CN104488700A - Breeding method of CMS lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice - Google Patents
Breeding method of CMS lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice Download PDFInfo
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- CN104488700A CN104488700A CN201510020053.4A CN201510020053A CN104488700A CN 104488700 A CN104488700 A CN 104488700A CN 201510020053 A CN201510020053 A CN 201510020053A CN 104488700 A CN104488700 A CN 104488700A
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Abstract
The invention discloses a breeding method of CMS lines of purple-leaf rice, erect-ear rice, uppermost internode rice and glutinous rice and belongs to the technical field of special rice breeding. The breeding method is characterized in that purple-leaf rice maintainer line Xianhong B, erect-ear rice variety 259, uppermost internode rice three-line maintainer line IR58025eB (1) or IR68902eB and glutinous rice three-line maintainer line Jianong wxB1 are adopted as parents and are converged into three-line maintainer lines through hybridization, multiple cross, convergent backcross and other methods, and after hybridization and backcross with the corresponding sterile lines, four CMS lines which have excellent agronomic traits and yield traits as well as special beneficial traits of the four rice varieties can be bred. The glutinous rice CMS lines have the advantages of being high in purity and seed production yield and needing little gibberellin.
Description
Technical field
The present invention is specifically related to the selection of the glutinous CMS line of a kind of purple leaf upright spike length fringe neck, belongs to Special rice breeding technical field.
Background technology
Paddy rice, as the first cereal crops of China, makes a great contribution to solution Food Security in China and raising people ' s health level.Glutinous rice is one of traditional grain crop of China, and be also the important source material that non-staple food is produced, the seed selection of glutinous rice has broad application prospects.At present, the glutinous rice variety in production is still based on conventional glutinous rice, and waxy hybrid rice combination is less, and the triple crossing glutinous rice combination identity of seed selection to assembly high yield and high quality of glutinous rice CMS line is great.CMS line also exists the defect of inheritance Bao Sui, seed produces must use gibberellin, not only increase the cost of seed produces, also create environmental pollution, seed selection long fringe neck CMS line can reach heredity to be removed or alleviates Bao Sui, use no or little gibberellin, thus the seed produces cost of hybrid rice is declined, seed quality improves and reduces environmental pollution.The rice panicle type group structure of erect head type is reasonable, and the ecological conditions such as canopy intensity of illumination, temperature, humidity are superior, is conducive to improving fertilizer resistance anti-lodging, also helps pollen transmission, improve outcrossing seed-setting rate.Two-line sterile line of rice occurs replying because fertility is subject to the impact of anomalous weather, causes hybrid F
1purity decline in various degree, production of hybrid seeds production risk coefficient is higher.Using purple leaf gene by hybridization method transformation to CMS line as mark property, mix plant to remove, can greatly improve hybrid F
1purity.Purple leaf, erect head, long fringe neck and these 4 kinds of proterties glutinous are aggregated in CMS line simultaneously, the glutinous rice CMS line possessing multiple advantage can be bred as.
Summary of the invention
The object of the present invention is to provide the selection of the glutinous CMS line of a kind of purple leaf upright spike length fringe neck, thus improve seed production purity, the hybrid seed yield of glutinous two-line sterile line, reduce breeding cost.This method is with purple leaf paddy rice maintainer first red B, erect head rice varieties 259(opens book label etc. the cultivation of long-grained nonglutinous rice erect head mutant, the genetic analysis of qualification and mutant character thereof. nuclear agricultural science report, 2007, 21 (3): 209-211), long fringe neck three is maintainer IR58025eB (1) or IR68902eB (1), glutinous three is that to praise agriculture wxB1 be parent to maintainer, by hybridization, reestablish diplomatic relations and backcross polymerization by purple leaf, erect head, it is in maintainer that long fringe neck and these 4 kinds of proterties glutinous are aggregated to three, hybridize through itself and corresponding male sterile line again and backcross, be bred as economical character and yield traits excellence, there is purple leaf, erect head, the CMS line of long fringe neck and waxy 4 kinds of special beneficial characteristics.
For reaching above object, the present invention by the following technical solutions:
1) select purple leaf paddy rice maintainer first red B(A) (Deng Guofu etc. the first seed selection of red A of rice purple red autumnal leaves marking sterile series and application. " Chinese rice " 05 phase: 29-31 in 2008), erect head rice varieties 259(B), long fringe neck three be maintainer IR58025eB (1) or IR68902eB (1) (C), glutinous three is that maintainer praises agriculture wxB1(D), make hybridization A/B and C/D respectively, obtain first familiar generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1200-1400 strain, carries out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plantation 40-50 strain, plant glutinous three is maintainer D simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, the individual plant 5-10 of economical character excellence and glutinous three is therefrom selected to be that maintainer D(makes female parent) hybridize, must F be hybridized
1in generation, with BF
1-ABCDrepresent;
6) 5 are planted) gained BF
1-ABCDseed, selfing obtains BF
2-ABCD, strain plantation BF
2-ABCD, colony, in 600-800 strain, carries out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, maturing stage is individual plant sowing respectively, obtains BF
3-ABCDseed;
7) 6 are planted) gained BF
3-ABCDseed, selects the BF of economical character excellence flowering stage
3-ABCDindividual plant 5 and glutinous three is that maintainer D(makes female parent) backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony is in 500-600 strain, carry out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have simultaneously long fringe neck and erect head proterties, economical character excellent, be the most similar individual plant 10 strain listing mark of maintainer D to glutinous three;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, yield traits, pollen fertility, select economical character and glutinous three be maintainer D similar, there is purple leaf, long fringe neck and erect head proterties, can be fixed to purple leaf upright spike length fringe neck glutinous three be maintainer " ZZewxB " for individual plant 5 strain that pollen loose powder is good;
9) " ZZewxB " and glutinous three is that male sterile line that maintainer praises agriculture wxB1 is praised agriculture wxA1 and hybridized and the 2-3 generation that backcrosses, and can be bred as the purple leaf upright spike length glutinous CMS line of fringe neck " ZZewxA ".
The selection of the glutinous CMS line of purple leaf upright spike length fringe neck, its glutinous CMS line of purple leaf upright spike length fringe neck be bred as has the characteristic of purple leaf proterties, erect head proterties, long fringe neck proterties and waxy proterties simultaneously.
beneficial effect of the present invention:
Effect of the present invention is respectively the gene controlling purple leaf, erect head, long fringe neck and these 4 kinds of proterties glutinous to be backcrossed and triage techniques through many generation hybridization, be aggregated in same Three-line rice male sterile line, be bred as the CMS line that agronomy and yield traits are excellent, have purple leaf, erect head, long fringe neck and waxy 4 kinds of special beneficial characteristics, this glutinous rice CMS line has that male sterile line purity is high, hybrid seed yield is high, hybrid F
1purity is high, the advantage such as gibberellin, decreasing pollution is beaten in the production of hybrid seeds less, and every mu of seed farm can reduce costs 70-90 unit, increase yield 5-8%.
Embodiment
Embodiment 1:
1) select purple leaf paddy rice maintainer first red B(A), erect head rice varieties 259(B), long fringe neck three be maintainer IR58025eB (1) (C), glutinous three is that maintainer praises agriculture wxB1(D), make hybridization A/B and C/D respectively, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1200 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plants 40 strains, plant glutinous three is maintainer D simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, the individual plant 5 and glutinous three of economical character excellence is therefrom selected to be that maintainer D(makes female parent) hybridize, must F be hybridized
1in generation, with BF
1-ABCDrepresent;
6) 5 are planted) gained BF
1-ABCDseed, selfing obtains BF
2-ABCD, strain plantation BF
2-ABCD, colony, in 600 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains BF
3-ABCDseed;
7) 6 are planted) gained BF
3-ABCDseed, selects the BF of economical character excellence flowering stage
3-ABCDindividual plant 5 and glutinous three is that maintainer D(makes female parent) backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony, in 500 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have simultaneously long fringe neck and erect head proterties, economical character excellent, be the most similar individual plant 10 strain listing mark of maintainer D to glutinous three;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, yield traits, pollen fertility, select economical character and glutinous three be maintainer D similar, there is purple leaf, long fringe neck and erect head proterties, can be fixed to purple leaf upright spike length fringe neck glutinous three be maintainer " ZZewxB-1 " for individual plant 5 strain that pollen loose powder is good;
9) " ZZewxB-1 " and glutinous three is that male sterile line that maintainer praises agriculture wxB1 is praised agriculture wxA1 and hybridized and 3 generations that backcrossed, and can be bred as the purple leaf upright spike length glutinous CMS line of fringe neck " ZZewxA-1 ".
The purple leaf upright spike length glutinous CMS line of fringe neck " ZZewxA-1 " has that male sterile line purity is high, hybrid seed yield is high, hybrid F
1purity is high, the advantage such as gibberellin, decreasing pollution is beaten in the production of hybrid seeds less, and every mu of seed farm can reduce costs 70 yuan, increase yield 5%.
Embodiment 2:
1) select purple leaf paddy rice maintainer first red B(A), erect head rice varieties 259(B), long fringe neck three be maintainer IR68902eB (1) (C), glutinous three is that maintainer praises agriculture wxB1(D), make hybridization A/B and C/D respectively, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1400 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plants 50 strains, plant glutinous three is maintainer D simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, the individual plant 10 and glutinous three of economical character excellence is therefrom selected to be that maintainer D(makes female parent) hybridize, must F be hybridized
1in generation, with BF
1-ABCDrepresent;
6) 5 are planted) gained BF
1-ABCDseed, selfing obtains BF
2-ABCD, strain plantation BF
2-ABCD, colony, in 800 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carries out erect head and long fringe neck Characters Identification flowering stage, and select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, the maturing stage is individual plant sowing respectively, obtains BF
3-ABCDseed;
7) 6 are planted) gained BF
3-ABCDseed, selects the BF of economical character excellence flowering stage
3-ABCDindividual plant 5 and glutinous three is that maintainer D(makes female parent) backcross, obtain 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony, in 600 strains, carries out purple leaf Characters Identification seedling stage, removes the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have simultaneously long fringe neck and erect head proterties, economical character excellent, be the most similar individual plant 10 strain listing mark of maintainer D to glutinous three;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, yield traits, pollen fertility, select economical character and glutinous three be maintainer D similar, there is purple leaf, long fringe neck and erect head proterties, can be fixed to purple leaf upright spike length fringe neck glutinous three be maintainer " ZZewxB-2 " for individual plant 5 strain that pollen loose powder is good;
9) " ZZewxB-2 " and glutinous three is that male sterile line that maintainer praises agriculture wxB1 is praised agriculture wxA1 and hybridized and 2 generations that backcrossed, and can be bred as the purple leaf upright spike length glutinous CMS line of fringe neck " ZZewxA-2 ".
The purple leaf upright spike length glutinous CMS line of fringe neck " ZZewxA-2 " has that male sterile line purity is high, hybrid seed yield is high, hybrid F
1purity is high, the advantage such as gibberellin, decreasing pollution is beaten in the production of hybrid seeds less, and every mu of seed farm can reduce costs 90 yuan, increase yield 8%.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (2)
1. the selection of the glutinous CMS line of purple leaf upright spike length fringe neck, is characterized in that: described selection comprises the following steps:
1) select purple leaf paddy rice maintainer first red B(A), erect head rice varieties 259(B), long fringe neck three be maintainer IR58025eB (1) or IR68902eB (1) (C), glutinous three is that maintainer praises agriculture wxB1(D), make hybridization A/B and C/D respectively, must F be hybridized
1in generation, respectively with F
1-ABand F
1-CDrepresent;
2) F is hybridized in plantation respectively
1for F
1-ABand F
1-CD, make hybridization F in flowering stage
1-CD/ F
1-AB, must reestablish diplomatic relations F
1, with F
1-ABCDrepresent;
3) F is planted
1-ABCD, selfing sowing obtains F
2-ABCD, plantation F
2-ABCD, colony, in 1200-1400 strain, carries out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, maturing stage is individual plant sowing respectively, obtains F
3-ABCDseed;
4) by 3) gained F
3-ABCDseed shells out rough, selects the brown rice grain with glutinous rice proterties;
5) strain plantation 4) gained has the F of purple leaf, long fringe neck, erect head and waxy shape
3-ABCDsingle-strain seed, each strain plantation 40-50 strain, plant glutinous three is maintainer D simultaneously, flowering stage is identified each strain, select to show purple leaf, long fringe neck with erect head proterties and without the strain be separated simultaneously, therefrom select the individual plant 5-10 of economical character excellence and glutinous three to be that maintainer D is hybridized, glutinous three is that maintainer D makes female parent, must hybridize F
1in generation, with BF
1-ABCDrepresent;
6) 5 are planted) gained BF
1-ABCDseed, selfing obtains BF
2-ABCD, strain plantation BF
2-ABCD, colony, in 600-800 strain, carries out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select the rice strain listing mark simultaneously with long fringe neck and erect head proterties, maturing stage is individual plant sowing respectively, obtains BF
3-ABCDseed;
7) 6 are planted) gained BF
3-ABCDseed, selects the BF of economical character excellence flowering stage
3-ABCDindividual plant 5 and glutinous three is that maintainer D backcrosses, and glutinous three is that maintainer D makes female parent, obtains 5 BC that backcross
1f
1generation, plantation BC
1f
1for seed, selfing obtains BC
1f
2generation, plantation BC
1f
2generation, colony is in 500-600 strain, carry out purple leaf Characters Identification seedling stage, remove the rice shoot of non-purple leaf proterties, carry out erect head and long fringe neck Characters Identification flowering stage, select to have simultaneously long fringe neck and erect head proterties, economical character excellent, be the most similar individual plant 10 strain listing mark of maintainer D to glutinous three;
8) strain plantation 7) selected 10 individual plants, investigation screening is carried out to its economical character, yield traits, pollen fertility, select economical character and glutinous three be maintainer D similar, there is purple leaf, long fringe neck and erect head proterties, can be fixed to purple leaf upright spike length fringe neck glutinous three be maintainer " ZZewxB " for individual plant 5 strain that pollen loose powder is good;
9) " ZZewxB " and glutinous three is that male sterile line that maintainer praises agriculture wxB1 is praised agriculture wxA1 and hybridized and the 2-3 generation that backcrosses, and can be bred as the purple leaf upright spike length glutinous CMS line of fringe neck " ZZewxA ".
2. the selection of the glutinous CMS line of purple leaf according to claim 1 upright spike length fringe neck, it is characterized in that: the glutinous CMS line of purple leaf upright spike length fringe neck of incubation has purple leaf proterties, erect head proterties, long fringe neck proterties and waxy proterties simultaneously, and purity is high, hybrid seed yield is high, beat gibberellin advantage less.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818457A (en) * | 2017-01-22 | 2017-06-13 | 福建农林大学 | A kind of selection of loose panicle type perfume rice CMS line |
| CN107801629A (en) * | 2017-11-01 | 2018-03-16 | 安徽友鑫农业科技有限公司 | A kind of round-grained rice two is the selection of temperature and light sensitive nuclear sterility system |
| CN109964807A (en) * | 2019-03-29 | 2019-07-05 | 福建省农业科学院水稻研究所 | A kind of breeding method of the black glutinous CMS line of Erect Panicle |
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| CN103891603A (en) * | 2014-04-04 | 2014-07-02 | 福建农林大学 | Breeding method for multifunctional rice three-line sterile line |
| CN104126497A (en) * | 2014-07-14 | 2014-11-05 | 福建农林大学 | Method for breeding high-stress-resistance giant embryo rice sterile line |
| CN104137764A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of three-line genic male sterile line of high-resistance glabrous yellow-leaf giant embryo rice |
| CN104137767A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of high-stress resistance elongated upper internode three-line genic male sterile line |
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2015
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891603A (en) * | 2014-04-04 | 2014-07-02 | 福建农林大学 | Breeding method for multifunctional rice three-line sterile line |
| CN104126497A (en) * | 2014-07-14 | 2014-11-05 | 福建农林大学 | Method for breeding high-stress-resistance giant embryo rice sterile line |
| CN104137764A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of three-line genic male sterile line of high-resistance glabrous yellow-leaf giant embryo rice |
| CN104137767A (en) * | 2014-07-14 | 2014-11-12 | 福建农林大学 | Breeding method of high-stress resistance elongated upper internode three-line genic male sterile line |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106818457A (en) * | 2017-01-22 | 2017-06-13 | 福建农林大学 | A kind of selection of loose panicle type perfume rice CMS line |
| CN106818457B (en) * | 2017-01-22 | 2018-10-23 | 福建农林大学 | A kind of selection of loose panicle type perfume (or spice) rice CMS line |
| CN107801629A (en) * | 2017-11-01 | 2018-03-16 | 安徽友鑫农业科技有限公司 | A kind of round-grained rice two is the selection of temperature and light sensitive nuclear sterility system |
| CN107801629B (en) * | 2017-11-01 | 2021-01-15 | 安徽友鑫农业科技有限公司 | Breeding method of japonica two-line temperature-sensitive genic male sterile line |
| CN109964807A (en) * | 2019-03-29 | 2019-07-05 | 福建省农业科学院水稻研究所 | A kind of breeding method of the black glutinous CMS line of Erect Panicle |
| CN109964807B (en) * | 2019-03-29 | 2022-03-25 | 福建省农业科学院水稻研究所 | Method for cultivating upright ear type black glutinous rice three-line sterile line |
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| CN104488700B (en) | 2016-01-06 |
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