institute of academy of agricultural sciences of Anhui Province | 38 |
| New peace S/Anhui extensive 101 | Anhui an aromatic plant metioned in ancient books silver high-tech | Rice institute of academy of agricultural sciences of Anhui Province | 63 |
| New two S/Anhui extensive 101 | Rice institute of academy of agricultural sciences of Anhui Province | Rice institute of academy of agricultural sciences of Anhui Province | 45 |
| Short purple S/Anhui extensive 101 | Rice institute of academy of agricultural sciences of Anhui Province | Rice institute of academy of agricultural sciences of Anhui Province | 91 |
| Z913S/Anhui extensive 101 | Rice institute of academy of agricultural sciences of Anhui Province | Rice institute of academy of agricultural sciences of Anhui Province | 39 |
1.2 sword-like leaves stretch the selection and breeding of temperature-sensitive sterile line obliquely downward
（1）On April 27th, 2012 combines matched F by each1Seed is sowed at Hefei base, transplants May 27, and seeding row spacing 13.3 ×
16.5cm, mixed receive of same combination obtains F after heading is ripe2Seed；
（2）Winter in 2012 is by the F of each combination2Seed is planted in Hainan Lingshui base, the F each combined2Group's plantation 3000
Strain or so, therefrom selects that sword-like leave is stretched obliquely downward and sectional fructification may be the single plant of temperature sensitive Genetic Sterility is reserved seed for planting, 6 various combinations
F2Group is elected to 3-10 plants of single plant and differs；
（3）From after 2013, positive season is sowed in Hefei late April, and winter sowing at the beginning of Lingshui 12 months, such Hefei, Hainan are past
It returns and adds generation, it is each from generation to generation according to objective trait Superior line until strain is stablized；
（4）Elected stabilization strain carries out Fertility observation and identification using interval sowing and growth cabinet, until the autumn in 2017,
It is bred as Anhui jasmine 101S, the new 101S in Anhui, the indica type sword-like leave of Anhui purple 101S and Anhui 913S and stretches temperature-sensitive sterile line 4 obliquely downward.
Example 2：Sword-like leave stretches the fertility screening of temperature-sensitive sterile line obliquely downward under natural conditions
The strain that different sword-like leaves are stretched to temperature-sensitive sterile line obliquely downward is planted in selective nursery, and beginning fringe to full heading time selects the fringe that just begins
The single fringe not yet bloomed takes on tassel the grain husk flower anther of middle part branch stalk to carry out microscopy, during microscopy every grain husk spend in 6 anther wholes
It takes out, is placed on glass slide, the I-KI solution of drop appropriate 1% after anther is fully smashed to pieces dyeing with tweezers, is placed on 10 × 10 times
Micro- Microscopic observation pollen types；Similar single fringe set selfing bag is selected while sampling, self-fertility situation is checked after 20 days；According to
Microscopy and bagging self-fertility as a result, selected from hand over it is shaky, lose in the single plant of pollen grain and select without pollen or a small amount of allusion quotation.
Example 3：Fertility observation
Under the natural conditions of Hefei, began to cast to 12 end of day in July from April 12 within 2016, broadcasting for 1 phase per 10d, totally 10 phase, observation are each
Phase sword-like leave stretches the fertility dynamic of temperature-sensitive sterile line obliquely downward.Each issue kind is planted 100 plants or so, 13.3 × 16.5cm of seeding row spacing, the length of time rice seedlings grow
28 days；From beginning fringe（July 13）Start to heading to terminate, 5 fringe microscopy pollen were taken every 1-2 days, observe pollen abortion degree；Together
Shi Xuan investigates self-fruitful rate similar to 10 fringe baggings when ripe.The result shows that：July 13 days-September during 8 days microscopy, pollen lose
Rate 99.95%-100% is educated, shaky bagging selfing is 0, microscopy during September -23 days on the 12nd, pollen abortion rate 68.91%-
96.82%, bagging self-fruitful rate 13.75%-43.82% illustrate that the sword-like leave of observation stretches temperature-sensitive sterile line in Hefei infertility obliquely downward
Period is longer, and the infertility fertile transition period is apparent（Table 2）.
Sword-like leave stretches temperature-sensitive sterile line Fertility observation obliquely downward under 2 2016 years Hefei natural conditions of table
Example 4：Sword-like leave stretches temperature-sensitive sterile line combo advantage measure obliquely downward
（1）4 sword-like leaves stretch temperature-sensitive sterile line obliquely downward：Anhui jasmine 101S, the new 101S in Anhui, Anhui purple 101S and Anhui 913S；7 recoveries
System：Peace is No. 2 fragrant, peace perfume 15, R998, R727, middle Xian WH26, China account for, Yang No.6 rice；
（2）4 sword-like leaves are stretched temperature-sensitive sterile line and 7 restorer plantations in Lingshui base by December, 2016 obliquely downward, and heading is opened
Florescence stretches temperature-sensitive sterile line as female parent obliquely downward using 4 sword-like leaves, hot water emasculation, and 28 are obtained respectively at 7 restorer bagging pollinations
A cross combination；
（3）28 cross combinations and 2 controls are sowed at Hefei base, transplanting on June 12, seeding row spacing 16.5 by May 8th, 2017
× 26.5cm, this single plantation, maturity period carry out the investigation of yield traits, are as a result listed in table 3.
34 sword-like leaves of table stretch temperature-sensitive sterile line and match combination yield traits investigation obliquely downward
| Combination | Plant height/cm | The effective fringe of single plant | Per total grain panicle number | Setting percentage/% | Mass of 1000 kernel/g |
| Jasmine 101S/ peaces in Anhui are No. 2 fragrant | 125.2 | 11.5 | 236.4 | 82.5 | 26.6 |
| Anhui jasmine 101S/ peaces perfume 15 | 132.4 | 9.8 | 217.2 | 86.3 | 25.7 |
| Anhui jasmine 101S/R998 | 131.9 | 11.9 | 212.3 | 80.5 | 27.3 |
| Anhui jasmine 101S/R727 | 128.1 | 12.6 | 207.5 | 88.1 | 27.1 |
| Xian WH26 in the jasmine 101S/ of Anhui | 127.6 | 10.3 | 226.4 | 83.4 | 27.5 |
| Anhui jasmine 101S/ China accounts for | 126.5 | 10.8 | 229.9 | 82.3 | 25.6 |
| Anhui jasmine 101S/ Yang No.6 rice | 132.3 | 12.7 | 238.1 | 85.1 | 25.2 |
| The new 101S/ peaces in Anhui are No. 2 fragrant | 126.8 | 11.0 | 213.4 | 86.7 | 27.6 |
| The new 101S/ peaces perfume 15 in Anhui | 126.1 | 12.4 | 245.2 | 88.4 | 26.8 |
| The new 101S/R998 in Anhui | 127.5 | 10.3 | 239.1 | 83.2 | 26.6 |
| The new 101S/R727 in Anhui | 126.2 | 10.0 | 217.3 | 84.1 | 27.6 |
| Xian WH26 in the new 101S/ in Anhui | 119.7 | 9.2 | 226.1 | 80.9 | 27.9 |
| The new 101S/ China in Anhui accounts for | 128.2 | 9.1 | 257.1 | 85.6 | 28.5 |
| The new 101S/ Yang No.6 rice in Anhui | 128.6 | 10.5 | 233.8 | 81.5 | 27.8 |
| Purple 101S/ peaces in Anhui are No. 2 fragrant | 118.3 | 9.8 | 250.2 | 87.3 | 28.3 |
| Anhui purple 101S/ peaces perfume 15 | 119.2 | 8.7 | 247.6 | 82.8 | 28.7 |
| Anhui purple 101S/R998 | 118.5 | 10.3 | 236.4 | 84.1 | 27.9 |
| Anhui purple 101S/R727 | 120.1 | 11.7 | 216.4 | 82.9 | 28.1 |
| Xian WH26 in the purple 101S/ of Anhui | 121.5 | 12.1 | 213.8 | 84.6 | 27.8 |
| Anhui purple 101S/ China accounts for | 115.7 | 11.5 | 237.7 | 85.2 | 28.2 |
| Anhui purple 101S/ Yang No.6 rice | 116.5 | 11.0 | 226.2 | 86.7 | 27.6 |
| 913S/ peaces in Anhui are No. 2 fragrant | 122.6 | 13.2 | 232.0 | 87.2 | 28.1 |
| Anhui 913S/ peaces perfume 15 | 128.3 | 10.5 | 208.7 | 85.4 | 28.3 |
| Anhui 913S/R998 | 124.3 | 11.9 | 222.5 | 83.2 | 28.3 |
| Anhui 913S/R727 | 129.7 | 10.5 | 243.2 | 85.6 | 28.1 |
| Xian WH26 in the 913S/ of Anhui | 131.4 | 9.9 | 211.6 | 81.8 | 28.5 |
| Anhui 913S/ China accounts for | 128.4 | 9.3 | 220.9 | 86.1 | 28.4 |
| Anhui 913S/ Yang No.6 rice | 123.6 | 12.1 | 206.3 | 84.3 | 28.9 |
| It is average | 125.2 | 10.9 | 227.6 | 84.5 | 27.6 |
| Raise two excellent No. 6（CK1） | 127.1 | 10.5 | 227.8 | 86.1 | 27.4 |
| II excellent 838（CK 2） | 125.2 | 11.1 | 215.2 | 87.3 | 28.1 |
（4）Known by table 3, the effective fringe of 28 combination average single plants 10.9, between 8.7-13.2, between 2 controls；
It is average per total grain panicle number 227.6, with raise two excellent No. 6 it is basically identical, excellent higher than II 838；Average setting percentage 84.5%, it is more right than two
According to slightly lower；Average mass of 1000 kernel 27.6g, between 2 controls.It can be seen that sword-like leave stretch obliquely downward temperature-sensitive sterile line assemble it is miscellaneous
Rice varieties are handed over, yield traits are acted normally.
Claims (4)
- A kind of 1. method of portable temperature-sensitive sterile line selection and breeding, which is characterized in that this method includes the following steps：（1）It is stretched obliquely downward using the sword-like leave screened in Anhui's local kind（Openness angle of flag leaf 150o）Indica Rice Germplasm Anhui is extensive 101 with deriving from peace agriculture S（Such as wide jasmine S, Guangzhan 63S）And 1892S（Short purple S, Z913S etc.）The temperature sensitive core of rice indica hybrid not It is hybridization, selfing to educate, and obtains each combination F2For seed；（2）Each combination F2Group plants 3000 plants or so respectively, therefrom screens sword-like leave and stretches obliquely downward（Openness angle of flag leaf is more than 120o）Temperature Quick Genetic Sterility strain；（3）Screening garden is established, in low temperature（23.0-23.5℃）Under environmental pressure, by pollen microscopic examination and Fertility observation, screening is not It educates the sword-like leave that origin temp is low, plant height is less than 85cm and stretches single plant obliquely downward（Openness angle of flag leaf is more than 120o）, it is selfed up to character stabilization, The sterile gene of the low origin temp of control is made to polymerize with big openness angle of flag leaf genetic recombination；（4）By Fertility identification, it is bred as sword-like leave and stretches obliquely downward（Openness angle of flag leaf is more than 120o）Gene polymerize with temperature sensitive genic male sterile gene Big openness angle of flag leaf Indica TGMS line in same strain, the sterile line are portable temperature-sensitive sterile line.
- 2. the breeding method of the portable temperature-sensitive sterile line is required according to right 1, which is characterized in that the portable The openness angle of flag leaf of temperature-sensitive sterile line is more than 120o and plant height is less than 85cm.
- 3. it stretches the new sword-like leave of Indica TGMS line transformation obliquely downward using the sword-like leave of 1 step selection and breeding as required to stretch obliquely downward（Sword Leaf angle is more than 120o）Indica TGMS line.
- 4. application of the selection as described in claim 1 in Rice Production, which is characterized in that utilize the selection and breeding of this method institute Sword-like leave stretch Indica TGMS line obliquely downward and carry out that the artificial highly intensive labour cut needed for leaf can be saved during breeding of hybrid rice, Injury when reducing breeding cost, while avoiding cutting leaf to the tender spike of rice of children realizes that rice gently simplifies the production of hybrid seeds.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114645097A (en) * | 2022-04-27 | 2022-06-21 | 安徽省农业科学院水稻研究所 | Rice floral pillar length gene qSYL3, linked marker thereof and application thereof in breeding of rice genic male sterile line with high outcrossing maturing rate |
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| CN114645097B (en) * | 2022-04-27 | 2024-02-02 | 安徽省农业科学院水稻研究所 | Rice anther length gene qSYL3, linkage marker thereof and application thereof in breeding of rice genic male sterile line with high outcrossing seed setting rate |
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