CN104542026A - Method for screening or identifying herbicide-resistant rice via agarose matrix - Google Patents
Method for screening or identifying herbicide-resistant rice via agarose matrix Download PDFInfo
- Publication number
- CN104542026A CN104542026A CN201510013483.3A CN201510013483A CN104542026A CN 104542026 A CN104542026 A CN 104542026A CN 201510013483 A CN201510013483 A CN 201510013483A CN 104542026 A CN104542026 A CN 104542026A
- Authority
- CN
- China
- Prior art keywords
- seed
- herbicide
- rice
- imazethapyr
- sowing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Soil Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for screening or identifying herbicide-resistant rice via agarose matrix. The method for screening or identifying herbicide-resistant rice via agarose matrix comprises the following steps: soaking the seed and sprouting, preparing the culture medium, sowing, observing and counting. The method is as follows: pouring 2L of the water into the electromagnetism pot, adding 10g of agarose, stirring evenly, boiling until that the agar dissolves totally, adding the herbicide imazethapyr mother liquor, the final concentration is 0.1ml/L, shaking evenly and pouring the mixture into the flat culture dish, using after that the culture medium solidifies; marking the name of variety on the culture dish, selecting the seed with stared area for 50-55 pieces, evenly sowing the seeds on the culture medium, sowing the resistant and anti-resistant variety for two pieces for having positive and negative contrast, scribing, separating and marking; observing and counting after 3-4 days. The method can screen or identify the herbicide-resistant imazethapyr rice quickly, effectively and exactly. Very good technical means is offered for the herbicide-resistant imazethapyr rice breeding and purity identification.
Description
Technical field
The invention belongs to breed of crop field, relate to a kind of method adopting agarose matrix screening or qualification Herbicide Resistant Rice.
Background technology
Paddy rice is one of the staple food crop in China and even the world, and every Annual planting area is about 3,000 ten thousand hectares, and output reaches 200,000,000 tons.In the production process of paddy rice, weeds and paddy rice fight for resource, not only reduce rice yield, also affect the quality of rice.For controlling weed growth, a large amount of manpower and materials need be dropped into every year.Along with the quickening of urbanization process, a large amount of transfers of rural laborer, traditional production operation mode will fade away, and Rice Production will face the problem of labor shortage.Therefore, develop efficient novel cultivation mode, as rice direct-seeding, throw the developing direction that cultivation is future.But because live, throwing cultivation make Weeds distribution difficulty strengthen, and the mode of traditional-handwork weeding no longer adapts to new cropping pattern.Along with the development of science and technology, controlling weeds by chemical method has become an indispensable part in modern agriculture, chemical herbicide have save labor, efficient feature.In developed countries such as America and Europes, weed control mainly relies on chemical herbicide.Therefore by cultivating the desirable route that Herbicide Resistant Rice new varieties are solution Rice Production problems.On the other hand, utilize herbicide resistance gene also effectively can solve purity problem in conventional Rice and the double-linear hybrid rice production of hybrid seeds, and hybrid seeding program can be simplified, realize the mechanization production of hybrid seeds, reduce crossbreed cost.
But the resistance screening of current rice herbicide or qualification mainly rely on field trial to carry out, and namely sow paddy rice in field, carry out spraying of weed killer herbicide after the regular period.The method wastes time and energy, and the concentration sprayed and the uniformity wayward, thus have impact on the accuracy of result.And adopt the method cost of molecular labeling higher, and sport technique segment is many, also has certain requirement to equipment; Effective to known, and just cannot apply the screening of unknown gene and qualification.Therefore a kind of quickly, efficiently and accurately antiweed screening or authentication method just seem particularly important.
Summary of the invention
The invention discloses a kind of method adopting agarose matrix screening or qualification Herbicide Resistant Rice, it is undertaken by following step:
(1) presoaking and germinating:
Be contained in paper bag by 100-105 grain rice paddy seed, carry out mark, be immersed in the warm water of 32-34 DEG C, be placed in illumination box 32 DEG C insulation together with container, morning, noon and afternoon every day change water three times, can sow after 3-4 days show money or valuables one carries unintentionally; Described seed refers to: the rice varieties of imazethapyr resistance and the non-resistance rice varieties of imazethapyr are hybridized, and results obtain F1 generation seed; Sowing F1 generation seed, nursery, transplants, the mixed F2 seed received on F1 generation plant; Single-strain planting F2 generation and by strain results F3 for seed, acquire described rice paddy seed; Or the rice varieties seed of imazethapyr sensitivity is carried out mutagenic treatment, obtain M1 for seed; M1 is for seed in sowing, and nursery routinely, transplanting and plantation, cultivate into M1 plant, and after ripe, results obtain M2 for seed, are described rice paddy seed;
(2) medium prepares:
1) prepare herbicide mother liquor: weed killer herbicide Pu Shite is diluted ten times, namely the Pu Shite of 2ml adds water 18ml;
2) agarose plate of 0.4-0.6% (w/w) is prepared: pour in electromagnetic cooker by 2L water, add 10g agarose, stir, boil until agar dissolves completely, be dispensed into after temperature is reduced to 60-70 DEG C in triangular flask, every bottle of 500ml, add the herbicide imazethapyr mother liquor that 500ul dilutes 10 times, namely final concentration is 0.1ml/L, is down flat ware after shaking up, and can use after culture medium solidifying;
(3) sow:
Culture dish marks family name of broadcasting, choose the seed 50-55 grain showed money or valuables one carries unintentionally, uniform sowing is on medium, the kind that sowing is anti-and anti-simultaneously each two as positive and negative contrast, and mark is carried out in separation of ruling, for preventing moisture loss, by stacked together for the culture dish of 10-12 and with preservative film parcel, be finally put in the incubator of 32 DEG C of continuous illuminations;
(4) statistics is observed
After 3-4 days, carry out observation statistics, compare with anti-and anti-positive and negative check variety, the seed number of the seed number counting antiweed and not antiweed.
The resistant control kind that the present invention selects refers to: have resistant gene can not be killed (insensitive) kind by weed killer herbicide.This experiment is used: destroy 1.
The not anti-check variety that the present invention selects refers to: do not have resistant gene can be killed the kind of (sensitivity) by weed killer herbicide.This experiment is used: Japan is fine.
Seed of the present invention, typical rice paddy seed to be measured can be obtained by mutation breeding or crossbreeding two kinds of methods:
When adopting mutation breeding, described rice paddy seed can be cultivated by the following method: the rice varieties seed of imazethapyr sensitivity is carried out mutagenic treatment, obtains M1 for seed; M1 is for seed in sowing, and nursery routinely, transplanting and plantation, cultivate into M1 for plant, and after ripe, results obtain M2 for seed, are described rice paddy seed.
Wherein, described mutagenic treatment can adopt physical mutagen or chemical mutagen one wherein or combination to carry out.Described physical mutagen is gamma-rays, energetic ion etc.; Gamma-rays processing method: utilize in professional irradiation unit
60co gamma-rays is mutation source, with 300 Gy treatment dosage process dry seedses.Described chemical mutagen is sodium azide (NaN
3), methyl methylsulfonate (MMS), ethylmethane sulfonate (EMS) etc.; EMS processing method: with distilled water immersion seed 5 h, change 1% EMS solution soaked overnight (about 12 h), and clear water rinses 4 h.
When adopting crossbreeding, described rice paddy seed is cultivated by the following method: the paddy rice cross breeding rice varieties of imazethapyr resistance and imazethapyr not resisted, and results obtain F1 generation seed; F1 is for seed in sowing, and nursery according to a conventional method, transplanting and plantation, cultivate into F1 plant, and after ripe, results obtain F2 for seed, single-strain planting F2 generation and by strain results F3 for seed, acquire described rice paddy seed.
Paper bag of the present invention refers to sulfuric acid paper bag (having another name called kraft paper bag), and described plate diameter is 9cm, 30ml/ ware.
The concentration of preferred preparation agarose is 0.5% (w/w).
The present invention can quickly, efficiently and accurately screening or identify the paddy rice of antiweed imazethapyr.For antiweed imazethapyr rice breeding and Purity provide good technological means.
The present invention utilizes and carries out screening or identifying containing certain density imazethapyr agarose media in the culture dish in laboratory, and rice growing is carried out the process of spray medicine and screens or identify by prior art to field.
The present invention typically discloses herbicide imazethapyr, and other the weed killer herbicide with same nature (inner sucting conduction type) can be undertaken by the method.As glyphosate, prometryn, cover grass energy with surely kill ether etc.
Invention key point of the present invention is: added by weed killer herbicide in agarose and carry out paddy rice cultivation in culture dish, the result display of cultivation:
(1) this method well can differentiate anti-and not anti-rice varieties and strain, or resistance individual plant.
(2) this method technical system is perfect, and after especially adding positive and negative contrast, result is very accurate.
(3) this method is time saving and energy saving, and requirement for experiment condition is low, is suitable for Large-scale Screening or qualification.
Accompanying drawing illustrates:
Fig. 1 is rice paddy seed Resistance Identification result; Wherein Figure 1A-C is individual plant results seed resistance qualification result; Illustrate that the method can accurately identify the Herbicid resistant situation of previous generation individual plant; Fig. 1 D is the selection result of resistant strain after mutagenesis; Illustrate that the method can effectively filter out resistance individual plant;
Fig. 2 is the cultivation situation map in different culture environment; Wherein Fig. 2 A is that illumination box cultivates situation, and Fig. 2 B is laboratory cultures situation; Illustrate that the method can be cultivated in the laboratory with certain constant temperature again at illumination box, and requisite space is little, illumination box can primary screening more than 1,000 parts materials;
Fig. 3 is that herbicide imazethapyr concentration gradient carries out Screening and Identification lab diagram across 0 – 4 ml/L; Fig. 3 illustrates that herbicide concentration all can carry out screening or identifying in 0.01-0.5 ml/L scope.Less demanding to herbicide concentration of the method is described, and the preferred concentration of 0.1ml/L can ensure to test accurately carrying out.
Embodiment
In order to explain enforcement of the present invention more fully, provide following method embodiment.These embodiments are only explain instead of limit the scope of the invention.Wherein test the seed used to destroy 1 and all have commercially available with Tianjin rice 263, golden round-grained rice 28 and weed killer herbicide Pu Shite.
Embodiment 1
Agarose matrix qualification is adopted to cultivate the method for Herbicide Resistant Rice:
(1) rice paddy seed to be measured obtains
Imazethapyr Sensitive Rice is destroyed 1 and imazethapyr insensitive paddy rice Tianjin rice 263 be sowed at field, to Tianjin rice 263 emasculation during full-bloom stage, and hybridize with the pollen of destroying 1, results F1 generation seed.Plant F1 generation seed, the mixed F2 that receives is for seed; Single-strain planting F2 is for plant, and F3 is for seed for individual plant results.
(2) presoaking and germinating
Above-mentioned 100 F3 are withheld the seed obtained to be contained in sulfuric acid paper bag, carries out mark to different individual plants, is immersed in the warm water of 32 DEG C, and be placed in illumination box 32 DEG C insulation together with container, morning, noon and afternoon every day change water three times, sows after 3 days show money or valuables one carries unintentionally.
(3) medium prepares
1) prepare herbicide mother liquor: weed killer herbicide Pu Shite is diluted ten times, namely the Pu Shite of 2ml adds water 18ml.
2) agarose plate of 0.5% (w/w) is prepared: pour in electromagnetic cooker by 2L water, add 10g agarose, stir, boil until agar dissolves completely, be dispensed into after temperature is reduced to 70 DEG C in triangular flask, every bottle of 500ml, add the herbicide imazethapyr mother liquor that 500ul dilutes 10 times, ultimate density is 0.1ml/L, ware is down flat after shaking up, diameter is 9cm, 30ml/ ware, can use after culture medium solidifying.
(4) sow
Mark individual plant of broadcasting numbering on culture dish, choose 55, the seed showed money or valuables one carries unintentionally, uniform sowing is on medium, the kind that sowing is anti-and anti-simultaneously each two as positive and negative contrast, and mark is carried out in separation of ruling, for preventing moisture loss, by stacked together for the culture dish of 10-12 and with preservative film parcel, be finally put in the incubator of 32 DEG C of continuous illuminations.
(5) statistics is observed
After 3-4 days, carry out observation statistics, compare with anti-and anti-positive and negative check variety, the seed number of the seed number counting antiweed and not antiweed.
Result:
This example will isozygoty to individual plant and the qualification of heterozygosis, meets mendel's law, and namely the seed of homozygous strain is all resistance or anti-seed (Figure 1A and 1B), and the seed of heterozygosis strain meet anti-ly be about 3:1(Fig. 1 C with not anti-seed number ratio).
Embodiment 2
Agarose matrix screening is adopted to cultivate the method for Herbicide Resistant Rice:
(1) rice paddy seed to be measured obtains
By 1kg gold round-grained rice 28 seed distilled water immersion 5 h, change 1% EMS solution soaked overnight (about 12 h), and clear water rinses 4 h, then overnight incubation in the water of 32 DEG C is immersed in, within 2nd day, sowing is in rice seedling bed, transplants to land for growing field crops, obtain M1 for seed after maturation after growing 4-5 blade.Carried out sowing for seed by all M1, individual plant transplants, the M2 on results individual plant (can many strains are mixed receives for reducing workload) is for seed.
(2) presoaking and germinating
Be contained in sulfuric acid paper bag for seed, carry out mark, be immersed in the warm water of about 32 DEG C by above-mentioned 100 individual plants (or the mixed receipts of many strains) M2, be placed in illumination box 32 DEG C insulation together with container, morning, noon and afternoon every day change water three times.Can sow after 4 days show money or valuables one carries unintentionally.
(3) medium prepares
1) prepare herbicide mother liquor: weed killer herbicide Pu Shite is diluted ten times, namely the Pu Shite of 2ml adds water 18ml;
2) agarose plate of 0.5% (w/w) is prepared: pour in electromagnetic cooker by 2L water, add 10g agarose, stir, boil until agar dissolves completely, be dispensed into after temperature is reduced to 70 DEG C in triangular flask, every bottle of 500ml, add the herbicide imazethapyr mother liquor that 500ul dilutes 10 times, ultimate density is 0.1ml/L, ware is down flat after shaking up, diameter is 9cm, 30ml/ ware, can use after culture medium solidifying.
(4) sow
Mark individual plant of broadcasting numbering on culture dish, choose 55, the seed showed money or valuables one carries unintentionally, uniform sowing is on medium, the kind that sowing is anti-and anti-simultaneously each two as positive and negative contrast, and mark is carried out in separation of ruling, for preventing moisture loss, by stacked together for the culture dish of 10-12 and with preservative film parcel, be finally put in the incubator of 32 DEG C of continuous illuminations.
(5) result observes statistics
After 3-4 days, carry out observation statistics, compare with anti-and not anti-positive and negative check variety, filter out resistance individual plant.
Points for attention:
(1) the positive and negative check variety soaking q.s is noted during seed soaking.
(2) batch different hardness of agarose is also different, notes adjustment, generally adjusts between 0.4%-0.6%.
Easily fade in Marker making glass vessel incubation, therefore the most handy plastic ware; Person's handwriting on paper bag be also easily immersed in water fuzzy fall.
5, result
As Fig. 1 D, the method can effectively filter out resistance individual plant.
The method compares with conventional method:
Claims (4)
1. adopt agarose matrix to screen or identify a method for Herbicide Resistant Rice, it is characterized in that being undertaken by following step:
(1) presoaking and germinating:
Be contained in paper bag by 100-105 grain rice paddy seed, carry out mark, be immersed in the warm water of 32-34 DEG C, be placed in illumination box 32 DEG C insulation together with container, morning, noon and afternoon every day change water three times, can sow after 3-4 days show money or valuables one carries unintentionally; Described rice paddy seed refers to: the rice varieties of imazethapyr resistance and the non-resistance rice varieties of imazethapyr are hybridized, and results obtain F1 generation seed; Sowing F1 generation seed, nursery, transplants, the mixed F2 seed received on F1 generation plant; Single-strain planting F2 generation and by strain results F3 for seed, acquire described rice paddy seed; Or the rice varieties seed of imazethapyr sensitivity is carried out mutagenic treatment, obtain M1 for seed; M1 is for seed in sowing, and nursery routinely, transplanting and plantation, cultivate into M1 plant, and after ripe, results obtain M2 for seed, are described rice paddy seed;
(2) medium prepares:
1) prepare herbicide mother liquor: herbicide imazethapyr is diluted ten times, namely the Pu Shite of 2ml adds water 18ml;
2) agarose plate of 0.4-0.6% (w/w) is prepared: pour in electromagnetic cooker by 2L water, add 10g agarose, stir, boil until agar dissolves completely, be dispensed into after temperature is reduced to 60-70 DEG C in triangular flask, every bottle of 500ml, add the herbicide imazethapyr mother liquor that 500ul dilutes 10 times, namely final concentration is 0.1ml/L, is down flat ware after shaking up, and can use after culture medium solidifying;
(3) sow:
Culture dish marks family name of broadcasting, choose the seed 50-55 grain showed money or valuables one carries unintentionally, uniform sowing is on medium, the kind that sowing is anti-and anti-simultaneously each two as positive and negative contrast, and mark is carried out in separation of ruling, for preventing moisture loss, by stacked together for the culture dish of 10-12 and with preservative film parcel, be finally put in the incubator of 32 DEG C of continuous illuminations and cultivate;
(4) statistics is observed:
After 3-4 days, carry out observation statistics, compare with anti-and anti-positive and negative check variety, the seed number of the seed number counting antiweed and not antiweed.
2. method according to claim 1, paper bag wherein refers to sulfuric acid paper bag.
3. method according to claim 1, plate diameter is wherein 9cm, 30ml/ ware.
4. method according to claim 1, the concentration wherein preparing agarose is 0.5% (w/w).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510013483.3A CN104542026A (en) | 2015-01-12 | 2015-01-12 | Method for screening or identifying herbicide-resistant rice via agarose matrix |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510013483.3A CN104542026A (en) | 2015-01-12 | 2015-01-12 | Method for screening or identifying herbicide-resistant rice via agarose matrix |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104542026A true CN104542026A (en) | 2015-04-29 |
Family
ID=53059964
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510013483.3A Pending CN104542026A (en) | 2015-01-12 | 2015-01-12 | Method for screening or identifying herbicide-resistant rice via agarose matrix |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104542026A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522077A (en) * | 2018-05-31 | 2018-09-14 | 福建省农业科学院植物保护研究所 | Sowing for the identification of rice varieties disease resistance dispenses component |
| CN109673508A (en) * | 2019-02-20 | 2019-04-26 | 安徽绿亿种业有限公司 | Make method of the rice with antiweed function by mutation breeding |
| CN115428727A (en) * | 2022-09-29 | 2022-12-06 | 江苏省农业科学院宿迁农科所 | Method for breeding herbicide-resistant rice by utilizing culture dish for seedling raising precision identification |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6485024A (en) * | 1987-06-01 | 1989-03-30 | Hokko Chem Ind Co | Production of rice variety resistant to herbicide, said rice plant and its seed |
| CN104255403A (en) * | 2014-08-28 | 2015-01-07 | 兰州大学 | Screening method for glyphosate-resisting medicago sativa plants |
-
2015
- 2015-01-12 CN CN201510013483.3A patent/CN104542026A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6485024A (en) * | 1987-06-01 | 1989-03-30 | Hokko Chem Ind Co | Production of rice variety resistant to herbicide, said rice plant and its seed |
| CN104255403A (en) * | 2014-08-28 | 2015-01-07 | 兰州大学 | Screening method for glyphosate-resisting medicago sativa plants |
Non-Patent Citations (4)
| Title |
|---|
| 刘湘林 等: "不同品种水稻对乙草胺的敏感性研究", 《中国农学通报》 * |
| 朱金文 等: "五氟磺草胺对水稻种子萌发及幼苗生长的影响", 《农药学学报》 * |
| 王炽: "杂交水稻种子浸种催芽实用技术", 《现代农业科技》 * |
| 马林 等: "抗除草剂转基因水稻和大豆快速准确检测技术研究", 《浙江大学学报（农业与生命科学版）》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108522077A (en) * | 2018-05-31 | 2018-09-14 | 福建省农业科学院植物保护研究所 | Sowing for the identification of rice varieties disease resistance dispenses component |
| CN109673508A (en) * | 2019-02-20 | 2019-04-26 | 安徽绿亿种业有限公司 | Make method of the rice with antiweed function by mutation breeding |
| CN115428727A (en) * | 2022-09-29 | 2022-12-06 | 江苏省农业科学院宿迁农科所 | Method for breeding herbicide-resistant rice by utilizing culture dish for seedling raising precision identification |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ashraf et al. | Wheat seed germination under low temperature and moisture stress 1 | |
| Sabar et al. | Identification and mapping of QTLs associated with drought tolerance traits in rice by a cross between Super Basmati and IR55419-04 | |
| Yan et al. | Yield formation and tillering dynamics of direct-seeded rice in flooded and nonflooded soils in the Huai River Basin of China | |
| Wu et al. | Root growth in response to nitrogen supply in Chinese maize hybrids released between 1973 and 2009 | |
| CN102835297A (en) | Identification method of salt tolerance of barley | |
| CN109819714A (en) | A kind of method that can largely screen rice seedling Salinity tolerance germplasm | |
| CN108849528A (en) | A method of obtaining eremochloa ophiuroides mutant | |
| McGrail et al. | Two centuries of breeding has altered root system architecture of winter wheat | |
| CN104542026A (en) | Method for screening or identifying herbicide-resistant rice via agarose matrix | |
| Wakeley | Artificial reforestation in the southern pine region | |
| Aufhammer et al. | Germination of grain amaranth (Amaranthus hypochondriacus× A. hybridus): effects of seed quality, temperature, light, and pesticides | |
| CN112375840B (en) | Major QTL for regulating and controlling resistance of rice sogatella furcifera, molecular marker and application | |
| Rudell et al. | Integrated weed management strategies in a long-term crop rotation system | |
| CN104303989B (en) | A kind of hybrid rice seed breeding process | |
| CN107593430A (en) | A kind of hybrid rice variety seed selection of suitable miscegenation production of hybrid seeds and seed production methods | |
| Sharma | Lac insect-host plant interaction: Implications on quantity and quality of lac | |
| CN116622898A (en) | Method for rapidly screening low-cadmium mutant in rice mutant library by using OsNramp5 gene | |
| CN106472141A (en) | A kind of method of Artificial disease nursery Rapid identification tobacco black shank resistance | |
| Madhav et al. | Phenotyping rice for molecular plant breeding | |
| Jiang et al. | Preliminary studies on the tissue culture of Cannabis sativa L.(Industrial hemp). | |
| CN103651078A (en) | Method for screening transgenic plant seeds | |
| CN101427651A (en) | Cultivation method for dual-purpose hybrid rice for flood and drought | |
| CN106718828A (en) | A kind of breeding method of drought-enduring type rice varieties | |
| CN101810138A (en) | Breeding method of novel variety of dwarf bermudagrass | |
| CN108739196A (en) | A kind of soybean salt-tolerance composite sifting and identification method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150429 |